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(12) United States Patent (10) Patent No.: US 9,493.826 B2 Ismagilov Et Al

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(12) United States Patent (10) Patent No.: US 9,493.826 B2 Ismagilov Et Al USOO9493.826B2 (12) United States Patent (10) Patent No.: US 9,493.826 B2 Ismagilov et al. (45) Date of Patent: Nov. 15, 2016 (54) MULTIVOLUME DEVICES, KITS AND (52) U.S. Cl. RELATED METHODS FOR CPC ....... CI2O I/6851 (2013.01); B0IL 3/502738 QUANTIFICATION AND DETECTION OF (2013.01); B01 F 13/0094 (2013.01); B0IL NUCLEC ACDS AND OTHER ANALYTES 3/5025 (2013.01); B0IL 3/50851 (2013.01); B01 L 2200/027 (2013.01); (71) Applicants: California Institute of Technology, (Continued) Pasadena, CA (US); University of Chicago, Chicago, IL (US) (58) Field of Classification Search CPC ................... B01L1100/027; B01L 3/502738; (72) Inventors: Rustem F. Ismagilov, Altadena, CA B01L 2300/00864; B01L 3/5052; B01F (US); Feng Shen, Pasadena, CA (US); 13/00; B01F 13/0094 Jason E. Kreutz, Marysville, WA (US); See application file for complete search history. Wenbin Du, Wenzhou (CN); Bing Sun, Pasadena, CA (US) (56) References Cited (73) Assignees: CALIFORNLA INSTITUTE OF U.S. PATENT DOCUMENTS TECHNOLOGY, Pasadena, CA (US); 2,541,413 A 2/1951 Gorey UNIVERSITY OF CHICAGO, 3,787,290 A 1/1974 Kaye Chicago, IL (US) (Continued) (*) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 FOREIGN PATENT DOCUMENTS U.S.C. 154(b) by 59 days. CN 2482O70 Y 3, 2002 CN 1886644 A 12/2006 (21) Appl. No.: 14/177,190 (Continued) (22) Filed: Feb. 10, 2014 OTHER PUBLICATIONS (65) Prior Publication Data US 7,897.368, 03/2011, Handique et al. (withdrawn) US 2014/03.36064 A1 Nov. 13, 2014 (Continued) Related U.S. Application Data Primary Examiner—Jonathan Hurst (63) Continuation of application No. 13/467,482, filed on (74) Attorney, Agent, or Firm — Fenwick & West LLP May 9, 2012, which is a continuation-in-part of application No. 13/440,371, filed on Apr. 5, 2012, (57) ABSTRACT now Pat. No. 9,447.461, which is a Provided are devices comprising multivolume analysis continuation-in-part of regions, the devices being capable of Supporting amplifica (Continued) tion, detection, and other processes. Also provided are related methods of detecting or estimating the presence (51) Int. Cl. nucleic acids, viral levels, and other biological markers of CI2O I/68 (2006.01) interest. BOIF 1.3/OO (2006.01) BOIL 3/OO (2006.01) 14 Claims, 17 Drawing Sheets US 9,493.826 B2 Page 2 Related U.S. Application Data 6,503,707 1, 2003 Baxter-Lowe ... 435/6.12 application No. 13/257,811, filed as application No. 6,524.456 B1 2, 2003 Ramsey et al. PCT/US2010/028316 on Mar. 23, 2010, now Pat. No. 6,548.256 B2 4/2003 Lienau et al. 6,550,497 B2 4/2003 Thiele et al. 9,415,392. 6,565,813 B1 5/2003 Garyantes 6,567,492 B2 5/2003 Kiselev et al. (60) Provisional application No. 61/262,375, filed on Nov. 6,569,631 B1 5/2003 Pantoliano et al. 18, 2009, provisional application No. 61/162.922, 6,575,188 B2 6, 2003 Parunak filed on Mar. 24, 2009, provisional application No. 6,606,618 B2 8, 2003 Delo 61/340,872, filed on Mar. 22, 2010, provisional ap 6,632,653 B1 10, 2003 Astle 6,638.408 B1 10, 2003 Speicher et al. plication No. 61/518,601, filed on May 9, 2011, 6,702.256 B2 3, 2004 Killeen et al. provisional application No. 61/516,628, filed on Apr. 6,705.357 B2 3, 2004 Jeon et al. 5, 2011. 