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Study of Physico-Chemical and Sensory Properties of Red Wines from Black Grapes (Vitis Vinifera L.) in Different Areas of Albania

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Study of Physico-Chemical and Sensory Properties of Red Wines from Black Grapes (Vitis Vinifera L.) in Different Areas of Albania Journal of Hygienic Engineering and Design Original scientific paper UDC 663.222:663.251(496.5) STUDY OF PHYSICO-CHEMICAL AND SENSORY PROPERTIES OF RED WINES FROM BLACK GRAPES (VITIS VINIFERA L.) IN DIFFERENT AREAS OF ALBANIA Anisa Dhroso1*, Hasime Manaj1, Elena Muca1, Rozana Troja1, Ilirjan Malollari1 1Department of Industrial Chemistry, Faculty of Natural Sciences, University of Tirana, Boulevard “Zogu I”, 1001Tirana, Albania *e-mail: [email protected] Abstract Grape vine is the most commonly grown kind of fruit titration of the standard solution of iodine, SSH 1446- worldwide. Albania belongs chronologically to the old 7:1987 and SSH 1446-6:1987 respectively. world of wine producing countries. Many factors such The content of alcohol in the red wine ranges between as: variety, soil, climate, growing conditions and wine- 10 and 13% vol. Wines with alcohol content from 12 making techniques influence the fruit composition to 14% vol are marked as strong. Total acidity values and therefore, the style of wine that can be produced. were observed at about 5 to 7 g/L tartaric acid range. Wine is an alcoholic beverage produced through the The values of pH ranging between 3 and 4 were con- partial or total fermentation of grapes. Wine is charac- sidered as optimal for red wine. Sulphur dioxide affect terized by color: white, rose and red. Albania can be human health, hence the maximum limit for red wine separated into four wine regions, which are mainly de- 180 mg/L must not be exceeded. The values of total fined by their altitude. Varieties from theVitis vinifera sulphur did not exceed the maximum limit. Phenolic group are most widely used for winemaking. Wine can composition of the finished wines depends on the be classified by human experts or by physicochemical grape and winemaking practices, and polyphenol in- laboratory experiments. Physicochemical and sensory dex ranges from 60 to 90. analyses of wine are important in establishing their quality and authenticity. The aim of the study was to Physicochemical and sensory parameters of red wines investigate physico-chemical and sensory properties can be influenced by the plant’s environment, differ- between red wines from black grapes (Vitisvinifera ent areas, varieties, viticulture and enological practic- L.) produced in different regions of Albania (Tirana, esand can be used as a way of characterizing the wine Durres, Berat, Korca). quality. The values of physicochemical and sensory pa- rameters in red wine produced in different regions of The sensory properties like: colour, taste and aroma Albania were in accordance to the national law of the were first examined. Shade and intensity of wine co- Albanian Food Law and EU Food legislation. lour were calculated using optical methods. Then, dif- ferent physicochemical parameters were determined: dry matter, ash, density, total acidity, and volatile acid- Key words: Red wine, Physicochemical, Sensory, Quality. ity, alcohol content, reduced sugars, free and total SO2, pH and polyphenol index following the analytical methods described in Albanian standard. The con tent 1. Introduction of alcohol in wine was determined by SSH 1446-1:1987 method [19]. The concentration of reduced sugars was Albania belongs chronologically to the old world of determined with Fehling method SSH 1446-2:1987. wine producing countries. The ancient Roman writ- Total and free acidity were determined by analyti- er Elder described Illyrian wine as being very sweet or cal methods according to, SSH 1446-3:1987, and SSH luscious and refers to it as taking the third rank among all the wines [8]. Albania is a mountains Mediterra- 1446-4:1987. Free and total SO2 were determined by 48 Journal of Hygienic Engineering and Design nean country and extends with the Mediterranean wines were calculated using optical methods. Physi- Sea in the west. Favorable climate and fertile soil of cally, the color is a light characteristic, measurable in the mountainous areas of the country are well suited terms of intensity and wavelength. The intensity and to viticulture. The wine production is associated main- hue of color in tested red wines were determined spec- ly with countries of moderate climate with long, hot trophotometrically by measuring the absorbance at: summers. However, the vineyards are located also in 420, 520 and 620 nm [11]. countries of cooler climate [10, 17]. The country can Polyphenol index was determined by measuring the be separated into four wine regions, which are main- absorbance at 280 nm [12]. For spectrophotometric ly defined by their altitude:Western lowland that ris- measurements, spectrophotometer UV-2100C was es to 300 m (Tirana, Durresi, Shkodra, Lezha, Lushnja, used. Fier, Vlora, and Delvina); Central hilly region which varies between 300 and 600 m altitude (Elbasan, Kruje, Then different