HCA Research Hans Christian Andersen Children's Hospital

ANNUAL REPORT 2016 HCA Research  Hans Christian Andersen Children’s Hospital  OUH  WELCOME

 HCA RESEARCH

 COLLABORATION

 RESEARCH FOCUS

 PH.D. PROJECTS

 ASSOCIATED PH.D. PROJECTS

 PREGRADUATE PROJECTS

 ODENSE CHILD COHORT CONTENTS  PUBLICATIONS  TEACHING

 NEW ADJUNCT PROFESSOR

 RESEARCH SEMINAR

 GRANTS

 CONTACT

The annual report for HCA Research at the Hans Christian Andersen Children’s Hospital provides a brief snapshot of our activities and contri- butions to pediatric research in 2016. The report presents projects, results, teaching, grants and collaboration and as well as expectations for 2017.

In total, 2016 has been a good year for HCA Research. New projects have been started, a number of projects have been successfully finalized and the number of publications has increased. The research funding pro- file has temporarily decreased, but is expected again to increase in 2017

WELCOME As we move forward we will maintain our strength in interdisciplinary research while seeking to build new partnerships and recruit new researchers in new projects, in order to sustain the excellence and impact of our research unit.

Steffen Husby Professor & Head of Research

HCA Research is both a part of Hans Christian Andersen Chil- dren’s Hospital, Odense University Hospital, and a part of Depart- ment of Clinical Research, Faculty of Health Sciences at University of Southern Denmark. We are a team of professors, consultants, associate professors, ph.d. students, medical students, technici- ans, nurses and secretary assistants.

I S S I O N:

to promote and implement pediatric clini- M cal and experimental research at a high professional level. I S I O N: to optimize health HCA RESEARCH and treatment of children’s illness V through interdisciplinary, clinical and biomedi- cal research of high quality with focus on prevention, di- agnostics and treatment.

HCA Research prioritizes local, regional, national and international collaboration. Our organizational position as a part of both University of Southern Denmark and Odense University Hospital provides us many opportunities to collaborate with other departments and institutes.

In 2016 we have strengthened existing relations and expanded new promising collaborations.

COLLABORATION HCA Research supports research in all pediatric fields. The main theme for research at HCA Research is;

FOCUS OF THE CHILD: DEVELOPMENT, DISEASE, PREVENTION

Gastroentero- Allergy/Asthma logy/Nutrition

Infection Neurology Nephrology

Neonatology

Hematology

Neonatal RESEARCH FOCUS Home Care Rheumatology

Oncology

Immunology Community Pediatrics

Cardiology Endocrinology Congenital abdominal wall defects – Incidence and pregnancy outcome of prenatally diagnosed abdominal wall defects. Peri- and postnatal prognostic predictors for short- and long-term outcome. - Kirsten Risby, M.D., ph.d. student (defended 2016)

Effect of bovine colostrum on toxicity and inflammatory responses during chemotherapeutic treatment of childhood cancer - Mathias Rathe, M.D., ph.d. student (defended 2016)

Bone turnover in relation to glucose metabolism and pubertal development - Maria Rønne, M.D., ph.d. student

Neonatal Home Care supported by Telemedicine - Kristina Garne Holm, cand.cur., ph.d. student

Allergic diseases and sensitization in children up to the age of 14 years. A follow-up investigation of the DARC (Danish Allergy Research Center) Cohort - Elisabeth Søgaard Christiansen, M.D., ph.d. student

Vitamin D deficiency in mature and preterm newborns – A human cohort and animal experimental study - Sine Lykkedegn, M.D., ph.d. student (defended 2016)

Growth, risk of allergy and of metabolic syndrome in 6 year old children born very preterm –influence of nutriti-on from discharge to 4 months of age - Line Hedegaard Toftlund, M.D., ph.d. student

Importance of vitamin D status for obstetric morbidity in Odense Child Cohort and in an animal model, with focus on preeclampsia - Louise Bjørkholt Andersen, M.D., ph.d. student (defended 2016)

Early environmental exposures and risk of coeliac disease - the significance of early gut colonization - Stine Dydensborg Sander, M.D., ph.d. studerende PH.D. PROJECTS Morbus Hirschsprung Disease - Nina Højer Hansen, M.D., ph.d. student

Infections in early childhood – vitamin D status, risk factors and use of antibiotics - Nikolas Christensen, M.D., ph.d. student

Neonatal hypoglycaemia: Prevention, treatment and cerebral sequelae - Annett Helleskov Rasmussen, M.D., ph.d. student

Predicting the natural course of paediatric asthma – a longitudinal study investigating the significance of no -vel prognostic markers - Benjamin Hoffmann-Petersen, M.D., ph.d. student

Surfactant Protein D (SP-D) and Microfibril-Associated Protein 4 (MFAP4) as possible biomarkers for tran- sient early wheezing and bronchial asthma in children - Helene Marlies Rasmussen, M.D., ph.d. student

Breastfeeding, breast milk components, cardiovascular health and growth in early childhood - Signe Bruun, M.D., ph.d. student

Antibody levels in immunocompromised children after vaccination - Lotte Jensen, M.D., ph.d. student, Copenhagen University

AsthmaVent – Effect of Ventilation on Asthma Control in Children with House Dust Mite Allergy and Asthma - Nina Hogaard, M.D., ph.d. student, Aarhus University

