Genome-Based Classification of Micromonosporae
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Relative Genetic Diversity of the Rare and Endangered Agave Shawii Ssp
Received: 17 July 2020 | Revised: 9 December 2020 | Accepted: 14 December 2020 DOI: 10.1002/ece3.7172 ORIGINAL RESEARCH Relative genetic diversity of the rare and endangered Agave shawii ssp. shawii and associated soil microbes within a southern California ecological preserve Jeanne P. Vu1 | Miguel F. Vasquez1 | Zuying Feng1 | Keith Lombardo2 | Sora Haagensen1,3 | Goran Bozinovic1,4 1Boz Life Science Research and Teaching Institute, San Diego, CA, USA Abstract 2Southern California Research Learning Shaw's Agave (Agave shawii ssp. shawii) is an endangered maritime succulent growing Center, National Park Services, San Diego, along the coast of California and northern Baja California. The population inhabiting CA, USA 3University of California San Diego Point Loma Peninsula has a complicated history of transplantation without documen- Extended Studies, La Jolla, CA, USA tation. The low effective population size in California prompted agave transplanting 4 Biological Sciences, University of California from the U.S. Naval Base site (NB) to Cabrillo National Monument (CNM). Since 2008, San Diego, La Jolla, CA, USA there are no agave sprouts identified on the CNM site, and concerns have been raised Correspondence about the genetic diversity of this population. We sequenced two barcoding loci, rbcL Goran Bozinovic, Boz Life Science Research and Teaching Institute, 3030 Bunker Hill St, and matK, of 27 individual plants from 5 geographically distinct populations, includ- San Diego CA 92109, USA. ing 12 individuals from California (NB and CNM). Phylogenetic analysis revealed the Emails: [email protected]; gbozinovic@ ucsd.edu three US and two Mexican agave populations are closely related and have similar ge- netic variation at the two barcoding regions, suggesting the Point Loma agave popu- Funding information National Park Services (NPS) Pacific West lation is not clonal. -
Corynebacterium Sp.|NML98-0116
1 Limnochorda_pilosa~GCF_001544015.1@NZ_AP014924=Bacteria-Firmicutes-Limnochordia-Limnochordales-Limnochordaceae-Limnochorda-Limnochorda_pilosa 0,9635 Ammonifex_degensii|KC4~GCF_000024605.1@NC_013385=Bacteria-Firmicutes-Clostridia-Thermoanaerobacterales-Thermoanaerobacteraceae-Ammonifex-Ammonifex_degensii 0,985 Symbiobacterium_thermophilum|IAM14863~GCF_000009905.1@NC_006177=Bacteria-Firmicutes-Clostridia-Clostridiales-Symbiobacteriaceae-Symbiobacterium-Symbiobacterium_thermophilum Varibaculum_timonense~GCF_900169515.1@NZ_LT827020=Bacteria-Actinobacteria-Actinobacteria-Actinomycetales-Actinomycetaceae-Varibaculum-Varibaculum_timonense 1 Rubrobacter_aplysinae~GCF_001029505.1@NZ_LEKH01000003=Bacteria-Actinobacteria-Rubrobacteria-Rubrobacterales-Rubrobacteraceae-Rubrobacter-Rubrobacter_aplysinae 0,975 Rubrobacter_xylanophilus|DSM9941~GCF_000014185.1@NC_008148=Bacteria-Actinobacteria-Rubrobacteria-Rubrobacterales-Rubrobacteraceae-Rubrobacter-Rubrobacter_xylanophilus 1 Rubrobacter_radiotolerans~GCF_000661895.1@NZ_CP007514=Bacteria-Actinobacteria-Rubrobacteria-Rubrobacterales-Rubrobacteraceae-Rubrobacter-Rubrobacter_radiotolerans Actinobacteria_bacterium_rbg_16_64_13~GCA_001768675.1@MELN01000053=Bacteria-Actinobacteria-unknown_class-unknown_order-unknown_family-unknown_genus-Actinobacteria_bacterium_rbg_16_64_13 1 Actinobacteria_bacterium_13_2_20cm_68_14~GCA_001914705.1@MNDB01000040=Bacteria-Actinobacteria-unknown_class-unknown_order-unknown_family-unknown_genus-Actinobacteria_bacterium_13_2_20cm_68_14 1 0,9803 Thermoleophilum_album~GCF_900108055.1@NZ_FNWJ01000001=Bacteria-Actinobacteria-Thermoleophilia-Thermoleophilales-Thermoleophilaceae-Thermoleophilum-Thermoleophilum_album -
Development of Bacterial Communities in Biological Soil Crusts Along
