ACTR2 Rabbit

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Leader in Biomolecular Solutions for Life Science Arp2 Rabbit pAb Catalog No.: A5734 Basic Information Background Catalog No. The specific function of this gene has not yet been determined; however, the protein it A5734 encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through Observed MW lamellipodial actin assembly and protrusion. Two transcript variants encoding different 45kDa isoforms have been found for this gene. Calculated MW 44kDa/45kDa Category Primary antibody Applications WB, IHC, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 10097 P61160 IHC 1:50 - 1:200 Immunogen 1:50 - 1:200 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human Arp2 (NP_005713.1). Synonyms ACTR2;ARP2 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using Arp2 antibody (A5734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded rat brain using Arp2 antibody embedded human uterine cancer using embedded mouse brain using Arp2 (A5734) at dilution of 1:100 (40x lens). Arp2 antibody (A5734) at dilution of 1:100 antibody (A5734) at dilution of 1:100 (40x (40x lens). lens). Immunofluorescence analysis of U2OS cells using Arp2 antibody (A5734). Blue: DAPI for nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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ACTR2 Antibody / Arp2 (RQ5865)
ACTR2 Antibody / Arp2 (RQ5865) Catalog No. Formulation Size RQ5865 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug Bulk quote request Availability 1-3 business days Species Reactivity Human, Mouse, Rat, Monkey Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit IgG Purity Affinity purified Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide UniProt P61160 Applications Western blot : 0.5-1ug/ml Immunohistochemistry : 1-2ug/ml Immunofluorescence : 2-4ug/ml Flow cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml Limitations This ACTR2 antibody is available for research use only. IHC staining of FFPE human breast cancer with ACTR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing. Immunofluorescent staining of FFPE human A549 cells with ACTR2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min. Western blot testing of 1) rat kidney, 2) rat spleen, 3) mouse HEPA1-6, 4) mouse SP2/0, 5) monkey COS-7 and human 6) U-87 MG, 7) Jurkat, 8) PC-3 and 9) U-2 OS lysate with ACTR2 antibody. Predicted molecular weight ~45 kDa. Flow cytometry testing of human A431 cells with ACTR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACTR2 antibody. Flow cytometry testing of mouse ANA-1 cells with ACTR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACTR2 antibody. Description The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex.
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CRAVAT 4: Cancer-Related Analysis of Variants Toolkit David L
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SUPPLEMENTARY APPENDIX Inflammation Regulates Long Non-Coding RNA-PTTG1-1:1 in Myeloid Leukemia
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ACTR2 Antibody
Product Datasheet ACTR2 Antibody Catalog No: #33001 Orders: [email protected] Description Support: [email protected] Product Name ACTR2 Antibody Host Species Rabbit Clonality Polyclonal Purification Antibodies were purified by affinity purification using immunogen. Applications WB,IHC,IF Species Reactivity Human,Mouse,Rat Specificity The antibody detects endogenous level of total ACTR2 protein. Immunogen Type Recombinant Protein Immunogen Description Recombinant protein of human ACTR2. Target Name ACTR2 Other Names ARP2; Accession No. Swiss-Prot:P61160NCBI Gene ID:10097 SDS-PAGE MW 44KD Concentration 1.0mg/ml Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage Store at -20°C Application Details WB 1:500 - 1:2000IHC 1:50 - 1:200IF 1:50 - 1:200 Images Immunofluorescence analysis of U2OS cells using ACTR2 . Blue: DAPI for nuclear staining. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Immunohistochemistry of paraffin-embedded rat brain using ACTR2 at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded human uterine cancer using ACTR2 at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded mouse brain using ACTR2 at dilution of 1:100 (40x lens). Western blot analysis of extracts of various cell lines, using ACTR2 at 1:1000 dilution. Background The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion.
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Received: 9 June 2020 Revised: 20 August 2020 Accepted: 1 September 2020 DOI: 10.1111/gbb.12698 ORIGINAL ARTICLE A long non-coding RNA (Lrap) modulates brain gene expression and levels of alcohol consumption in rats Laura M. Saba1 | Paula L. Hoffman1,2 | Gregg E. Homanics3 | Spencer Mahaffey1 | Swapna Vidhur Daulatabad4 | Sarath Chandra Janga4,5,6 | Boris Tabakoff1 1Department of Pharmaceutical Sciences, Skaggs School of Pharmacy & Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA 2Department of Pharmacology, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA 3Departments of Anesthesiology, Neurobiology and Pharmacology & Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA 4Department of BioHealth Informatics, Indiana University School of Informatics and Computing, Indiana University–Purdue University Indianapolis, Indianapolis, Indiana, USA 5Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, Indiana, USA 6Center for Computational Biology and Bioinformatics, Indiana University School of Medicine, Indianapolis, Indiana, USA Correspondence Boris Tabakoff, Department of Pharmaceutical Abstract Sciences, Skaggs School of Pharmacy & LncRNAs are important regulators of quantitative and qualitative features of the Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, 12850 transcriptome. We have used QTL and other statistical analyses to identify a gene E. Montview Blvd., Aurora, CO 80045. coexpression module associated with alcohol consumption. The “hub gene” of this Email: [email protected] module, Lrap (Long non-coding RNA for alcohol preference), was an unannotated Funding information transcript resembling a lncRNA. We used partial correlation analyses to establish that Banbury Fund; Ethanol Mechanisms in GABAAR gene targeted mice, Grant/Award Lrap is a major contributor to the integrity of the coexpression module.
