DOCSLIB.ORG

Setting New Standards Absoluteidq® Stero17 Kit

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Setting New Standards Absoluteidq® Stero17 Kit Setting new standards AbsoluteIDQ® Stero17 Kit Experience the power of multiplexing steroid hormones Steroid hormones: More than simple messengers Steroid hormones are responsible for the regulation of a multitude of physiological functions. Gluco- corticoids (e.g., cortisol) participate in the carbo- hydrate, lipid, and albumin metabolism and play a major role in stress situations. Mineralocorticoids (e.g., aldosterone) primarily influence the water and mineral household. Estrogens (e.g., estradiol, estrone), androgens (e.g., testosterone), and gesta- gens (e.g., progesterone) regulate the reproduction processes and the formation of secondary sexual characteristics. As steroid hormones influence a broad range of pathways within the organism, it is important to detect early changes and dysregulations in the steroid hormone metabolism. Steroid hormone disorders: inborn or acquired Many endocrine diseases are caused by disorders of the human steroid metabolism. These disorders can be: • inborn (manifested in early childhood such as congenital adrenal hyperplasia (CAH)) • caused by diseases (e.g., Cushing syndrome, tumors of the adrenal cortex, hyperaldosteronism) Steroid Hormones 1 Aldosterone Pregnanediol Allopregnanolone Pregnanetriol Boundless opportunities through 2 Androstenedione 3 Androsterone Multiplexing 4 Corticosterone Pregnenolone Progesterone 11-Deoxycorticosterone Corticosterone THCSt 5 Cortisol 6 Cortisone Simultaneous detection of 7 11-Deoxycorticosterone 17-OH Pregnenolone 17-OH Progesterone 11-Deoxycortisol a broad range of steroid 8 11-Deoxycortisol 21-Deoxycortisol hormones (3 classes: Sex 9 Dehydroepiandrosterone (DHEA) 10 Dehydroepiandrosterone-Sulphate (DHEAS) DHEAS DHEA Androstenedione Cortisol steroids, mineralocorticoids, Aldosterone 11 Dihydrotestosteron (DHT)* Cortisone glucocorticoids) 12 Estradiol (E2) Testosterone 13 Estrone (E1) Etiocholanolone Androsterone E1-S 14 Etiocholanolone Estrone (E1) 15 17 -Hydroxyprogesterone 16 Progesteronea 2-OH Estrone Dihydroandrosterone DHT 7-Dehydroestrone 17 Testosterone Estradiol (E2) *semi quantitative Estriol (E3) • caused by therapies (e.g., glucocorticoid treat- ® ment in patients with allergic, rheumatological, or AbsoluteIDQ Stero17 Kit – gastrointestinal diseases) or operative intervention Key Benefi ts • caused by other interferences in the hormone metabolism (e.g., administration of steroid • More High Throughput with UHPLC-MS/MS hor mones in contraception, pre- and post-meno- (96 Tests in 12 hours) pausal conditions, in vitro fertilization, polycystic • Simplyfi ed and Faster Sample Preparation ovary syndrome (PCOS)) • Now DHT included • 3 classes: sex steroids, mineralocorticoids, gluco- Innovation ”Ready-to-use” corticoids • Ready-to-use kit AbsoluteIDQ® Stero17 Kit offers a unique experi- • Multiplexing steroid hormones ence in standardized, quantitative steroid hormone • Most comprehensive steroid hormone screening detection by simultaneously analyzing (multiplex- tool ing) 17 different steroid hormones with (Ultra) High • Flexible sample number batches Performance Liquid Chromatography tandem mass • Developed for HPLC- as well as for improved high spectrometry ((U)HPLC-MS/MS). throughput UHPLC-MS/MS analysis • High quality standards and reliable results through This enables the generation of a comprehensive QC-implemented workfl ow control steroid profi le from one single human blood (serum, • Highly robust kit technology with superior analytical plasma) sample by covering a broad range of poten- performance