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Prism User's Guide 10 Copyright (C) 1999 Graphpad Software Inc User's Guide Version 3 The fast, organized way to analyze and graph scientific data ã 1994-1999, GraphPad Software, Inc. All rights reserved. GraphPad Prism, Prism and InStat are registered trademarks of GraphPad When you opened the disk envelope, or downloaded the Software, Inc. GraphPad is a trademark of GraphPad Software, Inc. purchased software, you agreed to the Software License Agreement reprinted on page 197. GraphPad Software, Inc. Use of the software is subject to the restrictions contained in the does not guarantee that the program is error-free, and cannot accompanying software license agreement. be held liable for any damages or inconvenience caused by er- rors. How to reach GraphPad Software, Inc: Although we have tested Prism carefully, the possibility of software errors exists in any complex computer program. You Phone: 858-457-3909 (619-457-3909 before June 12, 1999) should check important results carefully before drawing con- Fax: 858-457-8141 (619-457-8141 before June 12, 1999) clusions. GraphPad Prism is designed for research purposes Email: [email protected] or [email protected] only, and should not be used for the diagnosis or treatment of Web: www.graphpad.com patients. Mail: GraphPad Software, Inc. If you find that Prism does not fit your needs, you may return it 5755 Oberlin Drive #110 for a full refund (less shipping fees) within 90 days. You do not San Diego, CA 92121 USA need to contact us first. Simply ship the package to us with a note explaining why you are returning the program. Include your phone and fax numbers, and a copy of the invoice. If you purchased Prism through a dealer or catalog, please con- tact the company you purchased it from regarding returns. Analyzing repeated experiments ..................................................................... 62 Saving templates and methods ........................................................................ 64 Contents Analysis options ............................................................................................ 66 Graph types................................................................................................67 Automatic graphs........................................................................................... 67 XY graphs...................................................................................................... 68 Column graphs .............................................................................................. 73 Bar graphs with two grouping variables ........................................................... 74 Contents.......................................................................................................5 Bar graphs with one grouping variable ............................................................ 76 Changing the graph type ................................................................................ 79 Orientation ..................................................................................................9 Introducing GraphPad Prism.............................................................................9 Creating graphs ..........................................................................................81 System requirements ........................................................................................9 Making a new graph ...................................................................................... 81 Updating from a previous version ................................................................... 10 Graph options ............................................................................................... 81 How Prism is organized ................................................................................. 10 Create a series of similar graphs ...................................................................... 82 Starting GraphPad Prism................................................................................. 11 Choosing data to graph .................................................................................. 83 Hints for people who don't read manuals ........................................................ 13 The Change button ........................................................................................ 86 Tutorial ......................................................................................................15 Axes ...........................................................................................................87 Step 1. Start Prism.......................................................................................... 15 Overview of axes........................................................................................... 87 Step 2. Import data......................................................................................... 17 Formatting axes ............................................................................................. 87 Step 3. Make a graph ..................................................................................... 18 Custom ticks.................................................................................................. 91 Step 4. Fit a curve .......................................................................................... 20 Logarithmic axes............................................................................................ 94 Step 5. Create a second graph......................................................................... 22 Discontinuous axes........................................................................................ 98 Step 6. Arrange two graphs on a page layout.................................................... 24 Hiding axes and showing scale bars .............................................................. 101 Step 7. Embed a table..................................................................................... 26 Graphs with two Y-axes................................................................................ 102 Step 8. Exclude a value and update the graph .................................................. 27 Thickness and color of axes .......................................................................... 104 Step 9. Copy and paste to PowerPoint ............................................................. 28 Axis titles and numbering ............................................................................. 104 Entering and editing data ............................................................................29 Changing bars and symbols.......................................................................107 Overview of data tables.................................................................................. 29 Format symbols and lines ............................................................................. 107 The Welcome dialog...................................................................................... 30 Format bars ................................................................................................. 109 Column formats............................................................................................. 31 Format Columns dialog ................................................................................ 113 Creating additional data tables ........................................................................ 34 Legends ...................................................................................................... 116 Working with the data table............................................................................ 35 Error bars .................................................................................................117 Using the clipboard ....................................................................................... 37 Overview.................................................................................................... 117 Entering multiple sets of data that don’t share X values ..................................... 38 Displaying variability directly ....................................................................... 117 Embedding data tables in graphs or layouts...................................................... 39 Statistical theory: SD, SEM and 95% CI.......................................................... 119 Importing and pasting data .........................................................................41 Error bars on XY graphs and bar graphs ......................................................... 121 Overview of importing and pasting ................................................................. 41 Error bars on column bar graphs ................................................................... 123 Using data from Excel .................................................................................... 41 Page layouts .............................................................................................127 Using data from other spreadsheet or statistics programs................................... 45 An overview of page layouts......................................................................... 127 The Format Data Object dialog ....................................................................... 46 Changing the number or arrangement of graphs ............................................. 127 Data objects in Prism ..................................................................................... 52 Assigning graphs
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