01 Contents Food Sheet No

CONTENTS 01 CONTENTS FOOD SHEET NO. TITLE PAGE SHEET NO. TITLE PAGE

F001 Separation of Water-soluble Vitamins - 1 05 F069 Separation of Acesulfame K 25 F002 Separation of Water-soluble Vitamins - 2 05 F080 Separation of Aspartame and Acesulfame K in breath mint 25 F067 Separation of Vitamins 06 F049 Separation of Saccharin & Sorbic Acid 25 F005 Separation of Fat-soluble Vitamins 06 F076 Separation of Cyclamic acid 25 F070 Separation of Vitamin C 07 F051 Separation of Pyrazines 26 F003 Separation of Vitamin D2, D3 07 F089 Separation of Ubiquinone 9,10 26 F057 Separation of Tocopherols 07 F090 Separation of DL-Thioctic acid 26 F058 Separation of Vitamin C and E 08 F091 Separation of Benzoyl Peroxide in Flour 26 F097 Separation of Vitamin B12 in Health food 08 F053 Separation of Caffeine and Catechins 27 F071 Separation of Carotenoids 08 F054 Separation of Commercial Tea Drink 27 F004 Separation of β-Carotene in Broccoli 09 F055 Separation of Catechins 28 F059 Separation of β-Carotene 09 F056 Separation of Catechins, Caffeine, Chlorophyll a 28 F007 Separation of Synthetic Antibiotics (Quinolone Derivatives)-1 09 F063 Separation of Allyl isothiocyanate 29 F008 Separation of Synthetic Antibiotics (Quinolone Derivatives)-2 10 F064 Separation of Quassins 29 F009 Separation of Synthetic Antibiotics (Sulfa drugs) 10 F065 Separation of Capsaicins 29 F010 Separation of Synthetic Antibiotics (Furan Derivatives) 11 F082 Separation of Curcumin 29 F011 Separation of Synthetic Antibiotics (Protozoicides) 11 F083 Separation of Curcumin in a commercial drink by LC/MS 30 F012 Separation of Synthetic Antibiotics (Protozoicide & Quinolone) 11 F078 Separation of Rutin and Quercetin 30 F013 Separation of 18 Synthetic Antibiotics 12 F099 Separation of Apigenin 30 F026 Separation of Carbadox 13 F100 Separation of 5,6-Benzoflavone 30 F027 Separation of a Metabolite of Carbadox 13 F101 Separation of 7,8-Benzoflavone 31 F028 Separation of Sulfadimidine 13 F102 Separation of Biochanin A 31 F006 Separation of Tetracycline Antibiotics 13 F103 Separation of Chrysin 31 F014 Separation of Drugs for Animals -1 14 F104 Separation of Diosmin 31 F015 Separation of Drugs for Animals -2 14 F105 Separation of Flavonol 32 F019 Analysis of Oxytetracyclne in Pork 14 F106 Separation of Hesperetin 32 F016 Analysis of α-Trenbolone in Pork 15 F107 Separation of Hesperidin 32 F017 Analysis of β-Trenbolone in Pork 15 F108 Separation of Isoquercitrin 32 F021 Analysis of Zeranol in Pork 15 F109 Separation of Kaempferol 33 F018 Analysis of lvermectin in Pork 15 F110 Separation of Luteolin 33 F020 Analysis of Closantel in Pork 16 F111 Separation of Naringenin 33 F022 Analysis of Flubendazole in Pork 16 F112 Separation of Naringin 33 F029 Separation of Thiabendazole 16 F113 Separation of Rhoifolin 34 F030 Separation of a Metabolite of Thiabendazole 16 F114 Separation of 4,2',4'-Trihydroxychalcone 34 F023 Separation of Albendazole 17 F066 Separation of Oligosaccharide 34 F024 Separation of a Metabolite of Albendazole 17 F068 Separation of Carbohydrates 34 F025 Separation of Isomethamidium 17 F032 Separation of di-Carbonic Acids - 1 35 F031 Separation of Avoparcins 17 F092 Separation of di-Carbonic Acids - 2 35 F044 Separation of Preservatives - 1 18 F035 Separation of Organic Acids - 1 35 F047 Separation of Preservatives - 2 18 F093 Separation of Organic Acids – 2 35 F075 Separation of Preservatives - 3 18 F036 Separation of Organrc Acids - 3 36 F043 Separation of Preservatives - 4 18 F094 Separation of Organic Acids - 4 36 F074 Separation of Preservatives - 5 19 F098 Separation of Organic Acids - 5 36 F077 Separation of Preservatives - 6 19 F062 Separation of Formic acid and Tartaric acid 36 F061 Separation of Preservatives and Saccharin 20 F033 Separation of Oxalic Acid and Nitric Acid in Spinach 37 F042 Separation of Antioxidants - 1 20 F034 Separation of Aromatic Acids -1 37 F073 Separation of Antioxidants - 2 20 F096 Separation of Aromatic Acids -2 37 F048 Separation of Ascorbic Acid & Erythorbic Acid 21 F095 Separation of Aromatic Acids -3 37 F045 Separation of anti-Fungal Preservatives - 1 21 F084 Separation of Malachite green and Leucomalachite green 38 F046 Separation of anti-Fungal Preservatives - 2 21 F085 Separation of Malachite green and Leucomalachite green by 38 F037 Separation of Food Dyes - 1 21 LC/MS F038 Separation of Food Dyes - 2 22 F086 Separation of Sudan 38 F039 Separation of Food Dyes - 3 22 F087 Separation of Sudan by LC/MS 39 F040 Separation of Food Dyes - 4 23 F052 Separation of Aflatoxin G1, G2, B1, B2 39 F041 Separation of Food Dyes - 5 23 F081 Separation of Fumonisins 40 F072 Separation of Food Dyes - 6 24 F088 Separation of Melamine 40 F060 Separation of Anthocyanins 24 F079 Separation of Putrefactive amines 40 F050 Separation of Aspartame in a Beverage 24

CONTENTS 01 CONTENTS ENVIRONMENT SHEET NO. TITLE PAGE

E001 Separation of N-Methylcarbamate derivatives- 1 43 E002 Separatiori of N-Methylcarbamate derivatives- 2 43 E003 Separatron of Oxine-Copper and Asulam 44 E004 Separation of Oxine-Copper and Thiram 44 E005 Separation of Simazine, Thiram and Thiobencarb 44 E006 Separation of Thiram, Iprodione and Bensulide 44 E007 Separation of Thiram 45 E008 Separation of Thiophanate-methyl and its metabolite 45 E009 Separation of Fenitrothion and its metabolite 45 E011 Separation of Agricultural Chemicals (Germicides) 45 E012 Separation of Agricultural Chemicals (Pesticides) 46 E013 Separation of Agricultural Chemicals (Herbicides)- 1 46 E014 Separation of Agricultural Chemicals (Herbicides)- 2 46 E015 Separation of 5 Agricultural Chemicals - 1 46 E016 Separation of 5 Agricultural Chemicals - 2 47 E028 Separation of 5 Agricultural Chemicals - 3 47 E029 Separation of 5 Agricultural Chemicals - 4 47 E034 Separation of 5 Agricultural Chemicals - 5 47 E017 Separation of 6 Agricultural Chemicals 48 E018 Separation of 9 Agricultural Chemicals - 1 48 E030 Separation of 12 Agricultural Chemicals 48 E035 Separation of 9 Agricultural Chemicals - 2 49 E036 Separation of 3 Agricultural Chemicals 49 E045 Separation of Pesticides standard solution 24【A golf course 50 pesticide】

E019 Separation of Intermediates of Agricultural Chemicals 50 (halogenized Benzoic acids) E027 Separation of Carbofuran 50 E032 Separation of Acephate 51 E033 Separation of Imazalil in Concentrated Lemonade Juice 51 E040 Separation of Pesticides standard solution 28 51 E041 Separation of 3 Agricultural Chemicals 51 E042 Separation of Carbaryl 52 E043 Separation of Diquat 52 E044 Separation of Iminoctadine triacetate 52 E039 Separation of LAS 52 E010 Separation of 15 Synthetic Antibiotics 53 E020 Separation of Benzo[a]pyrene 53 E023 Separation of Microsystins 53 E046 Separation of Patulin 54 E024 Separation of Bisphenol A -1 54 E025 Separation of Bisphenol A -2 54 E026 Separation of Trace Level Bisphenol A 54 E031 Separation of 4-Vinylguaiacol 55 E021 Separation of DNPH-Aldehydes-1 55 E022 Separation of DNPH-Aldehydes-2 55 E037 Separation of DNPH-Aldehydes-3 56 E038 Separation of DNPH-Aldehydes-4 56

Concerning Mightysil RP-18 GP series All columns with the "GP" title include the batch certificate. The guaranteed content is as follows. 1. The physical properties of the packing materials, and carbon content after bonding 2. Analytical results and Test-chromatograms of a separation with our own standard of test-mixtures

Difference in entitling "GP"or not is only including a batch certificate or not. In addition, they are made with the same packing material.

02 CONTENTS FOOD 03 04 FOOD F001 Separation of Water-soluble Vitamins - 1

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ 20mM Phosphoric acid including 25mM of Sodium hexanesulfonate = 10/ 90 Flow Rate 1.0mL/min. Detection UV210nm 0.16AUFS Temperature 40°C Sample Vol. 5μL Samples 1. Nicotinic acid 50μg/mL 2. Nicotinamide 50μg/mL 3. Riboflavin phosphate sodium salt 50μg/mL

4. Pyridoxine hydrochloride (Vitamin B6) 200μg/mL

5. Riboflavin (Vitamin 2B ) 150μg/mL

6. Folic acid (Vitamin B9) 50mg/mL

7. Thiamin hydrochloride(Vitamin B1) 150μg/mL

F002 Separation of Water-soluble Vitamins - 2

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ 20mM Phosphoric acid including 25mM of Sodium hexanesulfonate = 10/ 90 Flow Rate 1.0mL/min. Detection UV210nm 0.08AUFS Temperature 40°C Sample Vol. 5μL Samples 1. Pantothenic acid calcium salt 160μg/mL 2. Nicotinamide 20μg/mL

3. Pyridoxine hydrochloride (Vitamin B6) 40μg/mL

4. Riboflavin(Vitamin 2B ) 80μg/mL

FOOD 05 F067 Separation of Vitamins

Column Mightysil RP-18GP Aqua 250-4.6mmØ 5μm Eluent 0.1% Phosphoric acid containing 10mM Sodium hexanesulfonate/ Acetonitrile = 90/10 Flow Rate 1.0mL/min. Detection UV210nm Temperature 35°C Sample Vol. 5μL Samples 1. Nicotinic acid 0.05mg/mL 2. Pantothenic acid Calcium salt 0.3 mg/mL 3. Nicotinamide 0.05mg/mL 4. Riboflavin phosphate Sodium salt 0.2 mg/mL

5. Pyridoxine hydrochloride(VitaminB6) 0.05mg/mL

6. Riboflavin (VitaminB2) 0.15mg/mL

7. Folic acid (VitaminB9) 0.05mg/mL 8. Thiamine hydrochloride 0.15mg/mL

F005 Separation of Fat-soluble Vitamins

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ Methanol = 60/40 Flow Rate 1.0mL/min. Detection UV280nm 0.16AUFS Temperature 40ºC Sample Vol. 5μL

Samples 1. Menadione (Vitamin K3) 0.25mg/mL 2. Retinol acetate (Vitamin A acetate) 0.09mg/mL

3. Ergocalciferol (Vitamin D2) 0.10mg/mL

4. Cholecalciferol (Vitamin D3) 0.10mg/mL 5. Tocopherol (Vitamin E) 0.75mg/mL 6. Tocopherol acetate (Vitamin E acetate) 1.00mg/mL

7. Phylloquinone (Vitamin K1) 0.50mg/mL

06 FOOD F070 F003 Separation of Vitamin C Separation of Vitamin D2, D3

Column Mightysil RP-18 250-4.6mmØ 5μm Column Mightysil RP-18GP Aqua 150-4.6mmØ 5μm Eluent Acetonitrile/ Water = 95/ 5 Eluent 1% Methaphosphoric acid Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV210nm 0.08AUFS Detection UV242nm Temperature Room Temperature Temperature Ambient Sample Vol. 5μL

Sample Vol. 5μL Samples 1. Ergocalciferol (Vitamin D2) 250μg/mL

Samples 1. L-Ascorbic acid 50mg/mL 2. Cholecalciferol (Vitamin D3) 250μg/mL

F057 Separation of Tocopherols

Column Mightysil Si60 150-4.6mmØ 5μm Eluent Hexane/ Dioxane/ Isopropylalcohol = 97.5/ 2/ 0.5 Flow Rate 1.0mL/min. Detection Fluorescence 323nm (Excitation 296nm) Temperature Ambient Sample Vol. 5μL Samples 1. α-Tocopherol 1μg/mL 2. 2,2,5,7,8-Pentamethylhydroxychroman 1μg/mL 3. β-Tocopherol 1μg/mL 4. γ-Tocopherol 1μg/mL 5. δ-Tocopherol 1μg/mL

FOOD 07 F058 F097 Separation of Vitamin C and E Separation of Vitamin B12 in Health food