Morbidity in children born by women with chronic disease - Line Riis Jølving , M.D., ph.d. student, Syddansk Universitet

NEOCOL - bovine colostrum for preterm born infants - Agnethe May Ahnfeldt, M.D., ph.d. student, Copenhagen University

Medication of children at hospital. An adverse event study. - Rikke Rishøj, M.D., ph.d. student, Dept. of Pharamcology

Congenital hyperinsulinism and early onset diabetes: Hunting genes in genetic disorders influencing insulin secretion and glucose levels using tissue expression microarrays and exome sequencing - AlHaidan, Yazeid, M. MD (Saudi Arabia), M.D., ph.d. student, Faculty of Health Sciences, University of Southern Denmark. Dept. of Clinical Genetics and Hans Christian Andersen Children’s Hospital, Odense University Hospital ASSOCIATED PH.D. PROJECTS Sex-specific patterns in the association between cord 25-hydroxyvitamin D and linear growth in 3-months- to 3-year-old children. An Odense Child Cohort study - Mathilde Egelund Christensen, medical student

Girls only: Cord 25-hydroxyvitamin D associates inversely with blood pressure, but not with body fat. An Oden-se Child Cohort study - Søs Dragsbæk Larsen, medical student

Cord 25-hydroxyvitamin D and infant cranial growth: An Odense Child Cohort Study - Sissil Egge, medical student

Iron deficiency in healthy 18 months-old Danish children. Prevalence and associated factors. A subproject in the Odense Child Cohort - Anh Thao Nguyen Andersen, medical student

Season of birth and risk of infection among children less than five years of age – an Odense Child Cohort study - Karina Christensen

Treatment of Congenital hyperinsulinism - Amalie Greve Rasmussen

Gastrointestinal toxicity and inflammatory responses in doxorubicin treated PREGRADUATE Dmbt1 deficient mice - Andreas Arnholdt Pedersen

Anthracycline Induced Long-Term Effects of the Heart after Treatment for Acute Lymphoblastic Leukemia(ALL) during Childhood - Julia Palmborg

PROJECTS s-25-hydroxyvitamin D and C3-epimers in pregnancy and infancy: An Odense Child Cohort Study - Nanne Dreier Mydtskov The Odense Child Cohort STATUS 2016 is a prospective obser- vational birth cohort with children born in the Municipality of Odense in 2010—2013. Of the eligi- 2.543 ble population of 6,707 children / 2.494 families pregnant women, 4,017 declined while 2,874 (43%) enrolled in the cohort. The children are being examined at the age of 3 and 18 months, 3, 5, 7, 9, 12, 15 and 18 years. 5 years examination (850 children The purpose of the project is to provide in- have participated so far) depth information about the interaction between maternal and child health and the impact of social and environmental factors in order to improve child health and wellbe- 3 years ing. Information is collected from question- examination ﬁnished(1.945 naires, medical record and biological mate- children have participated) rial from parents and child. In addition, information is collected from health visitors, nurseries and school records. 222.641 ODENSE CHILD Odense Child Cohort is a strong collabo- samples in the bio ration between the Municipality of Odense, the University of Southern Denmark, Oden- se University Hospital and the Mental Health Services in the Region of Southern 2.405.000 DKK grant Denmark. It is financially supported by the Municipality of Odense, Odense University COHORT Hospital, Region of Southern Denmark, OPEN (Odense Patient Data Exploratory 15 Network), Mental Health Services in the research projects Region of Southern Denmark, The Health Foundation, The Ronald McDonald Child Foundation and many small grants. 13 publications

Akiki, Zeina; Fakih, Dalia; Jounblat, Rania; Chamat, Soulaima; Waked, Mirna; Holmskov, Uffe; So- rensen, Grith L; Nadif, Rachel; Salameh, Pascale / Surfactant protein D, a clinical biomarker for chronic obstructive pulmonary disease with excellent discriminant values. Molecular Therapy, Vol. 11, Nr. 3, 03.2016, s. 723-730.

Andersen LB, Jørgensen JS, Herse F, Andersen MS, Christesen HT, Dechend R. / The association between angiogenic markers and fetal sex: Implications for preeclampsia research. J Reprod Immu- nol 2016;177:24-29

Andersen LB, Dechend R, Karumanchi SA, Nielsen J, Joergensen JS, Jensen TK, Christesen HT. / Early pregnancy angiogenic markers and spontaneous abortion: an Odense Child Cohort study. Am J Obstet Gynecol 2016;215:594.e1-594.e11

Andersen, Louise Bjørkholt; Golic, Michaela; Przybyl, Lukasz; Sorensen, Grith Lykke; Jørgensen, Jan Stener; Fruekilde, Palle; von Versen-Höynck, Frauke; Herse, Florian; Højskov, Carsten Schri- ver; Dechend, Ralf; Christesen, Henrik Boye Thybo; Haase, Nadine / Vitamin D depletion does not affect key aspects of the preeclamptic phenotype in a transgenic rodent model for preeclampsia. American Society of Hypertension. Journal, Vol. 10, Nr. 7, 2016, s. 597-607.e1.

Andersen, Louise B; Dechend, Ralf; Karumanchi, S Ananth; Nielsen, Jan; Joergensen, Jan S; Jen- sen, Tina K; Christesen, Henrik Boye Thybo / Early pregnancy angiogenic markers and spontaneous abortion : an Odense Child Cohort study. American Journal of Obstetrics & Gynecology, Vol. 215, Nr. 5, 2016, s. 594.e1-594.e11.