tial indications. By this means, potential dysregula- • Extraordinary analytical precision, accuracy, selec- tions in the steroid hormone biosynthesis can be de- tivity and sensitivity tected early, while simultanously quantifying a broad range of steroid hormones. With unprecedented accuracy, reproducibility, and pre- ® cision, AbsoluteIDQ Stero17 Kit sets new standards Literature: by assuring high-quality, robust, and reliable results – Koal T, Schmiederer D, Pham-Tuan H, Röhring C, Rauh M. Standard- izded LC-MS/MS based steroid hormone profi le-analysis. J Steroid which is also proven by profi ciency tests. Biochem Molecul Biolog 129 (2012) 129-138. Steroid hormone profi ciency test Hitting the target – Setting new standards AbsoluteIDQ® Stero17 Kit demonstrated excellent * accuracy during a round robin * including seven different steroid hormones Cortisol Estradiol DHEAS 17-OHP Aldosterone ProgesteroneTestosterone *SteroIDQ® Biocrates – The Deep Phenotyping Company BIOCRATES Life Sciences AG offers unique solutions for targeted, quantitative and quality controlled Metabolic Phenotyping (“Deep Pheno- typing”). Our mass spectrometry based solutions deliver unprecedented accuracy, precision and multiplex- ing capabilities for a comprehensive and predic- tive understanding of phenotypes and metabolic pathways. Our metabolomics solutions set a new level for standardized data processing, exchange and storage that can readily meet the rigorous de- mands of various disciplines such as life sciences, personalized medicine, clinical research, and drug development. Ordering Information AbsoluteIDQ® Stero 17 Kit Size AbsoluteIDQ® Stero17 Kit – HPLC Set 96 Tests (flexible batches) AbsoluteIDQ® Stero17 Kit – UHPLC Set 96 Tests (flexible batches) BIOCRATES Life Sciences AG Eduard-Bodem-Gasse 8, 6020 Innsbruck, Austria phone: +43.512.579 823, fax: +43.512.579 823-329 email: [email protected], www.biocrates.com.
Load more

Recommended publications
  • Increased and Mistimed Sex Hormone Production in Night Shift Workers
    Published OnlineFirst March 3, 2015; DOI: 10.1158/1055-9965.EPI-14-1271 Research Article Cancer Epidemiology, Biomarkers Increased and Mistimed Sex Hormone Production & Prevention in Night Shift Workers Kyriaki Papantoniou1,2,3,4, Oscar J. Pozo2, Ana Espinosa1,2,3,4, Josep Marcos2,3, Gemma Castano-Vinyals~ 1,2,3,4, Xavier Basagana~ 1,2,3,4, Elena Juanola Pages 5, Joan Mirabent6,7, Jordi Martín8, Patricia Such Faro9, Amparo Gasco Aparici10, Benita Middleton11, Debra J. Skene11, and Manolis Kogevinas1,2,3,4,12 Abstract Background: Night shift work has been associated with Results: Night workers had higher levels of total progestagens an increased risk for breast and prostate cancer. The effect [geometric mean ratio (GMR) 1.65; 95% confidence intervals of circadian disruption on sex steroid production is a pos- (CI), 1.17–2.32] and androgens (GMR: 1.44; 95% CI, 1.03–2.00), sible underlying mechanism, underinvestigated in hum- compared with day workers, after adjusting for potential con- ans. We have assessed daily rhythms of sex hormones founders. The increased sex hormone levels among night and melatonin in night and day shift workers of both shift workers were not related to the observed suppression of sexes. 6-sulfatoxymelatonin. Peak time of androgens was significantly Methods: We recruited 75 night and 42 day workers, ages later among night workers, compared with day workers (testos- 22 to 64 years, in different working settings. Participants terone: 12:14 hours; 10:06-14:48 vs. 08:35 hours; 06:52-10:46). collected urine samples from all voids over 24 hours on a Conclusions: We found increased levels of progestagens and working day.
