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Track 3 – Disease and Treatment T3:Po.01 T3:Po.02 International Journal of Obesity (2007) 31, S106–S174 & 2007 Nature Publishing Group All rights reserved 0307-0565/07 $30.00 www.nature.com/ijo POSTER SESSION: TRACK 3 – DISEASE AND TREATMENT T3:PO.01 T3:PO.02 N-Terminal pro-B-type natriuretic peptide and echocardiographic abnormalities in Presenting a new model for weight control severely obese patients: correlation with visceral fat 1 Corsi Massimiliano Marco1, Marocchi Alessandro2, Ambrosi Bruno3, Malavazos Movahedi Ahmadreza Alexis Elias3 1University, Isfahan, Iran 1Institute of General Pathology, University of Milan; 2Dept of Laboratory Medicine, Niguarda Hospital; 3Endocrinology Unit, IRCCS Policlinico San Donato Hospital, The purpose of this study was to establish a metabolic dietary program University of Milan for weight loss or gain in which hypothalamus may not be stimulated in decline or increase Basal Metabolic Rate.18 healthy male and female (25 Many studies have confirmed NT-proBNP as a sensitive marker for left ventricular (LV) hypertrophy and/or asymptomatic left ventricular dysfunction and it is to 50 years old) were assigned to three groups. Group A experienced particularly reliable because of its high negative predictive value To our weight loss metabolic program, they decreased 10% of their normal daily knowledge, recent findings on the relation between NT-proBNP and morphologic food amount for three days (action phase) and returned to their normal and dynamic cardiac abnormalities in obesity are still inconsistent and daily food amount for one day (return phase). They observed it for controversial. We measured NT-proBNP concentrations in 27 severely obese twelve days. The decrease for the remained each twelve -day period was women with no complications and 15 normal-weight patients. All subjects were 15%, 20%, 25%, 30%, for the action phases and 5%, 5%, 10%, 10%, for young with normal renal function, pre-menopausal, normotensive, normoglycemic, the changing return Phases alternatively. The program went on so that at not dyspneic, drug-free women; thus excluding the possibility that high NT- proBNP concentrations may be associated with hypertension and diabetes. All the last twelve -day period the food amount decrease for action phases patients and controls underwent echocardiography. Visceral adipose tissue (VAT) was 30% and for the changing return phases was 10%.Group B followed and subcutaneous adipose tissue (SAT) areas in the obese group were assessed by weight gain metabolic program just like the program for weight loss computed tomography. Serum NT-proBNP concentrations were measured using group except that they increased the percents instead of decrease. Group the proBNP (Roche) assay on an Elecsys 2010 analyzer (Roche). Obese women C was as control group. The total period for the experimental and control had higher mean NT-proBNP concentrations than controls (P<0.001). NT-proBNP 2 groups was 60 days. To analyze data T test was used. Results: Group A concentrations were correlated with the VAT area (r =0.60, P<0.0001), VAT/SAT decreased their body weight from 86.5 kg to 79.33 kg (sig =.001). (r2=0.38, P<0.001) but not with SAT (P= -0.61). Fifteen obese patients with a greater amount of visceral fat (VAT >130 cm2) had higher mean NT-proBNP Group B gained 2.16 kg (sig = .003). No significant change in body concentrations than those with VAT less than 130 cm2 (n= 12) (P<0.02). The most weight was seen in control group (sig =.822 ). novel finding in our study is the significant correlation between NT-proBNP and echocardiographic parameters in an homogeneous population, even after correction for increased VAT (P<0.05). This may indicate that NT-proBNP itself could serve as an indicator of LV morpho-functional changes. T3:PO.03 T3:PO.04 Effect of weight loss on plasma lipids and glucose in patients with type 2 Effect of exercise training on plasma eotaxin levels diabetes Kim Seon Mee1, Choi Kung Mook2, Han Jee Hye3, Park Hye Soon4 Faghih Shiva1, Ansari Negin1 1Department of Family Medicine, Korea University Medical Center, 1Islamic Azad University-Ahvaz Branch, Tehran, Iran Korea; 2Division of Endocrinology and Metabolism, Department of Internal Medicine, Korea University Medical Center, Korea; Objective: To determine the effect of weight loss on plasma triglyceride, 3Department of Family Medicine, Eulji University School of Medicine, cholesterol and fasting plasma glucose in obese patients with type 2 Korea; 4Department of Family Medicine, University of Ulsan College of diabetes. Methods: A total of 68 free-living obese patients with type 2 Medicine, Korea diabetes were randomized to either a low calorie diet or a normal diet groups. FPG, Tg, cholesterol, weight and height of subjects were Context: Recent study revealed that eotaxin, which is an important measured before the intervention and after one month. chemokine in extrinsic asthma, is associated with obesity in mice and Results: Mean ± SD of weight, BMI, Tg, cholesterol, and FPG of LCD humans. Exercise training exerts beneficial effects on metabolic and group were 98.97 ± 18.5(Kg), 35.42 ± 6.20(), 236.88 ± 65.21(), 240.97 ± vascular risk factors in obese patients. 