Dap. J. Parasit., Vol. 21, No. 5, 318-327, 1972]

On Three Species of the Microphallid Metacercariae Found from a Crab, Hemigrapsus penicillatus with Descriptions of Two New Species (: )

Teiji KIFUNE and YOSHINORI TAKAO

Department of Parasitology, Kurume University School of

Medicine, Ktirume, Japcui

(Received for publication ; August 14, 1972)

It is well known that themicrophallid was removed and the viscera such as gill, metacercariae parasitize in the decapod hepatopancreas, gonad, pericardiac tissues, crustaceans. We recently examined a number etc. were separately examined by compres of marine or brackish water crabs, Hemigra sion between two glass plates. Metacercariae psus penicillatus (de Haan) collected in discovered were accumulated in 0.85% saline Kunisaki-cho, Oita Prefecture, at the eastern and a part of them were subjected to the tip of the Kunisaki Peninsula facing to the morphological observations. The rest meta Japan Inland Sea, and found that those cercariae and several unexamined crabs were crabs harbored three speciesof trematode directly fed to 2 kittens, 6 albino rats, and metacercariae in their hepatopancreas and 6 new born chickens (White Leghorn). pericardiac tissues. A few days after feeding Various hours after feeding, these those metacercariae to kittens, albino rats, were autopsied and their digestive tracts and new born chickens, several gravid adult were dissected in saline for the recovery of worms of three species of the microphallid the grown worms. trematodes were recovered in small intestines of some of those experimental host animals Preparation of the adult worms by autopsy. One species of them is identical All the adult worms obtained from the to Levinseniella conicostoma Bridgman et al., experimental hosts were fixed with Bouin's 1972, which has been recently described fluid, stained with alum carmine, and mo from Shikoku, and other two are new species unted in toto with Eukitt. of the genera Maritrema and Spelotrema as described hereinafter. Results and discussion

Detectio?i of the metacercaria Materials and Methods The crabs were collected four times from Examination of crabs August to November, 1971, and identified as All the crabs were collected by hand near Hemigrapsus penicillatus (de Haan). They the mouth of a small river in Kunisaki-cho, harbored three kinds of the metacercariae Higashikunisaki-gun, Oita Prefecture, and which were distinguishable to one another carried to our laboratory. Those crabs were by their sizes, structure of cyst membranes, usually found from beneath small stones in and habitats in the host crab. Then these shallow pools of the river. After its trans three species were tentatively called A, B, verse length was measured, the dorsal shell and C in order of their sizes. Both the Contributions to the trematode fauna of Japan-IV largest species (A) and the medium-sized one

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(B) were mainly found in pericardiac tissues Morphological observation on the metacercaria and gonads, and their discrimination was The metacercariae obtained are shown in somewhat difficult only by a glance of the Fig. 1. viscera between the glass plates with naked Species A. Mainly parasitic in gonad and/or eyes though it was considerable that the pericardiac tissues. Spherical, 420-430 pt in cyst wall of the species A was composed of diameter. Cyst wall composed of three 3 layers whereas that of the species B was membranes ; the outer one fibrous, 12-15 pt, of 2 layers by the microscopic observation. the middle one hyaline, 15-20 pi, and the Results of the examinations of the crabs are inner one thin, 8-9^, and 30-40 pt in total summarized in Table 1. Some crabs were thickness. In the encysted metacercaria, excluded from the examination as they were conical oral sucker, elliptical acetabulum, and preserved in alcohol or directly fed to the excretory bladder variable in shape conspi experimental hosts. Totally 75.5% (77/102) cuous. Excysted metacercaria quite identical of the crabs examined were found to be para with Levinseniella conicostoma Bridgman, sitized with these metacercariae. Otagaki, Shitanda, et Tada, 1972.

