Frdc Final Report

Total Page:16

File Type:pdf, Size:1020Kb

Frdc Final Report FRDC FINAL REPORT DEVELOPMENT OF COMMERCIAL PRODUCTION SYSTEMS FOR MUD CRAB (Scylla serrata) AQUACULTURE IN AUSTRALIA: HATCHERY & NURSERY Colin Shelley, Graham Williams, Ian Ruscoe, Brian Paterson, David Mann July 2008 FRDC Project No. 2000/210 ISBN 978-0-7245-4730-2 Copyright Fisheries Research and Development Corporation, Northern Territory Department of Primary Industry, Fisheries and Mines and the Queensland Department of Primary Industry and Fisheries, 2008. This work is copyright. Except as permitted under the Copyright Act 1968 (Cth), no part of this publication may be reproduced by any process, electronic or otherwise, without the specific written permission of the copyright owners. Neither may information be stored electronically in any form whatsoever without such permission. The Fisheries Research and Development Corporation plans, invests in and manages fisheries research and development throughout Australia. It is a statutory authority within the portfolio of the federal Minister for Agriculture, Fisheries and Forestry, jointly funded by the Australian Government and the fishing industry. Title Page Principal Investigator: Colin C. Shelley1 Darwin Aquaculture Centre, Department of Primary Industry, Fisheries and Mines, GPO Box 3000, Darwin, Northern Territory 0801, Australia Correspondence: 1Aquaculture Industry Development and Policy, Fisheries, Department of Primary Industries and Fisheries, GPO Box 46, Brisbane, Queensland 4001, Australia Phone: 07 3224 2257 Fax: 07 3239 0439 Email: [email protected] Co-Investigators: Graham R. Williams, Darwin Aquaculture Centre, Department of Primary Industry, Fisheries and Mines, GPO Box 3000, Darwin, Northern Territory 0801, Australia Ian Ruscoe, Darwin Aquaculture Centre, Department of Primary Industry, Fisheries and Mines, GPO Box 3000, Darwin, Northern Territory 0801, Australia Brian Paterson, Bribie Island Aquaculture Research Centre, 144 North Street, Woorim, Queensland 4507, Australia David Mann, Bribie Island Aquaculture Research Centre, 144 North Street, Woorim, Queensland 4507, Australia MUD CRAB AQUACULTURE Page i Table of Contents Principal Investigator: ........................................................................................................................................i Correspondence: ...............................................................................................................................................i Co-Investigators: .................................................................................................................................................i List of Tables .....................................................................................................................................................ix List of Figures ..................................................................................................................................................xii Principal Investigator: .......................................................................................................................................1 Objectives ...........................................................................................................................................................1 Non Technical Summary ..................................................................................................................................1 Outcomes Achieved ...........................................................................................................................................1 Executive summary ...........................................................................................................................................2 Keywords ...........................................................................................................................................................................3 Acknowledgements............................................................................................................................................................4 Background........................................................................................................................................................................5 Need ..................................................................................................................................................................................5 Objectives ..........................................................................................................................................................................6 Historical and geographical effort .............................................................................................................................. 8 Feeds and feeding regimes ....................................................................................................................................... 8 Bacterial control ......................................................................................................................................................... 9 System design ........................................................................................................................................................... 9 Larviculture development in this project................................................................................................................... 10 Standard Operating Procedures ....................................................................................................................10 Standard Operating Procedures for Larviculture at DAC................................................................................................. 11 Broodstock. ...............................................................................................................................................................11 Standard design for multi-factorial, small-scale trials................................................................................................11 Statistical analysis ...........................................................................................................................................................12 Standard design for large-scale, replicated trials (1000 L). .........................................................................13 McRoberts Larval Tanks ..................................................................................................................................................14 Feeds ....................................................................................................................................................................... 