DOCSLIB.ORG

RPL8 Antibody Cat

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
RPL8 Antibody Cat RPL8 Antibody Cat. No.: 57-687 RPL8 Antibody Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human Predicted species reactivity based on immunogen sequence: Bovine, Zebrafish, Mouse, HOMOLOGY: Rat, Xenopus This RPL8 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 207-236 amino acids from the C-terminal region of human RPL8. TESTED APPLICATIONS: WB APPLICATIONS: For WB starting dilution is: 1:1000 PREDICTED MOLECULAR 28 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig September 30, 2021 1 https://www.prosci-inc.com/rpl8-antibody-57-687.html CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: RPL8 ALTERNATE NAMES: 60S ribosomal protein L8, RPL8 ACCESSION NO.: P62917 PROTEIN GI NO.: 51702823 GENE ID: 6132 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the BACKGROUND: 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]. REFERENCES: 1) Salas, S., et al. Int. J. Cancer 125(4):851-860(2009) 2) Stelzl, U., et al. Cell 122(6):957-968(2005) 3) Andersen, J.S., et al. Nature 433(7021):77-83(2005) 4) Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005) ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 30, 2021 2 https://www.prosci-inc.com/rpl8-antibody-57-687.html.
Load more

Recommended publications
  • The Rise and Fall of the Bovine Corpus Luteum
    University of Nebraska Medical Center DigitalCommons@UNMC Theses & Dissertations Graduate Studies Spring 5-6-2017 The Rise and Fall of the Bovine Corpus Luteum Heather Talbott University of Nebraska Medical Center Follow this and additional works at: https://digitalcommons.unmc.edu/etd Part of the Biochemistry Commons, Molecular Biology Commons, and the Obstetrics and Gynecology Commons Recommended Citation Talbott, Heather, "The Rise and Fall of the Bovine Corpus Luteum" (2017). Theses & Dissertations. 207. https://digitalcommons.unmc.edu/etd/207 This Dissertation is brought to you for free and open access by the Graduate Studies at DigitalCommons@UNMC. It has been accepted for inclusion in Theses & Dissertations by an authorized administrator of DigitalCommons@UNMC. For more information, please contact [email protected]. THE RISE AND FALL OF THE BOVINE CORPUS LUTEUM by Heather Talbott A DISSERTATION Presented to the Faculty of the University of Nebraska Graduate College in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy Biochemistry and Molecular Biology Graduate Program Under the Supervision of Professor John S. Davis University of Nebraska Medical Center Omaha, Nebraska May, 2017 Supervisory Committee: Carol A. Casey, Ph.D. Andrea S. Cupp, Ph.D. Parmender P. Mehta, Ph.D. Justin L. Mott, Ph.D. i ACKNOWLEDGEMENTS This dissertation was supported by the Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture (NIFA) Pre-doctoral award; University of Nebraska Medical Center Graduate Student Assistantship; University of Nebraska Medical Center Exceptional Incoming Graduate Student Award; the VA Nebraska-Western Iowa Health Care System Department of Veterans Affairs; and The Olson Center for Women’s Health, Department of Obstetrics and Gynecology, Nebraska Medical Center.
    [Show full text]
  • Allele-Specific Expression of Ribosomal Protein Genes in Interspecific Hybrid Catfish
    Allele-specific Expression of Ribosomal Protein Genes in Interspecific Hybrid Catfish by Ailu Chen A dissertation submitted to the Graduate Faculty of Auburn University in partial fulfillment of the requirements for the Degree of Doctor of Philosophy Auburn, Alabama August 1, 2015 Keywords: catfish, interspecific hybrids, allele-specific expression, ribosomal protein Copyright 2015 by Ailu Chen Approved by Zhanjiang Liu, Chair, Professor, School of Fisheries, Aquaculture and Aquatic Sciences Nannan Liu, Professor, Entomology and Plant Pathology Eric Peatman, Associate Professor, School of Fisheries, Aquaculture and Aquatic Sciences Aaron M. Rashotte, Associate Professor, Biological Sciences Abstract Interspecific hybridization results in a vast reservoir of allelic variations, which may potentially contribute to phenotypical enhancement in the hybrids. Whether the allelic variations are related to the downstream phenotypic differences of interspecific hybrid is still an open question. The recently developed genome-wide allele-specific approaches that harness high- throughput sequencing technology allow direct quantification of allelic variations and gene expression patterns. In this work, I investigated allele-specific expression (ASE) pattern using RNA-Seq datasets generated from interspecific catfish hybrids. The objective of the study is to determine the ASE genes and pathways in which they are involved. Specifically, my study investigated ASE-SNPs, ASE-genes, parent-of-origins of ASE allele and how ASE would possibly contribute to heterosis. My data showed that ASE was operating in the interspecific catfish system. Of the 66,251 and 177,841 SNPs identified from the datasets of the liver and gill, 5,420 (8.2%) and 13,390 (7.5%) SNPs were identified as significant ASE-SNPs, respectively.
