Sediment Microbial Fuel Cells As a Barrier to Sulfide Accumulation And
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Microbial Carbon Metabolism Associated with Electrogenic Sulphur Oxidation in Coastal Sediments
The ISME Journal (2015) 9, 1966–1978 & 2015 International Society for Microbial Ecology All rights reserved 1751-7362/15 OPEN www.nature.com/ismej ORIGINAL ARTICLE Microbial carbon metabolism associated with electrogenic sulphur oxidation in coastal sediments Diana Vasquez-Cardenas1,2, Jack van de Vossenberg2,6, Lubos Polerecky3, Sairah Y Malkin4,7, Regina Schauer5, Silvia Hidalgo-Martinez2, Veronique Confurius1, Jack J Middelburg3, Filip JR Meysman2,4 and Henricus TS Boschker1 1Department of Marine Microbiology, Royal Netherlands Institute for Sea Research (NIOZ), Yerseke, The Netherlands; 2Department of Ecosystem Studies, Royal Netherlands Institute for Sea Research (NIOZ), Yerseke, The Netherlands; 3Department of Earth Sciences, Utrecht University, Utrecht, The Netherlands; 4Department of Environmental, Analytical and Geo-Chemistry, Vrije Universiteit Brussel (VUB), Brussels, Belgium and 5Centre of Geomicrobiology/Microbiology, Department of Bioscience, Aarhus University, Aarhus, Denmark Recently, a novel electrogenic type of sulphur oxidation was documented in marine sediments, whereby filamentous cable bacteria (Desulfobulbaceae) are mediating electron transport over cm- scale distances. These cable bacteria are capable of developing an extensive network within days, implying a highly efficient carbon acquisition strategy. Presently, the carbon metabolism of cable bacteria is unknown, and hence we adopted a multidisciplinary approach to study the carbon substrate utilization of both cable bacteria and associated microbial community in sediment incubations. Fluorescence in situ hybridization showed rapid downward growth of cable bacteria, concomitant with high rates of electrogenic sulphur oxidation, as quantified by microelectrode profiling. We studied heterotrophy and autotrophy by following 13C-propionate and -bicarbonate incorporation into bacterial fatty acids. This biomarker analysis showed that propionate uptake was limited to fatty acid signatures typical for the genus Desulfobulbus. -
Cable Bacteria Generate a Firewall Against Euxinia in Seasonally Hypoxic Basins
Cable bacteria generate a firewall against euxinia in seasonally hypoxic basins Dorina Seitaja,1, Regina Schauerb, Fatimah Sulu-Gambaric, Silvia Hidalgo-Martineza, Sairah Y. Malkind,2, Laurine D. W. Burdorfa, Caroline P. Slompc, and Filip J. R. Meysmana,d,1 aDepartment of Ecosystem Studies, Royal Netherlands Institute for Sea Research, 4401 NT Yerseke, The Netherlands; bCenter for Microbiology, Department of Bioscience, Aarhus University, 8000 Aarhus, Denmark; cDepartment of Earth Sciences–Geochemistry, Faculty of Geosciences, Utrecht University, 3584 CD Utrecht, The Netherlands; and dDepartment of Analytical, Environmental, and Geochemistry, Vrije Universiteit Brussel, 1050 Brussels, Belgium Edited by Donald E. Canfield, Institute of Biology and Nordic Center for Earth Evolution, University of Southern Denmark, Odense M, Denmark, and approved September 10, 2015 (received for review May 23, 2015) Seasonal oxygen depletion (hypoxia) in coastal bottom waters can present, the environmental controls on the timing and formation lead to the release and persistence of free sulfide (euxinia), which of coastal euxinia are poorly understood. is highly detrimental to marine life. Although coastal hypoxia is Here we document a microbial mechanism that can delay or relatively common, reports of euxinia are less frequent, which even prevent the development of euxinia in seasonally hypoxic suggests that certain environmental controls can delay the onset basins. The mechanism is based on the metabolic activity of a of euxinia. However, these controls and their prevalence are newly discovered type of electrogenic microorganism, named poorly understood. Here we present field observations from a cable bacteria (Desulfobulbaceae, Deltaproteobacteria), which seasonally hypoxic marine basin (Grevelingen, The Netherlands), which suggest that the activity of cable bacteria, a recently discov- are capable of inducing electrical currents over centimeter-scale ered group of sulfur-oxidizing microorganisms inducing long-distance distances in the sediment (12, 13). -
