(Cyanobacterial Genera) 2014, Using a Polyphasic Approach
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Protocols for Monitoring Harmful Algal Blooms for Sustainable Aquaculture and Coastal Fisheries in Chile (Supplement Data)
Protocols for monitoring Harmful Algal Blooms for sustainable aquaculture and coastal fisheries in Chile (Supplement data) Provided by Kyoko Yarimizu, et al. Table S1. Phytoplankton Naming Dictionary: This dictionary was constructed from the species observed in Chilean coast water in the past combined with the IOC list. Each name was verified with the list provided by IFOP and online dictionaries, AlgaeBase (https://www.algaebase.org/) and WoRMS (http://www.marinespecies.org/). The list is subjected to be updated. Phylum Class Order Family Genus Species Ochrophyta Bacillariophyceae Achnanthales Achnanthaceae Achnanthes Achnanthes longipes Bacillariophyta Coscinodiscophyceae Coscinodiscales Heliopeltaceae Actinoptychus Actinoptychus spp. Dinoflagellata Dinophyceae Gymnodiniales Gymnodiniaceae Akashiwo Akashiwo sanguinea Dinoflagellata Dinophyceae Gymnodiniales Gymnodiniaceae Amphidinium Amphidinium spp. Ochrophyta Bacillariophyceae Naviculales Amphipleuraceae Amphiprora Amphiprora spp. Bacillariophyta Bacillariophyceae Thalassiophysales Catenulaceae Amphora Amphora spp. Cyanobacteria Cyanophyceae Nostocales Aphanizomenonaceae Anabaenopsis Anabaenopsis milleri Cyanobacteria Cyanophyceae Oscillatoriales Coleofasciculaceae Anagnostidinema Anagnostidinema amphibium Anagnostidinema Cyanobacteria Cyanophyceae Oscillatoriales Coleofasciculaceae Anagnostidinema lemmermannii Cyanobacteria Cyanophyceae Oscillatoriales Microcoleaceae Annamia Annamia toxica Cyanobacteria Cyanophyceae Nostocales Aphanizomenonaceae Aphanizomenon Aphanizomenon flos-aquae -
Biological Nitrogen Fixation Dr. Anuj Rani, Department of Botany, T.N.B
E- learning: B.Sc. Part-II, Botany Hons and Part-I Sub.] Biological Nitrogen Fixation Dr. Anuj Rani, Department of Botany, T.N.B. College, Bhagalpur Email: [email protected] Conversion of molecular nitrogen (N2) of the atmosphere into inorganic nitrogenous compounds such as nitrates or ammonia is called as nitrogen fixation. When this nitrogen fixation occurs through the agency of some living organisms, the process is called as biological nitrogen fixation in which atmospheric nitrogen is converted into ammonia. Not all the organisms have capacity to fix molecular nitrogen (N2) of the atmosphere. Only certain prokaryotic micro-organisms such as some free living bacteria, cyanobacteria (blue- green algae) and some of the prokaryotic micro- organisms in symbiotic association with other plants (mostly legumes) can fix atmospheric nitrogen. They can be grouped as follows: A. Free Living: 1. Autotrophic: (a) Aerobic e.g., some cyanobacteria (blue-green algae). All those blue-green algae which can fix atmospheric nitrogen usually contain heterocyst’s such as Nostoc, Anabaena, Tolypothrix, Aulosira, Calothrix etc. But all the heterocyst’s bearing blue-green algae may not be atm. nitrogen fixers. A few non- heterocystous blue-green algae such as Gloeotheca are also known to fix atm. N2. (b) Anaerobic e.g., certain bacteria such as Chromatium and Rhodospirillum. 2. Heterotrophic: (a) Aerobic e.g., certain bacteria such as Azotobacter, Azospirillum, Derxia and Beijerinckia. (b) Facultative e.g., certain bacteria such as Bacillus and Klebsiella. (c) Anaerobic e.g., certain bacteria such as Clostridium and Methanococcus. 1 B. Symbiotic: (a) Root Nodules of Leguminous Plants: Various types of bacteria called rhizobia associated with root nodules of legumes can fix atm. -
Additional Analysis of Cyanobacterial Polyamines Distributions Of
