Nutritionally Variant Streptococci)
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The Oral Microbiome of Healthy Japanese People at the Age of 90
applied sciences Article The Oral Microbiome of Healthy Japanese People at the Age of 90 Yoshiaki Nomura 1,* , Erika Kakuta 2, Noboru Kaneko 3, Kaname Nohno 3, Akihiro Yoshihara 4 and Nobuhiro Hanada 1 1 Department of Translational Research, Tsurumi University School of Dental Medicine, Kanagawa 230-8501, Japan; [email protected] 2 Department of Oral bacteriology, Tsurumi University School of Dental Medicine, Kanagawa 230-8501, Japan; [email protected] 3 Division of Preventive Dentistry, Faculty of Dentistry and Graduate School of Medical and Dental Science, Niigata University, Niigata 951-8514, Japan; [email protected] (N.K.); [email protected] (K.N.) 4 Division of Oral Science for Health Promotion, Faculty of Dentistry and Graduate School of Medical and Dental Science, Niigata University, Niigata 951-8514, Japan; [email protected] * Correspondence: [email protected]; Tel.: +81-45-580-8462 Received: 19 August 2020; Accepted: 15 September 2020; Published: 16 September 2020 Abstract: For a healthy oral cavity, maintaining a healthy microbiome is essential. However, data on healthy microbiomes are not sufficient. To determine the nature of the core microbiome, the oral-microbiome structure was analyzed using pyrosequencing data. Saliva samples were obtained from healthy 90-year-old participants who attended the 20-year follow-up Niigata cohort study. A total of 85 people participated in the health checkups. The study population consisted of 40 male and 45 female participants. Stimulated saliva samples were obtained by chewing paraffin wax for 5 min. The V3–V4 hypervariable regions of the 16S ribosomal RNA (rRNA) gene were amplified by PCR. -
Prosthetic Joint Infection Caused by Granulicatella Adiacens
Quénard et al. BMC Musculoskeletal Disorders (2017) 18:276 DOI 10.1186/s12891-017-1630-1 CASEREPORT Open Access Prosthetic joint infection caused by Granulicatella adiacens: a case series and review of literature Fanny Quénard1, Piseth Seng1,2,3* , Jean-Christophe Lagier3, Florence Fenollar3 and Andreas Stein1,2,3 Abstract Background: Bone and joint infection involving Granulicatella adiacens is rare, and mainly involved in cases of bacteremia and infectious endocarditis. Here we report three cases of prosthetic joint infection involving G. adiacens that were successfully treated with surgery and prolonged antimicrobial treatment. We also review the two cases of prosthetic joint infection involving G. adiacens that are reported in the literature. Case presentation: Not all five cases of prosthetic joint infection caused by G. adiacens were associated with bacteremia or infectious endocarditis. Dental care before the onset of infection was observed in two cases. The median time delay between arthroplasty implantation and the onset of infection was of 4 years (ranging between 2 and 10 years). One of our cases was identified with 16srRNA gene sequencing, one case with MALDI-TOF mass spectrometry, and one case with both techniques. Two literature cases were diagnosed by 16srRNA gene sequencing. All five cases were cured after surgery including a two-stage prosthesis exchange in three cases, a one- stage prosthesis exchange in one case, and debridement, antibiotics, irrigation, and retention of the prosthesis in one case, and prolonged antimicrobial treatment. Conclusion: Prosthetic joint infection involving G. adiacens is probably often dismissed due to difficult culture or misdiagnosis, in particular in the cases of polymicrobial infection. -
Streptococcus Adjacens and Streptococcus Defectivus to Abiotrophia Gen
INTERNATIONAL JOURNALOF SYSTEMATIC BACTERIOLOGY, OCt. 1995, p. 798-803 Vol. 45, No. 4 0020-7713/95/$04.00 -t-0 Copyright 0 1995, International Union of Microbiological Societies Transfer of Streptococcus adjacens and Streptococcus defectivus to Abiotrophia gen. nov. as Abiotrophia adiacens comb. nov. and Abiotrophia defectiva comb. nov., Respectively YQSHIAKI KAWAMURA,” XIAO-GANG HOU, FERDOUSI SULTANA, SHUJUN LIU, HTROYUKI YAMAMOTO, AND TAKAYUKI EZAKI Department of Microbiology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu 500, Japan We performed this study to determine the 16s rRNA sequences of the type strains of Streptococcus adjacens and Streptococcus