1 Development of bacterial communities in biological soil crusts along 2 a revegetation chronosequence in the Tengger Desert, northwest 3 China 4 5 Author names and affiliations: 6 Lichao Liu1, Yubing Liu1, 2 *, Peng Zhang1, Guang Song1, Rong Hui1, Zengru Wang1, Jin Wang1, 2 7 1Shapotou Desert Research & Experiment Station, Northwest Institute of Eco-Environment and Resources, Chinese 8 Academy of Sciences, Lanzhou, 730000, China 9 2Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions of Gansu Province, Northwest Institute 10 of Eco–Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China 11 12 * Corresponding author: Yubing Liu 13 Address: Donggang West Road 320, Lanzhou 730000, P. R. China. 14 Tel: +86 0931 4967202. 15 E-mail address: [email protected] 16 17 Abstract. Knowledge of structure and function of microbial communities in different 18 successional stages of biological soil crusts (BSCs) is still scarce for desert areas. In this study, 19 Illumina MiSeq sequencing was used to assess the composition changes of bacterial communities 20 in different ages of BSCs in the revegetation of Shapotou in the Tengger Desert. The most dominant 21 phyla of bacterial communities shifted with the changed types of BSCs in the successional stages, 22 from Firmicutes in mobile sand and physical crusts to Actinobacteria and Proteobacteria in BSCs, 23 and the most dominant genera shifted from Bacillus, Enterococcus and Lactococcus to 24 RB41_norank and JG34-KF-361_norank. Alpha diversity and quantitative real-time PCR analysis 25 indicated that bacteria richness and abundance reached their highest levels after 15 years of BSC 26 development. -
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Kent Academic Repository Full text document (pdf) Citation for published version Wichner, Dominik and Idris, Hamidah and Houssen, Wael E and McEwan, Andrew R and Bull, Alan T. and Asenjo, Juan A and Goodfellow, Michael and Jaspars, Marcel and Ebel, Rainer and Rateb, Mostafa E (2016) Isolation and anti-HIV-1 integrase activity of lentzeosides A–F from extremotolerant lentzea sp. H45, a strain isolated from a high-altitude Atacama Desert soil. The DOI https://doi.org/10.1038/ja.2016.78 Link to record in KAR https://kar.kent.ac.uk/61946/ Document Version Author's Accepted Manuscript Copyright & reuse Content in the Kent Academic Repository is made available for research purposes. Unless otherwise stated all content is protected by copyright and in the absence of an open licence (eg Creative Commons), permissions for further reuse of content should be sought from the publisher, author or other copyright holder. Versions of research The version in the Kent Academic Repository may differ from the final published version. Users are advised to check http://kar.kent.ac.uk for the status of the paper. Users should always cite the published version of record. Enquiries For any further enquiries regarding the licence status of this document, please contact: [email protected] If you believe this document infringes copyright then please contact the KAR admin team with the take-down information provided at http://kar.kent.ac.uk/contact.html 1 Isolation and Anti-HIV-1 Integrase Activity of Lentzeosides A-F from Extremotolerant 2 Lentzea sp. H45, a strain isolated from a high altitude Atacama Desert soil 3 Running head: Lentzeosides A-F from Extremotolerant Lentzea sp. -
Dissertation Implementing Organic Amendments To
DISSERTATION IMPLEMENTING ORGANIC AMENDMENTS TO ENHANCE MAIZE YIELD, SOIL MOISTURE, AND MICROBIAL NUTRIENT CYCLING IN TEMPERATE AGRICULTURE Submitted by Erika J. Foster Graduate Degree Program in Ecology In partial fulfillment of the requirements For the Degree of Doctor of Philosophy Colorado State University Fort Collins, Colorado Summer 2018 Doctoral Committee: Advisor: M. Francesca Cotrufo Louise Comas Charles Rhoades Matthew D. Wallenstein Copyright by Erika J. Foster 2018 All Rights Reserved i ABSTRACT IMPLEMENTING ORGANIC AMENDMENTS TO ENHANCE MAIZE YIELD, SOIL MOISTURE, AND MICROBIAL NUTRIENT CYCLING IN TEMPERATE AGRICULTURE To sustain agricultural production into the future, management should enhance natural biogeochemical cycling within the soil. Strategies to increase yield while reducing chemical fertilizer inputs and irrigation require robust research and development before widespread implementation. Current innovations in crop production use amendments such as manure and biochar charcoal to increase soil organic matter and improve soil structure, water, and nutrient content. Organic amendments also provide substrate and habitat for soil microorganisms that can play a key role cycling nutrients, improving nutrient availability for crops. Additional plant growth promoting bacteria can be incorporated into the soil as inocula to enhance soil nutrient cycling through mechanisms like phosphorus solubilization. Since microbial inoculation is highly effective under drought conditions, this technique pairs well in agricultural systems using limited irrigation to save water, particularly in semi-arid regions where climate change and population growth exacerbate water scarcity. The research in this dissertation examines synergistic techniques to reduce irrigation inputs, while building soil organic matter, and promoting natural microbial function to increase crop available nutrients. The research was conducted on conventional irrigated maize systems at the Agricultural Research Development and Education Center north of Fort Collins, CO. -
