APPLICATION FORM Section 26 Determination
To obtain a determination of whether an organism is a new organism
Send to Environmental Protection Authority preferably by email ([email protected]) or alternatively by post (Private Bag 63002, Wellington 6140) Payment must accompany final application; see our fees and charges schedule for details.
Application Number
APP202506
Date
April 2015
www.epa.govt.nz 2
Application Form To obtain a determination of whether an organism is a new organism
Completing this application form
1. This form is used when you wish to apply for a statutory determination under section 26 of the Hazardous Substances and New Organisms (HSNO) Act 1996 as to whether or not an organism is a new organism (i.e. whether the organism is regulated under the HSNO Act or not). 2. If you wish to make an application for approval of for use of a new organism, a different form will have to be used. All forms are available on our website. 3. It is recommended that you contact an Advisor at the Environmental Protection Authority (EPA) as early in the application process as possible. An Advisor can assist you with any questions you have during the preparation of your application. 4. Unless otherwise indicated, all sections of this form must be completed for the application to be formally received and assessed. If a section is not relevant to your application, please provide a comprehensive explanation why this does not apply. If you choose not to provide the specific information, you will need to apply for a waiver under section 59(3)(a)(ii) of the HSNO Act. This can be done by completing the section on the last page of this form. 5. Any extra material that does not fit in the application form must be clearly labelled and cross- referenced, and included with the application form when it is submitted. 6. Please add extra rows/tables where needed. 7. You must sign the final form (the EPA will accept electronically signed forms) and pay the application fee (including GST) unless you are already an approved EPA customer. To be recognised by the EPA as an “approved customer”, you must have submitted more than one application per month over the preceding six months, and have no history of delay in making payments, at the time of presenting an application. 8. Information about application fees is available on the EPA website. 9. All application communications from the EPA will be provided electronically, unless you specifically request otherwise. Commercially sensitive information
10. Commercially sensitive information must be included in an appendix to this form and be identified as confidential. If you consider any information to be commercially sensitive, please show this in the relevant section of this form and cross reference to where that information is located in the confidential appendix. 11. Any information you supply to the EPA prior to formal lodgement of your application will not be publicly released. Following formal lodgement of your application any information in the body of this application form and any non-confidential appendices will become publicly available. 12. Once you have formally lodged your application with the EPA, any information you have supplied to the EPA about your application is subject to the Official Information Act 1982 (OIA). If a request is made for the release of information that you consider to be confidential, your view will be considered in a manner consistent with the OIA and with section 57 of the HSNO Act. You may be required to provide further justification for your claim of confidentiality.
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Application Form To obtain a determination of whether an organism is a new organism
Definitions
Any organism in which any of the genes or other genetic material: • Have been modified by in vitro techniques, or Genetically Modified • Are inherited or otherwise derived, through any number of replications, from Organism (GMO) any genes or other genetic material which has been modified by in vitro techniques
A new organism is an organism that is any of the following: • An organism belonging to a species that was not present in New Zealand immediately before 29 July 1998; • An organism belonging to a species, subspecies, infrasubspecies, variety, strain, or cultivar prescribed as a risk species, where that organism was not present in New Zealand at the time of promulgation of the relevant regulation; • An organism for which a containment approval has been given under the HSNO Act; • An organism for which a conditional release approval has been given under the HSNO Act; • New Organism A qualifying organism approved for release with controls under the HSNO Act; • A genetically modified organism; • An organism belonging to a species, subspecies, infrasubspecies, variety, strain, or cultivar that has been eradicated from New Zealand; • An organism present in New Zealand before 29 July 1998 in contravention of the Animals Act 1967 or the Plants Act 1970. This does not apply to the organism known as rabbit haemorrhagic disease virus, or rabbit calicivirus A new organism does not cease to be a new organism because: • It is subject to a conditional release approval; or • It is a qualifying organism approved for release with controls; or • It is an incidentally imported new organism
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Application Form To obtain a determination of whether an organism is a new organism
1. Applicant details
1.1. Applicant
Company Name: (if applicable) SafeTech Consulting Ltd.
Contact Name: Ajit Surjupersad
Job Title: Director
Physical Address: 38a Ashby Ave. St Heliers, Auckland
Postal Address (provide only if not the same as the physical):
Phone (office and/or mobile): + 64 21 708 752
Fax: n/a
Email: [email protected]
1.2. New Zealand agent or consultant (if applicable)
Company Name:
Contact Name:
Job Title:
Physical Address:
Postal Address (provide only if not the same as the physical):
Phone (office and/or mobile):
Fax:
Email:
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Application Form To obtain a determination of whether an organism is a new organism
2. Information about the organism
2.1. Name of organism Identify the organism as fully as possible
Organism name:
1. Bacillus psychrosaccharolyticus
Briefly describe the biological characteristics of the organism:
A facultative anaerobic Gram-positive psychrophilic/psychrotrophic bacterium1, (ex Larkin and Stokes, 1967) Priest et al., 1989
Current NZ status:
EPA: Not listed in any HSNO applications.
LandCare: Not Listed.
Organism name:
2. Bosea lupini
Briefly describe the biological characteristics of the organism:
Gram-negative, rod-shaped bacteria originally isolated from root nodules of Lupinus polyphyllus2 .
Current NZ status:
EPA: Not listed in any HSNO applications.
LandCare: Not Listed.
Organism name:
3. Duganella violaceinigra Synonym Pseudoduganella violaceinigra (Li et al. 2004) Kämpfer et al. 2012, comb. nov. Duganella violaceusniger Briefly describe the biological characteristics of the organism:
Gram-negative, short, rod-shaped, motile and non-spore-forming cells with flagella. Mesophilic, first described as an isolate from forest soil in Yunnan province, China 3.
Current NZ status:
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Application Form To obtain a determination of whether an organism is a new organism
EPA: Not listed in any HSNO applications. A closely related species Duganella radicus sp. nov. Madhaiyan was approved with controls NOC99023.
LandCare: Not listed. A closely related species Duganella zoogloeoides is listed as present in New Zealand (http://nzfungi2.landcareresearch.co.nz).
Organism name:
4. Flavobacterium glaciei 5. Flavobacterium saccharophilum
Briefly describe the biological characteristics of the organism:
• Flavobacterium glaciei Gram-negative psychrophilic, yellow-pigmented and obligate aerobic bacterium, first described as an isolate from the China No.1 glacier4.
• Flavobacterium saccharophilum Gram-negative, previously known as Cytophaga saccharophila, first described as an isolate from freshwater (Reichenbach 1989) Bernardet et al. 1996, comb. nov.
Current NZ status:
EPA: Not listed in any HSNO applications.
LandCare: A closely related species Flavobacterium columnare is listed as present in New Zealand (http://nzfungi2.landcareresearch.co.nz).
Organism name:
6. Massilia niastensis
Briefly describe the biological characteristics of the organism:
Gram negative, aerobic, motile, rod-shaped bacterium originally described as an isolate from air samples in Suwon, Korea5.
Current NZ status:
EPA: Not listed in any HSNO applications.
LandCare: Not listed
Organism name:
7. Pseudomonas abietaniphila 8. Pseudomonas psychrotolerans
Briefly describe the biological characteristics of the organism:
• Pseudomonas abietaniphila
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Application Form To obtain a determination of whether an organism is a new organism
Gram negative soil bacterium isolated from pulp mill effluent6
• Pseudomonas psychrotolerans Yellow-pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium7
Current NZ status:
EPA: Not listed in any HSNO applications. A number of closely related Pseudomonas sp. are determined as present in NZ.
LandCare: P. abietaniphila and P. psychrotolerans are both listed as present/isolated in New Zealand after 1996 (http://nzfungi2.landcareresearch.co.nz).
Organism name:
9. Rhizobacter fulvus Synonym Methylibium fulvum
Briefly describe the biological characteristics of the organism:
Gram-negative, motile, aerobic rod-shaped bacterium previously known as Methylibium fulvum.
Current NZ status:
EPA: Closely related species Rhizobacter daucus - Approved with controls (NOC001909)
LandCare: Not listed
Organism name:
10. Sphingobium quisquiliarum 11. Sphingobium yanoikuyae
Briefly describe the biological characteristics of the organism:
• Sphingobium quisquiliarum Yellow-pigmented, Gram-negative, hexachlorocyclohexane (HCH)-degrading bacterium; type strain isolated from an HCH dump site in the northern part of India8
• Sphingobium yanoikuyae Yellow-pigmented, Gram-negative bacterium previously known as Sphingomonas yanoikuyae9
Current NZ status:
EPA: • Sphingobium yanoikuyae determined not present in NZ (PNZ1000024), Sphingobium scionense determined present in NZ (PNZ1000140),
LandCare: A closely related species Sphingobium scionense was isolated in NZ.
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Application Form To obtain a determination of whether an organism is a new organism
Organism name:
12. Albidiferax ferrireducens synonym Rhodoferax ferrireducens Briefly describe the biological characteristics of the organism:
Cells are Gram-negative, short rods, 3–5 mm long by 1 mm wide, that are motile via a single polar flagellum. Colonies are glossy white, smooth, round and convex. Optimum temperature and pH are 25 uC and 7?0, respectively. Grows at and reduces Fe(III) at temperatures as low as 4 uC. There is no fermentative or phototrophic growth. Facultatively anaerobic: respires with Fe(III)–NTA, Mn(IV) oxide, fumarate, nitrate and atmospheric oxygen10.
Current NZ status:
EPA: Not listed in any HSNO applications.
LandCare: Not listed in Landcare database.
Organism name:
13. Caulobacter henricii Briefly describe the biological characteristics of the organism:
Bright yellow colonies. Gram negative vibroid Alpha proteobacteria11 (Poindexter 1964).
Current NZ status:
EPA: Not listed in any HSNO applications.
LandCare: Isolate from pasture rhizosphere from Winchmore, Canterbury, 2010.
Organism name:
14. Polaromonas ginsengisoli Briefly describe the biological characteristics of the organism:
Gram negative Betaproteobacteria.
Current NZ status:
EPA: Not listed in any HSNO applications.
LandCare: Not listed in Landcare database.