Andersen, Louise Bjørkholt; Dechend, Ralf; Jørgensen, Jan Stener; Luef, Birgitte Møller; Nielsen, Jan; Barington, Torben; Christesen, Henrik Boye Thybo / Prediction of preeclampsia with angiogenic biomarkers : Results from the prospective Odense Child Cohort. Hypertension in Pregnancy, Vol. 35, Nr. 3, 2016, s. 405-419.

Backer, Vibeke; Lykkegaard, Jesper; Bodtger, Uffe; Agertoft, Lone; Korshoej, Lene; Braüner, Elvira PUBLICATIONS Vaclavik / The Danish National Database for Asthma. Clinical Epidemiology, Vol. 8, 2016, s. 601- 606.

Boesen, Martin Lehmann; Bygum, Anette; Hertz, Jens Michael; Zachariassen, Gitte / Newborn with severe epidermolysis bullosa : to treat or not to treat? BMJ Case Reports, Vol. 2016, 2016.

Broesby-Olsen, Sigurd; Kristensen, Thomas Kielsgaard; Agertoft, Lone; Møller, Michael Boe; Her- mann, Pernille; Torfing, Trine; Havelund, Troels; Mørtz, Charlotte G; Bindslev-Jensen, Carsten; Vestergaard, Hanne / Mastocytose. Ugeskrift for Laeger, Vol. 178, Nr. 15, V10150854, 15.02.2016, s. 2-6.

Broesby-Olsen, Sigurd; Dybedal, Ingunn; Gülen, Theo; Kielsgaard Kristensen, Thomas; Boe Møller, Michael; Ackermann, Leena; Sääf, Maria; Karlsson, Maria A; Agertoft, Lone; Brixen, Kim; Hermann, Pernille; Stylianou, Eva; Mortz, Charlotte G; Torfing, Trine; Havelund, Troels; Sander, Birgitta; Berg- ström, Anna; Bendix, Marie; Garvey, Lene H; Weis Bjerrum, Ole; Valent, Peter; Bindslev-Jensen, Carsten; Nilsson, Gunnar; Vestergaard, Hanne; Hägglund, Hans / Multidisciplinary Management of Mastocytosis : Nordic Expert Group Consensus. Acta Dermato-Venereologica, 2016. Bruun, Signe; Wedderkopp, Niels; Mølgaard, Christian; Kyhl, Henriette B; Zachariassen, Gitte; Hus- Dydensborg Sander, Stine; Størdal, Ketil; Plato Hansen, Tine; Nybo Andersen, Anne-Marie; Murray, by, Steffen / Using text messaging to obtain weekly data on infant feeding in a Danish birth cohort Joseph A; Lillevang, Søren Thue; Husby, Steffen / Validation of celiac disease diagnoses recorded resulted in high participation rates. Acta Paediatrica, Vol. 105, Nr. 6, 29.02.2016, s. 648–654. in the Danish National Patient Register using duodenal biopsies, celiac disease-specific antibodies, and human leukocyte-antigen genotypes. Clinical Epidemiology, Vol. 8, 2016, s. 789-799. Christesen, Henrik Boye Thybo; Pedersen, Birgitte Tønnes; Pournara, Effie; Petit, Isabelle Oliver; Júlíusson, Pétur Benedikt / Short Stature : Comparison of WHO and National Growth Standards/ Eg, Marianne; Cortes, Dina; Johansen, Anders; Frederiksen, Kirsten; Lorentzen, Vibeke; Larsen, References for Height. P L o S One, Vol. 11, Nr. 6, e0157277, 2016. Lone Marie; Holm, Jens- Christian; Vámosi, Marianne / Limited availability of childhood overweight and obesity treatment programmes in Danish paediatric departments. Danish Medical Journal, Vol. Christiansen, Elisabeth Soegaard; Kjaer, Henrik Fomsgaard; Eller, Esben; Bindslev-Jensen, Car- 63, Nr. 9, 09.2016, s. 1-5. sten; Høst, Arne; Mørtz, Charlotte G; Halken, Susanne / The prevalence of atopic diseases and the patterns of sensitization in adolescence. Pediatric allergy and immunology : official publication of the Fairchild, Timothy John; Klakk, Heidi ; Heidemann, Malene Søborg; Andersen, Lars Bo; Wedder- European Society of Pediatric Allergy and Immunology, 05.10.2016. kopp, Niels / Exploring the Relationship between Adiposity and Fitness in Young Children. Medicine and Science in Sports and Exercise, Vol. 48, Nr. 9, 09.2016, s. 1708-14. Christiansen, Elisabeth Soegaard; Andersen, Klaus Ejner; Bindslev-Jensen, Carsten; Halken, Su- sanne; Kjaer, Henrik Formsgaard; Eller, Esben; Høst, Arne; Mørtz, Charlotte G / Low patch test Frederiksen-Møller, Britta; Jørgensen, Jan S; Hansen, Mie R; Krigslund, Oliver; Vogel, Lotte K; An- reactivity to nickel in unselected adolescents tested repeatedly with nickel in infancy. Pediatric aller- dersen, Louise B; Jensen, Boye L / Prostasin and matriptase (ST14) in placenta from preeclamptic gy and immunology . 2016, vol. 27, nummer 6, 2016, s. 636-9. and healthy pregnant women. Journal of Hypertension, Vol. 34, Nr. 2, 02.2016, s. 298-306.