    [Show full text]
  • Attachment PDF Icon
    Spectrum Name of Protein Count of Peptides Ratio (POL2RA/IgG control) POLR2A_228kdBand POLR2A DNA-directed RNA polymerase II subunit RPB1 197 NOT IN CONTROL IP POLR2A_228kdBand POLR2B DNA-directed RNA polymerase II subunit RPB2 146 NOT IN CONTROL IP POLR2A_228kdBand RPAP2 Isoform 1 of RNA polymerase II-associated protein 2 24 NOT IN CONTROL IP POLR2A_228kdBand POLR2G DNA-directed RNA polymerase II subunit RPB7 23 NOT IN CONTROL IP POLR2A_228kdBand POLR2H DNA-directed RNA polymerases I, II, and III subunit RPABC3 19 NOT IN CONTROL IP POLR2A_228kdBand POLR2C DNA-directed RNA polymerase II subunit RPB3 17 NOT IN CONTROL IP POLR2A_228kdBand POLR2J RPB11a protein 7 NOT IN CONTROL IP POLR2A_228kdBand POLR2E DNA-directed RNA polymerases I, II, and III subunit RPABC1 8 NOT IN CONTROL IP POLR2A_228kdBand POLR2I DNA-directed RNA polymerase II subunit RPB9 9 NOT IN CONTROL IP POLR2A_228kdBand ALMS1 ALMS1 3 NOT IN CONTROL IP POLR2A_228kdBand POLR2D DNA-directed RNA polymerase II subunit RPB4 6 NOT IN CONTROL IP POLR2A_228kdBand GRINL1A;Gcom1 Isoform 12 of Protein GRINL1A 6 NOT IN CONTROL IP POLR2A_228kdBand RECQL5 Isoform Beta of ATP-dependent DNA helicase Q5 3 NOT IN CONTROL IP POLR2A_228kdBand POLR2L DNA-directed RNA polymerases I, II, and III subunit RPABC5 5 NOT IN CONTROL IP POLR2A_228kdBand KRT6A Keratin, type II cytoskeletal 6A 3 NOT IN CONTROL IP POLR2A_228kdBand POLR2K DNA-directed RNA polymerases I, II, and III subunit RPABC4 2 NOT IN CONTROL IP POLR2A_228kdBand RFC4 Replication factor C subunit 4 1 NOT IN CONTROL IP POLR2A_228kdBand RFC2
    [Show full text]
  • The VE-Cadherin/Amotl2 Mechanosensory Pathway Suppresses Aortic InAmmation and the Formation of Abdominal Aortic Aneurysms
    The VE-cadherin/AmotL2 mechanosensory pathway suppresses aortic inammation and the formation of abdominal aortic aneurysms Yuanyuan Zhang Karolinska Institute Evelyn Hutterer Karolinska Institute Sara Hultin Karolinska Institute Otto Bergman Karolinska Institute Maria Forteza Karolinska Institute Zorana Andonovic Karolinska Institute Daniel Ketelhuth Karolinska University Hospital, Stockholm, Sweden Joy Roy Karolinska Institute Per Eriksson Karolinska Institute Lars Holmgren ( [email protected] ) Karolinska Institute Article Keywords: arterial endothelial cells (ECs), vascular disease, abdominal aortic aneurysms Posted Date: June 15th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-600069/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License The VE-cadherin/AmotL2 mechanosensory pathway suppresses aortic inflammation and the formation of abdominal aortic aneurysms Yuanyuan Zhang1, Evelyn Hutterer1, Sara Hultin1, Otto Bergman2, Maria J. Forteza2, Zorana Andonovic1, Daniel F.J. Ketelhuth2,3, Joy Roy4, Per Eriksson2 and Lars Holmgren1*. 1Department of Oncology-Pathology, BioClinicum, Karolinska Institutet, Stockholm, Sweden. 2Department of Medicine Solna, BioClinicum, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden. 3Department of Cardiovascular and Renal Research, Institutet of Molecular Medicine, Univ. of Southern Denmark, Odense, Denmark 4Department of Molecular Medicine and Surgery, Karolinska Institutet, Karolinska University Hospital, Stockholm,
    [Show full text]
  • Inflammation Leads to Distinct Populations of Extracellular Vesicles from Microglia Yiyi Yang1* , Antonio Boza-Serrano1, Christopher J
