EIF3H Antibody

Total Page：16

File Type：pdf, Size：1020Kb

Product Datasheet EIF3H Antibody Catalog No: #36437 Orders: [email protected] Description Support: [email protected] Product Name EIF3H Antibody Host Species Rabbit Clonality Polyclonal Purification Antigen affinity purification. Applications IHC Species Reactivity Hu Specificity The antibody detects endogenous levels of total EIF3H protein. Immunogen Type Recombinant Protein Immunogen Description Fusion protein corresponding to residues near the C terminal of human eukaryotic translation initiation factor 3, subunit H Target Name EIF3H Other Names EIF3S3; eIF3-p40; eIF3-gamma Accession No. Swiss-Prot#: O15372NCBI Gene ID: 8667Gene Accssion: BC000386 Concentration 1.7mg/ml Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol. Storage Store at -20°C Application Details Immunohistochemistry: 1:50-1:200 Images Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using #36437 at dilution 1/40. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Immunohistochemical analysis of paraffin-embedded Human brain tissue using #36437 at dilution 1/40. Background Eukaryotic translation initiation factor 3 subunit H?is a?protein?that in humans is encoded by the?EIF3H?gene. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP: methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Note: This product is for in vitro research use only and is not intended for use in humans or animals. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2.

Copy Link

Recommended publications

Multiplexed Single-Cell Transcriptional Response Profiling to Define Cancer
ARTICLE https://doi.org/10.1038/s41467-020-17440-w OPEN Multiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action James M. McFarland 1,11, Brenton R. Paolella 1,11, Allison Warren1, Kathryn Geiger-Schuller 1,2, Tsukasa Shibue1, Michael Rothberg1, Olena Kuksenko1,2, William N. Colgan 1, Andrew Jones1, Emily Chambers1, Danielle Dionne1,2, Samantha Bender1, Brian M. Wolpin3,4,5, Mahmoud Ghandi 1, Itay Tirosh2,6, Orit Rozenblatt-Rosen1,2, Jennifer A. Roth1, Todd R. Golub 1,3,7,8, Aviv Regev 1,2,8,9,10, ✉ ✉ ✉ Andrew J. Aguirre 1,3,4,5,12 , Francisca Vazquez 1,12 & Aviad Tsherniak 1,12 1234567890():,; Assays to study cancer cell responses to pharmacologic or genetic perturbations are typically restricted to using simple phenotypic readouts such as proliferation rate. Information-rich assays, such as gene-expression profiling, have generally not permitted efficient profiling of a given perturbation across multiple cellular contexts. Here, we develop MIX-Seq, a method for multiplexed transcriptional profiling of post-perturbation responses across a mixture of samples with single-cell resolution, using SNP-based computational demultiplexing of single- cell RNA-sequencing data. We show that MIX-Seq can be used to profile responses to chemical or genetic perturbations across pools of 100 or more cancer cell lines. We combine it with Cell Hashing to further multiplex additional experimental conditions, such as post- treatment time points or drug doses. Analyzing the high-content readout of scRNA-seq reveals both shared and context-specific transcriptional response components that can identify drug mechanism of action and enable prediction of long-term cell viability from short- term transcriptional responses to treatment.
