United States Patent (19) 11 Patent Number: 5,939.426 Mccullough (45) Date of Patent: Aug

Total Page:16

File Type:pdf, Size:1020Kb

United States Patent (19) 11 Patent Number: 5,939.426 Mccullough (45) Date of Patent: Aug US00593.9426A United States Patent (19) 11 Patent Number: 5,939.426 McCullough (45) Date of Patent: Aug. 17, 1999 54 METHODS FORTREATING URINARY Ebert, W., 1977, “Soft Elastic Gelatin Capsules: A Unique INCONTINENCE USING Dosage Form”, Pharmaceutical Technology 1:44-50. DESCARBOETHOXYLORATADINE Goodman and Gilman's, 1996, The Pharmacological Basis of Therapeutics, 9th Ed. pp. 148, 588-592. 75 Inventor: John R. McCullough, Worcester, Mass. Herzog, A.R. et al., 1989, “Urinary Incontinence: Medical and Psychosocial Aspects”, Annu. Rev. Gerontol. Geriatr. 73 Assignee: Sepracor Inc., Marlborough, Mass. 9:74-119. Hilbert, J. et al., 1987, "Pharmacokinetics and Dose Pro 21 Appl. No.: 08/808,116 portionality of Loratadine”, J. Clin. Pharmacol. 27:694-698. 22 Filed: Feb. 28, 1997 Knowles, S., 1992, "Astemizole and Terfenadine-Induced Cardiovascular Effects”, Canadian J. Hospital Pharmacy (51) Int. Cl." ..................................................... A61K 31/44 45:33-34. 52 U.S. Cl. .............................................................. 514/290 Kohl and MacDonald, 1991, “New Pharmacologic 58 Field of Search ............................................... 514/290 Approaches to the Prevention of Space/Motion Sickness”, J. Clin. Pharmacol. 31:934-946. 56) References Cited Kubo, N. et al., 1987, "Antimuscarinic Effects of Antihis U.S. PATENT DOCUMENTS tamines: Quantitative Evaluation by Receptor-Binding Assay”, Japan J. Pharmacol. 43:277-282. 3,536.809 10/1970 Applezweig. 3,598,123 8/1971 Zaffaroni. Lathers, C. et al., 1989, “Pharmacology in Space', TiPS, 3,845,770 11/1974 Theeuwes et al.. 10:243-250. 3,916,899 11/1975 Theeuwes et al.. Levin and Wein, 1982, “Direct Measurement of the Anti 3,940,485 2/1976 Levinson et al. ....................... 424/250 cholinergic Activity of a Series of Pharmacological Com 4,008,796 2/1977 Aylon. pounds on the Canine and Rabbit Urinary Bladder”, J. 4,282,233 8/1981 Vilani. Urology 128:396-398. 4,659,716 4/1987 Villani et al.. 4,777,170 10/1988 Heinrich ............................... 514/226.2 Lunde, I., 1990, “Antihistamines”, Side Effects of Drugs 5,595.997 1/1997 Aberg et al. ............................ 514/290 14:135-138. Massad, C. et al., 1992, “The Pharmokinetics of Intravesical FOREIGN PATENT DOCUMENTS and Oral Oxybutynin Chloride”, J. Urology 148:595-597. WO 92/20377 11/1992 WIPO. McCue, J., 1996, “Safety of Antihistamines in the Treatment WO 96/20708 7/1996 WIPO. of Allergic Rhinitis in Elderly Patients”, Arch. Fam. Med. 5:464–468. OTHER PUBLICATIONS Mirakhur and Dundee, 1983, “Glycopyrrolate: Pharmacol Andersen and Feingold, 1995, “Adverse Drug Interactions ogy and Clinical Use', Anaesthesia 38:1195-1204. Clinically Important for the Dermatologist', Arch. Derma Mitchelson, F., 1992, “Pharmacological Agents Affecting tol. 131:468-473. Emesis', Drugs 43:443-463. Berge, S. et al., 1977, “Pharmaceutical Salts”, J. of Phar Muskat, Y. et al., 1996, “The Use of Scopolamine in the maceutical Sciences 66:1-19. Treatment of Detrusor Instability”, J. Urology Brandes, L. et al., 1992, “Stimulation of Malignant Growth 1.56:1989-1990. in Rodents by Antidepressant Drugs at Clinically Relevant Neiemans, F.A., 1988, “Antiallergic and Antitussive Drugs”, Doses', Cancer Research 52:3796-3800. Side Effects of Drugs 12:144-147. Brandes, L. et al., 1994, "Enhanced Cancer Growth in Mice Peggs and Shimp, 1995, “Antihistamines: The Old and The Administered Daily Human-Equivalent Doses of Some New', American Family Physician 52:593-600. H-Antihistamines: Predictive In Vitro Correlates”, J. Natl. Quercia and Broisman, 1993, "Focus on Loratadine: A New Cancer Institute 86:770–775. Second-Generation Nonsedating H-Receptor Antagonist”, Carmeliet, 1992, “Voltage- and Time-Dependent Block of Hosp. Formul. 