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Morphological Characterization and Dna
MORPHOLOGICAL CHARACTERIZATION AND DNA FINGERPRINTING OF A PRASINOPHYTE FLAGELLATE ISOLATED FROM KERLA COAST BY KANCHAN SHASHIKANT NASARE DIVISION OF BIOCHEMICAL SCIENCES NATIONAL CHEMICAL LABORATORY PUNE-411008, INDIA 2002 MORPHOLOGICAL CHARACTERIZATION AND DNA FINGERPRINTING OF A PRASINOPHYTE FLAGELLATE ISOLATED FROM KERALA COAST A THESIS SUBMITTED TO THE UNIVERSITY OF PUNE FOR THE DEGREE OF DOCTOR OF PHILOSOPHY (IN BOTANY) BY KANCHAN SHASHIKANT NASARE DIVISION OF BIOCHEMICAL SCIENCES NATIONAL CHEMICAL LABORATORY PUNE-411008, INDIA. October 20002 DEDICATED TO MY FAMILY TABLE OF CONTENTS Page No. Declaration I Acknowledgement II Abbreviations III Abstract IV-VIII Chapter 1: General Introduction 1-19 Chapter2: Morphological characterization of a prasinophyte 20-43 flagellate isolated from Kochi backwaters Abstract 21 Introduction 21 Materials and Methods 23 Materials 23 Methods 23 Growth medium 23 Optimization of culture conditions 25 Pigment analysis 26 Light and electron microscopy 26 Results and Discussion Culture conditions 28 Pigment analysis 31 Light microscopy 32 Scanning electron microscopy 35 Transmission electron microscopy 36 Chapter 3: Phylogenetic placement of the Kochi isolate 44-67 among prasinophytes and other green algae using 18S ribosomal DNA sequences Abstract 45 Introduction 45 Materials and Methods 47 Materials 47 Methods 47 DNA isolation 47 Amplification of 18S rDNA 48 Sequencing of 18S rDNA 48 Sequence analysis 49 Results and Discussion 50 Chapter 4: DNA fingerprinting of the prasinophyte 68-101 flagellate isolated -
A Review on Presence of Oleanolic Acid in Natural Products
Natura Proda Medica, (2), April 2009 64 A review on presence of Oleanolic acid in Natural Products A review on presence of Oleanolic acid in Natural Products YEUNG Ming Fai Abstract Oleanolic acid (OA), a common phytochemical, is chosen as an example for elucidation of its presence in natural products by searching scientific databases. 146 families, 698 genera and 1620 species of natural products were found to have OA up to Sep 2007. Keywords Oleanolic acid, natural products, plants, Chinese medicine, Linnaeus system of plant classification Introduction and/or its saponins in natural products was carried out for Oleanolic acid (OA), a common phytochemical, is chosen elucidating its pressence. The classification was based on as an example for elucidation of its presence in natural Linnaeus system of plant classification from the databases of products by searching scientific databases. SciFinder and China Yearbook Full-text Database (CJFD). Methodology of Review Result of Review Literature search for isolation and characterization of OA Search results were tabulated (Table 1). Table 1 Literature review of natural products containing OA and/or its saponins. The classification is based on Angiosperm Phylogeny Group APG II system of plant classification from the databases of SciFinder and China Yearbook Full-text Database (CJFD). Family of plants Plant scientific names Position of plant to be Form of OA References isolated isolated Acanthaceae Juss. Acanthus illicifolius L. Leaves OA [1-2] Acanthaceae Avicennia officinalis Linn. Leaves OA [3] Acanthaceae Blepharis sindica Stocks ex T. Anders Seeds OA [4] Acanthaceae Dicliptera chinensis (Linn.) Juss. Whole plant OA [5] Acanthaceae Justicia simplex Whole plant OA saponins [6] Actinidiaceae Gilg. -
History and Current Status of Systematic Research with Araceae
HISTORY AND CURRENT STATUS OF SYSTEMATIC RESEARCH WITH ARACEAE Thomas B. Croat Missouri Botanical Garden P. O. Box 299 St. Louis, MO 63166 U.S.A. Note: This paper, originally published in Aroideana Vol. 21, pp. 26–145 in 1998, is periodically updated onto the IAS web page with current additions. Any mistakes, proposed changes, or new publications that deal with the systematics of Araceae should be brought to my attention. Mail to me at the address listed above, or e-mail me at [email protected]. Last revised November 2004 INTRODUCTION The history of systematic work with Araceae has been previously covered by Nicolson (1987b), and was the subject of a chapter in the Genera of Araceae by Mayo, Bogner & Boyce (1997) and in Curtis's Botanical Magazine new series (Mayo et al., 1995). In addition to covering many of the principal players in the field of aroid research, Nicolson's paper dealt with the evolution of family concepts and gave a comparison of the then current modern systems of classification. The papers by Mayo, Bogner and Boyce were more comprehensive in scope than that of Nicolson, but still did not cover in great detail many of the participants in Araceae research. In contrast, this paper will cover all systematic and floristic work that deals with Araceae, which is known to me. It will not, in general, deal with agronomic papers on Araceae such as the rich literature on taro and its cultivation, nor will it deal with smaller papers of a technical nature or those dealing with pollination biology. -
The Marine Macroalgae of Cabo Verde Archipelago: an Updated Checklist
Arquipelago - Life and Marine Sciences ISSN: 0873-4704 The marine macroalgae of Cabo Verde archipelago: an updated checklist DANIELA GABRIEL AND SUZANNE FREDERICQ Gabriel, D. and S. Fredericq 2019. The marine macroalgae of Cabo Verde archipelago: an updated checklist. Arquipelago. Life and Marine Sciences 36: 39 - 60. An updated list of the names of the marine macroalgae of Cabo Verde, an archipelago of ten volcanic islands in the central Atlantic Ocean, is presented based on existing reports, and includes the addition of 36 species. The checklist comprises a total of 372 species names, of which 68 are brown algae (Ochrophyta), 238 are red algae (Rhodophyta) and 66 green algae (Chlorophyta). New distribution records reveal the existence of 10 putative endemic species for Cabo Verde islands, nine species that are geographically restricted to the Macaronesia, five species that are restricted to Cabo Verde islands and the nearby Tropical Western African coast, and five species known to occur only in the Maraconesian Islands and Tropical West Africa. Two species, previously considered invalid names, are here validly published as Colaconema naumannii comb. nov. and Sebdenia canariensis sp. nov. Key words: Cabo Verde islands, Macaronesia, Marine flora, Seaweeds, Tropical West Africa. Daniela Gabriel1 (e-mail: [email protected]) and S. Fredericq2, 1CIBIO - Research Centre in Biodiversity and Genetic Resources, 1InBIO - Research Network in Biodiversity and Evolutionary Biology, University of the Azores, Biology Department, 9501-801 Ponta Delgada, Azores, Portugal. 2Department of Biology, University of Louisiana at Lafayette, Lafayette, Louisiana 70504-3602, USA. INTRODUCTION Schmitt 1995), with the most recent checklist for the archipelago published in 2005 by The Republic of Cabo Verde is an archipelago Prud’homme van Reine et al. -
Updates in the Flora of the Maltese
A FLORISTIC SURVEY ON THE GOZITAN ISLETS OF TAĊ-ĊAWL AND TAL-ĦALFA IN THE MALTESE ARCHIPELAGO Stephen Mifsud Citation: Mifsud, S. (2011).A FLORISTIC SURVEY ON THE GOZITAN ISLETS OF TAC-CAWL AND TAL-HALFA IN THE MALTESE ISLANDS". MaltaWildPlants.com Online Publications; Ref: MWPOP-001. URL: http://www.MaltaWildPlants.com/publ/index.php#W01 Abstract A floristic survey was carried out on the islets of Tal-Ħalfa and Taċ-Ċawl, both situated just off the Southern coast of Gozo. The aim was to confirm or otherwise results of a recent study (Sciberras & Sciberras, 2010) which concluded that a number of species had become extinct or doubtful when compared to an earlier work (Cassar and Lanfranco, 2000). The present work confirms the occurrence of most of the species found by Cassar and Lanfranco and in addition reports on the discovery of a significant number of new records of legally protected or rare vascular plants. Taxonomical notes concerning a few ambiguous plants which were discovered are discussed. Keywords Flora, Taċ-Ċawl, Tal-Ħalfa, Islets, Maltese Islands 1: Introduction After reading an article (Sciberras & Sciberras, 2010) regarding surveys carried out on the islets around the Maltese islands, the author decided to visit and survey the flora of the islets of Taċ-Ċawl and Tal-Ħalfa. The reason being that of a substantial number of discrepancies between this article and an earlier survey (Cassar and Lanfranco, 2000). It was surprising to read that after visiting each site at least “four times each season from the period 1998-2010” the authors had only found 24 and 21 species for Taċ-Ċawl and Tal-Ħalfa Rocks respectively. -
