Epidemiology of Foot and Mouth Disease in Buffaloes and Cattle Of

Total Page:16

File Type:pdf, Size:1020Kb

Epidemiology of Foot and Mouth Disease in Buffaloes and Cattle Of Epidemiology of Foot and Mouth Disease in Buffaloes and Cattle of Punjab By Muhammad Zeeshan Nawaz M.Sc. (Hons.) A thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy In Microbiology INSTITUTE OF MICROBIOLOGY FACULTY OF VETERINARY SCIENCE UNIVERSITY OF AGRICULTURE FAISALABAD, PAKISTAN 2014 0 To My Affectionate Parents I would like to dedicate this manuscript to my Honorable & Respected father Sheikh Muhammad Nawaz, who supported and encouraged me a lot at every moment during my degree program and made possible for me to complete my Doctorate. Special dedication to my mother also who never fail to remember me for a second in her prayers for my progress and success 1 CONTENTS CHAPTER TITLE PAGE ACKNOWLEDGEMENT i LIST OF TABLES ii LIST OF FIGURES iii 1 INTRODUCTION 01 2 REVIEW OF LITERATURE 04 3 MATERIALS AND METHODS 21 4 RESULTS 46 5 DISCUSSION 86 6 SUMMARY 93 7 LITERATURE CITED 95 8 ANNEXTURES 112 2 ACKNOWLEDGEMENTS If oceans turn into ink and all the woods become pens, even then the praise of Allah Almighty cannot be expressed. He, who created the universe and knows whatever, is there in it, hidden or evident and who bestowed upon me the intellectual ability and wisdom to search for its secrets. Countless salutations be upon the Holy Prophet (Salutations and peace of Allah be upon him), the city of knowledge who guided his ‘Ummah” to seek knowledge from cradle to grave. I feel highly privilege to ascribe the most and ever burning flame of gratitude and deep sense of devotion to my reverend supervisor Dr. Muhammad Arshad, Professor Institute of Microbiology, University of Agriculture, Faisalabad, for his keen potential interest, sagacious suggestions, consistent encouragement, constructive criticism, incentive teachings, polite behavior, guardian and sympathetic attitude, step to step guidance and dynamic supervision during the conduct of these investigations and in preparation of this manuscript. It gives me great pleasure in expressing my gratitude with the profound sense of benediction to Prof. Dr. Sajjad ur Rahman, Institute of Microbiology, University of Agriculture, Faisalabad, for his cooperation, scholastic guidance, immense moral support, keen interest, unerring vigor, staunch help, masterly advice and constructive suggestions. I offer my special and sincere thanks to a dignified personality Prof. Dr. Zafar Iqbal, Department of Parasitology, University of Agriculture, Faisalabad, and Prof. Dr. Iftikhar Hussain, Director, Institute of Microbiology, University of Agriculture, Faisalabad, for his valuable suggestions and guidance. Family is truing dense of my humblest obligations and my words will fail to give them credit especially to my Parents, Sisters and Wife whose prayers, love, patience, sacrifice, encouragement and spiritual inspiration was a great source of motivation for the quest of knowledge at every stage of my education. I would not be doing justice without acknowledging the invaluable moral support, excellent cooperation and constant encouragement extended to me by Saif ur Rehman, Bahar e Mustafa, Shahid Ali, Saad Zubair, Umair Hassan, Iftikhar bhai and Intezar Hussain. I fervently extended my zealous, sincerest and warmest thanks to my friends for which I could not mention, for their endless and excellent cooperation and moral support. Finally, I again thank all those whom I have mentioned and whom I could not, for helping me in completing this work, as it is customary, the errors that remain are entirely mine. At the end I gratefully acknowledge the financial support provided by Higher Education Commission (HEC) Pakistan, Indigenous Scholarship Phase VI for the completion of this work along with the Endowment Fund Secretariat (EFS), University of