Biolphilately Vol-64 No-3
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(Equatorial Spitting Cobra) Venom a P
The Journal of Venomous Animals and Toxins including Tropical Diseases ISSN 1678-9199 | 2011 | volume 17 | issue 4 | pages 451-459 Biochemical and toxinological characterization of Naja sumatrana ER P (Equatorial spitting cobra) venom A P Yap MKK (1), Tan NH (1), Fung SY (1) RIGINAL O (1) Department of Molecular Medicine, Center for Natural Products and Drug Research (CENAR), Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. Abstract: The lethal and enzymatic activities of venom from Naja sumatrana (Equatorial spitting cobra) were determined and compared to venoms from three other Southeast Asian cobras (Naja sputatrix, Naja siamensis and Naja kaouthia). All four venoms exhibited the common characteristic enzymatic activities of Asiatic cobra venoms: low protease, phosphodiesterase, alkaline phosphomonoesterase and L-amino acid oxidase activities, moderately high acetylcholinesterase and hyaluronidase activities and high phospholipase A2. Fractionation of N. sumatrana venom by Resource® S cation exchange chromatography (GE Healthcare, USA) yielded nine major protein peaks, with all except the acidic protein peak being lethal to mice. Most of the protein peaks exhibit enzymatic activities, and L-amino acid oxidase, alkaline phosphomonoesterase, acetylcholinesterase, 5’-nucleotidase and hyaluronidase exist in multiple forms. Comparison of the Resource® S chromatograms of the four cobra venoms clearly indicates that the protein composition of N. sumatrana venom is distinct from venoms of the other two spitting cobras, N. sputatrix (Javan spitting cobra) and N. siamensis (Indochinese spitting cobra). The results support the revised systematics of the Asiatic cobra based on multivariate analysis of morphological characters. The three spitting cobra venoms exhibit two common features: the presence of basic, potentially pharmacologically active phospholipases A2 and a high content of polypeptide cardiotoxin, suggesting that the pathophysiological actions of the three spitting cobra venoms may be similar. -
WHO Guidance on Management of Snakebites
GUIDELINES FOR THE MANAGEMENT OF SNAKEBITES 2nd Edition GUIDELINES FOR THE MANAGEMENT OF SNAKEBITES 2nd Edition 1. 2. 3. 4. ISBN 978-92-9022- © World Health Organization 2016 2nd Edition All rights reserved. Requests for publications, or for permission to reproduce or translate WHO publications, whether for sale or for noncommercial distribution, can be obtained from Publishing and Sales, World Health Organization, Regional Office for South-East Asia, Indraprastha Estate, Mahatma Gandhi Marg, New Delhi-110 002, India (fax: +91-11-23370197; e-mail: publications@ searo.who.int). The designations employed and the presentation of the material in this publication do not imply the expression of any opinion whatsoever on the part of the World Health Organization concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. Dotted lines on maps represent approximate border lines for which there may not yet be full agreement. The mention of specific companies or of certain manufacturers’ products does not imply that they are endorsed or recommended by the World Health Organization in preference to others of a similar nature that are not mentioned. Errors and omissions excepted, the names of proprietary products are distinguished by initial capital letters. All reasonable precautions have been taken by the World Health Organization to verify the information contained in this publication. However, the published material is being distributed without warranty of any kind, either expressed or implied. The responsibility for the interpretation and use of the material lies with the reader. In no event shall the World Health Organization be liable for damages arising from its use. -
Cobra Risk Assessment