[Show full text]
Genes with 5' Terminal Oligopyrimidine Tracts Preferentially Escape Global Suppression of Translation by the SARS-Cov-2 NSP1 Protein
Downloaded from rnajournal.cshlp.org on September 28, 2021 - Published by Cold Spring Harbor Laboratory Press Genes with 5′ terminal oligopyrimidine tracts preferentially escape global suppression of translation by the SARS-CoV-2 Nsp1 protein Shilpa Raoa, Ian Hoskinsa, Tori Tonna, P. Daniela Garciaa, Hakan Ozadama, Elif Sarinay Cenika, Can Cenika,1 a Department of Molecular Biosciences, University of Texas at Austin, Austin, TX 78712, USA 1Corresponding author: [email protected] Key words: SARS-CoV-2, Nsp1, MeTAFlow, translation, ribosome profiling, RNA-Seq, 5′ TOP, Ribo-Seq, gene expression 1 Downloaded from rnajournal.cshlp.org on September 28, 2021 - Published by Cold Spring Harbor Laboratory Press Abstract Viruses rely on the host translation machinery to synthesize their own proteins. Consequently, they have evolved varied mechanisms to co-opt host translation for their survival. SARS-CoV-2 relies on a non-structural protein, Nsp1, for shutting down host translation. However, it is currently unknown how viral proteins and host factors critical for viral replication can escape a global shutdown of host translation. Here, using a novel FACS-based assay called MeTAFlow, we report a dose-dependent reduction in both nascent protein synthesis and mRNA abundance in cells expressing Nsp1. We perform RNA-Seq and matched ribosome profiling experiments to identify gene-specific changes both at the mRNA expression and translation level. We discover that a functionally-coherent subset of human genes are preferentially translated in the context of Nsp1 expression. These genes include the translation machinery components, RNA binding proteins, and others important for viral pathogenicity. Importantly, we uncovered a remarkable enrichment of 5′ terminal oligo-pyrimidine (TOP) tracts among preferentially translated genes.
[Show full text]
Systematically Profiling the Expression of Eif3 Subunits in Glioma Reveals
Chai et al. Cancer Cell Int (2019) 19:155 https://doi.org/10.1186/s12935-019-0867-1 Cancer Cell International PRIMARY RESEARCH Open Access Systematically profling the expression of eIF3 subunits in glioma reveals the expression of eIF3i has prognostic value in IDH-mutant lower grade glioma Rui‑Chao Chai1,4,6†, Ning Wang2†, Yu‑Zhou Chang3, Ke‑Nan Zhang1,6, Jing‑Jun Li1,6, Jun‑Jie Niu5, Fan Wu1,6*, Yu‑Qing Liu1,6* and Yong‑Zhi Wang1,3,4,6* Abstract Background: Abnormal expression of the eukaryotic initiation factor 3 (eIF3) subunits plays critical roles in tumo‑ rigenesis and progression, and also has potential prognostic value in cancers. However, the expression and clinical implications of eIF3 subunits in glioma remain unknown. Methods: Expression data of eIF3 for patients with gliomas were obtained from the Chinese Glioma Genome Atlas (CGGA) (n 272) and The Cancer Genome Atlas (TCGA) (n 595). Cox regression, the receiver operating characteristic (ROC) curves= and Kaplan–Meier analysis were used to study= the prognostic value. Gene oncology (GO) and gene set enrichment analysis (GSEA) were utilized for functional prediction. Results: In both the CGGA and TCGA datasets, the expression levels of eIF3d, eIF3e, eIF3f, eIF3h and eIF3l highly were associated with the IDH mutant status of gliomas. The expression of eIF3b, eIF3i, eIF3k and eIF3m was increased with the tumor grade, and was associated with poorer overall survival [All Hazard ratio (HR) > 1 and P < 0.05]. By contrast, the expression of eIF3a and eIF3l was decreased in higher grade gliomas and was associated with better overall sur‑ vival (Both HR < 1 and P < 0.05).
