Antibody Cat. No.: 16-846

Complement Factor B Antibody

Immunohistochemistry of paraffin-embedded human mammary cancer using Immunohistochemistry of paraffin-embedded mouse lung using Complement Factor B Complement Factor B antibody (16-846) at dilution of 1:200 (40x lens). antibody (16-846) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of Raw264.7 cells using Complement Factor B Polyclonal Antibody (16-846) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

September 29, 2021 1 https://www.prosci-inc.com/complement-factor-b-antibody-16-846.html Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Recombinant fusion containing a sequence corresponding to amino acids 80-420 IMMUNOGEN: of human Complement Factor B (NP_001701.2).

TESTED APPLICATIONS: IF, IHC, IP, WB

WB: ,1:500 - 1:2000

IHC: ,1:50 - 1:200 APPLICATIONS: IF: ,1:50 - 1:200

IP: ,1:20 - 1:50

POSITIVE CONTROL: 1) HT-29

2) K-562

3) 293T

4) A-549

5) HepG2

6) Mouse liver

PREDICTED MOLECULAR Observed: 86kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: CFB

September 29, 2021 2 https://www.prosci-inc.com/complement-factor-b-antibody-16-846.html Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, GBG, PBF2, ALTERNATE NAMES: factor B, Complement factor B Ba fragment, Complement factor B Bb fragment, CFB, BF, BFD

GENE ID: 629

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

This encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with to form the alternative pathway C3 convertase. Bb is BACKGROUND: involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on 6. This cluster includes several involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age- related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.

ANTIBODIES FOR RESEARCH USE ONLY.

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