Cold, Frederik; Winther, Kristian Hillert; Christensen, Carina Ørts ; Iversen, Else Dalsgaard; Schøn- Frederiksen, Anja Lisbeth; Larsen, Martin Jakob; Brusgaard, Klaus; Norvack, Deborah V; Knudsen, nemann, Katrine Rahbek; Jeppesen, Stefan Starup; Illum, Niels Ove; Dahler-Eriksen, Bjarne Steen; Peter Juel Thiis; Schrøder, Henrik Daa; Qiu, Weimin; Eckhardt, Christina; McAlister, William H; Kas- Cold, Søren / Patientsamtale som eksamensfag. Ugeskrift for Laeger, Vol. 178/2, Nr. 2016-2, 178/2, sem, Moustapha; Mumm, Steven ; Nielsen, Morten Frost Munk; Whyte, Michael P / Neonatal High 2016, s. 186-187. Bone Mass With First Mutation Of The NF-κB Complex : Heterozygous De Novo Missense (p.Asp512Ser) RELA (Rela/p65). Journal of Bone and Mineral Research, Vol. 31, Nr. 1, 2016, s. 163 Dhami, Sangeeta; Nurmatov, Ulugbek; Roberts, Graham; Pfaar, Oliver; Muraro, Antonella; Ansote- –172. gui, Ignacio J; Calderon, Moises; Cingi, Cemal; Demoly, Pascal; Durham, Stephen; van Wijk, Ronald Gerth; Halken, Susanne; Hamelmann, Eckard; Hellings, Peter; Jacobsen, Lars; Knol, Edward; Lin- Garne, Kristina; Brødsgaard, Anne; Zachariassen, Gitte; Clemensen, Jane / Telemedicine in Neona- nemann, Desiree Larenas; Lin, Sandra; Maggina, Vivian; Oude- Elberink, Hanneke; Pajno, Giovan- tal Home Care : Identifying Parental Needs Through Participatory Design. J M I R Research Proto- ni; Panwankar, Ruby; Pastorello, Elideanna; Pitsios, Constantinos; Rotiroti, Giuseppina; Timmer- cols, Vol. 5, Nr. 3, 2016, s. e100. mans, Frans; Tsilochristou, Olympia; Varga, Eva-Maria; Wilkinson, Jamie; Williams, Andrew; Worm, Margitta; Zhang, Luo; Sheikh, Aziz / Allergen immunotherapy for allergic rhinoconjunctivitis : protocol Girma, Tsinuel; Kæstel, Pernille; Workeneh, Netsanet; Mølgaard, Christian; Eaton, Simon; Ander- for a systematic review. Clinical and Translational Allergy, Vol. 6, 12, 2016. sen, Gregers S; Michaelsen, Kim F; Friis, Henrik; Wells, Jonathan C K / Bioimpedance index for measurement of total body water in severely malnourished children : Assessing the effect of nutritio- Dalsager, Louise; Christensen, Nikolas; Husby, Steffen; Kyhl, Henriette; Nielsen, Flemming; Høst, nal oedema. Clinical Nutrition, Vol. 35, Nr. 3, 2016, s. 713-717. Arne; Grandjean, Philippe; Jensen, Tina Kold / Association between prenatal exposure to perfluorinated compounds and symptoms of infections at age 1-4 years among 359 children in the Odense Gjørup, Hans ; Kjaer, Inger; Beck-Nielsen, Signe; Poulsen, Mette R.; Haubek, Dorte / A Radiological Child Cohort. Environment International, Vol. 96, 05.09.2016, s. 58-64. study on intra- and extra-cranial calcifications in adults with X-linked hypophosphatemia and associations with other mineralizing enthesopathies and childhood medical treatment. Orthodontics & Cra- Dhami, Sangeeta; Nurmatov, Ulugbek; Halken, Susanne; Calderón, Moises A; Muraro, Antonella; niofacial Research, Vol. 19, Nr. 2, 01.05.2016, s. 114-125. Roberts, Graham; Du Toit, George; Kleine-Tebbe, Jörg; Larenas-Linnemann, Desiree; Lau, Su- sanne; Matricardi, Paolo Maria; Pajno, Giovanni; Papadopoulos, Nikolaos G; Pfaar, Oliver; Ryan, Hansen, Peter Martin; Pedersen, Rikke Neess; Lauridsen, Jørgen Trankjær; Toft, Palle / Intra Hospi- Dermot; Santos, Alexandra F; Timmermanns, Frans; Wahn, Ulrich; Sheikh, A / Allergen immuno- tal Delay Increases Mortality and Poor Outcome in Endovascular Coiling of Ruptured Intracranial therapy for the prevention of allergic disease : protocol for a systematic review. Pediatric Allergy and Aneurysms. International Journal of Clinical Anesthesiology, Vol. 4, Nr. 2, 1057, 2016. Immunology, Vol. 27, Nr. 3, 2016, s. 236-241. Hansen, Susanne; Hoffmann-Petersen, Benjamin; Sverrild, Asger; Bräuner, Elvira V; Lykkegaard, Jesper; Bodtger, Uffe; Agertoft, Lone; Korshøj, Lene; Backer, Vibeke / The Danish National Data- base for Asthma : establishing clinical quality indicators. European Clinical Respiratory Journal, Vol. 3, 2016, s. 33903. Hojsak, Iva; Colomb, Virginie; Braegger, Christian; Bronsky, Jiri; Campoy, Cristina; Domellöf, Mag- Jølving, Line Riis; Nielsen, Jan; Kesmodel, Ulrik Schiøler; Nielsen, Rasmus Gaardskjær; Beck- nus; Embleton, Nicholas; Fidler Mis, Nataša; Hulst, Jessie M; Indrio, Flavia; Lapillonne, Alexandre; Nielsen, Signe Sparre; Nørgård, Bente Mertz / Prevalence of maternal chronic diseases during Mihatsch, Walter; Molgaard, Christian; van Goudoever, Johannes; Fewtrell, Mary; ESPGHAN Com- pregnancy : a nationwide population based study from 1989 to 2013. Acta Obstetricia et Gynecologi- mittee on Nutrition / ESPGHAN Committee on Nutrition Position Paper. Intravenous Lipid Emulsions ca Scandinavica, Vol. 95, Nr. 11, 2016, s. 1295-1304. and Risk of Hepatotoxicity in Infants and Children : a Systematic Review and Meta-analysis. Journal of Pediatric Gastroenterology and Nutrition, Vol. 62, Nr. 5, 2016, s. 776-792. Kløvgaard, Marius; Nielsen, Nina Odgaard; Sørensen, Thomas Lund; Bjerregaard, Peter; Olsen, Britta; Christesen, Henrik Boye Thybo / Children in Greenland : disease patterns and contacts to the Holm, Kristina Garne / Telemedicine in Neonatal Home Care: Identifying parental needs through health care system. International Journal of Circumpolar Health, Vol. 75, 32903, 2016. participatory design. J M I R Research Protocols, Vol. 5, Nr. 3, 01.05.2016. Lange, Henriette Juel; Agertoft, Lone; Møller, Michael Boe; Clemmensen, Ole; Kielsgaard Kristen- Holst, G; Høst, A; Doekes, G; Meyer, H W; Madsen, A M; Plesner, K B; Sigsgaard, T / Allergy and sen, Thomas; Vestergaard, Hanne; Mørtz, Charlotte G; Bindslev-Jensen, Carsten; Broesby-Olsen, respiratory health effects of dampness and dampness-related agents in schools and homes : A Sigurd; Mastocytosis Centre Odense University Hospital (MastOUH) / A child with mastocytosis and cross-sectional study in Danish pupils. Indoor Air, Vol. 26, 2016, s. 880-891. lymphomatoid papulosis. Clinical Case Reports, Vol. 4, Nr. 5, 2016, s. 517-519.