ENERGY MANAGEMENT of MICROBIAL FUEL CELLS for HIGH EFFICIENCY WASTEWATER TREATMENT and ELECTRICITY GENERATION by FERNANDA LEITE
ENERGY MANAGEMENT OF MICROBIAL FUEL CELLS FOR HIGH EFFICIENCY WASTEWATER TREATMENT AND ELECTRICITY GENERATION by FERNANDA LEITE LOBO B.S., Universidade do Estado do Amazonas, 2012 B.S., Universidade Federal do Amazonas, 2013 M.S., University of Colorado Boulder, 2017 A thesis submitted to the Faculty of the Graduate School of the University of Colorado in partial fulfillment of the requirement for the degree of Doctor of Philosophy Department of Civil, Environmental, and Architectural Engineering 2018 This thesis entitled: Energy Management of Microbial Fuel Cells for High Efficiency Wastewater Treatment and Electricity Generation written by Fernanda Leite Lobo has been approved for the Department of Civil, Environmental, and Architectural Engineering Jae-Do Park, Ph.D. JoAnn Silverstein, Ph.D. Mark Hernandez, Ph.D. Rita Klees, Ph.D. Zhiyong (Jason) Ren, Ph.D. Date The final copy of this thesis has been examined by the signatories, and we find that both the content and the form meet acceptable presentation standards of scholarly work in the above mentioned discipline. ii Leite Lobo, Fernanda (Ph.D., Environmental Engineering) Energy Management of Microbial Fuel Cells for High Efficiency Wastewater Treatment and Electricity Generation Thesis directed by Associate Professor Zhiyong Jason Ren Abstract In order to develop communities in a sustainable manner it is necessary to think about how to provide basic and affordable services including sanitation and electricity. Wastewater has energy embedded in the form biodegradable organic matter, but most of the conventional systems use external energy to treat the wastewater instead of harvest its energy. Microbial fuel cells (MFCs) are unique systems that are capable of converting chemical energy of biodegradable substrates embedded in the waste materials into renewable electricity. -
Microbial Structure and Energy Generation in Microbial Fuel Cells Powered with Waste Anaerobic Digestate
energies Article Microbial Structure and Energy Generation in Microbial Fuel Cells Powered with Waste Anaerobic Digestate Dawid Nosek * and Agnieszka Cydzik-Kwiatkowska Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Słoneczna 45 G, 10-709 Olsztyn, Poland; [email protected] * Correspondence: [email protected]; Tel.: +48-89-523-4144; Fax: +48-89-523-4131 Received: 19 July 2020; Accepted: 7 September 2020; Published: 10 September 2020 Abstract: Development of economical and environment-friendly Microbial Fuel Cells (MFCs) technology should be associated with waste management. However, current knowledge regarding microbiological bases of electricity production from complex waste substrates is insufficient. In the following study, microbial composition and electricity generation were investigated in MFCs powered with waste volatile fatty acids (VFAs) from anaerobic digestion of primary sludge. Two anode sizes were tested, resulting in organic loading rates (OLRs) of 69.12 and 36.21 mg chemical oxygen demand (COD)/(g MLSS d) in MFC1 and MFC2, respectively. Time of MFC operation affected the microbial · structure and the use of waste VFAs promoted microbial diversity. High abundance of Deftia sp. and Methanobacterium sp. characterized start-up period in MFCs. During stable operation, higher OLR in MFC1 favored growth of exoelectrogens from Rhodopseudomonas sp. (13.2%) resulting in a higher and more stable electricity production in comparison with MFC2. At a lower OLR in MFC2, the percentage of exoelectrogens in biomass decreased, while the abundance of genera Leucobacter, Frigoribacterium and Phenylobacterium increased. In turn, this efficiently decomposed complex organic substances, favoring high and stable COD removal (over 85%). -