Column Mightysil RP-18GP 250-4.6mmØ 5μm Eluent A: Methanol/ 20mM Phosphoric acid = 1/ 99 B : Methanol/ Acetonitrile = 40/60 Gradient, Flow rate : 0~10min.: A100%, 1.0mL/min Column Mightysil RP-18GP Aqua 150-4.6mmØ 5μm 10.1~30min.: B100%, 1.5mL/min Eluent Acetonitrile / 50mM Ammonium acetate = 10/90 Detection UV254nm Flow Rate 1.0mL/min. Temperature 40°C Detection VIS550nm Sample Vol. 10μL Temperature 40°C Samples 1. L-Ascorbic acid (Vitamin C) 0.2mg/mL Sample Vol. 20μL

2. Tocopherol (Vitamin E) 0.2mg/mL Samples Cyanocobalamin(Vitamin B12)

F071 Separation of Carotenoids

Column Mightysil RP-18(L)GP 150-4.6mmØ 5μm Eluent Acetonitrile/ Methanol = 50/50 Flow rate 1.0mL/min. Detection UV460nm Temperature 40°C Sample Vol. 10μL Samples 1. Lutein 4mg/mL 2. β-Cryptoxanthin 2mg/mL 3. Lycopene 20mg/mL 4. β-Carotene 20mg/mL

08 FOOD F004 F059 Separation of β-Carotene in Broccoli Separation of β-Carotene

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Chloroform/ Methanol = 20/80 Column Mightysil RP-18GP 250-4.6mmØ 5μm Flow Rate 1.0mL/min. Eluent Ethanol 100% Detection 453nm 0.005AUFS Flow Rate 1.0mL/min. Temperature Room Temperature Detection UV-VIS 453nm Sample Vol. 10μL Temperature 40°C Sample 1. β-Carotene Sample Vol. 10μL Standard: 0.5μg/mL Actual: Extraction in acetone Samples 1. β-Carotene 2μg/mL

F007 Separation of Synthetic Antibiotics (Quinolone Derivatives) - 1

Column Mightysil RP-18 150-4.6mmØ 5μm

Eluent Acetonitrile/ 50mM Sodium dihydrogenphosphate buffer (pH2.5*) = 35/ 65, including 3.5mM of Sodium dodecyl sulfate

[*pH adjust : H3PO4]

Flow Rate 0.6mL/min. Detection UV320nm 0.04AUFS Temperature 25°C Sample Vol. 10μL (50ng) Samples 1. Oxolinic acid 2. Nalidixic acid 3. Flumequine 4. Ofloxacin 5. Benofloxacin 6. Danofloxacin 7. Piromidic acid 8. Enrofloxacin 9. Orbifloxacin Each 5μg/mL

FOOD 09 F008 Separation of Synthetic Antibiotics (Quinolone Derivatives) - 2

Column Mightysil RP-18GP 150-4.6mmØ 5μm

Eluent Acetonitrile/ 50mM Sodium dihydrogenphosphate buffer (pH 2.4*) including 2.5mM of Sodium heptanesulfonate = 20/ 80

[*pH adjust : conc.H3PO4]

Flow Rate 0.6mL/min. Detection Fluorescence 445nm ( Excitation 295nm) Temperature 40°C Sample Vol. 5μL Samples 1. Danofloxacin mesylate 2. Enrofloxacin

F009 Separation of Synthetic Antibiotics (Sulfa drugs)

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ 25mM Sodium dihydrogenphosphate= 25/75 Flow Rate 0.5mL/min. Detection UV265nm 0.04AUFS Temperature 40°C Sample Vol. 5μL Samples 1. Sulfisomidine 5μg/mL 2. Sulfathiazole 10μg/mL 3. Sulfamerazine 10μg/mL 4. Sulfamethoxypyridazine 10μg/mL 5. Sulfamonomethoxine 10μg/mL 6. Sulfachlorpyridazine 10μg/mL 7. Sulfamethoxazole 10μg/mL 8. Sulfadimethoxine 10μg/mL

10 FOOD F010 F011 Separation of Synthetic Antibiotics (Furan Derivatives) Separation of Synthetic Antibiotics (Protozoicides)

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ 25mM Sodium dihydrogenphosphate= 50/50 Column Mightysil RP-18 150-4.6mmØ 5μm Flow Rate 0.5mL/min. Eluent Acetonitrile/ 25mM Sodium dihydrogenphosphate= 35/65 Detection UV265nm 0.04AUFS Flow Rate 0.5mL/min. Temperature 40°C Detection UV265nm 0.04AUFS Sample Vol. 5μL Temperature 40°C Samples 1. Amprolium 15μg/mL Sample Vol. 5μL 2. Pyrimethamine 5μg/mL Samples 1. Nitro furazone 10μg/mL 3. Ethopabate 5μg/mL 2. Furazolidone 10μg/mL 4. Nicarbazin 65μg/mL

F012 Separation of Synthetic Antibiotics (Protozoicide & Quinolone)

Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent Acetonitrile/ 10mM Potassium dihydrogenphosphate including 5mM of Sodium heptanesulfonate = 20/80 Flow Rate 1.0mL/min. Detection UV265nm 0.04AUFS Temperature 40°C Sample Vol. 5μL Samples 1. Amprolium 13.75μg/mL 2. Oxolinic acid 3.75μg/mL

FOOD 11 F013 Separation of 18 Synthetic Antibiotics

Column Mightysil RP-18 250-4.6mmØ 5μm Eluent Acetonitrile/ 25mM Potassium dihydrogenphosphate = 15/ 85 (Eluent A), = 70/30 (Eluent B) Flow Rate 0.5mL/min. Detection UV265nm 0.04AUFS Temperature 40°C Sample Vol. 10μL Samples ODX: Olaquindox OMP: Ormethoprim OXA: Oxolinic acid 1μg/mL each SMR: Sulfamerazine SMM: Sulfamonomethoxine PYR: Pyrimethamine CLP: Clopidol CDX: Carbadox DFZ: Difurazone FZD: Furazolidone MRT: Morantel citrate NAA: Nalidixic acid TMP: Trimethoprim SDM: Sulfadimethoxine PMA: Piromidic acid SDD: Sulfadimidine SQX: Sulfaquinoxaline NCZ: Nicarbazin

Separation of 18 Synthetic Antibiotics

12 FOOD F026 F027 Separation of Carbadox Separation of a Metabolite of Carbadox

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent Acetonitrile/ 10mM Potassium dihydrogenphosphate buffer

Eluent Acetonitrile/ 25mM Potassium dihydrogenphosphate= 1/9 (pH2.5*)= 1/ 4 [*pH adjust : conc.H3PO4] Flow Rate 1.0mL/min. Flow Rate 0.3mL/min. Detection UV310nm Detection UV320nm Temperature 40°C Temperature 40°C Sample Vol. 2μL Sample Vol. 5μL Sample 1. Carbadox 10μg/mL Sample 1. 2-Quinoxalinecarboxylic acid 50μg/mL

F028 F006 Separation of Sulfadimidine Separation of Tetracycline Antibiotics

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ 50mM Phosphate buffer(pH2.5) = 25/75 Flow Rate 1.0mL/min. Column Mightysil RP-18GP 150-4.6mmØ 5μm Detection UV350nm 0.04AUFS Eluent Acetonitrile/ 25mM Sodium dihydrogenphosphate = 3/17 Temperature 25°C Flow Rate 1.0mL/min. Sample Vol. 10μL (25ng) Detection UV268nm Samples 1. Oxytetracycline Temperature 40°C 2. Tetracycline Sample Vol. 20μL 3. Chlortetracycline Sample 1. Sulfadimidine 0.5μg/mL 4. Doxycycline

FOOD 13 F014 Separation of Drugs for Animals – 1

Column Mightysil RP-18 150-4.6mmØ 5μm

Eluent A: Methanol/ 25mM of potassium dihydrogenphosphate = 20/80 B: Methanol/ 25mM of potassium dihydrogenphosphate = 95/5 Linear gradient from 100% of A to 100% of B for 25 min.

Flow Rate 1.0mL/min. Detection UV254nm Temperature 40°C Sample Vol. 20μL Samples 1. Oxytetracycline 50μg/mL 2. Flubendazole 5μg/mL 3. β-Trenbolone 20μg/mL 4. α-Trenbolone 20μg/mL 5. Zeranol 20μg/mL 6. Closantel 10μg/mL 7. Ivermectin 20μg/mL

F015 F019 Separation of Drugs for Animals – 2 Analysis of Oxytetracycline in Pork

Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent Acetonitrile/ Phosphate buffer pH3.0 = 15/85 Flow Rate 0.5mL/min. Detection UV285nm 0.03AUFS Column Mightysil RP-18 150-6.0mmØ 5μm Temperature 40°C Eluent 1M of Imidazole buffer(pH7.2)/ Methanol = 4/1 Sample Vol. 5μL Flow Rate 1.0mL/min. Samples 1. 5-Hydroxythiabendazole 5μg/mL Detection Fluorescence 520nm (Excitation 380nm) 2. 5-Propylsulfonyl-1H-benzimidazole-2-amine 5μg/mL Temperature 40°C 3. 2-Quinoxalinecarboxylic acid 15μg/mL Sample Vol. 20μL 4. Sulfadimidine 7μg/mL Sample 0.1ppm of Oxytetracycline was added to pork

14 FOOD F016 F017 Analysis of α-Trenbolone in Pork Analysis of β-Trenbolone in Pork

Column Mightysil RP-18 150-4.6mmØ 5μm Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ Water = 5/6 Eluent Acetonitrile/ Water = 5/6 Flow Rate 0.5mL/min. Flow Rate 0.5mL/min. Detection UV340nm Detection UV340nm Temperature 40°C Temperature 40°C Sample Vol. 100μL Sample Vol. 100μL Sample 0.01ppm of α-Trenbolone was added to pork Sample 0.02ppm of β-Trenbolone was added to pork

F021 F018 Analysis of Zeranol in Pork Analysis of Ivermectin in Pork

Column Mightysil RP-18 150-4.6mmØ 5mm Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ Water = 5/6 Eluent Methanol/ Water = 95/5 Flow Rate 0.5mL/min. Flow Rate 1.5mL/min. Detection UV230nm Detection Fluorescence 425nm (Excitation 380nm) Temperature 40°C Temperature 40°C Sample Vol. 100μL Sample Vol. 20μL Sample Actual pork (muscles) Sample 0.02ppm of Ivermectin was added to pork

FOOD 15 F020 F022 Analysis of Closantel in Pork Analysis of Flubendazole in Pork

Column Mightysil RP-18 150-6.0mmØ 5μm Eluent Methanol/ 20mM Sodium dihydrogenphosphate buffer(pH3.3*) = Column Mightysil RP-18 150-4.6mmØ 5μm

7/ 2 [*pH adjust : H3PO4] Eluent Acetonitrile/ Water = 7/13 Flow Rate 1.5mL/min. Flow Rate 1.0mL/min. Detection UV369nm Detection UV313nm Temperature 50°C Temperature 40°C Sample Vol. 20μL Sample Vol. 20μL Sample 1ppm of Closantel was added to pork Sample 0.01ppm of Flubendazole was added to pork

F029 F030 Separation of Thiabendazole Separation of a Metabolite of Thiabendazole

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent Acetonitrile/ 25mM Sodium dihydrogenphosphate = 1/9 Eluent Acetonitrile/ 25mM Sodium dihydrogenphosphate = 1/9 Flow Rate 1.5mL/min. Flow Rate 0.5mL/min. Detection Fluorescence 370nm (Excitation 300nm) Detection UV298nm Temperature 40°C Temperature 40°C Sample Vol. 1μL Sample Vol. 100μL Sample 1. Thiabendazole 0.25μg/mL Sample 1. 5-Hydroxythiabendazole 0.05μg/mL

16 FOOD F023 F024 Separation of Albendazole Separation of a Metabolite of Albendazole

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent Acetonitrile/ 25mM Sodium dihydrogenphosphate = 4/6 Eluent Acetonitrile/ 25mM Sodium dihydrogenphosphate = 3/17 Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV220nm 0.35AUFS Detection Fluorescence 320nm (Excitation 280nm) 0.14AUFS Temperature 40°C Temperature 40°C Sample Vol. 20μL Sample Vol. 1μL Sample 1. Albendazole 0.01mg/mL Sample 1. 5-Propylsulfonyl-1H-benzimidazole-2-amine 0.5μg/mL

F025 F031 Separation of Isomethamidium Separation of Avoparcins

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18GP 250-4.6mmØ 5μm Eluent Acetonitrile/ 30mM Citric acid including 5mM of Sodium Eluent Acetonitrile/ 2.5% Acetate buffer(pH4.0*) including 10mM of

heptanesulfonic acid = 3/7 Sodium heptanesulfonate = 11.5/ 88.5 [*pH adjust : NH4OH] Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV380nm Detection UV280nm Temperature 40°C Temperature Room Temperature (22°C) Sample Vol. 15μL Sample Vol. 20μL Sample 1. Isometamidium 0.1μg/mL Sample Control material including α-Avoparcin and β-Avoparcin

FOOD 17 F044 F047 Separation of Preservatives - 1 Separation of Presevatives -2

Column Mightysil RP-18 150-4.6mmØ 5μm Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ 20mM Phosphate buffer(pH4.6) = 1/2 Eluent Acetonitrile/ 20mM Buffers pH4.6 = 1/2 Flow Rate 0.5mL/min. Flow Rate 0.5mL/min. Detection UV225nm 1.28AUFS Detection UV225nm 0.32AUFS Temperature Room Temperature Temperature Room Temperature Sample Vol. 20μL Sample Vol. 20μL Samples 1. Benzoic acid 20μg/mL Samples 1. Benzoic acid 20μg/mL 2. Sorbic acid 20μg/mL 2. Sorbic acid 20μg/mL 3. Dehydro acetic acid 20μg/mL 3. Dehydroacetic acid 20μg/mL

