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The Population Status of Lagochilus Setulosus Vved. and Its Biochemical Composition

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The Population Status of Lagochilus Setulosus Vved. and Its Biochemical Composition Ecological Questions 32 (2021) 2: 111-118 http://dx.doi.org/10.12775/EQ.2021.018 The population status of Lagochilus setulosus Vved. and its biochemical composition Arystanbek Eshibaev1, Zhanar Aimenova2,*, Lyazzat Akynova3, Lyazzat Nurseitova3, Aizhan Kopabaeva3 1ShymkentUniversity, Shymkent, 160031, Zhibekzholy street 131, Kazakhstan 2Muhtar Auezov South Kazakhstan University, Shymkent, 160012, Tauke Khan avenue 5, Kazakhstan 3South KazakhstanState Pedagogical University,Shymkent, 160012, Baitursinov street 13, Kazakhstan *corresponding author e-mail: [email protected] Received: 30 January 2021 / Accepted: 15 March 2021 Abstract. This article describes endemic species of the genus Lagochilus growing in the territory of South Kazakhstan – Lagochilus setulosus Vved. Its biochemical composition, namely qualitative and quantitative composition of biologically active compounds was investigated. The established composition of substances indicates that this plant species can be used in pharmaceutical biotechnology to create a biological preparation with a hemostatic effect based on Lagochilus setulosus. Keywords: Lagochilus setulosus, South Kazakhstan, leaves and flowers, hemostatic agent, biological active compounds, deterpenes, lagochilin, lagochirzin. 1. Introduction tive compounds that will provide a long-term reliable raw material base for the pharmaceutical and cosmetology in- Wild flora of South Kazakhstan is represented by more dustries of Kazakhstan. Identification of the content of the than 3000 species of vascular plants. According to scien- main groups of important biologically active compounds tists, 25% of them are medicinal plants. Many species are in growing plant species will allow rational use of plant of practical value for the purposes of traditional and al- resources and will make it possible to significantly expand ternative medicine. However, the medicinal properties of the range of herbal medicinal raw materials. Some of these many of them still remain unexplored. plants are plants of the genus Lagochilus. There are more than 3000 plant species in the flora of Plants of the genus Lagochilus belong to the Lamiaceae South Kazakhstan, of which 553 are of interest as valu- family and are represented by 35 species distributed mainly able natural sources of medicinal substances (Flora of Ka- in Central Asia: in Kazakhstan, Iran, Afghanistan, Russia, zahstan, 1964). However, most of these plant species are Mongolia and China (Rehinger & Hedzh, 1982). Some still insufficiently known. Only a limited number of plant plants of this genus are used as hemostatic agents, for the species are used in the domestic pharmaceutical and cos- treatment of allergic dermatoses (Panossian & Wikman, metic industries. In this regard, the priority direction of 1982), and are also used as a medicine for clot retraction research is to identify potential sources of biologically ac- (Akopov, 1954) and glaucoma (Kadyroza, 1955). Among 112 Arystanbek Eshibaev, Zhanar Aimenova, Lyazzat Akynova, Lyazzat Nurseitova, Aizhan Kopabaeva the various plant species of the genus Lagochilus, Lagochi- 2) The presence of tannins was determined by a specif- lus inebrians Bunge has long been used in folk medicine of ic gelatin deposition reaction (Sumina et al., 2006). the East as a medicinal plant due to its sedative properties 3) The presence of essential oil was determined by the (Perry et al., 2002). method described in State Pharmacopeia X (State However, according to Akopov I.E., Lagochilus setu- Pharmacopoeia of USSR, 1968). losus has the most pronounced stimulating effect on the 4) The presence of cardiac glycosides was determined blood clotting process (Akopov, 1981). However, this type by Keller-Kiliani Reaction (Syvkinet al., 1999). of plant is still insufficiently known and requires further 5) The presence of saponins was determined by the biological and chemical research. reaction to foaming (Ladygina et al., 1983). To solve this problem, we have already performed 6) The presence of phenolglycosides was determined studies on the macro-and micromorphological features of as follows (Petrov, 1978). Lagochilus setulosus (Aimenova et al., 2015a).As a result 7) The presence of iridoids was determined by the of the research, the anatomical structure of this plant was method described in State Pharmacopeia XI (State established using the method of scanning electron micros- Pharmacopoeia of USSR, 1989). copy. Gandular trichomes were found. 