DOCSLIB.ORG

Cytogenetic and Molecular Characterization of Lacertid Lizard Species from the Iberian Peninsula", Presentado Por Dña

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Cytogenetic and Molecular Characterization of Lacertid Lizard Species from the Iberian Peninsula Cytogenetic and molecular characterization of lacertid lizard species from the Iberian Peninsula Verónica Rojo Oróns Doctoral Thesis 2015 Supervisors: Dr Horacio Naveira Fachal, Dr Andrés Martínez Lage Department of Cell and Molecular Biology D. HORACIO NAVEIRA FACHAL, DOCTOR EN BIOLOGÍA Y CATEDRÁTICO DE UNIVERSIDAD DEL ÁREA DE GENÉTICA DEL DEPARTAMENTO DE BIOLOGÍA CELULAR Y MOLECULAR DE LA UNIVERSIDADE DA CORUÑA, Y D. ANDRÉS MARTÍNEZ LAGE, DOCTOR EN BIOLOGÍA Y PROFESOR TITULAR DE UNIVERSIDAD EN EL ÁREA DE GENÉTICA DEL DEPARTAMENTO DE BIOLOGÍA CELULAR Y MOLECULAR DE LA UNIVERSIDADE DA CORUÑA, INFORMAN QUE el trabajo titulado "Cytogenetic and molecular characterization of lacertid lizard species from the Iberian Peninsula", presentado por Dña. VERÓNICA ROJO ORÓNS para optar al título de Doctora en Biología con Mención Internacional por la Universidade da Coruña, ha sido realizado bajo nuestra dirección. Considerándolo finalizado, autorizamos su presentación y defensa. A CORUÑA, A 22 DE SEPTIEMBRE DE 2015 Fdo. Dr. Horacio Naveira Fachal Fdo. Dr. Andrés Martínez Lage Fdo. Verónica Rojo Oróns To my dear Rodri To my granny Acknowledgements Firstly, I would like to thank my supervisors, Horacio Naveira and Andrés Martínez, for trusting me and for the opportunity to work on such an interesting project. Thank you for your patient, guidance, and for sharing your knowledge and experience with me! Many thanks to Ana González and Pedro Galán for their significant contributions to this research. Ana, thanks for your friendship and all your support in scientific and non-scientific questions. Pedro, thank you for collecting all the specimens for this thesis, and for your invaluable wealth of knowledge about lacertids. I am very grateful to all the people who generously hosted me during my fruitful internships abroad: Ettore Olmo, Vincenzo Caputo Barucchi, Massimo Giovannotti, Paola Nisi Cerioni, Andrea Splendiani and Paolo Ruggeri, at the Universitá Politecnica delle Marche (Ancona); Malcolm Ferguson-Smith, Jorge Pereira, Fumio Kasai and Willem Rens, at the University of Cambridge. These research internships have been an exciting learning experience, and essential for the completion of this thesis. My special thanks to Massimo, for his infinite patient to show me the tips and tricks of cell cultures and FISH. Thank you for your support and valuable advice over all these years. Thanks also to Adriana Canapa and Marco Barucca for their warm welcome, and to Çinel, Bruna, Sara and Sabrina, for our good moments in Ancona. Back to Coruña, thanks to my colleagues at the Evolutionary Biology Group and, especially, to my wonderful lab partners (past, present, and newcomers): Álex, David, Joaquín, Rosa, my Panoquita, Elba, Neus, Nuria, Luis, Iván, Inés, Eva...I’ve been really lucky to work with you! Thank you very much for the many good times we enjoyed (and I’m sure we will still enjoy) in and outside the lab. Many thanks to Rosa García Díaz and her magic hands, for their brilliant technical skills, and to Nani, Raquel, and Miriam, from the University Research Support Services, for their willing assistance on DNA sequencing. I would also like to thank the great AllGenetics team and, especially, Joaquín, Álex and Antón, for giving me the opportunity to work and learn from them. To my friends (you know who you are), for being always there and for helping me forget about everything when I needed it. And, of course, for your interest in this lizards’ "thing"! I am forever grateful to my amazing family. To Rosa, Pedro, and Lucas, for their energizing support throughout this odyssey, and