Fundamentals of Microsurgery

David A. Wilkie, DVM, MS, Diplomate ACVO Professor Department Chair The Ohio State University [email protected] Microsurgery

 Ophthalmic  Vascular  Urogenital  Neurologic Microsurgery

 Definition  utilizing magnification and small, handheld instruments and suture to correct defects in small &/or delicate tissues 16th CENTURY

Ouch! To do a job….you need the right tools Microsurgery  Differs from traditional surgery in:  Surgeon position  Magnification  Specialized instrumentation  Suture and needle size

Dr. Dyce Surgeon Position

 Surgical Position  Seated  Specialized chairs with armrests  resting on armrest  Essential for fine motor control Microsurgery

 Surgical Position  Seated  Specialized chairs with armrests  Arms resting on table or armrest  Essential for fine motor control  Able to adjust height Height is adjustable Elbows and wrists are locked Magnification - There are choices… Fundamentals of Microsurgery

 Magnification – purpose:  provide an improved view of the tissues of concern  Will vary by tissue of interest  allow a comfortable working distance for the surgeon  Back straight, arms at 90 degrees  facilitate adjustment of the interpupillary distance to suit the surgeon  permit a wide field of view Microsurgery

 Differs from traditional surgery in:  Specialized instrumentation  Surgeon position  Magnification  Type & size of suture Human Hair

9-0 MonofilamentVicryl Fundamentals of Microsurgery

 Rules of microsurgery are meant as a foundation to guide the beginning ophthalmic surgeon  Once understood, rules can occasionally be molded and adapted to suit the surgeon and the individual patient  The surgeon must however always re-visit the basic microsurgical rules and principles when a new or unfamiliar technique or procedure is to be performed Fundamentals of Microsurgery

 Surgeons must have a goal and a plan to achieve the goal, but must also be adaptable and familiar with more than one technique so that obstacles encountered during the surgical procedure may be overcome. Fundamentals of Microsurgery

 Not all proceed according to the plan.  All eyes are different and the surgeon who says “I always do it this way” is destined to encounter situations where their technique does not meet the patients needs. Fundamentals of Microsurgery

 Think of surgery like your commute to work  We may all travel a different a route and method  We get there in various ways, BUT…..we all get to the same destination Fundamentals of Microsurgery

 Think of surgery like your drive to work  You have a route that you usually take  Do you know other routes?  What if there is an accident or detour, how would you respond? Fundamentals of Microsurgery

 Regardless of our individual variations, we must all follow the basic rules:  to use appropriate magnification and instrumentation  to be efficient and precise  to ensure minimal tissue trauma  to minimize surgical time  to maintain the anterior chamber using small incisions and viscoelastic materials  to obtain excellent tissue wound apposition with the smallest and most appropriate suture materials  to achieve a successful, comfortable, cosmetic and whenever possible, visual outcome Fundamentals of Microsurgery

 The surgeon must at all times keep several ophthalmic microsurgical principles and rules in mind:  time is trauma  tissues should be handled as little and as efficiently as possible  ophthalmic microsurgery procedures are comprised of a series of steps in sequence, each one designed to move the surgery forward towards the goal. The surgeon must be familiar with these steps, perform them economically, efficiently and atraumatically, making no wasted movements and moving steadily forward towards the surgical goal. Fundamentals of Microsurgery

 Magnification  Began in 1876 using simple head loupes  First microscope for human ophthalmic surgery described in 1950 and in veterinary in the 1970’s Fundamentals of Microsurgery

 Magnification – purpose:  provide an improved view of the tissues of concern  Will vary by tissue of interest  allow a comfortable working distance for the surgeon  Back straight, arms at 90 degrees  facilitate adjustment of the interpupillary distance to suit the surgeon  permit a wide field of view Fundamentals of Microsurgery

 Magnification  Beginning surgeons should start with magnification early as part of their basic training  Will improve their tissue handling and appreciation for tissue trauma and wound apposition Microsurgery

 Magnification  Eyelids, conjunctiva  2-5X  Cornea, urogenital  5-10X  Intraocular, vascular, neurologic  8-15X 2.5X 10X

5X 15X Fundamentals of Microsurgery

 Magnification  Essential to select the appropriate magnification.  If we consider ourselves ophthalmic microsurgeons then we must use the appropriate magnification at all times. Fundamentals of Microsurgery