Horsten, Hans-Henrik; Fisker, Niels; Bygum, Anette / Eczema Coxsackium Caused by Coxsackievi- Larnkjær, Anni; Bruun, Signe; Pedersen, Dorthe; Zachariassen, Gitte; Barkholt, Vibeke; Agostoni, rus A6. Pediatric Dermatology, 18.04.2016. Carlo; Mølgaard, Christian; Husby, Steffen; Michaelsen, Kim F / Free Amino Acids in Human Milk and Associations with Maternal Anthropometry and Infant Growth. Journal of Pediatric Gastroentero- Illum, Niels Ove; Bonderup, Mette; Gradel, Kim Oren / Environmental needs in childhood disability logy and Nutrition, Vol. 63, Nr. 3, 2016, s. 374-378. analysed by the WHO ICF, Child and Youth Version. Danish Medical Journal, Vol. 63, Nr. 6, A5238, 06.2016. Larsen, Kristian Traberg; Huang, Tao; Ried-Larsen, Mathias; Andersen, Lars Bo; Heidemann, Male- ne; Møller, Niels Christian / A Multi-Component Day-Camp Weight-Loss Program Is Effective in Jelsig, Anne Marie; Ousager, Lilian Bomme; Brusgaard, Klaus; Qvist, Niels / Juvenile Polyps in Den- Reducing BMI in Children after One Year : A Randomized Controlled Trial. P L o S One, Vol. 11, Nr. mark From 1995 to 2014. Diseases of the Colon and Rectum, Vol. 59, Nr. 8, 08.2016, s. 751-757. 6, e0157182, 2016.