for bringing back happiness into our lives. To Rubén; thank you for your incredible patient and understanding, and for always making me end the day with a smile. To Cris, for sharing with me a bit of her talent and making this beautiful cover design. And, especially, to my parents, for their unconditional love, encouragement and support. This thesis, and everything I achieved, is thanks to you. Finally, I wish to thank the thesis committee and the external reviewers who read and commented the draft of this thesis report. I was supported by a FPU (Formación de Profesorado Universitario) grant, from the Spanish Ministry of Education, Culture and Sport, and by a Research Internship Grant, from the Universidade da Coruña. Contents Abbreviations v Short abstracts ix Extended abstract (in Spanish) xiii Introduction 1 1. Reptilian karyotype evolution 3 1.1 Chromosome numbers and karyotypic structure 3 1.2 Conservation of chromosomal synteny 5 2. The evolution of sex-determining mechanisms in reptiles 7 2.1 The evolution of sex chromosomes 8 2.1.1 Long-term preservation of homomorphic sex chromosomes 10 2.2 Sex chromosomes in reptiles 11 2.3 Multiple origins of reptilian sex chromosomes 16 2.4 Turnover of sex chromosomes vs. the evolutionary trap hypothesis 17 3. Satellite DNA: features and evolution 20 4. The study species 22 4.1 The family Lacertidae 22 4.2 The genus Iberolacerta 23 5. References 27 Aims and objectives 45 Chapter I: Karyological characterization of the endemic Iberian rock lizard, Iberolacerta monticola (Squamata, Lacertidae): insights into sex chromosome 49 evolution Chapter II: Comparative chromosome painting in lacertid lizards: 81 highly conserved karyotypes but independent origins of sex chromosomes? Chapter III: Evolutionary dynamics of two satellite DNA families in Rock lizards of the genus Iberolacerta (Squamata, Lacertidae): different histories but 127 common traits Conclusions 173 Annexes 177 Annex I: Karyological characterization of the endemic Iberian rock lizard, 179 Iberolacerta monticola (Squamata, Lacertidae): insights into sex chromosome evolution. Cytogenetic and Genome Research 2014; 142: 28–39 Annex II: Isolation and characterization of two satellite DNAs in some 193 Iberian rock lizards (Squamata, Lacertidae). Journal of Experimental Zoology Part B Molecular and Developmental Evolution 2014; 322B: 13–26. Annex III: Evolutionary dynamics of two satellite DNA families in Rock 209 lizards of the genus Iberolacerta (Squamata, Lacertidae): different histories but common traits. Chromosome Research (in press) Abbreviations 2n Diploid chromosome number 4xT 4x SSC, 0.05% Tween-20 μL Microlitre μm Micromolar ºC Degree Celsius Nucleotide diversity ACSL1 Acyl-CoA synthetase long-chain family member 1 ADAM12 ADAM Metallopeptidase Domain 12 Ag-staining Silver-staining ATP2A2 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 ATP5A1 ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle BLAST Basic Local Alignment Search Tool C19orf47 Chromosome 19 open reading frame 47 CCD Charge coupled device CDH8 Cadherin 8, type 2 CGH Comparative genomic hybridization CMA3 Chromomycin A3 COL5A1 Collagen, type V, alpha 1 Cy3 Cyanine 3 DAPI 4′,6-Diamidino-2-phenylindole DDBJ DNA DataBank of Japan DMRT1 Doublesex and mab-3 related transcription factor 1 DNA Deoxyribonucleic acid DNase Deoxyribonuclease dNTP Deoxynucleotide triphosphate v DOP-PCR Degenerate oligonucleotide primer–polymerase chain reaction dUTP 2'-Deoxyuridine 5'-triphosphate EEF2 Eukaryotic elongation factor 2 EMBL European Molecular Biology Laboratory ESD Environmental sex determination FBXW11 F-Box And WD Repeat Domain Containing 11 FCA Factorial correspondence analysis FISH Fluorescence in situ hybridization FITC Fluorescein Iso-Thyocianate g Relative centrifugal force (RCF) GMPPA GDP-mannose pyrophosphorylase A GSD Genotypic sex determination h Hour HEPES 