Supporting Information
Supporting Information Fujimura et al. 10.1073/pnas.1310750111 SI Materials and Methods respectively. Photomicrographs were captured using a Zeiss Axio House Dust Collection. Dust from homes with or without dogs was Imager Z1 and AxioVision 4.8 software (Zeiss). collected using a sterile fabric filter sock inserted into a sterile vacuum nozzle immediately before vacuuming a 3′ × 3′ area for mRNA Extraction, Reverse Transcription, and RT-PCR. mRNA was 3 min. The sock was removed from the vacuum, the collected isolated from ground lung tissue using TRIzol reagent (Invi- μ trogen) or the RNeasy Mini kit (Qiagen) according to manu- dust weighed and sieved through a 300- m sieve to remove large ’ μ debris from the sample. Comparable sieved samples have pre- facturer s instructions. A total of 5 g of RNA per sample was reverse transcribed using murine leukemia virus RTase (Applied viously been used successfully to profile microbial communities Biosystems). Expression of relevant genes was analyzed with present in house dust (1, 2). Dust samples were subsequently TaqMan gene expression assays (Applied Biosystems) using an divided into 25- or 6.25-mg fractions for dog (D)- or no-pet ABI Prism 7500 Sequence Detection System (Applied Bio- (NP)-associated houses, respectively, each stored in a sterile − systems). Gene expression was normalized to GAPDH and ex- 5-mL tube at 20 °C until used for murine supplementation. pressed as fold change over expression in control mice. Murine Models. Cockroach allergen model. BALB/c were purchased Culture and Stimulation of Lymph Node Cells. Mediastinal lymph from The Jackson Laboratory. -
The Degradative Capabilities of New Amycolatopsis Isolates on Polylactic Acid
microorganisms Article The Degradative Capabilities of New Amycolatopsis Isolates on Polylactic Acid Francesca Decorosi 1,2, Maria Luna Exana 1,2, Francesco Pini 1,2, Alessandra Adessi 1 , Anna Messini 1, Luciana Giovannetti 1,2 and Carlo Viti 1,2,* 1 Department of Agriculture, Food, Environment and Forestry (DAGRI)—University of Florence, Piazzale delle Cascine 18, I50144 Florence, Italy; francesca.decorosi@unifi.it (F.D.); [email protected] (M.L.E.); francesco.pini@unifi.it (F.P.); alessandra.adessi@unifi.it (A.A.); anna.messini@unifi.it (A.M.); luciana.giovannetti@unifi.it (L.G.) 2 Genexpress Laboratory, Department of Agriculture, Food, Environment and Forestry (DAGRI)—University of Florence, Via della Lastruccia 14, I50019 Sesto Fiorentino, Italy * Correspondence: carlo.viti@unifi.it; Tel.: +39-05-5457-3224 Received: 15 October 2019; Accepted: 18 November 2019; Published: 20 November 2019 Abstract: Polylactic acid (PLA), a bioplastic synthesized from lactic acid, has a broad range of applications owing to its excellent proprieties such as a high melting point, good mechanical strength, transparency, and ease of fabrication. However, the safe disposal of PLA is an emerging environmental problem: it resists microbial attack in environmental conditions, and the frequency of PLA-degrading microorganisms in soil is very low. To date, a limited number of PLA-degrading bacteria have been isolated, and most are actinomycetes. In this work, a method for the selection of rare actinomycetes with extracellular proteolytic activity was established, and the technique was used to isolate four mesophilic actinomycetes with the ability to degrade emulsified PLA in agar plates. All four strains—designated SO1.1, SO1.2, SNC, and SST—belong to the genus Amycolatopsis. -
Genomic and Phylogenomic Insights Into the Family Streptomycetaceae Lead to Proposal of Charcoactinosporaceae Fam. Nov. and 8 No
