974 NOTES Toxicity of DDT, Dieldrin, Malathion and Fenthion to Rhodnius prolixus in the Laboratory * by IRVING Fox and ILEANA G. BAYONA, School of Tropical Medicine, School of Medicine, University of Puerto Rico, San Juan, Puerto Rico, and HELDA ISABEL OROZCO, Facultad de Medicina, Universidad de Antioquia, Medellin, Colombia Extensive published data on Rhodnius prolixus 11 first-stage and second-stage nymphs brought to cover its distribution and epidemiological role in the School of Tropical Medicine, San Juan, Puerto Chagas' disease in Venezuela," extradomiciliary Rico, by one of us (H.I.O.) on 18 May 1962. These habits,b resistance to practical chemical control specimens originated from a colony in the Facultad efforts,c insect parasites,d life-cycle in the labor- de Medicina, Universidad de Antioquia, Medellin, atory,e, f, ' and effects of radiation on reproduc- Colombia. Concerning this colony, Dr Marcos tion.h, In marked contrast there is a lack of Restrepo I. wrote as follows, in a letter dated toxicological knowledge as derived from laboratory 18 January 1966: tests developed by Busvine & LienJ and by the The strain of Rhodnius prolixus we have in our depart- WHO Expert Committee on Insecticides." It is ment was provided out of one established in BogotA important to obtain this kind of information about from specimens found in various parts of the country, different strains of this serious pest because some especially in the Western Llanos of Colombia and the experts, in their master plans for the control of Department of North Santander. The colony has been Chagas' disease, have contemplated the widespread established for some years (more than 10). The sites of use of insecticides.' The object of this note is to provenance of these Rhodnius had never been sprayed report the results of toxicological tests with DDT, with insecticide in any specific campaign, but in fact we dieldrin, malathion, and fenthion. do not actually know if the inhabitants of the areas employed insecticides at home, which is a common Materials and methods custom among our rural population. The experiments were all made on specimens from Nymphs of the five stages and unsexed adults a laboratory colony. This colony was begun with were subjected to tests. The WHO test kit for determining the susceptibility or resistance of tri- * This investigation was supported in part by Graduate Research Training Grant 5 Ti Al 15 from the National Insti- atomid bugs to insecticides was used according to tute of Allergy and Infectious Diseases, National Institutes the published instructions.k On a single day each of Health, Public Health Service, US Department of Health, Education, and Welfare. concentration of insecticide was tested against all a Romafia, C. (1961) Bol. Ofic. sanit. panamer., 51, 390. stages in two replicates, two more replicates being b Gamboa, C. J. (1963) Bol. Ofic. sanit. panamer., 53, 18. carried out on another day. The controls for all tests c Pifano, F. R., Berti, A. L., Gabald6n, A. & Dfaz Vaz- were first-stage nymphs exposed to paper not quez, L. (1960) Rev. venez. Sanid. Asist. soc., 25, 57. impregnated with insecticide. The required numbers d Ortiz, I. & Alvarez, A. (1959) Rev. venez. Sanid. Asist. of test-tubes mm x 30 were set a soc., 24, 371. (250 mm) in rack e Uribe, C. (1927) J. Parasit., 13, 129. and insecticide-impregnated test paper was inserted f Larrouse, F. (1927) Ann. Parasit. hum. comp., 5, 63. into each one after being folded once lengthwise g Buxton, P. A. (1930) Trans. ent. Soc. Lond., 78 in the shape of a letter V. Ten insects were trans- (Part II), 227. ferred to each tube, which was then covered with h G6mez-Nfifiez, J. C., Gallimore, J. C., Fernandez. M. J. marquisette fabric held by a rubber band: the & Gross, A. (1962) Acta cient. venez., 13, 46. entire rack was placed in the cardboard box of i G6mez-NMfiez, J. C., Gross, A. & Machado, C. (1964) Acta cient. venez., 15, 97. the test kit. The mortality was observed after J Busvine, J. R. & Lien, J. (1961) Bull. Wld Hlth Org., 48 hours for DDT and-dieldrin, but after -24 hours 24, 509. for malathion and fenthion, specimens incapable k WHO Expert Committee on Insecticides (1963) Wid of much movement or to Hith Org. techn. Rep. Ser., 265, 69. unable cling to the £ Pifano, C. F. & Guerrero, L. (1963) Bol. Ofic. sanit. -paper-being counted as dead. The temperature panamer., 53, 396. at the beginning of the tests ranged from 72°F to 1915E TOXICITY OF FOUR INSECTICIDES TO RHODNIUS PROLIXUS 975 TABLE 1 TABLE 2 MORTALITY OF RHODNIUS PROLIXUS AFTER 48 HOURS' MORTALITY OF RHODNIUS PROLIXUS AFTER 24 HOURS' EXPOSURE TO WHO TEST PAPERS EXPOSURE TO WHO TEST PAPERS OF DIELDRIN AND DDT a OF MALATHION AND FENTHION a Insecti- Mortality (%) of Insecti- Mortality / of cde concen- Nymphs of stage: concen- Nymphs of stage tration Adults tration Adults (%) 11 III IV V (%) I I III IV V Dieldrin Malathion 0.2 30 7 5 2 0 20 0.8 67 0 0 0 0 0 0.4 95 50 20 12 0 80 1.6 100 15 0 0 0 0 0.8 100 100 55 25 0 95 3.2 100 90 0 2 0 55 1.6 100 100 72 75 0 100 6.4 100 100 90 25 0 87 12.8 100 100 82 50 0 97 DDT 1.0 0 0 NT b NTb NTb 60 Fenthion 2.0 10 5 0 0 0 95 0.1 2 0 0 0 0 0 4.0 75 35 0 0 0 93 0.2 97 47 2 0 0 0 0.4 100 95 97 10 0 75 a Average of four replicates. b NT = not tested. 