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid iCGH Interspecies comparative genomic hybridization IgG Immunoglobulin G IPO5 Importin 5 ITS Interstitial telomeric site IUPAC International Union of Pure and Applied Chemistry KCl Potassium chloride LLitre MMolar mg Milligram MgCl2 Magnesium Chloride min Minute mL Millilitre mM Millimolar vi mmol Millimol mya Million years ago MYST2 MYST histone acetyltransferase 2 myr Million years NaCl Sodium Chloride ng Nanogram NCBI National Center for Biotechnology Information NOR Nucleolar organizing region OAF Out at first homolog OCA2 Oculocutaneous Albinism II OSGIN1 Oxidative stress induced growth inhibitor 1 PCR Polymerase chain reaction PIK3CD Phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit delta RAD-seq Restriction size-associated DNA sequencing RNase Ribonuclease RNF19B Ring finger protein 19B rRNA Ribosomal ribonucleic acid s Second satDNA Satellite DNA SBNO1 Strawberry notch homolog 1 (Drosophila) SH3PXD2A SH3 And PX Domains 2A SKIL SKI-Like Proto-Oncogene SNP Single nucleotide polymorphism SOX5 (Sex determining region Y)-box 5 SS18 Synovial sarcoma translocation, chromosome 18 SSC Saline sodium citrate TKT Transketolase vii TOP2A Topoisomerase (DNA) II alpha TRIM37 Tripartite motif containing 37 Tris Tris(hydroxymethyl)aminomethane TRITC Tetramethylrhodamine TSD Temperature-dependent sex determination UV Ultraviolet WAC WW domain containing adaptor with coiled-coil WDR43 WD Repeat Domain 43 WT1 Wilms Tumor 1 ZNF326 Zinc Finger Protein 326 viii SHORT ABSTRACTS Abstract Reptiles, with their great diversity of sex-determining systems, have long been regarded as a model group for studying the evolution of sex determination and sex chromosomes. They also hold a key phylogenetic position to elucidate the organization and evolution of amniote genomes. This PhD thesis aims to contribute to this understanding by investigating sex chromosomes and karyotype evolution in lacertid lizards, with a focus on rock lizard species (genus Iberolacerta)
Load more

Recommended publications
  • (Lacerta Agilis and Zootoca Vivipara) in Extensively Used Farmland In
    Folia biologica (Kraków), vol. 56 (2008), No 3-4 doi:10.3409/fb.56_3-4.165-171 DensitiesandMorphologyofTwoCo-existingLizardSpecies (Lacertaagilis and Zootocavivipara)inExtensivelyUsedFarmland inPoland* AnnaEKNER,IgorMAJLÁTH,ViktóriaMAJLÁTHOVÁ, Martin HROMADA, Martin BONA, MarcinANTCZAK,MaciejBOGACZYK andPiotrTRYJANOWSKI Accepted April 22, 2008 EKNER A., MAJLÁTH I., MAJLÁTHOVÁ V., HROMADA M., BONA M., ANTCZAK M., BOGACZYK M., TRYJANOWSKI P. 2008. Densities and morphology of two co-existing lizard species (Lacerta agilis and Zootoca vivipara) in an extensivelly used farmland in Poland. Folia biol. (Kraków) 56: 165-171. The study was carried out in extensive farmland area near the town of Odolanów, Poland. During two breeding seasons (April-May, 2006-2007) lizards were counted on transect routes and captured by hand or by noosing. In total, 123 specimens of L. agilis and 153 specimens of Z. vivipara were captured. The proportion of males to females wasnt differed from the theoretical 1:1 ratio. Almost half of the individuals exhibited tail autotomy at least once in life. In the studied sand lizards significant sex specific differences were found between all morphological traits, i.e. males were shorter, lighter, but had a bigger head. In common lizards significant sex specific differences were detected only in body length, i.e. females were longer. All of the morphological traits were highly inter-correlated. Key words: Biometry, common lizard, sand lizard, co-occurrence, farmland, Poland. Anna EKNER, Marcin ANTCZAK, Maciej BOGACZYK, Piotr TRYJANOWSKI, Department of Be- havioural Ecology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznañ, Poland. E-mail: [email protected] Igor MAJLÁTH, Martin BONA, Institute of Biology and Ecology, P.