Microb. Resour. Syst. Dec.32(2 ):1792016 ─ 186, 2016 Vol. 32, No. 2 Additional analysis of cyanobacterial polyamines ─ Distributions of spermidine, homospermidine, spermine, and thermospermine within the phylum Cyanobacteria ─ Koei Hamana1)*, Takemitsu Furuchi2), Hidenori Hayashi1) and Masaru Niitsu2) 1)Faculty of Engineering, Maebashi Institute of Technology 460-1 Kamisadori-machi, Maebashi, Gunma 371-0816, Japan 2)Faculty of Pharmaceutical Sciences, Josai University, 1-1, Keyakidai, Sakado, Saitama 350-0295, Japan To further catalogue the distribution of cyanobacterial cellular polyamines, we used HPLC and HPGC to newly analyze the acid-extracted polyamines from 14 cyanobacteria. The colony-forming Nostoc verrucosum (“Ashitsuki”) and Nostoc commune (“Ishikurage”), as well as Anabaena species (Nostocales), contained homospermidine. The thermo-halotolerant Spirulina subsalsa var. salina (Spirulinales), as well as freshwater Spirulina strains, contained spermidine. Putrescine, spermidine, and homospermidine were found in freshwater colony-forming Aphanothece sacrum (“Suizenji-nori”), whereas the halotolerant Aphanothece halophytica and Microcystis species (Chroococcales) contained spermidine alone. In addition to putrescine, spermidine, homospermidine and agmatine, thermospermine was found as a major polyamine in haloalkaliphilic Arthrospira platensis (“Spirulina”) (Oscillatoriales). In the Synechococcales, chlorophyll b-containing Prochlorococcus marina contained spermidine, and chlorophyll d-containing Acaryochloris marina contained -
Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris Marina
crossmark Downloaded from Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina Guangyu E. Chen,a Andrew Hitchcock,a Philip J. Jackson,a,b Roy R. Chaudhuri,a Mark J. Dickman,b C. Neil Hunter,a Daniel P. Canniffea* a Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom ; ChELSI Institute, Department of Chemical and Biological http://jb.asm.org/ Engineering, University of Sheffield, Sheffield, United Kingdomb ABSTRACT The major photopigment of the cyanobacterium Acaryochloris marina is chlorophyll d, while its direct biosynthetic precursor, chlorophyll a, is also present in the cell. These pigments, along with the majority of chlorophylls utilized by oxygenic pho- totrophs, carry an ethyl group at the C-8 position of the molecule, having undergone reduction of a vinyl group during biosyn- thesis. Two unrelated classes of 8-vinyl reductase involved in the biosynthesis of chlorophylls are known to exist, BciA and BciB. The genome of Acaryochloris marina contains open reading frames (ORFs) encoding proteins displaying high sequence similar- on April 20, 2016 by PERIODICALS OFFICE, MAIN LIBRARY, UNIVERSITY OF SHEFFIELD ity to BciA or BciB, although they are annotated as genes involved in transcriptional control (nmrA) and methanogenesis (frhB), respectively. These genes were introduced into an 8-vinyl chlorophyll a-producing ⌬bciB strain of Synechocystis sp. strain PCC 6803, and both were shown to restore synthesis of the pigment with an ethyl group at C-8, demonstrating their activities as 8-vi- nyl reductases. We propose that nmrA and frhB be reassigned as bciA and bciB, respectively; transcript and proteomic analysis of Acaryochloris marina reveal that both bciA and bciB are expressed and their encoded proteins are present in the cell, possibly in order to ensure that all synthesized chlorophyll pigment carries an ethyl group at C-8. -
Functional Basis of Microorganism Classification