[Show full text]
Translation Initiation Factor Eif3h Targets Specific Transcripts To
Translation initiation factor eIF3h targets specific transcripts to polysomes during embryogenesis Avik Choudhuria,b, Umadas Maitraa,1, and Todd Evansb,1 aDepartment of Developmental and Molecular Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, NY 10461; and bDepartment of Surgery, Weill Cornell Medical College, New York, NY 10065 Edited by Igor B. Dawid, The Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, and approved April 30, 2013 (received for review February 14, 2013) Eukaryotic translation initiation factor 3 (eIF3) plays a central role eukaryotes. These—eIF3d, eIF3e, eIF3f, eIF3h, eIF3j, eIF3k, in translation initiation and consists of five core (conserved) sub- eIF3l, and eIF3m—were designated “non-core” subunits (4). units present in both budding yeast and higher eukaryotes. Higher In contrast to the budding yeast, the genome of the fission eukaryotic eIF3 contains additional (noncore or nonconserved) yeast Schizosaccharomyces pombe contains structural homologs subunits of poorly defined function, including sub-unit h (eIF3h), of at least five noncore (nonconserved) eIF3 subunits—eIF3d, which in zebrafish is encoded by two distinct genes (eif3ha and eIF3e, eIF3f, eIF3h, and eIF3m. The gene encoding eIF3f is eif3hb). Previously we showed that eif3ha encodes the predominant essential for growth, whereas eIF3d, eIF3e, and eIF3h are dis- isoform during zebrafish embryogenesis and that depletion of this pensable for growth and viability (5–11). However, deleted factor causes defects in the development of the brain and eyes. To strains show specific phenotypes including defects in meiosis/ investigate the molecular mechanism governing this regulation, we sporulation (6, 9, 11).
[Show full text]
Relevance of Translation Initiation in Diffuse Glioma Biology and Its
cells Review Relevance of Translation Initiation in Diﬀuse Glioma Biology and its Therapeutic Potential Digregorio Marina 1, Lombard Arnaud 1,2, Lumapat Paul Noel 1, Scholtes Felix 1,2, Rogister Bernard 1,3 and Coppieters Natacha 1,* 1 Laboratory of Nervous System Disorders and Therapy, GIGA-Neurosciences Research Centre, University of Liège, 4000 Liège, Belgium; [email protected] (D.M.); [email protected] (L.A.); [email protected] (L.P.N.); [email protected] (S.F.); [email protected] (R.B.) 2 Department of Neurosurgery, CHU of Liège, 4000 Liège, Belgium 3 Department of Neurology, CHU of Liège, 4000 Liège, Belgium * Correspondence: [email protected] Received: 18 October 2019; Accepted: 26 November 2019; Published: 29 November 2019 Abstract: Cancer cells are continually exposed to environmental stressors forcing them to adapt their protein production to survive. The translational machinery can be recruited by malignant cells to synthesize proteins required to promote their survival, even in times of high physiological and pathological stress. This phenomenon has been described in several cancers including in gliomas. Abnormal regulation of translation has encouraged the development of new therapeutics targeting the protein synthesis pathway. This approach could be meaningful for glioma given the fact that the median survival following diagnosis of the highest grade of glioma remains short despite current therapy. The identiﬁcation of new targets for the development of novel therapeutics is therefore needed in order to improve this devastating overall survival rate. This review discusses current literature on translation in gliomas with a focus on the initiation step covering both the cap-dependent and cap-independent modes of initiation.
[Show full text]
New Partners Identified by Mass Spectrometry Assay Reveal Functions of NCAM2 in Neural Cytoskeleton Organization
International Journal of Molecular Sciences Article New Partners Identiﬁed by Mass Spectrometry Assay Reveal Functions of NCAM2 in Neural Cytoskeleton Organization Antoni Parcerisas 1,2,3,*,† , Alba Ortega-Gascó 1,2,† , Marc Hernaiz-Llorens 1,2 , Maria Antonia Odena 4, Fausto Ulloa 1,2, Eliandre de Oliveira 4, Miquel Bosch 3 , Lluís Pujadas 1,2 and Eduardo Soriano 1,2,* 1 Department of Cell Biology, Physiology and Immunology, University of Barcelona and Institute of Neurosciences, 08028 Barcelona, Spain; [email protected] (A.O.-G.); [email protected] (M.H.-L.); [email protected] (F.U.); [email protected] (L.P.) 2 Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), 28031 Madrid, Spain 3 Department of Basic Sciences, Universitat Internacional de Catalunya, 08195 Sant Cugat del Vallès, Spain; [email protected] 4 Plataforma de Proteòmica, Parc Cientíﬁc de Barcelona (PCB), 08028 Barcelona, Spain; [email protected] (M.A.O.); [email protected] (E.d.O.) * Correspondence: [email protected] (A.P.); [email protected] (E.S.) † A.P. and A.O.-G. contributed equally. Abstract: Neuronal cell adhesion molecule 2 (NCAM2) is a membrane protein with an important role in the morphological development of neurons. In the cortex and the hippocampus, NCAM2 is essential for proper neuronal differentiation, dendritic and axonal outgrowth and synapse forma- tion. However, little is known about NCAM2 functional mechanisms and its interactive partners Citation: Parcerisas, A.; during brain development. Here we used mass spectrometry to study the molecular interactome Ortega-Gascó, A.; Hernaiz-Llorens, of NCAM2 in the second postnatal week of the mouse cerebral cortex.
