DOCSLIB.ORG

(Amphipoda) Allows the Identification of a New Species, Jassa Cadetta Sp

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
(Amphipoda) Allows the Identification of a New Species, Jassa Cadetta Sp ARTICLE IN PRESS Organisms, Diversity & Evolution 8 (2008) 337–345 www.elsevier.de/ode A cytogenetical study of Ischyroceridae (Amphipoda) allows the identification of a new species, Jassa cadetta sp. n., in the Lagoon of Venice Traudl Krappa, Massimiliano Rampinb, Angelo Libertinib,Ã aForschungsinstitut Museum A. Koenig, Adenauerallee 160, 53113 Bonn, Germany bCNR-Institute of Marine Science, Riva 7 Martiri 1364/A, 30122 Venezia, Italy Received 20 November 2007; accepted 16 June 2008 Abstract Jassa cadetta sp. n. (Amphipoda: Ischyroceridae) is described from the Venice Lagoon, northern Adriatic Sea, and a key to Mediterranean members of the genus Jassa Leach is provided. The new species is separated from J. marmorata Holmes primarily by cytogenetics, differing in chromosome number (2n ¼ 10 in J. cadetta vs. 2n ¼ 12 in J. marmorata), karyotype morphology (FN ¼ 20 vs. FN ¼ 22), and chromosome location of 18S-5.8S-28S ribosomal cistrons. Cytogenetic analysis of Ischyrocerus anguipes Krøyer (2n ¼ 10, FN ¼ 18) gives a first insight into karyological diversity among Ischyroceridae. Analysis of random amplified polymorphic DNA markers confirms the distinction between J. cadetta sp. n. and J. marmorata. r 2008 Gesellschaft fu¨ r Biologische Systematik. Published by Elsevier GmbH. All rights reserved. Keywords: Amphipoda; Northern Adriatic Sea; Cytogenetics; PCR-RAPD; New species Introduction examines early embryos as material for karyotyping (Libertini et al. 2000); the body of the corresponding Libertini et al. (2000) published a monograph on the ovigerous female is usually preserved for species cytogenetics of Jassa marmorata Holmes, 1903 from the identification. One female from the Lido mouth, Venice Lagoon. The population was characterized by a identified as J. marmorata, possessed a karyotype clearly karyotype of 2n ¼ 12 chromosomes, with 5 pairs of different from that outlined above. This discrepancy was metacentrics and 1 pair of subtelocentrics. These data initially explained as a confusion in labeling of the were consistent with those from an Atlantic population female, because many other J. marmorata specimens of the species in which haploid and diploid chromosome with a ‘regular’ karyotype were found in the same numbers had been found to be 6 and 12, respectively sample. (Coleman 1994). In subsequent years, other fouling samples from the A fouling sample was collected from wooden piles at deepest part of blue mussel cultivation socks in the the northern mouth of the Venice Lagoon (Lido mouth) central mouth of the Venice Lagoon (Malamocco in 2000 and studied cytogenetically. Our lab regularly mouth) contained many Jassa specimens with a karyo- type different from that of J. marmorata, compelling ÃCorresponding author. Tel.: +39 041 2404711; the authors to propose the presence of another fax: +39 041 5204126. species, recently recognized as new, and described E-mail address: [email protected] (A. Libertini). below. 1439-6092/$ - see front matter r 2008 Gesellschaft fu¨ r Biologische Systematik. Published by Elsevier GmbH. All rights reserved. doi:10.1016/j.ode.2008.06.001 ARTICLE IN PRESS 338 T. Krapp et al. / Organisms, Diversity & Evolution 8 (2008) 337–345 The present paper reports on a genetic comparison room temperature (RT), once (5 min) in 2 Â SSC/0.1% between the two Jassa species from the Venice Lagoon, Tween20 at RT, and once (5 min) in PBS/0.1% based on karyotypes and random amplified poly- Tween/0.5% skimmed milk powder at RT. Hybridi- morphic DNA (PCR-RAPD), and provides the descrip- zation signals were detected with FITC-conjugated tion of Jassa cadetta sp. n. The cytogenetic comparison antidigoxigenin (Roche Molecular Biochemicals) or Cy3- is extended to