Nprl2 (NM 018879) Mouse Tagged ORF Clone Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for MR205929

Nprl2 (NM_018879) Mouse Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: Nprl2 (NM_018879) Mouse Tagged ORF Clone Tag: Myc-DDK Symbol: Nprl2 Synonyms: 2810446G01Rik; G21; NPR2L; Tusc4 Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin ORF Nucleotide >MR205929 ORF sequence Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC

ATGGGCAGCAGCTGCCGCATCGAATGTATATTCTTCAGCGAGTTCCACCCAACGCTGGGACCCAAAATTA CCTATCAGGTCCCTGAGGATTTCATCTCGCGAGAACTGTTTGACACGGTCCAGGTGTACATCATCACCAA GCCCGAGTTACAGAACAAGCTCATCACTGTCACAGCCATGGAGAAGAAGCTGATTGGCTGCCCCGTGTGC ATTGAACACAAGAAGTACAGCCGCAATGCTCTCCTCTTCAACCTGGGCTTTGTGTGTGACGCTCAGGCTA AGACTTGTGCCCTGGAACCCATCGTTAAAAAGCTGGCCGGCTACTTGACCACGCTGGAGCTTGAGAGCAG CTTTGTATCCAACGAGGAGAGCAAGCAGAAGCTGGTGCCCATCATGACCATCCTGCTGGAAGAGCTGAAT GCCTCCGGCCGCTGCACTCTGCCCATTGACGAATCCAACACCATCCACTTGAAGGTGATCGAGCAGCGGC CAGACCCTCCTGTTGCGCAGGAGTATGATGTGCCCGTCTTTACAAAGGACAAGGAGGATTTCTTCAGCTC GCAGTGGGACCTTACCACACAGCAGATCCTGCCCTACATTGACGGGTTTCGCCATGTCCAGAAGATCTCC GCTGAGGCAGACGTGGAACTCAACCTGGTGCGCATCGCCATTCAGAACCTGCTGTACTATGGTGTTGTGA CACTGGTATCCATCCTCCAGTACTCCAATGTGTATTGCCCAACACCAAAAGTCCAGGATCTGGTGGATGA CAAGTCCCTGCAGGAGGCATGTCTGTCCTACGTCACCAAGCAAGGACACAAAAGGGCCAGTCTCCGAGAT GTGTTCCAGCTATACTGCAGCCTGAGCCCTGGTACCACTGTGCGAGACCTCATCGGCCGCCACCCCCAGC AGTTACAACACGTTGATGAAAGGAAGCTGATCCAGTTTGGGCTGATGAAGAACCTTATCCGGAGACTACA GAAATATCCAGTCCGAGTGTCTCGGGAGGAGCGGAGCCACCCTGCCAGGCTTTACACGGGCTGTCACAGC TATGATGAGATCTGCTGCAAGACAGGCATGAGCTACCATGAGCTGGACGAGCGACTAGAAAATGACCCCA ACATCATCATCTGCTGGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 3 Nprl2 (NM_018879) Mouse Tagged ORF Clone – MR205929

Protein Sequence: >MR205929 protein sequence Red=Cloning site Green=Tags(s)

MGSSCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAMEKKLIGCPVC IEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVSNEESKQKLVPIMTILLEELN ASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKDKEDFFSSQWDLTTQQILPYIDGFRHVQKIS AEADVELNLVRIAIQNLLYYGVVTLVSILQYSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGHKRASLRD VFQLYCSLSPGTTVRDLIGRHPQQLQHVDERKLIQFGLMKNLIRRLQKYPVRVSREERSHPARLYTGCHS YDEICCKTGMSYHELDERLENDPNIIICWK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV

Restriction Sites: SgfI-MluI Cloning Scheme:

Plasmid Map:

ACCN: NM_018879

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 3 Nprl2 (NM_018879) Mouse Tagged ORF Clone – MR205929

ORF Size: 1143 bp OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_018879.1, NP_061367.1 RefSeq Size: 1473 bp RefSeq ORF: 1143 bp Locus ID: 56032 UniProt ID: Q9WUE4 MW: 43.6 kDa Gene Summary: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid- sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function]