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Title: a Yeast Phenomic Model for the Influence of Warburg Metabolism on Genetic
bioRxiv preprint doi: https://doi.org/10.1101/517490; this version posted January 15, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 Title Page: 2 3 Title: A yeast phenomic model for the influence of Warburg metabolism on genetic 4 buffering of doxorubicin 5 6 Authors: Sean M. Santos1 and John L. Hartman IV1 7 1. University of Alabama at Birmingham, Department of Genetics, Birmingham, AL 8 Email: [email protected], [email protected] 9 Corresponding author: [email protected] 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 1 bioRxiv preprint doi: https://doi.org/10.1101/517490; this version posted January 15, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 26 Abstract: 27 Background: 28 Saccharomyces cerevisiae represses respiration in the presence of adequate glucose, 29 mimicking the Warburg effect, termed aerobic glycolysis. We conducted yeast phenomic 30 experiments to characterize differential doxorubicin-gene interaction, in the context of 31 respiration vs. glycolysis. The resulting systems level biology about doxorubicin 32 cytotoxicity, including the influence of the Warburg effect, was integrated with cancer 33 pharmacogenomics data to identify potentially causal correlations between differential 34 gene expression and anti-cancer efficacy.
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bioRxiv preprint doi: https://doi.org/10.1101/080432; this version posted February 12, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. A sensitized mutagenesis screen in Factor V Leiden mice identifies novel thrombosis suppressor loci Randal J. Westricka,b,c,h, Kärt Tombergc,e,h, Amy E. Sieberta, Guojing Zhuc, Mary E. Winnd, Sarah L. Dobiesc, Sara L. Manningc, Marisa A. Brakea, Audrey C. Cleurenc, Linzi M. Hobbsa, Lena M. Mishacka, Alexander Johnstona, Emilee N. Kotnikc, David R. Siemieniakf, Jishu Xue, Jun Z. Lie, Thomas L. Saunderse and David Ginsburgc,e,f,h aOakland University Department of Biological Sciences bOakland University Center for Data Science and Big Data Analysis cLife Sciences Institute, University of Michigan dBioinformatics and Biostatistics Core, Van Andel Research Institute eDepartment of Human Genetics, University of Michigan fHoward Hughes Medical Institute, University of Michigan gTransgenic Animal Model Core, University of Michigan hDepartments of Internal Medicine and Pediatrics, University of Michigan iThese authors contributed equally to this work Co-corresponding authors: David Ginsburg, MD. 5214 LSI Building, 210 Washtenaw Avenue, Ann Arbor MI 48109. E- mail: [email protected], Telephone: 734-647-4808, Fax: 734-936-2888. Randal Westrick, PhD. 118 Library Drive, 305 Dodge Hall, Rochester MI, 48309-4479 E-mail: [email protected], Telephone: 248-370-3577. 1 bioRxiv preprint doi: https://doi.org/10.1101/080432; this version posted February 12, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder.
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Effect of Myoferlin Depletion on Breast Cancer Cell Motility THESIS
Effect of Myoferlin Depletion on Breast Cancer Cell Motility THESIS Presented in Partial Fulfillment of the Requirements for the Degree Master of Science in the Graduate School of The Ohio State University By Leonithas Ioannis Volakis Graduate Program in Biomedical Engineering The Ohio State University 2011 Master's Examination Committee: Professor Douglas A. Kniss, Advisor Professor Samir N. Ghadiali Professor Heather M. Powell Copyright by Leonithas Ioannis Volakis 2011 Abstract Cancer metastasis requires tumor cell invasion, migration, and proliferation. Cell migration involves a complex series of processes that extend membrane protrusions to form substrate adhesions that cause cytoskeleton contraction within the cell. Further, cell migration is central to tissue repair, regeneration, development, cancer, and inflammation. The ferlins, an evolutionary conserved protein family, have been implicated as critical to maintaining plasma membranes. Specifically, myoferlin (MYOF), a mammalian ferlin, has been shown as responsible for membrane exocytosis/endocytosis and myoblast fusion. More recent studies have demonstrated that MYOF affects the anti-angiogenic response of endothelial cells and impacts the invasive ability of MDA-MB-231 breast cancer cells. It has also been noted that ablating MYOF using RNAi strategies results in consistent cell proliferation rates and a mesenchymal to epithelial phenotypic transformation. In this study, we present quantified 2D morphologic and migration differences in MDA-MB-231 wild-type (231WT) and RNAi-mediated MYOF-deficient (231MYOFKD) human breast adenocarcinoma (MDA-MB-231) cells. Morphometrics found that MYOF deficiency led to significant differences in lamellipodia number and surface area, filopodia length, and cell surface area. These marked changes could suggest that MYOF plays a role in regulating cytoskeletal arrangement in breast cancer cells.
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