15 Algae ........................................................................................................................................................................ 15 Rotifers..................................................................................................................................................................... 15 Artemia..................................................................................................................................................................... 15 Salinity control.......................................................................................................................................................... 16 Culture water pre-treatment ..................................................................................................................................... 16 Routine operation of 1000 L culture tanks ............................................................................................................... 16 Effect of culture water pre-treatments and tank colour on survival of mud crab (Scylla serrata) larvae. (DAC–Batch 1) ......................................................................................................................................17 Introduction ......................................................................................................................................................................17 Methods ...........................................................................................................................................................................17 Small-scale trial ...............................................................................................................................................................17 Large-scale trial ...............................................................................................................................................................17 Results .............................................................................................................................................................................19 Large-scale trial ...............................................................................................................................................................19 Discussion .......................................................................................................................................................................21 Investigation of the effect of culture water pre-treatments on survival of mud crab (Scylla serrata) larvae. (DAC–Batch 2) ......................................................................................................................................24
Recommended publications
  • Effect of Feed on the Growth and Survival of Long Eyed Swimming
    Soundarapandian et al., 2:3 http://dx.doi.org/10.4172/scientificreports681 Open Access Open Access Scientific Reports Scientific Reports Research Article OpenOpen Access Access Effect of Feed on the Growth and Survival of Long Eyed Swimming Crab Podophthalmus vigil Fabricius (Crustacea: Decapoda) Soundarapandian P1*, Ravichandran S2 and Varadharajan D1 1Faculty of Marine Sciences, Centre of Advanced Study in Marine Biology, Annamalai University, Tamil Nadu, India 2Department of Zoology, Government Arts College, Kumbakonam, Tamil Nadu, India Abstract Food is considered to be the most potent factor affecting growth. Attempts to develop diets for culture of crabs have resulted in a variety of feeds. The absence of suitable feed either pellet or live food which can promote growth and survival is considered to be the most important lacuna in cultivation of crabs. So searching of economically viable feed to optimize the growth and survival in crabs are very much essential. In the present study the weight gain was higher in the crabs offered with Acetes sp. (86 g) followed by clam meat fed animals (47 g). The crabs fed with minimum amount of Acetes sp. (152 g) and maximum of clam meat (182 g). The FCR value was better in Acetes sp. (1.8) fed animal rather than clam meat fed animals (3.8). The survival rate was higher in Acetes sp. fed animals (92%) and lowest survival (72%) was observed in animals fed with clam meat. The survival was reasonable for both the feeds. But higher survival rate was reported in the animals fed with Acetes sp. than that of clam meat.
    [Show full text]
  • Diversity and Abundance of Intertidal Crabs at the East Swamp- Managed Areas in Segara-Anakan Cilacap, Central Java, Indonesia
    Deutscher Tropentag, October 8-10, 2003, Göttingen “Technological and Institutional Innovations for Sustainable Rural Development” Diversity and Abundance of Intertidal Crabs at the East Swamp- Managed Areas in Segara-Anakan Cilacap, Central Java, Indonesia 1 2 2 MOH. HUSEIN SASTRANEGARA , HELEEN FERMON AND MICHAEL MÜHLENBERG 1 University of Jenderal Soedirman, Faculty of Biology, 53122 Purwokerto, Indonesia, e-mail: [email protected] 2 Georg August Universität Göttingen, Centre for Nature Conservation, 37075 Göttingen, Germany, e-mail: [email protected] 2 Georg August Universität Göttingen, Centre for Nature Conservation, 37075 Göttingen, Germany, e-mail: [email protected] Abstract Mangrove forests possess a high diversity and abundance of crabs in Central Java. The conservation of mangrove forests into prawn ponds causes depletion of supply of river sediments and loss of property. The main objective of this study is to compare the diversity and abundance of intertidal crabs in undisturbed, crab hunting, logging and prawn pond areas that were different in percent mangrove canopy covers and percent sediment textures. In each area, two transect lines were installed to analyse the percent mangrove canopy cover, and also the percent sediment texture comparing to trilinier plot. In total, 16,353 intertidal crab individuals in 13 species were sampled. Differences in observed number and estimated number of species (ACE, Chao1), as well as number of individuals, diversity indices and evenness between the four studied mangrove areas were all highly significant. Monthly fluctuation of intertidal crab diversity was more constant in the undisturbed area with a high mangrove coverage (90%) compared to the crab hunting, the logging and the prawn pond area with a coverage of 89%, 33% and 0%, respectively.
