An Investigation of Protective Formulations Containing Enzyme Inhibitors Model Experiments of Trypsin

Total Page:16

File Type:pdf, Size:1020Kb

An Investigation of Protective Formulations Containing Enzyme Inhibitors Model Experiments of Trypsin UPTEC-K12018 Examensarbete 30 hp September 2012 An investigation of protective formulations containing enzyme inhibitors Model experiments of trypsin Erika Billinger Erika Billinger Svenska Cellulosa Aktiebolaget & Uppsala University 120612 AN INVESTIGATION OF PROTECTIVE FORMULATIONS CONTAINING ENZYME INHIBITORS. MODEL EXPERIMENTS ON TRYPSIN. ERIKA BILLINGER MASTER THESIS SPRING 2012 SVENSKA CELLULOSA AKTIEBOLAGET UPPSALA UNIVERSITY 2 Erika Billinger Svenska Cellulosa Aktiebolaget & Uppsala University 120612 ABSTRACT This master thesis considers an investigation of protective formulations (ointment, cream) containing enzyme inhibitors. Model experiments have been made on the enzyme trypsin. It is well accepted that feces and urine are an important causing factor for skin irritation (dermatitis) while using diaper. A protective formulation is a physical barrier that separates the harmful substances from the skin. It can also be an active barrier containing active substances, which can be active both towards the skin, and the substances from feces and urine. By preventing contact from these substances the skin will not be harmed, at least for a period of time. A number of different inhibitors were tested towards trypsin and they all showed good inhibition, two of the inhibitors were selected to be immobilized with the help of NHS- activated Sepharose. Immobilization of these two inhibitors leads to a lesser extent of the risk of developing allergy and also that the possible toxic effect can be minimized. 3 Erika Billinger Svenska Cellulosa Aktiebolaget & Uppsala University 120612 POPULÄRVETENSKAPLIG SAMMANFATTNING Det är väl känt att avföring och urin är en viktig faktor som ger upphov till irriterad hud (dermatitis) för barn som använder blöjor. Tillsammans med avföringen följer enzymer från mag-tarmkanalen med och det är dessa enzymer som ”äter” på huden och ger upphov till dermatitis. Då urin avges i blöjan stiger pH vilket leder till en mer trivsam miljö för enzymerna som i sin tur leder till en ökad aktivitet och resulterar i en större area irriterad hud. Ytterligare anledningar till dermatitis är även den fuktiga miljön som skapas av stängd miljö och även friktionen som uppkommer då rörelser sker vid användning av blöjan. En skyddande formulering, i form av exempelvis en salva eller kräm, kan separera de skadliga ämnena från huden. Man kan även tänka sig använda en aktiv barriär innehållande aktiva substanser, exempelvis inhibitorer, som kan vara skyddande mot huden och samtidigt aktiv mot de skadliga ämnena från avföring och urin. Inhibitorerna i en salva eller kräm hämmar då enzymernas aktivitet och huden skyddas och blöjeksem undviks. Genom att undvika hudkontakt med de skadliga ämnena kan huden skyddas, åtminstone under en viss tidsperiod. Då avföringen innehåller ett matspjälkningsenzym trypsin har olika inhibitorer mot enzymet undersökts. För att även minimera risken för både toxiska och allergena effekter har två av dessa inhibitorer immobiliserats på NHS-aktiverad Sepharose. 4 Erika Billinger Svenska Cellulosa Aktiebolaget & Uppsala University 120612 TABLE OF CONTENTS An investigation of protective formulations containing enzyme inhibitors. Model experiments on trypsin. Abstract ............................................................................................................................................................. 3 Populärvetenskaplig sammanfattning ................................................................................................... 4 Introduction ..................................................................................................................................................... 6 What is an enzyme ............................................................................................................................................................... 7 Enzymes in feces .................................................................................................................................................................... 7 Trypsin ....................................................................................................................................................................................... 8 Substrate for trypsin ................................................................................................................................................. 9 Inhibitors ....................................................................................................................................................... 10 Aprotinin ................................................................................................................................................................................ 10 Soybean trypsin inhibitor ............................................................................................................................................... 11 α-1-antitrypsin .................................................................................................................................................................... 12 Leupeptin ............................................................................................................................................................................... 12 Antipain .................................................................................................................................................................................. 14 Immobilization ............................................................................................................................................. 