ACTR2 Antibody

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Product Datasheet ACTR2 Antibody Catalog No: #33001 Orders: [email protected] Description Support: [email protected] Product Name ACTR2 Antibody Host Species Rabbit Clonality Polyclonal Purification Antibodies were purified by affinity purification using immunogen. Applications WB,IHC,IF Species Reactivity Human,Mouse,Rat Specificity The antibody detects endogenous level of total ACTR2 protein. Immunogen Type Recombinant Protein Immunogen Description Recombinant protein of human ACTR2. Target Name ACTR2 Other Names ARP2; Accession No. Swiss-Prot:P61160NCBI Gene ID:10097 SDS-PAGE MW 44KD Concentration 1.0mg/ml Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage Store at -20°C Application Details WB 1:500 - 1:2000IHC 1:50 - 1:200IF 1:50 - 1:200 Images Immunofluorescence analysis of U2OS cells using ACTR2 . Blue: DAPI for nuclear staining. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 Immunohistochemistry of paraffin-embedded rat brain using ACTR2 at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded human uterine cancer using ACTR2 at dilution of 1:100 (40x lens). Immunohistochemistry of paraffin-embedded mouse brain using ACTR2 at dilution of 1:100 (40x lens). Western blot analysis of extracts of various cell lines, using ACTR2 at 1:1000 dilution. Background The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2 Note: This product is for in vitro research use only and is not intended for use in humans or animals. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 3.

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ACTR2 Antibody / Arp2 (RQ5865)
ACTR2 Antibody / Arp2 (RQ5865) Catalog No. Formulation Size RQ5865 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug Bulk quote request Availability 1-3 business days Species Reactivity Human, Mouse, Rat, Monkey Format Antigen affinity purified Clonality Polyclonal (rabbit origin) Isotype Rabbit IgG Purity Affinity purified Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide UniProt P61160 Applications Western blot : 0.5-1ug/ml Immunohistochemistry : 1-2ug/ml Immunofluorescence : 2-4ug/ml Flow cytometry : 1-3ug/million cells Direct ELISA : 0.1-0.5ug/ml Limitations This ACTR2 antibody is available for research use only. IHC staining of FFPE human breast cancer with ACTR2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing. Immunofluorescent staining of FFPE human A549 cells with ACTR2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min. Western blot testing of 1) rat kidney, 2) rat spleen, 3) mouse HEPA1-6, 4) mouse SP2/0, 5) monkey COS-7 and human 6) U-87 MG, 7) Jurkat, 8) PC-3 and 9) U-2 OS lysate with ACTR2 antibody. Predicted molecular weight ~45 kDa. Flow cytometry testing of human A431 cells with ACTR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACTR2 antibody. Flow cytometry testing of mouse ANA-1 cells with ACTR2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ACTR2 antibody. Description The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex.
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bioRxiv preprint doi: https://doi.org/10.1101/517490; this version posted January 15, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 1 Title Page: 2 3 Title: A yeast phenomic model for the influence of Warburg metabolism on genetic 4 buffering of doxorubicin 5 6 Authors: Sean M. Santos1 and John L. Hartman IV1 7 1. University of Alabama at Birmingham, Department of Genetics, Birmingham, AL 8 Email: [email protected], [email protected] 9 Corresponding author: [email protected] 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 1 bioRxiv preprint doi: https://doi.org/10.1101/517490; this version posted January 15, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. 26 Abstract: 27 Background: 28 Saccharomyces cerevisiae represses respiration in the presence of adequate glucose, 29 mimicking the Warburg effect, termed aerobic glycolysis. We conducted yeast phenomic 30 experiments to characterize differential doxorubicin-gene interaction, in the context of 31 respiration vs. glycolysis. The resulting systems level biology about doxorubicin 32 cytotoxicity, including the influence of the Warburg effect, was integrated with cancer 33 pharmacogenomics data to identify potentially causal correlations between differential 34 gene expression and anti-cancer efficacy.
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bioRxiv preprint doi: https://doi.org/10.1101/080432; this version posted February 12, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. A sensitized mutagenesis screen in Factor V Leiden mice identifies novel thrombosis suppressor loci Randal J. Westricka,b,c,h, Kärt Tombergc,e,h, Amy E. Sieberta, Guojing Zhuc, Mary E. Winnd, Sarah L. Dobiesc, Sara L. Manningc, Marisa A. Brakea, Audrey C. Cleurenc, Linzi M. Hobbsa, Lena M. Mishacka, Alexander Johnstona, Emilee N. Kotnikc, David R. Siemieniakf, Jishu Xue, Jun Z. Lie, Thomas L. Saunderse and David Ginsburgc,e,f,h aOakland University Department of Biological Sciences bOakland University Center for Data Science and Big Data Analysis cLife Sciences Institute, University of Michigan dBioinformatics and Biostatistics Core, Van Andel Research Institute eDepartment of Human Genetics, University of Michigan fHoward Hughes Medical Institute, University of Michigan gTransgenic Animal Model Core, University of Michigan hDepartments of Internal Medicine and Pediatrics, University of Michigan iThese authors contributed equally to this work Co-corresponding authors: David Ginsburg, MD. 5214 LSI Building, 210 Washtenaw Avenue, Ann Arbor MI 48109. E- mail: [email protected], Telephone: 734-647-4808, Fax: 734-936-2888. Randal Westrick, PhD. 118 Library Drive, 305 Dodge Hall, Rochester MI, 48309-4479 E-mail: [email protected], Telephone: 248-370-3577. 1 bioRxiv preprint doi: https://doi.org/10.1101/080432; this version posted February 12, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder.
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