Enrichment of Electrochemically Active Bacteria Using Microbial Fuel Cell and Potentiostat
Enrichment of electrochemically active bacteria using microbial fuel cell and potentiostat Tim Niklas Enke ETH Zurich [email protected] – [email protected] Microbial Diversity 2015 Introduction Microbial fuel cells (MFC) can be applied to harness the power released by metabolically active bacteria as electrical energy (Figure 1). In addition to the energy generation capabilities of MFC, they have been used to generate hydrogen gas and to clean, desalinate or detoxify wastewater [1,2]. Among the bacteria found to be electrochemically active are Geobacter sulfurreducens, Shewanella putrefaciens and Aeromonas hydrophila [2,3,4]. Figure 1: Scheme of a microbial fuel cell. A MFC consists of an anaerobic anode chamber with rich organic matter, such as sludge from wastewater treatment plants or sediment. The anode (1) serves as an electron acceptor in an electron acceptor limited environment and is wired externally (2) over a resistor (3) to a cathode (5). Electrons travel over the circuit and create a current, while protons can pass the proton exchange membrane (4) to reach the oxic cathode chamber. At the cathode, the protons, electrons and oxygen react to form water. In the cathode chamber, a catalyst can facilitate the reaction and thus the movement of electrons. Figure from [https://illumin.usc.edu/assets/media/175/MFCfig2p1.jpg , 08/18/2015]. Even more remarkably, in the deep sea, microbes can power measurement devices that deploy an anode in the anoxic sediments and position a cathode in the oxygen richer water column above, thus exploiting the MFC principle [5]. In a different application, MFC can be used to enrich for bacteria that are capable of extracellular electron transfer (EET) and form a biofilm on the electrode. -
Mineral Formation Induced by Cable Bacteria Performing Long-Distance Electron Transport in Marine Sediments
Biogeosciences, 16, 811–829, 2019 https://doi.org/10.5194/bg-16-811-2019 © Author(s) 2019. This work is distributed under the Creative Commons Attribution 4.0 License. Mineral formation induced by cable bacteria performing long-distance electron transport in marine sediments Nicole M. J. Geerlings1, Eva-Maria Zetsche2,3, Silvia Hidalgo-Martinez4, Jack J. Middelburg1, and Filip J. R. Meysman4,5 1Department of Earth Sciences, Utrecht University, Princetonplein 8a, 3584 CB Utrecht, the Netherlands 2Department of Marine Sciences, University of Gothenburg, Carl Skottsberg gata 22B, 41319 Gothenburg, Sweden 3Department of Estuarine and Delta Systems, Royal Netherlands Institute for Sea Research, Utrecht University, Korringaweg 7, 4401 NT Yerseke, the Netherlands 4Department of Biology, Ecosystem Management Research Group, Universiteit Antwerpen, Universiteitsplein 1, 2160 Antwerp, Belgium 5Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, the Netherlands Correspondence: Nicole M. J. Geerlings ([email protected]) Received: 10 October 2018 – Discussion started: 22 October 2018 Revised: 10 January 2019 – Accepted: 26 January 2019 – Published: 13 February 2019 Abstract. Cable bacteria are multicellular, filamentous mi- esize that the complete encrustation of filaments might create croorganisms that are capable of transporting electrons over a diffusion barrier and negatively impact the metabolism of centimeter-scale distances. Although recently discovered, the cable bacteria. these bacteria appear to be widely present in the seafloor, and when active they exert a strong imprint on the local geochemistry. In particular, their electrogenic metabolism in- 1 Introduction duces unusually strong pH excursions in aquatic sediments, which induces considerable mineral dissolution, and subse- 1.1 Cable bacteria quent mineral reprecipitation. -