Ischyrocerus anguipes Krøyer, 1838, conjugated extravidin (Sigma). Slides were mounted in considered here as an outgroup belonging to the same AF1 antifade solution (Citifluor) containing 2 mg/ml tribe, Ischyrocerini, according to the classification DAPI (40,6-diamidino-2-phenylindole). scheme by Myers and Lowry (2003). Observations were made with a JenaMed 2-fluores- cence microscope (Carl Zeiss Jena, Germany) equipped with the 410/450 and the 510/570 filter sets. Normal light and fluorescence images were taken with a Canon Material and methods EOS 10D digital camera, processed and merged with Adobe Photoshop Elements 2.0. The Jassa specimens used in this study were collected For PCR-RAPD analysis, genomic DNA was ex- at different sites in the Venice Lagoon (NE Italy). tracted and purified from 10 males of J. marmorata and Ischyrocerus anguipes was collected along the shores 6 males of J. cadetta sp. n. with the GenElute near the Sandgerji Marine Centre of the University of Mammalian Genomic Miniprep kit (SIGMA). The Iceland (SW Iceland), during low tide. PCR-RAPD protocol was performed following Costa Chromosome preparations were made with the et al. (2004) with slight modifications, using the PCR hot-dry method applied to early embryos as described ReadyMix kit (Sigma). PCR reactions were performed by Libertini et al. (2000). The body of the mother in a final volume of 25 ml, containing 0.2 mM each of carrying the embryos was preserved in 70% ethanol for dNTP, 0.75 U of Taq DNA polymerase, 1 Â PCR buffer identification. (20 mM Tris–HCl, pH 8.3; 50 mM KCl), 2.5 mM MgCl2, For conventional karyotyping, slides were stained 0.8 mM primer, and 20 ng template DNA. Primers were for 10 min in 5% Giemsa solution in phosphate buffer chosen from those successfully used by Costa et al. (pH 6.8). At least 100 chromosome plates for each (2004) for Gammarus locusta; their respective names species were counted to determine the diploid chromo- and sequences were (50-to-30): A2 (TGCCGAGCTG), some number. Karyotypes were arranged according to A9 (GGGTAACGCC), A10 (GTGATCGCAG), A16 chromosome size and shape using digitized pictures of (AGCCAGCGAA), D2 (GGACCCAACC), D3 (GTC metaphase figures edited using Corel PhotoPaint. GCCGTCA), D5 (TGAGCGGACA), D7 (TTGGCA Chromosome classification is according to Levan et al. CGGG). All primers were purchased from MWG (1964); the centromeric index was evaluated following Biotech. Cycling conditions consisted of initial dena- Naranjo et al. (1983). At least 20 karyotypes were turation at 95 1C for 5 min, followed by 40 amplification examined for each species. cycles (denaturation at 93 1C for 40 s, annealing at 37 1C The chromosomal localization of major (18S-5.8S- for 60 s, extension at 72 1C for 60 s), and by a final 28S) and minor (5S) rDNA sequences was performed by extension for 6 min at 72 1C. DNA was then precipitated means of fluorescent in situ hybridization (FISH). from the aqueous phase with ethanol and stored at A probe containing 18S-5.8S-28S genes plus intergenic À20 1C for at least 2 h or overnight. The samples spacer of the fruit fly Drosophila melanogaster (pDm238; were then washed in 70% ethanol. The amplification Roiha et al. 1981) was used for major rDNA FISH. As products were maintained at 4 1C until being loaded the probe for 5S rDNA FISH, genomic DNA of onto the gels. Electrophoresis was conducted on J. marmorata was amplified by PCR, using two primers 2.5% agarose gels in TAE buffer (40 mM Tris, (50-GAAAGCACCACTTCTCGTCC-30 and 50-AACG pH 7.6; 20 mM acetic acid, 1 mM EDTA), run at 90 V TGGTATGGCCGTTGAC-30) obtained from the 5S for 2.5 h or for 7 cm. The gels were stained in an rRNA sequence of the isopod Proasellus coxalis ethidium bromide solution (30 mg/l), then photo- (Dollfus, 1892) (Pelliccia et al. 1998). Probes were graphed under UV light with a Canon EOS 10D digital labeled by nick translation with digoxigenin-11-dUTP camera. A molecular