Isolation of Dye from Sporophore of Xylaria Polymorpha for Textile Dyeing and Spectrophotometric Characterization of Dyed Fabrics
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JAM 3(4) 2017 pp 162 - 173 162 ISSN Online: 2349-7785 Isolation of Dye from Sporophore of Xylaria polymorpha for Textile Dyeing and Spectrophotometric Characterization of Dyed Fabrics Rakesh Kumar 1*, Y.C. Tripathi 1, Ambika 2, Jyoti Sharma 2, Binita Kumari 2 and Amit Pandey 2 1 Chemistry Division, Forest Research Institute, P.O. New Forest, Dehradun - 248006, India 2 Forest Pathology Division, Forest Research Institute, P.O. New Forest, Dehradun - 248006, India Received 21 September 2017; accepted in revised form 26 October 2017 Abstract: The present study was aimed at isolating natural dye from Xylaria polymorpha, a wood rot fungus with club shaped fruiting body by standardizing the extraction protocol. Different fabrics viz. silk, wool and cotton were dyed using the extracted dye employing different common mordants of metallic salts and few natural mordants and the resultant shades, fastness properties and colour coordinates were compared on the test fabrics. The dye produced different shades of blackish brown on the dyed fabrics. Based on the highest absorbance, best performance of the dye was recorded with wool followed by silk whereas least absorbance was observed with cotton. The dye showed high affinity with wool and silk fabrics but relatively low affinity with cotton. It is found to hold very good to excellent colourfastness (rating 4-5) on wool and silk fabrics but good to very good (rating 3-4) on cotton against various colour retarding factors. The study evidently indicated that X. polymorpha dye is suitable for dyeing wool, silk and cotton thus establishing the fungus as a potential source of textile dye of biological origin. This is the first report on X. polymorpha as a viable source of textile dye of biological origin. Key words: Xylaria polymorpha, natural dye, textile dyeing, colourfastness, colour coordinates. Introduction to children even before birth 2. Industrial organic The textile industries consume huge volumes waste, especially aromatic textile dyes, are con- of pure water which is converted into wastewa- sidered one of the most abundant and hazardous ter contaminated with wide spectrum of hazard- pollutants particularly, azo dyes are reported to ous chemicals which possess high toxicity value be mutagenic and are linked to increasing inci- (EC50:3-6 %), if remain untreated 1. Organically dences of cancer amongst textile workers 3-7. bound chlorine, a known carcinogen has been re- Even drinking water after treatment has been re- ported in about 40 percent of globally used ported to carry the effluents of dyeing industries colourants and hence significant amount of envi- discharged into the river indicating that the adopted ronmental degradation and human illnesses is effluent treatment based on pre-chlorination, floc- caused by textile effluent. These chemicals evapo- culation, coagulation and flotation, commonly used rate into the air and enter into human body either by DWTP (Demineralization Water Treatment through breathing or epidermal absorption thereby Plants), are not completely efficient to remove demonstrating allergic reactions and causing harm these dyes. These facts have been supported with *Corresponding author (Rakesh Kumar) E-mail: < [email protected] > © 2017, Har Krishan Bhalla & Sons Rakesh Kumar et al. / JAM 3(4) 2017 pp 162 - 173 163 mutagenic activity detected in such wastewater mophores of mushroom dyes contain a variety of 8. Hence looking to the toxicity of synthetic dyes fascinating organic compounds. Their pigmenta- coupled with worldwide consciousness over bet- tion may vary with the age and some undergo ter environment, the revival of interest in natural distinctive colour changes on bruising; therefore, dyes are increasing. Natural dyes are less toxic, the colours of mushrooms are one of the essen- less polluting, less health hazardous, non-carcino- tial features used in their identification. Further- genic and non-poisonous. Added to this, they are more, the pigments of mushrooms may protect harmonizing colours, gentle, soft and subtle, and the organism from UV damage and bacterial at- create a restful effect. Above all, they are envi- tack or play a role as insect attractants 9. ronment-friendly and can be recycled after use. Pigments production from microbes is advan- Natural dyes find application in many ways in tageous over other sources because microorgan- clothes, foods, pharmaceuticals, paintings, paints isms can grow rapidly which may lead to a high and cosmetics. However in spite of its toxicity efficiency in quality and quantity of the product and environmental problems associated with the Monascus species are widely used as a micro- synthetic dyes, the textile manufacturers are not bial source for natural red pigment production 10. yet finding proper enticement in switching over to Monascus red pigments are of polyketide origin natural dyes for being more expensive than their and are used commercially as non-toxic colorants synthetic