Agriculture, Faisalabad, Pakistan for their support and logistics. Muhammad Zeeshan Nawaz 3 LIST OF TABLES Page No. Title No. Climatic and geographic detail of three districts of Punjab selected for the 3.1 24 seroprevalence of Foot and Mouth Disease Area & species related distribution of samples for seroprevalence of Foot 3.2 28 and Mouth Disease Sex related distribution of samples for seroprevalence of Foot and Mouth 3.3 29 Disease Age related distribution of samples for seroprevalence of Foot and Mouth 3.4 30 Disease Pregnancy status related distribution of samples for seroprevalence of Foot 3.5 31 and Mouth Disease Herd type related distribution of samples for seroprevalence of Foot and 3.6 32 Mouth Disease Distribution of samples for molecular detection of Foot and Mouth Disease 3.7 35 virus Sequences for universal and serotype specific primers of Foot and Mouth 3.8 39 Disease Different experimental groups for Foot and Mouth Disease vaccines 3.9 43 evaluation Comparative seroprevalence of Foot and Mouth Disease in three districts 4.1 47 of Punjab Comparative sex related seroprevalence of Foot and Mouth Disease in 4.2 49 three districts of Punjab 4 Page No. Title No. Comparative age related seroprevalence of Foot and Mouth Disease in 4.3 50 three districts of Punjab Comparative pregnancy related seroprevalence of Foot and Mouth Disease 4.4 51 in three districts of Punjab Comparative herd type related seroprevalence of Foot and Mouth Disease 4.5 53 in three districts of Punjab Molecular detection of Foot and Mouth Disease virus by Reverse 4.6 57 Transcriptase –Polymerase Chain Reaction Serotyping of Foot and Mouth Disease virus by Indirect Sandwich-Enzyme 4.7 62 Linked Immunosorbent Assay Serotyping of Foot and Mouth Disease virus by Reverse Transcriptase – 4.8 63 Polymerase Chain Reaction Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.9 68 virus, Serotype ‘O’ in buffaloes and cattle at 2 weeks Post Vaccination Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.10 69 virus, Serotype ‘O’ in buffaloes and cattle at 4 weeks Post Vaccination Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.11 70 virus, Serotype ‘O’ in buffaloes and cattle at 6 weeks Post Vaccination Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.12 71 virus, Serotype ‘O’ in buffaloes and cattle at 8 weeks Post Vaccination Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.13 72 virus, Serotype ‘O’ in buffaloes and cattle at 10 weeks Post Vaccination Comparative Geometric Mean Titers and Cumulative Mean Titers of 4.14 73 different vaccines against serotype ‘O’ of Foot and Mouth Disease virus 5 Page No. Title No. Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.15 74 virus, Serotype ‘A’ in buffaloes and cattle at 2 weeks Post Vaccination Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.16 75 virus, Serotype ‘A’ in buffaloes and cattle at 4 weeks Post Vaccination 4.17 Indirect Hemagglutination antibody titers against Foot and Mouth Disease 76 virus, Serotype ‘A’ in buffaloes and cattle at 6 weeks Post Vaccination Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.18 77 virus, Serotype ‘A’ in buffaloes and cattle at 8 weeks Post Vaccination Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.19 78 virus, Serotype ‘A’ in buffaloes and cattle at 10 weeks Post Vaccination Comparative Geometric Mean Titers and Cumulative Mean Titers of 4.20 79 different vaccines against serotype ‘A’ of Foot and Mouth Disease virus Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.21 80 virus, Serotype ‘Asia I’ in buffaloes and cattle at 2 weeks Post Vaccination Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.22 81 virus, Serotype ‘Asia I’ in buffaloes and cattle at 4 weeks Post Vaccination Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.23 82 virus, Serotype ‘Asia I’ in buffaloes and cattle at 6 weeks Post Vaccination Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.24 83 virus, Serotype ‘Asia I’ in buffaloes and cattle at 8 weeks Post Vaccination Indirect Hemagglutination antibody titers against Foot and Mouth Disease 4.25 virus, Serotype ‘Asia I’ in buffaloes and cattle at 10 weeks Post 84 Vaccination Comparative Geometric Mean Titers and Cumulative Mean Titers of 4.26 different vaccines against serotype ‘Asia I’ of Foot and Mouth Disease 85 virus 6 LIST OF FIGURES No. Title Page No. Map of Punjab, showing three district selected for 3.1 23 seroprevalence of Foot and Mouth Disease Detection of seroprevalence of Foot and Mouth Disease with 4.1 54 Non Structural Protein- Enzyme Linked Immunosorbent Assay 4.2 Symptoms of Foot and Mouth Disease infected cattle 56 4.3 Clinical signs of Foot and Mouth Disease infected cattle 56 DNA amplified product (328 bp) of Foot and Mouth Disease 4.4 58 virus using universal primers DNA amplified product (1301 bp) of Foot and Mouth Disease 4.5 59 virus using serotype specific primers DNA amplified product (923 bp) of Foot and Mouth Disease 4.6 60 virus using serotype specific primers Detection of serotypes of Foot and Mouth Disease with Indirect 4.7 64 Sandwich Enzyme Linked Immunosorbent Assay Detection of antibody titers of Foot and Mouth Disease vaccines 4.8 67 with Indirect Haemagglutination test 7 CHAPTER – 1 INTRODUCTION Pakistan is an agricultural country and livestock have a great importance in its economy. Every 5th family in Pakistan is engaged directly or indirectly in livestock farming. It accounts for about 11.9% of total GDP and 55.4 % of agricultural value added. Total population of buffaloes and cattle in Pakistan is 33.7 million and 38.3 million respectively among which the major part (>61% of buffalo and >43% of cattle population) is residing in Punjab. Livestock is a major source of milk, meat, wool, hair, bones, fats, blood, hides and skins. Total 39945 thousand tons milk production and 1829 thousand tons beef production was recorded during the year 2012-2013 (Economic Survey of Pakistan, 2013).
Recommended publications
  • Download Map (PDF | 4.45
    ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! Overview - PUNJAB ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! K.P. ! ! ! ! ! ! Murree ! Tehsil ! ! ! ! ! ! Hasan Abdal Tehsil ! Attock Tehsil ! Kotli Sattian Tehsil ! Taxila Tehsil ! ! ! ! ! ! Attock ! ! ! ! Jand Tehsil ! Kahuta ! Fateh Jang Tehsil Tehsil ! ! ! Rawalpindi Tehsil ! ! ! Rawalpindi ! ! Pindi Gheb Tehsil ! J A M M U A N D K A S H M I R ! ! ! ! Gujar Khan Tehsil ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! Sohawa ! Tehsil ! ! ! A F G H A N I S T A N Chakwal Chakwal ! ! ! Tehsil Sarai Alamgir Tehsil ! ! Tala Gang Tehsil Jhelum Tehsil ! Isakhel Tehsil ! Jhelum ! ! ! Choa Saidan Shah Tehsil Kharian Tehsil Gujrat Mianwali Tehsil Mianwali Gujrat Tehsil Pind Dadan Khan Tehsil Sialkot Tehsil Mandi Bahauddin Tehsil Malakwal Tehsil Phalia Mandi Bahauddin Tehsil Sialkot Daska Tehsil Khushab Piplan Tehsil Tehsil Wazirabad Tehsil Pasrur Tehsil Narowal Shakargarh Tehsil Shahpur Tehsil Khushab Gujranwala Bhalwal Hafizabad Tehsil Gujranwala Tehsil Tehsil Narowal Tehsil Sargodha Kamoke Tehsil Kalur Kot Tehsil Sargodha Tehsil Hafizabad Nowshera Virkan Tehsil Pindi Bhattian Tehsil Noorpur Sahiwal Tehsil Tehsil Darya Khan Tehsil Sillanwali Tehsil Ferozewala Tehsil Safdarabad Tehsil Sheikhupura Tehsil Chiniot
    [Show full text]
  • Muzaffargarh
    ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! !! ! ! ! ! ! Overview - Muzaffargarh ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! ! Bhattiwala Kherawala !Molewala Siwagwala ! Mari PuadhiMari Poadhi LelahLeiah ! ! Chanawala ! ! ! ! ! ! ! Ladhranwala Kherawala! ! ! ! Lerah Tindawala Ahmad Chirawala Bhukwala Jhang Tehsil ! ! ! ! ! ! ! Lalwala ! Pehar MorjhangiMarjhangi Anwarwal!a Khairewala ! ! ! ! ! ! ! ! ! Wali Dadwala MuhammadwalaJindawala Faqirewala ! ! ! ! ! ! ! ! ! MalkaniRetra !Shah Alamwala ! Bhindwalwala ! ! ! ! ! Patti Khar ! ! ! Dargaiwala Shah Alamwala ! ! ! ! ! ! Sultanwala ! ! Zubairwa(24e6)la Vasawa Khiarewala ! ! ! ! ! ! ! Jhok Bodo Mochiwala PakkaMochiwala KumharKumbar ! ! ! ! ! ! Qaziwala ! Haji MuhammadKhanwala Basti Dagi ! ! ! ! ! Lalwala Vasawa ! ! ! Mirani ! ! Munnawala! ! ! Mughlanwala ! Le! gend ! Sohnawala ! ! ! ! ! Pir Shahwala! ! ! Langanwala ! ! ! ! Chaubara ! Rajawala B!asti Saqi ! ! ! ! ! ! ! ! ! BuranawalaBuranawala !Gullanwala ! ! ! ! ! Jahaniawala ! ! ! ! ! Pathanwala Rajawala Maqaliwala Sanpalwala Massu Khanwala ! ! ! ! ! ! Bhandniwal!a Josawala ! ! Basti NasirBabhan Jaman Shah !Tarkhanwala ! !Mohanawala ! ! ! ! ! ! ! ! ! ! Basti Naseer Tarkhanwala Mohanawala !Citiy / Town ! Sohbawala ! Basti Bhedanwala ! ! ! ! ! ! Sohaganwala Bhurliwala ! ! ! ! Thattha BulaniBolani Ladhana Kunnal Thal Pharlawala ! ! ! ! ! ! ! ! ! ! ! Ganjiwala Pinglarwala Sanpal Siddiq Bajwa ! ! ! ! ! Anhiwala Balochanwala ! Pahrewali ! ! Ahmadwala ! ! !
    [Show full text]
  • Crop Damage Assessment Along the Indus River
    0 1 0 2 K t A 0 s -P . u 1 2 g 4 n 1 u 0 io A 0 rs 0 -0 e 2 0 V 1 0 -2 L F " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " "" " " " " " " " " " " " " " " " " " " " " " " " " !( " " " " " " " " " " " " " " " " " " " !(" " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " "" " " " " " " " " " " " " " " " " " " " " " " " p " " " " " " " p " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " "" " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " : " " " " " " " !( " " " " " " " " " " " y " " " " " " " " " " " " " " " " " " " " " " " " " b " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " " d " " " " " " " " " " " " " !( " " e " " " " " " " " " " t " "" " "p " " " " " " " " " " r " !( " " " " !( " " " " !( " " " p " " " " " " " o " " " " " " " " " " " " " " " " " " " " " " " " " p " " " " " " " " " " " " " " " " " " " " p " " " " " "" " " " " " " " " " " " " " " " " " " u " " " " " " " " " " t " " " !( " " " S " " " . " " " " " " " " " " o " " " " " " n " " " " " " " " " " " " " " D" " p " " " nn " " " " " " " " " !( " " " " e " " " " " " " " " " " " r O " " " " " " " " " " " " " " " " " " a M " " " " " " " " " " I " " " " " " " " " " " " " " " " " " " e " " " " " " " " " aa " " " !( !(r C " " " " " " " " I " " " " " " " " " " " " " " " e " " " " " " " " " e L " " " p" " " " " " " " h I " " " " " " " " " tt ""
    [Show full text]
  • Reference Map
    Attock ‐ Reference Map Attock Tehsil Hasan Abdal Tehsil Punjab Fateh Jang Tehsil Jand Tehsil Pindi Gheb Tehsil Disclaimers: The designations employed and the presentation of material on this map do not imply the expression of any opinion whatsoever on the part of the Secretariat of the United Nations concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. Dotted line represents approximately the Line of Control in Jammu and Kashmir agreed upon by India and Pakistan. The final status of Jammu and Kashmir has not yet been agreed upon by the parties. Bahawalnagar‐ Reference Map Minchinabad Tehsil Bahawalnagar Tehsil Chishtian Tehsil Punjab Haroonabad Tehsil Fortabbas Tehsil Disclaimers: The designations employed and the presentation of material on this map do not imply the expression of any opinion whatsoever on the part of the Secretariat of the United Nations concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. Dotted line represents approximately the Line of Control in Jammu and Kashmir agreed upon by India and Pakistan. The final status of Jammu and Kashmir has not yet been agreed upon by the parties. p Bahawalpur‐ Reference Map Hasilpur Tehsil Khairpur Tamewali Tehsil Bahawalpur Tehsil Ahmadpur East Tehsil Punjab Yazman Tehsil Disclaimers: The designations employed and the presentation of material on this map do not imply the expression of any opinion whatsoever on the part of the Secretariat of the United Nations concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries.