F075 F043 Separation of Presevatives - 3 Separation of Preservatives - 4

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18GP 150-4.6mmØ 5μm

Eluent 5mM Citric acid buffer*/ Acetonitrile/ Methanol = 70/ 20/10 Eluent Methanol/ Water = 1/1 [*0.7g/L Citric acid monohydrate + 0.6g/L Trisodium citrate Flow Rate 1.0mL/min. dehydrate] Detection UV254nm Flow Rate 1.0mL/min. Temperature 40°C Detection UV230nm Sample Vol. 10μL Temperature 40°C Samples 1. Methyl p-hydroxybenzoate 1μg/mL Sample Vol. 10μL 2. Ethyl p-hydroxybenzoate 1μg/mL Samples 1. Benzoic acid 100μg/mL 3. Isopropyl p-hydroxybenzoate 1μg/mL 2. Sorbic acid 100μg/mL 4. n-Propyl p-hydroxybenzoate 1μg/mL 3. Salicylic acid 100μg/mL 5. Isobutyl p-hydroxybenzoate 1μg/mL 4. Dehydroacetic acid 100μg/mL 6. n-Butyl p-hydroxybenzoate 1μg/mL

18 FOOD F074 Separation of Preservatives - 5

Column Mightysil RP-18GP 150-4.6mmØ 5μm

Eluent Methanol/ 5mM Citric acid buffer* = 50/50 [*0.7g/L Citric acid monohydrate + 0.6g/L Trisodium citrate dihydrate]

Flow Rate 1.0mL/min. Detection UV270nm Temperature 40°C Sample Vol. 20μL Samples 1. Methyl p-hydroxybenzoate 4.0μg/mL 2. Ethyl p-hydroxybenzoate 4.0μg/mL 3. Isopropyl p-hydroxybenzoate 4.0μg/mL 4. Propyl p-hydroxybenzoate 4.0μg/mL 5. Isobutyl p-hydroxybenzoate 4.0μg/mL 6. Butyl p-hydroxybenzoate 4.0μg/mL

F077 Separation of Presevatives - 6

Column Mightysil RP-8GP 150-4.6mmØ 5μm

Eluent Ethanol/ 50mM Sodium dihydrogen phosphate dihydrate = 34/ 66, including 2mM of Cetyltrimethylammonium bromide (pH4.41*) [*pH adjust : 10% Phosphoric acid, after mixing the components of eluent.]

Flow Rate 1.0mL/min. Detection UV240nm 0.32AUFS Temperature 45°C Sample Vol. 5μL Samples 1. Ethyl p-hydroxybenzoate 0.1mg/mL 2. Sorbic acid 0.1mg/mL 3. Benzoic acid 0.1mg/mL 4. Isopropyl p-hydroxybenzoate 0.1mg/mL 5. Propyl p-hydroxybenzoate 0.1mg/mL 6. Saccharin 0.1mg/mL 7. Isobutyl p-hydroxybenzoate 0.1mg/mL 8. Butyl p-hydroxybenzoate 0.1mg/mL

FOOD 19 F061 F042 Separation of Preservatives and Saccharin Separation of Antioxidants

Column Mightysil RP-18GP 150-4.6mmØ 5μm

Eluent 0.1% Pottasium phosphate buffer*/ Acetonitrile = 95/5 [*0.1% Pottasium dihydrogen phosphate / 0.1% Dipotassium Column Mightysil RP-18 150-4.6mmØ 5μm hydrogen phosphate = 1/1] Eluent Methanol/ Water = 8/2 Flow Rate 0.7mL/min. Flow Rate 1.0mL/min. Detection UV225nm Detection UV280nm 0.16AUFS Temperature 40°C Temperature Room Temperature Sample Vol. 10μL Sample Vol. 10μL Samples 1. Benzoic acid 10μg/mL Samples 1. Butylhydroquinone (BHQ) 100μg/mL 2. Sorbic acid 10μg/mL 2. Butylhydroxyanisole (BHA) 100μg/mL 3. Saccharin 10μg/mL 3. Butyl hydroxytoluene (BHT) 100μg/mL

F073 Separation of Antioxdants - 2

Column Mightysil RP-18GP 150-4.6mmØ 5μm

Eluent A: Acetonitrile/ Methanol = 50/50 B: 0.5% Acetic acid Gradient (A%) :0~25min. : A45%~100% Linear, 25.1~30min.: A 100%, 30.1~50min.: A45%

Flow Rate 1.0mL/min. Detection UV280nm Temperature 40°C Sample Vol. 10μL Samples 1. Propyl gallate (PG) 40μg/mL 2. Trihydroxybutyrophenone (THBP) 40μg/mL 3. Butyl hydroquinone (BHQ) 40μg/mL 4. Nordihydroguaiaretic acid (NDGA) 40μg/mL 5. Butyl hydroxyanisole (BHA) 40μg/mL 6. Hydroxy methyl butyl phenol (HMBP) 40μg/mL 7. Octyl gallate (OG) 40μg/mL 8. Butyl hydroxytoluene (BHT) 40μg/mL 9. Dodecyl gallate (DG) 40μg/mL

20 FOOD F048 F045 Separation of Ascorbic Acid & Erythorbic Acid Separation of anti-Fungal Preservatives - 1

Column Mightysil RP-18 150-4.6mmØ 5μm Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Methanol/ Water = 75/25 Eluent 50mM Disodium hydrogen phosphate buffer(pH6.8*) including Flow Rate 1.0mL/min. 5mM of Tetrabutylammonium hydrogensulfate Detection UV230nm 0.16AUFS Flow Rate 1.0mL/min. Temperature 40°C Detection UV254nm Sample Vol. 3μL Temperature 40°C Samples 1. Thiabendazole (TBZ) 160μg/mL Sample Vol. 1μL 2. o-Phenylphenol (OPP) 40μg/mL Samples 1. Ascorbic acid 1mg/mL 3. Imazalil (IMZ) 70μg/mL 2. Erythorbic acid 1mg/mL 4. Biphenyl (BP) 50μg/mL

F046 F037 Separation of anti-Fungal Preservatives - 2 Separation of Food Dyes - 1

Column Mightysil RP-18GP 150-4.6mmØ 5μm

Eluent Acetonitrile/ 0.05% Potassium phosphate buffer* = 6/4 Column Mightysil RP-18 150-4.6mmØ 5μm [*0.05% Potassium dihydrogen phosphate/0.05% Dipotassium Eluent A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9 hydrogen phosphate = 1/1] B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2 Flow Rate 0.65mL/min. Linear gradient from 100% of A to 100% of B for 25min. Detection Fluorescence 325nm (Excitation 280nm) Flow Rate 1.0mL/min. Temperature 40°C Detection UV254nm 0.16AUFS Sample Vol. 5μL Temperature Room Temperature Samples 1. Thiabendazole (TBZ) 1μg/mL Sample Vol. 10μL 2. o-Phenylphenol (OPP) 1μg/mL Samples 1. Tartrazine (Yellow-4) 40μg/mL 3. Biphenyl (BP) 1μg/mL 2. Sunset Yellow FCF (Yellow-5) 60μg/mL

FOOD 21 F038 Separation of Food Dyes - 2

Column Mightysil RP-18 150-4.6mmØ 5μm

Eluent A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9 B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2 Linear gradient from 100% of A to 100% of B for 25min.

Flow Rate 1.0mL/min. Detection UV254nm 0.16AUFS (A), UV612nm 0.64AUFS (B) Temperature Room Temperature Sample Vol. 20μL Samples 1. Indigocarmine (Blue-2) 40μg/mL 2. Fast Green FCF (Green-3) 30μg/mL 3. Brilliant Blue FCF (Blue-1) 30μg/mL

F039 Separation of Food Dyes - 3

Column Mightysil RP-18 150-4.6mmØ 5μm

Eluent A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9 B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2 Linear gradient from 100% of A to 100% of B for 25min., then keeping 100% of B.

Flow Rate 1.0mL/min. Detection UV254nm 0.16AUFS Temperature Room Temperature Sample Vol. 10μL Samples 1. Amaranth (R2) 40μg/mL 2. New Coccine (R102) 40μg/mL 3. Acid Red (R106) 40μg/mL 4. Eosin Y 40μg/mL 5. Erythrosine (R3) 40μg/mL 6. Phloxine (R104) 40μg/mL 7. Rose Bengal (R105) 60μg/mL

22 FOOD F040 Separation of Food Dyes - 4

Column Mightysil RP-18 150-4.6mmØ 5μm

Eluent A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9 B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2 Linear gradient from 100% of A to 100% of B for 25min., then keeping 100% of B.

Flow Rate 1.0mL/min. Detection UV254nm 0.16AUFS Temperature Room Temperature Sample Vol. 20μL Samples 1. Tartrazine (Y4) 20μg/mL 2. Amaranth (R2) 20μg/mL 3. Indigocarmine (B2) 30μg/mL 4. New Coccine (R102) 20μg/mL 5. Sunset Yellow FCF (Y5) 30μg/mL 6. Fast Green FCF (G3) 50μg/mL 7. Brilliant Blue FCF (B1) 50μg/mL 8. Acid Red (R106) 20μg/mL 9. Eosin Y 20μg/mL 10. Erythrosine (R3) 20μg/mL 11. Phloxine (R104) 50μg/mL 12. Rose Bengal (R105) 30μg/mL

F041 Separation of Food Dyes - 5

Column Mightysil RP-18 150-4.6mmØ 5μm

Eluent A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9 B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2 Linear gradient from 100% of A to 100% of B for 25min.

Flow Rate 1.0mL/min. Detection UV428nm (0-10min.); UV630nm (10-20min.); UV254nm (20-30min.) 0.08AUFS Temperature Room Temperature Sample Vol. 20μL Samples 1. Tartrazine Aluminium Lake 50μg/mL 2. Brilliant Blue Aluminium Lake 50μg/mL 3. Erythrosine Aluminium Lake 50μg/mL

FOOD 23 F072 Separation of Food Dyes - 6

Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent A: Methanol/ Acetonitrile/ Water = 3/3/4 Containing Ammonium acetate solution(5g/100mL) B: Ammonium acetate solution(5g/100mL) Gradient : 0~30min. : A5%-A100% Linear, 30~36min.: A100%, 36.1~50min.: A5% Flow Rate 1.4mL/min. Detection UV254nm Temperature 40°C Sample Vol. 20μL Samples 1. Tartrazine (Y4) 2. Amaranth (R2) 3. Indigocarmine (B2) 4. New Coccine (R102) 5. Sunset Yellow FCF (Y5) 6. Allura Red AC (R40) 7. Fast Green FCF (G3) 8. Brilliant Blue FCF (B1) 9. Eosin 10. Erythrosine (R3) 11. Acid Red (R106) 12. Phloxine (R104) 13. Rose Bengal (R105) (Each Sample: 20μg/mL)

F060 F050 Separation of Anthocyanins Separation of Aspartame in a Beverage

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Methanol/ 33mM Phosphate buffer(pH4.9*) = 3/7

Column Mightysil RP-18GP 250-4.6mmØ 5μm [*pH adjust : H3PO4] Eluent A : 0.5% Trifluoroacetic acid Flow Rate 1.0mL/min. B : Trifluoroacetic acid/ Acetonitrile = 50/50 Detection UV210nm 0.16AUFS Flow Rate 1.0mL/min. Temperature Room Temperature Detection UV-VIS 520nm Sample Vol. 2μL Temperature 40°C Sample Actual beverage: Sample Vol. 10μL 1. Benzoic acid Samples 1. Acylanthocyanin 1mg/mL 2. Caffeine 2. Anthocyanin 3mg/mL 3. Aspartame

24 FOOD F069 F080 Separation of Acesulfame K Separation of Aspartame and Acesulfame K in breath mint

Column Mightysil RP-18 GP 150-4.6mmØ 5μm

Eluent Methanol/ 10mM Potassium dihydrogenphosphate(pH4.0*) including 10mM of Tetra-n-propylammonium hydroxide = 25/75

[*pH adjust : H3PO4]

Flow Rate 1.0mL/min. Column Mightysil RP-18GP 150-4.6mmØ 5μm Detection DAD (200 〜 400nm) Eluent Acetonitrile/ 10mM Tetrabutylammonium Temperature 40°C dihydrogenphosphate = 40/60 Sample Vol. 10μL Flow Rate 1.0mL/min. Samples 1. Aspartame Detection UV227nm 2. Acesulfame K Temperature 40°C Each 0.1mg/mL in Water Sample Vol. 20μL Commercial breath mint: Samples 1. Acesulfame K 0.01mg/mL 5 tablets of breath mint dissolved to 10mL of Water and filtered 2. Ethyl-p-hydroxybenzoate 0.01mg/mL with 0.2μm PVDF membrane.

