Evolutionary and Functional Studies of the Mouse Retroviral Restriction Gene, Fvl
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Quaternary Murid Rodents of Timor Part I: New Material of Coryphomys Buehleri Schaub, 1937, and Description of a Second Species of the Genus
QUATERNARY MURID RODENTS OF TIMOR PART I: NEW MATERIAL OF CORYPHOMYS BUEHLERI SCHAUB, 1937, AND DESCRIPTION OF A SECOND SPECIES OF THE GENUS K. P. APLIN Australian National Wildlife Collection, CSIRO Division of Sustainable Ecosystems, Canberra and Division of Vertebrate Zoology (Mammalogy) American Museum of Natural History ([email protected]) K. M. HELGEN Department of Vertebrate Zoology National Museum of Natural History Smithsonian Institution, Washington and Division of Vertebrate Zoology (Mammalogy) American Museum of Natural History ([email protected]) BULLETIN OF THE AMERICAN MUSEUM OF NATURAL HISTORY Number 341, 80 pp., 21 figures, 4 tables Issued July 21, 2010 Copyright E American Museum of Natural History 2010 ISSN 0003-0090 CONTENTS Abstract.......................................................... 3 Introduction . ...................................................... 3 The environmental context ........................................... 5 Materialsandmethods.............................................. 7 Systematics....................................................... 11 Coryphomys Schaub, 1937 ........................................... 11 Coryphomys buehleri Schaub, 1937 . ................................... 12 Extended description of Coryphomys buehleri............................ 12 Coryphomys musseri, sp.nov.......................................... 25 Description.................................................... 26 Coryphomys, sp.indet.............................................. 34 Discussion . .................................................... -
New Issues in Genetic Counseling of Hereditary Colon Cancer Patrick M
New Issues in Genetic Counseling of Hereditary Colon Cancer Patrick M. Lynch Abstract Clinicians face significant challenges in the diagnosis and management of familial colorectal cancer predisposition. Many of the challenges concern the rarity of individual conditions and their unfamiliarity to most clinicians, even those in the subspecialty areas of gastroenterology, colorec- tal surgery, and medical oncology. Because the World Wide Web now offers a wealth of informa- tion, familiarity with available online resources should be a minimal expectation of clinicians. Notably, these same resources are available to the lay public, so a more informed group of patients can be expected and is already being encountered.The web sites noted throughout this article are merely early examples of what should become an opportunity for instant access to the most up-to-date knowledge of rare familial colorectal cancers and their clinical features, molecular diagnostics, and clinical management and prevention. Many professional organizations have pro- duced guidelines (in print and online) for use by practitioners in various specialties.The consisten- cy, growing evidence base, and ready availability of these guidelines to providers and patients alike will likely foster greater recognition of the need to be in compliance with them. Finally, as investigators make progress with the genetics of these rare diseases, one can anticipate a ‘‘coop- erative group’’approach to clinical trials. Identification of inherited susceptibility to colon cancer is now protein expression, which is followed by mutational testing readily possible, with genes identified for familial adenomatous when MSI and/or immunohistochemical screens are informa- polyposis (FAP) and its variants (1–3), hereditary nonpoly- tive. -
Phagemid-Based Method of Producing Filamentous Bacteriophage Particles Displaying Antibody Molecules and the Corresponding Bacteriophage Particles
Europäisches Patentamt *EP001433846A2* (19) European Patent Office Office européen des brevets (11) EP 1 433 846 A2 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: (51) Int Cl.7: C12N 15/10, C07K 16/00, 30.06.2004 Bulletin 2004/27 C12N 15/62, C12N 7/00, C12N 15/73 (21) Application number: 04005419.9 (22) Date of filing: 10.07.1991 (84) Designated Contracting States: • Griffiths, Andrew David AT BE CH DE DK ES FR GB GR IT LI LU NL SE Cambridge CB1 4AY (GB) • Jackson, Ronald Henry (30) Priority: 10.07.1990 GB 9015198 Cambridge CB1 2NU (GB) 19.10.1990 GB 9022845 • Holliger, Kaspar Philipp 12.11.1990 GB 9024503 Cambridge CB1 4HT (GB) 06.03.1991 GB 9104744 • Marks, James David 15.05.1991 GB 9110549 Kensington, CA 94707-1310 (US) • Clackson, Timothy Piers (62) Document number(s) of the earlier application(s) in Somerville, MA 02143 (US) accordance with Art. 76 EPC: • Chiswell, David John 97120149.6 / 0 844 306 Buckingham MK18 2LD (GB) 96112510.1 / 0 774 511 • Winter, Gregory Paul Cambridge CB2 1TQ (GB) (71) Applicants: • Bonnert, Timothy Peter • Cambridge Antibody Technology LTD Seattle, WA 98102 (US) Cambridge CB1 6GH (GB) • Medical Research Council (74) Representative: Walton, Seán Malcolm et al London W1B 1AL (GB) Mewburn Ellis LLP York House, (72) Inventors: 23 Kingsway • McCafferty, John London WC2B 6HP (GB) Babraham CB2 4AP (GB) • Pope, Anthony Richard Remarks: Cambridge CB1 2LW (GB) This application was filed on 08 - 03 - 2004 as a • Johnson, Kevin Stuart divisional application to the application mentioned Highfields, Cambridge CB3 7NY (GB) under INID code 62. -