Table 1 Parasitization of the microphallid metacercariae in a crab (Hemigrapsus penicillatus) collected in Oita Prefectire (1971)

No. of crabs Length of Date of parasitized with dorsal shell collection exam (mm) A B C A& A& B & A, B, ined Total only only only B C C & C

Less than 16 30 2 3 7 1 6 1 20

16-20 3 1 2 3 Aug. 29 More than 20 4 1 2 3

Total 34 3 6 7 3 6 1 26

Other 4 exx. not examined (immersed in spirit for specimens)

Less than 16 3 3 3

16-20 26 17 2 3 22 Sep. 6 More than 20 8 4 1 1 6

Total 37 24 3 4 31

Less than 16 6 2 2 4

16-20 12 3 2 3 2 10 Oct. 10 More than 20 1 1 1

Total 19 4 4 5 2 15

Less than 16 4 1 1

19-20 4 1 3 4 Nov. 21 More than 20 1 0

Total 9 1 4 5

Other 50 exx. not examined (directly fed to 3 albinc> rats and 5 chickens)

Grand total 102 3 6 36 7 18 6 1 77

A : Largest species, Levinseniella. B : Medium-sized species, Maritrema. C : Smallest species, Spelotrema. Parasitization rates: A: 29/102(28.4%), B: 20/102(19.6%), C: 61/102(59.8%).

— 33 320

B

100

500^

Fig. 1 Metacercariae found from Hemigrapsus penicillatus. A. Species A, Levinseniella conicostoma Bridgman et al. B. Species B, Maritrema longiforme sp. nov. C. Species C, Spelotrema macrorchis sp nov.

Maximum number in a single host was 20. Species C. Parasitic in hepatopancreas. Species B. Mainly parasitic in gonad or Spherical, 135-140 ft in diameter. Cyst wall neighboring tissues. Spherical, 270-275 ,« in composed of two thin membraneous layers ; diameter. Cyst wall composed of two mem the outer one fibrous, 2-3//, and the inner braneous layers ; the outer one fibrous, the one hyaline, 5-7 fi, and 5-8 fi in total thi inner hyaline, both 10-12 ft and 20-22// in ckness. Only oral sucker conspicuous, other total thickness. Both of elliptical suckers organs generally inconspicuous except for conspicuous, excretory bladder elliptical, lon testes finely visible. gitudinally elongated. Testes also finely Maximum number in a single host was 118. conspicuous. Excysted metacercaria chara cteristic in the distribution of vitellaria Recovery of adults from the experimental hosts encircling the genital organs in the posterior Out of 14 animals fed with the metacerca half of the body and easily identified as riae, 1 kitten, 2 albino rats, and 1 chicken Maritrema sp. which were autopsied between 22 and 44 Maximum number in a single host was 14. hours after feeding were found to harbor grown adult worms in their small intestines.

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Table 2 Number of adult worms recovered from experimental hosts.

Duration till No. of worms recovered Host No. autopsy — Total ihrs) Levinseniella Maritrema Spelotrema

1 22 1* 2(1) 2(1) Kitten 2 140

1 24 7 2 ! 13

2 40, 19, 17* 6 i 7 3 115, 88, 86* 0 Albino rat 1 168 0

5 n 0

6 it 0

1 19 1)

2 44 0

3 44, 24* 3 Chicken i 52, 32* 0

5 76, 56* 0

6 96 0

Total 18 25

* Metacercariae were fed two or three times. In the parentheses shows the number of unexcysted metacercaria found from stomach.