 Magnification  Head Loupes  used for microsurgery when operating microscopes are not available such as in the field or a large animal barn setting and for orbital and eyelid surgeries  Operating Microscope  Better choice, especially for ≥ 5X Fundamentals of Microsurgery

 Magnification  Head Loupes  Simple loupes  Galilean-type loupes  Prismatic loupes (Keplerian) Fundamentals of Microsurgery

 Magnification  Simple Loupes  one pair of positive meniscus lenses  limited by spherical aberration and color fringing  plastic construction  fixed interpupillary distance  very short working distance  poor surgeon body and position  strain on the surgeon’s neck and back.  POOR CHOICE Fundamentals of Microsurgery

 Magnification  Galilean Loupes  up to 2.5x magnification  multiple lenses to offer magnification and are generally lightweight and less expensive  adjustable interpupillary distance  working distance varies Fundamentals of Microsurgery

 Magnification  Prismatic Loupes  up to 8.0x magnification  ≥5.0x a microscope is preferred  highest optical quality  series of lenses and prisms to magnify the subject  similar in principle to low-power telescopes  greater magnification  sharp resolution  greater depth of field  heavier and more expensive Fundamentals of Microsurgery

 Magnification  Prismatic Loupes  As magnification increases  they become long and heavy  Shallow depth of field  Head movements make use difficult (>5X) Fundamentals of Microsurgery

 Magnification  Loupes  Surgeons should have their own loupes  Surgeons should have both a Galilean and a Prismatic set with lower and higher magnification  Can add light source if desired Fundamentals of Microsurgery

 Magnification  Loupes - styles Fundamentals of Microsurgery

 Magnification  Loupes - light Fundamentals of Microsurgery

 Magnification  Operating Microscope  magnification from 5-40x  For ophthalmic microsurgery, magnification of 5-20x is generally sufficient.  Increased cost and maintenance  longer surgical setup time  less intraoperative positioning flexibility  less portability Fundamentals of Microsurgery

 Magnification  Operating Microscope  Coaxial illumination  Projected through viewing objective  variable magnification  motorized continuous zoom  motorized focus  motorized X-Y axis  Foot controls adjust  light, magnification, zoom, focus and X-Y axis  Allow multiple surgeons  Floor, table or ceiling mount  Additional attachments Fundamentals of Microsurgery

 Magnification  Operating Microscope  Floor mounted  Ceiling Mounted  Table Mounted  Portable  2.0-9.0X Fundamentals of Microsurgery

 Magnification  Operating Microscope  Attachments  Video/camera  laser – need a filter Fundamentals of Microsurgery

 Magnification  Operating Microscope  While portable - movement over distance is discouraged  Articulating arms with tension adjustment  Gross focus done manually prior to start of surgery Fundamentals of Microsurgery

 Magnification  Operating Microscope  Optical head has  Primary surgeon  Beam splitter  Assistant surgeon  Video attachment Fundamentals of Microsurgery

 Magnification  Operating Microscope  Objective lens 150-400mm  175 mm most common in ophthalmology  Magnification:

Magnification = Focal length of binocular tubes x magnifying power of Focal length of the objective the eye pieces x magnifying power of magnification changer Fundamentals of Microsurgery

 Magnification  Prior to surgery  Center the X-Y axis  Lowest magnification  Zero the fine focus  Adjust the gross focus manually Fundamentals of Microsurgery

 Magnification  Prior to surgery  Adjust the interpupillary distance Fundamentals of Microsurgery

 Magnification  Prior to surgery  Adjust the chair and table height Fundamentals of Microsurgery

 Magnification  Prior to surgery  Ensure the surgeons view and the video view are both in focus  Verify at the highest magnification to be used Fundamentals of Microsurgery

 Magnification  Prior to surgery  Position all foot pedals where they can be reached  With multiple foot pedals the microscope pedal goes to the non- dominant foot Fundamentals of Microsurgery

 Magnification  Prior to surgery  KNOW WHERE ALL THE FOOT PEDAL CONTROLS ARE BEFORE STARTING SURGERY Fundamentals of Microsurgery

 Magnification  Use your magnification  Adjust during surgery – cornea vs lens Fundamentals of Microsurgery