28:137-153. the Delayed K Current in Cardiac Myocytes by Dofetilide', Resnick, N., 1995, “Urinary Incontinence”, The Lancet J. Pharmocol. Exper. Ther. 262:809–817. 346:94-99. Cheung, B.S.K., et al., 1992, “Investigation of Anti-Motion Roman and Danzig, 1993, “Loratadine”, Clinical Reviews in Sickness Drugs in the Squirrel Monkey”, J. Clin. Pharmacol. Allergy 11:89-110. 32:163-175. Clissold et al., 1989, “Loratadine: A Preliminary Reivew of (List continued on next page.) its Pharmacodynamic Properties and Therapeutic Efficacy”, Drugs 37:42–57. Primary Examiner Minna Moezie Cohen, B. et al., 1972, “Meclizine and Placebo in Treating Attorney, Agent, or Firm-Pennie & Edmonds LLP Vertigo of Vestibular Origin”, Arch. Neurol. 27:129-137. 57 ABSTRACT Cooke, R.D., 1983, “Glycopyrrolate in Bladder Dysfunc tion”, S. Afr. Med. J. 63:3. Methods for treating urinary incontinence comprising Craft, T., 1986, "Torsade de Pointes after Astemizole Over administering a therapeutically effective amount of dose”, British Medical Journal 292:660. deScarboethoxyloratadine, or a pharmaceutically acceptable Dörje, F. et al., 1991, “Antagonist Binding Profiles of Five Salt thereof. Cloned Human Muscarinic Receptor Subtypes”, J. Pharma cology and Experimental Therapeutics 256:727-733. 7 Claims, 1 Drawing Sheet 5,939.426 Page 2 OTHER PUBLICATIONS Yarker, Y. et al., 1995, “Oxybutynin', Drugs & Aging 6:243-262. Simons, F.E.R. et al., 1988, “Astemizole-Induced Torsade de Pointes', The Lancet 2:624. M.N.G. Dukes et al., “Side Effects of Drugs Annual 12.” Simons, F.E.R., 1994, “H-Receptor Antagonists”, Drug Elsevier Science Publishers B.V.: 142-143 (1988). Safety 10:350–380. Petrin, A., 1974, "Motion Sickness and its Treatment', Wein, A., 1995, “Pharmacology of Incontinence', Urol. Schweiz. Med. 63:79-81. Clin. N. Am. 22:557-577. Wood, C.D. et al., 1987, "Mechanisms of Antimotion Sick Wood, C., 1979, “Antimotion Sickness and Antiemetic ness Drugs”, Aviation, Space, and Envir. Med. 58 (9, Suppl.) Drugs", Drugs 17:471-479. pp. A262-A265. U.S. Patent Aug. 17, 1999 5,939.426 K RABBT VENTRICULAR CELL (%) (7) (7) E = -50 V 1 OO - -, (7) H 80 period 30s 60 40 Xs = 8.818 EM-6 20 COeff. = 1.836 O ------ 10-7 10-6 10-5 10-4 10-3 conc. loratadine (M) FIG. RABBT VENTRICULAR CELL EH= -50 mV (11) period 30S -6 20 - COeff. = 1.032 O - ---G- 10-7 10-6 10-5 10-4 10-3 Conc. descarboethoxyloratadine (M) FIG. 2 5,939.426 1 2 METHODS FOR TREATING URINARY Muskat et al. State that when the drug is administered by an INCONTINENCE USING oral or Systemic route, it causes Severe side-effects and also DESCARBOETHOXYLORATADNE discuss the contradictory reports regarding the transdermal administration of Scopolamine. Muskat et al. report that FIELD OF THE INVENTION although Scopolamine is known to cause cycloplegia and dryneSS of the mouth, the Side-effects in its Study were not The present invention relates to methods for treating So Severe to require discontinuation of the medication (Id. at urinary incontinence, Vertigo and motion Sickness. 