Polyclonal Antibody to Guanylate Cyclase Soluble GUCY1A3 - Aff - Purified

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AP20764PU-N Polyclonal Antibody to Guanylate cyclase soluble GUCY1A3 - Aff - Purified

Alternate names: GCS-alpha-1, GCS-alpha-3, GUC1A3, GUCSA3, GUCY1A1, Guanylate cyclase soluble subunit alpha-3, Soluble guanylate cyclase large subunit, sGC alpha Quantity: 0.1 mg Concentration: 1.0 mg/ml Background: Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase, a soluble form (GCS or sGC), which act as receptors for nitric oxide and a membrane-bound receptor form (GC), which are peptide hormone receptors. The GC-C protein is composed of an extracellular domain, a single transmembrane domain, and a cytoplasmic region consisting of a kinase-like domain and a catalytic domain. It is expressed as two differentially glycosylated forms, a 130 kDa precursor form present in the endoplasmic reticulum and a 145 kDa form present on the plasma membrane. Ligand binding to the extracellular domain of GC-C promotes the accumulation of cGMP. GC-C acts as the receptor for heatstable enterotoxins, small peptides secreted by some pathogenic strains of E. coli that cause severe secretory diarrhea. GC-C also binds to guanylin and uroguanylin peptides, which modulate renal function in response to oral salt load. Uniprot ID: Q02108 NCBI: NP_000847 GeneID: 2982 Host: Rabbit Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human GCS-α-1. Format: State: Liquid purified Ig fraction (> 95% by SDS-PAGE) Purification: Affinity-chromatography using epitope-specific immunogen Buffer System: Phosphate buffered saline (PBS), pH~7.2 Preservatives: 15 mM Sodium Azide Applications: Western blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Molecular Weight: ~ 77 kDa Specificity: This antibody detects endogenous levels of GCS-alpha-1 protein. (region surrounding Asp423) Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse and Rat. Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20170911 1 / 3 AP20764PU-N: Polyclonal Antibody to Guanylate cyclase soluble GUCY1A3 - Aff - Purified

Pictures: Western blot analysis of GCS-alpha-1 antibody (Cat.-No. AP20764PU-N) in extracts from THP1 cells.

Immunohistochemistry analysis of GCS- alpha-1 antibody (Cat.-No.- AP20764PU- N) in paraffin-embedded human lung carcinoma tissue.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20170911 2 / 3 AP20764PU-N: Polyclonal Antibody to Guanylate cyclase soluble GUCY1A3 - Aff - Purified

Western blot analysis of GCS-alpha-1 antibody (Cat.-No. AP20764PU-N) at 1/500 dilution. Lane 1: HEK293T whole cell lysate. Lane 2: sp2/0 whole cell lysate. Lane 3: H9C2 whole cell lysate.

Immunohistochemistry analysis of GCS- alpha-1 antibody (Cat.-No.- AP20764PU- N) in paraffin-embedded human breast carcinoma tissue at 1/100.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20170911 3 / 3