    [Show full text]
  • Puma Energy Pakistan (Private) Limited
    11 Puma Energy Pakistan (Private) Limited Pumps Retail Prices of HSD and PMG Effective from 01st August, 2021 Pump Price Rs / Liter S.No Customer Name Address City HSD PMG 1 A. K. MANN PETROLEUM Chak # 112/7R, T. T. Singh-Burewala Road Toba Taik Singh 117.58 120.80 2 A.A ENTERPRISES SHAHPUR JAHANIAN TEHSIL MORO DISTRICT Daulatpur 117.05 120.29 3 A.Q MALIKANI PETROLEUM SERVICES Survey No.32 Deh Sattar Dino Shah,Tapo Mullan Dist Thatta 117.75 120.96 4 A.S PETROLEUM SERVICE Mouza Naroki Majaha, Pattoki-Changa Manga Road Pattoki 117.48 120.70 At Aray Wala Mouza Pakki (Near Qasba Shah Sadar Din)KM 26/27 From Dera Gazi Khan, Tunsaroad Indus 5 AADIL PETROLEUM SERVICES Dera Ghazi Khan 117.05 120.29 N-55 6 ABBAS FILLING STATION Block # 165/3 & 4, Sub #3A-3B-4A & 4B, Deh 86Nasrat Main Sanghar Road Nawabshah 117.05 120.29 7 ABBAS PETROLEUM STATION Kharian Dinga Road Noonan Wali Distt GujratNoonan Wali Noonan Wali - Gujrat 117.05 120.29 8 Abbasi Trucking Station Moro Servery No. 475, Situated in Deh Moro & Taluka Mo Moro 117.05 120.29 9 ABBASIA FILLING STATION Taluka Gularchi Plot No.A3 Khi Road, Shaheed Fazil Badin 117.93 121.12 10 ABDUL RAFAY PETROLEUM Mouza Kotla Qasim Khan Dinga Road, Tehsil Lala Mou Gujrat 117.05 120.29 11 ABDUL SATTAR PETROLEUM SERVICE Mouza Manga Ottar, (Near Honda Factory), on Nationon National Highway (N-5) (South Bond) Lahore 117.24 120.48 12 ABDULLAH PETROLEUM Chak Beli Khan / Rawalpindi Road, Tehsil & Distt Rawalpindi Rawal Pindi 117.05 120.29 13 ABDULLAH PETROLEUM STATION- GUJRAT Mouza Ganja B/W KM 8/9 From Lala Mopuza On NoonwalOn Noonwali-Ganja-Lala Mouza Road Gujrat 117.05 120.29 14 ABDULLAH PETROLEUM SERVICE Petrol Pump Baloch AJK, Baloch Sadhnoti AJK 117.48 120.70 15 ABID CNG STATION & PETROLEUM SERVICES On Jalal Pur Pirwala Road At Mouza KhaipurOpposite Al-Shamas At Shujanad Multan 117.16 120.40 16 ADIL FILLING STATION Survey No.