    [Show full text]
  • The Sand Lizard, Lacerta Ag/Lis, in Italy: Preliminary
    SHORT NOTES 101 REFERENCES HERPETOLOGICAL JOURNAL, Vol. 2, pp. 101-103 (1992) Anonymous ( 1987). The flying death ofRa jputana - systematics of dan­ THE SAND LIZARD, LACERTA AG/LIS, gerously venomous snakes. In Report of the British Museum (Natural History), 1984- 1985. London: British Museum (Natural IN ITALY: PRELIMINARY DATA ON History). DISTRIBUTION AND HABITAT Harding, K. A., & Welch, K. R. G. ( 1980). Venomous snakes of the CHARACTERISTICS World. A checklist. Oxford: Pergamon Press. MASSIMO CAPULA AND LUCA LUISELLI Leviton, A. E. ( 1980). Museum acronyms -second edition. Herpetologica/ Review 11, 93- 102. Dipartimento di Biologia Animate e del/ ' Uomo, Universitii "la Sapienza " di Roma, Via Borelli 50, 00161 Roma, Italy Looareesuwan, S., Viravan, C. & Warrell, D. A. ( 1988). Factors contrib­ uting to fatal snake bite in the tropics: analysis of 46 cases in (Accepted 30. 1.91) Thailand. Transactions of the Royal Societ.v o.lTropical Medicine and Hygiene 82, 930-934. Lacerta agilis Linnaeus is a lacertid lizard whose wide range extends from NE Iberia and W France to central Asia through Myint-Lwin, Phillips, R. E., Tun-Pe, Warrell, D. A., Tin-Nu-Swe & Maung-Maung-Lay. ( 1985). Bites by Russell's viper (Vipera most of Europe. This species is rare or absent fromthe European russelli siamensis) in Burma: Haemostatic, vascular, and renal regions characterized by a Mediterranean climate, such as most disturbances and response to treatment. The lancet 2, 1259- of the Iberian Peninsula, the Italian Peninsula and S Balkans 1264. (Arnold & Burton, 1978; Jablokov, Baranow & Rozanow, 1980; Bischoff, 1984, 1988). The occurrence of the sand lizard Thorpe, R.
    [Show full text]
  • Eurasian Lizards
    LACERTIDAE—LACERTINAE EURASIAN LIZARDS he subfamily Lacertinae was the large sister keel-scaled, long-tailed lizards are the only Ttaxon to the Gallotinae. It was divided into representatives of the Lacertinae throughout most two tribes, the Eurasian Lacertini and the Afro- of their range from Amur, Russia, and Japan, to Asian Eremiadini, but authors now seem to prefer Bangladesh and Indonesia. elevating these tribes to subfamily level. The Many of the European genera were formerly Lacertinae has a primarily Mediterranean subgenera of Lacerta, which is now reduced to only distribution that spreads eastward into the Middle ten species, including the highly variably patterned East and Central Asia, although there is one Far Sand Lizard (L. agilis), which, although rare and East Asian and Southeast Asian genus, Takydromus, localized in its distribution in the UK, is found the Oriental grass lizards. The 24 species of across a huge swathe of territory from Europe to LACERTINAE Scelarcis, Takydromus, Teira, Timon, DISTRIBUTION and Zootoca Europe, and southwestern, Central, HABITATS Southeast, and Far East Asia Heathland, sand dunes, grassland, maquis, GENERA riverbanks, rocky outcrops and ruins, Algyroides, Anatololacerta, Apathya, coastal islands, and rocky mountains Archaeolacerta, Dalmatolacerta, SIZE Darevskia, Dinarolacerta, Hellenolacerta, SVL 1¾ in (45 mm) Pygmy Keeled Lizard Iberolacerta, Iranolacerta, Lacerta, (Algyroides fitzingeri) to 10¼ in (260 mm) Parvilacerta, Phoenicolacerta, Podarcis, European Eyed Lizard (Timon lepidus) 154 LACERTOIDEA—Lacertids and teiids the Lake Baikal region of Central Asia. Although Among the most attractive species are the male the range of the Sand Lizard is impressive, it is green-bodied, blue-throated Western and Eastern eclipsed by that of the Viviparous Lizard (Zootoca Green Lizards (L.