Invasive animal risk assessment Biosecurity Queensland Agriculture Fisheries and Department of Cobra (all species) Steve Csurhes and Paul Fisher First published 2010 Updated 2016 Pest animal risk assessment © State of Queensland, 2016. The Queensland Government supports and encourages the dissemination and exchange of its information. The copyright in this publication is licensed under a Creative Commons Attribution 3.0 Australia (CC BY) licence. You must keep intact the copyright notice and attribute the State of Queensland as the source of the publication. Note: Some content in this publication may have different licence terms as indicated. For more information on this licence visit http://creativecommons.org/licenses/ by/3.0/au/deed.en" http://creativecommons.org/licenses/by/3.0/au/deed.en Photo: Image from Wikimedia Commons (this image is reproduced under the terms of a GNU Free Documentation License) Invasive animal risk assessment: Cobra 2 Contents Summary 4 Introduction 5 Identity and taxonomy 5 Taxonomy 3 Description 5 Diet 5 Reproduction 6 Predators and diseases 6 Origin and distribution 7 Status in Australia and Queensland 8 Preferred habitat 9 History as a pest elsewhere 9 Uses 9 Pest potential in Queensland 10 Climate match 10 Habitat suitability 10 Broad natural geographic range 11 Generalist diet 11 Venom production 11 Disease 11 Numerical risk analysis 11 References 12 Attachment 1 13 Invasive animal risk assessment: Cobra 3 Summary The common name ‘cobra’ applies to 30 species in 7 genera within the family Elapidae, all of which can produce a hood when threatened. All cobra species are venomous. As a group, cobras have an extensive distribution over large parts of Africa, Asia, Malaysia and Indonesia. -
The Medical Threat of Mamba Envenoming in Sub-Saharan Africa
Downloaded from orbit.dtu.dk on: Oct 06, 2021 The medical threat of mamba envenoming in sub-Saharan Africa revealed by genus- wide analysis of venom composition, toxicity and antivenomics profiling of available antivenoms Ainsworth, Stuart; Petras, Daniel; Engmark, Mikael; Süssmuth, Roderich D.; Whiteley, Gareth; Albulescu, Laura-Oana; Kazandjian, Taline D.; Wagstaff, Simon C.; Rowley, Paul; Wüster, Wolfgang Total number of authors: 16 Published in: Journal of Proteomics Link to article, DOI: 10.1016/j.jprot.2017.08.016 Publication date: 2018 Document Version Peer reviewed version Link back to DTU Orbit Citation (APA): Ainsworth, S., Petras, D., Engmark, M., Süssmuth, R. D., Whiteley, G., Albulescu, L-O., Kazandjian, T. D., Wagstaff, S. C., Rowley, P., Wüster, W., Dorrestein, P. C., Arias, A. S., M. Gutierrez, J., Harrison, R., Casewell, N. R., & Calvete, J. J. (2018). The medical threat of mamba envenoming in sub-Saharan Africa revealed by genus-wide analysis of venom composition, toxicity and antivenomics profiling of available antivenoms. Journal of Proteomics, 172, 173-189. https://doi.org/10.1016/j.jprot.2017.08.016 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. -
Creatures That Can Kill You 5
AROUND THE WORLD PREVIEWITH WSNAKES Preview Snakes slither through nearly every country on Earth. Most snakes are harmless, but some of them are are anything but. In this chapter, we will travel from India to Australia to Africa. The scariest, the deadliest, and the most feared snakes—they’re all here! 1 Around The World With Snakes AROUNDIn a rice THEfield in WORLD India, a young WITH worker namedSNAKES Abhay stepped carefully around the plants he tended. As always, Abhay listened for any quick rustling through the leaves or, worse yet, In a ricea sudden field hissing. in India, But as thea youngsky began workergrowing named Abhaydark stepped with rain carefully clouds, Abhay around became the careless plants he in his hurry to finish his job. He moved quickly tended.through As always, some tallerAbhay plants listened that blocked for any his quick rustlingview through of the ground. the leaves or, worse yet, a sudden hissing. But as the sky began growing dark with rain clouds, Abhay became careless in his hurry to finish his job. He moved quickly through some taller plants that blocked his view of the ground. Then, just at the edge of the field, Abhay felt something very strange. It was as if a thick cord of rope had been yanked beneath his left foot. 3 4 MARIE NOBLE In that same instant, Abhay saw a flash of brown and gold rise up before him—the dreaded Indian cobra. It was not the first time he had seen this terrifying snake. From the time he was a young child, Abhay had often seen these 6- to 10-foot snakes slithering through the village where he lived. -
