Yeast Metabolic and Signaling Genes Are Required for Heat-Shock Survival
Yeast metabolic and signaling genes are required for PNAS PLUS heat-shock survival and have little overlap with the heat-induced genes Patrick A. Gibneya,b,1, Charles Lub,1, Amy A. Caudya,2,3, David C. Hessc,3, and David Botsteina,b,4 aLewis-Sigler Institute for Integrative Genomics and bDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544; and cDepartment of Biology, Santa Clara University, Santa Clara, CA 95053 Contributed by David Botstein, September 30, 2013 (sent for review August 25, 2013) Genome-wide gene-expression studies have shown that hundreds or proteins that increase in abundance as part of the heat-shock of yeast genes are induced or repressed transiently by changes in response (1, 11–14). Classically, this set of genes/proteins includes temperature; many are annotated to stress response on this basis. the molecular chaperones, which often are induced in response to To obtain a genome-scale assessment of which genes are function- stress (14). However, by using the deletion collection to assay ally important for innate and/or acquired thermotolerance, we thermotolerance, we also can identify genes important for stress combined the use of a barcoded pool of ∼4,800 nonessential, pro- resistance that are either constitutively present or posttransla- totrophic Saccharomyces cerevisiae deletion strains with Illumina- tionally modified. We therefore carried out two kinds of exper- based deep-sequencing technology. As reported in other recent iment to examine heat-stress resistance. First we studied the 50 °C studies that have used deletion mutants to study stress responses, survival time-course of samples from a steady-state chemostat cul- we observed that gene deletions resulting in the highest thermo- ture of the entire prototrophic barcoded deletion collection.
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