Antibody Discovery for Development of a Serotyping Dengue Virus NS1 Capture Assay
Antibody Discovery for Development of a Serotyping Dengue Virus NS1 Capture Assay Kebaneilwe Lebani Master of Biotechnology (Advanced) A thesis submitted for the degree of Doctor of Philosophy at The University of Queensland in 2014 Australian Institute for Bioengineering and Nanotechnology ABSTRACT Dengue virus (DENV) infections are a significant public health burden in tropical and sub-tropical regions of the world. Infections are caused by four different but antigenically related viruses which result in four DENV serotypes. The multifaceted nature of DENV pathogenesis hinders the sensitivity of assays designed for the diagnosis of infection. Different markers can be optimally detected at different stages of infection. Of particular clinical importance is the identification of acute viremia during the febrile phase of infection which is pivotal for management of infection. Non-structural protein 1 (NS1) has been identified as a good early surrogate marker of infection with possible applications in epidemiological surveillance and the development of blood screening assays. This contribution is towards using serotype-specificity to achieve specific and more sensitive diagnostic detection of DENV NS1. The general aim of this work is to isolate immune-reagents that can be used to develop an assay with improved sensitivity of DENV NS1 detection in a diagnostic setting. In this work, we sought to isolate serotype-specific antibodies that discern discreet antigenic differences in NS1 from each DENV serotype. Additionally, we also sought to isolate a pairing antibody that recognises NS1 from all four DENV serotypes (pan-reactive) for tandem capture of the DENV NS1. To achieve this, three naive, immunoglobulin gene libraries (a VH domain, a scFv and a Fab library) were interrogated for binders to recombinant NS1 antigen from all four DENV serotypes using phage display technology and various biopanning approaches. -
The Search for the Elusive Link Between Genome Structure and Gene Function Michel Morange
Pseudoalleles and gene complexes: the search for the elusive link between genome structure and gene function Michel Morange To cite this version: Michel Morange. Pseudoalleles and gene complexes: the search for the elusive link between genome structure and gene function. Perspectives in Biology and Medicine, Johns Hopkins University Press, 2016, 58 (2), pp.196-204. 10.1353/pbm.2015.0027. hal-01347725 HAL Id: hal-01347725 https://hal.archives-ouvertes.fr/hal-01347725 Submitted on 21 Jul 2016 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives| 4.0 International License 1 Pseudoalleles and gene complexes: the search for the elusive link between genome structure and gene function Michel Morange, Centre Cavaillès, République des savoirs: Lettres, sciences, philosophie USR3608, Ecole normale supérieure, 29 rue d’Ulm, 75230 Paris Cedex 05, France E-mail: [email protected] ABSTRACT After their discovery in the first decades of the XXth century, pseudoalleles generated much interest among geneticists: they apparently violated the conception of the genome as a collection of independent genes elaborated by Thomas Morgan’s group. Their history is rich, complex, and deserves more than one short contribution. -
Molecular Genetics of Alzheimer's Disease
DkYb[_Y7Y_ZiWdZCeb[YkbWh8_ebe]o 23 I[h_[i;Z_jeh >$@$=heii Institut für Biochemie Biozentrum Am Hubland 97074 Würzburg Germany Dieter B. Wildenauer (Ed.) Molecular Biology of Neuropsychiatric Disorders With 15 Figures and 8 Tables Editor Dieter B. Wildenauer Graylands Hospital Center for Clinical Research in Neuropsychiatry (CCRN) Claremont WA 6910 Australia ISBN 978-3-540-85382-4 e-ISBN 978-3-540-85383-1 ISSN 0933-1891 Library of Congress Control Number: 2008933566 © 2009 Springer-Verlag Berlin Heidelberg This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer. Violations are liable to prosecution under the German Copyright Law. The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Cover design: Boekhorst Design BV, The Netherlands Printed on acid-free paper 9 8 7 6 5 4 3 2 1 springer.com Preface The intention of this book is to give an overview about ongoing research into molecular causes for disorders that affect the human brain. These disorders afflict mainly human behavior and are, since borders between “normal” and “abnormal” behaviors are continuous and hard to define, not always easy to diagnose. -
Evolutionary History of the Subgenus Mus in Eurasia with Special Emphasis on the House Mouse Mus Musculus