1990). Scopolamine is also reported as being used widely for motion SickneSS and as effective for the treatment of Vertigo BACKGROUND OF THE INVENTION (Id.). However, it has also been reported that Scopolamine Urinary incontinence, Such as incontinence caused by has unwanted sedative effects (see Lathers et al., TIPS bladder detrusor muscle instability, is a prevalent problem 10:243–250 (1989)). that affects people of all ages and levels of physical health, Certain first generation H-receptor antihistamines, Such both in healthcare Settings and in the community at large. At as diphenylpyraline and promethazine, have been reported present, urinary incontinence afflicts 15-30% of elderly as having Significant affinity for the muscarinic receptors people living at home, one-third of those living in acute-care 15 (Kubo et al., Japan J. Pharmacol. 43:277–282 (1987)). Settings, and at least one-half of those in long-term care Some first generation H receptor antihistamines, Such as institutions (Resnick, R. M., Lancet 346:94 (1995)). dimenhydrinate, cyclizine and meclizine, have also been Medically, it predisposes perSons to urinary tract infections, found to be effective for treating either vertigo or motion pressure ulcers, perineal rashes, and uro Sepsis. sickness (Id; Wood, C., Drugs 17:471-479 (1979); Cohen et PsychoSocially, urinary incontinence is associated with al., Archives of Neurology 27: 129-135 (1972); see also embarrassment, Social Stigmatization, depression, and with Goodman & Gilman's, The Pharmacological Basis of the risk of institutionalization (Herzo et al., Annu. Rev. Therapeutics, 9th Ed. p. 588, 592 (1996)). Gerontol. Geriatr. 9:74 (1989)). Economically, the costs are Peggs et al. (American Family Physician 52(2):593-600 great; in the United States alone, over S15 billion is spent per (1995)), note that classic antihistamines, which have a annum managing incontinence. 25 greater propensity to croSS the blood-brain barrier, would Treatments for incontinence include drugs with bladder appear to be better indicated for the treatment of these relaxant properties, i.e., which help to control bladder detru conditions. However, the first generation H antihistamines sor muscle overactivity. Such drugs are effective in 80 to have undesirable side-effects, Such as Sedation (see Good 85% of patients with uninhibited bladder contractions. Anti mans & Gilman's, The Pharmacological Basis of cholinergic medications represent the mainstay of this type Therapeutics, 9th Ed. p. 590 (1996). of treatment. The major proportion of the neurohumoral The Second generation H-receptor antihistamines, Such Stimulus for physiologic bladder contraction is as terfenadine, astemizole and loratadine, while having acetylcholine-induced Stimulation of post ganglionic mus fewer Sedative effects, are reported as having weak or no carinic receptor Sites on bladder Smooth muscle. For effect on muscarinic receptors (Goodman & Gilman's, The example, anticholinergicS Such as propantheline bromide 35 Pharmacological Basis of Therapeutics, 9th Ed. p. 588 and glycopyrrolate, and combination Smooth muscle (1996); Simons, F. E., Drugs Safety 10(5):350–380 (1994)). relaxant/anticholinergicS Such as racemic oxybutynin and This is consistent with the findings that Such compounds do dicyclomine, have been used to treat urge incontinence. not possess any significant anticholinergic affects (see (See, e.g., Wein, A. J., Urol.
Recommended publications
  • Submaxillary Gland and the A2a- and A2b-Adrenoceptor Subtypes 1Anton D
    Br. J. Pharmacol. (1989), 98, 890-897 Differences between the cx2-adrenoceptor in rat submaxillary gland and the a2A- and a2B-adrenoceptor subtypes 1Anton D. Michel, Dana N. Lodry & Roger L. Whiting Institute of Pharmacology, Syntex Research, 3401 Hillview Avenue, Palo Alto, CA, 94303, U.S.A. 1 The a2-adrenoceptors of rat submaxillary gland, labelled with [3H]-rauwolscine, were character- ized by use of a range of subtype selective ligands and were compared to rabbit spleen a2A-adrenoceptors and rat kidney a2B-adrenoceptors. 2 In rat submaxillary gland, [3H]-rauwolscine labelled an apparently homogeneous population of binding sites with relatively low affinity (Kd= 11.65 nM) compared to the affinity in rat kidney (Kd = 2.18 nM) and rabbit spleen (Kd = 4.64 nM). 3 In competition studies using 16 ligands the a2-adrenoceptors in rat submaxillary gland appeared to differ from both the x2A-adrenoceptor of rabbit spleen (r = 0.62) and also the a2B-adrenoceptor of rat kidney (r = 0.28). 4 The affinity data obtained with benoxathian, imiloxan and WB 4101 indicated the presence of an a2B-adrenoceptor in rat submaxillary gland. However, data for chlorpromazine, oxymetazoline, spiroxatrine and xylometazoline indicated that submaxillary gland a2-adrenoceptors were of the a2A subtype. The affinity estimate for prazosin in rat submaxillary gland was intermediate between its affinity at the ae2A- and a2B-adrenoceptors while affinity estimates for idazoxan and phentolamine in rat submaxillary gland were greater than those obtained at either the c2A- or x2B-adrenoceptor. 5 These data indicate that rat submaxillary gland a2-adrenoceptors differ from the CX2A- and a2B-adrenoceptors found in rabbit spleen and rat kidney, respectively.
    [Show full text]
  • The Role of Acetylcholine in Cocaine Addiction
    Neuropsychopharmacology (2008) 33, 1779–1797 & 2008 Nature Publishing Group All rights reserved 0893-133X/08 $30.00 www.neuropsychopharmacology.org Perspective The Role of Acetylcholine in Cocaine Addiction ,1 1,2 Mark J Williams* and Bryon Adinoff 1Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, TX, USA; 2Mental Health Service, VA North Texas Health Care System, Dallas, TX, USA Central nervous system cholinergic neurons arise from several discrete sources, project to multiple brain regions, and exert specific effects on reward, learning, and memory. These processes are critical for the development and persistence of addictive disorders. Although other neurotransmitters, including dopamine, glutamate, and serotonin, have been the primary focus of drug research to date, a growing preclinical literature reveals a critical role of acetylcholine (ACh) in the experience and progression of drug use. This review will present and integrate the findings regarding the role of ACh in drug dependence, with a primary focus on cocaine and the muscarinic ACh system. Mesostriatal ACh appears to mediate reinforcement through its effect on reward, satiation, and aversion, and chronic cocaine administration produces neuroadaptive changes in the striatum. ACh is further involved in the acquisition of conditional associations that underlie cocaine self-administration and context-dependent sensitization, the acquisition of associations in conditioned learning, and drug procurement through its effects on arousal and attention. Long-term cocaine use may induce neuronal alterations in the brain that affect the ACh system and impair executive function, possibly contributing to the disruptions in decision making that characterize this population. These primarily preclinical studies suggest that ACh exerts a myriad of effects on the addictive process and that persistent changes to the ACh system following chronic drug use may exacerbate the risk of relapse during recovery.
    [Show full text]
  • Viewed the Existence of Multiple Muscarinic CNS Penetration May Occur When the Blood-Brain Barrier Receptors in the Mammalian Myocardium and Have Is Compromised
    BMC Pharmacology BioMed Central Research article Open Access In vivo antimuscarinic actions of the third generation antihistaminergic agent, desloratadine G Howell III†1, L West†1, C Jenkins2, B Lineberry1, D Yokum1 and R Rockhold*1 Address: 1Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, MS 39216, USA and 2Tougaloo College, Tougaloo, MS, USA Email: G Howell - [email protected]; L West - [email protected]; C Jenkins - [email protected]; B Lineberry - [email protected]; D Yokum - [email protected]; R Rockhold* - [email protected] * Corresponding author †Equal contributors Published: 18 August 2005 Received: 06 October 2004 Accepted: 18 August 2005 BMC Pharmacology 2005, 5:13 doi:10.1186/1471-2210-5-13 This article is available from: http://www.biomedcentral.com/1471-2210/5/13 © 2005 Howell et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Muscarinic receptor mediated adverse effects, such as sedation and xerostomia, significantly hinder the therapeutic usefulness of first generation antihistamines. Therefore, second and third generation antihistamines which effectively antagonize the H1 receptor without significant affinity for muscarinic receptors have been developed. However, both in vitro and in vivo experimentation indicates that the third generation antihistamine, desloratadine, antagonizes muscarinic receptors. To fully examine the in vivo antimuscarinic efficacy of desloratadine, two murine and two rat models were utilized. The murine models sought to determine the efficacy of desloratadine to antagonize muscarinic agonist induced salivation, lacrimation, and tremor.