    [Show full text]
  • Population and Household Detail from Block to District Level
    POPULATION AND HOUSEHOLD DETAIL FROM BLOCK TO DISTRICT LEVEL PUNJAB (KHANEWAL DISTRICT) ADMIN UNIT POPULATION NO OF HH KHANEWAL DISTRICT 2,921,986 466,390 JAHANIAN TEHSIL 343,361 52,613 JAHANIAN MC 43,598 7010 CHARGE NO 03 43,598 7010 CIRCLE NO 01 10,417 1640 221030101 2,403 388 221030102 614 84 221030103 250 47 221030104 1,065 170 221030105 2,135 304 221030106 1,596 275 221030107 697 106 221030108 1,657 266 CIRCLE NO 02 4,001 655 221030201 751 113 221030202 480 86 221030203 781 116 221030204 658 121 221030205 613 94 221030206 718 125 CIRCLE NO 03 7,583 1226 221030301 2,625 432 221030302 237 43 221030303 521 105 221030304 776 126 221030305 503 84 221030306 2,921 436 CIRCLE NO 04 3,947 657 221030401 440 66 221030402 727 104 221030403 295 52 221030404 1,600 272 221030405 885 163 CIRCLE NO 05 9,034 1485 221030501 990 187 221030502 898 156 221030503 262 38 221030504 3,844 583 221030505 810 135 221030506 1,360 226 221030507 870 160 CIRCLE NO 06 8,616 1347 221030601 1,111 170 221030602 2,812 425 221030603 2,621 424 221030604 156 23 221030605 1,144 188 221030606 772 117 Page 1 of 74 POPULATION AND HOUSEHOLD DETAIL FROM BLOCK TO DISTRICT LEVEL PUNJAB (KHANEWAL DISTRICT) ADMIN UNIT POPULATION NO OF HH JAHANIAN QH 169,785 25814 055/10-R PC 5,480 875 CHAK NO 053/10-R 1,932 337 221011403 1,124 197 221011404 700 124 221011405 108 16 CHAK NO 054/10-R 2,037 318 221011402 1,133 188 221011406 904 130 CHAK NO 055/10-R 1,511 220 221011401 1,511 220 056/10-R PC 6,093 890 CHAK NO 056/10-R 2,028 307 221011501 1,059 157 221011502 969 150 CHAK NO 057/10-R 2,786
    [Show full text]
  • S.R.O. No.---/2011.In Exercise Of
    PART II] THE GAZETTE OF PAKISTAN, EXTRA., JANUARY 9, 2021 39 S.R.O. No.-----------/2011.In exercise of powers conferred under sub-section (3) of Section 4 of the PEMRA Ordinance 2002 (Xlll of 2002), the Pakistan Electronic Media Regulatory Authority is pleased to make and promulgate the following service regulations for appointment, promotion, termination and other terms and conditions of employment of its staff, experts, consultants, advisors etc. ISLAMABAD SATURDAY, JANUARY 9, 2021 PART II Statutory Notifications (S. R. O.) GOVERNMENT OF PAKISTAN MINISTRY OF NATIONAL FOOD SECURITY AND RESEARCH NOTIFICATION Islamabad, the 6th January, 2021 S. R. O. (17) (I)/2021.—In exercise of the powers conferred by section 15 of the Agricultural Pesticides Ordinance, 1971 (II of 1971), and in supersession of its Notifications No. S.R.O. 947(I)/2002, dated the 23rd December, 2002, S.R.O. 1251 (I)2005, dated the 15th December, 2005, S.R.O. 697(I)/2005, dated the 28th June, 2006, S.R.O. 604(I)/2007, dated the 12th June, 2007, S.R.O. 84(I)/2008, dated the 21st January, 2008, S.R.O. 02(I)/2009, dated the 1st January, 2009, S.R.O. 125(I)/2010, dated the 1st March, 2010 and S.R.O. 1096(I), dated the 2nd November, 2010. The Federal Government is pleased to appoint the following officers specified in column (2) of the Table below of Agriculture Department, Government of the Punjab, to be inspectors within the local limits specified against each in column (3) of the said Table, namely:— (39) Price: Rs.