    [Show full text]
  • Quantification of Hair Corticosterone, DHEA and Testosterone As
    animals Article Quantification of Hair Corticosterone, DHEA and Testosterone as a Potential Tool for Welfare Assessment in Male Laboratory Mice Alberto Elmi 1 , Viola Galligioni 2, Nadia Govoni 1 , Martina Bertocchi 1 , Camilla Aniballi 1 , Maria Laura Bacci 1 , José M. Sánchez-Morgado 2 and Domenico Ventrella 1,* 1 Department of Veterinary Medical Sciences, University of Bologna, 40064 Ozzano dell’Emilia, BO, Italy; [email protected] (A.E.); [email protected] (N.G.); [email protected] (M.B.); [email protected] (C.A.); [email protected] (M.L.B.) 2 Comparative Medicine Unit, Trinity College Dublin, D02 Dublin, Ireland; [email protected] (V.G.); [email protected] (J.M.S.-M.) * Correspondence: [email protected]; Tel.: +39-051-2097-926 Received: 11 November 2020; Accepted: 14 December 2020; Published: 16 December 2020 Simple Summary: Mice is the most used species in the biomedical research laboratory setting. Scientists are constantly striving to find new tools to assess their welfare, in order to ameliorate husbandry conditions, leading to a better life and scientific data. Steroid hormones can provide information regarding different behavioral tracts of laboratory animals but their quantification often require stressful sampling procedures. Hair represents a good, less invasive, alternative in such scenario and is also indicative of longer timespan due to hormones’ accumulation. The aim of the work was to quantify steroid hormones in the hair of male laboratory mice and to look for differences imputable to age and housing conditions (pairs VS groups). Age influenced all analysed hormones by increasing testosterone and dehydroepiandrosterone (DHEA) levels and decreasing corticosterone.
    [Show full text]
  • Effect of Maternal Intrahepatic Cholestasis on Fetal Steroid Metabolism
    Effect of Maternal Intrahepatic Cholestasis on Fetal Steroid Metabolism Timo J. Laatikainen, … , Jari I. Peltonen, Pekka L. Nylander J Clin Invest. 1974;53(6):1709-1715. https://doi.org/10.1172/JCI107722. Research Article Estriol, estriol sulfate, progesterone, and 17 neutral steroid sulfates, including estriol precursors and progesterone metabolites, were determined in 27 cord plasma samples collected after pregnancies complicated by intrahepatic cholestasis of the mother. The levels of these steroids were compared with those in the cord plasma of 42 healthy controls. In the cord plasma, the steroid profile after pregnancies complicated by maternal intrahepatic cholestasis differed greatly from that seen after uncomplicated pregnancy. Two main differences were found. In the disulfate fraction, the concentrations of two pregnanediol isomers, 5α-pregnane-3α,20α-diol and 5β-pregnane-3α,20α-diol, were high after cholestasis. Other investigators have shown that, as a result of cholestasis, these pregnanediol sulfates circulate in greatly elevated amounts in the maternal plasma. Our results indicate that in cholestasis these steroids cross the placenta into the fetal compartment, where they circulate in elevated amounts as disulfates. Secondly, the concentrations of several steroid sulfates known to be synthesized by the fetus were significantly lower in the cholestasis group than in the healthy controls. This was especially true of 16α-hydroxydehydroepiandrosterone sulfate and 16α- hydroxypregnenolone sulfate. These results suggest that, in pregnancies complicated by maternal intrahepatic cholestasis, impairment of fetal steroid synthesis, and especially of 16α-hydroxylation, occurs in the fetal compartment. Thus, the changes in maternal steroid metabolism caused by cholestasis are reflected in the steroid profile of the fetoplacental circulation.
    [Show full text]
  • Metabolism of Testoterone and Related Steroids in Metastatic Interstitial Cell Carcinoma of the Testis
    Metabolism of testoterone and related steroids in metastatic interstitial cell carcinoma of the testis. M B Lipsett, … , C W Bardin, L M Fishman J Clin Invest. 1966;45(11):1700-1709. https://doi.org/10.1172/JCI105476. Research Article Find the latest version: https://jci.me/105476/pdf Journal of Clinical Investigation Vol. 45, No. 11, 1966 Metabolism of Testosterone and Related Steroids in Metastatic Interstitial Cell Carcinoma of the Testis * M. B. LIPSETT,t G. A. SARFATY, H. WILSON, C. WAYNE BARDIN, AND L. M. FISHMAN (From the Endocrinology Branch, National Cancer Institute, Bethesda, Md.) Interstitial cell carcinoma of the testis is a singu- production rate has been shown to be a conse- larly rare steroid-producing cancer. Of the seven quence of metabolism of dehydroepiandrosterone reported cases (1-7), urinary 17-ketosteroid (17- sulfate. KS) excretion was high in the four cases in which it was measured. Abelson, Bulaschenko, Trom- Methods mer, and Valdes-Dapena (7) fractionated the uri- Routine methods were used to analyze the following: nary 17-ketosteriods and corticoids in one recently urinary 17-KS (8), urinary 17-hydroxycorticoids (9), reported case. There is, however, no comprehen- plasma Silber-Porter chromogens (10), and plasma tes- tosterone (11). sive study of either the production of androgens Gas-liquid chromatography. We carried out gas-liquid or related steroids by this tumor. We have had chromatography (GLC) in a Glowell Chromolab gas the opportunity to study a patient with metastatic chromatograph utilizing a 'Sr ionization detector oper- interstitial cell carcinoma, and we have examined ating at 1,050 v.