size standard consisting of or biotin-14-dATP (Roche Molecular Biochemicals or 50–3000 bp ‘‘direct load’’ ladder (Sigma) was run in Invitrogen). lanes flanking the samples, and a negative control in Chromosomes were denatured for 4 min in 70% each gel. formamide/2 Â SSC at 69 1C. Denaturation of the For the morphological description of Jassa cadetta probes was performed for 10 min at 75 1C. Hybridiza- sp. n., the typical procedure for amphipods was used: direct tion was allowed to proceed overnight at 37 1C. Slides observation of specimens in alcohol and glycerine under were washed three times in 50% formamide/2 Â SSC Reichert and Wild M5 dissecting microscopes; dissec- at 40 1C (5 min each), twice (5 min) in 2 Â SSC at tion, slide preparation (and storage) in glycerine or ARTICLE IN PRESS T. Krapp et al. / Organisms, Diversity & Evolution 8 (2008) 337–345 339 Faure’s medium; then drawing under a Wild M20 et al. 2000; shown in Fig. 2A, for colour version see the microscope. online edition of this paper) and in I. anguipes (Fig. 2C). In J. cadetta with 2n ¼ 10, 18S-5.8S-28S rDNA cistrons were located at the end of the shorter arm in the second- Results and discussion longest metacentric pair (Fig. 2B). Therefore, in I. anguipes and J. cadetta nucleolus organizer regions (NORs) correspond to the secondary constrictions Genetics previously observed in Giemsa-stained chromosomes. The 5S rDNA probes hybridized in an intercalate According to Libertini et al. (2000), J. marmorata region of the longest arm of an average-sized metacentric from the Venice Lagoon has a karyotype (Fig. 1A) pair in all three species studied (Fig. 2D–F). In 6 of 7 composed of 5 pairs of metacentric chromosomes examined metaphase figures of I. anguipes an additional (pairs 1–5) and 1 pair of subtelocentrics (pair 6) much metacentric chromosome had positive intercalate FISH shorter than the other elements. The diploid chromo- signals (Fig. 2D–F). 18S-5.8S-28S rDNA and 5S rDNA some number (2n) is 12, the fundamental number of cistrons are localized in two different chromosome pairs in chromosome arms (FN) is 22.
Load more

Recommended publications
  • Species of the Genus Ericthonius (Crustacea: Amphipoda: Ischyroceri- Dae) from Western Japan with Description of a New Species
    Bull. Natl. Mus. Nat. Sci., Ser. A, Suppl. 3, pp. 15–36, March 22, 2009 Species of the Genus Ericthonius (Crustacea: Amphipoda: Ischyroceri- dae) from Western Japan with Description of a New Species Hiroyuki Ariyama Marine Fisheries Research Center, Research Institute of Environment, Agriculture and Fisheries, Osaka Prefectural Government, Tanagawa, Misaki, Osaka, 599–0311 Japan E-mail: [email protected] Abstract Two species of the genus Ericthonius Milne Edwards, 1830 (Amphipoda: Ischyroceri- dae), E. convexus sp. nov. and E. pugnax Dana, 1852, are reported from western Japan. Both species share a posterodistal lobe on the article 2 in male pereopod 5, which only E. pugnax pos- sesses in all the Ericthonius species described. The new species is very similar to the latter species in the morphological characters and the general coloration in life. However, the new species is dis- tinguishable from the latter species by the relatively larger eyes, the relatively slender peduncles of antennae, the convex posterior edge of telson between spinulose lobes (straight or concave in E. pugnax), and the distal edge of article 6 of gnathopod 2 in the hyper-adult male having a distinct incision (such an incision is absent in E. pugnax). In addition, the habitat preference is also differ- ent between the two species; E. convexus occurs on sandy-mud bottom, whereas E. pugnax inhab- its under rocks in the intertidal zone and among seaweeds in the shallow sea. Key words : Amphipoda, Ischyroceridae, Ericthonius, new species, Japan. The ischyrocerid genus Ericthonius was estab- Suo-nada in the Seto Inland Sea and Ariake Sea, lished by Milne Edwards (1830) with E.