6,716,642 B1 4, 2004 Wu et al. 6,717, 136 B2 4, 2004 Andersson et al. (52) U.S. Cl. 6,720,187 B2 4, 2004 Bedingham et al. CPC .................. B01 L 2200/0605 (2013.01); B0IL 6,737,026 B1 5, 2004 Bergh et al. 2200/0642 (2013.01); B0IL 2300/045 6,797,056 B2 9, 2004 David (2013.01); BOIL 2300/049 (2013.01); BOIL 6,808,934 B2 10, 2004 Mutz et al. 6,821,770 B1 11/2004 Hogan 2300/0803 (2013.01); B01L 2300/0816 6,845,968 B2 1/2005 Killeen et al. (2013.01); B01 L 2300/0864 (2013.01); BOIL 6,852,851 B1 2, 2005 Tooke et al. 2400/0406 (2013.01); B0IL 2400/065 6,855.490 B2 2, 2005 Sompuram et al. (2013.01); B01 L 2400/0644 (2013.01) 6,858.439 B1 2, 2005 Xu et al. 6,883.559 B2 4, 2005 Jeon et al. (56) References Cited 6,893,612 B2 5/2005 Kacian et al. 6,949,355 B2 9, 2005 Yamanishi et al. 6,949,575 B2 9, 2005 Barta et al. U.S. PATENT DOCUMENTS 6,994,749 B2 2, 2006 David 7,003,104 B2 2, 2006 Lee 3.996,345 A 12/1976 Ullman et al. 7,015,041 B2 3, 2006 Santarsiero et al. 4,071.409 A 1/1978 Messing et al. 7,101,663 B2 9, 2006 Godfrey et al. 4,755,363 A 7/1988 Fujita et al. 7,122,301 B2 10, 2006 Shvets et al. 4,853,336 A 8, 1989 Saros et al. 7,122,640 B2 10, 2006 Gjerde et al. 4,963,498 A 10, 1990 Hillman et al. 7,126,626 B2 10, 2006 Sawahara et al. 5,026,113 A 6, 1991 DiCarlo et al. 7,129,091 B2 10, 2006 Ismagilov et al. 5,114,208 A 5, 1992 Ikeda et al. 7,135,180 B2 11/2006 Truong-Le 5,169,942 A 12/1992 Johnson et al. 7,136,688 B2 11/2006 Jung et al. 5,185,099 A 2/1993 Delpuech et al. 7,169,601 B1 1/2007 Northrup et al. 5,251,670 A 10, 1993 Bates et al. 7,235,216 B2 6, 2007 Kiselev et al. 5,264,570 A 11/1993 Johnson et al. 7,244,961 B2 7/2007 Jovanovich et al. 5,478,893 A 12/1995 Ghosh et al. 7,252,939 B2 8, 2007 Mori et al. 5,656,493 A 8, 1997 Mullis et al. 7,294,308 B2 11/2007 Kacian et al. 5,686,315 A 11/1997 Pronovost et al. 7,294,466 B2 11/2007 McMillan 5,688,651 A 11/1997 Solomon 7,294,503 B2 11/2007 Quake et al. 5,707,850 A 1, 1998 Cole 7,297,485 B2 11/2007 Bornarth et al. 5,725,017 A 3/1998 Elsberry et al. 7,306,672 B2 12, 2007 Hansen et al. 5,726,026 A 3/1998 Wilding et al. 7,309,588 B2 12, 2007 Burg et al. 5,739,036 A 4, 1998 Parris 7,314,070 B2 1, 2008 Jeon et al. 5,744,305 A 4, 1998 Fodor et al. 7,319,003 B2 1, 2008 Cantor et al. 5,746,978 A 5, 1998 Bienhaus et al. 7,329,485 B2 2, 2008 Zlotnick 5,772,889 A 6/1998 Gjerde et al. 7,351,303 B2 4/2008 Liu et al. 5,773.258 A 6, 1998 Birch et al. 7,375, 190 B2 5/2008 Cheng et al. 5,805.947 A 9/1998 Miyamoto et al. 7,413,712 B2 8, 2008 Liu et al. 5,872,010 A 2/1999 Karger et al. 7.465,562 B2 12, 2008 Wangh et al. 5.948,624 A 9, 1999 Rothschild et al. 7,501,245 B2 3, 2009 Quake et al. 5,993,631 A 11/1999 Parton et al. 7,556,776 B2 T/2009 Fraden et al. 5.997,636 A 12/1999 Gamarnik et al. 7,595,871 B2 9, 2009 Weber 6,013,166 A 1/2000 Heller 7,608,399 B2 10, 2009 Reed et al. 6,130,098 A 10/2000 Handique et al. 7,615,274 B2 11/2009 Ehrfeld et al. 6,140,053 A 10, 2000 Koster 7,629,165 B2 12, 2009 Wyatt et al. 6,146,854 A 11/2000 Koster et al. 7,648,835 B2 1, 2010 Breidford et al. 6,168,948 B1 1/2001 Anderson et al. 7,655,129 B2 2, 2010 Blackburn et al. 6,171,785 B1 1/2001 Higuchi 7,683,035 B1 3, 2010 Erbacher et al. 6,180.372 B1 1/2001 Franzen 7,767.447 B2 8, 2010 Breidenthal et al. 6, 197,595 B1 3/2001 Anderson et al. 7,780,336 B2 8, 2010 Breidenthal et al. 6,203,989 B1 3/2001 Goldberg et al. 7,790,865 B1 9, 2010 Heath et al. 6,274,726 B1 8/2001 Laugharn, Jr. et al. 7,846,333 B2 12/2010 Pluester et al. 6,277,648 B1 8/2001 Colpan B1 12/2010 Sagripanti 6,300,138 B1 10/2001 Gleason et al. 7,851,207 6,379,929 B1 4/2002 Burns et al. 7,867,757 B2 1, 2011 Karlsen et al. 6,391,624 B1* 5/2002 Megerle ............... C12O 1/6825 7,871,813 B2 1, 2011 Wyatt et al. 422.68.1 7,915.030 B2 3, 2011 Inoue et al. 6,409,832 B2 6/2002 Weigl et al. 7,939,018 B2 5, 2011 Bedingham et al. 6.426.230 B1 7/2002 Feistel 7,939,249 B2 5, 2011 Parthasarathy et al. 6,436,292 B1 8, 2002 Petro 7,955,504 B1 6, 2011 Jovanovic et al. 6,451,610 B1 9/2002 Gorman et al. 7.998,708 B2 8, 2011 Handique et al.
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