Enhanced Performance of Microbial Fuel Cells by Using Mno2/Halloysite Nanotubes to Modify Carbon Cloth Anodes
Enhanced performance of microbial fuel cells by using MnO2/Halloysite nanotubes to modify carbon cloth anodes Item Type Article Authors Chen, Yingwen; Chen, Liuliu; Li, Peiwen; Xu, Yuan; Fan, Mengjie; Zhu, Shemin; Shen, Shubao Citation Enhanced performance of microbial fuel cells by using MnO2/ Halloysite nanotubes to modify carbon cloth anodes 2016, 109:620 Energy DOI 10.1016/j.energy.2016.05.041 Publisher PERGAMON-ELSEVIER SCIENCE LTD Journal Energy Rights © 2016 Elsevier Ltd. All rights reserved. Download date 29/09/2021 22:45:04 Item License http://rightsstatements.org/vocab/InC/1.0/ Version Final accepted manuscript Link to Item http://hdl.handle.net/10150/621214 Enhanced Performance of Microbial Fuel Cells by Using MnO2/Halloysite Nanotubes to modify carbon cloth anodes Yingwen Chen1,*,†, Liuliu Chen1,†, Peiwen Li2, Yuan Xu1, Mengjie Fan1, Shemin Zhu3, 1 Shubao Shen 1College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210009, China 2Department of Aerospace and Mechanical Engineering, the University of Arizona, Tucson, AZ 85721, USA 3College of Material Science and Engineering, Nanjing Tech University, Nanjing 210009, China † The authors contributed equally to this work. *Corresponding author at: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No.30 Puzhu south Road, Nanjing, 210009, PR China TEL: (+86 25) 58139922 FAX: (+86 25) 58139922 E-mail address: [email protected] (Yingwen Chen) ABSTRACT: The modification of anode materials is important to enhance the power generation of microbial fuel cells (MFCs). A novel and cost-effective modified anode that is fabricated by dispersing manganese dioxide (MnO2) and Halloysite nanotubes (HNTs) on carbon cloth to improve the MFCs’ power production was reported. -
Microfluidic Microbial Fuel Cells for Microstructure Interrogations By
Microfluidic Microbial Fuel Cells for Microstructure Interrogations by Erika Andrea Parra A dissertation submitted in partial satisfaction of the requirements for the degree of Doctor of Philosophy in Engineering - Mechanical Engineering in the Graduate Division of the University of California, Berkeley Committee in charge: Professor Liwei Lin, Chair Professor Carlos Fendandez-Pello Professor John D. Coates Fall 2010 Microfluidic Microbial Fuel Cells for Microstructure Interrogations Copyright 2010 by Erika Andrea Parra 1 Abstract Microfluidic Microbial Fuel Cells for Microstructure Interrogations by Erika Andrea Parra Doctor of Philosophy in Engineering - Mechanical Engineering University of California, Berkeley Professor Liwei Lin, Chair The breakdown of organic substances to retrieve energy is a naturally occurring process in nature. Catabolic microorganisms contain enzymes capable of accelerating the disintegration of simple sugars and alcohols to produce separated charge in the form of electrons and protons as byproducts that can be harvested extracellularly through an electrochemical cell to produce electrical energy directly. Bioelectrochemical energy is then an appealing green alternative to other power sources. However, a number of fundamental questions must be addressed if the technology is to become economically feasible. Power densities are low, hence the electron flow through the system: bacteria-electrode connectivity, the volumetric limit of catalyst loading, and the rate-limiting step in the system must be understood and optimized. This project investigated the miniaturization of microbial fuel cells to explore the scaling of the biocatalysis and generate a platform to study fundamental microstructure effects. Ultra- micro-electrodes for single cell studies were developed within a microfluidic configuration to quantify these issues and provide insight on the output capacity of microbial fuel cells as well as commercial feasibility as power sources for electronic devices. -