    [Show full text]
  • PDG) Enzyme Immunoassay Kit
    DetectX® Pregnanediol-3-Glucuronide (PDG) Enzyme Immunoassay Kit 1 Plate Kit Catalog Number K037-H1 5 Plate Kit Catalog Number K037-H5 Species Independent Sample Types Validated: Dried Fecal Extracts, Urine, Extracted Serum/Plasma, and Tissue Culture Media Please read this insert completely prior to using the product. For research use only. Not for use in diagnostic procedures. www.ArborAssays.com K037-H WEB 210301 TABLE OF CONTENTS Background 3 Assay Principle 4 Related Products 4 Supplied Components 5 Storage Instructions 5 Other Materials Required 6 Precautions 6 Sample Types 7 Sample Preparation 7 Reagent Preparation 8 Assay Protocol 9 Calculation of Results 10 Typical Data 10-11 Validation Data Sensitivity, Linearity, etc. 11-13 Samples Values and Cross Reactivity 14 Warranty & Contact Information 15 Plate Layout Sheet 16 ® 2 EXPECT ASSAY ARTISTRY™ K037-H WEB 210301 BACKGROUND Pregnanediol Glucuronide, C27H44O8, also known as PDG (5β-Pregnan-3a,20a-diol 3-glucosiduronate) is the major metabolite of progesterone1-4. Progesterone is the hormone involved in the female menstrual cycle, gestation and embryogenesis of humans and other species. Progesterone belongs to a class of hormones called progestogens, and is the major naturally occurring human progestogen5,6. Progesterone is an essential regulator of human female reproductive function in the uterus, ovary, mammary gland and brain, and plays an important role in non-reproductive tissues such as the cardiovascular system, bone and the central nervous system. Progesterone action is conveyed by two isoforms of the nuclear progesterone receptor (PR), PRA and PRB. PRA and B are expressed in a variety of normal breast tissue from humans, rats and mice and is also expressed in breast cancer cells7,8.
    [Show full text]
  • Sample Report TST
    PATIENT: Sample Report TEST REF: TST-##-##### TEST NUMBER: ########### RECEIVED: d-mm-yyyy PRACTITIONER: Nordic Laboratories PATIENT NUMBER: ############ TESTED: d-mm-yyyy GENDER: Female COLLECTED: d-mm-yyyy h:m ADDRESS: AGE: 46 DATE OF BIRTH: d-mm-yyyy TEST NAME: Urinary Hormone Metabolites Estrogen Elite Test Name Result Range Urinary Estrogens Estradiol 3.71 H 0.78-1.79 µg/g Cr Premeno-luteal or ERT Estrone 10.95 H 2.27-5.22 µg/g Cr Premeno-luteal or ERT Estriol 3.19 H 0.78-1.98 µg/g Cr Premeno-luteal or ERT E3/(E1+E2) 0 22 L >0.3 (> median value) 2-OH Estradiol 0.77 H 0.17-0.70 µg/g Cr Premeno-luteal or ERT 2-OH Estrone 2.49 0.70-2.54 µg/g Cr Premeno-luteal or ERT 4-OH Estradiol 0.45 H 0.10-0.18 µg/g Cr Premeno-luteal or ERT 4-OH Estrone 0.64 H 0.17-0.47 µg/g Cr Premeno-luteal or ERT 16α-OH Estrone 2 25 H 0.35-1.07 µg/g Cr Premeno-luteal or ERT 2-OH (E1 + E2)/16-α-OH E1 1.45 1.29-5.49 Premeno-luteal or ERT 2-MeO Estradiol 0.11 H 0.03-0.08 µg/g Cr Premeno-luteal or ERT 2-MeO Estrone 0.83 H 0.26-0.68 µg/g Cr Premeno-luteal or ERT 2-MeO E1/2-OH E1 0.33 0.21-0.38 Premeno-luteal or ERT 4-MeO Estradiol 0.02 <0.04 µg/g Cr 4-MeO Estrone 0.05 H <0.04 µg/g Cr 4-MeO E1/4-OH E1 0.08 0.05-0.13 Premeno-luteal or ERT 4-MeO E2/4-OH E2 0.04 L 0.10-0.29 