 Magnification  As magnification increases  The field of view and depth of field decrease  At 3.5X - field of view is 50mm and depth of field is 2.6mm  At 20X - field of view is 10mm and depth of field is 0.4mm Fundamentals of Microsurgery

 Magnification  As magnification increases  The field of view and depth of field decrease  At 3.5X - field of view is 50mm and depth of field is 2.6mm  At 20X - field of view is 10mm and depth of field is 0.4mm 3.5X 20X Fundamentals of Microsurgery

 Prior to surgery  Position the patient  Lateral vs dorsal recumbency  Sand bags, vacuum pillows  Eye looks up into microscope Fundamentals of Microsurgery

 Prior to surgery  Position the patient  Anesthesiologist at opposite end of the patient  To avoid tube obstruction use a 90 degree connector or a guarded endotracheal tube Fundamentals of Microsurgery

 Prior to surgery  When seated, adjust gross focus by hand, not foot pedal Microsurgery

 Surgical Position  Seated  Specialized chairs with armrests  Arms resting on table or armrest  Essential for fine motor control

Fundamentals of Microsurgery

 At the start of surgery  Turn on microscope lights  Turn off room lights Fundamentals of Microsurgery Fundamentals of Microsurgery

 At the start of surgery  Surgeon is seated comfortably  Feet reach the pedals  Back is straight  Arms at 90 degrees  Lean slightly forward  Arms on armrests  Hands positioned and supported Fundamentals of Microsurgery

 Hands must be supported  Rest on the ball of the hand or extend 5th for support Fundamentals of Microsurgery Adjust chair height  Pre-op Check Adjust chair armrest position List Adjust table height Adjust microscope height Set microscope fine focus to neutral Center X-Y axis Adjust microscope tilt Adjust interpupillary distance Set microscope to highest magnification to be used Adjust focus of oculars Ensure video and assistant images are also in focus Return microscope to low magnification Place foot pedals to be comfortably accessible Fundamentals of Microsurgery

 Pre and peri-operative medications  Antibiotics  Antiinflammatories  Mydriatics  Surgeon preference on when to initiate Fundamentals of Microsurgery

 Prior to surgery  Administer intravenous anti- inflammatory and antimicrobial drugs  Despite our best efforts we will create tissue trauma and contaminate the field

Taylor MM, Kern TJ, Riis RC, McDonough PL, Erb HN: Intraocular bacterial contamination during canine cataract surgery. J Am Vet Med Assoc. 1995; 206(11): 1716-1720.

Pre-Operative Prep

 Clip hair  0.5% povidone iodine (1:20 to 1:50 dilution)  Final prep - povidone iodine 10%  Paint the adnexa  Avoid chlorhexidine  Protective ointment for non-surgical eye

Fundamentals of Microsurgery

 Anesthesia  General anesthesia is required for microsurgery  Non-depolarizing neuromuscular blocking agents may be required Microsurgery

 Patient position critical  No movement under anesthesia  Sand bags  Vacuum pillows  Paralysis

Patient Position paralysis Atracurium Neuromuscular blockade

 Most common is Atracurium  0.2 mg/kg dog and cat; 0.02-0.06 mg/kg horse  If needed a 2nd injection 0.1 mg/kg dog and cat; 0.025mg/kg horse  “low dose” – still ventilate to avoid respiratory acidosis  provides excellent globe exposure and will minimize globe compression as a result of extraocular muscle tension  Reversal - edrophonium 0.5 mg/kg or neostigmine 0.02mg/kg  anticholinergic (glycopyrrolate 0.02mg/kg IV or atropine 0.04mg/kg IV in small animals; glycopyrrolate 0.005 mg/kg IV in horses) should be administered concurrently with neostigmine

Fundamentals of Microsurgery

 Equine considerations Induction Patient Position unilateral vs bilateral Equine Microsurgery

 Patient position critical  No movement under anesthesia  Sand bags  Inner tube  Vacuum pillows  Paralysis  Retrobulbar Block

Patient Position

Courtesy – Dr. Brian Gilger Patient Position

Unilateral lateral recumbency Bilateral dorsal recumbency Equipment Position

Opposite to the surgeon phaco, microscope Difficult to get comfortable foot position awkward Do not expect to be comfortable Equine Recovery Fundamentals of Microsurgery  Patient  Drapes  Water repellant  Adhesive Fundamentals of Microsurgery  Patient  Speculum  Avoid external pressure on the globe  Globe collapse  Iris prolapse  Vitreous prolapse  Extrusion of visco Fundamentals of Microsurgery