physicochemical parameters for each Gramsh, Berat, Permet, Librazhd, and Mirdita); Eastern wine were determined: dry matter, ash, density, total sub-mountainous region which lies between 600 and acidity, and volatile acidity, alcohol content, reduced 800 m (Pogradec, Korca, Leskovic, and Peshkopi); and sugars, free and total SO2, pH, and polyphenol index (4) Mountains wines are also grown as high as 1,000 following the analytical methods described in Alba- m [6]. Many factors such as: variety, soil, climate, grow- nian standard [19]. Tests were performed in triplicate ing conditions and winemaking techniques influence for each sample. For the determination of the density, the fruit composition and therefore, the style of wine the pycnometric method was used. For this purpose, that can be produced. Varieties from the Vinifera group pycnometers with a volume of 50 mL and an analytical are most widely used for winemaking. Approximately balance with an accuracy of 0.0001 g were used. Based 5,000 types’ of varieties are recognized, but only fifteen on the determination of the density of wine and dis- types of grape-varieties are able to produce excellent tillate, the extract was determined (g/L). Ash content wines (Cabernet sauvignon, Merlot, Pinot noir). Wine is of wines was determined by gravimetric method. Con- an alcoholic beverage produced through the partial or tent of alcohol in wine was determined by SSH 1446- total fermentation of grapes. Fermentation processes 1:1987 method [19]. The pH measurement of red wine are done by the yeast Saccharomyces, whereby the sug- was obtained with a pH meter (PHS-3CW micropro- ars in the fruit juice are metabolized into alcohol and cessor pH meter) was calibrated with standard solu- dioxide carbon, that later react to form organic acid, tions buffered. Total and free acidity were determined aldehydes, esters and other chemical components [1, by analytical methods according to, SSH 1446-3:1987 3]. Wine is characterized by the following colors: white, [21], and SSH 1446-4:1987 [22]. All titrated acids in the rose and red. Wine can be classified by human experts wine are the sum of compounds titratable by standard or by physicochemical laboratory experiments. Physi- alkaline solution to pH 7. Carbonic acid is not includ- cochemical and sensory analyses of wine are import- ed in total acidity. The amount of acetic acid in wine, ant in establishing their quality and authenticity. Eval- which at too high of levels can lead to an unpleasant, uation prevents illegal adulteration and assures quality vinegar taste. Sweetness of the wine is determined by for the wine market [18]. the amount of residual sugar in the wine after fermen- tation, relative to the acidity present in the wine. Con- The aim of the study was to investigate physico-chemical centration of reduced sugars was determined with Fe- and sensory properties between red wines from black hling method SSH 1446-2:1987 [20]. Free and total SO grapes (Vitis vinifera L.) produced in different regions of 2 were determined by titration of the standard solution Albania (Tirana, Durres, Berat, Korca, and Librazhd). of iodine, SSH 1446-7:1987 [23], and SSH 1446-6:1987 [24] respectively. Free form of SO2 prevents microbial growth and the oxidation of wine. 2. Materials and Methods Red wines from black grapes (Vitis vinifera L.) available in Albanian market were characterized for physico- 3. Results and Discussion chemical and sensory properties produced in different The red wine quality and behavior can be influenced regions of Albania (Tirana, Durres, Berat, and Korca). by the plant’s environment, species and varieties, viti- Following red wines were purchased: No. 1 - Cabernet culture and enological practices [20]. Several physico- Souvignon from Librazhd; No. 2 - Merlot from Korca; chemical and sensory properties of red wines were in- No. 3 - Shesh i zi from Durres; No. 4 - Mixed varieties vestigated. We started the wine analysis with the sen- Cabernet Souvignon, Shesh i Zi and Merlot from Berat; sory properties like color, and taste and aroma were and No. 5 - Mixed varieties Merlot and Cabernet Sauvi- first examined. The red color in brownish shades of all gnon from Tirana. samples was observed. Wines produced form different We started wine analysis with the sensory properties varieties were rich with flavor: light oak wood flavor - like color, taste and aroma. Color intensity and hue of No. 1; and dry fruit flavor - No. 2. All the samples were 49 Journal of Hygienic Engineering and Design with fruit taste: apple taste - No. 1; dried plums - No. 2; Ethyl alcohol is the predominant constituent of alco- blue plum, black berries - No. 3. No. 4 and No. 5 sam- holic fermentation. Variation in the alcohol content ples, mixed different varieties were observed that Cab- of wine in different varieties of grape might be due to ernet Sauvignon taste and Merlot flavor prevails. the variation in amounts of sugar present in berries [7]. Con tent of alcohol in wine is affected by many factors, Color intensity and polyphenol index are presented in such as ripeness of the grapes at the time of harvest, Figures 1 and 2.
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