Papers in Honour of Ken Aplin edited by Julien Louys, Sue O’Connor and Kristofer M. Helgen Helgen, Kristofer M., Julien Louys, and Sue O’Connor. 2020. The lives of creatures obscure, misunderstood, and wonderful: a volume in honour of Ken Aplin 1958–2019 ..........................149 Armstrong, Kyle N., Ken Aplin, and Masaharu Motokawa. 2020. A new species of extinct False Vampire Bat (Megadermatidae: Macroderma) from the Kimberley Region of Western Australia ........................................................................................................... 161 Cramb, Jonathan, Scott A. Hocknull, and Gilbert J. Price. 2020. Fossil Uromys (Rodentia: Murinae) from central Queensland, with a description of a new Middle Pleistocene species ............................................................................................................. 175 Price, Gilbert J., Jonathan Cramb, Julien Louys, Kenny J. Travouillon, Eleanor M. A. Pease, Yue-xing Feng, Jian-xin Zhao, and Douglas Irvin. 2020. Late Quaternary fossil vertebrates of the Broken River karst area, northern Queensland, Australia ........................ 193 Theden-Ringl, Fenja, Geoffrey S. Hope, Kathleen P. Hislop, and Benedict J. Keaney. 2020. Characterizing environmental change and species’ histories from stratified faunal records in southeastern Australia: a regional review and a case study for the early to middle Holocene ........................................................................................... 207 Brockwell, Sally, and Ken Aplin. 2020. Fauna on -
Molecular Cloning and Functional Expression of Gibberellin 2- Oxidases, Multifunctional Enzymes Involved in Gibberellin Deactivation
Proc. Natl. Acad. Sci. USA Vol. 96, pp. 4698–4703, April 1999 Plant Biology Molecular cloning and functional expression of gibberellin 2- oxidases, multifunctional enzymes involved in gibberellin deactivation STEPHEN G. THOMAS,ANDREW L. PHILLIPS, AND PETER HEDDEN* Institute of Arable Crops Research (IACR)-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol BS41 9AF, United Kingdom Communicated by Jake MacMillan FRS, University of Bristol, Bristol, United Kingdom, February 16, 1999 (received for review December 22, 1998) ABSTRACT A major catabolic pathway for the gibberel- centration of bioactive GAs, the genes for these enzymes have lins (GAs) is initiated by 2b-hydroxylation, a reaction cata- not yet been isolated and it has not been possible to study their lyzed by 2-oxoglutarate-dependent dioxygenases. To isolate a regulation. GA 2b-hydroxylase cDNA clone we used functional screening Gibberellin 2b-hydroxylase activity is abundant in seeds of a cDNA library from developing cotyledons of runner bean during the later stages of maturation, particularly in legume (Phaseolus coccineus L.) with a highly sensitive tritium-release seeds, which accumulate large amounts of 2b-hydroxylated b assay for enzyme activity. The encoded protein, obtained by GAs (6–8). Indeed, GA8, the first 2 -hydroxyGA to be heterologous expression in Escherichia coli, converted GA9 to identified, was extracted from seeds of runner bean (Phaseolus b GA51 (2 -hydroxyGA9) and GA51-catabolite, the latter pro- coccineus, originally classified as P. multiflorus) (9). In certain duced from GA51 by further oxidation at C-2. The enzyme thus species, including legumes, further metabolism of 2b- is multifunctional and is best described as a GA 2-oxidase. -
Evolutionary Characterization of a Y Chromosomal Sequence Conserved in the Genus Mus
Genet. Res., Camb. (1988), 52, pp. 145-150 With 5 text-figures Printed in Great Britain 145 Evolutionary characterization of a Y chromosomal sequence conserved in the genus Mus YUTAKA NISHIOKA Department of Biology, McGill University, Montreal, Quebec, Canada H3A IBI (Received 8 October 1987 and in revised form 26 January 1988) Summary The extent of accumulation of mouse Y chromosomal repetitive sequences generally correlates with the known phylogenetic relationships in the genus Mus. However, we describe here a M. musculus Y chromosomal repetitive sequence, designated as ACClfl, whose accumulation patterns among eight Mus species do not correspond to their phylogenetic relationships. Although male-specific hybridization bands were present in all the species examined, significant accumulation (^ 200 copies) in the Y chromosomes was found in M. minutoides (subgenus Nannomys), M. pahari (subgenus Coelomys) and M. saxicola (subgenus Pyromys) as well as in the three closely related species M. hortulanus, M. musculus and M. spretus that belong to the subgenus Mus. Unexpectedly, the Y chromosomes of M. caroli and M. cookii (both subgenus Mus) had considerably reduced amounts of ACClfl-related sequences. Furthermore, in rats {Rattus norvegicus) the major accumulation sites appear to be autosomal. These observations suggest that caution must be taken in the interpretation of data obtained with repetitive sequences that have evolved quickly. 1. Introduction over 50 mouse (M. musculus) Y chromosomal sequences (Nishioka & Lamothe, 1987a). To date, 32 Recently several groups have isolated mouse DNA fragments were generated from 11 original (Mus musculus) Y chromosomal repetitive sequences isolates and their conservation in the genus Mus has (Bishop et al. -
Suggested Titles: April, 2014