 Patient  Epinephrine 1:10,000 for  Vasoconstriction  Hemostasis  Decrease fibrin  Mydriasis

Preservative vs preservative-free Tools to manage small pupil and lens stability issues

 Epinephrine  Viscoelastic  Lidocaine

Miosis

 Lidocaine  Intracameral  Preservative-free  1-2%  0.1 – 0.3ml  Onset: 1-10 minutes  Duration: 72-142 minutes  No systemic effects Miosis  Intracameral lidocaine  Dose-dependant onset & duration

Park SA, et al: The mydriatic effect of intracameral lidocaine in clinically normal dogs. Am J Vet Res 70:1521-5, 2009 Personally - I feel this helps with ECP patients Small Pupil – Epi, Lido, Visco

Jack Russell 5yr Lens instability IOP controlled with latanoprost

<15kg 1ml lidocaine + 1ml bupivacaine

>15kg 1.5ml lidocaine + 1.5ml bupivacaine Miochol® post phaco/IOL

Corneal/Lens laceration – corneal repair, phaco, suture IOL Perfect Pupil® Perfect Pupil® Malyugin Ring MST Technologies Malyugin Ring - cadaver Small Pupil – Sphincterotomy Fundamentals of Microsurgery

 Patient  Stay suture  Include the episclera Fundamentals of Microsurgery

 Patient  Lateral canthotomy?  More common for beginning surgeons  Terriers, ICLE Fundamentals of Microsurgery

 Patient  Cornea irrigated to keep moist  Assistant surgeon  Every 20-30 seconds Fundamentals of Microsurgery

 Patient  Recovery  Calm  Operated eye up – lateral or sternal recumbency  +/-Temporary Tarsorraphy, cold compress  Elizabethan collar  +/-SPL in horses Fundamentals of Microsurgery

 Instrumentation  Microsurgical instruments are typically 1/3 smaller than traditional instruments – 100mm vs 150mm  Jaws and teeth made delicate  Finish does not reflect light  Should have several packs  Basic/Minor – eyelids, orbit, adnexa  Microsurgical – cornea, intraocular  Phaco pack  Posterior segment pack  ECP pack Fundamentals of Microsurgery

 Instrumentation  Instrument choices  length of the instrument  size of the teeth and jaws  angle of the jaws  length of the jaws  sharp vs. blunt tips  straight vs. curved  flat vs. rounded handles  serrated, six sided or knurled handle grips  locking vs. non-locking  with or without a tying platform  with or without a pin stop  dull vs. polished finish  stainless steel vs. titanium Fundamentals of Microsurgery

 Instrumentation  Instrument choices  Instruments to be rotated have rounded handle  Instruments not rotated have flat handle  To prevent slippage during manipulation instrument handles are serrated, knurled or six-sided  Hinged using an X or box hinge design and spring handles as seen with Wescott tenotomy scissors or Barraquer needle holders  Pin stop to prevent over closure  Bar hinge design is used for most microsurgical forceps Fundamentals of Microsurgery

 Instrumentation  Finger pressure goes here

Pin Stop Fundamentals of Microsurgery

 Instrumentation  Bar hinge Fundamentals of Microsurgery

 Instrumentation  X or box hinge spring handles Fundamentals of Microsurgery

 Instrumentation Fundamentals of Microsurgery

 Instrumentation  Round and knurled handle Fundamentals of Microsurgery

 Instrumentation  Flat handle Fundamentals of Microsurgery  Instrumentation  Steel vs Titanium  Titanium stronger, more corrosion resistant, sharper longer  Germany vs Pakistan vs China  Quality of steel varies Fundamentals of Microsurgery

 Instrumentation X  Instrument handling  Finger movement  Pencil-like grip  Hands stabilized Microsurgery

 Hold instruments in a pencil-like grip  Finger movements

X Microsurgery

 Hold instruments like a pencil  Finger movements  Wrists and elbows fixed Microsurgery

 Tissue handling  Make no wasted movement  Handle tissues as little as possible

 IF YOU TOUCH IT….DO SOMETHING, DO NOT RELEASE UNTIL YOU HAVE DONE ALL THAT YOU CAN! Fundamentals of Microsurgery