Organism name:
15. Mucilaginibacter dorajii Briefly describe the biological characteristics of the organism:
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Application Form To obtain a determination of whether an organism is a new organism
Cells are Gram-negative, strictly aerobic, catalase-positive, oxidase-positive and nonmotile rods (measuring 1.1–1.8 X 0.6–0.8 µm). Flexirubin-type pigment is present. Colonies are circular, smooth, mucoid, convex and entire, and the colony color is light yellow. The temperature range for growth is between 4 1C and 301C (optimally at 20–25 1C). The initial media pH range for growth is pH 5.0–8.0; the optimal pH was 5.5–6.0. Growth occurs in the presence of 0–1% NaCl, but not over 2% NaCl12 (Kim et al, 2010).
Current NZ status:
EPA: Closely related species, Mucilaginibacter gossypii is present in NZ (APP202376)
LandCare: Not listed in Landcare database.
Organism name:
16. Duganella zoogloeoides synonym Zoogloea ramigera Briefly describe the biological characteristics of the organism:
Cells are gram-negative, non-spore-forming, motile rods. Cells are straight or slightly curved rods that are 0.6 to 0.8 µm wide and 1.8 to 3.0 µm long. Motile by means of single polar flagella. Colonies on nutrient agar media are glistening, convex with entire margins, viscous, and pale yellow to straw coloured. Tough, leathery, dry, wrinkled colonies appear in some cases. Aerobic chemoorganotrophs having a strictly respiratory type of metabolism with oxygen as the terminal electron acceptor13 (Hiraishi et al, 1997).
Current NZ status:
EPA: Not listed in any HSNO applications.
LandCare: Present in NZ, isolate from pasture rhizosphere sampled from Winchmore, Canterbury, 2010.
Organism name:
17. Herbaspirillum huttiense synonym Pseudomonas huttiense Briefly describe the biological characteristics of the organism:
Gram-negative rods with blunt ends averaging 0.4 by 18 µm. The soma has a small but distinct curvature. Within the soma are generally visible two to three round inclusions about 0.3 µm in diameter. The organisms are usually single. Motile with polar flagella, frequently bipolar. The number of flagella varies from one to three and the organism may be referred to as multitrichous. Colonies on agar are smooth, colorless and opaque. Growth on slants is smooth and colourless14 (Leifson, 1962).
Current NZ status:
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Application Form To obtain a determination of whether an organism is a new organism
EPA: Not listed in any HSNO applications.
LandCare: Present in NZ, isolate from sputum, as well as soil and associated plant material sampled at Napier, June 2009.
Organism name:
18. Stenotrophomonas rhizophila 19. Stenotrophomonas chelatiphaga Briefly describe the biological characteristics of the organism:
• Stenotrophomonas rhizophila Straight or slightly curved rods. Colonies are yellowish; the colour is not due to carotenoid pigments or to xanthomonadins. Growth takes place at 4±37°C but not at 40°C. Neither lipolytic nor β-glucosidase activity occurs. The strains use xylose as a carbon source. Strains were plant-associated and isolated from the rhizosphere of oilseed rape and from the the rhizosphere and geocaulsophere (tuber) of potato. Endophytic colonization was found15.
• Stenotrophomonas chelatiphaga Gram-negative rods, 0.5 x 0.8–1.3 µm in size, occurred singly or in pairs, and multiplied by binary fission. Cells are motile by a single polar flagellum. Colonies are white on mineral salts/EDTA agar (0.1–0.3mm in diameter) and yellowish on trypticase soy agar (2–3mm in diameter). Able to grow at 25–40°C, at pH 5.5–9.5 and optimally at 28– 37°C, at pH 6.5–8.5. Catalase-, oxidase- and β-galactosidase-positive but urease and lipase-negative16.
Current NZ status:
EPA: The closely related species, Stenotrophomonas maltophilia is considered present in NZ (BER00001).
LandCare: Stenotrophomonas rhizophilia is present in NZ, with isolates from soil and associated plant material in Napier, Northland and Wellington on June 2008.
2.2. Regulatory status of the organism
Is the organism that is the subject of this application also the subject of:
An innovative medicine application as defined in section 23A of the Medicines Act 1981?
☐ Yes ☒ No
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Application Form To obtain a determination of whether an organism is a new organism
An innovative agricultural compound application as defined in Part 6 of the Agricultural Compounds and Veterinary Medicines Act 1997?
☐ Yes ☒ No
3. Evidence regarding whether the organism meets the definition of a new organism
Does the organism meet the definition of a new organism under the HSNO Act?
For example, does it belong to a species that was not present in New Zealand before July 29 1998? Is it a genetically modified organism? etc.
Describe the evidence you have to support this view, providing supporting materials in an appendix as appropriate. 1. Bacillus psychrosaccharolyticus
In New Zealand, at least 10 isolates identified to be B. psychrosaccharolyticus by 16S rRNA gene sequence 17 have been obtained from 9 samples (mostly soil) collected from locations spanning Auckland to Kapiti between April 2013 and May 2014. BLAST alignment 18 of 16S rRNA gene sequences from these isolates showed 99.6% to 99.9% (mean 99.8%) identity to the type strain of B. psychrosaccharolyticus over an average of 937 base pairs (range 725 to 1078 base pairs).
Blast identity Isolate Blast hit (%) Sample site Sample date 1 Bacillus psychrosaccharolyticus strain NBRC 101233 99.9% Pokaka, National Park Apr-13 2 Bacillus psychrosaccharolyticus strain NBRC 101233 99.9% Otaki, Kapiti Jan-14 3 Bacillus psychrosaccharolyticus strain NBRC 101233 99.9% Otaki, Kapiti Jan-14 4 Bacillus psychrosaccharolyticus strain NBRC 101233 99.6% Raumati, Kapiti Jan-14 5 Bacillus psychrosaccharolyticus strain NBRC 101233 99.8% Raumati, Kapiti Jan-14 6 Bacillus psychrosaccharolyticus strain NBRC 101233 99.9% Otaki, Kapiti Jan-14 7 Bacillus psychrosaccharolyticus strain NBRC 101233 99.9% Lower Kaimai,BOP May-14 8 Bacillus psychrosaccharolyticus strain NBRC 101233 99.9% Lower Kaimai,BOP May-14 9 Bacillus psychrosaccharolyticus strain NBRC 101233 99.8% Glenfield, Auckland May-14 10 Bacillus psychrosaccharolyticus strain NBRC 101233 99.8% Meadowbank, Auckland May-14
All B. psychrosaccharolyticus isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
The original isolates of B. psychrosaccharolyticus were obtained from soil, mud and water from lowland fields, streams and lakes in the 1960s (Larkin & Stokes, 1966, 1967). Since
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Application Form To obtain a determination of whether an organism is a new organism then, the bacterium has also been isolated from sediments, soil and water/ice samples from lakes, mountains and cold deserts, (Sahay et al., 2013; Yadav et al., 2015, unpublished Genbank KJ589486), as well as the rhizosphere of yellow-tuft (Alyssum murale) (Abou-Shanab et al., 2010; Abou-Shanab et al., 2003).
Considering the global distribution of B. psychrosaccharolyticus and the ability to readily isolate the bacterium in New Zealand, it is highly likely that B. psychrosaccharolyticus is ubiquitous wherever suitable environmental conditions exist within New Zealand and is not a new organism.
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Application Form To obtain a determination of whether an organism is a new organism
2. Bosea lupini
[See summary table for evidence supporting ubiquity]
In New Zealand, at least 10 isolates of B. lupini, classified using RDP Sequence Match 17, have been isolated from 9 samples (mostly soils) taken from sites spanning Northland to Kapiti between August 2012 to May 2014. BLAST alignment (Zhang, Schwartz, Wagner, & Miller, 2000) of the 16S rRNA gene sequences showed these 10 isolates had between 99.0% to 99.9% (average 99.7%) identity to known B. lupini strains over an average of 1029 base pairs (range 813 to 1088 base pairs).
Blast identity Sample Isolate Blast hit (%) Sample site date 1 Bosea lupini 99.8 Mt Albert, Auckland May-14 2 Bosea lupini 99.8 Mt Albert, Auckland May-14 3 Bosea lupini 99.7 Mt Albert, Auckland May-14 4 Bosea lupini 99.9 Meadowbank, Auckland May-14 5 Bosea lupini 99.8 Papatoetoe, Auckland May-14 6 Bosea lupini 99 Raumati, Kaptiti May-14 7 Bosea lupini 99.8 Raumati, Kaptiti May-14 8 Bosea lupini 99.7 Hukatere , Northland Aug-12 9 Bosea lupini 99.8 Pokaka, nr National Park May-14 10 Bosea lupini 99.9 Lower Kaimai, BOP May-14
All B. lupini isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Considering the global distribution of lupine (a plant host for B. lupini) and its long history of naturalisation in NZ, as well as the ability to readily isolate the bacterium in New Zealand, it is highly likely that B. lupini is ubiquitous wherever suitable environmental conditions exist within New Zealand and is not a new organism.
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Application Form To obtain a determination of whether an organism is a new organism
3. Duganella violaceinigra
Synonyms Pseudoduganella violaceinigra or Duganella violaceusniger
[See summary table for evidence supporting ubiquity]
In New Zealand, at least 25 Duganella violaceinigra isolates have been obtained from 21 samples (mostly soil samples) collected at locations spanning the North Island between September 2008 and May 2014. The isolates were classified as D. violaceinigra on the basis of the most closely related 16S rRNA gene sequences according to the RDP Sequence Match algorithm 17. BLAST alignment 18 of the 16S rRNA gene sequences demonstrated that these 25 isolates had between 97.5% to 100% (average 99.0%) sequence identity to known D. violaceinigra strains over an average of 874 base pairs (range 408 to 1090 base pairs).
