ISSN 10227954, Russian Journal of Genetics, 2014, Vol. 50, No. 3, pp. 253–260. © Pleiades Publishing, Inc., 2014. Original Russian Text © E.A. Vasyutkina, G.D. Reunova, A.E. Tupikin, Yu.N. Zhuravlev, 2014, published in Genetika, 2014, Vol. 50, No. 3, pp. 291–298. PLANT GENETICS Mitochondrial DNA Variation in Olga Bay Larch (Larix olgensis A. Henry) from Primorsky Krai of Russia E. A. Vasyutkinaa, G. D. Reunovaa, A. E. Tupikinb, and Yu. N. Zhuravleva aInstitute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022 Russia email:
[email protected] bInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090 Russia Received August 8, 2013; in final form, October 10, 2013 Abstract—Two mitochondrial DNA fragments, nad4(3c4r) and nad5(12r), were sequenced in 58 larch accessions from the range of Larix olgensis A. Henry. Combinations of the nad4 polymorphic sites formed four haplotypes, two of which (H3 and H4) were unique and two (H1, H2) were common. Haplotype H1 was found only in pure L. olgensis from the vicinity of Olga Bay and in a number of accessions from the southern part of the range. Haplotype H2 was detected in the other samples from the range of Olga Bay larch, as well as in hybrid forms. Similarly to the nad4(3c4r) fragment, the mtDNA fragment UBC460 was able to differ entiate larch populations from the range of L. olgensis examined. DOI: 10.1134/S1022795414030107 INTRODUCTION species (L. lubarskii, L. komarovii, L. amurensis, L. ochotensis, L.