Other 10 animals which were sacrificed earlier till the autopsy of the hosts. In adequate ■or later than the above-mentioned durations natural hosts, these adults may be able to ■of the infection were not parasitized. Results parasitize for more prolonged durations and are shown in Table 2, in which the hosts to contain much more eggs so as to fill up •are arranged in order of the lengths of the their hindbody. durations. No. 1 of the kittens harbored 2 mature but ungravid adults of Levinseniella Adult in small intestine and 1 unexcysted meta All the adult worms were mounted in toto. cercaria of the same species in stomach. The species A is quite identical with Levins The adults apparently originated from those eniella conicostonia Bridgman et al. of which fed 22 hours before and the unexcysed the metacercaria is known to be parasitic in metacercaria was from those fed 1 hour the same crab also in Kagawa Prefecture, before the autopsy. No. 1 of the albino Shikoku, and the adult was found from knot rats harbored 13 worms of all the three and curlew. This is harbored by all the species. From these materials, the species experimental host species in the present A, B, and C in the metacercarial stage are study. The species B was found only in the identified as Levinseniella conicostonia, Mari albino rat No. 1 (2 exx. only) and is a new trema sp., and Spelotrema sp., respectively. species of the Maritrema. The species Maritrema was more abundant than Levins C was found from the albino rats Nos. 1 eniella in the metacercarial stage but only 2 and 2 (5 exx. together) and is also a new adults of the former were recovered from species belonging to the genus Spelotrema. the experimental hosts whereas 18 of the latter were obtained. All the adults had a Description of the adults few eggs in their uterine ducts. This may Levinseniella conicostonia Bridgman, Ota- •depend on short durations of the infection gaki, Shitanda, et Tada, 1972

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s B

Fig. 2 Adults of the microphallid trematodes obtained from the albino rat no. 1 (exp. host), ventral view. A. Levinseniella conicostoma Bridgman et al. (slide no. 247) B. Maritrema longiforme sp. nov., holotype (slide no. 256-b) C. Spelotrema macrorchis sp. nov., holotype (slide no. 257-a)

Shikoku Christ. Coll. Treat. 23 : 46, fig. 5, characters are not recapitulated here. Only 1972 the result of our measurements is compared In the present feeding experiment, totally with the original one as shown in Table 3. 18 specimens were obtained ; 2 exx. from a kit Though both two specimens obtained from ten (No. 1), 7 and 6 exx. from 2 albino rats the kitten were ungravid, they were equally (Nos. 1 and 2), and 3 exx. from a chicken (No. measured because they seemed to be full 3) as shown in Table 2. All specimens were grown from the sizes of body and other gravid with comparatively many eggs and organs. are quite identical with the headlined species. Distribution: Shikoku (Kagawa Pref.) (loc. This was originally described with 20 spe cit.) and Kyushu (Oita Pref.) (new record). cimens obtained from Calidris canutus and Definitive hosts : Calidris canutus Linnaeus Numenius madagascariensis collected in Kaga- (prohably subsp. rogersi (Mathews)), Num wa Prefecutre, Shikoku. Becauset he original enius madagascariensis (Linnaeus), mouse (ICR description is so excellent that the diagnostic strain) (exp.), albino rat (Wister strain) (exp.)

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Table 3 Comparison of measurements in Levinseniella conicostoma

Authority Bridgman et al., 1972 Present record Locality Kagawa Pref., Shikoku Oita Pref., Kyushu No. of 20 (from Calidris canntus 18 (2 from a kitten, 13 from specimens and Niimenius mada- 2 albino rats, and 3 from a examined gascariensis) chicken, exp.)

0.0800 (sic!)-1.120 (0.942) Body 0.940-1.450 0.235 -0.280 (0.250) 0.270-0.470 0.1125-0.1350 (0.1227) Oral sucker lh 0.095-0.160 \W 0.0650-0.0825 (0.0770) 0.070-0.110

Prepharynx L 0.0250-0.0675 (0.0480) 0.017-0.136

(L 0.0300-0.0375 (0.0333) 0.023-0.047 Pharynx VW 0.0250-0.0325 (0.0297) 0.025-0.043

Oesophagus L 0.225 -0.350 (0.274) 0.250-0.400

Ceca ., L 0.150 -0.180 (0.178) 0.160-0.290

(L 0.065-0.110 Acetabulum D 0.0660-0.0725 (0.0670) VW 0.050-0.100 /Left (L °-060-°-170 0.0425-0.0750 (0.0608) LeU VW 0.060-0.120 Testes Iw 0.0625-0.0900 (0.0762) L-h. (L 0.055-0.120 ■Rlght IW 0.055-0.130