    [Show full text]
  • Rearing Enhancement of Ovalipes Trimaculatus (Crustacea: Portunidae
    www.nature.com/scientificreports OPEN Rearing enhancement of Ovalipes trimaculatus (Crustacea: Portunidae) zoea I by feeding on Artemia persimilis nauplii enriched with alternative microalgal diets Antonela Martelli1,3*, Elena S. Barbieri1,3, Jimena B. Dima2 & Pedro J. Barón1 The southern surf crab Ovalipes trimaculatus (de Haan, 1833) presents a high potential for aquaculture. In this study, we analyze the benefts of diferent dietary treatments on its molt success and ftness of larval stages. Artemia persimilis nauplii were enriched with monospecifc (Nannochloropsis oculata, Tetraselmis suecica, Dunaliella salina, Isochrysis galbana and Chaetoceros gracilis) and multispecifc (Mix) microalgal diets twice a day over a 48-h period. Mean total length (TL), growth instar number (I) and gut fullness rate (GFR) of nauplii showed signifcant diferences between dietary treatments at several sampling times, optimal results being observed in those providing Mix. Artemia nauplii grown under most experimental dietary treatments reached the capture size limit for Ovalipes trimaculatus zoea I (700 µm) within 24 h. After that time interval, Mix-enriched nauplii were amongst those with higher protein contents. Ovalipes trimaculatus zoea I fed on Artemia nauplii enriched during 24 h under diferent dietary treatments showed signifcant diferences in survival, inter-molt duration, molting success to zoea II and motility. Optimal results were observed in zoea I fed on Mix-enriched Artemia nauplii. This work not only represents a frst step towards the dietary optimization for O. trimaculatus zoeae rearing but also provides the frst results on the use of enriched A. persimilis. Portunid crabs stand out as highly valued resources for fsheries and aquaculture because of their export potential and high nutritional value1.
    [Show full text]
  • A New Pathogenic Virus in the Caribbean Spiny Lobster Panulirus Argus from the Florida Keys
    DISEASES OF AQUATIC ORGANISMS Vol. 59: 109–118, 2004 Published May 5 Dis Aquat Org A new pathogenic virus in the Caribbean spiny lobster Panulirus argus from the Florida Keys Jeffrey D. Shields1,*, Donald C. Behringer Jr2 1Virginia Institute of Marine Science, The College of William & Mary, Gloucester Point, Virginia 23062, USA 2Department of Biological Sciences, Old Dominion University, Norfolk, Virginia 23529, USA ABSTRACT: A pathogenic virus was diagnosed from juvenile Caribbean spiny lobsters Panulirus argus from the Florida Keys. Moribund lobsters had characteristically milky hemolymph that did not clot. Altered hyalinocytes and semigranulocytes, but not granulocytes, were observed with light microscopy. Infected hemocytes had emarginated, condensed chromatin, hypertrophied nuclei and faint eosinophilic Cowdry-type-A inclusions. In some cases, infected cells were observed in soft con- nective tissues. With electron microscopy, unenveloped, nonoccluded, icosahedral virions (182 ± 9 nm SD) were diffusely spread around the inner periphery of the nuclear envelope. Virions also occurred in loose aggregates in the cytoplasm or were free in the hemolymph. Assembly of the nucleocapsid occurred entirely within the nucleus of the infected cells. Within the virogenic stroma, blunt rod-like structures or whorls of electron-dense granular material were apparently associated with viral assembly. The prevalence of overt infections, defined as lethargic animals with milky hemolymph, ranged from 6 to 8% with certain foci reaching prevalences of 37%. The disease was transmissible to uninfected lobsters using inoculations of raw hemolymph from infected animals. Inoculated animals became moribund 5 to 7 d before dying and they began dying after 30 to 80 d post-exposure.