Blast identity Sample Isolate Blast hit (%) Sample site date 1 Duganella violaceusniger isolate CP177-4 97.8 Paparoa, Northland Sep-08 2 Duganella violaceusniger isolate CP177-4 97.7 Paparoa, Northland Sep-08 3 Duganella violaceusniger isolate CP177-4 97.9 Pokaka, National Park Sep-08 4 Duganella violaceusniger isolate CP177-4 98 Napier Sep-08 5 Duganella violaceinigra strain YIM 31327 99.1 Bethells Beach Apr-13 6 Duganella violaceinigra strain R1545 99.4 Pokaka, National Park Apr-13 7 Duganella violaceinigra strain YIM 31327 99 Pokaka, National Park Apr-13 8 Duganella violaceinigra strain R1545 99.5 Pokaka, National Park Apr-13 9 Duganella violaceinigra strain YIM 31327 99.4 Pokaka, National Park Apr-13 10 Duganella violaceusniger isolate CP177-4 100 Hukatere, Northland Aug-12 11 Duganella violaceinigra strain YIM 31327 99.4 Raumati, Kapiti Aug-12 12 Duganella violaceinigra strain YIM 31327 99 Raumati, Kapiti Aug-12 13 Duganella violaceinigra strain YIM 31327 98.2 Pokaka, National Park Aug-12 14 Duganella violaceinigra strain R1545 97.5 Hukatere, Northland Aug-12 15 Duganella violaceinigra strain YIM 31327 99.2 Otaki, Kapiti Jan-14 16 Duganella violaceinigra strain YIM 31327 99.4 Otaki, Kapiti Jan-14 17 Duganella violaceinigra strain YIM 31327 99.2 Otaki, Kapiti Jan-14 18 Duganella violaceinigra strain YIM 31327 99.3 Otaki, Kapiti Jan-14 19 Duganella violaceinigra strain YIM 31327 99.2 Otaki, Kapiti Jan-14 20 Duganella violaceinigra strain YIM 31327 99.1 Bethells Beach Jan-14 21 Duganella violaceinigra strain YIM 31327 99.2 Waerenga, Waikato Jun-14 22 Duganella violaceinigra strain YIM 31327 99.6 Mt Albert, Auckland May-14 23 Duganella violaceinigra strain YIM 31327 99.3 Lower Kaimai, BOP May-14 24 Duganella violaceinigra strain YIM 31327 99.9 Hukatere, Northland May-14 25 Duganella violaceinigra strain YIM 31327 99.9 Lower Kaimai, BOP May-14
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Application Form To obtain a determination of whether an organism is a new organism
All D. violaceinigra isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Given the global distribution across diverse habitats, wide host range, documented ability to disperse large distances on intercontinental dust events, and the ability to readily isolate the bacterium in NZ, it is highly likely that D. violaceinigra is ubiquitous wherever suitable environmental conditions exist within New Zealand and are not new organisms.
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Application Form To obtain a determination of whether an organism is a new organism
4. Flavobacterium glaciei 5. Flavobacterium saccharophilum
[See summary table for evidence supporting ubiquity]
• Flavobacterium glaciei
At least 5 isolates of Flavobacterium glaciei, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 5 samples (mostly soil) collected from sites spanning BOP to Kapiti between August 2011 and May 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these 5 isolates had between 98.6% to 100% (mean 99.1%) identity to the type strain, or other known strains, over an average alignment of 829 bases (range 521 to 1279 bases).
Blast Sample Isolate Blast hit identity (%) Sample site date 1 Flavobacterium glaciei strain 0499 (T) 98.6 Otaki, Kapiti Jan-14 2 Flavobacterium glaciei strain EA2-2 100 Raumati, Kapiti Aug-11 3 Flavobacterium glaciei strain EA2-2 98.9 Lower Kaimai, BOP May-14 4 Flavobacterium glaciei strain EA2-2 98.9 Lower Kaimai, BOP May-14 5 Flavobacterium glaciei strain EA2-2 98.9 Lower Kaimai, BOP May-14
• Flavobacterium saccharophilum synonym Cytophaga saccharophila
At least 7 isolates of Flavobacterium saccharophilum, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 7 samples (mostly soil) collected from sites spanning Pokaka to Northland between August 2012 and May 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these 7 isolates had between 98.6% to 100% (mean 99.1%) identity to known strains of F. saccharophilum over an average alignment of 998 bases (range 758 to 1101 bases).
Blast Sample Isolate Blast hit identity (%) Sample site date 1 Flavobacterium saccharophilum NBRC 15944 99.4 Pokaka, near National Park Apr-13 2 Flavobacterium saccharophilum NBRC 15944 99.4 Hukatere, Northland Aug-12 3 Flavobacterium saccharophilum NBRC 15944 98.6 Hukatere, Northland Aug-12 4 Flavobacterium saccharophilum NBRC 15944 98.7 Hukatere, Northland Aug-12 5 Flavobacterium saccharophilum NBRC 15944 99.1 Pokaka, near National Park Mar-13 6 Flavobacterium saccharophilum NBRC 15944 99.9 Lower Kaimai, BOP May-14 7 Flavobacterium saccharophilum NBRC 15944 98.8 Meadowbank, Auckland May-14
All F. saccharophilum and F. glaciei isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Given the global distribution in a wide range of habitats and the ability to readily isolate the bacterium in NZ, it is highly likely that F. saccharophilum and F. glaciei are ubiquitous wherever suitable environmental conditions exist within New Zealand and are not new organisms.
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Application Form To obtain a determination of whether an organism is a new organism
6. Massilia niastensis
[See summary table for evidence supporting ubiquity]
At least 43 isolates of Massilia niastensis, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 36 samples (mostly soil) collected from multiple sites spanning the North Island between August 2011 and May 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these 43 isolates had between 98.4% to 100% (mean 99.1%) identity to published strains over an average alignment of 890 bases (range 408 to 1114 bases).
Blast identity Isolate Blast hit (%) Sample site Sample date 1 Massilia niastensis strain 5516S-1 98.2 Napier Aug-11 2 Massilia niastensis strain 5516S-1 99 Hukatere, Northland Aug-12 3 Massilia niastensis strain 5516S-1 100 Hukatere, Northland Aug-12 4 Massilia niastensis strain 5516S-1 99.4 Pukekohe, Auckland Apr-13 5 Massilia niastensis strain 5516S-1 98.5 Pokaka, nr National Park Apr-13 6 Massilia niastensis strain 5516S-1 98.1 Bethells Beach Apr-13 7 Massilia niastensis strain 5516S-1 98.9 Otaki, Kapiti Dec-13 8 Massilia niastensis strain A6-12 100 Manakau, Kapiti Aug-11 9 Massilia niastensis strain A6-12 100 Pokaka, nr National Park Apr-13 10 Massilia niastensis strain GHM816 98.8 Hukatere , Northland Aug-12 11 Massilia niastensis strain GHM816 98.9 Otaki, Kapiti Dec-13 12 Massilia niastensis strain WA6-3 98.6 Raumati, Kapiti Aug-11 13 Massilia niastensis strain WA6-3 99.5 Raumati, Kapiti Aug-11 14 Massilia niastensis strain WA6-3 98.3 Hukatere, Northland Aug-11 15 Massilia niastensis strain WA6-3 98.7 Hukatere , Northland Aug-12 16 Massilia niastensis strain WA6-3 99.3 Pokaka, nr National Park Apr-13 17 Massilia niastensis strain WA6-3 99.2 Pokaka, nr National Park Apr-13 18 Massilia niastensis strain WA6-3 98.4 Raumati, Kapiti Apr-13 19 Massilia niastensis strain WA6-3 98.9 Raumati, Kapiti Apr-13 20 Massilia niastensis strain WA6-3 98.5 Raumati, Kapiti Apr-13 21 Massilia niastensis strain WA6-3 98.8 Raumati, Kapiti Apr-13 22 Massilia niastensis strain WA6-3 99.5 Raumati, Kapiti Apr-13 23 Massilia niastensis strain WA6-3 99.4 Pokaka, nr National Park May-14 24 Massilia niastensis strain WA6-3 98.5 Pokaka, nr National Park Apr-13 25 Massilia niastensis strain WA6-3 99 Pokaka, nr National Park Apr-13 26 Massilia niastensis strain WA6-3 99.4 Pokaka, nr National Park Apr-13 27 Massilia niastensis strain WA6-3 99.4 Pokaka, nr National Park Apr-13 28 Massilia niastensis strain WA6-3 99.4 Pokaka, nr National Park Apr-13 29 Massilia niastensis strain WA6-3 99.5 Pokaka, nr National Park Apr-13 30 Massilia niastensis strain WA6-3 98.1 Bethells Beach Apr-13 31 Massilia niastensis strain WA6-3 98.7 Bethells Beach Apr-13
December 2013 EPA0327 18
Application Form To obtain a determination of whether an organism is a new organism
32 Massilia niastensis strain WA6-3 99.4 Bethells Beach Apr-13 33 Massilia niastensis strain WA6-3 99.3 Otaki, Kapiti Dec-13 34 Massilia niastensis strain WA6-3 99.6 Otaki, Kapiti Dec-13 35 Massilia niastensis strain WA6-3 99.6 Otaki, Kapiti Dec-13 36 Massilia niastensis strain WA6-3 99.7 Otaki, Kapiti Dec-13 37 Massilia niastensis strain WA6-3 99.5 Otaki, Kapiti May-14 38 Massilia niastensis strain WA6-3 98.6 Lower Kaimai, BOP May-14 39 Massilia niastensis strain WA6-3 98.8 Mt Albert, Auckland May-14 40 Massilia niastensis strain WA6-3 99.4 Mt Albert, Auckland May-14 41 Massilia niastensis strain WA6-3 99.2 Papatoetoe, Auckland May-14 42 Massilia niastensis strain WA6-3 99.2 Raumati, Kapiti May-14 43 Massilia niastensis strain XA4-8 99.3 Hukatere , Northland Aug-12
All M. niastensis isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Considering the global distribution of M. niastensis across a wide range of habitats, its association with a plant host with a long history in NZ, and the ability to readily isolate the bacterium in New Zealand, it is highly likely that M. niastensis is ubiquitous wherever suitable environmental conditions exist within New Zealand and is not a new organism.