Jelsig, Anne Marie; Brusgaard, Klaus; Hansen, Tine Plato; Qvist, Niels; Larsen, Martin; Bojesen, Lassen, Tina Harmer; Frederiksen, Hanne; Kyhl, Henriette Boye; Swan, Shanna H; Main, Katharina Anders; Nielsen, Claus Buhl; Ousager, Lilian Bomme / Germline variants in Hamartomatous Polypo- M; Andersson, Anna- Maria; Lind, Dorte Vesterholm; Husby, Steffen; Wohlfahrt-Veje, Christine; sis Syndrome-associated genes from patients with one or few hamartomatous polyps. Scandinavian Skakkebæk, Niels E; Jensen, Tina Kold / Prenatal Triclosan Exposure and Anthropometric Measu- Journal of Gastroenterology, Vol. 51, Nr. 9, 2016, s. 1118-1125. res Including Anogenital Distance in Danish Infants. Environmental Health Perspectives, Vol. 124, Nr. 8, 08.2016, s. 1261-8. Jelsig, A M; Tørring, P M; Kjeldsen, A D; Qvist, N; Bojesen, A; Jensen, U B; Andersen, M K; Gerdes, A M; Brusgaard, K; Ousager, L B / JP-HHT phenotype in Danish patients with SMAD4 mutations. Lind DV, Priskorn L, Lassen TH, Nielsen F, Kyhl HB, Kristensen DM, Christesen HT, Steener J, Clinical Genetics, Vol. 90, Nr. 1, 2016, s. 55-62. Grandjean P, Jensen TK. / Prenatal exposure to perfluoroalkyl substances and anogenital distance at 3 months of age as marker of endocrine disruption. Reprod Toxicol. 2016 Jul 12. pii: S0890-6238 Jelsig, A M; Qvist, N; Sunde, L; Brusgaard, K; Hansen, T; Wikman, F P; Nielsen, C.B.; Nielsen, I K; (16)30265-9. Gerdes, A M; Bojesen, A; Ousager, L B / Disease pattern in Danish patients with Peutz-Jeghers syndrome. International Journal of Colorectal Disease, Vol. 31, Nr. 5, 2016, s. 997-1004. Lings, Kristina; Lauridsen, Mathilde Faurholdt; Hansen, Lars Kjærsgaard / Sjældent syndrom som differentialdiagnose ved markant fregnedannelse. Ugeskrift for laeger, Vol. 178, Nr. 47, 21.11.2016. Jensen, Tina Kold; Andersen, Louise Bjørkholt; Kyhl, Henriette Boye; Nielsen, Flemming; Christe- sen, Henrik Thybo; Grandjean, Philippe / Correction : Association between Perfluorinated Com- Ludvigsson, Jonas F; Agreus, Lars; Ciacci, Carolina; Crowe, Sheila E; Geller, Marilyn G; Green, pound Exposure and Miscarriage in Danish Pregnant Women. P L o S One, Vol. 11, Nr. 2, Peter H R; Green, Peter H R; Hungin, A Pali; Koletzko, Sibylle; Koltai, Tunde; Lundin, Knut E A; e0149366, 10.02.2016, s. 1-2. Mearin, M Luisa; Murray, Joseph A; Reilly, Norelle; Walker, Marjorie M; Sanders, David S; Shamir, Raanan; Troncone, Riccardo; Husby, Steffen / Transition from childhood to adulthood in coeliac Jensen, Tina K.; Frederiksen, Hanne; Kyhl, Henriette B.; Lassen, Tina Harmer; Swan, Shanna H.; disease : the Prague consensus report. Gut, Vol. 65, Nr. 8, 2016, s. 1242-51. Bornehag, Carl-Gustaf; Skakkebaek, Niels E.; Main, Katharina M.; Lind, Dorte V; Husby, Steffen; Andersson, Anna-Maria / Prenatal Exposure to Phthalates and Anogenital Distance in Male Infants from a Low-Exposed Danish Cohort (2010-2012). Environmental Health Perspectives, Vol. 124, Nr. 7, 2016, s. 1107–1113. Luef, Birgitte M; Andersen, Louise B; Renault, Kristina Martha ; Nohr, Ellen A; Jørgensen, Jan S; Risby, Kirsten; Jakobsen, Marianne Skytte; Qvist, Niels / Congenital Abdominal Wall Defects : Sta- Christesen, Henrik Boye Thybo / Validation of hospital discharge diagnoses for hypertensive disor- ged closure by Dual Mesh. Journal of neonatal surgery, Vol. 5, Nr. 1, 2, 23.01.2016. ders of pregnancy. Acta Obstetricia et Gynecologica Scandinavica, Vol. 95, Nr. 11, 2016, s. 1288- 1294. Schlosser, Anders; Pilecki, Bartosz; Hemstra, Line E; Kejling, Karin; Kristmannsdottir, Gudlaug B; Wulf-Johansson, Helle; Moeller, Jesper B; Füchtbauer, Ernst-Martin; Nielsen, Ole; Kirketerp-Møller, Lykkedegn S, Beck-Nielsen SS, Sorensen GL, Andersen LB, Fruekilde PB, Nielsen J, Kyhl HB, Katrine; Dubey, Lalit K; Hansen, Pernille B L; Stubbe, Jane; Wrede, Christoph; Hegermann, Jan; Joergensen JS, Husby S, Christesen HT. / Vitamin D supplementation, cord 25-hydroxyvitamin D Ochs, Matthias; Rathkolb, Birgit; Schrewe, Anja; Bekeredjian, Raffi; Wolf, Eckhard; Gailus-Durner, and birth weight: Findings from the Odense Child Cohort. Clin Nutr. 2016 Oct 27. pii: S0261-5614 Valérie; Fuchs, Helmut; de Angelis, Martin Hrabě; Lindholt, Jes S; Holmskov, Uffe; Sorensen, Grith (16)31283-3 L / MFAP4 Promotes Vascular Smooth Muscle Migration, Proliferation and Accelerates Neointima Formation. Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 36, 2016, s. 122-133. Lykkedegn, Sine; Sorensen, Grith Lykke; Beck-Nielsen, Signe Sparre; Pilecki, Bartosz; Duelund, Lars; Marcussen, Niels; Christesen, Henrik Boye Thybo / Vitamin D Depletion in Pregnancy Decrea- Schrøder, Henrik; Rechnitzer, Catherine; Wehner, Peder Skov; Rosthøj, Steen; Møller, Jens Kjøl- ses Survival Time, Oxygen Saturation, Lung Weight and Body Weight in Preterm Rat Offspring. P L seth; Lausen, Birgitte; Petersen, Gitte; Nørgaard, Mette / Danish Childhood Cancer Registry. Clinical o S One, Vol. 11, Nr. 8, 2016, s. e0155203. Epidemiology, Vol. 8, 2016, s. 461-464.