Proteomic and Genomic Characterisation of Venom Proteins from Oxyuranus Species
This file is part of the following reference: Welton, Ronelle Ellen (2005) Proteomic and genomic characterisation of venom proteins from Oxyuranus species. PhD thesis, James Cook University Access to this file is available from: http://eprints.jcu.edu.au/11938 Chapter 1 Introduction and literature review Chapter 1 Introduction and literature review 1.1 INTRODUCTION Animal venoms are an evolutionary adaptation to immobilise and digest prey and are used secondarily as a defence mechanism (Tu and Dekker, 1991). Intriguingly, evolutionary adaptations have produced a variety of venom proteins with specific actions and targets. A cocktail of protein and peptide toxins have varying molecular compositions, and these unique components have evolved for differing species to quickly and specifically target their prey. The compositions of venoms differ, with components varying within the toxins of spiders, stinging fish, jellyfish, octopi, cone shells, ticks, ants and snakes. Toxins have evolved for the varying mode of actions within different organisms, yet many enzymes are common to different venoms including L-amino oxidases, esterases, aminopeptidases, hyaluronidases, triphosphatases, alkaline phosphomonoesterases, 2 2 phospholipases, phosphodiesterases, serine-metalloproteases and Ca +IMg +-activated proteases. The enzymes found in venoms fall into one or more pharmacological groups including those which possess neurotoxic (causing paralysis or interfering with nervous system function), myotoxic (damaging muscle), haemotoxic (affecting the blood, -
A Pan-Specific Antiserum Produced by a Novel Immunization Strategy
www.nature.com/scientificreports OPEN A pan-specifc antiserum produced by a novel immunization strategy shows a high spectrum of neutralization against neurotoxic snake venoms Kavi Ratanabanangkoon1,2 ✉ , Kae Yi Tan3, Kritsada Pruksaphon4, Chaiya Klinpayom5, José María Gutiérrez6, Naeem H. Quraishi7 & Choo Hock Tan8 ✉ Snakebite envenomation is a neglected tropical disease of high mortality and morbidity largely due to insufcient supply of efective and afordable antivenoms. Snake antivenoms are mostly efective against the venoms used in their production. It is thus crucial that efective and afordable antivenom(s) with wide para-specifcity, capable of neutralizing the venoms of a large number of snakes, be produced. Here we studied the pan-specifc antiserum prepared previously by a novel immunization strategy involving the exposure of horses to a ‘diverse toxin repertoire’ consisting of 12 neurotoxic Asian snake toxin fractions/ venoms from six species. This antiserum was previously shown to exhibit wide para-specifcity by neutralizing 11 homologous and 16 heterologous venoms from Asia and Africa. We now show that the antiserum can neutralize 9 out of 10 additional neurotoxic venoms. Altogether, 36 snake venoms belonging to 10 genera from 4 continents were neutralized by the antiserum. Toxin profles previously generated using proteomic techniques of these 36 venoms identifed α-neurotoxins, β-neurotoxins, and cytotoxins as predominant toxins presumably neutralized by the antiserum. The bases for the wide para-specifcity of the antiserum are discussed. These fndings indicate that it is feasible to generate antivenoms of wide para-specifcity against elapid neurotoxic venoms from diferent regions in the world and raises the possibility of a universal neurotoxic antivenom. -
Lizards & Snakes: Alive!