defectivus and to calculate the phylogenetic distances between these two nutritionally variant streptococci (NVS) and other members of the genus Streptococcus. S. adjacens and S. defectivus belonged to one cluster, but this cluster was not closely related to other streptococcal species. A comparative analysis of the sequences of these organisms and other low-G+C-content gram-positive bacteria revealed that the two NVS species formed a distinct cluster and were only remotely related to the Aerococcus and Carnobacterium clusters. The highest level of homology (93.7%) was found between S. adjacens and Carnobacterium divergens. Carnobac- terium species have meso-diaminopimelic acid in their cell walls, but S. adjacens and S. defectivus have L-lysine as the diamino acid at position 3 in their peptidoglycan tetrapeptides. On the basis of our findings and the results of previous phenotypic studies, we propose that the NVS species should be placed in a new genus, the genus Abiotrophia, as Abiotrophia adiacens comb. nov. and Abiotrophia defectiva comb. -
Granulicatella Adiacens Bacteria Isolation from Perodontitical
Sys Rev Pharm 2020; 11(4): 396 400 A multifaceted review journal in the field of pharmacy E-ISSN 0976-2779 P-ISSN 0975-8453 Granulicatella Adiacens Bacteria Isolation from Perodontitical Patients with Polymerase Chain Reaction Techniques Harun Achmad1, Sri Oktawati2,Andi Mardiana Adam2,Burhanuddin Pasiga3, Rizalinda Sjahril4, Aulia Azizah5, Bayu Indra Sukmana6, Huldani7, Heri Siswanto8 , Ingrid Neormansyah8 1Lecturer of Pedodontics Department, Dentistry Faculty, Hasanuddin University, Makassar, Indonesia 2Lecturer of Periodontology Department, Dentistry Faculty, Hasanuddin University, Makassar, Indonesia 3Lecturer of Dental Public Health Department, Dentistry Faculty, Hasanuddin University, Makassar, Indonesia 4 Lecturer of Microbiology Department, Medical Faculty, Hasanuddin University, Makassar, Indonesia 5Department of Preventive and Public Health Dentistry, Faculty of Dentistry, Lambung Mangkurat University, Banjarmasin, Indonesia. 6Department of Dental Radiology, Faculty of Dentistry, Lambung Mangkurat University, Banjarmasin, Indonesia. 7Department of Physiology, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia. 8 Resident of Periodontology Department, Dentistry Faculty, Hasanuddin University, Makassar, Indonesia Correspondence Author E-mail: [email protected] Article History: Submitted: 10.01.2020 Revised: 12.03.2020 Accepted: 24.04.2020 ABSTRACT Background: Periodontitis is an inflammatory disease of dental support the NCBI Gene Bank using BLAST analysis. tissue caused by certain groups of microorganisms, -
Abiotrophia Defectiva Liver Abscess in a Teenage Boy After a Supposedly Mild Blunt Abdominal Trauma: a Case Report Petar Rasic1* , Srdjan Bosnic1, Zorica V
Rasic et al. BMC Gastroenterology (2020) 20:267 https://doi.org/10.1186/s12876-020-01409-6 CASE REPORT Open Access Abiotrophia defectiva liver abscess in a teenage boy after a supposedly mild blunt abdominal trauma: a case report Petar Rasic1* , Srdjan Bosnic1, Zorica V. Vasiljevic2 , Slavisa M. Djuricic3,4 , Vesna Topic5, Maja Milickovic1,6 and Djordje Savic1,6 Abstract Background: A pyogenic liver abscess (PLA) represents a pus-filled cavity within the liver parenchyma caused by the invasion and multiplication of bacteria. The most common offender isolated from the PLA in children is Staphylococcus aureus. Abiotrophia defectiva is a Gram-positive pleomorphic bacterium, commonly found in the oral cavity, intestinal, and genitourinary mucosa as part of the normal microbiota. It has been proven to be an etiological factor in various infections, but rarely in cases of PLA. The case presented here is, to the best of our knowledge, the first pediatric case of PLA caused by A. defectiva. Case presentation: A 13-year-old Caucasian boy presented with a two-day history of abdominal pain, fever up to 40 °C, and polyuria. Contrast-enhanced computed tomography (CT) scan revealed a single, multiloculated liver lesion, suggestive of a liver abscess. The boy had sustained a bicycle handlebar injury to his upper abdomen 3 weeks before the symptoms appeared and had been completely asymptomatic until 2 days before admission. He was successfully treated with antibiotic therapy and open surgical drainage. A. defectiva was isolated from the abscess material. Histopathology report described the lesion as a chronic PLA. Conclusions: A. defectiva is a highly uncommon cause of liver abscess in children. -
