Reporter Assays for Ebola Virus Nucleoprotein Oligomerization
bioRxiv preprint doi: https://doi.org/10.1101/454314; this version posted December 10, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Article 2 Reporter assays for Ebola virus nucleoprotein 3 oligomerization, virion-like particle budding, and 4 minigenome activity reveal the importance of 5 nucleoprotein amino acid position 111 6 Aaron E. Lin 1,2,3,12,*, William E. Diehl 4,12, Yingyun Cai 5, Courtney L. Finch 5, Chidiebere Akusobi 7 6, Robert N. Kirchdoerfer 7, Laura Bollinger 5, Stephen F. Schaffner 2,3, Elizabeth A. Brown 2,3, 8 Erica Ollmann Saphire 8, Kristian G. Andersen 9,10, Jens H. Kuhn 5,13, Jeremy Luban 4,13, Pardis C. 9 Sabeti 1,2,3,11,13,* 10 1 Harvard Program in Virology, Harvard Medical School, Boston, MA 02115, USA 11 2 FAS Center for Systems Biology, Department of Organismic and Evolutionary Biology, Harvard 12 University, Cambridge, MA 02138, USA 13 3 Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA 14 4 Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA 15 5 Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National 16 Institutes of Health, Frederick, MD 21702, USA 17 6 Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Boston, 18 MA 02120, USA 19 7 Department of Biochemistry,
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