    [Show full text]
  • Acetylcholine Signaling System in Progression of Lung Cancers
    Pharmacology & Therapeutics 194 (2019) 222–254 Contents lists available at ScienceDirect Pharmacology & Therapeutics journal homepage: www.elsevier.com/locate/pharmthera Acetylcholine signaling system in progression of lung cancers Jamie R. Friedman a,1, Stephen D. Richbart a,1,JustinC.Merritta,KathleenC.Browna, Nicholas A. Nolan a, Austin T. Akers a, Jamie K. Lau b, Zachary R. Robateau a, Sarah L. Miles a,PiyaliDasguptaa,⁎ a Department of Biomedical Sciences, Joan C. Edwards School of Medicine, 1700 Third Avenue, Huntington, WV 25755 b Biology Department, Center for the Sciences, Box 6931, Radford University, Radford, Virginia 24142 article info abstract Available online 3 October 2018 The neurotransmitter acetylcholine (ACh) acts as an autocrine growth factor for human lung cancer. Several lines of evidence show that lung cancer cells express all of the proteins required for the uptake of choline (choline Keywords: transporter 1, choline transporter-like proteins) synthesis of ACh (choline acetyltransferase, carnitine acetyl- Lung cancer transferase), transport of ACh (vesicular acetylcholine transport, OCTs, OCTNs) and degradation of ACh (acetyl- Acetylcholine cholinesterase, butyrylcholinesterase). The released ACh binds back to nicotinic (nAChRs) and muscarinic Cholinergic receptors on lung cancer cells to accelerate their proliferation, migration and invasion. Out of all components Proliferation of the cholinergic pathway, the nAChR-signaling has been studied the most intensely. The reason for this trend Invasion Anti-cancer drugs is due to genome-wide data studies showing that nicotinic receptor subtypes are involved in lung cancer risk, the relationship between cigarette smoke and lung cancer risk as well as the rising popularity of electronic ciga- rettes considered by many as a “safe” alternative to smoking.
    [Show full text]
  • Actions of Methoctramine, a Muscarinic M2 Receptor Antagonist, on Muscarinic and Nicotinic Cholinoceptors in Guinea-Pig Airways in Vivo and in Vitro N
    Br. J. Pharmacol. (1992), 105, 107-112 k..; Macmillan Press Ltd, 1992 Actions of methoctramine, a muscarinic M2 receptor antagonist, on muscarinic and nicotinic cholinoceptors in guinea-pig airways in vivo and in vitro N. Watson, *P.J. Barnes & 'J. Maclagan Department of Academic Pharmacology, Royal Free Hospital School of Medicine, Rowland Hill Street, London, NW3, and *Department of Thoracic Medicine, National Heart and Lung Institute, Dovehouse Street, London, SW3 1 The effects of the muscarinic M2 receptor antagonist methoctramine, on contractions of airway smooth muscle induced by cholinergic nerve stimulation and by exogenously applied acetylcholine (ACh), have been investigated in vivo and in vitro in guinea-pigs. 2 Stimulation of the preganglionic cervical vagus nerve in anaesthetized guinea-pigs, caused broncho- constriction and bradycardia which were mimicked by an intravenous dose of ACh. The muscarinic M2 antagonist, methoctramine (7-240nmolkg-1), inhibited the bradycardia induced by both vagal stimu- lation and ACh (ED50: 38 + 5 and 38 + 9nmolkg-', respectively). In this dose-range, methoctramine facilitated vagally-induced bronchoconstriction (ED50: 58 + 5nmolkg-l), despite some inhibition of ACh-induced bronchoconstriction (ED50: 81 + llnmolkg-1). The inhibition of ACh-induced broncho- constriction and hypotension was dose-dependent, but was not statistically significant until doses of 120 nmol kg'- and 240 nmol kg1- respectively. 3 In the guinea-pig isolated, innervated tracheal tube preparation, methoctramine (0.01-1 uM) caused facilitation of contractions induced by both pre- and postganglionic nerve stimulation, whereas contrac- tions induced by exogenously applied ACh were unaffected. Higher concentrations of methoctramine (> 10 M), reduced responses to both nerve stimulation and exogenous ACh, indicating blockade of post- junctional muscarinic M3 receptors.
