KCTD13 PROX1
IRF3
KLRG1
TJP1
COL12A1
LMBRD2
SLC25A32
TRO
RASD1
HECW2 CXCR4 IRF2BPL
PTPDC1
CD55 PSAT1 A MOV10L1SPRED2 TSGA10
BAIAP2 SLC1A4 TMEM39A
PPAP2B
BCL3 ARL4A
RAPGEF1
VEGFC F3 PHLDB2 UNC5B PRDM1
LEPREL1 NR4A2 NR4A1 RAPH1
SOCS6 SULT1B1 TNFAIP3
RGS2
GIMAP1 KIAA0513 STEAP2 HSP90B1 MAP3K8 SLC25A25 TNFRSF1B DDIT4 PLEKHA1 FRMD6 C2CD4A
TSPAN11
IGIP
UACA RFX2 PMAIP1
IL15RA
SLC2A12 MOSPD1 GIMAP6 EDN1 ADAM19 NCOA7
CLDN5 CHSY3 BCAP29
PDP1 UBE2J1 RBPMS2 PPFIBP2 KAT2B PLAU SIPA1L3 FOXN3 C14orf93 KIF26A EGR1 ACER2 MEI1 GAB1 ASUN
CNN3 SIRT1
RALGPS2 PPM1H TOX2 FAM174A DNAJB4 KLF11
GBE1 CHN2 TRIM2 IL1R1 KCNJ2 TFPI2 KLF2 PPP1R3B PPARA PPP1R15B VEGFA DSE CCRN4L NR2F2 AXL EXOC8 CCNA1 B4GALT1 GIMAP7 RQCD1 ITPRIP MYADM ICAM3
FGF2 HEY1 NFIL3
CTH PTP4A3 ATP2B1 EXT1 KLHL3 KLF9 COL13A1
MID2 GDPD1
EHD3 STRBP P4HA1 OSBP2
SEC11C NFAT5 PDGFD
ENC1 DUSP5 NRCAM ANO6 KLF10 SLC40A1 C2CD4B MEIS1HOXB2_ELF1 GIMAP2 SEMA6A NR4A3 KDM6B
MYO1D FAM193A
CSRNP2 RASGRF2 APOO ESYT2 NINL FOXP1 HDAC9 CLK1 CDK6 SCAMP5 PHLDB3 ASGR1
HELLS DIEXF SULT1C4 MBNL1 TRAF5 GIMAP8 FOXJ2_ELF1 SIX4 TEAD4_ERG ARL4C
EGR3 BACH1 ITGA6 LRRC8B MFSD2A SLC4A7
RDX
PLAT PODXL USF2 NR5A2 RALGAPA2 DDX60L ANGPT2 CSF1 FOXO1_ELF1 ATP1B1 CTCF P4HA3 DNAJC3
CREG1 ACRBP BRAT1 RHOBTB1 TEAD2 ARID5A
FBXL2 SLC12A4 SPRY4 CDC14A ATF3 ETV5_HOXA2 CHST11 GCNT1 ESM1 FZD8
IVNS1ABP MGP CROCC
SLC39A14 DUSP7 MAP2K6 TOB2 SLC35G2 SPTY2D1 BMP4 ERG ST8SIA4 POLI MCL1 SOX13 CROT
CGNL1 MXD1 ETS1 THBD BACH2 ELF1 PLAUR STC1 SGK3 ITPR2 IRAK2 PHC3 FAM198B AIM1
RNF122
ETV3
FKBP14 PIM1 MX1 TNIK MMP10
ST3GAL5 DUSP1 GULP1 INPP5A PSTPIP2 MEF2C PNP IPMK STOML1 ARHGEF18 ICOSLG MYO1E ZBTB18 PMCH MBNL3 RIOK2 TNFSF10 KITLG DHRS1 C1orf198 TMEM2 KRT80 NID2 SERP1 WNT9A PLCXD1 HBEGF PDE2A
EDEM1 STEAP1 FLT1 SDPR NEDD4L SEMA6D CLASP2
ARHGAP28 SSBP3 FBXO32 ELL2 EDIL3 ACKR3 SYNM
TFPI
XPA DOK5 HTR1B EDEM3 ZNF217 DDI2 CPEB4 SSH1 SEL1L COLQ ZNF267 RCAN1 NAB2
PDGFA
GPX8 ZBTB10 DLL4 LHX6 TSPAN13 FKBP11 PLXNA2 B4GALT5 CARS NEDD9 TMCO4 PTGER4
NANS
MYRIP CTSH
KRT19 SEPT5 DLG1
RCAN3
RNF144A MEGF6
VGLL4 CREM RFC3 HSD17B2 NFE2L2 MAFF
CD200 PLVAP PDIA4 C12orf44 ZCCHC14 CHST1
MYBL2 SLC19A2
TRIB3 RASGRP3
YRDC
RP9
SERINC5 GPR176 RNF145
AHNAK2
MCM10 KCTD12 IDH3A NR1H3 ETV5 ID3
ADAMTS9 PELO WASF3 BCL6MIDN
PLLP SETD8
ATP8B1
EFNA5
ATP8B2
NT5E
VIMP SLC41A2
HSPA5 M3: M1: PEX5 M2: Response to hydrogen peroxide response to endoplasmic reticulum stress Regulation of cellular extravasation Positive regulation of endothelial Cellular polysaccharide biosynthetic process PDGF receptor signaling pathway cell proliferation