15 Schiff bases ............................................................................................................................................................................ 15 NHS-activated Sepharose ............................................................................................................................................... 17 Materials & methods .................................................................................................................................. 18 Aprotinin ................................................................................................................................................................................ 18 Soybean trypsin inhibitor ............................................................................................................................................... 18 α-1-antitrypsin .................................................................................................................................................................... 18 Leupeptin ............................................................................................................................................................................... 18 Immobilization of α-1-antitrypsin ............................................................................................................................. 19 Coupling between NHS-activated Sepharose 4 Fast Flow and α-1-antitrypsin. .......................... 19 Test of immobilization .......................................................................................................................................... 19 Immobilization of leupeptin .......................................................................................................................................... 20 Coupling between NHS-activated Sepharose 4 Fast Flow and Leupeptin ...................................... 20 Test of immobilization of leupeptin ................................................................................................................ 20 Results ............................................................................................................................................................. 20 With each inhibitor ........................................................................................................................................................... 21 Experiments of immobilized inhibitor ...................................................................................................................... 25 α-1-antitrypsin ......................................................................................................................................................... 25 Leupeptin .................................................................................................................................................................... 26 Discussion and conclusions ..................................................................................................................... 29 References ..................................................................................................................................................... 30 Acknowledgements .................................................................................................................................... 32 Appendix ........................................................................................................................................................ 33 5 Erika Billinger Svenska Cellulosa Aktiebolaget & Uppsala University 120612 INTRODUCTION It is well known that feces and urine are two factors that affect the skin in a negative way and together they can cause skin irritation, dermatitis. The biggest difference between an adult skin and a baby’s skin lies
Recommended publications
  • Understanding Drug-Drug Interactions Due to Mechanism-Based Inhibition in Clinical Practice
    pharmaceutics Review Mechanisms of CYP450 Inhibition: Understanding Drug-Drug Interactions Due to Mechanism-Based Inhibition in Clinical Practice Malavika Deodhar 1, Sweilem B Al Rihani 1 , Meghan J. Arwood 1, Lucy Darakjian 1, Pamela Dow 1 , Jacques Turgeon 1,2 and Veronique Michaud 1,2,* 1 Tabula Rasa HealthCare Precision Pharmacotherapy Research and Development Institute, Orlando, FL 32827, USA; [email protected] (M.D.); [email protected] (S.B.A.R.); [email protected] (M.J.A.); [email protected] (L.D.); [email protected] (P.D.); [email protected] (J.T.) 2 Faculty of Pharmacy, Université de Montréal, Montreal, QC H3C 3J7, Canada * Correspondence: [email protected]; Tel.: +1-856-938-8697 Received: 5 August 2020; Accepted: 31 August 2020; Published: 4 September 2020 Abstract: In an ageing society, polypharmacy has become a major public health and economic issue. Overuse of medications, especially in patients with chronic diseases, carries major health risks. One common consequence of polypharmacy is the increased emergence of adverse drug events, mainly from drug–drug interactions. The majority of currently available drugs are metabolized by CYP450 enzymes. Interactions due to shared CYP450-mediated metabolic pathways for two or more drugs are frequent, especially through reversible or irreversible CYP450 inhibition. The magnitude of these interactions depends on several factors, including varying affinity and concentration of substrates, time delay between the administration of the drugs, and mechanisms of CYP450 inhibition. Various types of CYP450 inhibition (competitive, non-competitive, mechanism-based) have been observed clinically, and interactions of these types require a distinct clinical management strategy. This review focuses on mechanism-based inhibition, which occurs when a substrate forms a reactive intermediate, creating a stable enzyme–intermediate complex that irreversibly reduces enzyme activity.