63.13(), and 215.08 ± 72.39() respectively, which fell to 94.80 ± Objectives: We have investigated whether exercise can alter the plasma 17.02(Kg), 33.94 ± 5.80 (), 176.11 ± 45.50(), 176.22 ± 29.77(), and eotaxin levels in non-diabetic subjects. 129.37 ± 41.61() after a month (P < 0.001 for all).No significant Design and Patients: In a prospective study, forty-seven Korean women different was seen in control group. In this study there were significant (age 47.1 ± 6.5, body mass index (BMI) 27.0 ± 2.9 kg/m2) were correlations between rate of weight loss and Tg (P< 0.001, r = 0.52) and included. Fating plasma eotaxin levels and metabolic parameters were cholesterol (P<0.01, r = 0.45) reduction. measured in a supervised aerobic exercise program for eight weeks. Conclusion: Even small amount of weight loss can lead to a significant Results: At baseline, plasma eotaxin concentrations were elevated in reduction in serum Tg, cholesterol and glucose. So we can use it as a subjects with central obesity (waist circumference < 80 cm, 73.6 ± 17.8 suitable treatment to reduce the risk of coronary-heart diseases. pg/ml) compared to those without central-obesity (waist circumference < 80 cm, 64.2 ± 4.2 pg/ml, P = 0.005). In multiple regression analysis, eotaxin levels were independently associated with waistto- hip ratio and body weight (R2 = 0.330). After exercise program, eotaxin levels were significantly decreased from 72.0 ± 16.7 to 66.9 ± 14.3 pg/ml (P = 0.038) without change of insulin resistance. Conclusions: Regular exercise training with weight loss induced a reduction of plasma eotaxin levels. Abstracts S107 T3:PO.05 T3:PO.06 Effects of 6 months of exercice and isoflavones on cardiovascular disease risk Lupinus angustifolius (sweet lupin) seed protein - an anti-obesity protein found factors in obese postmenopausal women 1 Rahman Mohammed 1 Aubertin-Leheudre Mylene 1Bangladesh Agricultural University 1Research Center on Aging, University of Sherbrooke The effects of raw sweet lupin (Lupinus angustifolius) meal on the growth and N utilization of rats were determined from five N balance experiments. Sweet lupin seed Objective: To investigate if 6 months of exercise with isoflavones could grown in Western Australia, obtained as meal, either unsupplemented (LMU) or fully supplemented with required amino acids (360 g kg-1)(LMFS), was tested. Rats fed improve clinical risk factors that predispose to CVD in obese postmenopausal -1 lactalbumin (130 g kg )(LACT) were used as positive controls, while rats fed a women. nonprotein diet (NPC) were used as negative controls. In addition, seed protein, extracted Design: Randomised double blind controlled trial. at pH 7.0 with water and insoluble after dialysis at pH 7.0 against distilled water (LPADI; Subjects: Fifty healthy obese postmenopausal women were divided in 2 124 g kg-1), was also used. Details of chemical analyses were carried out and found that groups and submitted during 1 year on a supplementation (25 in isoflavones LPADI is the storage protein of lupin seed. Sodium dodecyl sulphate polyacrylamide gel (ISO) and 25 in placebo (PLA)) and followed during the last 6 months an electrophoresis (SDS-PAGE had shown that LPADI contains three polypeptides with exercise program (3 times by week)) to compare CVD risk factors. molecular weights between 30 and 36 kDa which are similar to lectins obtained from Measurements: Body composition (DXA), resting energy expenditure Phaseolus vulgaris, Abrus precatorius and Ricinus communis. cal analyses The diets contained the same amounts of energy and protein and were supplemented with essential (indirect calorimetry), daily energy expenditure (accelerometry) and dietary amino acids, vitamins and minerals to target requirements for rats. Inclusion of LPADI in intake (3-day dietary record), metabolic profile (blood lipids, fasting insulin, the diet of growing rats caused urinary losses of N, almost all as urea, hypoproteinaemia fasting glucose, SHBG, CRP) at baseline and 12 months. and increase in body water that resulted in the lowest NPU values, N balance and growth Results: We observed a significant effect of the intervention on body weight, rate as compared with other diets used. These rats developed atrophy of the spleen (low total and abdominal FM in kg and %, BMI and gain appendicular FFM, and dry weight) and had a comparatively smaller thymus gland than those given raw meals. It FFM/FM ratio in ISO group but not in PLA group. ISO group presented a is possible that the toxic protein component in the sweet lupin, which has negligible in vitro haemagglutination properties and is extremely toxic in vivo, exerts toxicity by significantly greater level of SHBG compared to PLA group.
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