Premeno-luteal or ERT Bisphenol A 1.61 1.11-3.74 µg/g Cr Premeno-luteal Urinary Progestogens Pregnanediol 201 L 465-1609 µg/g Cr Premeno-luteal or PgRT Allopregnanolone 2.05 L 2.23-14.87 µg/g Cr Premeno-luteal or PgRT Pgdiol/E2 54.18 L 1000-1500 (Optimal Luteal Only) Urinary Androgens DHEA 50.43 15.82-129.17 µg/g Cr Premeno-luteal or DHEAT Androstenedione 5.18 3.93-13.53 µg/g Cr Premeno-luteal or ART Testosterone 0.44 L 1.22-3.97 µg/g Cr Premeno-luteal or ART Epi-Testosterone 2.57 2.01-4.66 µg/g Cr Premeno-luteal T/Epi-T 0.17 L 0.5-3.0 5α-DHT 0.12 L 0.28-1.52 µg/g Cr Premeno-luteal or ART Urinary Creatinine Creatinine (pooled) 1 26 0.3-2.0 mg/mL Nordic Laboratories Aps UK Office: Page 1 of 7 Nygade 6, 3.sal • 1164 Copenhagen K • Denmark 11 Old Factory Buildings • Stonegate • E.
    [Show full text]
  • ADVANCED HORMONES (Dried Urine)
    ADVANCED HORMONES (dried urine) Hormone imbalances are associated with numerous symptoms and health conditions. Assessing and diagnosing these changes are important to decrease unnecessary suffering and prevent degenerative diseases. Female hormones fluctuate through a menstrual cycle and at various times of a woman’s life. Imbalances in hormones are associated with PMS, menopause and more complex conditions like PCOS and endometriosis. This test provides a focused overview of the hormonal cascade of both male hormones and female hormones. SYMPTOMS AND CONDITIONS ASSOCIATED WITH HORMONE IMBALANCE PMS Menopause Fertility issues Adrenal stress Endometriosis PCOS Uterine fibroids Fibrocystic breasts Hormonal cancers Osteoporosis Fatigue Insomnia Urinary Hormone Testing Urine testing has the benefit over serum testing that it detects predominantly unbound, active hormones, which are biologically available to their receptors in target tissues. It has a convenient, painless collection procedure that can be performed in the privacy of the home. Urine testing is a stress free, no needles collection that measures metabolic breakdown of hormones. This comprehensive test identifies androgens, female hormones, adrenal hormones and thyroid hormones. ADVANTAGES OF URINARY HORMONE TESTING Measures the free, bioavailable fraction of hormones Measures metabolites of hormones providing a detailed metabolism of hormones Do-it-yourself at home collection offers ease of collection for patient Dried urine test strips correlate with spot or 24 hour urine collection
    [Show full text]
  • Properties and Units in the Clinical Laboratory Sciences Part X
    Pure Appl. Chem., Vol. 72, No. 5, pp. 747–972, 2000. © 2000 IUPAC INTERNATIONAL FEDERATION OF CLINICAL CHEMISTRY AND LABORATORY MEDICINE SCIENTIFIC DIVISION COMMITTEE ON NOMENCLATURE, PROPERTIES AND UNITS (C-NPU)# and INTERNATIONAL UNION OF PURE AND APPLIED CHEMISTRY CHEMISTRY AND HUMAN HEALTH DIVISION CLINICAL CHEMISTRY SECTION COMMISSION ON NOMENCLATURE, PROPERTIES AND UNITS (C-NPU)§ PROPERTIES AND UNITS IN THE CLINICAL LABORATORY SCIENCES PART X. PROPERTIES AND UNITS IN GENERAL CLINICAL CHEMISTRY (Technical Report) (IFCC–IUPAC 1999) Prepared for publication by HENRIK OLESEN1, INGE IBSEN1, IVAN BRUUNSHUUS1, DESMOND KENNY2, RENÉ DYBKÆR3, XAVIER FUENTES-ARDERIU4, GILBERT HILL5, PEDRO SOARES DE ARAUJO6, AND CLEM McDONALD7 1Office of Laboratory Informatics, Copenhagen University Hospital (Rigshospitalet), Copenhagen, Denmark; 2Dept. of Clinical Biochemistry, Our Lady’s Hospital for Sick Children, Dublin, Ireland; 3Dept. of Standardisation in Laboratory Medicine, Kommunehospitalet, Copenhagen, Denmark; 4Dept. of Clinical Biochemistry, Ciutat Sanitària i Universitària de Bellvitge, Barcelona, Spain; 5Dept. of Clinical Chemistry, Hospital for Sick Children, Toronto, Canada; 6Dept. of Biochemistry, IQUSP, São Paolo, Brazil; 7Regenstrief Inst. for Health Care, Indiana University School of Medicine, Indianapolis, Indiana, USA #§The combined Memberships of the Committee and the Commission (C-NPU) during the preparation of this report (1994 to 1996) were as follows: Chairman: H. Olesen (Denmark, 1989–1995); D. Kenny (Ireland, 1996). Members: X. Fuentes-Arderiu (Spain, 1991–1997); J. G. Hill (Canada; 1987–1997); D. Kenny (Ireland, 1994–1997); H. Olesen (Denmark, 1985–1995); P. L. Storring (UK, 1989–1995); P. Soares de Araujo (Brazil, 1994–1997); R. Dybkær (Denmark, 1996–1997); C. McDonald (USA, 1996–1997). Please forward comments to: H.
    [Show full text]
  • Non-Classic Disorder of Adrenal Steroidogenesis and Clinical Dilemmas in 21-Hydroxylase Deficiency Combined with Backdoor Androg
    International Journal of Molecular Sciences Review Non-Classic Disorder of Adrenal Steroidogenesis and Clinical Dilemmas in 21-Hydroxylase Deficiency Combined with Backdoor Androgen Pathway. Mini-Review and Case Report Marta Sumi ´nska 1,* , Klaudia Bogusz-Górna 1, Dominika Wegner 1 and Marta Fichna 2 1 Department of Pediatric Diabetes and Obesity, Poznan University of Medical Sciences, 60-527 Poznan, Poland; [email protected] (K.B.-G.); [email protected] (D.W.) 2 Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, 60-653 Poznan, Poland; mfi[email protected] * Correspondence: [email protected] Received: 3 June 2020; Accepted: 28 June 2020; Published: 29 June 2020 Abstract: Congenital adrenal hyperplasia (CAH) is the most common cause of primary adrenal insufficiency in children and adolescents. It comprises several clinical entities associated with mutations in genes, encoding enzymes involved in cortisol biosynthesis. The mutations lead to considerable (non-classic form) to almost complete (classic form) inhibition of enzymatic activity, reflected by different phenotypes and relevant biochemical alterations. Up to 95% cases of CAH are due to mutations in CYP21A2 gene and subsequent 21α-hydroxylase deficiency, characterized by impaired cortisol synthesis and adrenal androgen excess. In the past two decades an alternative (“backdoor”) pathway of androgens’ synthesis in which 5α-androstanediol, a precursor of the 5α-dihydrotestosterone, is produced from 17α-hydroxyprogesterone, with intermediate products 3α,5α-17OHP and androsterone, in the sequence and with roundabout of testosterone as an intermediate, was reported in some studies. This pathway is not always considered in the clinical assessment of patients with hyperandrogenism.