 Instrument Handling  Delicate, precise movements  Finger movements only  Pencil grip  Arms on armrests  Elbows and wrists locked Fundamentals of Microsurgery

 Instrumentation  Instrument handling  “microsurgical instruments held in a pencil-like grip are supported by resting against the first metacarpophalangeal joint of the first finger with the fingertips of the and first finger used to control and rotate the instrument” Fundamentals of Microsurgery

 Instrumentation  Instrument handling  “Hand stability is provided by resting the outside of the 5th finger on the vacuum pillow or patients periorbital tissues” Fundamentals of Microsurgery

 Instrumentation  Instrument handling  Resist the tendency to grasp the instruments tightly as this will decrease flexibility, fatigue the hand and forearm, traumatize tissues and damage instruments and needles Fundamentals of Microsurgery Fundamentals of Microsurgery

 Instrumentation  Instrument handling  Palm or tripod grip acceptable for Steven’s tenotomy scissors Fundamentals of Microsurgery

 Instrumentation  Basic pack Basic Pack

Eyelid speculum – Barraquer wire in several sizes Forceps- Brown-Adson Colibri utility 0.3mm and 0.5mm Bishop-Harmon fine teeth Castroviejo scleral fixation Tying forceps Scissors- Stevens tenotomy – blunt, curved Westcott tenotomy – blunt, curved Needle holders - Barraquer type medium and heavy curved, non-locking, rounded and knurled handle -Derf or Alabama-Green Desmarres chalazion clamp Jaeger eyelid plate Calipers- Jameson, Castroviejo Carter sphere introducer Muscle hooks Irrigating cannulas – 21, 23, 27, 30 gauge Beaver and Bard Parker blade handles Mosquito hemostats Martinez corneal dissector Serrefine clamps Cyclodialysis spatula 1 2

3 5 4 9 7 6

10 8

11 13

12 Corneal/Intraocular Pack

Eyelid speculum – Barraquer wire in several sizes Forceps- Colibri utility .12mm and 0.3mm Bishop-Harmon delicate teeth Castroviejo scleral fixation Utrata capsulorhexis IOL folding McPherson tying – straight and angled Scissors- Stevens tenotomy – blunt, curved Westcott tenotomy – blunt, curved Vannas – curved with a sharp tip Right and Left Corneal section scissors Intraocular Needle holders- Barraquer type delicate and fine curved, non-locking, rounded and knurled handle Lens dialer/manipulator Phaco chopper Calipers- Jameson, Castroviejo Lens loop Irrigating cannulas – 21, 23, 25, 30 gauge Beaver blade handles Martinez corneal dissector Serrefine clamps Cyclodialysis spatual or iris spatula 1 3

5

2 4 6

9 7

8 Fundamentals of Microsurgery

 Instrumentation  Incisions  Incisions must be precise, accurate and atraumatic  Avoid “crush” techniques  Blade vs scissors  In general blades initiate corneal/adnexal incisions and scissors may complete the incision  Scissors often used alone for conjunctival incisions Fundamentals of Microsurgery

 Instrumentation  Incisions  Eyelid  May use a chalzion clamp or eyelid plate for stability and hemostatsis Fundamentals of Microsurgery  Instrumentation  Incisions  Cornea  Position forceps as close to incision as possible  Cut towards or away from forceps  Re-position the forceps as little as possible  If entering the anterior chamber incision depth ≥75%  Uni vs multi plane incisions  We use a 3.2mm biplanar incision for phaco Superficial Keratectomy

Cadaver surgery

What is the mistake? Lamellar Dissection Equine Corneal FB Corneal Entry

What is the mistake? Corneal Entry

Two-step incision Fundamentals of Microsurgery  Instrumentation  Incisions  Knives  Disposable vs re-useable  Bard Parker, Beaver, sapphire, diamond Fundamentals of Microsurgery Why does this incision not work well in our patients?