Bacterial Diversity and Functional Analysis of Severe Early Childhood
www.nature.com/scientificreports OPEN Bacterial diversity and functional analysis of severe early childhood caries and recurrence in India Balakrishnan Kalpana1,3, Puniethaa Prabhu3, Ashaq Hussain Bhat3, Arunsaikiran Senthilkumar3, Raj Pranap Arun1, Sharath Asokan4, Sachin S. Gunthe2 & Rama S. Verma1,5* Dental caries is the most prevalent oral disease afecting nearly 70% of children in India and elsewhere. Micro-ecological niche based acidifcation due to dysbiosis in oral microbiome are crucial for caries onset and progression. Here we report the tooth bacteriome diversity compared in Indian children with caries free (CF), severe early childhood caries (SC) and recurrent caries (RC). High quality V3–V4 amplicon sequencing revealed that SC exhibited high bacterial diversity with unique combination and interrelationship. Gracillibacteria_GN02 and TM7 were unique in CF and SC respectively, while Bacteroidetes, Fusobacteria were signifcantly high in RC. Interestingly, we found Streptococcus oralis subsp. tigurinus clade 071 in all groups with signifcant abundance in SC and RC. Positive correlation between low and high abundant bacteria as well as with TCS, PTS and ABC transporters were seen from co-occurrence network analysis. This could lead to persistence of SC niche resulting in RC. Comparative in vitro assessment of bioflm formation showed that the standard culture of S. oralis and its phylogenetically similar clinical isolates showed profound bioflm formation and augmented the growth and enhanced bioflm formation in S. mutans in both dual and multispecies cultures. Interaction among more than 700 species of microbiota under diferent micro-ecological niches of the human oral cavity1,2 acts as a primary defense against various pathogens. Tis has been observed to play a signifcant role in child’s oral and general health. -
Atypica, and Granulicatella Adiacens in Biofilm of Complete Dentures
[Downloaded free from http://www.j-ips.org on Thursday, November 1, 2018, IP: 183.82.145.117] Original Article Relative presence of Streptococcus mutans, Veillonella atypica, and Granulicatella adiacens in biofilm of complete dentures Binoy Mathews Nedumgottil Department of Prosthodontics and Implantology, Mahe Institute of Dental Sciences and Hospital, Puducherry, India Abstract Aims and Objective: Oral biofilms in denture wearers are populated with a large number of bacteria, a few of which have been associated with medical conditions such as sepsis and infective endocarditis (IE). The present study was designed to investigate the relative presence of pathogenic bacteria in biofilms of denture wearers specifically those that are associated with IE. Methods: Biofilm samples from 88 denture wearers were collected and processed to extract total genomic DNA. Eight of these samples were subjected to 16S rRNA gene sequencing analysis to first identify the general bacterial occurrence pattern. This was followed by species-specific quantitative polymerase chain reaction (qPCR) on entire batch of 88 samples to quantify the relative copy numbers of IE-associated pathogens. Results: 16S rRNA gene analysis of eight biofilm samples identified bacteria from Firmicutes, Actinobacteria, Proteobacteria, Bacteroidetes, and Fusobacteria species. Interestingly, Streptococcus mutans, Veillonella atypica, and Granulicatella adiacens from Firmicutes, all known to be associated with early-onset sepsis and IE was present in five of eight biofilm samples. The other three samples carried bacteria from genus Proteobacteria with Neisseria flava and Neisseria mucosa, which are known to be commensals, as dominant species. Species-specific qPCR of S. mutans V. atypica, and G. adiacens on 88 biofilm DNA samples identified the presence of S. -
Identification and Antimicrobial Susceptibility of Granulicatella