December 2013 EPA0327 19
Application Form To obtain a determination of whether an organism is a new organism
7. Pseudomonas abietaniphila 8. Pseudomonas psychrotolerans
[See summary table for evidence supporting ubiquity]
• Pseudomonas abietaniphila
At least 7 isolates of P. abietaniphila, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 6 samples (mostly soil) collected from sites spanning Northland to Kapiti between August 2011 and January 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these 7 isolates had between 99.9% to 100% (mean 99.7%) identity to the type strain over an average alignment of 927 bases (range 719 to 1044 bases).
Blast identity Isolate Blast hit (%) Sample site Sample date 1 Pseudomonas abietaniphila strain BKME-9 99 Napier Aug-11 2 Pseudomonas abietaniphila strain BKME-9 100 Raumati, Kapiti Aug-12 3 Pseudomonas abietaniphila strain BKME-9 100 Raumati, Kapiti Aug-12 4 Pseudomonas abietaniphila strain BKME-9 99.7 Hukatere, Northland Aug-12 5 Pseudomonas abietaniphila strain BKME-9 99.9 Hukatere, Northland Aug-12 6 Pseudomonas abietaniphila strain BKME-9 99.7 Otaki, Kapiti Jan-14 7 Pseudomonas abietaniphila strain BKME-9 99.9 Otaki, Kapiti Jan-14
• Pseudomonas psychrotolerans
At least 20 isolates of P. psychrotolerans, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 12 samples (mostly soil) collected from sites spanning Kapiti to Northland between August 2011 and January 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these 20 isolates had between 98.8% to 100% (mean 99.7%) identity to the type strain or other published strains over an average alignment of 945 bases (range 615 to 1109 bases).
Blast Sample Isolate Blast hit identity (%) Sample site date 1 Pseudomonas psychrotolerans C36 (T) 100 Otaki, Kapiti Jan-14 2 Pseudomonas psychrotolerans C36 (T) 99.9 Raumati, Kapiti Aug-12 3 Pseudomonas psychrotolerans C36 (T) 99.3 Raumati, Kapiti Aug-12 4 Pseudomonas psychrotolerans C36 (T) 100 Raumati, Kapiti Apr-13 5 Pseudomonas psychrotolerans C36 (T) 100 Raumati, Kapiti Apr-13 6 Pseudomonas psychrotolerans C36 (T) 100 Pokaka, National Park Apr-13 7 Pseudomonas psychrotolerans C36 (T) 100 Pokaka, National Park Jan-14 8 Pseudomonas psychrotolerans strain M38 100 Raumati, Kapiti Aug-12 9 Pseudomonas psychrotolerans strain M38 100 Raumati, Kapiti Aug-12 10 Pseudomonas psychrotolerans strain M38 99.1 Raumati, Kapiti Aug-12 11 Pseudomonas psychrotolerans strain M38 98.7 Raumati, Kapiti Aug-12 12 Pseudomonas psychrotolerans strain M38 98.8 Raumati, Kapiti Aug-12
December 2013 EPA0327 20
Application Form To obtain a determination of whether an organism is a new organism
13 Pseudomonas psychrotolerans strain M38 99.5 Pokaka, National Park Aug-12 14 Pseudomonas psychrotolerans strain M38 100 Raumati, Kapiti Apr-13 15 Pseudomonas psychrotolerans strain M38 99.8 Raumati, Kapiti Apr-13 16 Pseudomonas psychrotolerans strain M38 100 Raumati, Kapiti Apr-13 17 Pseudomonas psychrotolerans strain M38 100 Raumati, Kapiti Apr-13 18 Pseudomonas psychrotolerans S8-435 16S 99.8 Hukatere, Northland Aug-11 19 Pseudomonas psychrotolerans strain -Y42 99.8 Hukatere, Northland Aug-12 20 Pseudomonas psychrotolerans strain -Y42 100 Auckland Feb-13
All P. abietaniphila and P. psychrotolerans isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Given the global distribution across diverse habitats, wide host range in plant hosts that are found in NZ, and the ability to readily isolate the bacterium in NZ, it is highly likely that P. abietaniphila and P. psychrotolerans are ubiquitous wherever suitable environmental conditions exist within New Zealand and are not new organisms.
December 2013 EPA0327 21
Application Form To obtain a determination of whether an organism is a new organism
9. Rhizobacter fulvus (Synonym Methylibium fulvum)
[See summary table for evidence supporting ubiquity]
At least 13 isolates of Rhizobacter fulvus, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 9 samples (mostly soil) collected from multiple sites spanning the North Island between August 2012 and January 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these 13 isolates had between 98.6% to 100% (mean 99.0%) identity to the type strain and other published strains over an average alignment of 856 bases (range 558 to 1102 bases).
Blast Sample Isolate Blast hit identity (%) Sample site date 1 Methylibium fulvum Gsoil 328 99.5 Pokaka, National Park Aug-12 2 Methylibium fulvum Gsoil 328 97.8 Hukatere Northland Apr-13 3 Methylibium fulvum Gsoil 328 99.3 Hukatere Northland Apr-13 4 Methylibium fulvum Gsoil 328 100.0 Hukatere Northland Apr-13 5 Methylibium fulvum S32403 99.3 Hukatere Northland Apr-13 6 Rhizobacter fulvus strain Gsoil 322 (T) 99.6 Hukatere Northland Aug-12 7 Rhizobacter fulvus strain Gsoil 322 (T) 99.7 Hukatere Northland Apr-13 8 Rhizobacter fulvus strain Gsoil 322 (T) 100.0 Otaki, Kapiti Jan-14 9 Rhizobacter fulvus strain Gsoil 322 (T) 98.7 Otaki, Kapiti Jan-14 10 Rhizobacter fulvus strain Gsoil 322 (T) 98.8 Otaki, Kapiti Jan-14 11 Rhizobacter fulvus strain Gsoil 322 (T) 98.8 Otaki, Kapiti Jan-14 12 Rhizobacter fulvus strain Gsoil 322 (T) 97.0 Bethells Beach Jan-14 13 Rhizobacter fulvus strain Gsoil 322 (T) 98.6 Raumati, Kapiti Jan-14
All R. fulvus isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Considering the global distribution of R. fulvus across a range of habitats, the presence of its animal and plant hosts in NZ, and the ability to readily isolate the bacterium in New Zealand, it is highly likely that R. fulvus is ubiquitous wherever suitable environmental conditions exist within New Zealand and is not a new organism.
December 2013 EPA0327 22
Application Form To obtain a determination of whether an organism is a new organism
10. Sphingobium quisquiliarum 11. Sphingobium yanoikuyae
[Ubiquity background info to be completed by EPA and applicant as required]
• Sphingobium quisquiliarum
At least 6 isolates of S. quisquiliarum, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 4 samples (mostly soil) collected from sites spanning Kapiti to Northland between August 2012 and January 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these 6 isolates had between 98.8% to 99.0% (mean 99.2%) identity to the type strain over an average alignment of 1095 bases (range 1034 to 1286 bases).
Blast Sample Isolate Blast hit identity (%) Sample site date 1 Sphingobium quisquiliarum strain P25 98.8 Hukatere, Northland Aug-12 2 Sphingobium quisquiliarum strain P25 99.2 Hukatere, Northland Aug-12 3 Sphingobium quisquiliarum strain P25 99.1 Hukatere, Northland Aug-12 4 Sphingobium quisquiliarum strain P25 99.4 Raumati, Kapiti Apr-13 5 Sphingobium quisquiliarum strain P25 99 Raumati, Kapiti May-13 6 Sphingobium quisquiliarum strain P25 99.5 Otaki, Kapiti Jan-14
• Sphingobium yanoikuyae
At least 21 isolates of S. yanoikuyae, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 14 samples (mostly soil) collected from sites spanning Kapiti to Northland between August 2011 and January 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these 21 isolates had between 97.1% to 100% (mean 99.5%) identity to published strains over an average alignment of 927 bases (range 600 to 1087 bases).
Blast Sample Isolate Blast hit identity (%) Sample site date 1 Sphingobium yanoikuyae strain CH-Y-1 99.7 Paparoa, Kaipara Aug-08 2 Sphingobium yanoikuyae strain KUDC1818 99 Pokaka, National Park Feb-13 3 Sphingobium yanoikuyae strain NBRC 15102 99.7 Raumati, Kapiti Aug-11 4 Sphingobium yanoikuyae strain NBRC 15102 99.7 Raumati, Kapiti Aug-11 5 Sphingobium yanoikuyae strain NBRC 15102 100 Raumati, Kapiti Apr-13 6 Sphingobium yanoikuyae strain NBRC 15102 100 Otaki, Kapiti Dec-13 7 Sphingobium yanoikuyae strain NBRC 15102 100 Otaki, Kapiti Dec-13 8 Sphingobium yanoikuyae strain NBRC 15102 99.5 Otaki, Kapiti Dec-13 9 Sphingobium yanoikuyae strain NBRC 15102 100 Otaki, Kapiti Dec-13 10 Sphingobium yanoikuyae strain NBRC 15102 99.9 Otaki, Kapiti Dec-13 11 Sphingobium yanoikuyae strain NBRC 15102 99.9 Otaki, Kapiti Dec-13 12 Sphingobium yanoikuyae strain NBRC 15102 100 Bethells Beach May-14 13 Sphingobium yanoikuyae strain NBRC 15102 97.1 Kauri Point, Katikati May-14 14 Sphingomonas yanoikuyae Q1 97.5 Titirangi, Auckland Mar-10 15 Sphingomonas yanoikuyae Q1 98.6 Raumati, Kapiti Aug-11
December 2013 EPA0327 23
Application Form To obtain a determination of whether an organism is a new organism
16 Sphingomonas yanoikuyae Q1 99.9 Raumati, Kapiti Aug-12 17 Sphingomonas yanoikuyae Q1 99.9 Raumati, Kapiti Aug-12 18 Sphingomonas yanoikuyae Q1 99.9 Raumati, Kapiti Aug-12 19 Sphingomonas yanoikuyae Q1 99.4 Hukatere, Northland Aug-12 20 Sphingomonas yanoikuyae Q1 99.8 Lynfield, Auckland Feb-13 21 Sphingomonas yanoikuyae Q1 100 Otaki, Kapiti Dec-13
All S. quisquiliarum and S. yanoikuyae isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Given the global distribution across diverse habitats, wide host range and the ability to readily isolate the bacterium in NZ, it is highly likely that S. quisquiliarum and S. yanoikuyae are ubiquitous wherever suitable environmental conditions exist within New Zealand and are not new organisms.