LIZARDSLIZARDS && SNAKES:SNAKES: ALIVE!ALIVE! EDUCATOR’SEDUCATOR’S GUIDEGUIDE www.sdnhm.org/exhibits/lizardsandsnakeswww.sdnhm.org/exhibits/lizardsandsnakes Inside: • Suggestions to Help You Come Prepared • Must-Read Key Concepts and Background Information • Strategies for Teaching in the Exhibition • Activities to Extend Learning Back in the Classroom • Map of the Exhibition to Guide Your Visit • Correlations to California State Standards Special thanks to the Ellen Browning Scripps Foundation and the Nordson Corporation Foundation for providing underwriting support of the Teacher’s Guide KEYKEY CONCEPTSCONCEPTS Squamates—legged and legless lizards, including snakes—are among the most successful vertebrates on Earth. Found everywhere but the coldest and highest places on the planet, 8,000 species make squamates more diverse than mammals. Remarkable adaptations in behavior, shape, movement, and feeding contribute to the success of this huge and ancient group. BEHAVIOR Over 45O species of snakes (yet only two species of lizards) An animal’s ability to sense and respond to its environment is are considered to be dangerously venomous. Snake venom is a crucial for survival. Some squamates, like iguanas, rely heavily poisonous “soup” of enzymes with harmful effects—including on vision to locate food, and use their pliable tongues to grab nervous system failure and tissue damage—that subdue prey. it. Other squamates, like snakes, evolved effective chemore- The venom also begins to break down the prey from the inside ception and use their smooth hard tongues to transfer before the snake starts to eat it. Venom is delivered through a molecular clues from the environment to sensory organs in wide array of teeth. -
Very Venomous, But...- Snakes of the Wet Tropics
No.80 January 2004 Notes from Very venomous but ... the Australia is home to some of the most venomous snakes in the world. Why? Editor It is possible that strong venom may little chance to fight back. There are six main snake families have evolved chiefly as a self-defence in Australia – elapids (venomous strategy. It is interesting to look at the While coastal and inland taipans eat snakes, the largest group), habits of different venomous snakes. only mammals, other venomous colubrids (‘harmless’ snakes) Some, such as the coastal taipan snakes feed largely on reptiles and pythons, blindsnakes, filesnakes (Oxyuranus scutellatus), bite their frogs. Venom acts slowly on these and seasnakes. prey quickly, delivering a large amount ‘cold-blooded’ creatures with slow of venom, and then let go. The strong metabolic rates, so perhaps it needs to Australia is the only continent venom means that the prey doesn’t be especially strong. In addition, as where venomous snakes (70 get far before succumbing so the many prey species develop a degree of percent) outnumber non- snake is able to follow at a safe immunity to snake venom, a form of venomous ones. Despite this, as distance. Taipans eat only mammals – evolutionary arms race may have been the graph on page one illustrates, which are able to bite back, viciously. taking place. very few deaths result from snake This strategy therefore allows the bites. It is estimated that between snake to avoid injury. … not necessarily deadly 50 000 and 60 000 people die of On the other hand, the most Some Australian snakes may be snake bite each year around the particularly venomous, but they are world. -
Research Journal of Pharmaceutical, Biological and Chemical
ISSN: 0975-8585 Research Journal of Pharmaceutical, Biological and Chemical Sciences Diversity of Squamates (Scaled Reptiles) in Selected Urban Areas of Cagayan de Oro City, Misamis Oriental. John C Naelga*, Daniel Robert P Tayag, Hazel L Yañez, and Astrid L Sinco. Xavier University – Ateneo De Cagayan, Kinaadman Resource Center. ABSTRACT This study was conducted to provide baseline information on the local urban diversity of squamates in the selected areas of Barangay Kauswagan, Barangay Balulang, and Barangay FS Catanico in Cagayan de Oro City. These urban sites are close to the river and are likely to be inhabited by reptiles. Each site had at least ten (10) points and was sampled no less than five (5) times in the months