Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 19 March 2018 doi:10.20944/preprints201803.0141.v1 Peer-reviewed version available at Genes 2018, 9, 225; doi:10.3390/genes9040225 Genome-guided analysis of the syntrophic acetate oxidizer C. ultunense and comparative genomics reveal different strategies for acetate oxidation and energy conservation in syntrophic acetate-oxidising bacteria Shahid Manzoor1, Anna Schnürer2, Erik Bongcam-Rudloff3, Bettina Müller2# 1Department of Information Technology, University of the Punjab, Lahore, Pakistan; 2Department of Microbiology, Swedish University of Agricultural Sciences, BioCenter, Uppsala, SE 750 07, Sweden; 3Department of Animal Breeding and Genetics Science, Swedish University of Agricultural Science, SLU-Global Bioinformatics Centre, Uppsala, SE 750 07, Sweden #Corresponding author
[email protected] © 2018 by the author(s). Distributed under a Creative Commons CC BY license. Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 19 March 2018 doi:10.20944/preprints201803.0141.v1 Peer-reviewed version available at Genes 2018, 9, 225; doi:10.3390/genes9040225 ABSTRACT Syntrophic acetate oxidation operates close to the thermodynamic equilibrium and very little is known about the participating organisms and their metabolism. Clostridium ultunense is one of the most abundant syntrophic acetate-oxidising bacteria (SAOB) found in engineered biogas processes operating with high ammonia concentrations. It has been proven to oxidise acetate in cooperation with hydrogenotrophic methanogens. There is evidence that the Wood- Ljungdahl (WL) pathway plays an important role in acetate oxidation. In this study we analysed the physiological and metabolic capacities of C. ultunense on genome scale and conducted a comparative study of all known characterised SAOB, namely Syntrophaceticus schinkii, Thermacetogenium phaeum, Tepidanaerobacter acetatoxydans and Pseudothermotoga lettingae.