    [Show full text]
  • Audit Report on the Accounts of Tehsil Municipal Administrations Khanewal
    AUDIT REPORT ON THE ACCOUNTS OF TEHSIL MUNICIPAL ADMINISTRATIONS KHANEWAL AUDIT YEAR 2013-14 AUDITOR GENERAL OF PAKISTAN TABLE OF CONTENTS ABBREVIATIONS AND ACRONYMS ....................................................................... i PREFACE ................................................................................................................... ii EXECUTIVE SUMMARY ...........................................................................................iii SUMMARY TABLES AND CHARTS .....................................................................viii Table 1: Audit Work Statistics ......................................................................................viii Table 2: Audit Observations regarding Financial Management ...................................viii Table 3: Outcome Statistics ............................................................................................. ix Table 4: Table of Irregularities pointed out ..................................................................... x Table 5: Cost -Benefit ...................................................................................................... x CHAPTER-1 ................................................................................................................... 2 1. TEHSIL MUNICIPAL DMINISTRATIONS KHANEWAL ............................ 2 1.1 INTRODUCTION .............................................................................................. 2 1.1.1 Comments on Budget and Accounts (Variance Analysis) ................................
    [Show full text]
  • Puma Energy Pakistan (Private) Limited
    11 Puma Energy Pakistan (Private) Limited Pumps Retail Prices of HSD and PMG Effective from 16th July, 2021 Pump Price Rs / Liter S.No Customer Name Address City HSD PMG 1 A. K. MANN PETROLEUM Chak # 112/7R, T. T. Singh-Burewala Road Toba Taik Singh 117.58 119.08 2 A.A ENTERPRISES SHAHPUR JAHANIAN TEHSIL MORO DISTRICT Daulatpur 117.05 118.58 3 A.Q MALIKANI PETROLEUM SERVICES Survey No.32 Deh Sattar Dino Shah,Tapo Mullan Dist Thatta 117.74 119.24 4 A.S PETROLEUM SERVICE Mouza Naroki Majaha, Pattoki-Changa Manga Road Pattoki 117.48 118.99 At Aray Wala Mouza Pakki (Near Qasba Shah Sadar Din)KM 26/27 From Dera Gazi Khan, Tunsaroad Indus 5 AADIL PETROLEUM SERVICES Dera Ghazi Khan 117.05 118.58 N-55 6 ABBAS FILLING STATION Block # 165/3 & 4, Sub #3A-3B-4A & 4B, Deh 86Nasrat Main Sanghar Road Nawabshah 117.05 118.58 7 ABBAS PETROLEUM STATION Kharian Dinga Road Noonan Wali Distt GujratNoonan Wali Noonan Wali - Gujrat 117.05 118.58 8 Abbasi Trucking Station Moro Servery No. 475, Situated in Deh Moro & Taluka Mo Moro 117.05 118.58 9 ABBASIA FILLING STATION Taluka Gularchi Plot No.A3 Khi Road, Shaheed Fazil Badin 117.92 119.40 10 ABDUL RAFAY PETROLEUM Mouza Kotla Qasim Khan Dinga Road, Tehsil Lala Mou Gujrat 117.05 118.58 11 ABDUL SATTAR PETROLEUM SERVICE Mouza Manga Ottar, (Near Honda Factory), on Nationon National Highway (N-5) (South Bond) Lahore 117.24 118.76 12 ABDULLAH PETROLEUM Chak Beli Khan / Rawalpindi Road, Tehsil & Distt Rawalpindi Rawal Pindi 117.05 118.58 13 ABDULLAH PETROLEUM STATION- GUJRAT Mouza Ganja B/W KM 8/9 From Lala Mopuza On NoonwalOn Noonwali-Ganja-Lala Mouza Road Gujrat 117.05 118.58 14 ABDULLAH PETROLEUM SERVICE Petrol Pump Baloch AJK, Baloch Sadhnoti AJK 117.48 118.99 15 ABID CNG STATION & PETROLEUM SERVICES On Jalal Pur Pirwala Road At Mouza KhaipurOpposite Al-Shamas At Shujanad Multan 117.16 118.68 16 ADIL FILLING STATION Survey No.