[Show full text]
This Thesis Has Been Submitted in Fulfilment of the Requirements for a Postgraduate Degree (E.G
This thesis has been submitted in fulfilment of the requirements for a postgraduate degree (e.g. PhD, MPhil, DClinPsychol) at the University of Edinburgh. Please note the following terms and conditions of use: • This work is protected by copyright and other intellectual property rights, which are retained by the thesis author, unless otherwise stated. • A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. • This thesis cannot be reproduced or quoted extensively from without first obtaining permission in writing from the author. • The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the author. • When referring to this work, full bibliographic details including the author, title, awarding institution and date of the thesis must be given. Investigating the roles of Translation Elongation Factor 1B in mammalian cells Yuan Cao Thesis submitted for the degree of Doctor of Philosophy The University of Edinburgh 2012 Declaration I here declare that this thesis has been composed by me and that all the work presented within is my own, unless clearly indicated otherwise. This work has not been submitted for any other degree or professional qualification. Yuan Cao i Acknowledgements First of all I wish to express my deepest gratitude to my supervisor Professor Cathy Abbott who has been so patient and understanding in the last four years. I appreciate her scientific guidance throughout my research, her encouragement when I was being negative, as well as her support and help in both my work and life.
[Show full text]
(Eifs) in Endometrial Cancer
International Journal of Molecular Sciences Article The Prognostic Significance of Eukaryotic Translation Initiation Factors (eIFs) in Endometrial Cancer Maria Anna Smolle 1,2 , Piotr Czapiewski 3,4, Sylwia Lapi ńska-Szumczyk 5, Hanna Majewska 3, Anna Supernat 6, Anna Zaczek 6 , Wojciech Biernat 3, Nicole Golob-Schwarzl 2,7 and Johannes Haybaeck 2,4,7,8,* 1 Department of Orthopaedics and Trauma, Medical University of Graz, Auenbruggerplatz 5, 8036 Graz, Austria; [email protected] 2 Area 2 Cancer, Center for Biomarker Research in Medicine, Stiftingtalstraße 5, 8010 Graz, Austria; [email protected] 3 Department of Pathomorphology, Medical University of Gdansk, Mariana Smoluchowskiego 17, 80-214 Gdańsk,Poland; [email protected] (P.C.); [email protected] (H.M.); [email protected] (W.B.) 4 Department of Pathology, Medical Faculty, Otto-von-Guericke University Magdeburg, Leipziger Straße 44, 39120 Magdeburg, Germany 5 Department of Gynaecology, Gynaecological Oncology and Gynaecological Endocrinology, Medical University of Gdańsk,M. Skłodowskiej-Curie 3a Street, 80-210 Gdańsk,Poland; [email protected] 6 Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańnskand Medical University of Gdańsk,Ba˙zyńskiego1a, 80-952 Gdańsk,Poland; [email protected] (A.S.); [email protected] (A.Z.) 7 Institute of Pathology, Medical University of Graz, Auenbruggerplatz 25, 8036 Graz, Austria 8 Department of Pathology, Neuropathology and Molecular Pathology, Medical University of Innsbruck, Müllerstraße 44, 6020 Innsbruck, Austria * Correspondence: [email protected]; Tel.: +49-391-67-15817 Received: 3 November 2019; Accepted: 5 December 2019; Published: 6 December 2019 Abstract: Whilst the role of eukaryotic translation initiation factors (eIFs) has already been investigated in several human cancers, their role in endometrial cancer (EC) is relatively unknown.