    [Show full text]
  • Saryarka - Steppe and Lakes of Northern Kazakhstan Kazakhstan
    SARYARKA - STEPPE AND LAKES OF NORTHERN KAZAKHSTAN KAZAKHSTAN The area preserves two large lake systems in a diverse and barely altered dry central Eurasian steppe, an ecosystem much reduced since its reclamation for agriculture in the 1950s. Northern pine forest overlaps semi-arid desert flora at Naurzum; and the Korgalzhyn-Tengiz lakes, on a major migratory crossroads, support the largest breeding, moulting and resting waterbird populations in Asia, including relict, endemic and rare species. COUNTRY Kazakhstan NAME Saryarka - Steppe and Lakes of Northern Kazakhstan NATURAL WORLD HERITAGE SERIAL SITE 2008: Inscribed on the World Heritage List under natural criteria ix and x. STATEMENT OF OUTSTANDING UNIVERSAL VALUE The UNESCO World Heritage Committee issued the following Statement of Outstanding Universal Value at the time of inscription Values Saryarka - Steppe and Lakes of Northern Kazakhstan protects substantial, largely undisturbed areas of Central Asian steppe and lakes in the Korgalzhyn and Naurzum State Nature Reserves. The property’s wetland areas are of outstanding importance for migratory waterbirds, including substantial populations of globally threatened species, as they are key stopover points and crossroads on the Central Asian flyways. The property’s steppe areas provide a valuable refuge for over half the species of the region’s steppe flora, a number of threatened bird species and the critically endangered Saiga antelope. Criterion (ix): Ongoing biological and ecological processes: The property contains substantial areas of steppe and lakes with largely undisturbed associated biological and ecological processes. The seasonal dynamics of the hydrology, chemistry and biology of the lakes, with the diverse flora and fauna of the wetlands have evolved through complex wetting and drying cycles, and are of global significance and scientific interest.
    [Show full text]
  • An Assessment of the Impact of Conservation Grazing on Reptile Populations
    Amphibian and Reptile Conservation RESEARCH REPORT 12/01 An assessment of the impact of conservation grazing on reptile populations G.M. Jofré & C.J. Reading ACKNOWLEDGEMENTS We wish to thank the following people and organisations for help in providing data used in this review: Biological Records Centre (BRC), The Forestry Commission, D. Galliford from The Centre for Ecology & Hydrology (CEH) and Alison Turnock from the Purbeck District Council. SUGGESTED CITATION: Jofré1, G.M. & Reading2, C.J. (2012). An assessment of the impact of conservation grazing on reptile populations. ARC Research Report 12/01. 1: Amphibian and Reptile Ecologist E-mail: [email protected] 2: Centre for Ecology and Hydrology CEH Wallingford, Oxon. OX10 8BB. E-mail: [email protected] Amphibian and Reptile Conservation Research Reports publish the results of research and/or monitoring activities of interest to the herpetological community. Any views or opinions presented in this publication do not necessarily represent those of Amphibian and Reptile Conservation (ARC) or its collaborators. Whilst our goal is that the information herein is timely and accurate, ARC or its collaborators can accept no responsibility or liability with regards to that information. Reproduction and distribution (e.g. by photocopy or in pdf format) of this Report, acknowledging the source, is permitted for non-commercial purposes only. 2 CONTENTS Page Contents 3 List of Tables and Figures 5 Executive Summary 7 1. Introduction 9 2. The effects of grazing on habitats and their communities 10 2.1 Areas adjacent to fresh water 11 2.2 Grassland 11 2.3 Sand dunes 12 2.4 Lowland heathland 13 2.5 Use of habitat 14 2.6 Use of space 14 2.7 Diet 15 2.8 Differences of impact between ponies and cattle 15 3.
