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Notes on Powdery Mildews (Erysiphales) in Japan: III See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/264624638 Notes on powdery mildews (Erysiphales) in Japan: III. Golovinomyces and Podosphaera Article in Mycoscience · March 2015 DOI: 10.1016/j.myc.2014.07.002 CITATIONS READS 6 1,522 2 authors: Jamjan Meeboon Susumu Takamatsu National Agriculture and Food Research Organization Mie University 58 PUBLICATIONS 525 CITATIONS 229 PUBLICATIONS 5,547 CITATIONS SEE PROFILE SEE PROFILE All content following this page was uploaded by Susumu Takamatsu on 21 November 2017. The user has requested enhancement of the downloaded file. mycoscience 56 (2015) 243e251 Available online at www.sciencedirect.com journal homepage: www.elsevier.com/locate/myc Short communication Notes on powdery mildews (Erysiphales) in Japan: III. Golovinomyces and Podosphaera * Jamjan Meeboon a,b, Susumu Takamatsu a, a Department of Bioresources, Graduate School, Mie University, 1577 Kurima-Machiya, Tsu 514-8507, Japan b Department of Agriculture, Ministry of Agriculture and Cooperatives, 50 Phaholyothin Rd., Ladyao, Chatuchak, Bangkok, Thailand article info abstract Article history: Several new records of Golovinomyces and Podosphaera species (Erysiphales) in Japan are Received 22 May 2014 reported with morphological and molecular data, including the first finding of G. cynoglossi Received in revised form in Japan, the first occurrence of Euoidium sp. on Trigonotis peduncularis, G. riedlianus on 27 June 2014 Galium trifloriforme var. nipponicum and P. xanthii on Verbena brasiliensis, as well as the first Accepted 4 July 2014 morphological descriptions and molecular data for G. rubiae on Rubia hexaphylla, P. Available online 10 August 2014 curvispora on Aria alnifolia, P. tridactyla on Cerasus leveilleana, and P. xanthii on Parasenecio hastatus subsp. tanakae and V. bonariensis based on Japanese specimens. Keywords: © 2014 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved. Erysiphaceae Euoidium Molecular phylogeny Morphology New host This is the third part of a series of studies on powdery mildews Erysiphaceae that were retrieved from DNA databases (DDBJ, in Japan. In this report, we described and illustrated four EMBL, NCBI) using MUSCLE (Edgar 2004) implemented in powdery mildew species belonging to Golovinomyces (including MEGA 5 (Tamura et al. 2011). Alignments were then manually Euoidium anamorphs) and three species belonging to refined using the MEGA 5 program and were deposited in Podosphaera supplemented by molecular data. TreeBASE (http://www.treebase.org/) under the accession The nucleotide sequences of the 50-end of the 28S rRNA number S15835. Phylogenetic trees were obtained from the gene (including domains D1 and D2) and the internal tran- data using maximum parsimony (MP) method and maximum scribed spacer (ITS) regions were determined according to the likelihood (ML) method as described in Meeboon and procedure of Meeboon and Takamatsu (2015). Representative Takamatsu (2015). new sequences determined in this study were deposited in The 28S rRNA gene for three specimens of Podosphaera was DNA Data Base of Japan (DDBJ) under the accession numbers determined in this study. The three sequences were aligned AB936274eAB936277. Sequences generated from the 28S rRNA with 23 Podosphaera and Cystotheca sequences that were gene and ITS region were aligned with other sequences of the retrieved from the DNA databases. Two Cystotheca sequences * Corresponding author. Tel.: þ81 59 231 9497; fax: þ81 59 231 9540. E-mail address: [email protected] (S. Takamatsu). http://dx.doi.org/10.1016/j.myc.2014.07.002 1340-3540/© 2014 The Mycological Society of Japan. Published by Elsevier B.V. All rights reserved. 244 mycoscience 56 (2015) 243e251 Podosphaera xanthii ex Cucurbita AB462766 28S rRNA gene 26 sequences Podosphaera xanthii ex Hibiscus AB936276 680 characters 71 steps Podosphaera xanthii ex Verbena brasiliensis AB936277 CI = 0.803 RI = 0.844 Podosphaera xanthii ex Helianthus AB462781 RC = 0.678 Podosphaera xanthii ex Zinnia AB462782 Podosphaera xanthii ex Zehneria AB462792 Podosphaera balsaminae ex Impatiens AB462788 Podosphaera sp. ex Solanum AB462769 Podosphaera euphorbiae-hirtae ex Acalypha AB462770 Podosphaera sp. ex Vigna AB462784 -/72 Podosphaera xanthii ex Lactuca AB462773 Podosphaera sp. ex Verbena AB462780 Podosphaera xanthii ex Parasenecio AB936274 Podosphaera xanthii ex Gerbera AB462763 -/71 Podosphaera xanthii ex Cosmos AB462797 Podosphaera balsaminae ex Impatiens AB462787 Podosphaera elsholtziae ex Ajuga AB462794 91/94 Podosphaera erigerontis-canadensis ex Conyza AB462772 Podosphaera intermedia ex Clerodendrum AB462777 -/80 Podosphaera astericola ex Aster AB462779 Podosphaera astericola ex Aster AB462760 100/100 