0.0450-0.0625 (0.0527) 0.060-0.130 Ovary [w 0.0550-0.0950 (0.0725) 0.065-0.135

0.0225-0.0250 (0.0237) 0.021-0.028 Uterine eggs (L \w :0.0125-0.0150 (0.0141) 0.014-0.018

Metacercaria D '0.390 -0.415 0.420-0.430 Thickness of 0.019O-0.0250 0.012-0.015 outer layer 0.0050-0.0075 0.015-0.020 middle layer 0.0010-0.0020 0.008-0.009 inner layer

D : Diameter L : Length W : Width

{loc. cit.); Fells catis Linnaeus (juvenile) (exp.), cone-shaped. Ceca shorter than eso and Gallus gallus v. domesticus (Brisson) phagus. (white leghorn, juvenile) (exp.) (new records). • • L. conicostoma Bridgman et al., 1972 Habitat: Small intestine. Remarks: There is another apparently . -First intermediate host : Unknown. distinguishable unnamed species reported by Second intermediate host : Hemigrapsus Miyazaki from Japan. It is allied to L. penicillatus (de Haan) {loc. cit.)-habitat : hepa- bucephalae but differs from the latter by its topancreas {loc. cit.) and pericardiac tissues larger sizes and more elliptical shapes of and gonad (new records). testes and ovary. Komiya (1965) transferred There are two species of the genus Levin this species to the genus Gynaecotyla but it seniella in Japan and those are distinguished is incorrect. He may be affected by the by the following key. transfer of Levinseniella squatarolae to Gynae cotyla by Yamaguti (1939). G. squatarolae Key to the Japanese species of the genus and Levinseniella sp. of Miyazaki are und Levinseniella Stiles et Hassall oubtedly not congeneric. 1. Oral sucker ordinary, not protruded ante Maritrema longiforme sp. nov. riorly. Ceca longer than esophagus L. bucephalae (Yamaguti, 1935) Measurements are based on two gravid Oral sucker distinctly protruded anteriorly, specimens obtained from the albino rat No.

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1, which was autopsied 24 hours after feeding There are at least 7 species of the genus- the metacercariae. No other animals harbo Maritrema in Japan and those are distin red this species. guished by the following key. Definitive host : albino rat (Wister strain) Key to the Japanese species of the genus (exp.). Maritrema Nicoll Habitat : Small intestine. Body elongate fusiform, 0.89-1.04 mm in 1. Ovary more or less lobed. 2 length, broadest at level of cecal termina - Ovary not lobed, usually elliptical. • • • -4 tions, 0.25-0.405 mm. Integument of the 2. Posterior part of vitellaria not interrupted anterior half of body spined. Oral sucker medially. M. eroliae Yamaguti, 1939 elliptical, broader than long, 60X65-80^. Posterior part of vitellaria interrupted Prepharynx short but conspicuous, 21-25 ft medially. 3 in length. Pharynx globular, 26-29X22-27 fi. 3. Large species, body length more than 1.0 Esophagus slender, long, 210-270 fi in length. mm. Metacercaria parasitic in crab. • • • • Ceca shorter than esophagus, 160-210 fi in M. urayasense Ogata, 1951^ length ; cecal terminations located at level - Small species, body length less then 0.6- of the anterior margin of acetabulum. mm. Metacercaria parasitic in shrimp. •• Acetabulum larger than oral sucker, 105-125 M. kitanense Shibue, 1953 X 85-90 fi, located medially in the middle of 4. Body pyriform, testes separated to each body. Ovary elliptical, near the right lateral other by a distance longer than their own margin of body and at just side of aceta- diameter. blum, 100-110 X 70-85 fi. Testes symmetrical, M. macravestibulum Ogata, 19472) elliptical, almost equal to each other in size - Body elongated, longer than twice of and shape, left testis 100-110x85-90 ft, and breadth. 5 right one 90-100x80-90^. Cirrus pouch 5. Prepharynx conspicuous; vitellaria bom- arched, anteriorly convex, located just ante pletely encircling testicular and uterine rior to acetabulum. Vitellaria distributed area. 6 near the margin of hindbody and encircling - Prepharynx short ; vitellaria clearly inter the uterine and testicular area, not closing rupted in the anterior part. at the anterior part of it on the median M. Ion giforme sp. now line. Egg small, elliptical, 23-29x11-14^, 6. Ovary at the right side of acetabulum operculated. and subequal to testes in size. This is allied to but distinguishable from M. setoenense Bridgman, 1971 the following congeneric species : M. linguilla - Ovary behind acetabulum and much larger Jagerskiold by its much smaller size ; M. than acetabulum ; testes greatly larger opisthometra Lenov by its much larger cirrus than ovary. pouch and testes ; long prepharynx, and loca M. opisthometra Leonov, 19583) tion of ovary being behind acetabulum ; M. Spelotrema macrorchis sp. nov. prosthometra Deblock et Heard by its long prepharynx and ceca, elongated cirrus pouch, Measurements and description are based and large testes ; M. setoenense Bridgman by 1) This was synonymized with M. ei'oliae Yama its long prepharynx and large cirrus pouch ; guti by Bridgman (1971). We agree with his and M. laricola Ching by its small size, long synonymization but reserve to seject this prepharynx, interiorly distributing vitellaria. species in the present paper. 2) This has been often incorrectly cited as 1946 Type materials: 1 holotype (Fig. 3B) and by many authors including Ogata himself, 1 paratype (slide no. 256). but the publication date on the cover of the Type depository : The type materials are reprint is read as 30 March 1947. preserved in the Department of Parasitology, 3) This has been added to the Japanese fauna Kurume University School of Medicine. by Bridgman et al. (1972).