December 2013 EPA0327 24
Application Form To obtain a determination of whether an organism is a new organism
12. Albidiferax ferrireducens (synonym Rhodoferax ferrireducens)
[See summary table for evidence supporting ubiquity]
At least 11 isolates of Albidiferax ferrireducens, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 8 samples (mostly soil) collected from multiple sites spanning the Northland to Kapiti between August 2012 and May 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these 11 isolates had between 98.8% to 99.6% (mean 99.1%) identity to the type strain over an average alignment of 956 bases (range 740 to 1102 bases).
Blast Sample Isolate Blast hit identity (%) Sample site date 1 Albidiferax ferrireducens strain PAMC 25181 99.3 Hukatere, Northland Aug-12 2 Albidiferax ferrireducens strain PAMC 25181 98.8 Hukatere, Northland Aug-12 3 Albidiferax ferrireducens strain PAMC 25181 98.9 Hukatere, Northland Aug-12 4 Albidiferax ferrireducens strain PAMC 25181 98.8 Hukatere, Northland Apr-13 5 Albidiferax ferrireducens strain PAMC 25181 99.3 Otaki, Kapiti Jan-14 6 Albidiferax ferrireducens strain PAMC 25181 98.8 Otaki, Kapiti Jan-14 7 Albidiferax ferrireducens strain PAMC 25181 98.8 Otaki, Kapiti Jan-14 8 Albidiferax ferrireducens strain PAMC 25181 98.8 Mt Albert, Auckland May-14 9 Albidiferax ferrireducens strain PAMC 25181 99.5 Raumati, Kapiti May-14 10 Albidiferax ferrireducens strain PAMC 25181 99.5 Meadowbank, Auckland May-14 11 Albidiferax ferrireducens strain PAMC 25181 99.6 Meadowbank, Auckland May-14
All A. ferrireducens isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Considering the global distribution of A. ferrireducens across diverse habitats and the ability to readily isolate the bacterium in New Zealand, it is highly likely that A. ferrireducens is ubiquitous wherever suitable environmental conditions exist within New Zealand and is not a new organism.
December 2013 EPA0327 25
Application Form To obtain a determination of whether an organism is a new organism
13. Caulobacter henricii
[See summary table for evidence supporting ubiquity]
At least 25 isolates of Caulobacter henricii, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 21 samples (mostly soil) collected from multiple sites spanning the North Island between August 2012 and May 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these 25 isolates had between 97% to 100% (mean 99.0%) identity to known strains over an average alignment of 852 bases (range 356 to 1098 bases).
Blast Isolate Blast hit identity (%) Sample site Sample date 1 Caulobacter henricii strain ATCC 15253 99.3 Raumati, Kapiti Aug-12 2 Caulobacter henricii strain ATCC 15253 98.5 Hukatere, Northland Aug-12 3 Caulobacter henricii strain ATCC 15253 97.0 Hukatere, Northland Aug-12 4 Caulobacter henricii strain ATCC 15253 99.2 Hukatere, Northland Aug-12 5 Caulobacter henricii strain ATCC 15253 99.2 Pukekohe Apr-13 6 Caulobacter henricii strain ATCC 15253 99.5 Pokaka, near National Park Apr-13 7 Caulobacter henricii strain ATCC 15253 99.3 Raumati, Kapiti Apr-13 8 Caulobacter henricii isolate PhyCEm-80 99.0 Raumati, Kapiti Apr-13 9 Caulobacter henricii isolate PhyCEm-80 99.2 Hukatere, Northland Apr-13 10 Caulobacter henricii isolate PhyCEm-80 99.0 Hukatere, Northland Apr-13 11 Caulobacter henricii isolate PhyCEm-80 100.0 Hukatere, Northland Apr-13 12 Caulobacter henricii isolate PhyCEm-80 99.4 Otaki, Kapiti Jan-14 13 Caulobacter henricii isolate PhyCEm-80 99.4 Raumati, Kapiti Jan-14 14 Caulobacter henricii strain ATCC 15253 98.3 Otaki, Kapiti Jan-14 15 Caulobacter henricii isolate PhyCEm-80 97.0 Katikati, BOP May-14 16 Caulobacter henricii isolate PhyCEm-80 99.3 Katikati, BOP May-14 17 Caulobacter henricii isolate PhyCEm-80 99.1 Lower Kaimai, BOP May-14 18 Caulobacter henricii isolate PhyCEm-80 99.2 Lower Kaimai, BOP May-14 19 Caulobacter henricii isolate PhyCEm-80 99.0 Lower Kaimai, BOP May-14 20 Caulobacter henricii isolate PhyCEm-80 99.3 Mt Albert, Auckland May-14 21 Caulobacter henricii isolate PhyCEm-80 99.2 Pt Chevalier, Auckland May-14 22 Caulobacter henricii isolate PhyCEm-80 99.2 Bethells Beach Jan-14 23 Caulobacter henricii isolate PhyCEm-80 99.3 Bethells Beach Jan-14 24 Caulobacter henricii isolate PhyCEm-80 99.0 Avondale, Auckland May-14 25 Caulobacter henricii isolate PhyCEm-80 99.0 Raumati, Kapiti May-14
All C. henricii isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Considering the global distribution of C. henricii across diverse habitats, extensive host range including plants with a long history in NZ, documented ability to disperse widely
December 2013 EPA0327 26
Application Form To obtain a determination of whether an organism is a new organism with dust events, and the ability to readily isolate the bacterium in New Zealand, it is highly likely that A. ferrireducens is ubiquitous wherever suitable environmental conditions exist within New Zealand and is not a new organism.
December 2013 EPA0327 27
Application Form To obtain a determination of whether an organism is a new organism
14. Polaromonas ginsengisoli
[See summary table for evidence supporting ubiquity]
At least 18 isolates of Polaromonas ginsengisoli, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 15 samples (mostly soil) collected from multiple sites spanning the North Island between August 2012 and May 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these isolates had between 98.8% to 100% (mean 99.8%) identity to known strains over an average alignment of 1003 bases (range 260 to 1069 bases).
Blast Sample Isolate Blast hit identity (%) Sample site date 1 Polaromonas ginsengisoli strain: Gsoil 115 100 Raumati, Kapiti Aug-12 2 Polaromonas ginsengisoli strain: Gsoil 115 100 Raumati, Kapiti Aug-12 3 Polaromonas ginsengisoli strain: Gsoil 115 100 Raumati, Kapiti Aug-12 4 Polaromonas ginsengisoli strain: Gsoil 115 100 Raumati, Kapiti Aug-12 5 Polaromonas ginsengisoli strain: Gsoil 115 100 Pokaka, National Park Aug-12 6 Polaromonas ginsengisoli strain: Gsoil 115 98.8 Hukatere, Northland Aug-12 7 Polaromonas ginsengisoli strain: Gsoil 115 99.9 Hukatere, Northland Aug-12 8 Polaromonas ginsengisoli strain: Gsoil 115 99.8 Hukatere, Northland Aug-12 9 Polaromonas ginsengisoli strain: Gsoil 115 100 Hukatere, Northland Aug-12 10 Polaromonas ginsengisoli strain: Gsoil 115 99.6 Bethells Beach Jan-14 11 Polaromonas ginsengisoli strain: Gsoil 115 99.8 Bethells Beach Jan-14 12 Polaromonas ginsengisoli strain: Gsoil 115 100 Bethells Beach Jan-14 13 Polaromonas ginsengisoli strain: Gsoil 115 99.9 Bethells Beach Jan-14 14 Polaromonas ginsengisoli strain: Gsoil 115 99.8 Raumati, Kapiti Jan-14 15 Polaromonas ginsengisoli strain: Gsoil 115 99.5 Lower Kaimai, BOP May-14 16 Polaromonas ginsengisoli strain: Gsoil 115 99.5 Lower Kaimai, BOP May-14 17 Polaromonas ginsengisoli strain: Gsoil 115 99.7 Hukatere, Northland Aug-12 18 Polaromonas ginsengisoli strain: Gsoil 115 99.6 Raumati, Kapiti May-14
All P. ginsengisoli isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Considering the global distribution of P. ginsengisoli across different habitats, and the ability to readily isolate the bacterium in New Zealand, it is highly likely that P. ginsengisoli is ubiquitous wherever suitable environmental conditions exist within New Zealand and is not a new organism.
December 2013 EPA0327 28
Application Form To obtain a determination of whether an organism is a new organism
15. Mucilaginibacter dorajii
[See summary table for evidence supporting ubiquity]
At least 4 isolates of Mucilaginibacter dorajii, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 4 samples (mostly soil) collected from locations in Auckland and Kapiti between August 2012 and January 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these isolates had between 97% to 99.6% (mean 98.2%) identity to known strains over an average alignment of 938 bases (range 794 to 1089 bases).
Blast identity Isolate Blast hit (%) Sample site Sample date 1 Mucilaginibacter dorajii strain DR-f4 99.6 Raumati, Kapiti Aug-12 2 Mucilaginibacter dorajii strain DR-f4 98.0 Otaki, Kapiti Jan-14 3 Mucilaginibacter dorajii strain DR-f4 97.0 Otaki, Kapiti Jan-14 4 Mucilaginibacter dorajii strain DR-f4 98.1 Bethells Beach Jan-14
All M. dorajii isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Considering the ubiquity of a documented host (Platycodon grandiflorum) of M. dorajii, both globally and in NZ, and the amenability of M. dorajii to be isolated from soils within NZ, it is highly likely that M. dorajii is not a new organism and is present wherever suitable environmental conditions exist.
December 2013 EPA0327 29
Application Form To obtain a determination of whether an organism is a new organism
16. Duganella zoogloeiodes (synonym Zooglea ramigera)
[See summary table for evidence supporting ubiquity]
At least 17 isolates of Duganella zoogloeiodes, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 11 samples (mostly soil) collected from locations between Northland and Kapiti between August 2012 and January 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these isolates had between 98.1% to 99.8% (mean 99.3%) identity to known strains over an average alignment of 954 bases (range 327 to 1113 bases).