bioRxiv preprint doi: https://doi.org/10.1101/2020.07.08.193797; this version posted July 8, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Genomic and phylogenomic insights into the family Streptomycetaceae 2 lead to proposal of Charcoactinosporaceae fam. nov. and 8 novel genera 3 with emended descriptions of Streptomyces calvus 4 Munusamy Madhaiyan1, †, * Venkatakrishnan Sivaraj Saravanan2, † Wah-Seng See-Too3, † 5 1Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 6 Singapore 117604; 2Department of Microbiology, Indira Gandhi College of Arts and Science, 7 Kathirkamam 605009, Pondicherry, India; 3Division of Genetics and Molecular Biology, 8 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 9 Malaysia 10 *Corresponding author: Temasek Life Sciences Laboratory, 1 Research Link, National 11 University of Singapore, Singapore 117604; E-mail: [email protected] 12 †All these authors have contributed equally to this work 13 Abstract 14 Streptomycetaceae is one of the oldest families within phylum Actinobacteria and it is large and 15 diverse in terms of number of described taxa. The members of the family are known for their 16 ability to produce medically important secondary metabolites and antibiotics. In this study, 17 strains showing low 16S rRNA gene similarity (<97.3 %) with other members of 18 Streptomycetaceae were identified and subjected to phylogenomic analysis using 33 orthologous 19 gene clusters (OGC) for accurate taxonomic reassignment resulted in identification of eight 20 distinct and deeply branching clades, further average amino acid identity (AAI) analysis showed 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.07.08.193797; this version posted July 8, 2020. -
Wedding Higher Taxonomic Ranks with Metabolic Signatures Coded in Prokaryotic Genomes
Wedding higher taxonomic ranks with metabolic signatures coded in prokaryotic genomes Gregorio Iraola*, Hugo Naya* Corresponding authors: E-mail: [email protected], [email protected] This PDF file includes: Supplementary Table 1 Supplementary Figures 1 to 4 Supplementary Methods SUPPLEMENTARY TABLES Supplementary Tab. 1 Supplementary Tab. 1. Full prediction for the set of 108 external genomes used as test. genome domain phylum class order family genus prediction alphaproteobacterium_LFTY0 Bacteria Proteobacteria Alphaproteobacteria Rhodobacterales Rhodobacteraceae Unknown candidatus_nasuia_deltocephalinicola_PUNC_CP013211 Bacteria Proteobacteria Gammaproteobacteria Unknown Unknown Unknown candidatus_sulcia_muelleri_PUNC_CP013212 Bacteria Bacteroidetes Flavobacteriia Flavobacteriales NA Candidatus Sulcia deinococcus_grandis_ATCC43672_BCMS0 Bacteria Deinococcus-Thermus Deinococci Deinococcales Deinococcaceae Deinococcus devosia_sp_H5989_CP011300 Bacteria Proteobacteria Unknown Unknown Unknown Unknown micromonospora_RV43_LEKG0 Bacteria Actinobacteria Actinobacteria Micromonosporales Micromonosporaceae Micromonospora nitrosomonas_communis_Nm2_CP011451 Bacteria Proteobacteria Betaproteobacteria Nitrosomonadales Nitrosomonadaceae Unknown nocardia_seriolae_U1_BBYQ0 Bacteria Actinobacteria Actinobacteria Corynebacteriales Nocardiaceae Nocardia nocardiopsis_RV163_LEKI01 Bacteria Actinobacteria Actinobacteria Streptosporangiales Nocardiopsaceae Nocardiopsis oscillatoriales_cyanobacterium_MTP1_LNAA0 Bacteria Cyanobacteria NA Oscillatoriales -
Genome Sequencing and Description of Oerskovia Enterophila Vjag, an Agar- and Cellulose-Degrading Bacterium Vanessa Jag1*† , Anja Poehlein2†, Frank R
Jag et al. Standards in Genomic Sciences (2017) 12:30 DOI 10.1186/s40793-017-0244-4 SHORT GENOME REPORT Open Access Genome sequencing and description of Oerskovia enterophila VJag, an agar- and cellulose-degrading bacterium Vanessa Jag1*† , Anja Poehlein2†, Frank R. Bengelsdorf1, Rolf Daniel2 and Peter Dürre1 Abstract A nonmotile, Gram-positive bacterium that shows an elongated and branching cell shape was isolated from soil samples from the botanical garden of Ulm University, Ulm, Germany. Here, the isolation procedure, identification, genome sequencing and metabolic features of the strain are described. Phylogenetic analysis allowed to identify the isolated strain as Oerskovia enterophila. The genus Oerskovia belongs to the family Cellulomonadaceae within the order Actinomycetales. The length of cells of O. enterophila ranges from 1 μmto15μm, depending on the growth phase. In the exponential growth phase, cells show an elongated and branching shape, whereas cells break up to round or coccoid elements in the stationary growth phase. The 4,535,074 bp long genome consists of 85 contigs with 3918 protein-coding genes and 57 RNA genes. The isolated strain was shown to degrade numerous complex carbon sources such as cellulose, chitin, and starch, which can be found ubiquitously in nature. Moreover, analysis of the genomic sequence revealed the genetic potential to degrade these compounds. Keywords: Oerskovia, Cellulomonadaceae, Cellulose degradation, Soil bacteria, Phylogenetic analysis Introduction from 51% to more than 70% [5–7]. Actinobacteria are Oerskovia enterophila was formerly characterized as widely distributed in terrestrial as well as in aquatic Promicromonospora enterophila by Jàger et al. in 1983 habitats [8, 9]. -