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on five specimens obtained from the albino been at least 3 species and 1 subspecies of rats nos. 1 and 2, which were autopsied 24 the genus Spelotrema in Japan. Adding the and 17-19-40 hours after feeding the meta- species described in the present paper, the cercariae. No other animals harbored this 5 species of the genus occurring in Japan species. are distinguishable by the following key. Definitive host : albino rat (Wister strain) Key to the Japanese species of the genus (exp.). Spelotrema Jagerskiold Habitat : Small intestine. Body oval to fusiform, 0.61-0.735 mm in 1. Seminal vesicle very large, larger than length ; broadest at level of testis, 0.36-0.42 acetabulum, almost completely occupying mm in breadth. Integument of the anterior the intercecal space in front of aceta two-thirds of body spined. Oral sucker term bulum. • . 2 inal, elliptical, slightly transversely elongated, - Seminal vesicle subequal to acetabulum in size. 3 50-70X 70-80/i. Prepharynx short, occasional 2. Ovary transversely elongated. ly inconspicuous, 0-7 // in length. Pharynx slightly transversely elongated, elliptical, 25- S. capellae Yamaguti, 1939 - Ovary almost circular. 32X36 //. Esophagus slender, long, 140-190// in length. Ceca slightly longer than esopha S. asadai (Kaji, 1957)n 3. Body elongated, prepharynx longer than gus, 190-200// in length, slightly anteriorly the half longth of esophagus. anteriorly convex. Acetabulum subequal to S. longicollis Yamaguti, 1939 oral sucker, 60-70 X 65-75 //. Testes sym - Body pyriform, prepharynx short. Ante metrical, large, elliptical, obliquely situating rior part of uterus reaching in front of in the third quarter of the body ; right testis testes, overlapping the cecal terminations. 110-150 X 90-170 //; left testis 130-165x100- 170//. Genital pore cuticularized so as to 4 4. Moderate-sized species, length about 0.7 form a sucker-like structure, 29-34x36-38//. mm. Metacercaria parasitic in crab. • • • • Ovary elliptical, dextroanterior to acetabulum, S. macrorchis sp. nov. 70-95X70-120//. Seminal vesicle elliptical, - Small species, length less than 0.6 mm. sinistroanterior to acetabulum, 60-77x27-41 Metacercaria parasitic in shrimp. //. Prostatic cells not so numerous, located S. minus (Ochi, 1928) near genital pore. Uterus occupying the posterior space of hindbody ; a part of it 1) This species was originally described as Micro- distributed a little front of testis through a phallus minus var. asadai by Kaji (1957). narrow gap between testis and lateral margin The nominotypial species, M. minus, was later transferred to the present genus, Spelo of body in both sides. A few eggs contained trema, by Yamaguti (1958), and subsequently in uterus probably because of a short duration var. asadai was also transferred to the same of the experimental infection. Egg small, genus with an alteration to the subspecific elliptical, 21-25x9-11//, operculated. category of S. minus by Yamaguti (1971). This is allied to S. pygmaeum (Levinsen), According to the original description of the the generotype, but differs from it by large variety, however, it is sympatric with the sizes of body and testes, on the contrary nominotypical species and has enough differ small seminal vesicle. ences to be separable specifically with the Type materials : 1 holotype (Fig. 3C) (slide latter. We, therefore, elevate the variety (or subspecies by Yamaguti) to the specific rank no. 257) and 4 paratypes (slide nos. 257-259 ; as follows. each slide contains two specimens together). Type depository : The type materials are Spelotre?na asadai (Kaji, 1957) preserved in the Department of Parasitology, Microphallus minus var. asadai Kaji, 1957 Kurume University School of Medicine. Tokyo Iji Shinshi, 74 : 270, figs. 1-2. According to Yamaguti (1971), there have Spelotrema minus asadai Yamaguti, 1971