Universidade De Coimbra
UNIVERSIDADE DE COIMBRA FACULDADE DE CIÊNCIAS E TECNOLOGIA Estudos de Etnobotânica e Botânica Económica no Alentejo Luís Manuel Mendonça de Carvalho 2006 UNIVERSIDADE DE COIMBRA FACULDADE DE CIÊNCIAS E TECNOLOGIA Estudos de Etnobotânica e Botânica Económica no Alentejo Luís Manuel Mendonça de Carvalho Dissertação de Doutoramento em Biologia - Sistemática e Morfologia, apresentada à Faculdade de Ciências e Tecnologia da Universidade de Coimbra, sob orientação da Professora Doutora Maria Teresa Fernandes de Almeida 2006 Luís Manuel Mendonça de Carvalho UNIVERSIDADE DE COIMBRA FACULDADE DE CIÊNCIAS E TECNOLOGIA 2006 Estudos de Etnobotânica e Botânica Económica no Alentejo A vida tranquila e a sabedoria conservam-se ao abrigo do desgaste e garantem a sua duração. Por muito longe que os deuses habitem no Éter, eles vêem as obras dos mortais. As Bacantes (Coro) Eurípides I II iis qui me amant, et in terra et in caelo Francisca Ana António Ana Maria Teófilo Maria Águeda III IV Agradecimentos À Professora Doutora Maria Teresa Fernandes de Almeida Aos Informantes que nos permitiram registar os seus conhecimentos etnobotânicos À Francisca Maria Ao Professor Doutor Diego Rivera Núñez, Universidade de Murcia À Professora Doutora Margarita Costa Tenorio, Universidade Complutense de Madrid À Professora Doutora Alpina Begossi, Universidade Estadual de Campinas Este estudo foi parcialmente financiado pelo Programa PRODEP (Medida 5, Acção 5.3) V VI Sinopse As actuais circunstâncias económicas e sociais conduzem o conhecimento de matriz etnobotânica a um inexorável processo de extinção, porque são os cidadãos mais idosos os seus depositários. Com a sua eventual perda, associada ao fim das práticas agrícolas tradicionais, desaparecerão informações protocientíficas acumuladas ao longo de séculos. -
Molecular Characterization of Eukaryotic Algal Communities in The
Zhu et al. BMC Plant Biology (2018) 18:365 https://doi.org/10.1186/s12870-018-1588-7 RESEARCH ARTICLE Open Access Molecular characterization of eukaryotic algal communities in the tropical phyllosphere based on real-time sequencing of the 18S rDNA gene Huan Zhu1, Shuyin Li1, Zhengyu Hu2 and Guoxiang Liu1* Abstract Backgroud: Foliicolous algae are a common occurrence in tropical forests. They are referable to a few simple morphotypes (unicellular, sarcinoid-like or filamentous), which makes their morphology of limited usefulness for taxonomic studies and species diversity assessments. The relationship between algal community and their host phyllosphere was not clear. In order to obtain a more accurate assessment, we used single molecule real-time sequencing of the 18S rDNA gene to characterize the eukaryotic algal community in an area of South-western China. Result: We annotated 2922 OTUs belonging to five classes, Ulvophyceae, Trebouxiophyceae, Chlorophyceae, Dinophyceae and Eustigmatophyceae. Novel clades formed by large numbers sequences of green algae were detected in the order Trentepohliales (Ulvophyceae) and the Watanabea clade (Trebouxiophyceae), suggesting that these foliicolous communities may be substantially more diverse than so far appreciated and require further research. Species in Trentepohliales, Watanabea clade and Apatococcus clade were detected as the core members in the phyllosphere community studied. Communities from different host trees and sampling sites were not significantly different in terms of OTUs composition. However, the communities of Musa and Ravenala differed from other host plants significantly at the genus level, since they were dominated by Trebouxiophycean epiphytes. Conclusion: The cryptic diversity of eukaryotic algae especially Chlorophytes in tropical phyllosphere is very high. -
) 2 10( ;3 201 Life Science Journal 659