F049 F076 Separation of Saccharin & Sorbic Acid Separation of Cyclamic acid

Column Mightysil RP-18GP 250-4.6mmØ 5μm

Eluent Acetonitrile/ 0.1% Potassium phosphate buffer* = 5/95 [*0.1% Potassium dihydrogen phosphate/ 0.1% Dipotassium hydrogen phosphate = 1/1 ] Column Mightysil RP-18GP 150-4.6mmØ 5μm Flow Rate 0.8mL/min. Eluent Methanol/ Water = 80/20 Detection UV230nm Flow Rate 1.0mL/min. Temperature 40°C Detection UV314nm Sample Vol. 10μL Temperature 40°C Samples 1. Saccharin 10μg/mL Sample Vol. 20μL 2. Sorbic acid 10μg/mL Samples 1. Cyclamic acid 20μg/mL

FOOD 25 F051 F089 Separation of Pyrazines Separation of Ubiquinone 9, 10

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ 10mM Potassium dihydrogenphosphate = 3/7 Flow Rate 0.15mL/min. Column Mightysil RP-18GP 150-4.6mmØ 5μm Detection UV273nm 0.32AUFS Eluent Methanol/ Ethanol = 13/7 Temperature Room Temperature Flow Rate 2.0mL/min. Sample Vol. 5μL Detection UV275nm Samples 1. Pyrazine Temperature 35°C 2. 2-Methylpyrazine Sample Vol. 5μL 3. 2,5-Dimethylpyrazine Samples 1. Ubiquinone 9 (Coenzyme Q9) 4. 2,3,5-Trimethylpyrazine 2. Ubiquinone 10 (Coenzyme Q10) 5. 2,3,5,6-Tetramethylpyrazine Total of 500ppm

F090 F091 Separation of DL-Thioctic acid Separation of Benzoyl Peroxide in Flour

Column Mightysil RP-18GP 250-4.6mmØ 5μm Eluent Methanol/ Acetonitrile/ 0.005mol/L Phosphate Buffer

(pH3.08*)=1160/180/920[*pH adjust : dil.H3PO4] Column Mightysil RP-18GP 250-4.6mmØ 5μm Flow Rate 1.2mL/min. Eluent Acetonitrile / Water = 55/45 Detection UV215nm Flow Rate 1.0mL/min. Temperature 35°C Detection UV235nm Sample Vol. 20μL Temperature 40°C Samples DL-Thioctic acid 1mg/mL Sample Vol. 20μL ( 0.005mol/L Phosphate buffer pH3.5/Acetonitrile=1/1.) Preparation The Acetonitrile is added to flour , and filtered

26 FOOD F053 Separation of Caffeine and Catechins

Column Mightysil RP-18GP 150-4.6mmØ 5μm

Eluent A: 0.1% Phosphoric acid including 5% of N,N-Dimethylformamide and 0.1% of Acetonitrile B: Acetonitrile

Flow Rate 1.0mL/min. Detection UV280nm 0.08AUFS Temperature 43°C Sample Vol. 5μL (Standards), 3μL (Actual sample): Extracted with mixture of Acetonitrile and Water Samples 1. Caffeine 0.10mg/mL in STD. 2. Epigallocatechin 0.05mg/mL in STD. 3. Catechin 0.05mg/mL in STD 4. Epicatechin 0.05mg/mL in STD. 5. Epigallocatechin Gallate 0.05mg/mL in STD 6. Epicatechin Gallate 0.05mg/mL in STD.

F054 Separation of Commercial Tea drink

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent A : Acetonitrile/ Water/ Phosphoric acid = 10/ 400/ 0.2 B : Methanol/ Acetonitrile/ Water/ Phosphoric acid = 200/ 10/ 400/ 0.2 A 100% : 0~3min., A100%~A0%(B100%) : 3~25min., B 100% : 25~26min.

Flow Rate 1.0mL/min. Detection UV230nm Temperature 40°C Sample Vol. 10μL (Commercial green tea drink) Samples 1. Gallic acid 2. Theogallin 3. Catechin gallate 4. Epigallocatechin 5. Catechin 6. Caffeine 7. Epigallocatechin gallate 8. Epicatechin 9. Gallocatechin gallate 10. Epicatechin gallate 11. Catechin gallate

FOOD 27 F055 Separation of Catechins

Column Mightysil RP-18 150-4.6mmØ 5μm

Eluent A: 0.1% Phosphoric acid containing 0.1% Acetonitrile and 5% N,N- Dimethylformamide B : Acetonitrile

Flow Rate 1.0mL/min. Detection UV280nm Temperature 40°C Sample Vol. 5μL Samples 1. Caffeine 2. Epigallocatechin 3. Catechin 4. Epicatechin 5. Epigallocatechin gallate 6. Gallocatechin gallate 7. Epicatechin gallate 8. Catechin gallate

F056 Separation of Catechins, Caffeine, Chlorophyll A

Column Mightysil RP-18GP 150-4.6mmØ 5μm

Eluent A: 1% Phosphoric acid, B : Ethanol Time (min.) 0 0.1 17 20 35 A 95 90 80 5 5 B 5 10 20 95 95

Flow Rate 1.0mL/min. Detection 0~21min. : UV280nm, 21~35min.: UV430nm Temperature 40°C Sample Vol. 5μL Samples 1. Epigallocatechin 20μg/mL 2. Catechin 20μg/mL 3. Caffeine 4μg/mL 4. Epicatechin 20μg/mL 5. Epigallocatechin gallate 10μg/mL 6. Gallocatechin gallate 6μg/mL 7. Epicatechin gallate 8μg/mL 8. Catechin gallate 6μg/mL 9. Chlorophyll a 6μg/mL

28 FOOD F063 F064 Separation of Allyl isothiocyanate Separation of Quassins

Column Mightysil RP-8GP 250-4.6mmØ 5μm Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent Acetonitrile/ Water = 70/30 Eluent Acetonitrile/ Water = 35/75 Flow Rate 0.8 mL/min. Flow Rate 1.0mL/min. Detection UV240nm Detection UV254nm Temperature 40°C Temperature 30°C Sample Vol. 10μL Sample Vol. 10μL Samples 1. Allyl isothiocyanate 0.4mg/mL Samples 1.2. : Quassin 100μg/mL 2. Fenethylcyanate 0.3mg/mL 3. p-Methylacetophenon 12.5μg/mL

F065 F082 Separation of Capsaicins Separation of Curcumin

Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent Acetonitrile/20mM Formic acid = 45/55 Column Mightysil RP-18GP 150-4.6mmØ 5μm Flow rate 1.0mL/min. Eluent Acetonitrile/ Water/ Acetic acid = 43/ 57/ 1 Detection UV425nm Flow Rate 1.2 mL/min. Temperature 40°C Detection UV280nm Sample Vol. 5μL Temperature 45°C Samples Curcumin 0.1mg/mL Sample Vol. 15μL Commercial drink Samples 1. Capsaicin 160μg/mL The sample solution filtered with PVDF filter, after having diluted 2. Dihydrocapsaicin 80μg/mL the commercial drink to 3 times with Acetonitrile.

FOOD 29 F083 F078 Separation of Curcumin in a commercial drink by LC/MS Separation of Rutin and Quercetin

Column Mightysil RP-18GP 150-2.0mmØ 5μm Eluent Acetonitrile/20mM Formic acid = 45/55 Column Mightysil RP-18GP 250-4.6mmØ 5μm Flow Rate 0.2mL/min. Eluent 2.5% Acetic acid/ Methanol/ Acetonitorile = 70/ 10/ 20 Detection MS ESI- Flow Rate 1.0mL/min. Temperature 40°C Detection UV350nm Sample Vol. 5μL Temperature 30°C Samples Commercial drink Sample Vol. 10μL The sample solution filtered with PVDF filter, after having diluted Samples 1. Rutin 0.8mg/mL the commercial drink to 3 times with Acetonitrile. 2. Quercetin 0.4mg/mL

F099 F100 Separation of Apigenin Separation of 5,6-Benzoflavone

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*) Eluent Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*)

= 30/70 [*pH adjust : H3PO4] = 60/40 [*pH adjust : H3PO4] Flow rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV340nm Detection UV270nm Temperature 40ºC Temperature 40ºC Sample Vol. 50μL Sample Vol. 10μL Samples 1. Apigenin 0.02mg/mL Sample 1. 5,6-Benzoflavone 0.2mg/mL

30 FOOD F101 F102 Separation of 7,8-Benzoflavone Separation of Biochanin A

Columns Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*) Eluent Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*)

= 55/45 [*pH adjust : H3PO4] = 50/50 [*pH adjust : H3PO4] Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV280nm Detection UV260nm Temperature 40ºC Temperature 40ºC Sample Vol. 10μL Sample Vol. 5μL Sample 1. 7,8-Benzoflavone 0.2mg/mL Sample 1. Biochanin A 0.5mg/mL

F103 F104 Separation of Chrysin Separation of Diosmin

= 40/60 [*pH adjust : H3PO4] = 20/80 [*pH adjust : H3PO4] Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV265nm Detection UV254nm Temperature 40ºC Temperature 40ºC Sample Vol. 5μL Sample Vol. 5μL Sample 1. Chrysin 0.1mg/mL Sample 1. Diosmin 0.1mg/mL

FOOD 31 F105 F106 Separation of Flavonol Separation of Hesperetin

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH7.0*) Eluent Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH7.0*)

= 50/50 [*pH adjust : H3PO4] = 30/70 [*pH adjust : H3PO4] Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV240nm Detection UV285nm Temperature 40ºC Temperature 40ºC Sample Vol. 10μL Sample Vol. 5μL Sample 1. Flavonol 0.2mg/mL Sample 1. Hesperetin 0.5mg/mL

F107 F108 Separation of Hesperidin Separation of Isoquercitrin

= 20/80 [*pH adjust : H3PO4] = 20/80 [*pH adjust : H3PO4] Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV285nm Detection UV254nm Temperature 40ºC Temperature 40ºC Sample Vol. 10μL Sample Vol. 5μL Sample 1. Hesperidin 0.1mg/mL Sample 1. Isoquercitrin 0.5mg/mL

32 FOOD F109 F110 Separation of Kaempferol Separation of Luteolin

= 30/70 [*pH adjust : H3PO4] = 25/75 [*pH adjust : H3PO4] Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV360nm Detection UV345nm Temperature 40ºC Temperature 40ºC Sample Vol. 20μL Sample Vol. 20μL Sample 1.Kaempferol 0.2mg/mL Sample 1.Luteolin 0.1mg/mL

F111 F112 Separation of Naringenin Separation of Naringin

= 30/70 [*pH adjust : H3PO4] = 20/80 [*pH adjust : H3PO4] Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV290nm Detection UV280nm Temperature 40ºC Temperature 40ºC Sample Vol. 20μL Sample Vol. 10μL Sample 1.Naringenin 0.2mg/mL Sample 1. Naringin 0.05mg/mL

FOOD 33 F113 F114 Separation of Rhoifolin Separation of Trihydroxychalcone

= 20/80 [*pH adjust : H3PO4] = 35/65 [*pH adjust : H3PO4] Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV330nm Detection UV370nm Temperature 40ºC Temperature 40ºC Sample Vol. 5μL Sample Vol. 10μL Sample 1. Rhoifolin 0.03mg/mL Sample 1. 4,2,4-Trihydroxychalcone 0.5mg/mL

F066 F068 Separation of Oligosaccharide Separation of Carbohydrates

Column Mightysil RP-18GP Aqua 250-4.6mmØ 5μm Eluent 100% Water Flow rate 0.25mL/min. Detection RI Temperature 35°C Column Mightysil RP-18GP Aqua 250-4.6mmØ 5μm Sample Vol. 5μL Eluent 100% Water Samples 1. Glucose Flow rate 0.25mL/min. 2. Maltose Detection RI 3. Maltotriose Temperature 35°C 4. Maltotetraose Sample Vol. 5μL 5. Maltopentaose Samples 1. Glucose 1.0mg/mL 6. Maltohexaose 2. Sucrose 1.0mg/mL 7. Maltoheptaose Each 1.0mg/mL 3. Raffinose 1.0mg/mL

34 FOOD F032 F092 Separation of di-Carbonic Acids-1 Separation of di-Carbonic Acids-2

Column Mightysil RP-18 150-4.6mmØ 5μm Column Mightysil RP-18GP Aqua 250-4.6mmØ 5μm Eluent Acetonitrile/ 20mM Phosphoric acid = 10/90 Eluent Acetonitrile/20mM Phosphoric acid = 10/90 Flow Rate 1.0mL/min. Flow Rate 1.0mL / min. Detection UV210nm 0.04AUFS Detection UV210nm 0.04AUFS Temperature Room Temperature Temperature Room Temperature Sample Vol. 20μL Sample Vol. 20μL Samples 1. Malonic acid 150μg/mL Samples 1. Malonic acid 150μg/mL 2. Succinic acid 200μg/mL 2. Succinic acid 200μg/mL 3. Glutaric acid 200μg/mL 3. Glutaric acid 200μg/mL 4. Adipic acid 200μg/mL 4. Adipic acid 200μg/mL 5. Pimelic acid 250μg/mL 5. Pimelic acid 250μg/mL

F035 F093 Separation of Organic Acids - 1 Separation of Organic Acids – 2

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent 50mM Ammonium dihydrogenphosphate buffer (pH2.0*) Column Mightysil RP-18GP Aqua 250-4.6mmØ 5μm

[*pH adjust : conc.H3PO4] Eluent Ammonium dihydrogenphosphate buffer(pH2.0) Flow Rate 1.0mL/min. Flow Rate 1.0mL / min. Detection UV200nm 0.08AUFS Detection UV200nm 0.08AUFS Temperature Room Temperature Temperature Room Temperature Sample Vol. 5μL Sample Vol. 5μL Samples 1. Oxalic acid 0.1mg/mL Samples 1. Oxalic acid 0.1mg/mL 2. Glycolic acid 2.0mg/mL 2. Glycolic acid 2.0mg/mL 3. Malic acid 2.0mg/mL 3. Malic acid 2.0mg/mL 4. Lactic acid 3.0mg/mL 4. Lactic acid 3.0mg/mL 5. Citric acid 2.0mg/mL 5. Citric acid 2.0mg/mL