    [Show full text]
  • Potent Inhibition of Monoamine Oxidase B by a Piloquinone from Marine-Derived Streptomyces Sp. CNQ-027
    J. Microbiol. Biotechnol. (2017), 27(4), 785–790 https://doi.org/10.4014/jmb.1612.12025 Research Article Review jmb Potent Inhibition of Monoamine Oxidase B by a Piloquinone from Marine-Derived Streptomyces sp. CNQ-027 Hyun Woo Lee1, Hansol Choi2, Sang-Jip Nam2, William Fenical3, and Hoon Kim1* 1Department of Pharmacy and Research Institute of Life Pharmaceutical Sciences, Sunchon National University, Suncheon 57922, Republic of Korea 2Department of Chemistry and Nano Science, Ewha Womans University, Seoul 03760, Republic of Korea 3Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093-0204, USA Received: December 19, 2016 Revised: December 27, 2016 Two piloquinone derivatives isolated from Streptomyces sp. CNQ-027 were tested for the Accepted: January 4, 2017 inhibitory activities of two isoforms of monoamine oxidase (MAO), which catalyzes monoamine neurotransmitters. The piloquinone 4,7-dihydroxy-3-methyl-2-(4-methyl-1- oxopentyl)-6H-dibenzo[b,d]pyran-6-one (1) was found to be a highly potent inhibitor of First published online human MAO-B, with an IC50 value of 1.21 µM; in addition, it was found to be highly effective January 9, 2017 against MAO-A, with an IC50 value of 6.47 µM. Compound 1 was selective, but not extremely *Corresponding author so, for MAO-B compared with MAO-A, with a selectivity index value of 5.35. Compound 1,8- Phone: +82-61-750-3751; dihydroxy-2-methyl-3-(4-methyl-1-oxopentyl)-9,10-phenanthrenedione (2) was moderately Fax: +82-61-750-3708; effective for the inhibition of MAO-B (IC = 14.50 µM) but not for MAO-A (IC > 80 µM).
    [Show full text]
  • Evidence of Pyrimethamine and Cycloguanil Analogues As Dual Inhibitors of Trypanosoma Brucei Pteridine Reductase and Dihydrofolate Reductase
    pharmaceuticals Article Evidence of Pyrimethamine and Cycloguanil Analogues as Dual Inhibitors of Trypanosoma brucei Pteridine Reductase and Dihydrofolate Reductase Giusy Tassone 1,† , Giacomo Landi 1,†, Pasquale Linciano 2,† , Valeria Francesconi 3 , Michele Tonelli 3 , Lorenzo Tagliazucchi 2 , Maria Paola Costi 2 , Stefano Mangani 1 and Cecilia Pozzi 1,* 1 Department of Biotechnology, Chemistry and Pharmacy, Department of Excellence 2018–2022, University of Siena, via Aldo Moro 2, 53100 Siena, Italy; [email protected] (G.T.); [email protected] (G.L.); [email protected] (S.M.) 2 Department of Life Science, University of Modena and Reggio Emilia, via Campi 103, 41125 Modena, Italy; [email protected] (P.L.); [email protected] (L.T.); [email protected] (M.P.C.) 3 Department of Pharmacy, University of Genoa, Viale Benedetto XV n.3, 16132 Genoa, Italy; [email protected] (V.F.); [email protected] (M.T.) * Correspondence: [email protected]; Tel.: +39-0577-232132 † These authors contributed equally to this work. Abstract: Trypanosoma and Leishmania parasites are the etiological agents of various threatening Citation: Tassone, G.; Landi, G.; neglected tropical diseases (NTDs), including human African trypanosomiasis (HAT), Chagas disease, Linciano, P.; Francesconi, V.; Tonelli, and various types of leishmaniasis. Recently, meaningful progresses in the treatment of HAT, due to M.; Tagliazucchi, L.; Costi, M.P.; Trypanosoma brucei (Tb), have been achieved by the introduction of fexinidazole and the combination Mangani, S.; Pozzi, C. Evidence of therapy eflornithine–nifurtimox. Nevertheless, due to drug resistance issues and the exitance of Pyrimethamine and Cycloguanil animal reservoirs, the development of new NTD treatments is still required.