[Show full text]
Global Analysis of Trna and Translation Factor Expression Reveals a Dynamic Landscape of Translational Regulation in Human Cancers
ARTICLE https://doi.org/10.1038/s42003-018-0239-8 OPEN Global analysis of tRNA and translation factor expression reveals a dynamic landscape of translational regulation in human cancers Zhao Zhang1, Youqiong Ye1, Jing Gong1, Hang Ruan1, Chun-Jie Liu2, Yu Xiang1, Chunyan Cai3, An-Yuan Guo2, 1234567890():,; Jiqiang Ling4, Lixia Diao5, John N. Weinstein5 & Leng Han 1,6 The protein translational system, including transfer RNAs (tRNAs) and several categories of enzymes, plays a key role in regulating cell proliferation. Translation dysregulation also contributes to cancer development, though relatively little is known about the changes that occur to the translational system in cancer. Here, we present global analyses of tRNAs and three categories of enzymes involved in translational regulation in ~10,000 cancer patients across 31 cancer types from The Cancer Genome Atlas. By analyzing the expression levels of tRNAs at the gene, codon, and amino acid levels, we identified unequal alterations in tRNA expression, likely due to the uneven distribution of tRNAs decoding different codons. We find that overexpression of tRNAs recognizing codons with a low observed-over-expected ratio may overcome the translational bottleneck in tumorigenesis. We further observed overall overexpression and amplification of tRNA modification enzymes, aminoacyl-tRNA synthe- tases, and translation factors, which may play synergistic roles with overexpression of tRNAs to activate the translational systems across multiple cancer types. 1 Department of Biochemistry and Molecular Biology, McGovern Medical School at The University of Texas Health Science Center at Houston, Houston, TX 77030, USA. 2 Department of Bioinformatics and Systems Biology, Hubei Bioinformatics and Molecular Imaging Key Laboratory, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, 430074 Hubei, People’s Republic of China.
[Show full text]
The Global Translation Profile in a Ribosomal Protein Mutant Resembles That of an Eif3 Mutant
University of Massachusetts Medical School eScholarship@UMMS Open Access Articles Open Access Publications by UMMS Authors 2013-12-30 The global translation profile in a ribosomal protein mutant resembles that of an eIF3 mutant Bayu Sisay Tiruneh University of Tennessee - Knoxville Et al. Let us know how access to this document benefits ou.y Follow this and additional works at: https://escholarship.umassmed.edu/oapubs Part of the Genomics Commons, and the Microbiology Commons Repository Citation Tiruneh BS, Kim B, Gallie DR, Roy B, von Arnim AG. (2013). The global translation profile in a ribosomal protein mutant resembles that of an eIF3 mutant. Open Access Articles. https://doi.org/10.1186/ 1741-7007-11-123. Retrieved from https://escholarship.umassmed.edu/oapubs/2444 This material is brought to you by eScholarship@UMMS. It has been accepted for inclusion in Open Access Articles by an authorized administrator of eScholarship@UMMS. For more information, please contact [email protected]. Tiruneh et al. BMC Biology 2013, 11:123 http://www.biomedcentral.com/1741-7007/11/123 RESEARCH ARTICLE Open Access The global translation profile in a ribosomal protein mutant resembles that of an eIF3 mutant Bayu Sisay Tiruneh1, Byung-Hoon Kim1,2, Daniel R Gallie3, Bijoyita Roy1,4 and Albrecht G von Arnim1,5* Abstract Background: Genome-wide assays performed in Arabidopsis and other organisms have revealed that the translation status of mRNAs responds dramatically to different environmental stresses and genetic lesions in the translation apparatus. To identify additional features of the global landscape of translational control, we used microarray analysis of polysomal as well as non-polysomal mRNAs to examine the defects in translation in a poly(A) binding protein mutant, pab2 pab8, as well as in a mutant of a large ribosomal subunit protein, rpl24b/shortvalve1.