    [Show full text]
  • Adaptive Color Variation Along an Elevational Gradient. the Case of the Mediterranean Lizard Psammodromus Algirus. Universidad De Granada, Spain
    Adaptive color variation along an elevational gradient. The case of the Mediterranean lizard Psammodromus algirus. Variación adaptativa de la coloración en un gradiente altitudinal. El caso del lacértido mediterráneo Psammodromus algirus. TESIS DOCTORAL Senda Reguera Panizo, Granada, 2015 Departamento de Zoología Programa de doctorado: Biología Fundamental y de Sistemas Tesis impresa en Granada Diciembre de 2014 Como citar: Reguera S. 2015. Adaptive color variation along an elevational gradient. The case of the Mediterranean lizard Psammodromus algirus. Universidad de Granada, Spain. Foto de portada y contraportada: Senda Reguera Panizo La mayoría de las fotografías han sido tomadas por la autora de la tesis, pero algunas han sido cedidas por Laureano González y por Virve Sõber. Ilustraciones realizadas por Lina Krafel Retoque Figura 4.3: Antonio Aragón Rebollo Adaptive color variation along an elevational gradient. The case of the Mediterranean lizard Psammodromus algirus. Variación adaptativa de la coloración en un gradiente altitudinal. El caso del lacértido mediterráneo Psammodromus algirus. Memoria presentada por la Licenciada Senda Reguera Panizo para optar al Grado de Doctora en Biología por la Universidad de Granada. Tesis realizada bajo la dirección del Dr. Gregorio Moreno Rueda VB director Fdo: Dr. Gregorio Moreno Rueda La doctoranda Fdo: Lda. Senda Reguera Panizo Granada, 2014 El Dr. Gregorio Moreno Rueda, Profesor Ayudante Doctor de la Universidad de Granada CERTIFICAN: Que los trabajos de la investigación desarrollada en la Memoria de Tesis Doctoral: “Adaptive color variation along an elevational gradient. The case of the Mediterranean lizard Psammodromus algirus”, son aptos para ser presentados por la Lda. Senda Reguera Panizo ante el Tribunal que en su día se designe, para optar al Grado de Doctora por la Universidad de Granada.
    [Show full text]
  • Reptiles in Your Garden: Your Questions Answered
    Reptiles in your garden: your questions answered www.naturalengland.org.uk Legal protection Because of their rarity, all British reptiles Reptiles in your garden Reptiles are an important part of our are protected against killing, injury wildlife heritage and play a key role and sale. The smooth snake and sand Reptiles in England in natural ecosystems. Unfortunately, lizard receive additional protection all English reptiles have declined in that prohibits their disturbance or England is home to three species of snake (grass snake, adder and smooth numbers over the last few decades, capture and damage caused to their snake) and three species of lizard (common lizard, slow-worm and sand mainly because their habitats have habitat. There is a fine of up to £5,000 lizard). They are fascinating animals, but are often overlooked or been lost, fragmented or poorly and/or up to six months imprisonment misunderstood. Snakes, in particular, have suffered from a poor public image. managed. for any of these offences. In many cases, reptiles will go entirely unnoticed in a garden, but sometimes their discovery will provoke fear. This booklet should ease any worries you may Which reptiles live in my garden? have and provide answers to many common questions. Which snake? encountered snake species. Adders Grass snakes are adept swimmers, and hunt in garden ponds for amphibians. Try to take note of the snake’s rarely turn up in gardens, but if you Paul Sterry/Nature Photographers Ltd markings and colouration. The chart live close to particular habitats in on pages 12-13 and accompanying certain parts of the country you may pictures should help you to identify it.
    [Show full text]
  • Sand Lizard and Natterjack Toad Recovery Project 2011- 2014
    Sand Lizard and Natterjack Toad Recovery Project 2011- 2014 Moulton N and Buckley J Amphibian and Reptile Conservation Trust Report No 32 July 2015 Date www.naturalresourceswales.gov.uk About Natural Resources Wales Natural Resources Wales is the organisation responsible for the work carried out by the three former organisations, the Countryside Council for Wales, Environment Agency Wales and Forestry Commission Wales. It is also responsible for some functions previously undertaken by Welsh Government. Our purpose is to ensure that the natural resources of Wales are sustainably maintained, used and enhanced, now and in the future. We work for the communities of Wales to protect people and their homes as much as possible from environmental incidents like flooding and pollution. We provide opportunities for people to learn, use and benefit from Wales' natural resources. We work to support Wales' economy by enabling the sustainable use of natural resources to support jobs and enterprise. We help businesses and developers to understand and consider environmental limits when they make important decisions. We work to maintain and improve the quality of the environment for everyone and we work towards making the environment and our natural resources more resilient to climate change and other pressures. www.naturalresourceswales.gov.uk Page 1 Evidence at Natural Resources Wales Natural Resources Wales is an evidence based organisation. We seek to ensure that our strategy, decisions, operations and advice to Welsh Government and others are underpinned by sound and quality-assured evidence. We recognise that it is critically important to have a good understanding of our changing environment.