Podosphaera tridactyla ex Cerasus leveilleana AB936275 Podosphaera longiseta ex Padus AB022423 92/97 Podosphaera tridactyla ex Microcerasus AB022393 Cystotheca wrightii ex Quercus AB022355 Cystotheca lanestris ex Quercus AB022353 1 change Fig. 1 e Phylogenetic analysis of the divergent domains D1 and D2 sequences of the 28S rRNA gene for 26 sequences from the genus Podosphaera and Cystotheca. This tree is a phylogram of one of the 26 most parsimonious trees with 71 steps, which was found using a heuristic search. Horizontal branch lengths are proportional to the number of substitutions that were inferred to have occurred along a particular branch of the tree. BS (>70%) values by the maximum parsimony (MP) and maximum likelihood (ML) methods were shown on the respective branch. Sequences from the collections reported in this study are shown in boldface. were used as outgroup taxa according to Mori et al. (2000). The sequences that were retrieved from the DNA databases. Two alignment data matrix consisted of 26 sequences and 680 Cystotheca sequences were used as outgroup taxa according to characters, of which 53 (7.8%) characters were variable and 37 Mori et al. (2000). The alignment data matrix consisted of 51 (5.4%) characters were informative for parsimony analysis. A sequences and 496 characters, of which 143 (28.8%) characters total of 26 equally parsimonious trees with 71 steps were were variable and 117 (23.6%) characters were informative for constructed by the MP analysis. One of the 26 trees with the parsimony analysis. A total of six equally parsimonious trees highest log likelihood value is shown in Fig. 1. For the ITS with 285 steps were constructed by the MP analysis. One of the analysis, the five sequences derived from the fungi reported in six trees with the highest log likelihood value is shown in this study were aligned with 46 Podosphaera and Cystotheca Fig. 2. Phylogenetic trees generated from ML analysis were mycoscience 56 (2015) 243e251 245 Podosphaera xanthii ex Gynostemma D84378 ITS Podosphaera xanthii ex Verbena AB046985 51 sequences Podosphaera xanthii ex Vigna AB040297 496 characters Podosphaera xanthii ex Cucurbita AB040315 285 steps Podosphaera xanthii ex Cucumis AB040323 CI = 0.712 Podosphaera sp. ex Saintpaulia AB040338 RI = 0.879 Podosphaera xanthii ex Zinnia AB040354 RC = 0.626 Podosphaera euphorbiae-hirtae ex Acalypha AB040306 Podosphaera xanthii ex Hibiscus AB936276 Podosphaera sp. ex Dunbaria AB040334 Podosphaera xanthii ex Rudbeckia AB040296 Podosphaera xanthii ex Petasites AB040307 Podosphaera xanthii ex Physalis AB040336 Podosphaera xanthii ex Verbena bonariensis AB462804 Podosphaera xanthii ex Verbena brasiliensis AB936277 Podosphaera sp. ex Cayratia AB026151 Podosphaera xanthii ex Lactuca AB040294 Podosphaera pseudofusca ex Fatoua AB040320 Podosphaera sp. ex Verbena AB040347 Podosphaera xanthii ex Lactuca AB040352 75/80 Podosphaera xanthii ex Cosmos AB040300 Podosphaera xanthii ex Carthamus AB040298 Podosphaera xanthii ex Parasenecio AB936274 Podosphaera xanthii ex Euryops DQ205330 Podosphaera xanthii ex Coreopsis EF442023 Podosphaera xanthii ex Calendula AB040317 Podosphaera xanthii ex Farfugium AB040346 94/98 Podosphaera sp. ex Peristrophe AB026135 Podosphaera astericola ex Aster AB040335 Podosphaera astericola ex Aster AB040353 100/100 Podosphaera erigerontis-canadensis ex Taraxacum AB046987 Podosphaera elsholtziae ex Ajuga AB026142 Podosphaera phtheirospermi ex Melampyrum AB040332 Podosphaera intermedia ex Clerodendrum AB026145 Podosphaera tridactyla ex Prunus sp. AY833659 98/98 Podosphaera tridactyla ex Cerasus leveilleana AB936275 Podosphaera Cerasus 75/- sp. ex AB026138 100/100 Podosphaera tridactyla ex Armeniaca AY833657 Podosphaera tridactyla ex Prunus AY833658 Podosphaera tridactyla ex Padus AY833652 Podosphaera tridactyla ex Microcerasus AB000943 Podosphaera longiseta ex Padus AB000945 Podosphaera leucotricha ex Malus AF073353 100/100 Podosphaera leucotricha ex Malus AB027231 76/72 Podosphaera sp. ex Photinia AB525934 Podosphaera curvispora ex Aria AB525928 76/95 100/100 Podosphaera clandestina ex Crataegus AB525931 Podosphaera clandestina ex Crataegus AB525930 Podosphaera photiniae ex Photinia AB026147 100/100 Cystotheca wrightii ex Quercus AB000932 Cystotheca lanestris ex Quercus AB000933 5 changes Fig. 2 e Phylogenetic analysis of the ITS region for 51 sequences from the genus Podosphaera and Cystotheca. This tree is a phylogram of one of the six most parsimonious trees with 285 steps, which was found using a heuristic search. Horizontal branch lengths are proportional to the number of substitutions that were inferred to have occurred along a particular branch of the tree. BS (>70%) values by the maximum parsimony (MP) and maximum likelihood (ML)
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