of September to November 2016 using homemade traps and the Cruising-Transect walk method. One representative per species was preserved. A total of two hundred sixty-seven (267) individuals, grouped into four (4) families and ten (10) species were found in the sampling areas. Six (6) snake species were identified, namely: Boiga cynodon, Naja samarensis, Chrysopelea paradisi, Gonyosoma oxycephalum, Coelegnathus erythrurus eryhtrurus, and Dendrelaphis pictus; while four (4) species were lizards namely: Gekko gecko, Hemidactylus platyurus, Lamprolepis smaragdina philippinica, and Eutropis multifasciata.In Barangay Kauswagan, Hemidactylus platyurus was the most abundant (RA= 52.94%). In Barangay Balulang, the most abundant species was Hemidactylus platyurus (RA= 43.82%). In Barangay FS Catanico, the most abundant was Hemidactylus platyurus (RA= 40.16%). The area with the highest species diversity was Barangay FS Catanico (H= 1.36), followed by Barangay Balulang (H= 1.28), and Barangay Kauswagan (H= 1.08). -
North American Save the Rhinos Campaign
NORTH AMERICAN SAVE THE RHINOS CAMPAIGN AZA RAG/SSP CONTENTS Foreword Evan Blumer, John Lukas, and Tom Foose 1 Introduction to the Info Pack Julie Anton Dunn 5 Section I: Campaign Guidelines 9 Participation and Registration 10 Registration Form 12 Use of Images 13 List of Images and Additional Information on the CD-ROM 14 Use of Logos and Font 15 Fundraising Money Transfer Details 17 Sharing Information and Materials 19 Certificates and Awards 20 Rhino Campaigners 22 Thanks and Acknowledgements 23 Section II: Beneficiary Projects 25 Selecting the Beneficiary Projects 26 Expressing a Preference 27 The Common Need to Protect Rhinos 28 Sumatran Rhino Program 29 Indian Rhino Program 32 Black Rhino Program 35 EAZA Campaign Projects 39 EAZA’s Waiting-list Projects 40 Section III: Awareness, Education and Fundraising 41 Awareness: 42 • 10 good reasons to get involved in the North American Save the Rhinos Campaign 42 • We do not have rhinos; how can we get involved? 44 Education: 45 • Suggested education activities 45 • Rhino Rangers 48 • Rhino Cards Zimbabwe 49 • Catch the Poacher 51 Fundraising: 53 • Suggested fundraising activities 53 • How will my money be used? 55 Section IV: Rhino Information 57 The Evolution of the Rhinoceros 58 The Discovery of African Rhinos 62 First Sightings of Asian Rhinos 63 The Five Species of Rhino and their Subspecies: 64 • Rhino Population Numbers and Distribution 64 • White Rhinoceros 66 • Black Rhinoceros 70 • Greater One-Horned or Indian Rhinoceros 75 • Sumatran Rhinoceros 79 • Javan Rhinoceros 84 The Web -
Comparative Studies of the Venom of a New Taipan Species, Oxyuranus Temporalis, with Other Members of Its Genus
Toxins 2014, 6, 1979-1995; doi:10.3390/toxins6071979 OPEN ACCESS toxins ISSN 2072-6651 www.mdpi.com/journal/toxins Article Comparative Studies of the Venom of a New Taipan Species, Oxyuranus temporalis, with Other Members of Its Genus Carmel M. Barber 1, Frank Madaras 2, Richard K. Turnbull 3, Terry Morley 4, Nathan Dunstan 5, Luke Allen 5, Tim Kuchel 3, Peter Mirtschin 2 and Wayne C. Hodgson 1,* 1 Monash Venom Group, Department of Pharmacology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Victoria 3168, Australia; E-Mail: [email protected] 2 Venom Science Pty Ltd, Tanunda, South Australia 5352, Australia; E-Mails: [email protected] (F.M.); [email protected] (P.M.) 3 SA Pathology, IMVS Veterinary Services, Gilles Plains, South Australia 5086, Australia; E-Mails: [email protected] (R.K.T.); [email protected] (T.K.) 4 Adelaide Zoo, Adelaide, South Australia 5000, Australia; E-Mail: [email protected] 5 Venom Supplies, Tanunda, South Australia, South Australia 5352, Australia; E-Mails: [email protected] (N.D.); [email protected] (L.A.) * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +61-3-9905-4861; Fax: +61-3-9905-2547. Received: 5 May 2014; in revised form: 11 June 2014 / Accepted: 16 June 2014 / Published: 2 July 2014 Abstract: Taipans are highly venomous Australo-Papuan elapids. A new species of taipan, the Western Desert Taipan (Oxyuranus temporalis), has been discovered with two specimens housed in captivity at the Adelaide Zoo. This study is the first investigation of O.