    [Show full text]
  • The Effect of Different Ph and Photoperiod Regimens on The
    Short communication: The effect of different pH and photoperiod regimens on the survival rate and developmental period of the larvae of Portunus pelagicus (Decapoda, Brachyura, Portunidae) Item Type article Authors Ravi, R.; Manisseri, M.K. Download date 28/09/2021 09:20:50 Link to Item http://hdl.handle.net/1834/37376 Iranian Journal of Fisheries Sciences Short Communication 12(2) 490-499 2013 __________________________________________________________________________________________ The effect of different pH and photoperiod regimens on the survival rate and developmental period of the larvae of Portunus pelagicus (Decapoda, Brachyura, Portunidae) Ravi R.*; Manisseri M. K. Received: December 2011 Accepted: August 2012 -Marine Biodiversity Division, Central Marine Fisheries Research Institute, Post Box No. 1603, Kochi-682 018, India *Corresponding author’s e mail: [email protected] Keywords: Portunus pelagicus, Photoperiod, Larval rearing, Larval development, Larval survival. Crustacean larval development occurs (Waddy and Aiken, 1991), cannibalism within a narrow range of environmental (Hecht and Pienaar, 1993) and swimming parameters (Sastry, 1983). Among the activity and hence metabolism (Gardner, abiotic characteristics that influence the 1996). developmental period and survival rate of The effects of photoperiod on the larvae, pH and photoperiod are of great larval survival have been studied in several importance. High and low levels of pH are species such as the common shrimp known to adversely affect the physiology Crangon
    [Show full text]
  • Mangrove Wetlands
    WETLAND MANAGEMENT PROFILE MANGROVE WETLANDS Mangrove wetlands are characterised by Description trees that are uniquely adapted to tolerate Mangroves are woody plants, usually trees (woodland daily or intermittent inundation by the sea. to forest) but also shrubs, growing in the intertidal Distinctive animals are nurtured in their zone and able to withstand periods of inundation by sheltered waters and mazes of exposed seawater each day. In this profile, the term ”mangrove mangrove roots; others live in the underlying wetland” is used to refer to wetlands that are characterised by dominance of mangroves. mud. A familiar sight on the coast throughout “Mangrove associates” are non-woody plants that Queensland, mangrove wetlands provide mainly or commonly occur in mangrove wetlands. frontline defence against storm damage, The terms “mangrove forest” and “mangrove swamp” enhance reef water quality and sustain are sometimes used in regard to particular types of fishing and tourism industries. These habitat mangrove wetland or to mangrove wetland in general. assets can be preserved and loss and degradation (through pollution and changed MANGROVE wetlands are characterised water flows) averted by raising awareness of by the dominance of mangroves — woody the benefits of mangrove wetlands and by implementing integrated plans for land and plants growing in the intertidal zone and resource use in the coastal zone. able to withstand periods of inundation by seawater each day. Situated in land zone 1, mangrove wetlands are invariably in the intertidal zone and sometimes extend narrowly for many kilometres inland along tidal rivers, also around some near-shore islands. Accordingly, they are below the level of Highest Astronomical Tide (HAT) and typically are in the lower, most frequently and deeply inundated parts of the intertidal zone.
    [Show full text]
  • Evaluating the Environmental Drivers of Mud Crab (Scylla Serrata) Catches in Australia
    Evaluating the environmental drivers of mud crab (Scylla serrata) catches in Australia Final Report FRDC Project 2008/012 ISBN 978-0-646-53636-1 Jan-Olaf Meynecke, Shing Yip Lee, Mark Grubert, Ian Brown, Steven Montgomery, Neil Gribble, Danielle Johnston, Jonathan Gillson 180 Robinson and Wearyan river 900 160 800 140 700 120 600 l l 100 500 a f UE n 80 400 i CP ra 60 300 40 200 20 100 0 0 1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 2006 2007 2008 FINAL REPORT FRDC 2008/012 [ISBN 978-0-646-53636-1] ii EVALUATING THE ENVIRONMENTAL DRIVERS OF MUD CRAB (SCYLLA SERRATA) CATCHES IN AUSTRALIA FINAL REPORT FRDC 2008/012 Evaluating the environmental drivers of mud crab (Scylla serrata) catches in Australia July 2010 Enquiries should be addressed to: Jan-Olaf Meynecke [email protected] Copyright and Disclaimer © 2010 Fisheries Research and Development Corporation and Griffith University Except as permitted under the Copyright Act 1968 (Cth), no part of this publication may be reproduced by any process, electronic or otherwise, without the specific written permission of the copyright owners. Information may not be stored electronically in any form whatsoever without such permission. Important Disclaimer The authors do not warrant that the information in this document is free from errors or omissions. The authors do not accept any form of liability, be it contractual, tortious, or otherwise, for the contents of this document or for any consequences arising from its use or any reliance placed upon it.