Sys Rev Pharm 2020; 11(4): 324 331 A multifaceted review journal in the field of pharmacy E-ISSN 0976-2779 P-ISSN 0975-8453 Identification and Antimicrobial Susceptibility of Granulicatella adiacens Isolated from Periodontal Pocket *1Harun Achmad, 2Andi Mardiana Adam, 2Surijana Mappangara, 2Sri Oktawati, 3Rizalinda Sjahril, 1Marhamah F. Singgih, 2Ingrid Neormansyah, 2Heri Siswanto *1Pediatric Dentistry Department, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia 2Periodontology Department, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia 3Microbiology Department, Medical Faculty, Hasanuddin University, Makassar, Indonesia Correspondence Author E-mail: [email protected] Article History: Submitted: 23.01.2020 Revised: 20.03.2020 Accepted: 09.04.2020 ABSTRACT Background: Periodontal diseases often occur in developing and Granulicatella adiacens. Those samples were tested on seven types of developed countries, affecting 20 – 50% of the world population. antibiotic which is ofloxacin, ceftriaxone, azithromycin, vancomycin, Periodontitis may also complicate systemic health and have a risk to tetracycline, levofloxacin, and clindamycin. Sensitive isolates were cause endocarditis infection. Granulicatella adiacens, part found on 90% of ofloxacin and levofloxacin group, 85% of ofnutritionally variant streptococci (NVS), was proven to cause many azithromycin group, 50% of vancomycin group, and less than 50% for diseases, especially endocarditis infection. GA was a part of three other groups. High resistance -
Bacteriology
SECTION 1 High Yield Microbiology 1 Bacteriology MORGAN A. PENCE Definitions Obligate/strict anaerobe: an organism that grows only in the absence of oxygen (e.g., Bacteroides fragilis). Spirochete Aerobe: an organism that lives and grows in the presence : spiral-shaped bacterium; neither gram-positive of oxygen. nor gram-negative. Aerotolerant anaerobe: an organism that shows signifi- cantly better growth in the absence of oxygen but may Gram Stain show limited growth in the presence of oxygen (e.g., • Principal stain used in bacteriology. Clostridium tertium, many Actinomyces spp.). • Distinguishes gram-positive bacteria from gram-negative Anaerobe : an organism that can live in the absence of oxy- bacteria. gen. Bacillus/bacilli: rod-shaped bacteria (e.g., gram-negative Method bacilli); not to be confused with the genus Bacillus. • A portion of a specimen or bacterial growth is applied to Coccus/cocci: spherical/round bacteria. a slide and dried. Coryneform: “club-shaped” or resembling Chinese letters; • Specimen is fixed to slide by methanol (preferred) or heat description of a Gram stain morphology consistent with (can distort morphology). Corynebacterium and related genera. • Crystal violet is added to the slide. Diphtheroid: clinical microbiology-speak for coryneform • Iodine is added and forms a complex with crystal violet gram-positive rods (Corynebacterium and related genera). that binds to the thick peptidoglycan layer of gram-posi- Gram-negative: bacteria that do not retain the purple color tive cell walls. of the crystal violet in the Gram stain due to the presence • Acetone-alcohol solution is added, which washes away of a thin peptidoglycan cell wall; gram-negative bacteria the crystal violet–iodine complexes in gram-negative appear pink due to the safranin counter stain. -
Identification of Clinically Relevant Streptococcus and Enterococcus
pathogens Article Identification of Clinically Relevant Streptococcus and Enterococcus Species Based on Biochemical Methods and 16S rRNA, sodA, tuf, rpoB, and recA Gene Sequencing Maja Kosecka-Strojek 1,* , Mariola Wolska 1, Dorota Zabicka˙ 2 , Ewa Sadowy 3 and Jacek Mi˛edzobrodzki 1 1 Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland; [email protected] (M.W.); [email protected] (J.M.) 2 Department of Molecular Microbiology, National Medicines Institute, 00-725 Warsaw, Poland; [email protected] 3 Department of Epidemiology and Clinical Microbiology, National Medicines Institute, 00-725 Warsaw, Poland; [email protected] * Correspondence: [email protected]; Tel.: +48-12-664-6365 Received: 13 October 2020; Accepted: 9 November 2020; Published: 11 November 2020 Abstract: Streptococci and enterococci are significant opportunistic pathogens in epidemiology and infectious medicine. High genetic and taxonomic similarities and several reclassifications within genera are the most challenging in species identification. The aim of this study was to identify Streptococcus and Enterococcus species using genetic and phenotypic methods and to determine the most discriminatory identification method. Thirty strains recovered from clinical samples representing 15 streptococcal species, five enterococcal species, and four nonstreptococcal species were subjected to bacterial identification by the Vitek® 2 system and Sanger-based sequencing methods targeting the 16S rRNA, sodA, tuf, rpoB, and recA genes. Phenotypic methods allowed the identification of 10 streptococcal strains, five enterococcal strains, and four nonstreptococcal strains (Leuconostoc, Granulicatella, and Globicatella genera). The combination of sequencing methods allowed the identification of 21 streptococcal strains, five enterococcal strains, and four nonstreptococcal strains. -