Blast identity Sample Isolate Blast hit (%) Sample site date 1 Duganella zoogloeoides strain A1RO1 99.5 Raumati, Kapiti Aug-12 2 Duganella zoogloeoides strain A1RO1 99.4 Raumati, Kapiti Aug-12 3 Duganella zoogloeoides strain A1RO1 99.7 Hukatere, Northland Aug-12 4 Duganella zoogloeoides strain: L B-H 98.9 Hukatere, Northland Aug-12 5 Duganella zoogloeoides strain A1RO1 99.2 Hukatere , Northland Aug-12 6 Duganella zoogloeoides strain NBRC 102465 99.1 Hukatere, Northland Aug-12 7 Duganella zoogloeoides strain A1RO1 98.1 Hukatere, Northland Aug-12 8 Duganella zoogloeoides strain A1RO1 99.8 Hukatere, Northland Aug-12 9 Duganella zoogloeoides strain A1RO1 99.8 Hukatere, Northland Aug-12 10 Duganella zoogloeoides strain A1RO1 98.5 Hukatere, Northland Aug-12 11 Duganella zoogloeoides strain A1RO1 99.6 Pukekohe, Auckland Apr-13 12 Duganella zoogloeoides strain A1RO1 99.6 Pukekohe, Auckland Apr-13 13 Duganella zoogloeoides strain A1RO1 98.9 Pokaka, nr National Park Apr-13 14 Duganella zoogloeoides strain A1RO1 99.3 Otaki, Kapiti Dec-13 15 Duganella zoogloeoides strain A1RO1 99.5 Otaki, Kapiti Jan-14 16 Duganella zoogloeoides strain A1RO1 98.9 Otaki, Kapiti Jan-14 17 Duganella zoogloeoides strain A1RO1 99.5 Raumati, Kapiti Jan-14
All D. zoogloeiodes isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Considering the global distribution of D. zoogloeiodes across diverse environments, and the ability to readily isolate the bacterium in New Zealand, it is highly likely that D. zoogloeiodes is ubiquitous wherever suitable environmental conditions exist within New Zealand and is not a new organism.
December 2013 EPA0327 30
Application Form To obtain a determination of whether an organism is a new organism
17. Herbaspirillum huttiense (synonym Pseudomonas huttiense)
Presence in NZ before July 29 1998
Eight cultures of H. huttiense (synonym Pseudomonas huttiensis) were isolated from distilled water samples from New Zealand in 196219. Leifson (1962) commented that the 8 cultures represented about 20% of the total number of isolates from New Zealand samples, and the organism “must have been one of the major types of bacteria in the water”. Additional NZ isolates
At least 15 isolates of Herbaspirillum huttiense, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 12 samples (mostly soil) collected from locations between Northland and Kapiti between August 2012 and January 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these isolates had between 99.1% to 100% (mean 99.9%) identity to known strains over an average alignment of 923 bases (range 534 to 1101 bases).
Blast identity Sample Isolate Blast hit (%) Sample site date 1 Herbaspirillum huttiense strain NBRC 102521 100 Hukatere, Northland Aug-12 2 Herbaspirillum huttiense strain NBRC 102521 99.8 Hukatere, Northland Aug-12 3 Herbaspirillum huttiense strain NBRC 102521 99.9 Hukatere, Northland Aug-12 4 Herbaspirillum huttiense strain NBRC 102521 100 Pukekohe, Auckland Apr-13 5 Herbaspirillum huttiense strain NBRC 102521 100 Bethells Beach Apr-13 6 Herbaspirillum huttiense strain NBRC 102521 99.5 Bethells Beach Apr-13 7 Herbaspirillum huttiense strain NBRC 102521 100 Raumati, Kapiti Apr-13 8 Herbaspirillum huttiense strain NBRC 102521 100 Raumati, Kapiti Apr-13 9 Herbaspirillum huttiense strain NBRC 102521 100 Raumati, Kapiti Apr-13 10 Herbaspirillum huttiense strain LAMA 1109 100 Raumati, Kapiti Apr-13 11 Herbaspirillum huttiense strain NBRC 102521 100 Pokaka, National Park Apr-13 12 Herbaspirillum huttiense strain NBRC 102521 99.9 Otaki, Kapiti Jan-14 13 Herbaspirillum huttiense strain CCBAU 61429 100 Bethells Beach May-14 14 Herbaspirillum huttiense strain NBRC 102521 100 Bethells Beach Jan-14 15 Herbaspirillum huttiense strain AU6965 99.1 Raumati, Kapiti Jan-14
December 2013 EPA0327 31
Application Form To obtain a determination of whether an organism is a new organism
18. Stenotrophomonas rhizophila 19. Stenotrophomonas chelatiphaga
[See summary table for evidence supporting ubiquity]
At least 22 isolates of Stenotrophomonas rhizophila, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 20 samples (mostly soil) collected from locations throughout the North Island between August 2008 and October 2014. BLAST alignment 18 of the 16S rRNA gene sequences showed these isolates had between 97.7% to 100% (mean 99.1%) identity to known strains over an average alignment of 788 bases (range 674 to 980 bases).
Blast Sample Isolate Blast hit identity (%) Sample site date 1 Stenotrophomonas rhizophila strain e-p10 99 Paparoa, Kaipara Aug-08 2 Stenotrophomonas rhizophila strain e-p10 99.3 Paparoa, Kaipara Aug-08 3 Stenotrophomonas rhizophila strain e-p10 98.4 Paparoa, Kaipara Aug-08 4 Stenotrophomonas rhizophila strain e-p10 99 Paparoa, Kaipara Aug-08 5 Stenotrophomonas rhizophila strain e-p10 98.9 Paparoa, Kaipara Aug-08 6 Stenotrophomonas rhizophila strain e-p10 97.2 Paparoa, Kaipara Aug-08 7 Stenotrophomonas rhizophila strain e-p10 98.4 Paparoa, Kaipara Aug-08 8 Stenotrophomonas rhizophila strain e-p10 99.6 Paparoa, Kaipara Aug-08 9 Stenotrophomonas rhizophila strain e-p10 99.6 Paparoa, Kaipara Aug-08 10 Stenotrophomonas rhizophila strain e-p10 99.1 Paparoa, Kaipara Aug-08 11 Stenotrophomonas rhizophila strain e-p10 99.7 Pokaka, National Park Sep-08 12 Stenotrophomonas rhizophila strain e-p10 100 Pokaka, National Park Sep-08 13 Stenotrophomonas rhizophila strain e-p10 99.9 Napier, Hawkes Bay Sep-08 14 Stenotrophomonas rhizophila strain GL5 97.7 Northland, Wellington Sep-08 15 Stenotrophomonas rhizophila strain e-p10 99.8 Northland, Wellington Sep-08 16 Stenotrophomonas rhizophila strain KUDC1826 98.6 Northland, Wellington Sep-08 17 Stenotrophomonas rhizophila strain e-p10 98.8 Northland, Wellington Sep-08 18 Stenotrophomonas rhizophila strain e-p10 98.9 Titirangi, Auckland Sep-08 19 Stenotrophomonas rhizophila strain e-p10 98 Manakau, Kapiti Aug-11 20 Stenotrophomonas rhizophila strain e-p10 100 Otaki, Kapiti Dec-13 21 Stenotrophomonas rhizophila strain KUDC1826 99.9 Raumati, Kapiti Jan-14 22 Stenotrophomonas rhizophila strain 14A 99.9 Waikiwi, Waikato Oct-14
December 2013 EPA0327 32
Application Form To obtain a determination of whether an organism is a new organism
At least 6 isolates of Stenotrophomonas chelatiphaga, as classified by 16S rRNA sequences by RDP Sequence Match 17, have been isolated in New Zealand from 5 samples (mostly soil) collected from locations between Northland and Kapiti between August 2008 and April 2013. BLAST alignment 18 of the 16S rRNA gene sequences showed these isolates had between 98.9% to 100% (mean 99.5%) identity to known strains over an average alignment of 879 bases (range 727 to 1060 bases). Blast identity Sample Isolate Blast hit (%) Sample site date 1 Stenotrophomonas chelatiphaga strain STY47 99.5 Paparoa, Kaipara Aug-08 2 Stenotrophomonas chelatiphaga strain LPM-5 98.9 Te Puke, BOP Mar-11 3 Stenotrophomonas chelatiphaga strain LPM-5 99 Hukatere, Northland Aug-11 4 Stenotrophomonas chelatiphaga strain ATY59 100 Raumati, Kapiti Aug-12 5 Stenotrophomonas chelatiphaga strain KLBMP 4821 99.8 Raumati, Kapiti Aug-12 6 Stenotrophomonas chelatiphaga strain KLBMP 4821 99.7 Bethells Beach Apr-13
All S. rhizophila and S. chelatiphaga isolates are naturally occurring and not genetically modified organisms, they do not appear on the unwanted organisms register or notifiable organisms list and have not been previously eradicated from New Zealand.
Considering the global distribution of S. rhizophila and S. chelatiphaga across diverse environments, their wide host range, and the ability to readily isolate the bacteria in New Zealand, it is highly likely that S. rhizophila and S. chelatiphaga are ubiquitous wherever suitable environmental conditions exist within New Zealand and are not new organisms.