    [Show full text]
  • Insights Into the Role of Tick Salivary Protease Inhibitors During Ectoparasite–Host Crosstalk
    International Journal of Molecular Sciences Review Insights into the Role of Tick Salivary Protease Inhibitors during Ectoparasite–Host Crosstalk Mohamed Amine Jmel 1,† , Hajer Aounallah 2,3,† , Chaima Bensaoud 1, Imen Mekki 1,4, JindˇrichChmelaˇr 4, Fernanda Faria 3 , Youmna M’ghirbi 2 and Michalis Kotsyfakis 1,* 1 Laboratory of Genomics and Proteomics of Disease Vectors, Biology Centre CAS, Institute of Parasitology, Branišovská 1160/31, 37005 Ceskˇ é Budˇejovice,Czech Republic; [email protected] (M.A.J.); [email protected] (C.B.); [email protected] (I.M.) 2 Institut Pasteur de Tunis, Université de Tunis El Manar, LR19IPTX, Service d’Entomologie Médicale, Tunis 1002, Tunisia; [email protected] (H.A.); [email protected] (Y.M.) 3 Innovation and Development Laboratory, Innovation and Development Center, Instituto Butantan, São Paulo 05503-900, Brazil; [email protected] 4 Faculty of Science, University of South Bohemia in Ceskˇ é Budˇejovice, 37005 Ceskˇ é Budˇejovice, Czech Republic; [email protected] * Correspondence: [email protected] † These authors contributed equally. Abstract: Protease inhibitors (PIs) are ubiquitous regulatory proteins present in all kingdoms. They play crucial tasks in controlling biological processes directed by proteases which, if not tightly regulated, can damage the host organism. PIs can be classified according to their targeted proteases or their mechanism of action. The functions of many PIs have now been characterized and are showing clinical relevance for the treatment of human diseases such as arthritis, hepatitis, cancer, AIDS, and cardiovascular diseases, amongst others. Other PIs have potential use in agriculture as insecticides, anti-fungal, and antibacterial agents.
    [Show full text]
  • Sequence Requirement for Peptide Recognition by Rat Brain P2lras Protein Farnesyltransferase
    Proc. Natl. Acad. Sci. USA Vol. 88, pp. 732-736, February 1991 Biochemistry Sequence requirement for peptide recognition by rat brain p2lras protein farnesyltransferase (covalent modification/prenylation/mevalonate/tetrapeptides/enzyme inhibition) YUVAL REISS*, SARAH J. STRADLEYt, LILA M. GIERASCHt, MICHAEL S. BROWN*, AND JOSEPH L. GOLDSTEIN* Departments of *Molecular Genetics and tPharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235 Contributed by Michael S. Brown, October 30, 1990 ABSTRACT We tested 42 tetrapeptides for their ability to a heterodimer (10, 11). The enzyme recognizes sequences as bind to the rat brain p2l' protein farnesyltransferase as short as four amino acids provided that cysteine is at the estimated by their ability to compete with p2lH"- in a farnesyl- fourth position from the COOH terminus. Recognition was transfer assay. Peptides with the highest affinity had the struc- demonstrated by the ability ofthese peptides to compete with ture Cys-Al-A2-X, where positions Al and A2 are occupied by p21Ha-ras in the protein farnesyltransferase assay. In addition, aliphatic amino acids and position X is-occupied by a COOH- the enzyme adhered to an affinity column containing a terminal methionine, serine, or phenylalanine. Charged resi- hexapeptide corresponding to the COOH-terminal sequence dues reduced affinity slightly at the Al position and much more of p2lKi-msB. A similar enzymatic activity was identified by drastically at the A2 and X positions. Effective inhibitors in- Schaber et al. (12) in crude extracts of bovine brain. cluded tetrapeptides corresponding to the COOH termini of all The above findings suggest that the recognition site for the animal cell proteins known to be farnesylated.