RESEARCH ARTICLE Functional Basis of Microorganism Classification Chengsheng Zhu1*, Tom O. Delmont2, Timothy M. Vogel2, Yana Bromberg1,3* 1 Department of Biochemistry and Microbiology, Rutgers University, New Brunswick, New Jersey, United States of America, 2 Environmental Microbial Genomics, Laboratoire Ampere, École Centrale de Lyon, Université de Lyon, Ecully, France, 3 Institute for Advanced Study, Technische Universität München, Garching, Germany * [email protected] (CZ); [email protected] (YB) Abstract Correctly identifying nearest “neighbors” of a given microorganism is important in industrial and clinical applications where close relationships imply similar treatment. Microbial classifi- cation based on similarity of physiological and genetic organism traits (polyphasic similarity) OPEN ACCESS is experimentally difficult and, arguably, subjective. Evolutionary relatedness, inferred from Citation: Zhu C, Delmont TO, Vogel TM, Bromberg Y phylogenetic markers, facilitates classification but does not guarantee functional identity (2015) Functional Basis of Microorganism Classification. PLoS Comput Biol 11(8): e1004472. between members of the same taxon or lack of similarity between different taxa. Using over doi:10.1371/journal.pcbi.1004472 thirteen hundred sequenced bacterial genomes, we built a novel function-based microor- Editor: Christine A. Orengo, University College ganism classification scheme, functional-repertoire similarity-based organism network London, UNITED KINGDOM (FuSiON; flattened to fusion). Our scheme is phenetic, based on a network of quantitatively Received: March 27, 2015 defined organism relationships across the known prokaryotic space. It correlates signifi- cantly with the current taxonomy, but the observed discrepancies reveal both (1) the incon- Accepted: July 21, 2015 sistency of functional diversity levels among different taxa and (2) an (unsurprising) bias Published: August 28, 2015 towards prioritizing, for classification purposes, relatively minor traits of particular interest to Copyright: © 2015 Zhu et al. -
Phycogeography of Freshwater Phytoplankton: Traditional Knowledge and New Molecular Tools
Hydrobiologia (2016) 764:3–27 DOI 10.1007/s10750-015-2259-4 PHYTOPLANKTON & SPATIAL GRADIENTS Review Paper Phycogeography of freshwater phytoplankton: traditional knowledge and new molecular tools Judit Padisa´k • Ga´bor Vasas • Ga´bor Borics Received: 29 November 2014 / Revised: 6 March 2015 / Accepted: 14 March 2015 / Published online: 31 March 2015 Ó Springer International Publishing Switzerland 2015 Abstract ‘‘Everything is everywhere, but environ- relevant for biogeography of freshwater phytoplank- ments selects.’’ Is this true? The cosmopolitan nature ton. The following topics are considered: dispersal of algae, including phytoplankton, has been highlight- agents and distances; survival strategies of species; ed in many textbooks and burnt into the minds of geographic distribution of different types; patterns of biologists during their studies. However, the accumu- invasions; tools of molecular genetics; and metabo- lating knowledge on the occurrence of individual lomics to explore dispersal patterns, island biogeog- phytoplankton species in habitats where they have not raphy, and associated species–area relationships for been seen before, reports on invasive phytoplankton algae. species, and the increasing number of papers with phylogenetic trees and tracing secondary metabolites, Keywords Distribution Á Dispersal Á Invasion Á especially cyanotoxins, contradict. Phytoplankton Island biogeography Á Genomics Á Bloom-forming species, with rare exceptions, are neither cosmopoli- cyanobacteria tan, nor ubiquists. In this review paper, -
DOMAIN Bacteria PHYLUM Cyanobacteria