    [Show full text]
  • Muscarine Hyperpolarizes a Subpopulation of Neurons by Activating an M, Muscarinic Receptor in Rat Nucleus Raphe Magnus in Vitro
    The Journal of Neuroscience, March 1994, 74(3): 1332-l 338 Muscarine Hyperpolarizes a Subpopulation of Neurons by Activating an M, Muscarinic Receptor in Rat Nucleus Raphe Magnus in vitro 2. Z. Pan and J. T. Williams Vellum Institute, Oregon Health Sciences University, Portland, Oregon 97201 It has been shown previously that the muscarinic cholinergic ical studies (Bowker et al., 1983; Jones et al., 1986; Sherriff et system in the nucleus raphe magnus (NRM) is involved in al., 1991). The NRM receives cholinergic afferents originating the modulation of nociception. In this study, we examined from the cells in the pedunculopontine tegmental nucleus (Rye the direct actions of muscarine on the NRM neurons in a et al., 1988). Behavioral studies have shown that local appli- slice preparation. Muscarine (I-30 PM) produced a dose- cations of both muscarinic receptor agonists and antagonists dependent hyperpolarization in a subpopulation of the NRM into the NRM causeantinociception (Brodie and Proudfit, 1986; cells that contain 5-hydroxytryptamine (5-HT). In voltage Iwamoto, 1991). In previous in vivo studies, iontophoretic ap- clamp, the muscarine-induced outward current reversed po- plication of ACh has been reported to induce an inhibition or larity at the potassium equilibrium potential and was char- excitation in the spontaneousactivity of different groups of the acterized by strong inward rectification. The reversal poten- NRM cells (Behbehani, 1982; Wessendorfand Anderson, 1983; tial was dependent on external potassium concentration, Willcockson et al., 1983; Hentall et al., 1993). Neither the cel- suggesting that the hyperpolarization induced by muscarine lular mechanismunderlying the actionsof thesecholinergic agents was mediated through an increase in an inwardly rectifying on NRM neurons nor the muscarinic receptor subtypes that potassium conductance.
    [Show full text]
  • Allosteric Modulators of G Protein-Coupled Dopamine and Serotonin Receptors: a New Class of Atypical Antipsychotics
    pharmaceuticals Review Allosteric Modulators of G Protein-Coupled Dopamine and Serotonin Receptors: A New Class of Atypical Antipsychotics Irene Fasciani 1, Francesco Petragnano 1, Gabriella Aloisi 1, Francesco Marampon 2, Marco Carli 3 , Marco Scarselli 3, Roberto Maggio 1,* and Mario Rossi 4 1 Department of Biotechnological and Applied Clinical Sciences, University of l’Aquila, 67100 L’Aquila, Italy; [email protected] (I.F.); [email protected] (F.P.); [email protected] (G.A.) 2 Department of Radiotherapy, “Sapienza” University of Rome, Policlinico Umberto I, 00161 Rome, Italy; [email protected] 3 Department of Translational Research and New Technology in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy; [email protected] (M.C.); [email protected] (M.S.) 4 Institute of Molecular Cell and Systems Biology, University of Glasgow, Glasgow G12 8QQ, UK; [email protected] * Correspondence: [email protected] Received: 26 September 2020; Accepted: 11 November 2020; Published: 14 November 2020 Abstract: Schizophrenia was first described by Emil Krapelin in the 19th century as one of the major mental illnesses causing disability worldwide. Since the introduction of chlorpromazine in 1952, strategies aimed at modifying the activity of dopamine receptors have played a major role for the treatment of schizophrenia. The introduction of atypical antipsychotics with clozapine broadened the range of potential targets for the treatment of this psychiatric disease, as they also modify the activity of the serotoninergic receptors. Interestingly, all marketed drugs for schizophrenia bind to the orthosteric binding pocket of the receptor as competitive antagonists or partial agonists.