[Show full text]
Loss of Eif4e Phosphorylation Engenders Depression-Like Behaviors Via Selective Mrna Translation
2118 • The Journal of Neuroscience, February 21, 2018 • 38(8):2118–2133 Neurobiology of Disease Loss of eIF4E Phosphorylation Engenders Depression-like Behaviors via Selective mRNA Translation X Ineˆs S. Amorim,1,2* Sonal Kedia,1,2* XStella Kouloulia,1,2* XKonstanze Simbriger,1,2* XIlse Gantois,3 X Seyed Mehdi Jafarnejad,3 Yupeng Li,1,2 Agniete Kampaite,1,2 Tine Pooters,1 XNicola Romano`,1 and X Christos G. Gkogkas1,2,4 1Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh EH8 9XD, United Kingdom, 2Patrick Wild Centre, University of Edinburgh, Edinburgh EH8 9XD, United Kingdom, 3Goodman Cancer Research Centre and Biochemistry Department, McGill University, Montre´al, Quebec H3A 1A3, Canada, and 4Simons Initiative for the Developing Brain, University of Edinburgh, Edinburgh EH8 9XD, United Kingdom The MAPK/ERK (mitogen-activated protein kinases/extracellular signal-regulated kinase) pathway is a cardinal regulator of synaptic plasticity, learning, and memory in the hippocampus. One of major endpoints of this signaling cascade is the 5Ј mRNA cap binding protein eIF4E (eukaryotic Initiation Factor 4E), which is phosphorylated on Ser 209 by MNK (MAPK-interacting protein kinases) and controls mRNA translation. The precise role of phospho-eIF4E in the brain is yet to be determined. Herein, we demonstrate that ablation of eIF4E phosphorylation in male mice (4Eki mice) does not impair long-term spatial or contextual fear memory, or the late phase of LTP. Using unbiased translational profiling in mouse brain, we show that phospho-eIF4E differentially regulates the translation of a subset of mRNAs linked to inflammation, the extracellular matrix, pituitary hormones, and the serotonin pathway.
[Show full text]
Eukaryotic Translation Initiation Factors As Promising Targets in Cancer Therapy
Hao et al. Cell Communication and Signaling (2020) 18:175 https://doi.org/10.1186/s12964-020-00607-9 REVIEW Open Access Eukaryotic translation initiation factors as promising targets in cancer therapy Peiqi Hao1,2†, Jiaojiao Yu1†, Richard Ward3, Yin Liu2, Qiao Hao2,SuAn2* and Tianrui Xu2* Abstract The regulation of the translation of messenger RNA (mRNA) in eukaryotic cells is critical for gene expression, and occurs principally at the initiation phase which is mainly regulated by eukaryotic initiation factors (eIFs). eIFs are fundamental for the translation of mRNA and as such act as the primary targets of several signaling pathways to regulate gene expression. Mis-regulated mRNA expression is a common feature of tumorigenesis and the abnormal activity of eIF complexes triggered by upstream signaling pathways is detected in many tumors, leading to the selective translation of mRNA encoding proteins involved in tumorigenesis, metastasis, or resistance to anti-cancer drugs, and making eIFs a promising therapeutic target for various types of cancers. Here, we briefly outline our current understanding of the biology of eIFs, mainly focusing on the effects of several signaling pathways upon their functions and discuss their contributions to the initiation and progression of tumor growth. An overview of the progress in developing agents targeting the components of translation machinery for cancer treatment is also provided. Keywords: eIF, mRNA translation, Cancer, MAPK, PI3K/Akt, mTOR Background eukaryotes utilize many more initiation factors than do pro- The regulation of gene expression in eukaryotes can occur karyotes, reflecting the greater biological complexity of at different stages including gene transcription and mRNA eukaryotic translation.
[Show full text]