DOMAIN Bacteria PHYLUM Cyanobacteria D Bacteria Cyanobacteria P C Chroobacteria Hormogoneae Cyanobacteria O Chroococcales Oscillatoriales Nostocales Stigonematales Sub I Sub III Sub IV F Homoeotrichaceae Chamaesiphonaceae Ammatoideaceae Microchaetaceae Borzinemataceae Family I Family I Family I Chroococcaceae Borziaceae Nostocaceae Capsosiraceae Dermocarpellaceae Gomontiellaceae Rivulariaceae Chlorogloeopsaceae Entophysalidaceae Oscillatoriaceae Scytonemataceae Fischerellaceae Gloeobacteraceae Phormidiaceae Loriellaceae Hydrococcaceae Pseudanabaenaceae Mastigocladaceae Hyellaceae Schizotrichaceae Nostochopsaceae Merismopediaceae Stigonemataceae Microsystaceae Synechococcaceae Xenococcaceae S-F Homoeotrichoideae Note: Families shown in green color above have breakout charts G Cyanocomperia Dactylococcopsis Prochlorothrix Cyanospira Prochlorococcus Prochloron S Amphithrix Cyanocomperia africana Desmonema Ercegovicia Halomicronema Halospirulina Leptobasis Lichen Palaeopleurocapsa Phormidiochaete Physactis Key to Vertical Axis Planktotricoides D=Domain; P=Phylum; C=Class; O=Order; F=Family Polychlamydum S-F=Sub-Family; G=Genus; S=Species; S-S=Sub-Species Pulvinaria Schmidlea Sphaerocavum Taxa are from the Taxonomicon, using Systema Natura 2000 . Triochocoleus http://www.taxonomy.nl/Taxonomicon/TaxonTree.aspx?id=71022 S-S Desmonema wrangelii Palaeopleurocapsa wopfnerii Pulvinaria suecica Key Genera D Bacteria Cyanobacteria P C Chroobacteria Hormogoneae Cyanobacteria O Chroococcales Oscillatoriales Nostocales Stigonematales Sub I Sub III Sub -
Research Journal of Pharmaceutical, Biological and Chemical Sciences
ISSN: 0975-8585 Research Journal of Pharmaceutical, Biological and Chemical Sciences A Novel Approach to Enhancement of Poly-Β-Hydroxybutyrate Accumulation Aulosira Fertilissima by Mixotrophy And Chemohetertrophy. 1S Sirohi*, 2N Mallick, 3SPS Sirohi , 1PK Tyagi, and 1GD Tripathi. 1Department of Biotechnology, MIET Meerut-250005, India. 2Department of Agricultural and Food Engineering, IIT Kharagpur-721302, India. 3Kisan PG Collage Simbhaoli, Gaziabad, India. ABSTRACT Aulisira fertilissima, a unicellular cyanobacterium, produced poly-β-hydroxybutyrate (PHB) up to 5.4% (w/w) dry cells when grown photoautotrophically but 8.9% when grown mixotrophically with 0.2% (w/v) glucose and acetate after 24 days. Gas-exchange limitations under mixotrophy and chemoheterotrophy with 0.2% (w/v) acetate and glucose enhanced the accumulation up to 17–19% (w/w) dry cells, the value almost 4- fold higher with respect to photoautotrophic condition. These results revealed high potential of Aulisira fertilissima in accumulating PHB, an appropriate raw material for biodegradable and biocompatible plastic. PHB could be an important material for plastic and pharmaceutical industries. Keywords: chemoheterotrophy, mixotrophy, Aulosira fertilissima, poly-β-hydroxybutyrate *Corresponding author March – April 2015 RJPBCS 6(2) Page No. 1266 ISSN: 0975-8585 INTRODUCTION Infact, Polyhydroxyalkanoates (PHAs) are the polymers of hydroxyalkanoates, which has gained tremendous impetus in the recent years because of its biodegradable and biocompatible nature and can be produced from renewable sources. PHAs are accumulated as a carbon and energy storage material in various microorganisms usually under the condition of limiting nutritional elements such as N, P, S, O, or Mg [1] in the presence of excess carbon [2]. Many of these bacterial species produce the polymer up to 20% of the dry cell weight (dcw) and a few, such as, Ralstonia eutropha, now called as Wautersia eutropha, is capable of accumulating poly-β-hydroxybutyrate (PHB) up to almost 80% of the dcw [3]. -
First Insights Into the Impacts of Benthic Cyanobacterial Mats on Fish