The Diversity of Cultivable Hydrocarbon-Degrading Bacteria Isolated from Crude Oil Contaminated Soil and Sludge from Arzew Refinery in Algeria
THE DIVERSITY OF CULTIVABLE HYDROCARBON-DEGRADING BACTERIA ISOLATED FROM CRUDE OIL CONTAMINATED SOIL AND SLUDGE FROM ARZEW REFINERY IN ALGERIA Sonia SEKKOUR1*, Abdelkader BEKKI1, Zoulikha BOUCHIBA1, Timothy M. Vogel2, Elisabeth NAVARRO2 Address(es): Ing. Sonia SEKKOUR PhD., 1Université Ahmed Benbella, Faculté des sciences de la nature et de la vie, Département de Biotechnologie, Laboratoire de biotechnologie des rhizobiums et amélioration des plantes, 31000 Oran, Algérie. 2Environmental Microbial Genomics Group, Laboratoire Ampère, Centre National de la Recherche Scientifique, UMR5005, Institut National de la Recherche Agronomique, USC1407, Ecole Centrale de Lyon, Université de Lyon, Ecully, France. *Corresponding author: [email protected] doi: 10.15414/jmbfs.2019.9.1.70-77 ARTICLE INFO ABSTRACT Received 27. 3. 2018 The use of autochtonious bacterial strains is a valuable bioremediation strategy for cleaning the environment from hydrocarbon Revised 19. 2. 2019 pollutants. The isolation, selection and identification of hydrocarbon-degrading bacteria is therefore crucial for obtaining the most Accepted 14. 3. 2019 promising strains for decontaminate a specific site. In this study, two different media, a minimal medium supplemented with petroleum Published 1. 8. 2019 and with oil refinery sludge as sole carbon source, were used for the isolation of native hydrocarbon-degrading bacterial strains from crude oil contaminated soils and oil refinery sludges which allowed isolation of fifty-eight strains.The evalution of diversity of twenty- two bacterials isolates reveled a dominance of the phylum Proteobacteria (20/22 strains), with a unique class of Alphaproteobacteria, Regular article the two remaining strains belong to the phylum Actinobacteria. Partial 16S rRNA gene sequencing performed on isolates showed high level of identity with known sequences. -
Marine Rare Actinomycetes: a Promising Source of Structurally Diverse and Unique Novel Natural Products
Review Marine Rare Actinomycetes: A Promising Source of Structurally Diverse and Unique Novel Natural Products Ramesh Subramani 1 and Detmer Sipkema 2,* 1 School of Biological and Chemical Sciences, Faculty of Science, Technology & Environment, The University of the South Pacific, Laucala Campus, Private Mail Bag, Suva, Republic of Fiji; [email protected] 2 Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands * Correspondence: [email protected]; Tel.: +31-317-483113 Received: 7 March 2019; Accepted: 23 April 2019; Published: 26 April 2019 Abstract: Rare actinomycetes are prolific in the marine environment; however, knowledge about their diversity, distribution and biochemistry is limited. Marine rare actinomycetes represent a rather untapped source of chemically diverse secondary metabolites and novel bioactive compounds. In this review, we aim to summarize the present knowledge on the isolation, diversity, distribution and natural product discovery of marine rare actinomycetes reported from mid-2013 to 2017. A total of 97 new species, representing 9 novel genera and belonging to 27 families of marine rare actinomycetes have been reported, with the highest numbers of novel isolates from the families Pseudonocardiaceae, Demequinaceae, Micromonosporaceae and Nocardioidaceae. Additionally, this study reviewed 167 new bioactive compounds produced by 58 different rare actinomycete species representing 24 genera. Most of the compounds produced by the marine rare actinomycetes present antibacterial, antifungal, antiparasitic, anticancer or antimalarial activities. The highest numbers of natural products were derived from the genera Nocardiopsis, Micromonospora, Salinispora and Pseudonocardia. Members of the genus Micromonospora were revealed to be the richest source of chemically diverse and unique bioactive natural products.