    [Show full text]
  • 1 Genetic Diversity in Two Introduced Biofouling Amphipods
    1 Genetic diversity in two introduced biofouling amphipods (Ampithoe valida & Jassa 2 marmorata) along the Pacific North American coast: investigation into molecular 3 identification and cryptic diversity 4 5 Erik M. Pilgrim and John A. Darling 6 US Environmental Protection Agency 7 Ecological Exposure Research Division 8 26 W. Martin Luther King Drive, Cincinnati, OH 45268, USA. 9 10 Running Title: Genetic diversity of introduced Ampithoe and Jassa 11 12 Article Type: Biodiversity Research 13 14 ABSTRACT 15 Aim We investigated patterns of genetic diversity among invasive populations of A. valida and J. 16 marmorata from the Pacific North American coast to assess the accuracy of morphological 17 identification and determine whether or not cryptic diversity and multiple introductions 18 contribute to the contemporary distribution of these species in the region. 19 Location Native range: Atlantic North American coast; Invaded range: Pacific North American 20 coast. 21 Methods We assessed indices of genetic diversity based on DNA sequence data from the 22 mitochondrial cytochrome c oxidase subunit I (COI) gene, determined the distribution of COI 23 haplotypes among populations in both the invasive and putative native ranges of A. valida and J. 24 marmorata, and reconstructed phylogenetic relationships among COI haplotypes using both 25 maximum parsimony and Bayesian approaches. 26 Results Phylogenetic inference indicates that inaccurate species level identifications by 27 morphological criteria are common among Jassa specimens. In addition, our data reveal the 28 presence of three well supported but previously unrecognized clades of A. valida among 29 specimens in the northeastern Pacific. Different species of Jassa and different genetic lineages of 1 30 Ampithoe exhibit striking disparity in geographic distribution across the region as well as 31 substantial differences in genetic diversity indices.
    [Show full text]
  • Additions to and Revisions of the Amphipod (Crustacea: Amphipoda) Fauna of South Africa, with a List of Currently Known Species from the Region
    Additions to and revisions of the amphipod (Crustacea: Amphipoda) fauna of South Africa, with a list of currently known species from the region Rebecca Milne Department of Biological Sciences & Marine Research Institute, University of CapeTown, Rondebosch, 7700 South Africa & Charles L. Griffiths* Department of Biological Sciences & Marine Research Institute, University of CapeTown, Rondebosch, 7700 South Africa E-mail: [email protected] (with 13 figures) Received 25 June 2013. Accepted 23 August 2013 Three species of marine Amphipoda, Peramphithoe africana, Varohios serratus and Ceradocus isimangaliso, are described as new to science and an additional 13 species are recorded from South Africa for the first time. Twelve of these new records originate from collecting expeditions to Sodwana Bay in northern KwaZulu-Natal, while one is an introduced species newly recorded from Simon’s Town Harbour. In addition, we collate all additions and revisions to the regional amphipod fauna that have taken place since the last major monographs of each group and produce a comprehensive, updated faunal list for the region. A total of 483 amphipod species are currently recognized from continental South Africa and its Exclusive Economic Zone . Of these, 35 are restricted to freshwater habitats, seven are terrestrial forms, and the remainder either marine or estuarine. The fauna includes 117 members of the suborder Corophiidea, 260 of the suborder Gammaridea, 105 of the suborder Hyperiidea and a single described representative of the suborder Ingolfiellidea.