counterparts. It is due the lack of scien- for colouring rice wine, “Koji”, soybean, cheese tifically validated information of dye-yielding and red meat 11. The red pigments have attracted sources, sustained supply of raw material, incon- worldwide commercial interest 12, 13, but little in- sistent yield and colour quality that warrants sin- formation is available on the production of the pig- cere scientific interventions if production and use ment by other microbial sources. It has been re- of natural dyes is to be made a commercial real- ported that Penicillium may be the potential can- ity. Despite lots of available traditional wisdom in didate to produce polyketide structure compounds this context, a very limited number of such infor- 12. Natural dyeing using the fungi derived colorants mation has been properly documented and scien- has been reported in some countries. However, tifically validated. The technology of utilizing natu- published reports of scientific research on the sub- ral dyes in the modern clothing industry is also ject are scanty 14. Although the natural dyeing has relatively new and is still being improved upon. age-old traditions, however, with systematic R&D The problem of consistent supply in sufficient interventions, efficiency and colourfastness of dye quantity at appropriate price can be ensured by can be improved and a range of hues can be gen- increasing R&D work on natural dyes and tech- erated. Procedural improvement for dye extrac- nological improvements and finding new resources tion, dyeing and mordanting through systemically of natural dye. In recent year there has been in- designed research ultimately lead to better qual- creasing interest in using microorganism as a ity dye production and textile dyeing. The present source of natural colours owing to cost efficiency, work was thus aimed at extracting natural dyes sustained raw material supply, less labour and land from Xylaria polymorpha and its characteriza- required and non-hazardous production process. tion to ascertain its suitability as commercially ac- However, very little efforts have so far been made ceptable textile dye. to use fungi as source of natural dyes despite the fact that certain species can yield considerable Materials and methods amount of good quality colouring substance for Fungus and fabrics textile applications. Use of fungi for production The fruiting body of Xylaria polymorpha of natural dye has certain advantages over plant (1.245 g) was collected from the premises of For- dye that includes availability of material in desired est Research Institute (FRI) Dehradun, India dur- quantity, intense and consistent colours and can ing the months of August-September, 2016. The fulfill need of consumers and industries for over- collected fungal material was authenticated by the all development of natural dye sector. The chro- Plant Pathologist of Forest Pathology Division, Rakesh Kumar et al. / JAM 3(4) 2017 pp 162 - 173 164 Forest Research Institute, Dehradun. Pure silk, was carried out by keeping the MLR as standard- wool and cotton textile fabrics were purchased ized above and extracted for varying time rang- from Gandhi Ashram, an authorized outlet of ing from 15 to 90 minutes. The dye extracts ob- KVIC at Dehradun, India. The fabrics were tained from each of the experiments were cooled, washed with non-ionic detergent (1 % owf) for filtered through whatman-1, and made to 100 mL., 30 minutes, rinsed and dried at room tempera- then 1 mL. of aliquot from each of the extracts ture. The fabric material was wetted in water for were diluted to 100 mL and subjected to optical 30 min prior to dyeing or mordanting density (OD) measurement. Based on the high- est value of OD, optimum values of MLR, alkali Chemical & analytical content and duration for dye extraction were de- All the chemicals viz., acetic acid, alum, cop- termined. Natural dye from fruiting body of X. per sulphate, ferrous sulphate, potassium dichro- polymorpha was extracted using calculated val- mate and stannous chloride used as mordants were ues of MLR, duration and alkali content. of L.R. grade of chemicals and procured from local dealers. Pure Khadi silk, wool and cotton Dyeing of silk, wool and cotton fabrics were used in dyeing experiments. The U.V. spec- Dyeing on silk, wool and cotton samples was tra were recorded on a UV spectrophotometer carried out by post mordanting method, which pro- (Chemito make; Model 2700. The CIEL*a*b*, duced improved shades in terms of hue and dark- hue, chroma and K/S values were determined on ness. The dye bath was set 10 g per liter of the a Reference Bench-top Spectrophotometer dye by keeping the material to liquor ratio of 1:30. (Make-Gretag Mac Beth; Colour-Eye 7000A). The dye bath was heated up to a temperature of Colour fastness of the different types of dyed fab- 80-850C and maintaining the solution level for 45 ric against washing, light, perspiration and rub- minutes for dyeing the silk and wool samples and bing were measured by using Laundrometer a temperature of 90-950C was maintained for cot- (Make: Metrex Scientific Instrument (P) Ltd.), ton for 60 minutes.