www.nature.com/scientificreports OPEN First insights into the impacts of benthic cyanobacterial mats on fsh herbivory functions on a nearshore coral reef Amanda K. Ford 1,2*, Petra M. Visser 3, Maria J. van Herk3, Evelien Jongepier 4 & Victor Bonito5 Benthic cyanobacterial mats (BCMs) are becoming increasingly common on coral reefs. In Fiji, blooms generally occur in nearshore areas during warm months but some are starting to prevail through cold months. Many fundamental knowledge gaps about BCM proliferation remain, including their composition and how they infuence reef processes. This study examined a seasonal BCM bloom occurring in a 17-year-old no-take inshore reef area in Fiji. Surveys quantifed the coverage of various BCM-types and estimated the biomass of key herbivorous fsh functional groups. Using remote video observations, we compared fsh herbivory (bite rates) on substrate covered primarily by BCMs (> 50%) to substrate lacking BCMs (< 10%) and looked for indications of fsh (opportunistically) consuming BCMs. Samples of diferent BCM-types were analysed by microscopy and next-generation amplicon sequencing (16S rRNA). In total, BCMs covered 51 ± 4% (mean ± s.e.m) of the benthos. Herbivorous fsh biomass was relatively high (212 ± 36 kg/ha) with good representation across functional groups. Bite rates were signifcantly reduced on BCM-dominated substratum, and no fsh were unambiguously observed consuming BCMs. Seven diferent BCM-types were identifed, with most containing a complex consortium of cyanobacteria. These results provide insight into BCM composition and impacts on inshore Pacifc reefs. Tough scarcely mentioned in the literature a decade ago, benthic cyanobacterial mats (BCMs) are receiving increasing attention from researchers and managers as being a nuisance on tropical coral reefs worldwide1–4. -
Cyanobacterial Nitrogen Fixation in the Baltic Sea with Focus on Aphanizomenon Sp
Cyanobacterial Nitrogen Fixation in the Baltic Sea With focus on Aphanizomenon sp. Jennie Barthel Svedén ©Jennie Barthel Svedén, Stockholm University 2016 Cover: Baltic Sea Aphanizomenon sp. colonies, modified from original photo by Helena Höglander ISBN 978-91-7649-481-3 Printed in Sweden by Holmbergs, Malmö 2016 Distributor: Department of Ecology, Environment and Plant Sciences Till mamma och pappa ABSTRACT Cyanobacteria are widely distributed in marine, freshwater and terrestrial habitats. Some cyanobacterial genera can convert di-nitrogen gas (N2) to bioavailable ammo- nium, i.e. perform nitrogen (N) fixation, and are therefore of profound significance for N cycling. N fixation by summer blooms of cyanobacteria is one of the largest sources of new N for the Baltic Sea. This thesis investigated N fixation by cyanobac- teria in the Baltic Sea and explored the fate of fixed N at different spatial and temporal scales. In Paper I, we measured cell-specific N fixation by Aphanizomenon sp. at 10 ºC, early in the season. Fixation rates were high and comparable to those in late sum- mer, indicating that Aphanizomenon sp. is an important contributor to N fixation al- ready in its early growth season. In Paper II, we studied fixation and release of N by Aphanizomenon sp. and found that about half of the fixed N was rapidly released and transferred to other species, including autotrophic and heterotrophic bacteria, diatoms and copepods. In Paper III, we followed the development of a cyanobacterial bloom and related changes in dissolved and particulate N pools in the upper mixed surface layer. The bloom-associated total N (TN) increase was mainly due to higher particu- late organic N (PON) concentrations, but also to increases in dissolved organic nitro- gen (DON). -