    [Show full text]
  • The 17Th International Colloquium on Amphipoda
    Biodiversity Journal, 2017, 8 (2): 391–394 MONOGRAPH The 17th International Colloquium on Amphipoda Sabrina Lo Brutto1,2,*, Eugenia Schimmenti1 & Davide Iaciofano1 1Dept. STEBICEF, Section of Animal Biology, via Archirafi 18, Palermo, University of Palermo, Italy 2Museum of Zoology “Doderlein”, SIMUA, via Archirafi 16, University of Palermo, Italy *Corresponding author, email: [email protected] th th ABSTRACT The 17 International Colloquium on Amphipoda (17 ICA) has been organized by the University of Palermo (Sicily, Italy), and took place in Trapani, 4-7 September 2017. All the contributions have been published in the present monograph and include a wide range of topics. KEY WORDS International Colloquium on Amphipoda; ICA; Amphipoda. Received 30.04.2017; accepted 31.05.2017; printed 30.06.2017 Proceedings of the 17th International Colloquium on Amphipoda (17th ICA), September 4th-7th 2017, Trapani (Italy) The first International Colloquium on Amphi- Poland, Turkey, Norway, Brazil and Canada within poda was held in Verona in 1969, as a simple meet- the Scientific Committee: ing of specialists interested in the Systematics of Sabrina Lo Brutto (Coordinator) - University of Gammarus and Niphargus. Palermo, Italy Now, after 48 years, the Colloquium reached the Elvira De Matthaeis - University La Sapienza, 17th edition, held at the “Polo Territoriale della Italy Provincia di Trapani”, a site of the University of Felicita Scapini - University of Firenze, Italy Palermo, in Italy; and for the second time in Sicily Alberto Ugolini - University of Firenze, Italy (Lo Brutto et al., 2013). Maria Beatrice Scipione - Stazione Zoologica The Organizing and Scientific Committees were Anton Dohrn, Italy composed by people from different countries.
    [Show full text]
  • New Insights from the Neuroanatomy of Parhyale Hawaiensis
    bioRxiv preprint doi: https://doi.org/10.1101/610295; this version posted April 18, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. The “amphi”-brains of amphipods: New insights from the neuroanatomy of Parhyale hawaiensis (Dana, 1853) Christin Wittfoth, Steffen Harzsch, Carsten Wolff*, Andy Sombke* Christin Wittfoth, University of Greifswald, Zoological Institute and Museum, Dept. of Cytology and Evolutionary Biology, Soldmannstr. 23, 17487 Greifswald, Germany. https://orcid.org/0000-0001-6764-4941, [email protected] Steffen Harzsch, University of Greifswald, Zoological Institute and Museum, Dept. of Cytology and Evolutionary Biology, Soldmannstr. 23, 17487 Greifswald, Germany. https://orcid.org/0000-0002-8645-3320, sharzsch@uni- greifswald.de Carsten Wolff, Humboldt University Berlin, Dept. of Biology, Comparative Zoology, Philippstr. 13, 10115 Berlin, Germany. http://orcid.org/0000-0002-5926-7338, [email protected] Andy Sombke, University of Vienna, Department of Integrative Zoology, Althanstr. 14, 1090 Vienna, Austria. http://orcid.org/0000-0001-7383-440X, [email protected] *shared last authorship ABSTRACT Background Over the last years, the amphipod crustacean Parhyale hawaiensis has developed into an attractive marine animal model for evolutionary developmental studies that offers several advantages over existing experimental organisms. It is easy to rear in laboratory conditions with embryos available year- round and amenable to numerous kinds of embryological and functional genetic manipulations. However, beyond these developmental and genetic analyses, research on the architecture of its nervous system is fragmentary.