    Yang et al. Journal of Neuroinflammation (2018) 15:168 https://doi.org/10.1186/s12974-018-1204-7 RESEARCH Open Access Inflammation leads to distinct populations of extracellular vesicles from microglia Yiyi Yang1* , Antonio Boza-Serrano1, Christopher J. R. Dunning2, Bettina Hjelm Clausen3,4, Kate Lykke Lambertsen3,4,5 and Tomas Deierborg1* Abstract Background: Activated microglia play an essential role in inflammatory responses elicited in the central nervous system (CNS). Microglia-derived extracellular vesicles (EVs) are suggested to be involved in propagation of inflammatory signals and in the modulation of cell-to-cell communication. However, there is a lack of knowledge on the regulation of EVs and how this in turn facilitates the communication between cells in the brain. Here, we characterized microglial EVs under inflammatory conditions and investigated the effects of inflammation on the EV size, quantity, and protein content. Methods: We have utilized western blot, nanoparticle tracking analysis (NTA), and mass spectrometry to characterize EVs and examine the alterations of secreted EVs from a microglial cell line (BV2) following lipopolysaccharide (LPS) and tumor necrosis factor (TNF) inhibitor (etanercept) treatments, or either alone. The inflammatory responses were measured with multiplex cytokine ELISA and western blot. We also subjected TNF knockout mice to experimental stroke (permanent middle cerebral artery occlusion) and validated the effect of TNF inhibition on EV release. Results: Our analysis of EVs originating from activated BV2 microglia revealed a significant increase in the intravesicular levels of TNF and interleukin (IL)-6. We also observed that the number of EVs released was reduced both in vitro and in vivo when inflammation was inhibited via the TNF pathway.
    [Show full text]
  • Supplementary Table 1. the List of Proteins with at Least 2 Unique
    Supplementary table 1. The list of proteins with at least 2 unique peptides identified in 3D cultured keratinocytes exposed to UVA (30 J/cm2) or UVB irradiation (60 mJ/cm2) and treated with treated with rutin [25 µM] or/and ascorbic acid [100 µM]. Nr Accession Description 1 A0A024QZN4 Vinculin 2 A0A024QZN9 Voltage-dependent anion channel 2 3 A0A024QZV0 HCG1811539 4 A0A024QZX3 Serpin peptidase inhibitor 5 A0A024QZZ7 Histone H2B 6 A0A024R1A3 Ubiquitin-activating enzyme E1 7 A0A024R1K7 Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein 8 A0A024R280 Phosphoserine aminotransferase 1 9 A0A024R2Q4 Ribosomal protein L15 10 A0A024R321 Filamin B 11 A0A024R382 CNDP dipeptidase 2 12 A0A024R3V9 HCG37498 13 A0A024R3X7 Heat shock 10kDa protein 1 (Chaperonin 10) 14 A0A024R408 Actin related protein 2/3 complex, subunit 2, 15 A0A024R4U3 Tubulin tyrosine ligase-like family 16 A0A024R592 Glucosidase 17 A0A024R5Z8 RAB11A, member RAS oncogene family 18 A0A024R652 Methylenetetrahydrofolate dehydrogenase 19 A0A024R6C9 Dihydrolipoamide S-succinyltransferase 20 A0A024R6D4 Enhancer of rudimentary homolog 21 A0A024R7F7 Transportin 2 22 A0A024R7T3 Heterogeneous nuclear ribonucleoprotein F 23 A0A024R814 Ribosomal protein L7 24 A0A024R872 Chromosome 9 open reading frame 88 25 A0A024R895 SET translocation 26 A0A024R8W0 DEAD (Asp-Glu-Ala-Asp) box polypeptide 48 27 A0A024R9E2 Poly(A) binding protein, cytoplasmic 1 28 A0A024RA28 Heterogeneous nuclear ribonucleoprotein A2/B1 29 A0A024RA52 Proteasome subunit alpha 30 A0A024RAE4 Cell division cycle 42 31
    [Show full text]
  • Inhibition of the MID1 Protein Complex