FOOD 35 F036 F094 Separation of Organic Acids - 3 Separation of Organic Acids - 4

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent 50mM Ammonium dihydrogenphosphate buffer(pH2.0*) Column Mightysil RP-18GP Aqua 250-4.6mmØ 5μm

[*pH adjust : conc.H3PO4] Eluent Ammonium dihydrogenphosphate buffer(pH2.0) Flow Rate 1.0mL/min. Flow Rate 1.0mL / min. Detection UV200nm 0.08AUFS Detection UV200nm 0.08AUFS Temperature Room Temperature Temperature Room Temperature Sample Vol. 5μL Sample Vol. 5μL Samples 1. Oxalic acid 20μg/mL 5. Maleic acid 75μg/mL Samples 1. Oxalic acid 20μg/mL 5. Maleic acid 75μg/mL 2. Tartaric acid 300μg/mL 6. Citric acid 400μg/mL 2. Tartaric acid 300μg/mL 6. Citric acid 400μg/mL 3. Malic acid 350μg/mL 7. Fumaric acid 75μg/mL 3. Malic acid 350μg/mL 7. Fumaric acid 75μg/mL 4. Malonic acid 500μg/mL 8. Acrylic acid 75μg/mL 4. Malonic acid 500μg/mL 8. Acrylic acid 75μg/mL

F098 F062 Separation of Organic Acids -5 Separation of Formic acid and Tartaric acid

Column Mightysil RP-18GP Aqua 150-4.6mmØ 3μm Eluent 20mM Ammonium dihydrogenphosphate buffer(pH2.48) Flow Rate 1.0mL/min. Column Mightysil RP-18GP 250-4.6mmØ 5μm Detection UV210nm Eluent 25mM Ammonium phosphate buffer (pH6.8) including 5mM of Temperature 35°C Tetrabutylammonium hydrogen sulphate Sample Vol. 20μL Flow Rate 1.0mL/min. Samples 1. Glycolic acid 100μg/mL 6. Maleic acid 1μg/mL Detection UV210nm 0.08AUFS 2. Malic acid 100μg/mL 7. Citric acid 100μg/mL Temperature 40°C 3. Malonic acid 100μg/mL 8. Fumaric acid 0.7μg/mL Sample Vol. 10μL 4. Lactic acid 100μg/mL 9. Succinic acid 100μg/mL Samples 1. Formic acid 0.7mg/mL 5. Acetic acid 100μg/mL 10.Acrylic acid 1μg/mL 2. Tartaric acid 0.3mg/mL

36 FOOD F033 F034 Separation of Oxalic Acid and Nitric Acid in Spinach Separation of Aromatic Acids-1

Column Mightysil RP-18 150-4.6mmØ 5μm Column Mightysil RP-18 150-4.6mmØ 5μm

Eluent 5mM Tetrabutylammonium hydrogen sulfate in 50mM of Eluent Acetonitrile/ 20mM phosphoric acid = 2/ 8 Diammonium hydrogenphosphate buffer(pH6.8*) Flow Rate 1.0mL/min.

[*pH adjust : H3PO4] Detection UV254nm 0.32AUFS Flow Rate 1.0mL/min. Temperature Room Temperature Detection UV210nm 0.04AUFS Sample Vol. 10μL Temperature Room Temperature Samples 1. Gallic acid 40μg/mL Sample Vol. 20μL 2. Protocatechuic acid 20μg/mL

Sample Actual spinach, extracted 5g of spinach solution was diluted with 3. Phthalic acid 100μg/mL 250mL of water and filtered 4. Gentisic acid 100μg/mL (1.Oxalic acid 2.Nitric acid ) 5. Salicylic acid 200μg/mL

F096 F095 Separation of Aromatic Acids -2 Separation of Aromatic Acids -3

Column Mightysil RP-18GP Aqua 250-4.6mmØ 5μm Column Mightysil RP-18GP Aqua 250-4.6mmØ 5μm Eluent Acetonitrile/ 20mM phosphoric acid = 2/8 Eluent Acetonitrile/ 20mM phosphoric acid = 1.5/8.5 Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV254nm 0.32AUFS Detection UV254nm 0.32AUFS Temperature Room Temperature Temperature Room Temperature Sample Vol. 10μL Sample Vol. 10μL Samples 1. Gallic acid 40μg/mL Samples 1. Gallic acid 40μg/mL 2. Protocatechuic acid 20μg/mL 20μg/mL 2. Protocatechuic acid 20μg/mL 20μg/mL 3. Phthalic acid 100μg/mL 3. Gentisic acid 100μg/mL 4. Gentisic acid 100μg/mL 4. Phthalic acid 100μg/mL 5. Salicylic acid 200μg/mL 5. Salicylic acid 200μg/mL

FOOD 37 F084 F085 Separation of Malachite green and Leucomalachite green Separation of Malachite green and Leucomalachite green by LC/MS

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18MS 150-2.0mmØ 5μm A: 0.01mol/L Ammonium formate Eluent A: 0.01mol/L Ammonium formate Eluent B: Acetonitrile B: Acetonitrile

Flow Rate 2.0mL/min. Flow Rate 0.2mL/min. Detection DAD: 580nm (0-13min.), 265nm (13-45min.) Detection MS ESI+ Temperature 40°C Temperature 40°C Sample Vol. 10μL Sample Vol. 2μL Samples 1. Malachite green 0.05mg/mL Samples 1. Malachite green 0.05mg/mL 2. Leucomalachite green 0.05mg/mL 2. Leucomalachite green 0.05mg/mL

F086 Separation of Sudan

Column Mightysil RP-18GP 250-4.6mmØ 5μm Mightysil RP-18GP 150-4.6mmØ 5μm Eluent A : Acetonitrile/ Methanol=85/15 B : Acetonitrile/ 0.1(v/v%)Formic acid=90/10 Flow Rate 1.0mL/min. Detection UV500nm Temperature 40°C Sample Vol. 10μL Samples 1. Para red 2. Sudan Ⅰ 3. Sudan Ⅱ 4. Sudan Ⅲ 5. Sudan Ⅳ Each 5μg/mL

38 FOOD F087 Separation of Sudan by LC/MS

Column Mightysil RP-18MS 150-2.0mmØ 5μm Eluent Acetonitrile/ 0.1%(v/v)Formic acid = Flow Rate 0.2mL / min. Detection MS ESI+ Temperature 40°C Sample Vol. 10μL Samples 1. Para red 2. Sudan Ⅰ 3. Sudan Ⅱ 4. Sudan Ⅲ 5. Sudan Ⅳ Each 5μg/mL

F052 Separation of Aflatoxin G1, G2, B1, B2

Column Mightysil RP-18 250-4.6mmØ 5μm Eluent Methanol/ Water = 1/1 Flow Rate 0.75mL/min. Detection Fluorescence 450nm (Excitation 365nm) Temperature Room Temperature Sample Vol. 20μL Samples Mixture of samples were pre-treated with trifluoroacetic acid in order to convert G1 and B1 to their derivatives 1. Aflatoxin G1 derivative 20ng/mL 2. Aflatoxin B1 derivative 20ng/mL 3. Aflatoxin G2 20ng/mL 4. Aflatoxin B2 20ng/mL

FOOD 39 F081 Separation of Fumonisins

Column Mightysil RP-18 MS 150-2.0mmØ 5μm Eluent A:Acetonitrile B:0.1%Formic acid Linear gradient: 0min A25% → 5min A50% → 8min A50% → 10min A25% → 25min A25% Flow Rate 0.2mL/min. Detection MS ESI+ Temperature 40°C Sample Vol. 2μL Samples 1. Fumonisin B1 2. Fumonisin B3 3. Fumonisin B2 Each 50ppb in Acetonitrile/Water= 50/ 50

F088 F079 Separation of Melamine Separation of Rutrefactive amines

Column Mightysil RP-18GP 250-4.6mmØ 5μm Column Mightysil RP-8 GP 250-4.6mmØ 5μm Eluent Acetonitorile /Water = 60/40

Eluent Buffer(pH 3.0)*/Acetonitrile=85/15 Flow Rate 1.0mL/min. [*10 mM Citric acid + 10 mM Sodium octane sulfonate, pH adjust : Detection Fluorescence 525nm (Excitation 325nm) NaOH] Temperature 40°C Flow Rate 1.0mL/min. Sample Vol. 5μL Detection UV240nm Samples 1. Putrescine 10μg/mL Temperature 40°C 2. Cadaverine 10μg/mL Sample Vol. 10μL 3. Histamine 250μg/mL Samples 1. Ammelide 4. Tyramine 50μg/mL 2. Ammeline 5. Spermidine 10μg/mL 3. Melamine Each 0.1mg/mL I.S : 1,6-Hexanediamine

40 FOOD ENVIRONMENT 41 42 ENVIRONMENT E001 Separation of N-Methylcarbamate derivatives-1

Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent A: Water, B: Acetonitrile Flow Rate 1.0mL/min. Detection Fluorescence 445nm (Excitation 339nm) Condition of post labeling Temperature 40°C Reaction Coil 5m-0.5mmØ Sample Vol. 10μL Teflon Samples 1. Aldicarb sulfoxide Hydrolyzed by 0.05M NaOH 2. Oxamyl solution 3. Methomyl Reagents Mixture of 10mg/L 4. Ethiofencarb sulfoxide o-phthalaldehyde in 50mM of Na B O 5. Ethiofencarb sulfone 2 4 7 solution 6. Carbofuran-3-keto and 50μL/L of 7. Carbofuran Mercaptoethanol 8. Ethiofencarb Flow Rate 0.5mL/min. 9. Isoprocarb Reaction 80°C 10. Methiocarb Temperature

E002 Separation of N-Methylcarbamate derivatives-2

Column Mightysil RP-18 250-4.6mmØ 5μm Eluent A: Water B: Tetrahydrofuran C: Acetonitrile Flow Rate 2.0mL/min. Condition of post labeling Detection Fluorescence 445nm Reaction Coil 5m-0.5mmØ (Excitation 339nm) Teflon Hydrolyzed by 0.05M NaOH Temperature 40°C solution Sample Vol. 10μL Reagents Mixture of 10mg/L Samples 1. Aldicarb sulfone o-phthalaldehyde 2. Ethiofencarb sulfone in 50mM of Na2B4O7 3. Aldicarb solution 4. Propoxur and 50μL/L of Mercaptoethanol 5. Carbofuran 6. Bendiocarb Flow Rate 0.3mL/min. 7. Carbaryl Reaction 80°C Temperature

ENVIRONMENT 43 E003 E004 Separation of Oxine-Copper and Asulam Separation of Oxine-Copper and Thiram

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ 20mM Ammonium acetate buffer (pH3.80*) Eluent Acetonitrile/ 20mM Phoshoric acid = 5/95 [*pH adjust : conc.Acetic acid] = 25/75 Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV250nm 0.005AUFS Detection UV250nm 0.02AUFS Temperature Room Temperature Temperature 40°C Sample Vol. 10μL Sample Vol. 10μL Samples 1. Oxine-Copper 0.08μg/mL Samples 1. Oxine-Copper 3μg/mL 2. Asulam 0.40μg/mL 2. Thiram 7μg/mL

E005 E006 Separation of Simazine, Thiram and Thiobencarb Separation of Thiram, Iprodione and Bensulide

Column Mightysil RP-18 150-4.6mmØ 5μm Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ 20mM Phosphate buffer(pH3.0)= 55/45 Eluent Acetonitrile/ Water = 60/40 Flow Rate 1.0mL/min. Flow Rate 0.8mL/min. Detection UV230nm 0.16AUFS Detection UV230nm 0.04AUFS Temperature 30°C Temperature Room Temperature Sample Vol. 20μL Sample Vol. 10μL Samples 1. Simazine 1μg/mL Samples 1. Thiram 4μg/mL 2. Thiram 1μg/mL 2. Iprodione 10μg/mL 3. Thiobencarb 1μg/mL 3. Bensulide 10μg/mL

44 ENVIRONMENT E007 E008 Separation of Thiram Separation of Thiophanate-methyl and its metabolite

Column Mightysil RP-18 150-4.6mmØ 5μm Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ Water = 50/50 Eluent Methanol/ 10mM Potassium dihydrogenphosphate = 1/1 Flow Rate 1.0mL/min. Flow Rate 0.8mL/min. Detection UV280nm 0.005AUFS Detection UV278nm Temperature 40°C Temperature 40°C Sample Vol. 10μL Samples 1. Methyl-2-benzimidazole carbamate Sample 1. Thiram 0.4μg/mL 2. Thiophanate-methyl

E009 E011 Separation of Fenitrothion and its metabolite Separation of Agricultural Chemicals (Germicides)

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ 10mM Potassium dihydrogenphosphate (pH3.5) = 55/45 Flow Rate 1.0mL/min. Column Mightysil RP-18 150-4.6mmØ 5μm Detection UV254nm 0.16AUFS Eluent Methanol/ Water = 60/40 Temperature Room Temperature Flow Rate 1.0mL/min. Sample Vol. 10μL Detection UV254nm Samples 1. Thiophanate-methyl 25μg/mL Temperature 40°C 2. Thiram 100μg/mL Sample Vol. 50μL 3. Diclomezine 50μg/mL Samples 1. Nitrocresol 500μg/mL 4. Triflumizole 25μg/mL 2. Fenitrothion 500μg/mL 5. Pencycuron 100μg/mL