    [Show full text]
  • BMJ Open Is Committed to Open Peer Review. As Part of This Commitment We Make the Peer Review History of Every Article We Publish Publicly Available
    BMJ Open: first published as 10.1136/bmjopen-2018-027935 on 5 May 2019. Downloaded from BMJ Open is committed to open peer review. As part of this commitment we make the peer review history of every article we publish publicly available. When an article is published we post the peer reviewers’ comments and the authors’ responses online. We also post the versions of the paper that were used during peer review. These are the versions that the peer review comments apply to. The versions of the paper that follow are the versions that were submitted during the peer review process. They are not the versions of record or the final published versions. They should not be cited or distributed as the published version of this manuscript. BMJ Open is an open access journal and the full, final, typeset and author-corrected version of record of the manuscript is available on our site with no access controls, subscription charges or pay-per-view fees (http://bmjopen.bmj.com). If you have any questions on BMJ Open’s open peer review process please email [email protected] http://bmjopen.bmj.com/ on September 26, 2021 by guest. Protected copyright. BMJ Open BMJ Open: first published as 10.1136/bmjopen-2018-027935 on 5 May 2019. Downloaded from Treatment of stable chronic obstructive pulmonary disease: a protocol for a systematic review and evidence map Journal: BMJ Open ManuscriptFor ID peerbmjopen-2018-027935 review only Article Type: Protocol Date Submitted by the 15-Nov-2018 Author: Complete List of Authors: Dobler, Claudia; Mayo Clinic, Evidence-Based Practice Center, Robert D.
    [Show full text]
  • Endothelium-Dependent Contraction and Relaxation to Acetylcholine in the Aorta of the Spontaneously Hypertensive Rat 'Chantal M
    Br. J. Pharmacol. (1994), 112, 519-524 '." © MamlaMacmillan PresPress LtdLtd,1-994-1994 Mediation by M3-muscarinic receptors of both endothelium-dependent contraction and relaxation to acetylcholine in the aorta of the spontaneously hypertensive rat 'Chantal M. Boulanger, Keith J. Morrison & Paul M. Vanhoutte Center for Experimental Therapeutics, Baylor College of Medicine, Houston, Texas 77030, U.S.A. 1 Experiments were designed to characterize the subtype(s) of endothelial muscarinic receptor that mediate(s) endothelium-dependent relaxation and contraction in the aorta of spontaneously hypertensive rats (SHR). 2 Rings of SHR aorta with endothelium were suspended in organ baths for the measurement of isometric force. Ecothiopate (an inhibitor of acetylcholinesterase) was present throughout the experiments. Endothelium-dependent contraction to acetylcholine was studied in quiescent aortic rings in the presence of N0-nitro-L-arginine (to prevent the formation of nitric oxide). Endothelium-dependent relaxation to acetylcholine was obtained during contraction to phenylephrine and in the presence of indomethacin (to inhibit cyclo-oxygenase activity). Responses to acetylcholine were assessed against the non-preferential muscarinic receptor antagonist, atropine, and the preferential antagonists pirenzepine (M,), methoctramine (M2) and 4-diphenylacetoxy-N-methylpiperidine methobromide (4-DAMP; M3). 3 The potency of acetylcholine in inducing endothelium-dependent contraction was 6.54 ± 0.07 (EC5,,). Atropine, pirenzepine, methoctramine and 4-DAMP displayed competitive antagonism towards the endothelium-dependent contraction to acetylcholine. The pA2 values for these muscarinic receptor antagonists were estimated from Arunlakshana-Schild plots to be (- logM) 9.48 ± 0.07, 6.74 ± 0.22, 6.30 ± 0.20 and 9.39 ± 0.22 respectively.
    [Show full text]
  • Inflammation Inhibits Muscarinic Signaling in in Vivo Canine Colonic
    0031-3998/02/5205-0756 PEDIATRIC RESEARCH Vol. 52, No. 5, 2002 Copyright © 2002 International Pediatric Research Foundation, Inc. Printed in U.S.A. Inflammation Inhibits Muscarinic Signaling in In Vivo Canine Colonic Circular Smooth Muscle Cells SUDARSHAN RAO JADCHERLA Sections of Neonatology, Pediatric Gastroenterology and Nutrition, Department of Pediatrics, Medical College of Wisconsin, Milwaukee, Wisconsin, U.S.A. ABSTRACT We investigated the effects of experimental colitis on the seen in normal and inflamed states, suggesting the importance of muscarinic signaling properties and contractile behavior of ca- M2 receptor. These findings suggest that changes in muscarinic nine colonic circular smooth muscle. The hypotheses that inflam- response during colitis may contribute to the abnormal motility mation 1) inhibits in vivo muscarinic receptor mediated contrac- seen with inflammatory bowel disease. (Pediatr Res 52: 756– tions, and 2) alters receptor density or receptor-binding affinities 762, 2002) were tested. Muscarine was infused close-intra-arterially in seven conscious dogs during normal and experimental colitis states. Abbreviations Colonic circular muscle contractions were recorded via surgi- Ach, acetylcholine cally attached strain gauge transducers. Muscarine stimulated AUC, area under contractions phasic contractions in a dose-dependent manner, whereas colitis 4-DAMP, 4-diphenyl acetoxy-N-methyl-piperidine methiodide was inhibited. The inhibitory concentration 50% dose of M3 Methoctramine, N, N',-bis[6[[(2-methoxyphenyl) receptor inhibitor was several times lower than that of M1,M2, methyl]amino]hexyl]-1,8-octanediamine tetrahydrochloride and M4 inhibitors during normal and colitis. However, inflam- TTX, tetrodotoxin mation induced a significant leftward shift in the circular muscle IP3, inositol (1, 4, 5,)-triphosphate inhibitory dose-response curve of M2 inhibitor.