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Application Form To obtain a determination of whether an organism is a new organism Summary table: Evidence of global ubiquity and New Zealand isolates Name Synonym Number Dates and Sample Number Other International International sample matrix; date of of NZ locations of matrix; of areas records in isolates records isolates isolation number where NZ location of samples samples collected collected at different time points Bacillus 10 Apr-13, Jan- Soil; 9 6 NOC001224 – Multiple Original isolates from soil, mud and water from psychrosaccharolytic 14, May-14; samples approval to locations in lowland fields, streams and lakes 1,20. Also us (ex Larkin and National Park, hold organism USA and Asia isolated from sediments, soil and water/ice Stokes 1967) Priest et Kapiti, Bay of in (India and samples from cold deserts, lakes and mountains al. 1989, sp. nov., Plenty, containment China). 21,22, as well as rhizosphere of yellow-tuft nom. rev Auckland for reaching (Alyssum murale) 23,24. Yellow-tuft is known to be and research present in NZ with no import restrictions*.; 1966, 2003, 2013, 2014, 2015. Bosea lupini De 10 May-14, Aug- Soil; 9 7 None Belgium, Root nodule of lupin (Lupinus polyphyllus)2 and Meyer and Willems 12; Auckland, samples China soil (Genbank KF730777). The plant host, L. 2012, sp. nov. Kapiti, polyphyllus is native to North America, but was Northland, introduced to eastern Europe in the late National Park, 1700s/early 1800s, and has naturalised in New Bay of Plenty Zealand since 1958 25,26; 2012, 2013 Duganella Pseudoduganell 25 Sep-08, Aug- Soil; 21 10 None Asia (China, A wide range of different soils 27,3,28,29, material violaceinigra Li et al. a violaceinigra 12, Apr-13, samples Japan, South associated with potato 30 and Vetiver grass roots 2004, sp. nov. (Li et al. 2004) Jan-14, May- Korea), 31, desert sand forming intercontinental dust that Kämpfer et al. 14, Jun-14; Europe can travel large distances 32. Both potato 2012, comb. Northland, (Slovakia and (Solanum tuberosum) and vetiver grass (Vetiveria nov. Napier, Italy), North zizanioides) are widely cultivated worldwide, Duganella Auckland, America (USA with a long history in NZ33. Unpublished violaceusniger National Park, and Canada) sequence from isolate from natural environment Kapiti, and Africa (Genbank HM032838); 2004, 2007, 2008, 2010, Waikato, Bay (Chad) 2011, 2012
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Application Form To obtain a determination of whether an organism is a new organism of Plenty Flavobacterium 5 Aug-11, Jan- Soil; 5 3 None China, Lake Frozen glacier soil 4, numerically significant glaciei Zhang et al. 14, May-14; samples Baikal in member associated with lake biofilm 34 and 2006, sp. nov. Kapiti, Bay of Siberia, Russia Baikalospongia sp. sponges 35, reactor seeded Plenty with wastewater 36; 2006, 2010, 2014 Flavobacterium Cytophaga 7 Aug-12, Mar- Soil; 7 4 NOC99013 - United Freshwater sediment 37, rice field 38,39, sea ice 40 saccharophilum saccharophila 13, Apr-13, samples approval to Kingdom, and soil 41.; 1976, 1980, 1992-1995, 1999 (Bernardet et al. (ex Agbo and May-14; hold organism Japan, 1996) Moss 1979) Northland, in Antarctica, Reichenbach Auckland, containment Greece 1989 National Park, for reaching Bay of Plenty and research Massilia niastensis 43 Aug-11, Aug- Soil; 36 11 None South Korea, Air samples 5, soil 42–44, endophyte associated Weon et al. 2009, sp. 12, Apr-13, samples India, China, with orange foxtail (Alopecurus aequalis SoboI) nov. Dec-13, May- Inner 45. Orange foxtail is a common species of grass 14; Northland, Mongolia, that can be found in both islands of NZ, and its Auckland, Spain, presence in NZ has been documented from at National Park, Netherlands, least the 1930s 46. Unpublished sequences Napier, Bay of Canada matching M. niastensis from soil crust in Inner Plenty, Kapiti Mongolia (Genbank JF496496, JF496359, JF496281) and air in India (Genbank HM209779); 2009, 2010, 2011, 2012, 2013, 2014. Pseudomonas 7 Aug-11, Aug- Soil; 6 4 NZ isolates: Canada, Effluent or residue from a wide range of different abietaniphila Mohn 12, Jan-14; samples isolated from Japan, industrial processes 6,47,48, soil 49, associated with et al. 1999, sp. nov. Northland, pasture Vietnam, grapevine (Vitis vinifera subsp. vinifera cultivar Napier, Kapiti rhizosphere Lebanon, 'Sauvignon Blanc') 50. Sauvignon Blanc vines were sampled from Austria introduced to NZ in 1973 and are now planted all Whatawhata, over the country51.; 1994, 1989, 2014, 2006-2007 Waikato in 2010 and Wellington in 2008 Pseudomonas 20 Aug-12, Feb- Soil; 12 5 NZ isolate: Austria, Water under dog cage 7, surface of copper coins psychrotolerans 13, Apr-13, samples found in soil Europe, 52,53, air 54,55, associated with a wide range of Hauser et al. 2004, Jan-14; and Mexico, plants 56–60, surface water 61, cave pools 62, table
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Application Form To obtain a determination of whether an organism is a new organism sp. nov. Northland, associated Indonesia, olive wastewaters 63, catfish fillets 64, glacier 65; Auckland, plant material Thailand, USA, 2004, 2006, 2007, 2009-2010, 2010, 2011, 2014 National Park, sampled at Greece, Japan, Kapiti Napier in Majorca June, 2009 Rhizobacter fulvus Methylibium 13 Aug-12, Apr- Soil; 9 5 None South Korea, A wide range of soils from temperate and cold (Yoon et al. 2007) fulvum Yoon et 13, Jan-14; samples Japan, China, environments 66–70, glacial ice cores 71, endophyte Stackebrandt et al. al. 2007. Northland, India, USA, of drunken horse grass (Achnatherum inebrians) 2009, comb. nov. Auckland, Czech 72, Bishop pines (Pinus muricata) 73 and aquatic National Park, Republic, reed (Phragmites australis) (Tanaka et al., 2012), Kapiti South Africa, mesocosms of wetland 75 and aquaculture 76, Chilean groundwater 77,78, wastewater 79, cold Patagonia, oligotrophic lake 80, BioGranulated Activated Antarctica Carbon (BioGAC) reactors 81, and intestinal mucosa of grass carp (Ctenopharyngodon idellus). Grass carp was first introduced to NZ in 196682, and Bishop pines has been planted in NZ since 197283; 2007, 2011, 2005, 2008, 2009, 2013, 2012, 2010-2011, 2007, 2006 Sphingobium 6 Aug-12, Apr- Soil; 4 3 None India, China Soil 8 activated sluge 84; 2010, 2013 quisquiliarum Bala et 13, May-13, samples al. 2010, sp. nov. Jan-14; Northland, Kapiti Sphingobium Sphingomonas 21 Aug-08, Mar- Soil; 14 9 Determined to Japan, USA, Various brands of bottled water originating in the yanoikuyae 85 yanoikuyae 9 10, Aug-11, samples not be present Spain, USA 86, a polluted stream in the USA87, mine Aug-12, Feb- in NZ in non- Portugal, tailings in the USA88, the rhizoplane of spinach 13, Apr-13, statutory China seedlings in Japan89 , endophytic bacteria Dec-13, May- advice in 2010 isolated from Asian ginseng in China90 (listed in 14; Northland, (PNZ1000024) the MPI Plant Biosecurity Index as available to Kaipara, import without restrictions), porous limestone in Auckland, Spain91, drinking water in Portugal92; 1973, 2001, Katikati, 2004, 2010, 2011, 2013. National Park, Kapiti
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Application Form To obtain a determination of whether an organism is a new organism Albidiferax Rhodoferax 11 Aug-12, Jan- Soil; 8 5 None Finland, USA, Rainbow trout eggs 93, freshwater sediments 10,94, ferrireducens corrig. ferrireducens 14, May-14; samples China, groundwater 95–97, wastewater sludge 98, stool (Finneran et al. 2003) Finneran et al. Northland, Pakistan, samples 99, steel pilings in marine environment Ramana and Sasikala 2003. Auckland, Antarctica, 100, feathers of Eastern Bluebirds (Sialia sialis) 101, 2009, comb. nov. Kapiti Japan, UK, glacier sediment 102, sediment cores 103, Arctic, hydrothermal vents 104, drinking water 105; 2014, Hungary, 2003, 2012, 2005, 2006-2007, 2011, 2005-2008, South Korea 2007, 2009, 2006 Caulobacter henricii 25 Aug-12, Apr- Soil; 21 12 NZ isolate: UK, South Originally observed and isolated from various Poindexter 1964 13, Jan-14, samples isolated in Korea, pond water collected at different dates between May-14; 2010 from Antarctica, 1953 and 1962 11, air samples during Asian dust Northland, pasture Russia, USA, event when large amount of dust from the desert Auckland, rhizosphere Italy, in China is transported to Korea and Japan 106, National Park, sampled from Germany, water column of cold Lake Hoare, McMurdo Dry Bay of Plenty, Winchmore, China, Hong Valleys, Antarctica 107, water samples from Kapiti Canterbury Kong, Finland various lakes across Russia 108, including the bacterial community associated with bacterioplankton 109, Lake Baikal 110, rhizosphere of maize (Zea mays) 111, alpine soil 112–114, soils 115– 119, various brands of bottled water originating from the USA 86, surfaces of sponge Mycale adhaerens 120; April 2007-March 2008, 2010, 1981-1985, 1999, 1990, 2007, 2008, 2009, 2003- 2004, 2013 Polaromonas 18 Aug-12, Jan- Soil; 15 6 None South Korea, Soil from ginseng field (Genbank AB245355 ginsengisoli 14, May-14; samples China, unpublished), glacier 121, freshwater lake 122. Northland, Germany Ginseng is listed in the MPI Plant Biosecurity Auckland, Index as available to import without restrictions; National Park, 2005, 2007, 2004 Bay of Plenty, Kapiti Mucilaginibacter 4 Aug-12, Jan- Soil; 4 3 None South Korea Rhizosphere of bell flower (Platycodon dorajii Kim et al. 14; Auckland, samples grandiflorum) 12.The plant host, bell flower, is 2011, sp. nov. Kapiti available for sale in NZ, and is not a new organism (http://www.egmontseeds.co.nz/-c- 1/platycodon-astra-mix-f1-hybrid); 2010