Milićević, Novica M; Schmidt, Friederike; Kunz, Natalia; Kalies, Kathrin; Milićević, Živana; Schlosser, Shen, René Liang; Pontoppidan, Peter Erik Lotko; Rathe, Mathias; Jiang, Pingping; Hansen, Carl Anders; Holmskov, Uffe; Sorensen, Grith Lykke; Westermann, Jürgen / The role of microfibrillar- Frederik; Buddington, Randal K; Heegaard, Peter M H; Müller, Klaus; Sangild, Per T / Milk Diets associated protein 4 (MFAP4) in the formation and function of splenic compartments during embryo- Influence Doxorubicin-Induced Intestinal Toxicity in Piglets. American Journal of Physiology: Gastro- nic and adult life. Cell and Tissue Research, Vol. 365, Nr. 1, 22.02.2016, s. 135-145. intestinal and Liver Physiology, 21.07.2016, s. ajpgi.00373.2015

Mossin, Mats H; Aaby, Jens B; Dalgård, Christine; Lykkedegn, Sine; Christesen, Henrik T; Bilen- Shen, René Liang; Rathe, Mathias; Jiang, Pingping; Pontoppidan, Peter Erik Lotko; Heegaard, Peter berg, Niels / Inverse associations between cord vitamin D and attention deficit hyperactivity disorder M H; Müller, Klaus; Sangild, Per Torp / Doxorubicin-Induced Gut Toxicity in Piglets fed Bovine Milk symptoms : A child cohort study. Australian & New Zealand Journal of Psychiatry, 30.09.2016. and Colostrum. Journal of Pediatric Gastroenterology and Nutrition.

Mölleken, Christian; Poschmann, Gereon; Bonella, Francesco; Costabel, Ulrich; Sitek, Barbara; Sildorf, S M; Hertel, N T; Thomsen, J; Fredheim, S; Hastrup, H; Pipper, C; Hertz, B; Svensson, J / Stühler, Kai; Meyer, Helmut E; Schmiegel, Wolff H; Marcussen, Niels; Helmer, Carl Michael; Niel- Treatment intensification without improved HbA1c levels in children and adolescents with Type 1 sen, Ole; Hansen, Søren Werner Karlskov; Schlosser, Anders; Holmskov, Uffe; Sorensen, Grith diabetes mellitus. Diabetic Medicine, Vol. 33, Nr. 4, 2016, s. 515-522. Lykke / MFAP4 : a candidate biomarker for hepatic and pulmonary fibrosis? Sarcoidosis Vasculitis and Diffuse Lung Diseases, Vol. 33, Nr. 1, 2016, s. 41-50. Svensson, Jannet; Cerqueira, Charlotte; Kjærsgaard, Per; Lyngsøe, Lene; Hertel, Niels Thomas; Madsen, Mette; Mortensen, Henrik B; Johannesen, Jesper / Danish Registry of Childhood and Ado- Petersen, Thomas Houmann; Agertoft, Lone / Corticosteroids for Allergic Rhinitis. Current Treatment lescent Diabetes. Clinical Epidemiology, Vol. 8, 2016, s. 679-683. Options in Allergy, Vol. 3, Nr. 1, 2016, s. 18-30. Szajewska, Hania; Shamir, Raanan; Mearin, Luisa; Ribes-Koninckx, Carmen; Catassi, Carlo; Do- Pilecki, Bartosz; Holm, Anne T; Schlosser, Anders; Moeller, Jesper B; Wohl, Alexander P; Zuk, Ale- mellöf, Magnus; Fewtrell, Mary S; Husby, Steffen; Papadopoulou, Alexandra; Vandenplas, Yvan; xandra V; Heumüller, Stefanie E; Wallis, Russell; Moestrup, Soren K; Sengle, Gerhard; Holmskov, Castillejo, Gemma; Kolacek, Sanja; Koletzko, Sibylle; Korponay-Szabó, Ilma R; Lionetti, Elena; Po- Uffe; Sorensen, Grith L / Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropo- lanco, Isabel; Troncone, Riccardo / Gluten Introduction and the Risk of Coeliac Disease : A Position elastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation. Journal of Biological Chemi- Paper by the European Society for Pediatric Gastroenterology, Hepatology, and Nutrition. Journal of stry, Vol. 291, Nr. 3, 15.01.2016, s. 1103-14. Pediatric Gastroenterology and Nutrition, Vol. 62, Nr. 3, 03.2016, s. 507-13.