Fundamentals of Microsurgery  Instrumentation  Incisions  Scissors Fundamentals of Microsurgery  Instrumentation  Incisions  Scissors  Sharp vs blunt tip  Straight vs curved  Ringed, hinged, spring handled  Left and right directional  With or without a stop Fundamentals of Microsurgery  Instrumentation  Incisions  Scissors

Comments? Fundamentals of Microsurgery  Instrumentation  Incisions  Scissors

What is this and what is it for? Fundamentals of Microsurgery “when using scissors to cut tissue, avoid producing a serrated edge. Scissors are closed partially, reopened and carefully advanced in the original direction. Without removing the scissors, the blades are reapplied to the tissue” Fundamentals of Microsurgery  Instrumentation  Suture  Choices  absorbable vs. non-absorbable  braided vs. monofilament  size of the suture  size and type of needle  In general monofilament, non-absorbable is less reactive  Can remove absorbable in cornea to decrease reaction  Choose the smallest suture to achieve success  Avoid closure of instruments on suture whenever possible

Microsurgery

 Suture  Absorbable vs non-absorbable Microsurgery

 Suture  Absorbable vs non-absorbable  Monofilament vs braided  Swaged on needle essential  Suture size  6-0 to 11-0

Microsurgery

 Suture pattern  Interrupted vs continuous Microsurgery

 Suture  Absorbable vs non-absorbable  Monofilament vs braided  Swaged on needle essential Fundamentals of Microsurgery  Instrumentation  Suture  Size typically 6-0 to 10-0  volume of suture material left in the tissue increases logarithmically with increasing diameter  volume of material in 7-0 and 8-0 suture is 5x and 3x greater than in 9-0 respectively  Increased tissue reaction and knot size Suture material size based as defined by the United States Pharmacopoeia code

USP designation Collagen Absorbable Non-absorbable diameter (mm) diameter (mm) diameter (mm)

10-0 0.03 0.02 0.02 9-0 0.03 0.03 0.03 8-0 0.05 0.04 0.04 7-0 0.07 0.05 0.05 6-0 0.1 0.07 0.07 5-0 0.15 0.1 0.1 4-0 0.2 0.15 0.15

Suture gauge affects knot size more than # throws. Adding 2 additional knot throws increases mass by 1.5X while doubling suture gauge increases know volume by 4-6X. Fundamentals of Microsurgery  Instrumentation  Suture pattern  Surgeon preference  Astigmatism  Water tight seal  Interrupted, interlocking, continuous, double- continuous Fundamentals of Microsurgery  Instrumentation  Suture pattern  Interrupted  take longer to place  leave more knots  provide unidirectional tension vectors  more likely to tear out  Compressive or appositional effect of a simple interrupted suture is maximal only in the plane of the suture tract  Lateral to the suture tract the compressive effect diminishes  Lateral extent of the compression effect may be increased by increasing the size of the suture loop within the cornea Fundamentals of Microsurgery  Instrumentation  Suture pattern  Zones of compression seen with simple interrupted sutures. Note how the different length of the suture bites alters the zones of compression. Failure to achieve overlap of the compression zones will result in wound leakage. Fundamentals of Microsurgery  Instrumentation  Suture pattern  Simple continuous  Less time to place  Less knots  Results in lateral shifting of the wound  Suture break = dehiscence

Fundamentals of Microsurgery  Instrumentation  Suture pattern  Double continuous or counter suture pattern  takes slightly longer to place  leaves more suture material  advantages of even vector forces in all directions  better water-tight seal  less astigmatism  better wound integrity should a suture break occur Fundamentals of Microsurgery  Instrumentation  Suture pattern  A. A simple sawtooth suture pattern  B. A symmetrical sawtooth suture pattern  C. A double sawtooth suture pattern  D. A symmetrical double sawtooth suture pattern

Cat Claw Cat Claw

Seidel Test Seidel Test Positive Seidel Test - Canine Magnification Epinephrine Viscoelastic 8-0 to 9-0 suture Microsurgical instruments http://youtu.be/ex8EZVaP2aI Cat Claw Perforating with Lens capsule tear Cat Claw Perforating with Iris prolapse

Phacoanaphylaxis

Fundamentals of Microsurgery  Instrumentation  Suture pattern  Tied using instruments, preferably two tying forceps  Can use the tying platform on the colibri forceps for efficiency Fundamentals of Microsurgery  Instrumentation  Suture pattern  McPherson straight and angled  Right handed surgeon holds the straight forceps in their left hand  Left forceps wrap suture around the right Microsurgery