    [Show full text]
  • Assessment of the Blue Swimmer Crab (Portunus Armatus) Fishery in Queensland
    Assessment of the blue swimmer crab (Portunus armatus) fishery in Queensland This publication has been compiled by Wayne Sumpton, Matthew Campbell, Michael O’Neill, Mark McLennan, Alex Campbell and George Leigh of Sustainable Fisheries, Department of Agriculture and Fisheries, and You-Gan Wang and Luke Lloyd-Jones of the University of Queensland. © State of Queensland, 2015. The Queensland Government supports and encourages the dissemination and exchange of its information. The copyright in this publication is licensed under a Creative Commons Attribution 3.0 Australia (CC BY) licence. Under this licence you are free, without having to seek our permission, to use this publication in accordance with the licence terms. You must keep intact the copyright notice and attribute the State of Queensland as the source of the publication. Note: Some content in this publication may have different licence terms as indicated. For more information on this licence, visit http://creativecommons.org/licenses/by/3.0/au/deed.en The information contained herein is subject to change without notice. The Queensland Government shall not be liable for technical or other errors or omissions contained herein. The reader/user accepts all risks and responsibility for losses, damages, costs and other consequences resulting directly or indirectly from using this information Table of Contents 1. INTRODUCTION AND BACKGROUND ................................................................... 13 HISTORICAL DEVELOPMENT OF THE BLUE SWIMMER CRAB FISHERY IN QUEENSLAND..................................
    [Show full text]
  • Crabs, Holothurians, Sharks, Batoid Fishes, Chimaeras, Bony Fishes, Estuarine Crocodiles, Sea Turtles, Sea Snakes, and Marine Mammals
    FAOSPECIESIDENTIFICATIONGUIDEFOR FISHERYPURPOSES ISSN1020-6868 THELIVINGMARINERESOURCES OF THE WESTERNCENTRAL PACIFIC Volume2.Cephalopods,crustaceans,holothuriansandsharks FAO SPECIES IDENTIFICATION GUIDE FOR FISHERY PURPOSES THE LIVING MARINE RESOURCES OF THE WESTERN CENTRAL PACIFIC VOLUME 2 Cephalopods, crustaceans, holothurians and sharks edited by Kent E. Carpenter Department of Biological Sciences Old Dominion University Norfolk, Virginia, USA and Volker H. Niem Marine Resources Service Species Identification and Data Programme FAO Fisheries Department with the support of the South Pacific Forum Fisheries Agency (FFA) and the Norwegian Agency for International Development (NORAD) FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS Rome, 1998 ii The designations employed and the presentation of material in this publication do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers and boundaries. M-40 ISBN 92-5-104051-6 All rights reserved. No part of this publication may be reproduced by any means without the prior written permission of the copyright owner. Applications for such permissions, with a statement of the purpose and extent of the reproduction, should be addressed to the Director, Publications Division, Food and Agriculture Organization of the United Nations, via delle Terme di Caracalla, 00100 Rome, Italy. © FAO 1998 iii Carpenter, K.E.; Niem, V.H. (eds) FAO species identification guide for fishery purposes. The living marine resources of the Western Central Pacific. Volume 2. Cephalopods, crustaceans, holothuri- ans and sharks. Rome, FAO. 1998. 687-1396 p.
    [Show full text]
  • Discovery of an Alien Crab, Scylla Serrata (Forsskål, 1775) (Crustacea: Decapoda: Portunidae), from the Caribbean Coast of Colombia
    BioInvasions Records (2013) Volume 2, Issue 4: 311–315 Open Access doi: http://dx.doi.org/10.3391/bir.2013.2.4.08 © 2013 The Author(s). Journal compilation © 2013 REABIC Rapid Communication Discovery of an alien crab, Scylla serrata (Forsskål, 1775) (Crustacea: Decapoda: Portunidae), from the Caribbean coast of Colombia Rafael Lemaitre1*, Néstor H. Campos2, Efraín A. Viloria Maestre3 and Amanda M. Windsor1 1 Department of Invertebrate Zoology, National Museum of Natural History, Smithsonian Institution, 4210 Silver Hill Road, Suitland, MD 20746 USA 2 Sede Caribe, Universidad Nacional de Colombia, c/o INVEMAR, Calle 25 # 2 - 55, Rodadero sur, Playa Salguero, Santa Marta, Colombia 3 Efraín A. Viloria Maestre, Programa Valoración y Aprovechamiento de Recursos Marinos, INVEMAR, Calle 25 # 2 - 55, Rodadero sur, Playa Salguero, Santa Marta, Colombia E-mail: [email protected] (RL), [email protected] (NHC), [email protected] (EAVM), [email protected] (AMW) *Corresponding author Received: 13 September 2013 / Accepted: 14 November 2013 / Published online: 19 November 2013 Handling editor: Vadim Panov Abstract A specimen of the swimming crab Scylla serrata, a species native to the tropical Indo-West and South Pacific, is reported from Cartagena Bay, Colombia, in the southern Caribbean. It is the third alien decapod crustacean documented from the Caribbean coast of Colombia. Analyses of genetic sequences points to a northern Indian Ocean origin of this specimen. Presence of this specimen is considered an isolated case, most likely kept alive for human consumption on board ship and discarded in the Bay. Key words: Scylla serrata; “giant mud crab”; alien marine species; Crustacea; Brachyura; Indo-West Pacific; western Atlantic; southern Caribbean; Colombia Introduction was collected in the Bay of Bengal, Indian Ocean, from a depth of 82 m.
