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Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 Fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 Produktinformation Diagnostik & molekulare Diagnostik Laborgeräte & Service Zellkultur & Verbrauchsmaterial Forschungsprodukte & Biochemikalien Weitere Information auf den folgenden Seiten! See the following pages for more information! Lieferung & Zahlungsart Lieferung: frei Haus Bestellung auf Rechnung SZABO-SCANDIC Lieferung: € 10,- HandelsgmbH & Co KG Erstbestellung Vorauskassa Quellenstraße 110, A-1100 Wien T. +43(0)1 489 3961-0 Zuschläge F. +43(0)1 489 3961-7 [email protected] • Mindermengenzuschlag www.szabo-scandic.com • Trockeneiszuschlag • Gefahrgutzuschlag linkedin.com/company/szaboscandic • Expressversand facebook.com/szaboscandic SAN TA C RUZ BI OTEC HNOL OG Y, INC . BZW1 shRNA (h) Lentiviral Particles: sc-94750-V BACKGROUND APPLICATIONS BZW1 (basic leucine zipper and W2 domains 1), also known as BZAP45, is a BZW1 shRNA (h) Lentiviral Particles is recommended for the inhibition of 419 amino acid member of the BZW family that contains one W2 domain and BZW1 expression in human cells. enhances histone H4 gene transcription. Existing as two alternatively spliced isoforms, BZW1 is expressed in day 3 embryos and is encoded by a gene that SUPPORT REAGENTS maps to human chromosome 2q33.1. Chromosome 2 consists of 237 mil lion Control shRNA Lentiviral Particles: sc-108080. Available as 200 µl frozen bases, encodes over 1,400 genes and makes up approximately 8% of the viral stock containing 1.0 x 10 6 infectious units of virus (IFU); contains an human genome. A number of genetic diseases are linked to genes on chro - shRNA construct encoding a scrambled sequence that will not lead to the mosome 2. Harlequin icthyosis, a rare and morbid skin deformity, is associat - specific degradation of any known cellular mRNA. ed with mutations in the ABCA12 gene. The lipid metabolic disorder sitos - terolemia is associated with ABCG5 and ABCG8. An extremely rare reces sive GENE EXPRESSION MONITORING genetic disorder, Alström syndrome is due to mutations in the ALMS1 gene. BZW1 (S-14): sc-102657 is recommended as a control antibody for monitor - REFERENCES ing of BZW1 gene expression knockdown by Western Blotting (starting dilu - tion 1:200, dilution range 1:100-1:1000) or immunofluorescence (starting 1. Patel, S.B., et al. 1998. Mapping a gene involved in regulating dietary cho - dilution 1:50, dilution range 1:50-1:500). lesterol absorption. The sitosterolemia locus is found at chromosome 2p21. J. Clin. Invest. 102: 1041-1044. To ensure optimal results, the following support (secondary) reagents are recommended: 1) Western Blotting: use goat anti-rabbit IgG-HRP: sc-2004 2. Zumsteg, U., et al. 2000. Alstrom syndrome: confirmation of linkage to (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible goat anti- chromosome 2p12-13 and phenotypic heterogeneity in three affected sibs. rabbit IgG-HRP: sc-2030 (dilution range: 1:2000-1:5000), Cruz Marker™ J. Med. Genet. 37: E8. Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: 3. Mitra, P., et al. 2001. Purification and functional analysis of a novel leucine- sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- zipper/nucleotide-fold protein, BZAP45, stimulating cell cycle regulated rescence: use goat anti-rabbit IgG-FITC: sc-2012 (dilution range: 1:100- histone H4 gene transcription. Biochemistry 40: 10693-10699. 1:400) or goat anti-rabbit IgG-TR: sc-2780 (dilution range: 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941. 4. Shulenin, S., et al. 2001. An ATP-binding cassette gene (ABCG5) from the ABCG (White) gene subfamily maps to human chromosome 2p21 in the RT-PCR REAGENTS region of the Sitosterolemia locus. Cytogenet. Cell Genet. 92: 204-208. Semi-quantitative RT-PCR may be performed to monitor BZW1 gene expres - 5. Hearn, T., et al. 2002. Mutation of ALMS1, a large gene with a tandem sion knockdown using RT-PCR Primer: BZW1 (h)-PR: sc-94750-PR (20 µl). repeat encoding 47 amino acids, causes Alström syndrome. Nat. Genet. Annealing temperature for the primers should be 55-60° C and the extension 31: 79-83. temperature should be 68-72° C. 6. Kelsell, D.P., et al. 2005. Mutations in ABCA12 underlie the severe con geni - tal skin disease harlequin ichthyosis. Am. J. Hum. Genet. 76: 794-803. BIOSAFETY Lentiviral particles can be employed in standard Biosafety Level 2 tissue CHROMOSOMAL LOCATION culture facilities (and should be treated with the same level of caution as Genetic locus: BZW1 (human) mapping to 2q33.1. with any other potentially infectious reagent). Lentiviral particles are repli- cation-incompetent and are designed to self-inactivate after transduction PRODUCT and integration of shRNA constructs into genomic DNA of target cells. BZW1 shRNA (h) Lentiviral Particles is a pool of concentrated, transduc - RESEARCH USE tion-ready viral particles containing 2 target-specific constructs that encode 19-25 nt (plus hairpin) shRNA designed to knock down gene expression. Each The purchase of this product conveys to the buyer the nontransferable right vial contains 200 µl frozen stock containing 1.0 x 10 6 infectious units of virus to use the purchased amount of the product and all replicates and derivatives (IFU) in Dulbecco’s Modified Eagle’s Medium with 25 mM HEPES pH 7.3. Suit- for research purposes conducted by the buyer in his laboratory only (whether able for 10-20 transductions. Also see BZW1 siRNA (h): sc-94750 and BZW1 the buyer is an academic or for-profit entity). The buyer cannot sell or other - shRNA Plasmid (h): sc-94750-SH as alternate gene silencing products. wise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party, or otherwise use this product STORAGE or its components or materials made using this product or its components for Commercial Purposes. Store lentiviral particles at -80º C. Stable for at least one year from the date of shipment. Once thawed, particles can be stored at 4º C for up to PROTOCOLS one week. Avoid repeated freeze thaw cycles. See our web site at www.scbt.com or our catalog for detailed protocols and support products. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.
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