    [Show full text]
  • Reptile Survey in Dinara Mountain (Croatia) Revealed the Southernmost Known Population of Horvath’S Rock Lizard (Iberolacerta Horvathi)
    Nat. Croat. Vol. 23(1), 2014 235 NAT. CROAT. VOL. 23 No 1 235–240 ZAGREB June 30, 2014 short communication / kratko priopćenje REPTILE SURVEY IN DINARA MOUNTAIN (CROATIA) REVEALED THE SOUTHERNMOST KNOWN POPULATION OF HORVATH’S ROCK LIZARD (IBEROLACERTA HORVATHI) Anamarija Žagar*1,2, Miguel A. Carretero1, Miha Krofel2, Martina Lužnik3, Martina Podnar4 & Nikola Tvrtković5 1CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661 Vairão, Portugal 2Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia 3Faculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, Glagoljaška 8, 6000 Koper, Slovenia 4Croatian Natural History Museum, Demetrova 1, 10000 Zagreb, Croatia 5Natura, Society of Nature Protection, Croatia; home address: Alagovićeva 21, 10000 Zagreb, Croatia Žagar, A., Carretero, M. A., Krofel, M., Lužnik, M., Podnar, M. & Tvrtković, N.: Reptile survey in Dinara Mountain (Croatia) revealed the southernmost known population of Horvath’s rock li- zard (Iberolacerta horvathi). Nat. Croat., Vol. 23, No. 1, 235–240, 2014, Zagreb. The article presents results from a reptile survey conducted between 14 and 16 June 2013 in a mon- tane and subalpine belt of the western part of Dinara Mountain in Croatia. The most interesting result is the southernmost finding of Horvath’s Rock lizard Iberolacerta( horvathi). In the survey area we also recorded eight other reptile species: Anguis fragilis, Lacerta agilis, L. viridis sensu lato, Podarcis muralis, P. melissellensis, Coronella austriaca, Zamenis longissimus, and Vipera ammodytes. Keywords: reptiles, Horvath’s Rock lizard, Iberolacerta horvathi, Dinara Mt., Croatia Žagar, A., Carretero, M. A., Krofel, M., Lužnik, M., Podnar, M.
    [Show full text]
  • Lacerta Agilis Is One of Only Six Rep­ Tiles Native to Britain
    HERPETOLOGICAL JOURNAL, Vol. 11, pp. 23-27 (2001) A GENETIC ASSESSMENT OF BRITISH POPULATIONS OF THE SAND LIZARD (LA CERTA AGILIS) TREVOR J. C . BEEBEE A D GRAHAM ROWE School of Biological Sciences, University of Sussex, Falmer, Brighton BN I 9QG We investigated sand lizard (Lacer/a agilis) populations in Britain by genetic analysis across eight polymorphic microsatellite loci. Genetic diversity as determined by mean expected heterozygosity was high in all three distinct regions where the species occurs (Dorset, Surrey and Merseyside), though allelic diversity was lower on Merseyside than in Surrey or Dorset. There was significant genetic differentiation between populations in all three of these widely separated zones, as judged both by Fst and Rst estimators. A genetic test for population bottlenecks confirmed that in at least two of the areas currently inhabited, Surrey and Merseyside, L. agilis has undergone substantial recent declines. The significance of these findings for sand lizard conservation is discussed. Key words : sand lizards, Lacer/a, conservation, genetics, microsatellites INTRODUCTION in the three areas where the species currently exists in Britain. The sand lizard Lacerta agilis is one of only six rep­ tiles native to Britain. It is also one of the two rarest, MATERIALS AND METHODS confined within recent historical times to three small ar­ SAMPLING eas of suitable habitat (Smith, I 95 1 ): the lowland heaths of Dorset and south-west Hampshire, heathland Terminal digits from toes were obtained, under li­ in the Surrey Weald, and the coastal dunes of Mersey­ cence, from lizards in all three areas of the British side and North Wales.