    [Show full text]
  • Hard Bottom Substrate Monitoring Horns Rev Offshore Wind Farm
    Engineering Hard Bottom Substrate Monitoring Horns Rev Offshore Wind Farm Annual Status Report 2004 v 'TW- ELsam Engineering Hard Bottom Substrate Monitoring Horns Rev Offshore Wind Farm Annual Status Report 2004 Published: 2. May 2005 Prepared: Simon B. Leonhard Editing: Gitte Spanggaard John Pedersen Checked: Bjame Moeslund Artwork: Kirsten Nygaard Approved: Simon B. Leonhard Cover photos: Jens Christensen Simon B. Leonhard Photos: Jens Christensen Maks Klaustmp Simon B. Leonhard English review: Matthew Cochran © No part of this publication may be reproduced by any means without clear reference to the source. Homs Rev. Hard Bottom Substrate Monitoring Page 3 Annual Status Report 2004 TABLE OF CONTENTS PAGE Summary.................................................................................................................................... 4 Sammenfatning (in Danish) ....................................................................................................... 7 1. Introduction ......................................................................................................................... 10 2. Methodology....................................................................................................................... 11 2.1. The research area...........................................................................................................11 2.2. Field activities................................................................................................................13 2.3. Test fishing..................................................................................................................
    [Show full text]
  • Amphipod Newsletter 39 (2015)
    AMPHIPOD NEWSLETTER 39 2015 Interviews BIBLIOGRAPHY THIS NEWSLETTER PAGE 19 FEATURES INTERVIEWS WITH ALICJA KONOPACKA AND KRZYSZTOF JAŻDŻEWSKI PAGE 2 MICHEL LEDOYER WORLD AMPHIPODA IN MEMORIAM DATABASE PAGE 14 PAGE 17 AMPHIPOD NEWSLETTER 39 Dear Amphipodologists, Statistics from We are delighted to present to you Amphipod Newsletter 39! this Newsletter This issue includes interviews with two members of our amphipod family – Alicja Konopacka and Krzysztof Jazdzewski. Both tell an amazing story of their lives and work 2 new subfamilies as amphipodologists. Sadly we lost a member of our amphipod 21 new genera family – Michel Ledoyer. Denise Bellan-Santini provides us with a fitting memorial to his life and career. Shortly many 145 new species members of the amphipod family will gather for the 16th ICA in 5 new subspecies Aveiro, Portugal. And plans are well underway for the 17th ICA in Turkey (see page 64 for more information). And, as always, we provide you with a Bibliography and index of amphipod publications that includes citations of 376 papers that were published in 2013-2015 (or after the publication of Amphipod Newsletter 38). Again, what an amazing amount of research that has been done by you! Please continue to notify us when your papers are published. We hope you enjoy your Amphipod Newsletter! Best wishes from your AN Editors, Wim, Adam, Miranda and Anne Helene !1 AMPHIPOD NEWSLETTER 39 2015 Interview with two prominent members of the “Polish group”. The group of amphipod workers in Poland has always been a visible and valued part of the amphipod society. They have organised two of the Amphipod Colloquia and have steadily provided important results in the world of amphipod science.