    [Show full text]
  • Angiotensin-I-Converting Enzyme Inhibitory Activity of Coumarins from Angelica Decursiva
    molecules Article Angiotensin-I-Converting Enzyme Inhibitory Activity of Coumarins from Angelica decursiva 1,2,3,4, 1, 5, 1, Md Yousof Ali y, Su Hui Seong y , Hyun Ah Jung * and Jae Sue Choi * 1 Department of Food and Life Science, Pukyong National University, Busan 48513, Korea; [email protected] (M.Y.A.); [email protected] (S.H.S.) 2 Department of Chemistry and Biochemistry, Concordia University, Montreal, QC H4B 1R6, Canada 3 Department of Biology, Faculty of Arts and Science, Concordia University, 7141 Sherbrooke St. W., Montreal, QC H4B 1R6, Canada 4 Centre for Structural and Functional Genomic, Department of Biology, Faculty of Arts and Science, Concordia University, 7141 Sherbrooke St. W., Montreal, QC H4B 1R6, Canada 5 Department of Food Science and Human Nutrition, Jeonbuk National University, Jeonju 54896, Korea * Correspondence: [email protected] (H.A.J.); [email protected] (J.S.C.); Tel.: +82-63-270-4882 (H.A.J.); +82-51-629-5845 (J.S.C.) These authors contributed equally to this work. y Academic Editor: Rachid Skouta Received: 11 October 2019; Accepted: 30 October 2019; Published: 31 October 2019 Abstract: The bioactivity of ten traditional Korean Angelica species were screened by angiotensin-converting enzyme (ACE) assay in vitro. Among the crude extracts, the methanol extract of Angelica decursiva whole plants exhibited potent inhibitory effects against ACE. In addition, the ACE inhibitory activity of coumarins 1–5, 8–18 was evaluated, along with two phenolic acids (6, 7) obtained from A. decursiva. Among profound coumarins, 11–18 were determined to manifest marked inhibitory activity against ACE with IC50 values of 4.68–20.04 µM.
    [Show full text]
  • Bacterial Tropone Natural Products and Derivatives
    Reviews ChemBioChem doi.org/10.1002/cbic.201900786 1 2 3 Bacterial Tropone Natural Products and Derivatives: 4 5 Overview of their Biosynthesis, Bioactivities, Ecological Role 6 and Biotechnological Potential 7 8 Ying Duan,[a] Melanie Petzold,[a] Raspudin Saleem-Batcha,[a] and Robin Teufel*[a] 9 10 11 Tropone natural products are non-benzene aromatic com- sulfur among other reactions. Tropones play important roles in 12 pounds of significant ecological and pharmaceutical interest. the terrestrial and marine environment where they act as 13 Herein, we highlight current knowledge on bacterial tropones antibiotics, algaecides, or quorum sensing signals, while their 14 and their derivatives such as tropolones, tropodithietic acid, bacterial producers are often involved in symbiotic interactions 15 and roseobacticides. Their unusual biosynthesis depends on a with plants and marine invertebrates (e.g., algae, corals, 16 universal CoA-bound precursor featuring a seven-membered sponges, or mollusks). Because of their potent bioactivities and 17 carbon ring as backbone, which is generated by a side reaction of slowly developing bacterial resistance, tropones and their 18 of the phenylacetic acid catabolic pathway. Enzymes encoded derivatives hold great promise for biomedical or biotechnolog- 19 by separate gene clusters then further modify this key ical applications, for instance as antibiotics in (shell)fish 20 intermediate by oxidation, CoA-release, or incorporation of aquaculture. 21 22 23 1. Introduction pathways and unforeseen enzyme reactions may impair 24 detection and structural predictions.[3] 25 Structurally diverse natural products are typically generated by A remarkable case of such a noncanonical biosynthetic 26 dedicated secondary metabolic pathways that mostly operate route is found for bacterial tropone (9) natural products and 27 in microbes and plants.