    [Show full text]
  • Sand Lizard Surveys at Newborough Warren NNR and Sand Dune Habitat Management Guidance
    Sand lizard surveys at Newborough Warren NNR and sand dune habitat management guidance Peter Hill, Nick Moulton and Jim Foster Amphibian and Reptile Conservation Trust Report No 302 Date www.naturalresourceswales.gov.uk About Natural Resources Wales Natural Resources Wales’ purpose is to pursue sustainable management of natural resources. This means looking after air, land, water, wildlife, plants and soil to improve Wales’ well-being, and provide a better future for everyone. Evidence at Natural Resources Wales Natural Resources Wales is an evidence based organisation. We seek to ensure that our strategy, decisions, operations and advice to Welsh Government and others are underpinned by sound and quality-assured evidence. We recognise that it is critically important to have a good understanding of our changing environment. We will realise this vision by: • Maintaining and developing the technical specialist skills of our staff; • Securing our data and information; • Having a well resourced proactive programme of evidence work; • Continuing to review and add to our evidence to ensure it is fit for the challenges facing us; and • Communicating our evidence in an open and transparent way. This Evidence Report series serves as a record of work carried out or commissioned by Natural Resources Wales. It also helps us to share and promote use of our evidence by others and develop future collaborations. However, the views and recommendations presented in this report are not necessarily those of NRW and should, therefore, not be attributed to NRW. www.naturalresourceswales.gov.uk Page 1 Report series: NRW Evidence Report Report number: 302 Publication date: October 2018 Contract number: 8004055 Contractor: Amphibian and Reptile Conservation Trust Contract Manager: M.A.
    [Show full text]
  • Lacerta Agilis, Sand Lizard
    The IUCN Red List of Threatened Species™ ISSN 2307-8235 (online) IUCN 2008: T157288A5071439 Lacerta agilis, Sand Lizard Assessment by: Agasyan, A. et al. View on www.iucnredlist.org Short citation: Agasyan, A. et al. 2010. Lacerta agilis. The IUCN Red List of Threatened Species 2010: e.T157288A5071439. http://dx.doi.org/10.2305/IUCN.UK.2010-4.RLTS.T157288A5071439.en [see full citation at end] Copyright: © 2015 International Union for Conservation of Nature and Natural Resources Reproduction of this publication for educational or other non-commercial purposes is authorized without prior written permission from the copyright holder provided the source is fully acknowledged. Reproduction of this publication for resale, reposting or other commercial purposes is prohibited without prior written permission from the copyright holder. For further details see Terms of Use. The IUCN Red List of Threatened Species™ is produced and managed by the IUCN Global Species Programme, the IUCN Species Survival Commission (SSC) and The IUCN Red List Partnership. The IUCN Red List Partners are: BirdLife International; Botanic Gardens Conservation International; Conservation International; Microsoft; NatureServe; Royal Botanic Gardens, Kew; Sapienza University of Rome; Texas A&M University; Wildscreen; and Zoological Society of London. If you see any errors or have any questions or suggestions on what is shown in this document, please provide us with feedback so that we can correct or extend the information provided. THE IUCN RED LIST OF THREATENED SPECIES™ Taxonomy Kingdom Phylum Class Order Family Animalia Chordata Reptilia Squamata Lacertidae Taxon Name: Lacerta agilis Linnaeus, 1758 Synonym(s): • Lacerta boemica • Lacerta paradoxa • Lacerta stirpium • Seps argus • Seps caerulescens • Seps ruber Regional Assessments: • Europe Common Name(s): • English: Sand Lizard • French: Lezard des Souches • Spanish: Lagarto Ágil Taxonomic Notes: A phylogeographic study of this species based on mtDNA (Kalyabina et al.
    [Show full text]