    [Show full text]
  • Zootaxa, Description of Two New Species of Ischyroceridae
    Zootaxa 1857: 55–65 (2008) ISSN 1175-5326 (print edition) www.mapress.com/zootaxa/ ZOOTAXA Copyright © 2008 · Magnolia Press ISSN 1175-5334 (online edition) Description of two new species of Ischyroceridae (Crustacea: Amphipoda) from the coast of Southeastern Brazil MARIA TERESA VALÉRIO-BERARDO,1 ANA MARIA THIAGO DE SOUZA,1 & CARINA WAITEMAN RODRIGUES2 1Universidade Presbiteriana Mackenzie, CCBS, Rua da Consolação 896, CEP 01302-907, São Paulo, SP, Brasil. E-mail: [email protected] 2Universidade de São Paulo, Instituto Oceanográfico, Praça do Oceanográfico, 191, 05508-900, São Paulo, SP, Brasil Abstract Taxonomic descriptions and figures are provided for 2 new species of Ischyroceridae (Cerapus jonsoni sp. nov. and Notopoma fluminense sp. nov.) from samples of Southwestern Atlantic, between the latitudes 22° and 24° S. Cerapus jonsoni sp. nov. is easily distinguished from others species of the genera recorded in Atlantic Ocean by dacyli of pereo- pods 6 and 7 with 1 accessory spine. Notopoma fluminense sp. nov. is characterized by 5 setal teeth on outer plate of maxilla 1, only 1 accessory spine on pereopod 7 dactylus and pleopod 3 absent. Key words: Cerapus jonsoni sp. nov., Notopoma fluminense sp. nov., Amphipoda, Ischyroceridae, taxonomy Introduction The tubiculous genus Cerapus has been subject to revision by Lowry & Berents, 1996, when it was estab- lished 5 genera in the Cerapus clade (Bathypoma Lowry & Berents, 1996; Cerapus Say, 1817; Notopoma Lowry & Berents, 1996; Runanga Barnard, 1961 and Paracerapus Budnikova, 1989). The current study fol- lows this taxonomic approach. The genus Notopoma can be distinguished from Cerapus by having a peduncu- lar article 1 of antenna 1 dorsally and medially produced, which functions as an operculum for closing the tube.
    [Show full text]
  • 1 Amphipoda of the Northeast Pacific (Equator to Aleutians, Intertidal to Abyss): IX. Photoidea
    Amphipoda of the Northeast Pacific (Equator to Aleutians, intertidal to abyss): IX. Photoidea - a review Donald B. Cadien, LACSD 22 July 2004 (revised 21 May 2015) Preface The purpose of this review is to bring together information on all of the species reported to occur in the NEP fauna. It is not a straight path to the identification of your unknown animal. It is a resource guide to assist you in making the required identification in full knowledge of what the possibilities are. Never forget that there are other, as yet unreported species from the coverage area; some described, some new to science. The natural world is wonderfully diverse, and we have just scratched its surface. Introduction to the Photoidea Over more than a century the position of the photids has been in dispute. Their separation was recommended by Boeck (1871), a position maintained by Stebbing (1906). Others have relegated the photids to the synonymy of the isaeids, and taxa considered here as photids have been listed as members of the Family Isaeidae in most west coast literature (i.e. J. L. Barnard 1969a, Conlan 1983). J. L. Barnard further combined both families, along with the Aoridae, into an expanded Corophiidae. The cladistic examination of the corophioid amphipods by Myers and Lowry (2003) offered support to the separation of the photids from the isaeids, although the composition of the photids was not the same as viewed by Stebbing or other earlier authors. The cladistic analysis indicated the Isaeidae were a very small clade separated at superfamily level from the photids, the neomegamphopids, and the caprellids within the infraorder Caprellida.
    [Show full text]
  • Comments on Corophi
    Comments on Corophioid amphipods for LH dcadien 30August04 II. Ischyrocerids Family Ischyroceridae rd The ischyrocerids, like the ampithoids you will meet later, all have distinctive 3 uropods. Both the inner and outer rami bear embedded terminal spines whose fine dentition is of value in specific determination. They are clearly corophioids based on their eyes, which are usually relatively large and range from obtusely triangular to rectangular to oval to round. They also show the same ontogenic changes in relative strength of gnathopods discussed above in isaeid; the male strongly, and the female weakly. They are tubicolous, and bear spinning glands and modified dactyls for the extrusion of amphipod silk. We have nine genera from this family represented in the SCB; Cerapus, Ericthonius, Ischyrocerus, Jassa, Microjassa, Neoischyrocerus, Parajassa, Ventojassa, and the deep water Bonnierella. There is not currently a key available to separate them all. I will attempt to provide one below. Key to the genera of Ischyroceridae in the NEP (modified from J. L. Barnard 1973) dcadien - 28 August 2004 1. U3 with 1 ramus, or with 2 rami of which 1 is vestigial 2 U3 with 2 fully developed rami 3 2. U2 with 1 ramus Cerapus U2 with 2 rami Ericthonius 3. Article 2 of Per 5-7 linear; article 4 of mxpd palp clawlike and larger than article 3 Bonnierella Article 2 of Per 5-7 subovate or broadly rectangular; article 4 of mxpd palp shorter than article 3 and blunt or subconical 4 4. Article 5 of G1 much longer than article 6 Ventojassa Article 5 of G1 as long as or shorter than article 6 5 5.