 Instrumentation  Suture forceps Over compression or poor instrument care may result is a failure to grasp the suture The first knotting loop is the “approximation loop” Additional loops secure the approximation loop Direction of tension applied will affect the type of knot created Microsurgery

 Instrumentation  Tissue forceps  Tying platform Tying platform Microsurgery

 Instrumentation  Specialized forceps

Colibri Utrata Microsurgery

 Instrumentation  Specialized forceps

2-4 um 50-70 um

Vannas Scissors

Utrata Forceps

Diathermy Shearing -safer, less radial tears, more control

Ripping -dangerous, more radial tears, less control The “Q” Sign

CCC – Vannas, Utrata The “Q” Sign Avoiding The “Q” Sign Avoiding The “Q” Sign Enlarge CCC post phaco Posterior capsulorhexis Fundamentals of Microsurgery  Instrumentation  Needles  Swaged on  Described by  curve of the needle  wire diameter  length of the needle  chord length, radius  shape of the needle in cross-section or point geometry  curve of microsurgical ophthalmic needle can be straight, 1/8, ¼, 3/8 and 1/2 circles Fundamentals of Microsurgery  Instrumentation  Needles  Corneal surgery needles with a 3/8 to 1/2 curve and a short 5-6mm length are most common  3/8 needle will result in a larger shallower bite while the ½ needle results in a short, deep bite Microsurgery

 Swaged on needle essential Fundamentals of Microsurgery  Instrumentation  Needles  point geometry is described as  taper (round needle with a taper point)  cutting (cuts on the inside curve)  reverse-cutting (cuts on the outside curve)  tapercut (round needle ending in a triangular cutting tip)  side cutting or spatula (flat top and bottom, cuts on the side)

Fundamentals of Microsurgery  Instrumentation  Needles  Cutting needles used for eyelid and adnexa  Spatula needles for cornea Microsurgery

 Needle  Swaged on  Curved  Taper  Cutting  Reverse cutting  Spatula

Fundamentals of Microsurgery  Instrumentation  Needle holder  Microneedle holders vary by  Size of the jaw (delicate, fine, medium, heavy)  Straight or curved jaw  Smooth or serrated jaw  Locking or non-locking  Style of the handle  Curved, non-locking, round handled Castroveijo or Barraquer most common Fundamentals of Microsurgery  Instrumentation  Needle holder  Must match the size of needle used  Grasp anterior to the midpoint Fundamentals of Microsurgery

I disagree with this, the needle will bend midshaft Microsurgery

 Instrumentation  Needle holders

Hold curved needle holders with the jaws curving upwards Fundamentals of Microsurgery  Instrumentation  Needle holder  When passing a needle, the curve of the needle holder is upwards  Tissue must be stabilized at the point of needle insertion  Use toothed forceps and if possible drive the needle towards the side with the most teeth – others disagree with this  Needle encounters the cornea perpendicular  Needle holder is rotated in the surgeon’s allowing the needle to follow its natural curve while the forceps elevate and open or evert the wound slightly  Forceps are used to stabilize, provide counter pressure and rotate the tissue as required to allow the needle to follow its path  Needle is grasped and re-grasped as it is advanced Fundamentals of Microsurgery  Instrumentation  Forceps  Basic functions of forceps are to  manipulate and stabilize ocular tissues  tying of sutures  removal of foreign bodies or distichia  performing a capsulorrhexis  IOL manipulation  With or without teeth  May or may not interdigitate  Teeth angle varies - ≥90 degrees  Size of teeth varies  +/- tying platform Fundamentals of Microsurgery  Instrumentation  Forceps  Size of teeth  Colibri  0.12 mm (cornea) or 0.3 mm and 0.5mm teeth (conjunctiva)  Bishop Harmon  eyelid (fine teeth) and conjunctiva (delicate teeth) Fundamentals of Microsurgery  Instrumentation  Forceps Fundamentals of Microsurgery  Instrumentation  Forceps Tools to help with capsulorhexis and sutured IOL

Duet® microsurgical tying forceps Microsurgical Technologies http://www.microsurgical.com/

Two-handed CTCC

4yr old terrier 2-handed CTCC

5yr old JRT other eye lost Modified ab-externo IOL

Wilkie DA, et al: A modified ab-externo approach for suture fixation of an intraocular IOL in the dog. Veterinary Ophthalmology 11:43-48, 2008 Dorsal suture placement - Duet® Forceps