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Synopsis of digenetic trematodes of ver References tebrates, 1 : 781.

1) Belopol'skaia, M. M. (1952) : Trematody Summary cemeistva Microphallidae Travassos, 1920. Three species of the microphallid trema In : Skrjabin, K. I. (ed.) : Trematody Zhibot- todes are obtained from a few experimental nykh i Cheloveka (Osnovy Trematodologii), host animals orally infected with metacerca- 6, 619-756, Akad. Nauk SSSR, Moskva. (In Russian) riae found from a crab, Hemigrapsus penicil- 2) Belopol'skaia, M. M. (1963) : Cemelstvo latus (de Haan), collected in Kunisaki-cho, Microphallidae Travassos, 1920. In: Skrjabin, Oita Prefecture, Kyushu. Two of them are K. I. (ed.) : Trematody Zhibotnykh i Chelo new to science and described on the basis veka (Osnovy Trematodologii), 21, 259-502, of several specimens obtained from albino Akad. Nauk SSSR, Moskva. (In Russian) rats under the names of Maritrema longlforme 3) Bridgman, J. F. (1971) : The life cycle of sp. nov. and Spelotrema macrorchis sp. nov. Maritrema setoe?iensis n. sp. (Trematoda : Another one is identical as Levinseniella Microphallidae). Jap. J. Parasit., 20, 13-23. conicostoma Bridgman et al., 1972. This is 4) Bridgman, J. F., Otagaki, K., Shitanda, I., a new distributional record and the domestic and Tada, K. (1972) : Nine metacercariae cat (kitten) and the new born chicken (White (Microphallide : Trematoda) from arthropods of the Japan Inland Sea, Kagawa Prefecture, Leghorn) are added as its experimental including the description of Levinseniella hosts. conicostoma n. sp. Shikoku Christ. Coll. M. longlforme is closely related to M. Treat., 23, 35-61. setoenense Bridgman, 1971, but differs from 5) Chen, H.-t. (1956) : Studies on Chinese it by elongated body, anteriorly interrupted microphallid trematodes of the subfamily vitellaria, short prepharynx, etc. S. macror Microphallinae (Trematoda : Microphallidae). chis is allied to the generotypical species, S. Acta zool. sin., 8, 49-59, pis. 1-3. pygmaeum (Levinsen, 1881), but easily dist 6) Ching, H. L. (1963) : The description and inguished with large sizes of body ane testes. life cycle of Maritrema laricola sp. n. (Trema Keys to the Japanese species of the above- toda : Microphallidae). Canad. J. Zool., 41, mentioned three genera are given at the 881-888. ends of the descriptions of each species. 7) Coil, W. H. (1955) : Notes on the genus Spelotrema minus asadai (Kaji, 1957) is eleva Maritrema Nicoll, 1907 (Trematoda : Micro phallidae) with the description of two new ted to the specific rank because it is quitely species. J. Parasit., 41, 533-537. sympatric to and different from the nomino- 8) Deblock, S., et Combes, C. (1965): Contri typical species, S. minus (Ochi, 1928). bution a l'etude des Microphallidae, Travas

