KCTD13 PROX1 IRF3 KLRG1 TJP1 COL12A1 LMBRD2 SLC25A32 TRO RASD1 HECW2 CXCR4 IRF2BPL PTPDC1 CD55 PSAT1 A MOV10L1SPRED2 TSGA10 BAIAP2 SLC1A4 TMEM39A PPAP2B BCL3 ARL4A RAPGEF1 VEGFC F3 PHLDB2 UNC5B PRDM1 LEPREL1 NR4A2 NR4A1 RAPH1 SOCS6 SULT1B1 TNFAIP3 RGS2 GIMAP1 KIAA0513 STEAP2 HSP90B1 MAP3K8 SLC25A25 TNFRSF1B DDIT4 PLEKHA1 FRMD6 C2CD4A TSPAN11 IGIP UACA RFX2 PMAIP1 IL15RA SLC2A12 MOSPD1 GIMAP6 EDN1 ADAM19 NCOA7 CLDN5 CHSY3 BCAP29 PDP1 UBE2J1 RBPMS2 PPFIBP2 KAT2B PLAU SIPA1L3 FOXN3 C14orf93 KIF26A EGR1 ACER2 MEI1 GAB1 ASUN CNN3 SIRT1 RALGPS2 PPM1H TOX2 FAM174A DNAJB4 KLF11 GBE1 CHN2 TRIM2 IL1R1 KCNJ2 TFPI2 KLF2 PPP1R3B PPARA PPP1R15B VEGFA DSE CCRN4L NR2F2 AXL EXOC8 CCNA1 B4GALT1 GIMAP7 RQCD1 ITPRIP MYADM ICAM3 FGF2 HEY1 NFIL3 CTH PTP4A3 ATP2B1 EXT1 KLHL3 KLF9 COL13A1 MID2 GDPD1 EHD3 STRBP P4HA1 OSBP2 SEC11C NFAT5 PDGFD ENC1 DUSP5 NRCAM ANO6 KLF10 SLC40A1 C2CD4B MEIS1HOXB2_ELF1 GIMAP2 SEMA6A NR4A3 KDM6B MYO1D FAM193A CSRNP2 RASGRF2 APOO ESYT2 NINL FOXP1 HDAC9 CLK1 CDK6 SCAMP5 PHLDB3 ASGR1 HELLS DIEXF SULT1C4 MBNL1 TRAF5 GIMAP8 FOXJ2_ELF1 SIX4 TEAD4_ERG ARL4C EGR3 BACH1 ITGA6 LRRC8B MFSD2A SLC4A7 RDX PLAT PODXL USF2 NR5A2 RALGAPA2 DDX60L ANGPT2 CSF1 FOXO1_ELF1 ATP1B1 CTCF P4HA3 DNAJC3 CREG1 ACRBP BRAT1 RHOBTB1 TEAD2 ARID5A FBXL2 SLC12A4 SPRY4 CDC14A ATF3 ETV5_HOXA2 CHST11 GCNT1 ESM1 FZD8 IVNS1ABP MGP CROCC SLC39A14 DUSP7 MAP2K6 TOB2 SLC35G2 SPTY2D1 BMP4 ERG ST8SIA4 POLI MCL1 SOX13 CROT CGNL1 MXD1 ETS1 THBD BACH2 ELF1 PLAUR STC1 SGK3 ITPR2 IRAK2 PHC3 FAM198B AIM1 RNF122 ETV3 FKBP14 PIM1 MX1 TNIK MMP10 ST3GAL5 DUSP1 GULP1 INPP5A PSTPIP2 MEF2C PNP IPMK STOML1 ARHGEF18 ICOSLG MYO1E ZBTB18 PMCH MBNL3 RIOK2 TNFSF10 KITLG DHRS1 C1orf198 TMEM2 KRT80 NID2 SERP1 WNT9A PLCXD1 HBEGF PDE2A EDEM1 STEAP1 FLT1 SDPR NEDD4L SEMA6D CLASP2 ARHGAP28 SSBP3 FBXO32 ELL2 EDIL3 ACKR3 SYNM TFPI XPA DOK5 HTR1B EDEM3 ZNF217 DDI2 CPEB4 SSH1 SEL1L COLQ ZNF267 RCAN1 NAB2 PDGFA GPX8 ZBTB10 DLL4 LHX6 TSPAN13 FKBP11 PLXNA2 B4GALT5 CARS NEDD9 TMCO4 PTGER4 NANS MYRIP CTSH KRT19 SEPT5 DLG1 RCAN3 RNF144A MEGF6 VGLL4 CREM RFC3 HSD17B2 NFE2L2 MAFF CD200 PLVAP PDIA4 C12orf44 ZCCHC14 CHST1 MYBL2 SLC19A2 TRIB3 RASGRP3 YRDC RP9 SERINC5 GPR176 RNF145 AHNAK2 MCM10 KCTD12 IDH3A NR1H3 ETV5 ID3 ADAMTS9 PELO WASF3 BCL6MIDN PLLP SETD8 ATP8B1 EFNA5 ATP8B2 NT5E VIMP SLC41A2 HSPA5 M3: M1: PEX5 M2: Response to hydrogen peroxide response to endoplasmic reticulum stress Regulation of cellular extravasation Positive regulation of endothelial Cellular polysaccharide biosynthetic process PDGF receptor signaling