Rathe, Mathias; Thomassen, Mads; Shen, René L.; Pontoppidan, Peter E L; Husby, Steffen; Müller, Tinggaard, Jeanette ; Wohlfahrt-Veje, C.; Husby, S; Christiansen, L; Skakkebaek, N. E.; Jensen, T Klaus; Kruse, Torben A.; Sangild, Per T. / Chemotherapy Modulates Intestinal Immune Gene Ex- K; Grandjean, P; Main, K. M.; Andersen, H R / Prenatal pesticide exposure and PON1 genotype pression Including Surfactant Protein-D and Deleted in Malignant Brain Tumors 1 in Piglets. Chemo- associated with adolescent body fat distribution evaluated by dual X-ray absorptiometry (DXA). therapy, Vol. 61, Nr. 4, 18.02.2016, s. 204-216. Andrology, Vol. 4, Nr. 4, 2016, s. 735–744.

Ravn, Kirstine; Neland, Mette; Wibrand, Flemming; Duno, Morten; Ostergaard, Elsebet / Hearing impairment and renal failure associated with RMND1 mutations. American Journal of Medical Gene- tics. Part A, Vol. 170, Nr. 1, 2016, s. 142–147. Twigg, Stephen R F; Ousager, Lilian Bomme; Miller, Kerry A; Zhou, Yan; Elalaoui, Siham C; Sefiani, Abdelaziz; Bak, G. S; Hove, Hanne; Kjærsgaard Hansen, Lars; Fagerberg, Christina R; Tajir, Mari- am; Wilkie, Andrew O M / Acromelic frontonasal dysostosis and ZSWIM6 mutation : phenotypic spectrum and mosaicism. Clinical Genetics, Vol. 90, Nr. 3, 2016, s. 270-275.

Yilma, Daniel; Kæstel, Pernille; Olsen, Mette F; Abdissa, Alemseged; Tesfaye, Markos; Girma, Tsi- nuel; Krarup, Henrik; Mølgaard, Christian; Michaelsen, Kim F; Ritz, Christian; Kirk, Ole; Andersen, Åse B; Friis, Henrik / Change in serum 25- hydroxyvitamin D with antiretroviral treatment initiation and nutritional intervention in HIV-positive adults. The British Journal of Nutrition, Vol. 116, Nr. 10, 2016, s. 1720-1727.

Watson, Alastair; Kronqvist, Nina; Spalluto, C Mirella; Griffiths, Mark; Staples, Karl J; Wilkinson, Tom; Holmskov, Uffe; Sorensen, Grith L; Rising, Anna; Johansson, Jan; Madsen, Jens; Clark, Ho- ward / Novel expression of a functional trimeric fragment of human SP-A with efficacy in neutralisati- on of RSV. Immunobiology, Vol. 222, Nr. 2, 02.2017, s. 111-118.

Zachariassen, G; Hyldig, N; Joergensen, J S; Nielsen, D S; Greisen, G / The half-life and exposure of Cefuroxime varied in newborn infants after a Caesarean section. Acta Paediatrica, Vol. 105, Nr. 9, 2016, s. 1074-1078. HCA Research is responsible for teaching medical students in paediatrics and students at other related educations.

Teachers 12 ph.d. students Education: Medicine bachelor

9 associate professors Reproduction and pharmacodynamics 3 professors

Education: Medicine candidate

Respiratory Other educations

Musculoskeletal Medical students

in the clinic Blood and blood-forming organs

Pharmacy Nutrition and metabolism students TEACHING

Mental and behavioral disturbances Ad. hoc

teaching Mother and child

Clinical courses First of January 2016 Joseph A. Murray from The Mayo Clinic in Minnesota, USA, became Adjunct Professor at Hans Christian Andersen Children’s Hospital and Department of Clinical Research, University of Southern Denmark.

Dr. Murray studied medicine at National University of Ireland and internal medicine/gastroenterology training at Trinity College and Beaumont Hospital (Dublin) and the University of Iowa. He was on the faculty of the University of Iowa (1990 -1998) before joining the Division of Gastroenterology at Mayo Clinic in Roche- ster, MN in 1998, becoming Professor of Medicine in 2004. Dr. Murray has a strong background/experience in basic/clinical gastroenterology, and his work spans basic/translational research in celiac disease focusing on immunology, genetics of the disease and immune responses to food and microbiota. He is the author/co-author of more than 235 scientific publications and 33 chapters, and his work is funded by the NIH, private foundations and commercial entities. He is past president of North American Society for the Study of Celiac Disease, serves on advisory boards for lay support groups, and consults with several companies on the topic of celiac disease.

The Inaugural Seminar took place at June 16. In addition Joseph A. Murray ca- me to visit November 22 and a seminar about the fate of undiagnosed celiac NEW ADJUNCT disease was held. PROFESSOR

Each Spring HCA Research celebrates the birthday of Hans Christian Andersen by inviting employees at the children’s hospital and collabora- tors to a research seminar about new projects and results. Junior and senior researchers present new projects and results. In 2016 around 85 people attended the seminar.

RESEARCH SEMINAR

In 2016 HCA Research received around 1,4 million DKK in cont- ract and grant awards from private and public foundations.

FUND GRANT

OUH prægraduatpulje 60.000

OUH og RH forskningspulje 252.865

OUH Internaonaliseringspuljen 224.950

Børnecancerfonden 308.000

Aase og Ejnar Danielsens Fond 150.000

Fonden for Lægevidenskabens Fremme 150.000 GRANTS Ocon 253.375 TOTAL 1.399.190 DKK

Odense University Hospital Hans Christian Andersen Children’s Hospital HCA Forskning Kløvervænget 23C DK-5000 Odense C

Professor and head of research Steffen Husby [email protected] CONTACT +45 2034 2837