    Matthes et al. Cell Death Discovery (2018) 4:4 DOI 10.1038/s41420-017-0003-8 Cell Death Discovery ARTICLE Open Access Inhibition of the MID1 protein complex: a novel approach targeting APP protein synthesis Frank Matthes1,MoritzM.Hettich1, Judith Schilling1, Diana Flores-Dominguez1, Nelli Blank1, Thomas Wiglenda2, Alexander Buntru2,HannaWolf1, Stephanie Weber1,InaVorberg 1, Alina Dagane2, Gunnar Dittmar2,3,ErichWanker2, Dan Ehninger1 and Sybille Krauss1 Abstract Alzheimer’s disease (AD) is characterized by two neuropathological hallmarks: senile plaques, which are composed of amyloid-β (Aβ) peptides, and neurofibrillary tangles, which are composed of hyperphosphorylated tau protein. Aβ peptides are derived from sequential proteolytic cleavage of the amyloid precursor protein (APP). In this study, we identified a so far unknown mode of regulation of APP protein synthesis involving the MID1 protein complex: MID1 binds to and regulates the translation of APP mRNA. The underlying mode of action of MID1 involves the mTOR pathway. Thus, inhibition of the MID1 complex reduces the APP protein level in cultures of primary neurons. Based on this, we used one compound that we discovered previously to interfere with the MID1 complex, metformin, for in vivo experiments. Indeed, long-term treatment with metformin decreased APP protein expression levels and consequently Aβ in an AD mouse model. Importantly, we have initiated the metformin treatment late in life, at a time-point where mice were in an already progressed state of the disease, and could observe an improved behavioral phenotype. These 1234567890 1234567890 findings together with our previous observation, showing that inhibition of the MID1 complex by metformin also decreases tau phosphorylation, make the MID1 complex a particularly interesting drug target for treating AD.
    [Show full text]
  • (HBV) Infection of the Liver Ahmed Mohamed Abdel-B Diab Purdue University
    Purdue University Purdue e-Pubs Open Access Dissertations Theses and Dissertations January 2016 The oler of PLK1 in Hepatitis B Virus (HBV) infection of the liver Ahmed Mohamed Abdel-B Diab Purdue University Follow this and additional works at: https://docs.lib.purdue.edu/open_access_dissertations Recommended Citation Diab, Ahmed Mohamed Abdel-B, "The or le of PLK1 in Hepatitis B Virus (HBV) infection of the liver" (2016). Open Access Dissertations. 1214. https://docs.lib.purdue.edu/open_access_dissertations/1214 This document has been made available through Purdue e-Pubs, a service of the Purdue University Libraries. Please contact [email protected] for additional information. Graduate School Form 30 Updated 12/26/2015 PURDUE UNIVERSITY GRADUATE SCHOOL Thesis/Dissertation Acceptance This is to certify that the thesis/dissertation prepared By Ahmed Mohamed Abdel-B Diab Entitled The role of PLK1 in Hepatitis B Virus (HBV) Infection of the Liver For the degree of Doctor of Philosophy Is approved by the final examining committee: Ourania Andrisani Andy W. Tao Co-chair Fabien Zoulim Co-chair Robert Geahlen Xiaoqi Liu To the best of my knowledge and as understood by the student in the Thesis/Dissertation Agreement, Publication Delay, and Certification Disclaimer (Graduate School Form 32), this thesis/dissertation adheres to the provisions of Purdue University’s “Policy of Integrity in Research” and the use of copyright material. Approved by Major Professor(s): Ourania Andrisani Laurie Jaeger 10/6/2016 Approved by: Head of the Departmental Graduate Program Date i THE ROLE OF PLK1 IN HEPATITIS B VIRUS (HBV) INFECTION OF THE LIVER A Dissertation Submitted to the Faculty of Purdue University by Ahmed M Diab In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy December 2016 Purdue University West Lafayette, Indiana ii ACKNOWLEDGEMENTS I would like to begin by extending my gratitude to my committee members Robert Geahlen, Andy W.