ENVIRONMENT 45 E012 E013 Separation of Agricultural Chemicals (Pesticides) Separation of Agricultural Chemicals (Herbicides)-1

Column Mightysil RP-18 150-4.6mmØ 5μm Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile/ 10mM Potassium dihydrogenphosphate (pH3.5) = 65/35 Eluent Acetonitrile/ Water = 75/25 Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV225nm 0.16AUFS Detection UV254nm 0.16AUFS Temperature Room Temperature Temperature Room Temperature Sample Vol. 12μL Sample Vol. 10μL Samples 1. Dicamba 20μg/mL Samples 1. Imidacloprid 25μg/mL 2. Bensulfuron-methyl 30μg/mL 2. Fenobucarb 100μg/mL 3. Mefenacet 10μg/mL 3. Hexythiazox 300μg/mL 4. Bromobutide 150μg/mL 4. Fluvalinate 60μg/mL 5. Pyributicarb 50μg/mL

E014 E015 Separation of Agricultural Chemicals (Herbicides)-2 Separation of 5 Agricultural Chemicals -1

Column Mightysil RP-18 150-4.6mmØ 5μm

Eluent A: Acetonitrile/10mM Potassium dihydrogenphosphate (pH2.2) = 5/95 Column Mightysil RP-18 150-4.6mmØ 5μm B: Acetonitrile/10mM Potassium dihydrogenphosphate (pH2.2) = 80/20 Eluent Acetonitrile/ Water = 55/45 Linear gradient from 100% of A to 100% of B for 30 min. Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV254nm 0.16AUFS Detection UV240nm 0.04AUFS Temperature Room Temperature Temperature Room Temperature Sample Vol. 7.5μL Sample Vol. 3μL Samples 1. Diuron 25μg/mL Samples 1. Oxine-Copper 40μg/mL 2. Propanil 25μg/mL 2. Asulam 100μg/mL 3. Dymron (Daimuron) 75μg/mL 3. Thiram 15μg/mL 4. Chlorpropham 250μg/mL 4. Iprodione 100μg/mL 5. Pyrazolate 125μg/mL 5. Bensulide 200μg/mL

46 ENVIRONMENT E016 E028 Separation of 5 Agricultural Chemicals -2 Separation of 5 Agricultural Chemicals - 3

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18 (L) GP 150-4.6mmØ 5μm

Eluent Acetonitrile/ 10mM Ammonium acetate buffer(pH3.3*)= 7/ 13 [*pH Acetonitrile/ 10mM Pottasium dihydrogen phosphate Buffer adjust : conc.Acetic acid] Eluent (pH3.80*) = 1/3

Flow Rate 1.0mL/min. [*pH adjust : H3PO4] Detection UV270nm(0-3min.), UV240nm(3-5.5min.), UV230nm(5.5-14min.) Flow rate 1.0mL/min. Temperature 40°C Temperature 40°C Sample Vol. 5μL Sample Vol. 20μL Samples 1. Asulam 10μg/mL Samples 1. Asulam 270nm, 1μg/mL 2. Oxine-Copper 10μg/mL Detection 2. Oxine-Copper 240nm, 1μg/mL 3. Triclopyr 10μg/mL 3. Mecoprop 230nm, 1μg/mL 4. Thiram 10μg/mL 4. Triclopyr 230nm, 1μg/mL 5. Mecoprop 10μg/mL 5. Thiram 270nm, 1μg/mL

E029 E034 Separation of 5 Agricultural Chemicals – 4 Separation of 5 Agricultural Chemicals – 5

Column Mightysil RP-18 (L) GP 150-4.6mmØ 5μm

Eluent Acetonitrile / Potassium Phosphate Buffer* = 50/50 [*1.02g of Potassium dihydrogenphosphate was dissolved in 300mL Column Mightysil RP-18GP 150-4.6mmØ 5μm

water, then adjusted pH to 3.5 with H3PO4] Eluent A; Water, B; Acetonitrile C; Methanol Flow rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV230nm Detection UV270nm Temperature 40°C Temperature 40°C Sample Vol. 10μL Sample Vol. 40μL Samples 1. Oxine-Copper 5μg/mL Samples 1. Imidacloprid 0.1μg/mL Detection 2. Mecoprop 5μg/mL 2. Benomyl 0.1μg/mL 3. Benomyl 1μg/mL 3. Thiophanate-methyl 0.1μg/mL 4. Iprodione 5μg/mL 4. Carbaryl 0.1μg/mL 5. Pencycuron 5μg/mL 5. Linuron 0.1μg/mL

ENVIRONMENT 47 E017 E018 Separation of 6 Agricultural Chemicals Separation of 9 Agricultural Chemicals

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Acetonitrile Methanol/ 50mM Potassium dihydrogenphosphate Column Mightysil RP-18 150-4.6mmØ 5μm (pH3.2) = 43/ 9/ 48

Eluent A: Acetonitrile/ 10mM Potassium dihydrogenphosphate (pH2.2) = 5/95 Flow Rate 1.0mL/min. B: Acetonitrile/ 10mM Potassium dihydrogenphosphate (pH2.2) = 80/20 Detection 270nm(0-2.2min.), 240nm(2.2-10min.), 230nm(10-22min.), Linear gradient from 100% of A to 100% of B for 30 min. 220nm(22-27min.), 240nm(27min.-) Flow Rate 1.0mL/min. Temperature 40°C Detection UV240nm 0.16AUFS Sample Vol. 5μL Temperature Room Temperature Samples 1. Asulam 10μg/mL 6. Chlorothalonil 10μg/mL Sample Vol. 10μL 2. Oxine-Copper 10μg/mL 7. Iprodione 10μg/mL Samples 1. Oxine-Copper 20μg/mL 4. Thiram 20μg/mL 3. Triclopyr 10μg/mL 8. Bensulide 10μg/mL 2. Asulam 120μg/mL 5. Iprodione 120μg/mL 4. Thiram 10μg/mL 9. Pencycuron 10μg/mL 3. Simazine 20μg/mL 6. Bensulide 240μg/mL 5. Mecoprop 10μg/mL

E030 Separation of 12 Agricultural Chemicals

Column Mightysil RP-18 (L) GP 150-4.6mmØ 5μm

Eluent Acetonitrile/ 10mM Pottasium dihydrogen phosphate Buffer (pH3.80*) = 1/3

[*pH adjust : H3PO4]

Flow rate 1.0mL/min. Detection 0~6min.: 270nm, 6~10.9min.: 240nm, 10.9~24min.: 230nm, 24min.~: 270nm Temperature 35°C Sample Vol. 10μL Samples 1. Asulam 2. Oxine-Copper Detection 3. Simazine 4. Mecoprop 5. Thiram 6. Siduron 7. Propyzamide 8. Iprodione 9. Chlorothalonil 10. Pencycuron 11. Bensulide 12. Butamifos Each 10μg/mL

48 ENVIRONMENT E035 Separation of 9 Agricultural Chemicals – 2

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent A; Water, B; Acetonitrile Eluent A; Water, B; Acetonitrile Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV230nm Detection UV230nm Temperature 40°C Temperature 40°C Sample Vol. 10μL Sample Vol. 10μL Samples 1. hiabendazole Samples 1. hiabendazole 2. Triadimenol 2. Triadimenol 3. Diflubenzuron 4. Bitertanol 3. Diflubenzuron 5. Pyrazoxyfen 6. Triflumizole metabolite 4. Bitertanol 7. Pyrazolate 8. Teflubenzuron 5. Pyrazoxyfen 9. Chlorfluazuron Each 25μg/mL 6. Triflumizole metabolite

E036 Separation of 3 Agricultural Chemicals

Column Mightysil RP-18GP 150-4.6mmØ 5μm

Eluent A; 10mM Potassium dihydrogenphosphate(pH3.50*) [pH adjust :H3PO4] B; Acetonitrile

Flow Rate 1.0mL/min. Detection UV254nm 0.02AUFS Temperature 40°C Sample Vol. 10μL Sample 1. Destosylpyrazolate 2.5μg/mL 2. Dithianon 2.5μg/mL 3. Triflumizole 2.5μg/mL

ENVIRONMENT 49 E045 Separation of Pesticides standard solution 24 [A golf course pesticide]

Column Mightysil RP-18PA 150-4.6mmØ 5μm Eluent Acetonitrile/25mM Phosphate Buffer(pH3.3)=7/13 Flow Rate 1.0mL/min. Detection UV270nm (0~2.5min), UV240nm (2.5~5.0min) UV230nm (5.0~13.0min), UV240nm (13.0~16.0min) UV255nm (16.0~19.5min), UV245nm (19.5~25.0min) UV235nm (25.0~35.0min) Temperature 40°C Sample Vol. 5μL Samples 34023-96 Pesticides standard solution 24 1. Asulam 2. Oxine-Copper 3. Triclopyr 4. Thiram 5. Mecoprop 6. Flazasulfuron 7. Siduron 8. Halosulfuron-methyl 9. Azoxystrobin Each 10ppm

E019 F027 Separation of Intermediates of Agricultural Chemicals Separation of Carbofuran (halogenized Benzoic acids)

Column Mightysil RP-18 250-4.6mmØ 5μm Eluent Acetonitrile/ 0.1% Trifluoroacetic acid = 40/60 Flow Rate 1.0mL/min. Detection UV254nm 0.256AUFS Temperature Room Temperature Sample Vol. 10μL Samples Column Mightysil RP-18GP 150-4.6mmØ 5μm 1mg/mL each Eluent Acetonitrile/ Water = 30/70 Flow rate 1.0mL/min. Detection Fluorescence 307nm (Excitation 279nm) Temperature 40°C Sample Vol. 50μL Sample 1. Carbofuran 0.1μg/mL

50 ENVIRONMENT E032 E033 Separation of Acephate Separation of Imazalil in Concentrated Lemonade Juice

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18GP 150-4.6mmØ 5μm Eluent Acetonitrile/ Water = 5/95 Eluent Methanol/ 20mM Disodium hydrogen phosphate dihydrate = 3/1 Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV214nm Detection UV230nm Temperature 40°C Temperature 40°C Sample Vol. 20μL Sample Vol. 20μL Sample 1. Acephate 1mg/mL Sample 1. Imazalil 5mg/mL inLemonade Juice

E040 E041 Separation of Pesticides standard solution 28 Separation of 3 Agricultural Chemicals

Column Mightysil RP-18PA 150-4.6mmØ 5μm Eluent 2-Propanol/ Water = 30/70 Flow Rate 1.0mL/min. Detection FL Ex : 339nm Column Mightysil RP-18PA 250-4.6mmØ 5μm Em : 455nm Eluent Acetonitrile/ 50mM Potassium dihydrogenphosphate(pH3.3*) = 55/ Temperature 40°C

45[*pH adjust : H3PO4] Post-column reaction sysytem Flow Rate 1.0mL/min. Reagent1: 50mM NaOH soln. 0.7mL/min Detection UV270nm Asulam Reaction coil 0.5mmID×5m, 80°C UV230nm thiophanate-methyl, siduron, iprodion Reagent2: 6mM o-Phtalaldehyde Temperature 40°C 30mM 2-Mercaptoethanol Sample Vol. 20μL 50mM Sodium Tetraborate Samples 34027-96 Pesticides standard solution 28 in Methanol/ Water= 1/ 100 0.7mL/min 1. Asulam 50ppm Sample Vol. 1μL 2. Thiophanate-methyl 100ppm Samples 1. Methomyl 3. Siduron 100ppm 2. Carbofuran 4. Iprodione 50ppm 3. Carbaryl Each 10ppm Cf. Complementary items (for water quality management) ; attached method 9 Cf. Complementary items(for water quality management) ; attached method 13

ENVIRONMENT 51 E042 E043 Separation of Carbaryl Separation of Diquat

Column Mightysil RP-18PA 150-4.6mmØ 5μm

Eluent 13.5mL/L Phosphoric acid Column Mightysil RP-18PA 150-4.6mmØ 5μm + 3.0g/L Sodium 1-pentanesulfonate Eluent Acetonitrile/ Water = 30/70 + 10mL/L Diethylamine in water Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection Fluorescence 307nm (Excitation 279nm) Detection UV313nm Temperature 40°C Temperature 40°C Sample Vol. 20μL Sample Vol. 20μL Samples 1. Carbaryl 1.0ppm Samples 1. Diquat 1.0ppm Cf. Complementary items(for water quality management) ; attached method 10 Cf. Complementary items(for water quality management) ; attached method 11

E044 E039 Separation of Iminoctadine triacetate Separation of LAS

Column Mightysil RP-18PA 150-4.6mmØ 5μm Eluent Acetonitrile/ 100mM Sodium perchlorate Column Mightysil RP-18GP 250-4.6mmØ 5μm +0.05% Phosphoric acid in Water =5/17 Eluent 0.1M Sodium perchlorate/ Acetonitrile= 65/3 Flow Rate 0.7mL/min. Flow Rate 1.0mL/min. Detection FL Ex 395nm, Em 500nm Detection UV225nm Temperature 40°C Temperature 40°C Post-column reaction sysytem Sample Vol. 10μL Reagent1: 500mM NaOH soln. 0.2mL/min Samples 24289-96 LAS standard stock solution 1000ppm (C10-14 total) Reaction coil 0.5mmID×5m, 80°C n-Decylbenzenesulfonic acid sodium salt (C10) Reagent2: 3g/L Ninhydrin soln. 0.2mL/min n-Undecylbenzenesulfonic acid sodium salt (C11) Sample Vol. 20μL n-Dodecylbenzenesulfonic acid sodium salt (C12) Samples 1. Iminoctadine triacetate 1.0ppm n-Tridecylbenzenesulfonic acid sodium salt (C13) Cf. Complementary items(for water quality management) ; attached method 15 n-Tetradecylbenzenesulfonic acid sodium salt (C14)

