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Integrin-Alpha 4 / Cd49d (CT) Antibody

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Integrin-Alpha 4 / Cd49d (CT) Antibody 21.10.2014Integrin-alpha 4 / CD49d (CT) antibody Rabbit Anti-Human/Mouse/Rat Integrin-alpha 4 (CD49d, ITGA4, IA4; CT) Instruction Manual Catalog Number PK-AB718-4783 Synonyms Integrin alpha 4 Antibody: CD49d, ITGA4, IA4, alpha 4 subunit of VLA-4 receptor Description The Integrin-alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha 4 chain associates with either beta 1 or beta 7 chain. It has been demonstrated that the putative ligand-binding sites of both Integrin-alpha 4 beta 1 and alpha 4 beta 7 is located on the alpha 4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha 4 beta 7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though Integrin-alpha 4 beta 7, the gut mucosal homing receptor for peripheral T cells. Despite its predicted molecular weight, Integrin alpha 4 will often run at higher molecular weight in SDS-PAGE. At least two isoforms are known to exist. The immunogen to this antibody is outside of the Integrin-alpha 4 recombinant protein fragment and thus will not recognize it. Quantity 100 µg Source / Host Rabbit Immunogen Integrin-alpha 4 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human Integrin-alpha 4. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human, Mouse, Rat Specificity At least two isoforms are known to exist. The immunogen to this antibody is outside of the Integrin alpha4 recombinant protein fragment and thus will not recognize it. Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 1 µg/mL. Despite its predicted molecular weight, Integrin alpha4 will often run at higher molecular weight in SDS-PAGE. Images Available upon request. References Wegener KL and Campbell ID. Transmembrane and cytoplasmic domains in integrin activation and protein-protein interactions. Mol. Membr. Biol. 2008; 25:376-87. Kamata T, Puzon W, and Takada Y. Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29). Biochem J. 1995; 305:945-51. Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 1993; 74:185-95. Pulido R, Elices MJ, Campanero MR, et al. Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes. J. Biol. Chem. 1991; 266:10241-5. Images Available upon request. Related Products Cat.No. PK-AB718-4783P - Integrin alpha 4 Peptide Cat. No. PK-AB718-1205 - Jurkat Cell Lysate FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES. PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014 .
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