Science Journal 210(;3201Life ) http://www.lifesciencesite.com Habitats and plant diversity of Al Mansora and Jarjr-oma regions in Al- Jabal Al- Akhdar- Libya Abusaief, H. M. A. Agron. Depar. Fac. Agric., Omar Al-Mukhtar Univ. [email protected] Abstract: Study conducted in two areas of Al Mansora and Jarjr-oma regions in Al- Jabal Al- Akhdar on the coast. The Rocky habitat Al Mansora 6.5 km of the Mediterranean Sea with altitude at 309.4 m, distance Jarjr-oma 300 m of the sea with altitude 1 m and distance. Vegetation study was undertaken during the autumn 2010 and winter, spring and summer 2011. The applied classification technique was the TWINSPAN, Divided ecologically into six main habitats to the vegetation in Rocky habitat of Al Mansora and five habitats in Jarjr oma into groups depending on the average number of species in habitats and community: In Rocky habitat Al Mansora community vegetation type Cistus parviflorus, Erica multiflora, Teucrium apollinis, Thymus capitatus, Micromeria Juliana, Colchium palaestinum and Arisarum vulgare. In Jarjr oma existed five habitat Salt march habitat Community dominant species by Suaeda vera, Saline habitat species Onopordum cyrenaicum, Rocky coastal habitat species Rumex bucephalophorus, Sandy beach habitat species Tamarix tetragyna and Sand formation habitat dominant by Retama raetem. The number of species in the Rocky habitat Al Mansora 175 species while in Jarjr oma reached 19 species of Salt march habitat and Saline habitat 111 species and 153 of the Rocky coastal habitat and reached to 33 species in Sandy beach and 8 species of Sand formations habitat. -
Potential Toxicity of Medicinal Plants Inventoried in Northeastern Morocco: an Ethnobotanical Approach
plants Article Potential Toxicity of Medicinal Plants Inventoried in Northeastern Morocco: An Ethnobotanical Approach Loubna Kharchoufa 1, Mohamed Bouhrim 1, Noureddine Bencheikh 1 , Mohamed Addi 2 , Christophe Hano 3 , Hamza Mechchate 4,* and Mostafa Elachouri 1 1 Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, URAC-40, Department of Biology, Faculty of Sciences, Mohammed First University, Oujda 60040, Morocco; [email protected] (L.K.); [email protected] (M.B.); [email protected] (N.B.); [email protected] (M.E.) 2 Laboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement, (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda 60000, Morocco; [email protected] 3 Laboratoire de Biologie des Ligneux et des Grandes Cultures, INRAE USC1328, Campus Eure et Loir, Orleans University, 45067 Orleans, France; [email protected] 4 Laboratory of Biotechnology, Environment, Agrifood and Health, Faculté des Sciences Dhar el Mahraz, University of Sidi Mohamed Ben Abdellah, Fez 30050, Morocco * Correspondence: [email protected] Abstract: Herbal medicine and its therapeutic applications are widely practiced in northeastern Morocco, and people are knowledgeable about it. Nonetheless, there is a significant knowledge gap regarding their safety. In this study, we reveal the toxic and potential toxic species used as medicines by people in northeastern Morocco in order to compile and document indigenous knowl- Citation: Kharchoufa, L.; edge of those herbs. Structured and semi-structured interviews were used to collect data, and Bouhrim, M.; Bencheikh, N.; simple random sampling was used as a sampling technique. Based on this information, species Addi, M.; Hano, C.; Mechchate, H.; were collected, identified, and herbarium sheets were created. -
Analysis of the Vascular Flora of Four Satellite Islets of the Egadi Archipelago (W Sicily), with Some Notes on Their Vegetation and Fauna
Biodiversity Journal, 2014, 5 (1): 39–54 Analysis of the vascular flora of four satellite islets of the Egadi Archipelago (W Sicily), with some notes on their vegetation and fauna Salvatore Pasta1*, Arnold Sciberras2, Jeffrey Sciberras3 & Leonardo Scuderi4 1National Research Council (CNR), Istitute of Biosciences and Bioresources (IBBR), Corso Calatafimi, 414 - 90129, Palermo, Italy; e-mail: [email protected] 2133, Arnest, Arcade Str., Paola, Malta; e-mail: [email protected] 324 Camilleri crt Flt 5,Triq il-Marlozz, Ghadira, Mellieha, Malta; e-mail: [email protected] 4via Andromaca, 60 - 91100 Trapani, Italy; e-mail: [email protected] *Corresponding author ABSTRACT This paper represents the first contribution on the vascular flora of the stack named Faraglione di Levanzo and of three satellite islets of Favignana, i.e. Prèveto, Galeotta and a stack located at Cala Rotonda. A sketch of their vegetation pattern is also provided, as well as a list of all