    [Show full text]
  • Correction for Ahmad Et Al., Potent Competitive Inhibition of Human
    Correction BIOCHEMISTRY Correction for “Potent competitive inhibition of human ribo- nucleotide reductase by a nonnucleoside small molecule,” by Md. Faiz Ahmad, Intekhab Alam, Sarah E. Huff, John Pink, Sheryl A. Flanagan, Donna Shewach, Tessianna A. Misko, Nancy L. Oleinick, William E. Harte, Rajesh Viswanathan, Michael E. Harris, and Chris Godfrey Dealwis, which was first published July 17, 2017; 10.1073/pnas.1620220114 (Proc. Natl. Acad. Sci. U.S.A. 114, 8241–8246). The authors note, on page 8243, right column, first paragraph, line 9, “The C7 atom of the hydrazone backbone is an isosteric center that adopts the transisomer configuration (Fig. 2C)” should instead read, “The C7 atom of the hydrazone backbone is an isosteric center that adopts the Z-isomer configuration (Fig. 2C).” Published under the PNAS license. First published November 9, 2020. www.pnas.org/cgi/doi/10.1073/pnas.2021828117 30858 | PNAS | December 1, 2020 | vol. 117 | no. 48 www.pnas.org Downloaded by guest on September 27, 2021 Potent competitive inhibition of human ribonucleotide reductase by a nonnucleoside small molecule Md. Faiz Ahmada,1, Intekhab Alama,1, Sarah E. Huffb,1, John Pinkc, Sheryl A. Flanagand, Donna Shewachd, Tessianna A. Miskoa, Nancy L. Oleinickc,e, William E. Hartea, Rajesh Viswanathanb, Michael E. Harrisf, and Chris Godfrey Dealwisa,g,2 aDepartment of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, OH 44106; bDepartment of Chemistry, Case Western Reserve University, Cleveland, OH 44106; cCase Comprehensive Cancer Center,
    [Show full text]
  • Biological Chemistry I: Enzymes Kinetics and Enzyme Inhibition
    Chemistry 5.07SC Biological Chemistry I Fall Semester, 2013 Lectures 7 and 8 Enzyme Kinetics (I) and Enzyme Inhibition (II) Go back and review chemical kinetics that you were introduced to in Freshman Chemistry. Also read Chapter 12 in the course textbook. I. How do enzymatic reactions and chemically catalyzed reactions differ from uncatalyzed chemical reactions? Figure by O'Reilly Science Art for MIT OpenCourseWare. Figure 1. Experimental observations for a typical enzyme catalyzed reaction (left) and a typical uncatalyzed chemical reaction (right). On the left, the reaction becomes zero order in substrate as the enzyme active site is saturated. On the right, no saturation is observed and the rate continues to be proportional to the concentration of substrate ([S]). For enzymatic reactions (or any catalytic reactions in general), the initial rate of the reaction is proportional to [S], as it is for the uncatalyzed reaction (Figure 1). However, at high [S], the reaction becomes zero order in [S], that is the rate of product formation is independent of the [S]. The active site of the enzyme is 100% saturated with S, thus increasing [S] has no effect on the rate of product formation. How can we mathematically describe the experimental observations? The following is the 1 simplest description of an enzymatic reaction where a single substrate is converted to product. Most enzymatic reactions involve two or three substrates (products). The analysis described below, however, is the same regardless of the number of substrates/products. The algebra is more complex. Digression about Enzyme Assays. Conditions in vitro for assays: 1.
    [Show full text]
  • Spring 2013 Lecture 16 & 17
    CHM333 LECTURES 16 & 17: 2/22 – 25/13 SPRING 2013 Professor Christine Hrycyna ENZYME INHIBITION - INHIBITORS: • Interfere with the action of an enzyme • Decrease the rates of their catalysis • Inhibitors are a great focus of many drug companies – want to develop compounds to prevent/control certain diseases due to an enzymatic activity 1. e.g. AIDS and HIV protease inhibitors • HIV protease essential for processing of proteins in virus • Without these proteins, viable viruses cannot be released to cause further infection - Inhibitors can be REVERSIBLE or IRREVERSIBLE • Irreversible Inhibitors o Enzyme is COVALENTLY modified after interaction with inhibitor o Derivatized enzyme is NO longer a catalyst – loses enzymatic activity o Original activity cannot be regenerated o Also called SUICIDE INHIBITORS § e.g. nerve gas, VX gas § Aspirin! Acetylates Ser in active site of cyclooxygenase (COX) enzyme • Reversible Inhibitors o Bind to enzyme and are subsequently released o Leave enzyme in original condition o Three subclasses: § Competitive Inhibitors § Non-competitive Inhibitors § Uncompetitive Inhibitors o Can be distinguished by their kinetics of inhibition How are inhibitors characterized experimentally? • First, perform experiment without inhibitor o Measure velocity at different substrate concentrations, keeping [E] constant. Choose values of [S] o Get [S] and V values from experiment o Take reciprocal of [S] and V values (“1 over”) o Plot on graph 1/[S] (x) vs. 1/V (y) – don’t use Michaelis-Menten – not reliable • Second, do the SAME experiment in parallel using a fixed amount of inhibitor and same values for E and S • Get V values and plot together with uninhibited reaction • Depending on how the graph looks and using the subsequent equation of the line we can: o Determine type of inhibition (competitive vs.
