Genetic Diversity and Virulence of Xanthomonas Campestris Pv
Plant Pathology (2019) 68, 1448–1457 Doi: 10.1111/ppa.13064 Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia T. Popovica* , P. Mitrovicb, A. Jelusi cc, I. Dimkicd, A. Marjanovic-Jeromela b, I. Nikolicd and S. Stankovicd aInstitute for Plant Protection and Environment, Teodora Drajzera 9,11040 Belgrade; bInstitute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad; cInstitute for Multidisciplinary Research, Kneza Viseslava 1,11000; and dUniversity of Belgrade – Faculty of Biology, Studentski Trg 16,11000 Belgrade, Serbia The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR patho- var-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respec- tively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analy- sis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts.
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