Tips for Formulating with Steviol Glycosides

Tips for formulating with steviol glycosides

Targeting Sweetness Concentration Response Curve 8 Whether focused on sugar reduction, RA80 or RA95 transitioning from artificial high potency sweeteners (HPS) or development of a no-sugar 6 added product, understanding your target RA50 sweetness is the critical first step. Sweetness is 4 quantified in terms of sucrose equivalent value (SEV) and may be calculated using the equation below. The amount of TRUVIA® stevia leaf (%) Equivalent Sucrose 2 extract needed to replace the sweetness of the | | | | | missing sugar may then be extrapolated from a 0 100 200 300 400 500 concentration-response curve. Truvía® Stevia Leaf Extract Concentration (ppm)

Reduction in Sugar (g per serving) x100 Sucrose Equivalent Value SEV = Serving Size (g)

Guidelines for Bracketing Sweetness & Taste-Cost Optimization

2% 4% 6% sucrose equivalent sucrose equivalent sucrose equivalent <3%SE 3-6%SE >6%SE sweetness sweetness sweetness Taste Cost Taste Cost Taste Cost is approximately equal to RA80 Good Good Good Best Good Best RA95 Good Good Good

40-60 ppm 110-150 ppm 250-300 ppm RA50 Good Best Truvia® RA80 Truvia® RA80 Truvia® RA80

EU maximum use level 240 ppm RA80 (approximately 5.5% SEV)

Brought to You by Cargill Optimizing Sweetness

It is well established that HPS reach a maximum sweetness plateau. At higher concentrations, increasing use level yields little incremental sweetness and introduces undesirable off-tastes. In highly sweetened applications (ex. greater than 6-8% sucrose equivalent sweetness), it is often beneficial to blend with another sweetener, like Zerose® erythritol, sugar or other nutritive sweeteners. Zerose® erythritol may be used up to 3.5% in typical beverage applications. This level boosts sweetness intensity by 2% SE and improves sweetness quality. (See regulatory guidelines for specific country and applications) Tailoring the type and ratio of acids (citric, malic, lactic) can help achieve a better sweet/sour balance.

Blending To Boost Sweetness Blending With Sugars 12 12

10 10 6% 4% 8 8 2% Practical % 6 6 0 Formulation: Truvía® RA95/80 Percent Sugar Present 4 4 Sucrose Equivalent (%) Equivalent Sucrose Sucrose Equivalent (%) Equivalent Sucrose 2 2 Zerose® Erythritol | | | | | | | | | | 0 0 100 200 300 400 500 100 200 300 400 500 Truvía® RA95 or RA80 Concentration (ppm) Truvía® RA95 or RA80 Concentration (ppm)

Replacing Artificial HPS Sucrose Equivalent Comparison: Each HPS has unique taste character with respect to 15 1. Sucralose 2. Saccharin sweetness intensity, temporal profile and potential side-tastes 3. Truvia ® RA95/80 10 4. Aspartame The following figures are helpful in determining sucrose 5. Ace-K 1 equivalent sweetness when replacing artificial HPS with 2 ® 3 4 TRUVIA stevia leaf extract 5 5 Matching sweetness and flavor profile can be a difficult (%) Equivalent Sucrose exercise. 0 | | | 200 400 600 Sweetener Concentration (ppm)

Finishing Touches For more information 1-866-456-8872 Natural flavors, including sweetness enhancers and taste modifiers, cargillfoods.com/sweeteners can be valuable to round out sweetness and deliver the best taste.

References For all lines except RA95/80: DuBois GE, Walters DE, Schiffman SS, Warwick ZS, Booth BJ, Pecore SD, Gibes K, Carr BT, Brands LM. (1991) Concentration-response relationships of sweeteners. In: Walters DE, Orthoefer FT, DuBois GE, editors. ACS symposium series 450: Sweeteners. Discovery, molecular design and chemoreception. Washington, D.C.: American Chemical Society. pp. 261–76. For RA80: Cargill internal report For RA95: Fry JC, Yurttas N & Biermann KL (2011) The sweetness concentration-response behavior of rebiana at room and refrigerator temperatures. Journal of Food Science 76: S545–8. THE INFORMATION CONTAINED HEREIN IS BELIEVED TO BE TRUE AND CORRECT UNDER US LAW. ALL STATEMENTS, RECOMMENDATIONS OR SUGGESTIONS ARE MADE WITHOUT GUARANTEE, EXPRESS OR IMPLIED, AND ARE SUBJECT TO CHANGE WITHOUT NOTICE. WE DISCLAIM ALL WARRANTIES, EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND FREEDOM FROM INFRINGEMENT AND DISCLAIM ALL LIABILITY IN CONNECTION WITH THE USE OF THE PRODUCTS OR INFORMATION CONTAINED HEREIN.