0.8 100 92 100 90 10 95 1.6 100 100 100 100 100 100 84°F (220C to 290C) and the relative humidity from 3.2 100 100 100 100 100 100 66% to 86%. a Average of four replicates. Results Control mortality was consistently 0. Table 1 shows that the fifth-stage nymphs were not affected emphasize the importance of following WHO by even the highest concentrations of dieldrin instructions k to use last-stage nymphs in testing and DDT used. However, 95 % of the adults insecticides against triatomid bugs; adults may not succumbed to 2.0% DDT and to 0.8% dieldrin. be substituted for them, and results based on adults First- and second-stage nymphs also were com- alone could be misleading (see, e.g., Table 1). If pletely susceptible to 0.8 % dieldrin, but were sufficient fifth-stage specimens cannot be collected, relatively unaffected by 2% DDT. The results they can be reared from younger specimens or a obtained with malathion and fenthion, shown in colony can be established. The maintenance of Table 2, indicate that fifth-stage nymphs are not susceptible to malathion even at 12.8 % concentra- TABLE 3 tion, and are not very susceptible to fenthion, for a THE STAGES OF RHODNIUS PROLIXUS IN ORDER 1.6 % concentration was required to give 100I% OF RESISTANCE TO INSECTICIDES mortality. However, adults and nymphs of the various other stages, particularly the first and second Insecticide Order of resistance a stages, were more susceptible to these insecticides. (Most resistant - Least resistant) Discussion Dieldrin V IV III Adult II I Table 3 shows that the fifth-stage nymph was the DDT V IV III 11 I Adult most tolerant to all the insecticides tested, and that Malathion V IV III Adult II I adults were more susceptible than third- or fourth- stage nymphs. It seems desirable, then, that all Fenthion V IV III Adult II toxicological studies on this species in the future should include fifth-stage nymphs. The results a I-V refer to the various nymphal stages. 976 NOTES colonies from different geographical areas therefore resistant populations but not a strain resistant to becomes an essential ancillary to toxicological work. both insecticides do not occur in Rhodnius prolixus; It is well known that Triatoma bugs have a high moreover, resistance to chlorinated hydrocarbons natural tolerance to DDT. Triatomna nymphs are and resistance to organophosphorus compounds more tolerant than adults and it is believed that this may both be found in the same population. Fenthion is due to differences in DDT penetration, excretion, was the most effective of the insecticides tried, but and metabolism.m These factors may also account 100 % mortality of fifth-stage nymphs was not for the greater tolerance of Rhodnius nymphs to reached until the next to the highest concentration- DDT. Fifth-stage Rhodnius nymphs apparently have namely, 1.6 °-was used. Evidently, there is need a similar tolerance to DDT, dieldrin and malathion, for further research to find insecticides that are more no deaths occurring at the highest concentration of effective against this insect. insecticide on the WHO impregnated test papers. * * The tolerance therefore seems to be due to physio- logical or anatomical factors responding similarly to The skilful technical assistance of Mr Francisco each insecticide. Apparently, the genetic phenomena Romero-Aleman and Mrs Gisela A. Rivera is gratefully characteristic of some insects that bring about the acknowledged. Thanks are also due to Dr Marcos development of either DDT-resistant or dieldrin- Restrepo I. for information about the original colony, Dr Robert L. Usinger for confirming the determination m Dinamarca, M. L., Agosin, M. & Neghme, A. (1962) of the species, Dr Emesto Osorno Mesa for bibliographi- Exp. Parasit., 12, 61. cal assistance and Miss Juana Arrarte for helpful advice. Observations on the Monitor Lizard, Varanus indicus (Daudin), as a Rat-Control Agent on Ifaluk, Western Caroline Islands* by TERU AKI UCHIDA, Zoological Laboratory, Faculty of Agriculture, Kyushu University, Fukuoka, Japan By gnawing holes in growing coconuts on Pacific my visit to Ifaluk was to report on whether V.