Hemostasis

 Chemical  Epinephrine  Mechanical  Chalazion clamp  Wet field cautery  CO2 Laser Corneal Transplant

Corneal transplant Corneal transplant Corneal transplant Corneal transplant Penetrating Keratoplasty Penetrating Keratoplasty Penetrating Keratoplasty Penetrating Keratoplasty Penetrating Keratoplasty Pre-op

2yr Post-op 1 Days Postop

12 Days Postop 14 Days Postop

21 Days Postop

Viscoelastics

 Tools for spacial tactics  protect tissue and cells from mechanical trauma  create and preserve space for surgical manipulation  lubricate  separate tissues  prevent adhesions  tamponade hemorrhage  move or relocate tissue Visco over inflation –Iris Prolapse Viscoelastics Viscoelastics

 Viscoelastic substances must be sterile, nontoxic, nonpyrogenic, noninflammatory, and nonimmunogenic  Viscoelastics have properties of both fluids and solids  Described based on their rheologic properties of:  viscosity  pseudoplasticity  viscoelasticity  surface tension Viscoelastics

 Viscosity  Solutions resistance to flow  Concentration, molecular weight, size of the flexible random coils  Described in units of centipoise (cp)  Pseudoplasticity  With movement, viscosity decreases  Makes it easier to inject through a small cannula  Ideally a high viscosity at rest and this decreases as shear forces are applied Viscoelastics

 Viscoelasticity  Tendency to return to original shape  Surface tension  Lower surface tension = better ability to adhere or coat tissues Viscoelastics  Dispersive vs. Cohesive Viscoelastics  Dispersive  Consistency of molasses  Coat tissues well  Harder to remove  Cohesive  More solid than liquid  Do not coat or flow well  Maintain space better  Move tissues better  Easier to remove  Can use both – put dispersive in first Viscoelastics

 Na Hyaluronate  Rooster combs, umbilical cords, bacterial fermentation

 Chondroition sulfate  Shark fin cartilage

 Hydroxypropylmethylcellulose (HPMC)  Made from wood pulp and may contain particulate material Vitrectomy

 Oscillating  Single vs double cut  Guillotine  Pneumatic  Best for veterinary ophthalmology  Disposable best

Manual Vitrectomy Guillotine

Oscillating Single port - bad design Two port - best design What parameters can you control/adjust? Single port

X Bimanual Oscillating Guillotine Vitrectomy Posterior segment vitrectomy more complicated

Rabbit Fundamentals of Microsurgery

 Instrumentation  Arrange instruments in order of use  Replace in the same order Fundamentals of Microsurgery

 Instrumentation  Instrument cleaning and storage Fundamentals of Microsurgery

 Instrumentation  Instrument cleaning and storage  Distilled water  Delicate tooth brush/microwipes  Avoid direct instrument to instrument contact  Spray with instrument milk  Place in tray with lid  ALL SURGEONS ARE RESPONSIBLE FOR INSTRUMENT CARE Fundamentals of Microsurgery

 Instrumentation  Instrument cleaning and storage  Instruments with a lumen can be air blown and must be autoclaved NOT gassed Should you re-use your materials? Sterilization

 Do Not gas your tubing or handpieces  Gas does not sterilize areas that are wet or have trapped liquid  Clean after each use  Distilled water  Steam autoclave  Routine  Flash Fundamentals of Microsurgery

 Additional instruments  Corneal trephines  Cryosurgical unit  Phaco unit  Vitrectomy unit  Wet field cautery  Diode laser  Indirect  Transscleral  Intraocular  Microscope  ECP  Fluid-gas exchange pump  CO2 laser As we seek to improve we must all follow the basic rules: to use appropriate magnification and instrumentation, to be efficient and precise, to ensure minimal tissue trauma, to minimize surgical time, to maintain the anterior chamber using small incisions and viscoelastic materials, to obtain excellent tissue wound apposition with the smallest and most appropriate suture materials and finally, to achieve a successful, comfortable, cosmetic and whenever possible, visual outcome We must remember that microsurgery is both a technique and an art and as such we must constantly work to refine and improve ourselves as microsurgeons. We must also remain open to new ideas and respect the opinions of others who may have a different method to achieve success. Intraocular Surgery

 “The enemy of GOOD is BETTER” Questions?