Spirulina Platensis an “Ultimate Food”: a Review
© 2019 IJRAR March 2019, Volume 6, Issue 1 www.ijrar.org (E-ISSN 2348-1269, P- ISSN 2349-5138) Spirulina Platensis an “Ultimate Food”: A Review 1Akshita Sharma, 2 Kamalpreet Kaur, 3 Manjri, 4 Deepti Marwaha 1,4Assistant Professor, 2,3Research Scholar 1Department of Biotechnology, 1Guru Nanak Girls College, Model town, Ludhiana, India. Introduction: Spirulina platensis is non toxic blue green algae which is filamentous cyanobacteria taken by the human as dietary supplement and use it as a food. Spirulina platensis is a biomass which is dried form of Arthrospira platensis .This blue green algae is the primary diet in humans, animals and aquatic life. Spirulina platensis is easily digested due to absence of cell wall .This blue green algae is an important diet in humans and in animals. In humans it is used as a source of protein and vitamin without causes any harmful effect. It is rich in phenolic acids, tocopherol and y-linolenic acids [1]. Spirulina platensis is most commonly available and widely used genus which has been extensively studied in different fields specially food and medicine [2]. Spirulina platensis was originally discovered in ponds and lakes. Spirulina platensis has been proven to boost the immune system, bolster the energy and reduce the risk of many cancers and infection. The nutritional quantity of the Blue-green algae Spirulina platensis has been evaluated on the basis of its chemical and amino acid composition and feeding trails with rats [3]. Due to high content of highly valuable proteins, indispensable amino acids, vitamins, beta carotene and other pigments, mineral substances, indispensable fatty acids and polysaccharides, Spirulina platensis has been found suitable for use as bioactive additives [4]. -
Production, Purification, and Study of the Amino Acid Composition of Microalgae Proteins
molecules Article Production, Purification, and Study of the Amino Acid Composition of Microalgae Proteins Anna Andreeva 1, Ekaterina Budenkova 1, Olga Babich 1 , Stanislav Sukhikh 1,2, Elena Ulrikh 3, Svetlana Ivanova 4,5,* , Alexander Prosekov 6 and Vyacheslav Dolganyuk 1,2 1 Institute of Living Systems, Immanuel Kant Baltic Federal University, 236016 Kaliningrad, Russia; [email protected] (A.A.); [email protected] (E.B.); [email protected] (O.B.); [email protected] (S.S.); [email protected] (V.D.) 2 Department of Bionanotechnology, Kemerovo State University, 650043 Kemerovo, Russia 3 Kuzbass State Agricultural Academy, 650056 Kemerovo, Russia; [email protected] 4 Natural Nutraceutical Biotesting Laboratory, Kemerovo State University, 650043 Kemerovo, Russia 5 Department of General Mathematics and Informatics, Kemerovo State University, 650043 Kemerovo, Russia 6 Laboratory of Biocatalysis, Kemerovo State University, 650043 Kemerovo, Russia; [email protected] * Correspondence: [email protected]; Tel.: +7-384-239-6832 Abstract: Microalgae are known to be rich in protein. In this study, we aim to investigate methods of producing and purifying proteins of 98 microalgae including Chlorella vulgaris, Arthrospira platensis, Nostoc sp., Dunaliella salina, and Pleurochrysis carterae (Baltic Sea). Therefore, we studied their amino acid composition and developed a two-stage protein concentrate purification method from the microalgae biomass. After an additional stage of purification, the mass fraction of protein substances with a molecular weight greater than 50 kDa in the protein concentrate isolated from the biomass of the microalga Dunaliella salina increased by 2.58 times as compared with the mass fraction before Citation: Andreeva, A.; Budenkova, filtration.