Computational Simulation of Mass Transport and Energy Transfer in the Microbial Fuel Cell System
University of Tennessee, Knoxville TRACE: Tennessee Research and Creative Exchange Doctoral Dissertations Graduate School 12-2015 Computational Simulation of Mass Transport and Energy Transfer in the Microbial Fuel Cell System Shiqi Ou University of Tennessee - Knoxville, [email protected] Follow this and additional works at: https://trace.tennessee.edu/utk_graddiss Part of the Catalysis and Reaction Engineering Commons, Energy Systems Commons, Numerical Analysis and Computation Commons, and the Transport Phenomena Commons Recommended Citation Ou, Shiqi, "Computational Simulation of Mass Transport and Energy Transfer in the Microbial Fuel Cell System. " PhD diss., University of Tennessee, 2015. https://trace.tennessee.edu/utk_graddiss/3598 This Dissertation is brought to you for free and open access by the Graduate School at TRACE: Tennessee Research and Creative Exchange. It has been accepted for inclusion in Doctoral Dissertations by an authorized administrator of TRACE: Tennessee Research and Creative Exchange. For more information, please contact [email protected]. To the Graduate Council: I am submitting herewith a dissertation written by Shiqi Ou entitled "Computational Simulation of Mass Transport and Energy Transfer in the Microbial Fuel Cell System." I have examined the final electronic copy of this dissertation for form and content and recommend that it be accepted in partial fulfillment of the equirr ements for the degree of Doctor of Philosophy, with a major in Mechanical Engineering. Matthew M. Mench, Major Professor We have read this dissertation and recommend its acceptance: Kivanc Ekici, Feng-Yuan Zhang, Vasilios Alexiades Accepted for the Council: Carolyn R. Hodges Vice Provost and Dean of the Graduate School (Original signatures are on file with official studentecor r ds.) Computational Simulation of Mass Transport and Energy Transfer in the Microbial Fuel Cell System A Dissertation Presented for the Doctor of Philosophy Degree The University of Tennessee, Knoxville Shiqi Ou December 2015 ii Copyright © 2015 by Shiqi Ou All rights reserved. -
Optimization of the Electrolyte Parameters and Components in Zinc Particle Fuel Cells
energies Article Optimization of the Electrolyte Parameters and Components in Zinc Particle Fuel Cells Thangavel Sangeetha 1,2,† , Po-Tuan Chen 3,† , Wu-Fu Cheng 4, Wei-Mon Yan 1,2,* and K. David Huang 4,* 1 Department of Energy and Refrigerating Air-Conditioning Engineering, National Taipei University of Technology, Taipei 10608, Taiwan; [email protected] 2 Research Center of Energy Conservation for New Generation of Residential, Commercial, and Industrial Sectors, National Taipei University of Technology, Taipei 10608, Taiwan 3 Center for Condensed Matter Sciences, National Taiwan University, Taipei 10617, Taiwan; [email protected] 4 Department of Vehicle Engineering, National Taipei University of Technology, Taipei 10608, Taiwan; [email protected] * Correspondence: [email protected] (W.-M.Y.); [email protected] (K.D.H.); Tel.: +886-2-2771-2171 (ext. 3676) (K.D.H.) † These authors contributed equally to this work (TS and PTC). Received: 18 February 2019; Accepted: 19 March 2019; Published: 21 March 2019 Abstract: Zinc (Zn)-air fuel cells (ZAFC) are a widely-acknowledged type of metal air fuel cells, but optimization of several operational parameters and components will facilitate enhanced power performance. This research study has been focused on the investigation of ZAFC Zn particle fuel with flowing potassium hydroxide (KOH) electrolyte. Parameters like optimum electrolyte concentration, temperature, and flow velocity were optimized. Moreover, ZAFC components like anode current collector and cathode conductor material were varied and the appropriate materials were designated. Power performance was analyzed in terms of open circuit voltage (OCV), power, and current density production and were used to justify the results of the study. -