pathway cell proliferation CCNG2 FAT1 TOX MYO15B ATP5H RBPJ CABLES2 NCAPG TCF15 POLE4 RGCC DHRS3 PFKFB3 ZNF143 PCIF1 ARSJ ZNF697 TBCC FLRT2 N4BP2 ZBTB21 DPF3 NAMPTL NUP85 ZNF76 COL4A3BP PTGS2 TRIB1 GSDMD NRG1 PRKACB TM4SF18 CNKSR3 APOLD1 ITGAV LYSMD3 IL6ST ANGPTL2 IL1A TRAF1 BHLHE40 NR2F1 NDRG1 RIPK2 SLC7A5 C10orf128 SLC38A2 SERPINH1 C8orf4 B3GNT5 CSRNP1 GADD45A TPP2 MFSD6 RAPGEF5 PALLD USP53 CREB5 CXCL2 SLC26A4 MDM2 C10orf10 B4GALT4 COL8A1 CXCL1 ZEB2 POLR3D IL8 NYNRIN IFRD1 SIK1 PDE3A SLC10A7 GABPA PER1 BIRC3 NFKB2 SMAD3 ZNF704 TIPARP TLE4 PDE4D SAT1 GARS RPS6KA1 GPR126 FOXO1_ELK1 ATP2A2 MN1 NOS3 GBP1 RUNX1 FOXO1_ETV1 PITPNC1 NUCB2 MEX3C PKIG BMX ITGB3 FOSL1 MYL4 DKK1 ZC3H12A TSHZ1 HMGN4 IRF2 TNFSF15 AQP1 CRELD2 NF1GATA3 ANK3 CCL2 TSC22D4 BBC3 LIPG SOX4 ETV5_FOXO1ERF_FOXO1 RARG HDAC5 HK2 SLC7A1 HIVEP2 JUN TMEM173 JUN-AP1 FOSL2 ABCC4 CLDN11 DIP2C NOG MOSPD2 GCH1 CD40 TSC22D2 FOSB LARP1B PIK3R1 GPRC5A SETX ATF1 EIF2B3 PALD1 FOXO1_ETV4 FARS2 HSPA13 TBX18 KCNN3 KLF4 HHIP GNG2 IL1RL1 PARP12 FAM124B ELK4 XBP1 PTX3 THSD1 SERTAD3 STC2 IDI1 ALDH1A1 RAB3B SERPINE1 TRIB2 VCAM1 SELE ITGB8 SLC9A3R2 FOXO1CHST3 GDF6 CCL20 MTHFD2 RND3 MEF2A SLC12A2 GCOM1 A2M STARD4 GATA6 CAMK2N1 IL33 RNF144B HERPUD1 DTNB SDC4 YPEL2 SOD2 IL6 FOXF1 ZC3H12C LAMP3 ADTRP DCBLD2 SLC30A7 ZFAND2A PJA2 TSPAN5 SLC33A1 IRS2 SGK1 UGDH NRG3 CXCL3 FAM185A MIS12 SLC30A1 RELB PCDH12 ALAD PPIL4 SEMA6C IL27RA CIC ICAM1 PPP1R15A M4: M5： ADAMTS1 M6: Positive regulation of cytokine secretion Nitric oxide metabolic process Muscle cell proliferation; Negative regulation of endothelial cell migration Regulation of muscle adaptation Positive regulation of leukocyte migration MAFG B C 20 D 15 VEGF 10 HPAEC HUVEC ECFC HMVEC FPKM 5 0H 1H 4H 12H MAFF 0 MAFF H0 H1 H4 H12 MAFG MAFG MAFK 10 MAFK 8 MAFK 6 Tubulin Tubulin 4 FPKM 2 0 H0 H1 H4 H12 150 MAFF 100 FPKM 50 0 H0 H1 H4 H12 Supplemental Fig. S7. MAFs are master regulators of VEGF transcriptional network. (A) Sub-networks from Fig. 7B. The VEGFA gene regulatory network contained six major sub-modules showing diverse functions. (B). Western-Blot detecting MAFs expression in four different types of ECs. HPAEC: human pulmonary artery endothelial cell; HUVEC: human umbilical vein endothelial cell; ECFC: endothelial clone forming cell, HMVEC: human dermal microvascular Endothelial cell. (C) VEGFA upregulated expression of MAF factors at H1, as revealed by RNA-seq. (D) VEGFA upregulat- ed the expression of MAF proteins at H1, as revealed by western-blot. .