Acknowledgements sos, 1920 (Trematoda). X. Maritrema pyren- aica n. sp., parasite de Desman (Mammifere We express our gratitude to Dr. John F. Brid Insectivore). Essai de cle diagnostique des gman, Shikoku Christian College, for his kind especes du genre. Bull. Soc. Zool. Fr., 90, communication, to Dr. Hisakatsu Minei, Kyushu . 101-117. University, for the identification of the carb, and 9) Deblock, S., et Heard, R. W. (1969): Con Messrs. Sumihisa Kamachi and Nobuhito Kojo, tribution a l'etude des Microphallidae Kurume University, for their helps in the col Travassos, 1920 (Trematoda). XIX. Description lections and examinations of the crab. Thanks de Maritrema prosthometra n. sp. et de Lon- are also due to Prof. Koyo Okabe, Kurume gidnctotrema nov. gen. parasites d'Oiseaux Univereity, for his constant guidance and encoura Ralliformes d'Amerique du Nord. Ann. Para gements through the present study. sit. hum. comp., 44, 415-424. The abstract of the present paper was read 10) Deblock, S., et Rausch, R. L. (1968) : Con before the 41th Annual Meeting of the Japanese tribution a l'etude des Microphallides Trvas- Society of Parasitology held in April 1972 in sos, 1920 {Trematoda). XV. Description de Okayama. Maritrema megametrios n. sp. parasite d'Ois-

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ケフサイソガニに見出された新種を含むMicrophaUidae 被嚢幼虫３種について

木船悌嗣，高尾善則 (久留米大学医学部寄生虫学教室）

大分県東国東郡国東町で採取したケフサイソガニ 主として追加された． ＨｾﾉﾉZigγ⑫sz`ｓ〆"iciZmZ"ｓ（deHaan）に見出した被嚢 ＭＺｏ'２９i/bγ"ｚｅはMsGZoど"ど"seBridgman，１９７１ 幼虫を数種の実験動物に経口感染させてMicrophal‐ に似るが，体形はより細長く，卵黄腺は前半中央部で lidae吸虫３種の成虫を得た．うち２種は新種と認めら 明瞭に分離すること，前咽頭が甚だ短いことなどで区別 れたので）ラットより得た標本をもとに，それぞれ できる．Ｓ､，"αcγoγcﾉｶﾉｓは属の模式種Ｓ・〃9ｍαα`"ｚ Ｍｚγi〃emaZo"gi/ｂｒｍｅおよびSPeJoZγe"Ｚａｍａｃγoγcﾙﾉｓ (Levinsen，1881）に似るが，はるかに大型で，体内各器 と命名記載した．残る１種は最近香川県から発見された 官とくに睾丸が著しく大きいことで容易に区別できる． Ｌｅ℃i"se"ieZmco"icostomaBridgmanetaZ.，１９７２と同 なお上記３属の邦産種のための検索表をそれぞれの種の 定されたが,｢大分県は新しい分布記録となり）また同時 記載の末尾に添えた.〕またSPeZot'．e'"α''z伽ｓａＳＭｚ/ に子猫と初生雛（白色レグホン）とが新しい実験的終宿 (KajL1957）を種に昇格せしめた．

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