Microbial Fuel Cells for Improved Bioremediation
Microbial Fuel Cells for Improved Bioremediation Emi Lemberg1, Joe Vallino2 1University of Chicago 5801 S Ellis Ave, Chicago IL 60637 USA 2The Ecosystems Center: Semester in Environmental Science Marine Biological Laboratory Woods Hole, Massachusetts 02543 USA 2017 Fall Semester 1 Abstract Microbial fuel cells (MFCs) can be used as a power source and as a tool for bioremediation. By creating an electrical connection between the anaerobic sediments and aerobic water column, a MFC can increase the metabolism rates of bacteria in the sediment, allowing the bacteria to break down complex molecules they would not be able to consume otherwise. This experiment focuses on the breakdown of organic matter in sediments from Little Pond in Falmouth, Massachusetts. While the sediments already contain high concentrations of organic matter, caffeine was amended to the sediments to as a proxy for difficult to degrade pollutants. This experiment examines the effect of an added 2 V potential has on of organic matter decomposition in a sediment MFC (Driven MFC). During the incubation, a pH gradient developed in the Driven MFC, with a change in pH of 1.12 on the final day of incubation. The pH gradient caused the current of the MFCs to drop from approximately 2.5 mA to approximately 0.05 mA halfway through the experiment. Additionally, the high pH at the surface of the MFC resulted in the uptake of carbon dioxide for the first 17 days of the incubation. Organic carbon extracted from the sediments suggests that a decrease in the carbon content of sediments occurred; however, the short-term nature of the incubations prevented a statistically significant conclusion when compared to the controls. -
Effects of Meiofauna and Cable Bacteria on Oxygen, Ph and Sulphide Dynamics in Baltic Sea Hypoxic Sediment
Effects of meiofauna and cable bacteria on oxygen, pH and sulphide dynamics in Baltic Sea hypoxic sediment A sediment core experiment with focus on different abundances of meiofauna and meiofaunal bioturbation in hypoxic Baltic Sea sediments. Meiofaunal effect on redox biogeochemistry of the sediment was studied in co-existence with cable bacteria. Johanna Hedberg Department of Ecology, Environment and Plant sciences M.Sc. Thesis 60 ECTS credits Marine biology Master´s Programme in Marine Biology (120 ECTS credits) Spring term 2019 Supervisor: Stefano Bonaglia Effects of meiofauna and cable bacteria on oxygen, pH and sulphide dynamics in Baltic Sea hypoxic sediment A sediment core experiment with focus on different abundances of meiofauna and meiofaunal bioturbation in hypoxic Baltic Sea sediments. Meiofaunal effect on redox biogeochemistry of the sediment was studied in co-existence with cable bacteria. Johanna Hedberg Abstract Oxygen depleted areas in the Baltic Sea are wide spread and affecting sediment biogeochemistry leading to faunal migration by formation of toxic sulphide. In sediments where reoxygenation events occur, re- colonization of meiofauna and cable bacteria are believed to enhance ideal conditions and facilitate recolonization of other fauna. In sediments world-wide most abundant are bioturbating meiofauna representing various phyla grouped by body size (>0.04 and <1 mm) and known to enhance bacterial activity. Meiofaunal abundance and meiofaunal bioturbation in sulfidic sediments and effects on bacterial community structure is currently poorly understood. As second thesis to find filament building sulphide oxidising cable bacteria in the Baltic Proper, their co-existence with meiofauna and effect on sediment were also studied. Alive meiofauna was added to otherwise intact cores creating gradients of abundance (CTR = unmanipulated cores, DEBRIS = debris addition, LM = low meiofauna abundance and HM = high meiofauna abundance, n = 3) and microsensor profiles of oxygen, pH and sulphide were measured weekly for three weeks to obtain effects.