    [Show full text]
  • Bering Sea Marine Invasive Species Assessment Alaska Center for Conservation Science
    Bering Sea Marine Invasive Species Assessment Alaska Center for Conservation Science Scientific Name: Jassa marmorata Phylum Arthropoda Common Name a tube-building amphipod Class Malacostraca Order Amphipoda Family Ischyroceridae Z:\GAP\NPRB Marine Invasives\NPRB_DB\SppMaps\JASMAR.pn g 24 Final Rank 57.18 Data Deficiency: 11.25 Category Scores and Data Deficiencies Total Data Deficient Category Score Possible Points Distribution and Habitat: 25 26 3.75 Anthropogenic Influence: 6.75 10 0 Biological Characteristics: 16 25 5.00 Impacts: 3 28 2.50 Figure 1. Occurrence records for non-native species, and their geographic proximity to the Bering Sea. Ecoregions are based on the classification system by Spalding et al. (2007). Totals: 50.75 88.75 11.25 Occurrence record data source(s): NEMESIS and NAS databases. General Biological Information Tolerances and Thresholds Minimum Temperature (°C) -2 Minimum Salinity (ppt) 12 Maximum Temperature (°C) 27 Maximum Salinity (ppt) 38 Minimum Reproductive Temperature (°C) NA Minimum Reproductive Salinity (ppt) 31* Maximum Reproductive Temperature (°C) NA Maximum Reproductive Salinity (ppt) 35* Additional Notes J. marmot is a tube-building amphipod, greyish in color with red-brown markings. Its maximum length is 10 mm and there are two distinct morphs of males with two different mating strategies. The 'major' morphs are fighter males, while the 'minor' morphs are sneaker males. This species is difficult to identify in the field, and easily confused with other Jassa species. There is some uncertainty around its native distribution due to the difficulty of distinguishing between J. marmorata and similar species, but it is likely native to the northwest Atlantic.
    [Show full text]
  • Final Supplemental Environmental Impact Statement Maury Island Aquatic Reserve
    Final Supplemental Environmental Impact Statement Maury Island Aquatic Reserve Lead Agency: Washington State Department of Natural Resources Aquatic Resources Program October 29, 2004 Final Supplemental Environmental Impact Statement – Proposed Maury Island Aquatic Reserve Fact sheet – Project Title: Maury Island Aquatic Reserve Final Supplemental Environmental Impact Statement (SEIS) for the Department of Natural Resources Aquatic Reserves Program Project Description: The purpose of this action is to adopt and implement appropriate management strategies for state-owned aquatic lands at the Maury Island site, which includes Quartermaster Harbor and the eastern shoreline of Maury Island. Maury Island is in King County, Township 21 North, Range 02 East and 03 East, and Township 22 North, Range 02 East and 03 East. This non-project Final Supplemental Environmental Impact Statement provides an opportunity for the public and private sector, affected tribes, and agencies with jurisdiction, expertise, and interest to review and comment on the proposed action by the Washington State Department of Natural Resources (DNR) to implement appropriate management strategies for the Maury Island site. This document analyzes reasonable alternatives, the probable significant adverse and beneficial environmental impacts of the alternatives, and their relation to existing policies, rules, and regulations. The Aquatic Reserves Program Guidance Final Programmatic Environmental Impact Statement (FEIS) was issued on September 6, 2002 to define criteria for establishing an aquatic reserve. The Maury Island Aquatic Reserve SEIS implements the guidance. Copies of the programmatic FEIS are available for review through either the SEPA Center or the Aquatic Resources Division, Washington Department of Natural Resources, 1111 St. SE, Olympia, Washington, or the State Library.
    [Show full text]