    [Show full text]
  • Pr REDDY-PROGESTERONE
    PRODUCT MONOGRAPH Pr REDDY-PROGESTERONE Progesterone capsules 100 mg Progestin DIN Owner: Date of Initial Approval: Dr. Reddy’s Laboratories, Inc. April 13, 2017 Princeton, NJ – 08540 USA Date of Revision: Imported By: April 27, 2018 Dr. Reddy’s Laboratories Canada Inc. 2425 Matheson Blvd East, #754 Mississauga, ON L4W 5K4 CANADA Submission Control No: 197711 REDDY-Progesterone Page 1 of 35 Table of Contents PART I: HEALTH PROFESSIONAL INFORMATION ......................................................... 3 SUMMARY PRODUCT INFORMATION ................................................................................. 3 INDICATIONS AND CLINICAL USE ....................................................................................... 3 CONTRAINDICATIONS ............................................................................................................ 3 WARNINGS AND PRECAUTIONS .......................................................................................... 4 ADVERSE REACTIONS .......................................................................................................... 10 DRUG INTERACTIONS ........................................................................................................... 15 DOSAGE AND ADMINISTRATION ....................................................................................... 16 OVERDOSAGE ......................................................................................................................... 16 ACTION AND CLINICAL PHARMACOLOGY ....................................................................
    [Show full text]
  • The Steroid Metabolome in Women with Premenstrual Dysphoric Disorder During Gnrh Agonist-Induced Ovarian Suppression: Effects of Estradiol and Progesterone Addback
    CORE Metadata, citation and similar papers at core.ac.uk Provided by Carolina Digital Repository OPEN Citation: Transl Psychiatry (2017) 7, e1193; doi:10.1038/tp.2017.146 www.nature.com/tp ORIGINAL ARTICLE The steroid metabolome in women with premenstrual dysphoric disorder during GnRH agonist-induced ovarian suppression: effects of estradiol and progesterone addback TV Nguyen1,2,10, JM Reuter1,10, NW Gaikwad3,10, DM Rotroff4,5, HR Kucera3, A Motsinger-Reif4,5, CP Smith4,5, LK Nieman6, DR Rubinow7, R Kaddurah-Daouk8,9 and PJ Schmidt1 Clinical evidence suggests that symptoms in premenstrual dysphoric disorder (PMDD) reflect abnormal responsivity to ovarian steroids. This differential steroid sensitivity could be underpinned by abnormal processing of the steroid signal. We used a pharmacometabolomics approach in women with prospectively confirmed PMDD (n = 15) and controls without menstrual cycle- related affective symptoms (n = 15). All were medication-free with normal menstrual cycle lengths. Notably, women with PMDD were required to show hormone sensitivity in an ovarian suppression protocol. Ovarian suppression was induced for 6 months with gonadotropin-releasing hormone (GnRH)-agonist (Lupron); after 3 months all were randomized to 4 weeks of estradiol (E2) or progesterone (P4). After a 2-week washout, a crossover was performed. Liquid chromatography/tandem mass spectrometry measured 49 steroid metabolites in serum. Values were excluded if 440% were below the limit of detectability (n = 21). Analyses were performed with Wilcoxon rank-sum tests using false-discovery rate (qo0.2) for multiple comparisons. PMDD and controls had similar basal levels of metabolites during Lupron and P4-derived neurosteroids during Lupron or E2/P4 conditions.
    [Show full text]
  • FEMALE HORMONES - Extensive (24 Hour Urine)
    FEMALE HORMONES - Extensive (24 hour urine) Hormone imbalances are associated with numerous symptoms and health conditions. Assessing and diagnosing these changes are important to decrease unnecessary suffering and prevent degenerative diseases. Female hormones fluctuate through a menstrual cycle and at various times of a woman’s life. Imbalances in hormones are associated with PMS, menopause and more complex conditions like PCOS and endometriosis. This test provides a focused overview of the hormonal cascade of both male hormones and female hormones. Hormones and Aging Hormones are powerful molecules that are essential for life. Imbalances of hormones affects other organ systems like the adrenals, thyroid and nervous system. What many people fail to recognise however, is that partial deficiencies also have a wide reaching negative effect on the human body. This is understandable however, when a hormone’s mechanism of action is understood. Aging is one process which is associated with hormone decline. In the past it was thought that this reduction was a normal consequence of the aging process. However, more recently an alternative theory has been proposed; that hormones do not decrease because we age, but rather we age because our hormones decrease i.e. that a reduction in hormonal cellular, genetic and protein control, is the physiological cause of the deterioration of the body which leads to the aging process. It therefore follows that treating hormonal deficiencies (overt, moderate or mild) will prevent or ameliorate disorders associated with the aging process. The treatment of osteoporosis with testosterone and estrogen in men and women is just one example of this.
    [Show full text]