bioRxiv preprint doi: https://doi.org/10.1101/2020.01.06.896779; this version posted January 7, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-ND 4.0 International license. 1 Whole genome comparisons of Staphylococcus agnetis isolates from cattle and chickens. 2 3 Abdulkarim Shwani,a Pamela R. F. Adkins,b Nnamdi S. Ekesi,a Adnan Alrubaye,a Michael J. 4 Calcutt,c John R. Middleton,b Douglas D. Rhoadsa,# 5 aCell and Molecular Biology program, University of Arkansas, Fayetteville, Arkansas, USA 6 bDept. of Veterinary Medicine and Surgery, University of Missouri, Columbia, Missouri, USA 7 cDept. of Veterinary Pathobiology, University of Missouri, Columbia, Missouri, USA 8 9 Running Head: S. agnetis phylogenomics 10 11 #Address correspondence to Douglas Rhoads,
[email protected] 12 Keywords: Staphlococcus, Broiler, Mastitis, Lameness, Phylogeny 13 14 Abstract 15 We report the comparisons of genomes of eleven isolates of Staphylococcus agnetis obtained 16 from dairy cattle and chickens. S. agnetis has been previously associated with subclinical or 17 clinically mild cases of mastitis in dairy cattle and is one of several Staphylococcal species 18 isolated from the bone and blood of lame broilers. We have previously shown that a chicken 19 isolate 908 can induce bacterial chondronecrosis with osteomyelitis (BCO) in chickens with 20 incidences of lameness over 50%. We have also assembled and analyzed the genome of isolate 21 908. To better understand the relationship between dairy cattle and broiler isolates, we 22 assembled genomes for S.