    [Show full text]
  • Evaluation of Selected Commercial Aquaculture Feeds As Substitute for Natural Feeds in Rearing Mud Crab (Scylla Serrata) Juveniles Jerome G
    Evaluation of selected commercial aquaculture feeds as substitute for natural feeds in rearing mud crab (Scylla serrata) juveniles Jerome G. Genodepa, Alan N. Failaman Institute of Aquaculture, College of Fisheries and Ocean Sciences, University of the Philippines Visayas, Miag-ao, Iloilo 5023, Philippines. Corresponding author: J. Genodepa, [email protected] Abstract. A feeding trial was conducted to assess the potential of commercial feeds for fish and shrimp as substitute for natural feeds in rearing juveniles of the mud crab, Scylla serrata. The crablets (2nd crab instar; 0.044 to 0.049 g average body weight (ABW); n = 300) were fed either fish feed, shrimp feed or natural feeds (mussel and Acetes) alone, or with the 50-50% combination of the natural feed and each of the commercial feeds. The performance of the feeds and their combinations were evaluated after 11 and 20 days of rearing in 1000 L tanks based on survival, ABW, specific growth rate (SGR) and degree of aggression (indicated by missing appendages). The commercial diets tested were not suitable as complete replacement for natural feeds in rearing mud crab juveniles. Treatments fed with the commercial diets had significantly lower survival (35 to 54%) and ABW (0.186 to 0.399 g) compared to the treatment fed natural feeds alone (74% survival and 0.506 g ABW) after 20 days (p < 0.01). The combination of commercial feeds and natural feeds can possibly replace natural feeds during nursery rearing of juvenile crabs. The ABW in the treatment fed the combination of shrimp feed and natural feeds (0.611 g) was significantly higher (p < 0.01) than the treatment fed natural feeds only (0.506 g) while it did not differ between the treatment fed natural feeds only and the combination of fish feed and natural feeds (0.535g).
    [Show full text]
  • Temperature Potentially Induced Distinctive Flavor of Mud Crab Scylla Paramamosain Mediated by Gut Microbiota
    www.nature.com/scientificreports OPEN Temperature potentially induced distinctive favor of mud crab Scylla paramamosain mediated by gut microbiota Lei Tang1, Huan Wang 1,2*, Chunlin Wang1,2, Changkao Mu1,2, Hongling Wei1, Hongzhi Yao1, Chunyu Ye3,4, Lizhi Chen3,4 & Ce Shi1,2 Many factors afect the favor of crabs. However, impact of temperature on favor has not been reported. Here, we examined Scylla paramamosain collected within the main four producing areas in China from north sampling point (NP) and south sampling point (SP), respectively. The contents of favouring-related substances in hepatopancreas, muscles and gonads were determined by high- performance liquid chromatography (HPLC). Meanwhile, high-throughput sequencing of 16S RNA gene was used to reveal the diversity distribution of gut microbiota at each sample collection point. Comparisons among favor substances of edible parts, the implied higher temperature in SP may be benefcial to the accumulation of favor substances in gonads, while lower temperature in NP may be benefcial to the accumulation of favor substances in muscles and hepatopancreas. The gut microbiota of crabs, was analyzed via 16S rRNA gene sequencing. The results of gut microbiota showed that there were signifcant diferences in the distribution of gut microbiota in NP and SP. The microbiota composition of SP has a high distribution richness and no absolute dominant bacteria, while NP has absolute dominant bacteria and its microbiota richness was lower than SP. The results of redundancy analysis (RDA) showed that there was a signifcant correlation between temperature and the relative abundance of gut microbiota, and a signifcant correlation between gut microbiota and the content of favor substances.
    [Show full text]