Suggested Titles: April, 2014 For Library Collection Development (arranged alphabetically by subject) 1 Suggested Titles List April, 2014 Table of Contents Accounting .............................................................................................................................................................................. 2 Art ........................................................................................................................................................................................... 5 Biology ................................................................................................................................................................................... 15 Business Administration ....................................................................................................................................................... 26 Child Study ............................................................................................................................................................................ 31 Computer Information Science & Mathematics ................................................................................................................... 41 Criminal Justice ..................................................................................................................................................................... 52 Economics ............................................................................................................................................................................ -
3 Main Classification of Vectors (With Diagram)
4/20/2020 3 Main Classification of Vectors (With Diagram) Privacy Policy | Join our Community :- Upload Now 3 Main Classification of Vectors (With Diagram) Article shared by : The classifications are: 1. On the Basis of Our Aim with Gene of Interest 2. On the Basis of Host Cell Used 3. On the Basis of Cellular Nature of Host Cell. Vector Classification # 1. On the Basis of Our Aim with Gene of Interest: The point is what we are targeting from our gene of interest — its multiple copies or its protein product. Depending on these criteria vec tors are of following two types: 1. Cloning Vectors: We use a cloning vec tor when our aim is to just obtain numer ous copies (clones) of our gene of interest (hence the name cloning vectors). These are mostly used in construction of gene libraries. A number of organisms can be used as sources for cloning vectors. Some are created synthetically, as in the case of yeast artificial chromosomes and bacte rial artificial chromosomes, while others are taken from bacteria and bacteriopha ges. In all cases, the vector needs to be genetically modified in order to accommo date the foreign DNA by creating an in sertion site where the new DNA will fit ted. Example: PUC cloning vectors, pBR322 cloning vectors, etc. 2. Expression Vectors or Expression construct: www.biotechnologynotes.com/recombinant-dna-technology/3-main-classification-of-vectors-with-diagram/395 1/64 4/20/2020 3 Main Classification of Vectors (With Diagram) We use an expression vector when our aim is to obtain the protein prod uct of our gene of interest. -
Nobel Laureates in Physiology Or Medicine
All Nobel Laureates in Physiology or Medicine 1901 Emil A. von Behring Germany ”for his work on serum therapy, especially its application against diphtheria, by which he has opened a new road in the domain of medical science and thereby placed in the hands of the physician a victorious weapon against illness and deaths” 1902 Sir Ronald Ross Great Britain ”for his work on malaria, by which he has shown how it enters the organism and thereby has laid the foundation for successful research on this disease and methods of combating it” 1903 Niels R. Finsen Denmark ”in recognition of his contribution to the treatment of diseases, especially lupus vulgaris, with concentrated light radiation, whereby he has opened a new avenue for medical science” 1904 Ivan P. Pavlov Russia ”in recognition of his work on the physiology of digestion, through which knowledge on vital aspects of the subject has been transformed and enlarged” 1905 Robert Koch Germany ”for his investigations and discoveries in relation to tuberculosis” 1906 Camillo Golgi Italy "in recognition of their work on the structure of the nervous system" Santiago Ramon y Cajal Spain 1907 Charles L. A. Laveran France "in recognition of his work on the role played by protozoa in causing diseases" 1908 Paul Ehrlich Germany "in recognition of their work on immunity" Elie Metchniko France 1909 Emil Theodor Kocher Switzerland "for his work on the physiology, pathology and surgery of the thyroid gland" 1910 Albrecht Kossel Germany "in recognition of the contributions to our knowledge of cell chemistry made through his work on proteins, including the nucleic substances" 1911 Allvar Gullstrand Sweden "for his work on the dioptrics of the eye" 1912 Alexis Carrel France "in recognition of his work on vascular suture and the transplantation of blood vessels and organs" 1913 Charles R.