8) The presence of flavonoids was determined by cya- Also was performed a comparative research of the mac- nidin sample (Bandyukova, 1965). ro – and microelement composition of Lagochilus inebri- 9) The presence of coumarins was determined as fol- ans and Lagochilus setulosus (Aimenova et al., 2014). As lows: for the preparation of extraction from veg- a result of research, it was found that these plants differ in etable raw materials 2 g of crushed raw materials the quantitative content of macro-and microelements. Ac- (crushed flowers and leaves of plants), 20 ml of cording to the content of trace elements, the plant Lagochi- ethyl alcohol was poured and boiled for 15 min- lus inebrians surpasses Lagochilus setulosus, but the quan- utes with a reflux. After cooling, filtered. To 3-5 titative content of iron, magnesium, lithium, and aluminum ml of alcoholic extract was added 10 drops of 10% is 2-3 times higher in Lagochilus setulosus. At the same KOH in methanol and heated for 5 min in a water time, of all the identified macro – and microelements in bath (solution turned yellow), then added 5 drops of Lagochilus setulosus, as well as in Lagochilus inebrians, freshly prepared diazo reagent of Pauli Kutochku. the percentage content contains the most calcium, which Further, the solution acquired a cherry color, which in the presence of lagochirzin fully implements the process indicates the presence of coumarins. of plasma hemostasis. 10) The presence of anthracene derivatives was de- The next, necessary stage of the study of this plant spe- termined by the following method (Kurkin et al., cies is the study of its distribution area, as well as the study 2016). of its biological composition. 11) The presence of sugars was determined by the meth- od (Fialkov, 1946). 12) The presence of alkaloids was determined by the 2. Objects and methods method (Ladygina, 1983). 13) The presence of resinous substances was deter- For the research, the flowers and leaves of Lagochilus setu- mined by the decrease in the mass of the sample losus were collected during the mass flowering period in after exhaustive extraction in the soxlet apparatus July. To do this, the tops of the stems of plants 20-30 cm with a capacity of 1 l for 20 h and subsequent dry- long were cut off, leaving 1-2 plants per 1m2 for seed- ing to a constant weight at a temperature of 105°C. ing and population renewal. Drying was carried out in the Further, the method of fractionation using chloro- shade in a well-ventilated room, laying it out in a thin layer form and acetone solvents was used to separate res- on a tarpaulin. Then, after drying, the stems were threshed inous substances (Kuznetsov et al., 2004). with a wooden stick, separated and discarded. Then the Quantitative determination of the content of the main yellowed and browned parts were removed and the raw groups of biologically active substances of Lagochilus materials were crushed in a mechanical shredder. Thus, setulosus performed according to the following methods: the finished raw material contained flowers and leaves that 1) essential oil by the method of (State Pharmacopoeia of retained their natural color. the USSR, 1968). Qualitative determination of the content of the main 2) resinous substances by the method: the leaves and flow- groups of biologically active substances in the phytomass ers of Lagochilus setulosus crushed to a particle size of of Lagochilus setulosus was performed according to the 1-2 mm and subjected to extraction of non-polar sol- following methods: vent, hexanal to conventional Soxhlet extractions for 1) The presence of diterpenes was determined by paper the separation of resinous substances. The yield of res- chromatography (Belenkij et al., 1983). inous substances depending on the terms of raw mate- V2 – the volume of ethanol extract was taken for use on chromatogram, ml; V2 – the volume of ethanol extract was taken for use on chromatogram, ml; V3 – the volume of eluate; The population status of Lagochilus setulosus Vved.V2 – and the itsvolume biochemical of ethanol composition extract was taken for use 113on chromatogram, ml; V3 – the volume of eluate; D363 – optical density of solution at λ = 363 nm; V3 – the volume of eluate; D – optical density of solution at λ = 363 nm; D1%/1cm363 – the rate of absorption of rutinat λ = 363 nm (268.4); D363 – optical density of solution at λ = 363 nm; rial preparation and storage was 6-9% of the weight of D1%/1cmD1%/1cm – the – therate rate of ofabsorption absorption of of rutinat rutinat λ λ= = 363 363 nmnm (268.4); m – Dthe1%/1cm weight – of the the rate portion of absorption of the raw of material, rutinat λ g=; 363 nm (268.4); the absolutely dry residue. m – the weight(268.4); of the portion of the raw material, g; 3) diterpene1) essentiallagochilin oil according by the method to the of method: (State Pharmacopoeia 10 g of mw – of mlossthe the – theweightin USSR weightweight ,of 1968 of theof raw )theportion. portionmaterials of theof during the raw raw material, drying, material, %; g; g ; finely powdered raw material
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