Insights Into 6S RNA in Lactic Acid Bacteria (LAB) Pablo Gabriel Cataldo1,Paulklemm2, Marietta Thüring2, Lucila Saavedra1, Elvira Maria Hebert1, Roland K
Cataldo et al. BMC Genomic Data (2021) 22:29 BMC Genomic Data https://doi.org/10.1186/s12863-021-00983-2 RESEARCH ARTICLE Open Access Insights into 6S RNA in lactic acid bacteria (LAB) Pablo Gabriel Cataldo1,PaulKlemm2, Marietta Thüring2, Lucila Saavedra1, Elvira Maria Hebert1, Roland K. Hartmann2 and Marcus Lechner2,3* Abstract Background: 6S RNA is a regulator of cellular transcription that tunes the metabolism of cells. This small non-coding RNA is found in nearly all bacteria and among the most abundant transcripts. Lactic acid bacteria (LAB) constitute a group of microorganisms with strong biotechnological relevance, often exploited as starter cultures for industrial products through fermentation. Some strains are used as probiotics while others represent potential pathogens. Occasional reports of 6S RNA within this group already indicate striking metabolic implications. A conceivable idea is that LAB with 6S RNA defects may metabolize nutrients faster, as inferred from studies of Echerichia coli.Thismay accelerate fermentation processes with the potential to reduce production costs. Similarly, elevated levels of secondary metabolites might be produced. Evidence for this possibility comes from preliminary findings regarding the production of surfactin in Bacillus subtilis, which has functions similar to those of bacteriocins. The prerequisite for its potential biotechnological utility is a general characterization of 6S RNA in LAB. Results: We provide a genomic annotation of 6S RNA throughout the Lactobacillales order. It laid the foundation for a bioinformatic characterization of common 6S RNA features. This covers secondary structures, synteny, phylogeny, and product RNA start sites. The canonical 6S RNA structure is formed by a central bulge flanked by helical arms and a template site for product RNA synthesis. -
Abiotrophia Defectiva
& Experim l e ca n i t in a l l C C f a Journal of Clinical & Experimental Bajaj et al., J Clin Exp Cardiolog 2013, 4:11 o r d l i a DOI: 10.4172/2155-9880.1000276 o n l o r g u y o J Cardiology ISSN: 2155-9880 Case Report Open Access Aortic Valve Endocarditis by a Rare Organism: Abiotrophia defectiva Anurag Bajaj1*, Parul Rathor2, Ankur Sethi3, Vishal Sehgal4 and Julio A Ramos4 1Wright Center for Graduate Medical Education, Internal Medicine, USA 2Medical College of Zhengzhou University, China 3Rosalind Franklin University, USA 4The Common Wealth Medical College, USA Abstract Abiotrophia defectiva or nutritionally variant Streptococcus (NVS) is rare but important cause of infective endocarditis. We present a case of a 40 year old man with history of aortic valve replacement 14 years ago admitted for fever and chills. Blood culture grew A. defectiva in 4 out of 4 bottles. Patient became a febrile within few days after staring Ceftriaxone but subsequently had renal infarct due to septic embolization. Echocardiogram showed vegetations on aortic valve but no significant aortic regurgitation. After an 8 weeks course of Penicillin and Gentamicin was completed the patient had severe aortic regurgitation. Finally, aortic valve replacement and aortic root replacement was performed and patient did well after the surgery. Clinicians should be aware of this fastidious and aggressive organism when dealing with infective endocarditis. Complications rates are very high even on antibiotics and surgical treatment is needed in at least 50% of the cases. Keywords: Abiotrophia defectiva; Echocardiogram; Embolization; repeat transesophageal echocardiogram showed 4-6 mm vegetation Blood culture on aortic cusp consistent with infective endocarditis without aortic regurgitation (Figure 2).