    [Show full text]
  • Inhibition of Phosphoribosylaminoimidazolecarboxamide Transformylase by Methotrexate and Dihydrofolic
    Proc. Nati. Acad. Sci. USA Vol. 82, pp. 4881-4885, August 1985 Biochemistry Inhibition of phosphoribosylaminoimidazolecarboxamide transformylase by methotrexate and dihydrofolic acid polyglutamates (antimetabolites/breast cancer/purine synthesis/folic acid/enzyme kinetics) CARMEN J. ALLEGRA, JAMES C. DRAKE, JACQUES JOLIVET, AND BRUCE A. CHABNER Cliqical Pharmacology Branch, Division of Cancer Treatment, National Cancer Institute, Bethesda, MD 20205 Communicated by DeWitt Stetten, Jr., March 13, 1985 ABSTRACT We report the enhanced inhibitory potency of describes the 2500-fold enhanced capacity of MTX methotrexate (MTX) polyglutamates and dihydrofolate polyglutamate to inhibit 10-formyltetrahydrofolate:5'-phos- pentaglutamate on the catalytic activity of phosphoribosylami- phoribosyl-5-amino-4-imidazolecarboxamide formyl- noimidazolecarboxamide (AICAR) transformylase purified transferase [5-amino-4-imidazolecarboxamide ribotide from MCF-7 human breast cancer cells. In the present work, (AICAR) transformylase, EC 2.1.2.3; AICAR TFase], a MTX (4-amino-10-methylpteroylglutamic acid) and folate-requiring enzyme that catalyzes the reaction: 10- dihydrofolate, both monoglutamates, were found to be weak formyl-tetrahydrofolate (10-formyl-H4PteGlu) + AICAR, competitive inhibitors ofAICAR transformylase with Kis of 143 yielding 5'-phosphoribosyl-5-formamido-4-imidazole-car- and 63 ,uM, respectively, and their inhibitory capacity was boxamide (formyl-AICAR), an intermediate in the de novo largely unaffected by the glutamated state of the folate purine biosynthetic pathway, and tetrahydrofolic acid cosubstrate. In contrast, MTX polyglutamates were found to (H4PteGlu). We also report that H2PteGlu5, which increases be potent competitive inhibitors, with an =10-fold increase in in the cell following inhibition of H2PteGlu reductase (11), inhibitory potency with the addition of each glutamate group potently inhibits AICAR TFase.
    [Show full text]
  • Further Studies of the Action of Disulfiram And
    Biochem. J. (1982) 203, 743-754 743 Printed in Great Britain Further studies ofthe action of disulfiram and 2,2'-dithiodipyridine on the dehydrogenase and esterase activities of sheep liver cytoplasmic aldehyde dehydrogenase Trevor M. KITSON* Department of Chemistry, Biochemistry and Biophysics, Massey University, Palmerston North, New Zealand, and Department ofBiochemistry, University ofHull, Hull HU6 7RX, U.K. (Received 19 January 1982/Accepted 16 February 1982) 1. Pre-modification of cytoplasmic aldehyde dehydrogenase by disulfiram results in the same extent of inactivation when the enzyme is subsequently assayed as a dehydrogenase or as an esterase. 2. 4-Nitrophenyl acetate protects the enzyme against inactivation by disulfiram, particularly well in the absence of NAD+. Some protection is also provided by chloral hydrate and indol-3-ylacetaldehyde (in the absence of NAD+). 3. When disulfiram is prevented from reacting at its usual site by the presence of 4-nitrophenyl acetate, it reacts elsewhere on the enzyme molecule without causing inactivation. 4. Enzyme in the presence of aldehyde and NAD+ is not at all protected against disulfiram. It is proposed that, under these circumstances, disulfiram reacts with the enzyme-NADH complex formed in the enzyme-catalysed reaction. 5. Modification by disulfiram results in a decrease in the amplitude of the burst of NADH formation during the dehydrogenase reaction, as well as a decrease in the steady-state rate. 6. 2,2'-Dithiodipyridine reacts with the enzyme both in the absence and presence of NAD+. Under the former circumstances the activity of the enzyme is little affected, but when the reaction is conducted in the presence of NAD+ the enzyme is activated by approximately 2-fold and is then relatively insensitive to the inactivatory effect of disulfiram.
    [Show full text]