the terrestrial fauna, with some more detailed information on the vertebrates. The finding of some bones of Mustela nivalis Linnaeus, 1758 is the first record for the whole archipelago and deserves further investigations. The floristic data have been used in order to analyze life-form and chorological spectra and to assess species-area relationship, the peculiarity of local plant assemblages, the occurrence of islet specialists, the risk of alien plants invasion and the refugium role played by the islets. The significant differences among the check-lists compiled by the two different couples of authors during their own visits to Prèveto and Galeotta underline the need of planning regular and standardized field investigations in order to avoid an overestimation of local species turnover rates. -
Towards Integrated Marine Research Strategy and Programmes CIGESMED
Towards Integrated Marine Research Strategy and Programmes CIGESMED : Coralligenous based Indicators to evaluate and monitor the "Good Environmental Status" of the Mediterranean coastal waters French dates: 1st March2013 -29th October2016 Greek dates: 1st January2013 -31st December2015 Turkish dates: 1st February2013 –31st January2016 FINAL REPORT Féral (J.-P.)/P.I., Arvanitidis (C.), Chenuil (A.), Çinar (M.E.), David (R.), Egea (E.), Sartoretto (S.) 1 INDEX 1. Project consortium. Total funding and per partner .............................................................. 3 2. Executive summary ............................................................................................................... 3 3. Aims and scope (objectives) .................................................................................................. 6 4. Results by work package ....................................................................................................... 8 WP1: MANAGEMENT, COORDINATION & REPORTING ............................................................. 8 WP2: CORALLIGEN ASSESSMENT AND THREATS ..................................................................... 15 WP3: INDICATORS DEVELOPMENT AND TEST ......................................................................... 39 WP4: INNOVATIVE MONITORING TOOLS ................................................................................ 52 WP5: CITIZEN SCIENCE NETWORK IMPLEMENTATION ........................................................... 58 WP6: DATA MANAGEMENT, MAPPING -
Floristic Account of the Marine Benthic Algae from Jarvis Island and Kingman Reef, Line Islands, Central Pacific
Micronesica 43(1): 14 – 50, 2012 Floristic Account of the Marine Benthic Algae from Jarvis Island and Kingman Reef, Line Islands, Central Pacific Roy T. Tsuda, Jack R. Fisher1 Department of Natural Sciences, Bishop Museum, 1525 Bernice Street, Honolulu, HI 96817-2704, USA. [email protected] and Peter S. Vroom Ocean Associates, NOAA Fisheries Pacific Islands Fisheries Science Center, Coral Reef Ecosystem Division, 1125B Ala Moana Boulevard, Honolulu, HI 96814, USA. Abstract—The marine benthic algae from Jarvis Island and Kingman Reef were identified from collections obtained from the Whippoor- will Expedition in 1924, the Itasca Expedition in 1935, the U.S. Coast Guard Cutter Taney in 1938, the Smithsonian Institution’s Pacific Ocean Biological Survey Program in 1964 and the U.S. National Oceanic and Atmospheric Administration’s Reef Assessment and Monitoring Program (RAMP) in 2000, 2001, 2002, 2004 and 2006. A total of 124 species, representing 8 Cyanobacteria (blue-green algae), 82 Rhodophyta (red algae), 6 Heterokontophyta (brown algae) and 28 Chlorophyta (green algae), are reported from both islands. Seventy-nine and 95 species of marine benthic algae are recorded from Jarvis Island and Kingman Reef, respectively. Of the 124 species, 77 species or 62% (4 blue-green algae, 57 red algae, 2 brown algae and 14 green algae) have never before been reported from the 11 remote reefs, atolls and low islands comprising the Line Islands in the Central Pacific. Introduction The Line Islands are located approximately 1600 kilometers south of the main Hawaiian Islands and consist of 11 remote reefs, atolls and low islands that lie between 7° N and 12° S latitude, and 150° W and 164° W longitude (Fig.