52 ENVIRONMENT E010 Separation of 15 Synthetic Antibiotics

Column Mightysil RP-18GP 250-4.6mmØ 5μm Eluent A: Methanol/ 25mM Potassium dihydrogenphosphate = 15/85 B: Methanol/ 25mM Potassium dihydrogenphosphate = 70/30 Flow Rate 0.5mL/min. Detection Maximum wavelength detected by photodiode-array Temperature 40°C Sample Vol. 20μL Samples QCA: 2-Quinoxaline CDX: Carbadox PYR: Pyrimethamine carboxylic acid SMR: Sulfamerazine MRT: Morantel citrate DFZ: Difurazone FZD: Furazolidone SDM: Sulfadimethoxine NAA: Nalidixic acid SDD: Sulfadimidine SQX: Sulfaquinoxaline PMA: Piromidic acid SMM: Sulfamonomethoxine OXA: Oxolinic acid NCZ: Nicarbazin

E020 E023 Separation of Benzo[a]pyrene Separation of Microsystins

Column Mightysil RP-18 150-4.6mmØ 5μm Eluent Methanol/ 50mM Phosphate Buffer(pH3.0) = 58/42 Column Mightysil RP-18 150-4.6mmØ 5μm Flow Rate 1.0mL/min. Eluent Acetonitrile/ Water = 75/25 Detection UV240nm Flow Rate 1.0mL/min. Temperature Room Temperture Detection UV254nm Sample Vol. 20μL Temperature 40°C Samples 1. Microcystin RR 3.33μg/mL Sample Vol. 3μL 2. Microcystin YR 3.33μg/mL Sample 1. Benzo[a]pyrene 2mg/mL 3. Microcystin LR 3.33μg/mL

ENVIRONMENT 53 E046 E024 Separation of Patulin Separation of Bisphenol A -1

Column Mightysil RP-18GP 250-4.6mmØ 5μm

Eluent A: Acetonitrile/ Water = 7/3 Column Mightysil RP-18PA 250-4.6mmØ 5μm B: Acetonitrile Eluent Acetonitrile/0.082% Sodium perchlorate =3/97 Linear gradient from 100% of A to 100% of B for 33min. Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV276nm Detection UV217nm Temperature 40°C Temperature 40°C Sample Vol. 50μL Sample Vol. 20μL Samples 1. Patulin 0.05ppm Sample 1. Bisphenol A 0.625ppm (Assayed value)

E025 E026 Separation of Bisphenol A –2 Separation of Trace Level Bisphenol A

Column Mightysil RP-18GP 150-4.6mmØ 5μm Column Mightysil RP-18GP 250-4.6mmØ 5μm Eluent Acetonitrile/ Water = 35/65 Eluent Acetonitrile/ Water = 45/55 Flow Rate 1.0mL/min. Flow rate 0.9mL/min Detection UV254nm 0.01AUFS Detection UV225nm Temperature 40°C Temperature 45°C Sample Vol. 100μL Sample Vol. 100μL Samples 1. Phenol 2.5ppm Samples 1. Phenol 0.01μg/mL 2. Bisphenol A 2.5ppm 2. Bisphenol A 0.01μg/mL 3. 4-tert-Butylphenol 2.5ppm 3. 4-tert-Butylphenol 0.01μg/mL

54 ENVIRONMENT E031 E021 Separation of 4-Vinylguaiacol Separation of DNPH-Aldehydes-1

Column Mightysil RP-18GP 250-4.6mmØ 5μm

Eluent Phosphate Buffer*/ Methanol = 50/50 Column Mightysil RP-18 250-4.6mmØ 5μm [*4.90g/L phosphoric acid/ 6.80g/L Pottasium dihydrogen Eluent Acetonitrile/ Water = 60/40 phosphate = 1/2] Flow Rate 1.0mL/min. Flow Rate 1.0mL/min. Detection UV360nm Detection UV260nm Temperature 40°C Temperature 40°C Sample Vol. 20μL Sample Vol. 5μL Samples 1. Formaldehyde-2,4-DNPH 1μg/mL (as HCHO)

Sample 1. 4-Vinylguaiacol 19μg/mL 2. Acetoaldehyde-2,4-DNPH 1μg/mL (as CH3CHO)

E022 Separation of DNPH-Aldehydes-2

Column Mightysil RP-18 250-4.6mmØ 5μm Eluent Acetonitrile/ Water = 75/25 Flow Rate 1.0mL/min. Detection UV385nm Temperature 40°C Sample Vol. 1μL Samples 1. Formaldehyde-2,4-DNPH 100μg/mL 2. Acetaldehyde-2,4-DNPH 100μg/mL 3. Acrolein-2,4-DNPH 100μg/mL 4. Methylethylketone-2,4-DNPH 200μg/mL 5. Methylisobutylketone-2,4-DNPH 200μg/mL 6. Isophorone-2,4-DNPH 200μg/mL

ENVIRONMENT 55 E037 Separation of DNPH-Aldehydes-3

Column Mightysil DNPH 250-4.6mmØ 5μm Eluent A: Water B: Methanol Flow Rate 1.0mL/min. Detection UV360nm Temperature 40°C Sample Vol. 10μL Samples 1. Formaldehyde-DNPH 6. Crotonaldehyde-DNPH 2. Acetaldehyde-DNPH 7. Butylaldehyde-DNPH 3. Acrolein-DNPH 8. Benzaldehyde-DNPH 4. Acetone-DNPH 9. iso-Valeraldehyde-DNPH 5. Propionaldehyde-DNPH 10. Valeraldehyde-DNPH Each 1ppm

E038 Separation of DNPH-Aldehydes-4

Column Mightysil DNPH 250-4.6mmØ 5μm Eluent A: Water B: Acetonitrile Flow Rate 1.0mL/min. Detection UV360nm Temperature 40°C Sample Vol. 10μL Samples 1. Formaldehyde-DNPH 6. Crotonaldehyde-DNPH Each 1ppm 2. Acetaldehyde-DNPH 7. Butylaldehyde-DNPH 3. Acrolein-DNPH 8. Benzaldehyde-DNPH 4. Acetone-DNPH 9. iso-Valeraldehyde-DNPH 5. Propionaldehyde-DNPH 10. Valeraldehyde-DNPH 01106-96 Aldehydes-2,4-DNPH standard stock solution Ⅱ

56 ENVIRONMENT INDEX

CONPOUND NAME SHEET NO. (PAGE) CONPOUND NAME SHEET NO. (PAGE) A Bisphenol A E024 (54), E025 (54), E026 (54) Acephate E032 (51) Bitertanol E035 (49) Acesulfame K F069 (25), F080 (25) Blue-1, Brilliant blue FCF F038 (22), F040 (23), F072 (24) Acetaldehyde-2,4-DNPH E021 (55), E022 (55), E037 (56), E038 (56) Blue-1 Aluminium Lake, Brilliant blue FCF Aluminium Lake F041 (23) Acetic acid F098 (36) Blue-2, Indigocarmine F038 (22), F040 (23), F072 (24) Acetone-2,4-DNPH E037 (56), E038 (56) Brilliant blue FCF, Blue-1 F038 (22), F040 (23), F072 (24) Acid red, Red-106 F039 (22), F040 (23), F072 (24) Brilliant blue FCF Aluminium Lake, Blue-1 Aluminium Lake F041 (23) Acrolein-2,4-DNPH E022 (55), E037 (56), E038 (56) Bromobutide E013 (46) Acrylic acid F036 (36), F094 (36), F098 (36) Butamifos E030 (48) Acylanthocyanin F060 (24) Butylaldehyde-2,4-DNPH E037 (56), E038 (56) Adipic acid F032 (35), F092 (35) 2-tert-Butyl hydroquinone, BHQ F042 (20), F073 (20) Aflatoxin B1 F052 (39) Butyl hydroquinone, 2-tert-Butyl hydroquinone F042 (20), F073 (20) Aflatoxin B2 F052 (39) tert-Butylhydroxyanisole, BHA F042 (20), F073 (20) Aflatoxin G1 F052 (39) Butyl hydroxyanisole, tert-Butylhydroxyanisole F042 (20), F073 (20) Aflatoxin G2 F052 (39) Butyl hydroxytoluene, BHT F042 (20), F073 (20) Albendazole F023 (17) n-Butyl paraben, n-Butyl p-hydroxybenzoate F043 (18), F074 (19) Aldicarb E002 (43) 2,6-di-tert-Butyl-p-cresol, Butyl hydroxytoluene F042 (20), F073 (20) Aldicarb sulfone E002 (43) 4-tert-Butylphenol E025 (54), E026 (54) Aldicarb sulfoxide E001 (43) n-Butyl p-hydroxybenzoate, n-Butyl paraben F043 (18), F074 (19), F077 (19) Allura Red AC, Red-40 F072 (24) Allyl isothiocyanate F063 (29) C Amaranth, Red-2 F039 (22), F040 (23), F072 (24) Cadaverine F079 (40)

Ammelide F088 (40) Caffeine F050 (24), F053 (27), F054 (27), F055 (28), F056 (28)

Ammeline F088 (40) Capsaicin F065 (29)

Amprolium F011 (11), F012 (11) Carbadox E010 (53), F013 (12), F026 (13)

Anthocyanin F060 (24) Carbaryl E002 (43), E034 (47), E041 (51), E042 (52)

Apigenin F099 (30) Carbofuran E001 (43), E002 (43), E027 (50), E041 (51)

Ascorbic acid F048 (21) Carbofuran-3-keto E001 (43)

L-Ascorbic acid, Vitamin C F058 (08), F070 (07) β-Carotene F004 (09), F059 (09), F071 (08)

Aspartame F050 (24), F080 (25) Catechin F053 (27), F054 (27), F055 (28), F056 (28)

Asulam E003 (44), E015 (46), E016 (47), E017 (48), E018 (48), Catechin gallate F054 (27), F055 (28), F056 (28)

E028 (47), E030 (48), E040 (51), E045 (50) Chlorfluazuron E035 (49)

Avoparcin mixture F031 (17) Chlorophyll a F056 (28)

Azoxystrobin E045 (50) Chlorothalonil E018 (48), E030 (48)

Chlorpropham E014 (46) B Chlortetracycline F006 (13) Bendiocarb E002 (43) Cholecalciferol, Vitamin D3 F003 (07), F005 (06)

Benofloxacin F007 (09) Chrysin F103 (31) Benomyl E029 (47), E034 (47) Citric acid F035 (35), F036 (36), F093 (35), F094 (36), F098 (36) Bensulfuron methyl E013 (46) Clopidol F013 (12) Bensulide E006 (44), E015 (46), E017 (48), E018 (48), E030 (48) Closantel F014 (14), F020 (16) Benzaldehyde-2,4-DNPH E037 (56), E038 (56) Coenzyme Q10, Ubiquinone 10 F089 (26) Benzo[a]pyrene E020 (53) Coenzyme Q9, Ubiquinone 9 F089 (26) 5,6-Benzoflavone F100 (30) Crotonaldehyde-2,4-DNPH E037 (56), E038 (56) 7,8-Benzoflavone F101 (31) β-Cryptoxanthin F071 (08) Benzoic acid F044 (18), F047(18), F050 (24), F061 (20), F075 (18) ,F077(19) Curcumin F082 (29), F083 (30) Benzoyl peroxide F091 (26) Cyanocobalamin, Vitamin B12 F097 (08) BHA, tert-Butylhydroxyanisole F042 (20), F073 (20) Cyclamic acid F076 (25) BHQ, 2-tert-Butyl hydroquinone F042 (20), F073 (20) BHT, Butyl hydroxytoluene F042 (20), F073 (20) D Biochanin A F102 (31) Daimuron, Dymron E014 (46)

Biphenyl F045 (21), F046 (21) Danofloxacin F007 (09)

INDEX 57 INDEX

CONPOUND NAME SHEET NO. (PAGE) CONPOUND NAME SHEET NO. (PAGE)

Danofloxacin mesylate F008 (10) Formaldehyde-2,4-DNPH E021 (55), E022 (55), E037 (56), E038 (56) n-Decylbenzenesulfonic acid sodium salt E039 (52) Formic acid F062 (36) Dehydroacetic acid F044 (18), F047 (18), F075 (18) Fumaric acid F036 (36), F094 (36), F098 (36) Destosylpyrazolate E036 (49) Fumonisin B1 F081 (40) DG, Dodecyl gallate F073 (20) Fumonisin B2 F081 (40) Dicamba E013 (46) Fumonisin B3 F081 (40) Diclomezine E011 (45) Furazolidone E010 (53), F010 (11), F013 (12) Diflubenzuron E035 (49) Difurazone E010 (53), F013 (12) G Dihydrocapsaicin F065 (29) Gallic acid F034 (37), F054 (27), F095 (37), F096 (37) 2,5-Dimethylpyrazine F051 (26) Gallocatechin gallate F054(27), F055 (28), F056 (28) Diosmin F104 (31) Gentisic acid F034 (37), F095 (37), F096 (37) Diquat E043 (52) Gleen-3, Fast green FCF F038 (22), F040 (23), F072 (24) Dithianon E036 (49) Glucose F066 (34), F068 (34) Diuron E014 (46) Glutaric acid F032 (35), F092 (35) n-Dodecylbenzenesulfonic acid sodium salt E039 (52) Glycolic acid F035 (35), F093 (35), F098 (36) Dodecyl gallate, DG F073 (20) Doxycycline F006 (13) H Dymron, Daimuron E014 (46) Halogenated benzoic acid E019 (50) Halosulfuron-methyl E045 (50) E Hesperetin F106 (32) Enrofloxacin F007 (09), F008 (10) Hesperidin F107 (32)