    [Show full text]
  • Latest Trends in Enrolment June 2015
    Latest Trends in Enrolment June 2015 August 12, 2015 Agenda Detailed Nielsen analysis Observations from the field 1 Agenda Detailed Nielsen analysis Observations from the field 2 Enrolment for 5-9 year olds has increased by 1.1% School participation rate 5-9 year olds (%) 90.4% 89.3% 1.1% 88.2% 86.8% 86.1% 85.9% 84.8% Nov-11 Jun-12Nov-12 Jun-13 Nov-13 Nov-14 Jun-15 SOURCE: Nielsen Household Survey– November 2011 to June 2015 3 Enrolment ( Nov 2011 - Jun 2015) School participation rate – Urban Areas 5-9 year olds (%) 94.7% 92.9% 93.1% 93.1% 92.7% 92.6% 92.2% Nov-11 Jun-12 Nov-12 Jun-13 Nov-13 Nov-14 Jun-15 School participation rate – Rural Areas 5-9 year olds (%) 88.7% 87.8% 86.5% 84.4% 83.5% 83.2% 81.9% Nov-11 Jun-12 Nov-12 Jun-13 Nov-13 Nov-14 Jun-15 SOURCE: Nielsen Household Survey– November 2011 to June 2015 Margin of Error < 4% with a 95 % confidence interval 4 Enrolment has increased significantly over the last 4 years, with a proportionate increase observed for across all age groups Enrolment Percent Jun 2015 100% Nov 2011 90% 80% 70% 60% 50% 40% 30% 20% 10% 0% 0 4 5 6 7 8 9 10 11 12 13 14 15 16 Age No. of years SOURCE: Nielsen Household Survey November 2011; June 2015 Margin of Error < 3.1% with a 95 % confidence interval 5 35 districts in Punjab show improvement since November 2011 District % Change since November 2011 Rahim Yar Khan 15.4% Lodhran 13.1% Bahawalnagar 13.0% Bahawalpur 12.1% Pakpattan 10.9% Sheikhupura 9.9% <3.1 % Layyah 9.7% >3.1% Sahiwal 9.2% Mianwali 9.1% Sargodha 9.0% 25 districts show a % Khushab 8.2% change
    [Show full text]
  • Citizens' Campaigns for Women's Participation in Local Government Elections 2001 and 2005
    Citizens’ Campaigns for Women’s Participation in Local Government Elections 2001 and 2005 Backdrop, Glimpses of the Campaigns, Overall Results Aurat Publication and Information Service Foundation Contents The Beginning of the End............................................................................... v Acknowledgements......................................................................................... x Balochistan.............................................................................................................. 1 Awaran................................................................................................................ 3 Barkhan............................................................................................................... 7 Bolan................................................................................................................. 11 Chagai/Noshki................................................................................................... 14 Dera Bugti......................................................................................................... 22 Gwadar.............................................................................................................. 25 Jaffarabad.......................................................................................................... 29 Jhal Magsi ......................................................................................................... 34 Kalat.................................................................................................................
    [Show full text]
  • *Successful Students Are Advised to Check Their Particulars Mentioned Below I.E
    STUDENTS LOAN SCHEME – NAMES OF SUCCESSFUL STUDENTS FOR THE SESSION 2015-2016 ANNOUNCEMENT The State Bank of Pakistan's Apex Committee, a high powered body on Students Loan Scheme (SLS) has approved an amount of Rs.174.923 million (Against 14th Advertisement) of Interest free loan to 1,044 deserving students for their entire period of studies. These students have been pursuing their studies in different technical and professional disciplines leading to Graduation, Masters, M.Phil and Ph.D within Pakistan in public sector universities/colleges during the Session 2015-2016. Important :- Please Note that the current year disbursement has to be made within 45 days from the date of receipt of the Sanction Advice by the eligible student. Thereafter, no disbursement will be allowed without prior clearance of the Students Loan Wing, NBP, Head Office, Karachi. In case the student’s copy is misplaced/undelivered, the Principal/Registrar may provide photocopy of the “Sanction Advice” to the student to facilitate disbursement by the concerned Branch. IMPORTANT INSTRUCTIONS *Successful Students are advised to check their particulars mentioned below i.e. names, permanent & postal address, institutions, their CNIC numbers and their Domiciles. If there is any correction required they should send email with existing particulars or applicant’s eligibility reference No. to Mr. Shamim Iqbal, Senior Vice President, NBP at e-mail [email protected] within fifteen (15) days of this announcement. ** All those un-successful students who could not meet the qualification/eligibility criteria of the Students Loan Scheme are advised that if they are eager to know the assigned reason of their disqualification they may send e-mail at the above mentioned address.
    [Show full text]