CCNG2
FAT1
TOX
MYO15B
ATP5H
RBPJ
CABLES2
NCAPG
TCF15
POLE4
RGCC
DHRS3
PFKFB3 ZNF143 PCIF1
ARSJ ZNF697 TBCC
FLRT2 N4BP2
ZBTB21
DPF3 NAMPTL NUP85 ZNF76 COL4A3BP PTGS2 TRIB1 GSDMD NRG1
PRKACB TM4SF18 CNKSR3
APOLD1 ITGAV LYSMD3
IL6ST ANGPTL2 IL1A TRAF1
BHLHE40 NR2F1 NDRG1 RIPK2 SLC7A5 C10orf128
SLC38A2
SERPINH1 C8orf4 B3GNT5 CSRNP1
GADD45A
TPP2
MFSD6
RAPGEF5 PALLD USP53 CREB5 CXCL2 SLC26A4 MDM2 C10orf10 B4GALT4 COL8A1
CXCL1 ZEB2 POLR3D IL8
NYNRIN IFRD1
SIK1 PDE3A SLC10A7 GABPA PER1 BIRC3 NFKB2 SMAD3 ZNF704
TIPARP
TLE4 PDE4D SAT1 GARS RPS6KA1
GPR126 FOXO1_ELK1 ATP2A2
MN1 NOS3
GBP1
RUNX1 FOXO1_ETV1 PITPNC1 NUCB2 MEX3C
PKIG BMX ITGB3 FOSL1 MYL4 DKK1 ZC3H12A TSHZ1 HMGN4 IRF2 TNFSF15
AQP1
CRELD2 NF1GATA3 ANK3
CCL2
TSC22D4 BBC3 LIPG SOX4 ETV5_FOXO1ERF_FOXO1 RARG HDAC5 HK2 SLC7A1 HIVEP2 JUN TMEM173 JUN-AP1 FOSL2 ABCC4
CLDN11 DIP2C NOG MOSPD2 GCH1 CD40 TSC22D2
FOSB LARP1B
PIK3R1 GPRC5A SETX ATF1
EIF2B3 PALD1
FOXO1_ETV4 FARS2 HSPA13
TBX18 KCNN3
KLF4 HHIP GNG2
IL1RL1 PARP12 FAM124B ELK4 XBP1 PTX3 THSD1 SERTAD3
STC2 IDI1 ALDH1A1 RAB3B SERPINE1
TRIB2 VCAM1 SELE
ITGB8
SLC9A3R2 FOXO1CHST3 GDF6 CCL20
MTHFD2
RND3 MEF2A SLC12A2
GCOM1
A2M STARD4 GATA6 CAMK2N1 IL33 RNF144B HERPUD1
DTNB
SDC4 YPEL2 SOD2 IL6
FOXF1 ZC3H12C LAMP3 ADTRP DCBLD2
SLC30A7
ZFAND2A PJA2
TSPAN5 SLC33A1
IRS2
SGK1 UGDH NRG3
CXCL3
FAM185A
MIS12
SLC30A1
RELB PCDH12 ALAD
PPIL4
SEMA6C IL27RA
CIC
ICAM1
PPP1R15A M4: M5： ADAMTS1 M6: Positive regulation of cytokine secretion Nitric oxide metabolic process Muscle cell proliferation; Negative regulation of endothelial cell migration Regulation of muscle adaptation Positive regulation of leukocyte migration
MAFG B C 20 D 15 VEGF 10
HPAEC HUVEC ECFC HMVEC FPKM 5 0H 1H 4H 12H MAFF 0 MAFF H0 H1 H4 H12 MAFG MAFG MAFK 10 MAFK 8 MAFK 6 Tubulin Tubulin 4 FPKM 2 0 H0 H1 H4 H12
150 MAFF
100
FPKM 50
0 H0 H1 H4 H12
Supplemental Fig. S7. MAFs are master regulators of VEGF transcriptional network. (A) Sub-networks from Fig. 7B. The VEGFA gene regulatory network contained six major sub-modules showing diverse functions. (B). Western-Blot detecting MAFs expression in four different types of ECs. HPAEC: human pulmonary artery endothelial cell; HUVEC: human umbilical vein endothelial cell; ECFC: endothelial clone forming cell, HMVEC: human dermal microvascular Endothelial cell. (C) VEGFA upregulated expression of MAF factors at H1, as revealed by RNA-seq. (D) VEGFA upregulat- ed the expression of MAF proteins at H1, as revealed by western-blot.