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Application Form To obtain a determination of whether an organism is a new organism Duganella Zoogloea 17 Aug-12, Apr- Soil; 11 5 Isolated from Japan, India, Originally described in 1867124, Duganella zoogloeides13 ramigera 123 13, Dec-13, samples pasture Lithuania, zoogloeoides has long been considered the Jan-14; rhizosphere in Portugal, typical activated sludge bacterium responsible Northland, Cantebury China, West for the formation of activated sludge flocs 125. It Auckland, 2010, held in a Africa, is readily isolated from wastewater environments National Park, private NZ Germany, 13, the type strain being isolated from trickling Kapiti collection Spain filter wastewater treatment system. For example, (according to hybridisation with DNA probes found D. Landcare zoogloeoides in activated sludge samples taken database). from Spain and Germany 125. 16S rRNA sequence Detected in analysis revealed the presence of D. zoogloeoides two Auckland in ice core retrieved from the Malan Ice Cap on wastewater the Tibetan Plateau 126. Also identified in samples treatment of hydroponic moss in Japan127, water from plants (Brown Himalayan lakes21, soil in Senegal128, bottled 2001, mineral water in Portugal (JQ689172), intestinal unpublished microbiota of salmon in a Lithuanian urban river master’s (JF915351), the surface of carbonate rocks in thesis, China (JN650583); 1995, 2000, 2004, 2011, 2012, University of 2013. Auckland) Herbaspirillum Pseudomonas 15 Aug-12, Apr- Soil; 12 6 Eight cultures China, Paddy fields in China (FJ266337), associated with huttiense (Leifson huttiensis 13, Jan-14, samples were isolated Singapore, Lablab purpureus (Hyacinth bean, present in NZ) 1962) Ding and Leifson 1962 May-14; from NZ Brazil in China129, endophytes of Jatropha cultivars (MPI Yokota 2004, comb. Northland, distilled water Plant Biosecurity Index lists all Jatropha cultivars nov. Auckland, samples in as available to import without restrictions) in National Park, 196219; NZ Singapore (JQ659769), from sugarcane in Brasil Kapiti isolate: NZRM (JX155400); 2009, 2010, 2011, 2012. 4502 - found in sputum. Also found in soil and associated plant material sampled at Napier on
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Application Form To obtain a determination of whether an organism is a new organism June, 2009 Stenotrophomonas 22 Aug-08, Sep- Soil; 20 9 Isolates from Germany, Strains have been identified from the rhizophila 15 08, Aug-11, samples soil and Svalbard, rhizospheres of oilseed rape and potatoes in Dec-13, Jan- associated India, Iran, Germany130; the geocaulosphere of 14, Oct-14; plant material China, potato130; glacial sediments from Svalbard Kaipara, in Napier, Thailand, 131; oil contaminated soil in China Auckland, Northland and
Waikato, Wellington on Italy, USA (GQ359325) and Iran (GU586312); the National Park, June 2008 rhizosphere of peas in India (GU186108); 132 Hawkes Bay, endophytes from Asian ginseng in China Kapiti, (listed in the MPI Plant Biosecurity Index as Wellington available to import without restrictions); mangrove sediment in Thailand (AB819386); olive oil from southern Italy (KJ534282); a spacecraft assembly clean room in the USA 133. 1993, 1996, 2009, 2013, 2014 Stenotrophomonas 6 Aug-08, Mar- Soil; 5 5 None USA, Strains have been identified from sewage chelatiphaga 134 11, Aug-12, samples South Korea, sludge in Russia 134; citrus roots in Florida 135; Apr-13; Russia and tobacco (JF460768) and wheat (FJ493060) in Northland, China China; oyster mushrooms in South Korea Kaipara, (JX971549). 2008, 2009, 2011, 2012 Auckland, Bay of Plenty, Kapiti *Biosecurity status of plant hosts were checked on the Ministry for Primary Industries PBI database http://www1.maf.govt.nz/cgi- bin/bioindex/bioindex.pl
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Application Form To obtain a determination of whether an organism is a new organism
References
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68. Pradhan, S. et al. Bacterial biodiversity from Roopkund Glacier, Himalayan mountain ranges, India. Extremophiles 14, 377–95 (2010).
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69. Chengalroyen, M. D. & Dabbs, E. R. Identification of a gene responsible for amido black decolorization isolated from Amycolatopsis orientalis. World J. Microbiol. Biotechnol. 29, 625– 33 (2013).
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74. Tanaka, Y. et al. Microbial Community Analysis in the Roots of Aquatic Plants and Isolation of Novel Microbes Including an Organism of the Candidate Phylum OP10. Microbes Environ. 27, 149–157 (2012).
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81. Aslett, D., Haas, J. & Hyman, M. Identification of tertiary butyl alcohol (TBA)-utilizing organisms in BioGAC reactors using 13C-DNA stable isotope probing. Biodegradation 22, 961– 72 (2011).
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94. Kojima, H., Fukuhara, H. & Fukui, M. Community structure of microorganisms associated with reddish-brown iron-rich snow. Syst. Appl. Microbiol. 32, 429–37 (2009).
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95. Zaa, C. L. Y., McLean, J. E., Dupont, R. R., Norton, J. M. & Sorensen, D. L. Dechlorinating and Iron Reducing Bacteria Distribution in a TCE-Contaminated Aquifer. Ground Water Monit. Remediat. 30, 46–57 (2010).
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97. Táncsics, A. et al. Investigation of catechol 2,3-dioxygenase and 16S rRNA gene diversity in hypoxic, petroleum hydrocarbon contaminated groundwater. Syst. Appl. Microbiol. 33, 398– 406 (2010).
98. Yao, S., Ni, J., Chen, Q. & Borthwick, A. G. L. Enrichment and characterization of a bacteria consortium capable of heterotrophic nitrification and aerobic denitrification at low temperature. Bioresour. Technol. 127, 151–7 (2013).
99. Kapoor, A. et al. A newly identified bocavirus species in human stool. J. Infect. Dis. 199, 196– 200 (2009).
100. Hicks, R. E. Structure of Bacterial Communities associated with accelerated corrosive loss of port transportation infrastructure. (2007). at
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103. WILKINS, M. J., LIVENS, F. R., VAUGHAN, D. J., BEADLE, I. & LLOYD, J. R. The influence of microbial redox cycling on radionuclide mobility in the subsurface at a low-level radioactive waste storage site. Geobiology 5, 293–301 (2007).
104. Ovreas, L., Johannessen, T., Jorgensen, S., Thorseth, I. H. & Pedersen, R. B. Diversity of Microorganisms Associated With low Temperature Iron Deposits at the 71°N Hydrothermal Vent Field Along the Arctic Mid-Ocean Ridge. AGU Fall Meet. Abstr. -1, 0992 (2007).
105. Li, D. et al. Characterization of bacterial community structure in a drinking water distribution system during an occurrence of red water. Appl. Environ. Microbiol. 76, 7171–80 (2010).
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107. Clocksin, K. M., Jung, D. O. & Madigan, M. T. Cold-active chemoorganotrophic bacteria from permanently ice-covered Lake Hoare, McMurdo Dry Valleys, Antarctica. Appl. Environ. Microbiol. 73, 3077–83 (2007).
108. Ariskina, E. V., Chernousova, E. Y., Lapteva, N. A. & Akimov, V. N. Evaluation of the taxonomic diversity of prosthecate bacteria belonging to the genera Brevundimonas and Caulobacter
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isolated from various eurasian ecosystems by analysis of the 16S rRNA genes. Microbiology 80, 403–410 (2011).
109. Trusova, M. Y. & Gladyshev, M. I. Species Composition of Winter Bacterioplankton in Two Siberian Ponds Determined by the 16S rRNA Sequence Analysis. Dokl. Biol. Sci. 382, 51–54 (2002).
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111. Schmalenberger, A. & Tebbe, C. C. Bacterial community composition in the rhizosphere of a transgenic, herbicide-resistant maize (Zea mays) and comparison to its non-transgenic cultivar Bosphore. FEMS Microbiol. Ecol. 40, 29–37 (2002).
112. Lipson, D. A. & Schmidt, S. K. Seasonal Changes in an Alpine Soil Bacterial Community in the Colorado Rocky Mountains. Appl. Environ. Microbiol. 70, 2867–2879 (2004).
113. Zhang, D. & Margesin, R. Characterization of culturable heterotrophic bacteria in hydrocarbon-contaminated soil from an alpine former military site. World J. Microbiol. Biotechnol. 30, 1717–24 (2014).
114. Liu, G.-X. et al. Variations in soil culturable bacteria communities and biochemical characteristics in the Dongkemadi glacier forefield along a chronosequence. Folia Microbiol. (Praha). 57, 485–94 (2012).
115. Macedo, A. J., Timmis, K. N. & Abraham, W.-R. Widespread capacity to metabolize polychlorinated biphenyls by diverse microbial communities in soils with no significant exposure to PCB contamination. Environ. Microbiol. 9, 1890–7 (2007).
116. DeAngelis, K. M., Lindow, S. E. & Firestone, M. K. Bacterial quorum sensing and nitrogen cycling in rhizosphere soil. FEMS Microbiol. Ecol. 66, 197–207 (2008).
117. Liu, Y.-J., Liu, S.-J., Drake, H. L. & Horn, M. A. Alphaproteobacteria dominate active 2-methyl- 4-chlorophenoxyacetic acid herbicide degraders in agricultural soil and drilosphere. Environ. Microbiol. 13, 991–1009 (2011).
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121. Zhang, W. et al. Diversity of Bacterial Communities in the Snowcover at Tianshan Number 1 Glacier and its Relation to Climate and Environment. Geomicrobiol. J. 29, 459–469 (2012).
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122. Kim, O.-S., Junier, P., Imhoff, J. F. & Witzel, K.-P. Comparative analysis of ammonia-oxidizing bacterial communities in two lakes in North Germany and the Baltic Sea. Arch. für Hydrobiol. 167, 335–350 (2006).
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135. Trivedi, P., Spann, T. & Wang, N. Isolation and characterization of beneficial bacteria associated with citrus roots in Florida. Microb. Ecol. 62, 324–36 (2011).
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20. Checklist This checklist is to be completed by the applicant
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