    [Show full text]
  • Hypoxia-Inducible-Factor Dependent Induction of Netrin-1
    bioRxiv preprint doi: https://doi.org/10.1101/561340; this version posted May 15, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Circadian light-mediated endothelial metabolic reprogramming Yoshimasa Oyama MD, Ph.D.,1* Colleen M. Bartman Ph.D.,1,4* Stephanie Bonney Ph.D.,1,4* J. Scott Lee Ph.D.,1 Lori A. Walker Ph.D.,2 Jun Han Ph.D.,3 Christoph H. Borchers Ph.D.,3 Peter M. Buttrick M.D.,2 Carol M. Aherne Ph.D.1, Nathan Clendenen M.D.,1 Sean P. Colgan Ph.D.1 and Tobias Eckle M.D., Ph.D.1,2,4# *authors contributed equally 1Mucosal Inflammation Program, Departments of Medicine and Anesthesiology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA; 2Division of Cardiology, Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, USA, 3Department of Biochemistry and Microbiology, Genome BC Proteomics Centre, University of Victoria, Victoria, BC, Canada; 4Graduate Training Program in Cell Biology, Stem Cells, and Development, University of Colorado Anschutz Medical Campus, Aurora, CO, USA #Correspondence should be addressed to: Tobias Eckle, M.D., Ph.D. Professor of Anesthesiology, Cardiology and Cell Biology Department of Anesthesiology University of Colorado Denver 12700 E 19th Avenue, Mailstop B112, RC 2, Room 7121 Aurora, CO 80045; Office: +1-303-724 -2932 or - 2947, Fax: +1-303-724-2852 Email: [email protected] Short title: Intense light as endothelium-targeting strategy in myocardial ischemia bioRxiv preprint doi: https://doi.org/10.1101/561340; this version posted May 15, 2019.
    [Show full text]
  • Anticancer Effects of Β-Elemene in Gastric Cancer Cells and Its Potential Underlying Proteins: a Proteomic Study
    ONCOLOGY REPORTS 32: 2635-2647, 2014 Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study JUN-SONG LIU, SHI-CAI HE, ZHENG-LIANG ZHANG, RUI CHEN, LIN FAN, GUANG-LIN QIU, SHUAI CHANG, LIANG LI and XIANG-MING CHE Department of General Surgery, The First Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China Received July 4, 2014; Accepted August 26, 2014 DOI: 10.3892/or.2014.3490 Abstract. Gastric cancer is a common malignancy with a Introduction poor prognosis. β-elemene is a broad-spectrum anticancer drug extracted from the traditional Chinese medicinal herb Gastric cancer is the fourth most common malignancy in Curcuma wenyujin. In the present study, we investigated the the world and the second leading cause of cancer-related anticancer effects of β-elemene in gastric cancer cells and the mortality (1). At present, surgical resection remains the potential proteins involved. Human SGC7901 and MKN45 main therapeutic strategy for gastric cancer, supplemented gastric cancer cells were treated with different concentrations with perioperative chemotherapy, chemoradiotherapy and/or of β-elemene. Cell viability, clonogenic survival and apoptotic immunotherapy (2-5). However, most patients are diagnosed cell death were assessed. β-elemene inhibited viability and with advanced gastric cancer which may have progressed decreased clonogenic survival of gastric cancer cells in a beyond the curative potential of surgical operation (6,7). In dose-dependent manner. Apoptosis induction contributed to addition, previous studies have demonstrated that a consid- the anticancer effects. We then employed a proteomic method, erable proportion of patients receiving potentially curative isobaric tags for relative and absolute quantitation (iTRAQ), to resection experienced recurrences which lead to unfavorable detect the proteins altered by β-elemene.
    [Show full text]
  • Protein List
    Protein Accession Protein Id Protein Name P11171 41 Protein 4.
    [Show full text]
  • Ribosomal Protein Mutants and Their Effects on Plant Growth and Development
    RIBOSOMAL PROTEIN MUTANTS AND THEIR EFFECTS ON PLANT GROWTH AND DEVELOPMENT A Thesis Submitted to the College of Graduate Studies and Research In Partial Fulfillment of the Requirements For the Degree of Doctor of Philosophy In the Department of Biology University of Saskatchewan Saskatoon By Chad Dale Stewart © Copyright Chad Stewart, November, 2012. All rights reserved. PERMISSION TO USE In presenting this thesis in partial fulfilment of the requirements for a Postgraduate degree from the University of Saskatchewan, I agree that the Libraries of this University may make it freely available for inspection. I further agree that permission for copying of this thesis in any manner, in whole or in part, for scholarly purposes may be granted by the professor or professors who supervised my thesis work or, in their absence, by the Head of the Department or the Dean of the College in which my thesis work was done. It is understood that any copying or publication or use of this thesis or parts thereof for financial gain shall not be allowed without my written permission. It is also understood that due recognition shall be given to me and to the University of Saskatchewan in any scholarly use which may be made of any material in my thesis. Requests for permission to copy or to make other use of material in this thesis in whole or part should be addressed to: Head of the Department of Biology University of Saskatchewan Saskatoon, Saskatchewan S7N 5E2 i ABSTRACT Ribosomes, large enzymatic complexes containing an RNA catalytic core, drive protein synthesis in all living organisms.
    [Show full text]