Eosin F072 (24) 1,6-Hexanediamine F079 (40)

Eosin Y F039 (22), F040 (23) Hexythiazox E012 (46)

Epicatechin F053 (27), F054 (27), F055 (28), F056 (28) Histamine F079 (40)

Epicatechin gallate F053 (27), F054 (27), F055 (28), F056 (28) HMBP, Hydroxymethylbutylphenol F073 (20)

Epigallocatechin F053 (27), F054 (27), F055 (28), F056 (28) Hydroxymethylbutylphenol, HMBP F073 (20)

Epigallocatechin gallate F053 (27), F054 (27), F055 (28), F056 (28) 5-Hydroxythiabendazole F015 (14), F030 (16)

Ergocalciferol, Vitamin D2 F003 (07), F005 (06) Erythorbic acid F048 (21) I Erythrosine, Red-3 F039 (22), F040 (23), F072 (24) Imazalil E033 (51), F045 (21)

Erythrosine Aluminium Lake, Red-3 Aluminium Lake F041 (23) Imidacloprid E012 (46), E034 (47)

Ethiofencarb E001 (43) Iminoctadine triacetate E044 (52)

Ethiofencarb sulfone E001 (43), E002 (43) Indigocarmine, Blue-2 F038 (22), F040 (23), F072 (24)

Ethiofencarb sulfoxide E001 (43) Iprodione E006 (44), E015 (46), E017 (48), E018 (48),

Ethopabate F011 (11) E029 (47), E030 (48), E040 (51)

Ethyl p-hydroxybenzoate, Ethyl paraben F043 (18), F069 (25), F074 (19), Isobutyl p-hydroxybenzoate, Isobutyl paraben F043 (18), F074 (19), F077 (19)

F077(19) Isobutyl paraben, Isobutyl p-hydroxybenzoate F043 (18), F074 (19), F077 (19) Ethyl paraben, Ethyl p-hydroxybenzoate F043 (18), F069 (25), F074 (19), Isometamidium F025 (17) F077 (19) Isophorone-2,4-DNPH E022 (55) Isoprocarb E001 (43) F Isopropyl p-hydroxybenzoate, Isopropyl paraben F043 (18), F074 (19), F077(19) Fast green FCF, Gleen-3 F038 (22), F040 (23), F072 (24) Isopropyl paraben, Isopropyl p-hydroxybenzoate F043 (18), F074 (19), F077 (19) Fenethylcyanate F063 (29) Isoquercitrin F108 (32) Fenitrothion E009 (45) iso-Valeraldehyde-2,4-DNPH E037 (56), E038 (56) Fenobucarb E012 (46) Ivermectin F014 (14), F018 (15) Flavonol F105 (32) Flazasulfuron E045 (50) K Flubendazole F014 (14), F022 (16) Kaempferol F109 (33) Flumequine F007 (09) Fluvalinate E012 (46) L Folic acid, Vitamin B9 F001 (05), F067 (06) Lactic acid F035 (35), F093 (35), F098 (36)

58 INDEX INDEX

CONPOUND NAME SHEET NO. (PAGE) CONPOUND NAME SHEET NO. (PAGE)

LAS E039 (52) Olaquindox F013 (12) Leucomalachite green F084 (38), F085 (38) Orbifloxacin F007 (09) Linuron E034 (47) Ormethoprim F013 (12) Lutein F071(08) Oxalic acid F033 (37), F035 (35), F036 (36), F093 (35), F094 (36) Luteolin F110 (33) Oxamyl E001 (43) Lycopene F071 (08) Oxine-copper E003 (44), E004 (44), E015 (46), E016 (47), E017 (48), E018 (48), E028 (47), E029 (47), E030 (48), E045 (50) M Oxolinic acid E010 (53), F007 (09), F012 (11), F013 (12) Malachite green F084 (38), F085 (38) Oxytetracycline F006 (13), F014 (14), F019 (14) Maleic acid F036 (36), F094 (36), F098 (36) Malic acid F035 (35), F036 (36), F093 (35), F094 (36), F098 (36) P Malonic acid F032 (35), F036 (36), F092 (35), F094 (36), F098 (36) Pantothenic acid calcium salt F002 (05), F067 (06) Maltoheptaose F066 (34) Para red F086 (38), F087 (39) Maltohexaose F066 (34) Patulin E046 (54) Maltopentaose F066 (34) Pencycuron E011 (45), E018 (48), E029 (47), E030 (48) Maltose F066 (34) 2,2,5,7,8-Pentamethylhydroxychroman F057 (07) Maltotetraose F066 (34) PG, Propyl gallate F073 (20) Maltotriose F066 (34) Phenol E025 (54), E026 (54) Mecoprop E016 (47), E018 (48), E028 (47), E029 (47), E030 (48), E045 (50) o-Phenylphenol F045 (21), F046 (21) Mefenacet E013 (46) Phloxine, Red-104 F039 (22), F040 (23), F072 (24)

Melamine F088 (40) Phthalic acid F034 (37), F095 (37), F096 (37)

Menadione, Vitamin K3 F005 (06) Phylloquinone, Vitamin K1 F005 (06)

Methiocarb E001 (43) Pimelic acid F032 (35), F092 (35)

Methomyl E001 (43), E041 (51) Piromidic acid E010 (53), F007 (09), F013 (12) p-Methylacetophenon F064 (29) Propanil E014 (46)

Methyl-2-benzimidazole carbamate E008 (45) Propionaldehyde-2,4-DNPH E037 (56), E038 (56)

Methylethylketone-2,4-DNPH E022 (55) Propoxur E002 (43)

Methyl p-hydroxybenzoate, Methyl paraben F043 (18), F074 (19) Propyl gallate, PG F073 (20)

Methylisobutylketone-2,4-DNPH E022 (55) n-Propyl p-hydroxybenzoate, n-Propyl paraben F043 (18), F074 (19), F077 (19)

Methyl paraben, Methyl p-hydroxybenzoate F043 (18), F074 (19) n-Propyl paraben, n-Propyl p-hydroxybenzoate F043 (18), F074 (19), F077 (19)

2-Methylpyrazine F051 (26) 5-Propylsulfonyl-1H-benzimidazole-2-amine F015 (14), F024 (17)

Microcystin E023 (53) Propyzamide E030 (48)

Morantel citrate E010 (53), F013 (12) Protocatechuic acid F034 (37), F095 (37), F096 (37)

Putrescine F079 (40) N Pyrazin F051 (26) Nalidixic acid E010 (53), F007 (09), F013 (12) Pyrazolate E014 (46), E035 (49)

Naringenin F111 (33) Pyrazoxyfen E035 (49)

Naringin F112 (33) Pyributicarb E013 (46) NDGA, Nordihydroguaiaretic acid F073 (20) Pyridoxine hydrochloride, Vitamin B6 F001 (05), F002 (05), F067 (06) New coccine, Red-102 F039 (22), F040 (23), F072 (24) Pyrimethamine E010 (53), F011 (11), F013 (12) Nicarbazin E010 (53), F011 (11), F013 (12) Nicotinamide F001 (05), F002 (05), F067 (06) Q Nicotinic acid F001 (05), F067 (06) Quassin F064 (29) Nitric acid F033 (37) Quercetin F078 (30) Nitrocresol E009 (45) 2-Quinoxalinecarboxylic acid E010 (53), F015 (14), F027 (13) Nitrofurazone F010 (11) Nordihydroguaiaretic acid, NDGA F073 (20) R Raffinose F068 (34) O Red-102, New coccine F039 (22), F040 (23), F072 (24) Octyl gallate, OG F073 (20) Red-104, Phloxine F039 (22), F040 (23), F072 (24) Ofloxacin F007 (09) Red-105, Rose bengal F039 (22), F040 (23), F072 (24) OG, Octyl gallate F073 (20) Red-106, Acid red F039 (22), F040 (23), F072 (24)

INDEX 59 INDEX

CONPOUND NAME SHEET NO. (PAGE) CONPOUND NAME SHEET NO. (PAGE)

Red-2, Amaranth F039 (22), F040 (23), F072 (24) Thiram E004 (44)E005 (44), E006 (44), E007 (45), E011 (45), Red-3, Erythrosine F039 (22), F040 (23), F072 (24) E015 (46), E016 (47), E017 (48), E 018 (48), E028 (47), E030 (48), E045 (50) Red-3 Aluminium Lake, Erythrosine Aluminium Lake F041 (23) Tocopherol, Vitamin E F005 (06), F058 (08) Red-40, Allura Red AC F072 (24) α-Tocopherol F057 (07) Retinol acetate, Vitamin A acetate F005 (06) β-Tocopherol F057 (07) Rhoifolin F113 (34) γ-Tocopherol F057 (07) Riboflavin, Vitamin B2 F001 (05), F002 (05), F067 (06) δ-Tocopherol F057 (07) Riboflavin phosphate sodium salt F001 (05), F067 (06) Tocopherol acetate, Vitamin E acetate F005 (06) Rose bengal, Red-105 F039 (22), F040 (23), F072 (24) α-Trenbolone F014 (14), F016 (15) Rutin F078 (30) β-Trenbolone F014 (14), F017 (15) Triadimenol E035 (49) S Triclopyr E016 (47), E018 (48), E028 (47), E045 (50) Saccharin F049 (25), F061 (20), F077 (19) n-Tridecylbenzenesulfonic acid sodium salt E039 (52) Salicylic acid F034 (37), F075 (18), F095 (37), F096 (37) Triflumizole E011 (45), E036 (49) Siduron E030 (48), E040 (51), E045 (50) Triflumizole metabolite E035 (49) Simazine E005 (44), E017 (48), E030 (48) Trihydroxybutyrophenone, THBP F073 (20) Sorbic acid F044 (18), F047 (18), F049 (25), F061 (20), 4,2',4'-Trihydroxychalcone F114 (34) F075 (18), F077 (19) Trimethoprim F013 (12) Spermidine 2,3,5-Trimethylpyrazine F051 (26) Succinic acid F032 (35), F092 (35), F098 (36) Tyramine F079 (40)

Sucrose F068 (34) Sudan Ⅰ F086(38), F087(39) U Sudan Ⅱ F086 (38), F087 (39) Ubiquinone 10, Coenzyme Q10 F089 (26)

Sudan Ⅲ F086(38), F087 (39) Ubiquinone 9, Coenzyme Q9 F089 (26)

Sudan Ⅳ F086 (38), F087 (39) n-Undecylbenzenesulfonic acid sodium salt E039 (52) Sulfachlorpyridazine F009(10) Sulfadimethoxine E010 (53), F009 (10), F 013 (12) V Sulfadimidine E010 (53), F013 (12), F015 (14), F028 (13) Valeraldehyde-2,4-DNPH E037 (56), E038 (56)

Sulfamerazine E010 (53), F009 (10), F013 (12) 4-Vinylguaiacol E031 (55)

Sulfamethoxazole F009 (10) Vitamin A acetate, Retinol acetate F005 (06)

Sulfamethoxypyridazine F009 (10) Vitamin B1, Thiamine hydrochloride F001 (05), F067 (06)

Sulfamonomethoxine E010 (53), F009 (10), F013 (12) Vitamin B12, Cyanocobalamin F097 (08)

Sulfaquinoxaline E010 (53), F013 (12) Vitamin B2, Riboflavin F001 (05), F002 (05), F067 (06)

Sulfathiazole F009 (10) Vitamin B6, Pyridoxine hydrochloride F001 (05), F002 (05), F067 (06)

Sulfisomidine F009 (10) Vitamin B9, Folic acid F001 (05), F067 (06)

Sunset yellow FCF, Yellow-5 F037 (21), F040 (23), F072 (24) Vitamin C, L-Ascorbic acid F058 (08), F070 (07)

Vitamin D2, Ergocalciferol F003 (07), F005 (06) T Vitamin D3, Cholecalciferol F003 (07), F005 (06) Tartaric acid F036 (36), F062 (36), F094 (36) Vitamin E, Tocopherol F005 (06), F058 (08) Tartrazine, Yellow-4 F037 (21), F040 (23), F072 (24) Vitamin E acetate, Tocopherol acetate F005 (06) Tartrazine Aluminium Lake, Yellow-4 Aluminium Lake F041 (23) Vitamin K1, Phylloquinone F005 (06) Teflubenzuron E035 (49) Vitamin K3, Menadione F005 (06) Tetracycline F006 (13) n-Tetradecylbenzenesulfonic acid sodium salt E039 (52) Y 2,3,5,6-Tetramethylpyrazine F051 (26) Yellow-4, Tartrazine F037 (21), F072 (24) THBP, Trihydroxybutyrophenone F073 (20) Yellow-5, Sunset yellow FCF F037 (21), F040 (23), F072 (24) Theogallin F054 (27) Thiabendazole E035 (49), F029 (16), F045 (21), F046 (21) Z Thiamine hydrochloride, Vitamin B1 F001 (05), F067 (06) Zeranol F014 (14), F021 (15) Thiobencarb E005 (44) DL-Thioctic acid F090 (26) Thiophanate-methyl E008 (45), E011 (45), E034 (47), E040 (51)

60 INDEX 02 CONTENTS