    [Show full text]
  • [Product Monograph Template
    PRODUCT MONOGRAPH INCLUDING PATIENT MEDICATION INFORMATION N ABSTRAL® Fentanyl citrate sublingual tablets 100 mcg, 200 mcg, 300 mcg, 400 mcg, 600 mcg and 800 mcg fentanyl as fentanyl citrate Opioid Analgesic Paladin Labs Inc. Date of Preparation: 100 Alexis Nihon Blvd, Suite 600 March 8, 2018 St-Laurent, Quebec H4M 2P2 Version 7.0 Submission Control No: 210630 ® Registered trademark of Strakan International Limited ABSTRAL Product Monograph Page 1 of 64 Table of Contents PART I: HEALTH PROFESSIONAL INFORMATION .........................................................3 SUMMARY PRODUCT INFORMATION ........................................................................3 INDICATIONS AND CLINICAL USE ..............................................................................3 CONTRAINDICATIONS ...................................................................................................4 WARNINGS AND PRECAUTIONS ..................................................................................6 ADVERSE REACTIONS ..................................................................................................15 DRUG INTERACTIONS ..................................................................................................22 DOSAGE AND ADMINISTRATION ..............................................................................23 OVERDOSAGE ................................................................................................................30 ACTION AND CLINICAL PHARMACOLOGY ............................................................31
    [Show full text]
  • Muscarinic and Purinergic Signalling Within the Bladder
    Muscarinic and Purinergic Signalling within the bladder Thesis submitted to University College London for the Degree of Doctor of Medicine Samuel Bishara MBChB, MRCS, BSc (Hons) Table of Contents Acknowledgements 5 Declaration 6 Abstract 7 Chapter 1 Introduction 9 1.1 Urinary Bladder Physiology 10 1.2 Muscarinic Receptors 27 1.3 Purinergic Receptors 50 1.4 The Ussing Chamber 56 Chapter 2 Methods 62 2.1 Patient Recruitment 63 2.2 Animal Tissue Preparation 65 2.3 Organ Bath Experiments 66 2.4 Isolated Detrusor Cell experiments 69 2.5 Ussing chamber Experiments 73 Chapter 3 Results 76 3.1 Human Organ bath experiments 77 3.2 Guinea Pig organ Bath Experiments 93 3.3 Isolated detrusor Cell Experiments 106 3.4 Ussing Chamber Experiments 118 Chapter 4 Discussion 138 4.1 Clinical Impact of OAB 139 4.2 Comparison of methodologies 144 4.3 Organ bath experiments 148 4.4 Isolated detrusor cell experiments 157 4.5 The Urothelium 164 Chapter 5 Future plan of investigations 170 Chapter 6 Appendices 175 Chapter 7 references 183 2 Table of Figures Fig 1a Anatomy of the bladder 11 Fig 1b The lining of the bladder 11 Fig 1c Neurological control of the bladder 12 Fig 1d Transepithelial voltage across an epithelial surface 60 Fig 1e Ussing chamber electrical circuit 61 Fig 2a Organ Bath 68 Fig 2b Sequence of adding antagonists 68 Fig 2c Haemocytometer grid 71 Fig 2d Arrangement of electrodes in the using chamber 75 Human detrusor organ bath experiments Fig 3.0 Agonist response from a human detrusor strip 82 Fig 3.1 Repeated Carbachol dose response curves 83
    [Show full text]