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Annex 1: Field Protocol

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Annex 1: Field Protocol Annex 1: Field protocol Annex 1-Table 1: Fish data Fish data Date (dd/mm/yyyy) Site code Sampling method Site name Sampling location Name of watercourse Main river region XY co-ordinates GPS co-ordinates Longitude Transect length [m] Latitude Fished area [m2] Altitude[m] Wetted width [m] Fish data Fish species number of specimen number of specimen length ≤150 mm length >150 mm 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 Annex 1-Table 2: Abiotic and method data Reference fields Site code Date dd/mm/yyyy Site name Name of watercourse or River name River region XY co-ordinates or longitude, latitude GPS co-ordinates Altitude m Sampling location Main channel/Backwaters/Mixed Sampling method Boat/Wading Fished area m2 Wetted width m Abiotic variables Altitude m Lakes upstream affecting Yes / No the site Distance from source km Flow regime Permanent / Summer dry/Winter dry/Intermittent Geomophology Naturally constrained no modification/ Braided/Sinuous/Meand regular/Meand tortuous Former floodplain No/Small/Medium/Large/Some waterbodies remaining/ Water source type Glacial/Nival/Pluvial/Groundwater Former sediment size Organic/Silt/Sand/ Gravel-Pebble-Cobble/Boulder-Rock Upstream drainage area km2 Distance from source km River slope m/km (‰) Mean air temperature Celsius (°C) Annex 1-Table 3: Human impact data (optional) Site code hChemical data Name watercourse Oxygen (mg/l or % saturation) XYZ co-ordinates or X:……………………(N) Conductivity (mS/cm) longitude latitude altitude Y:……………………(E) Z :…………………….m Site name pH Main river region Phosphate (mg/l) Field responsible Nitrite (mg/l) GPS co-ordinates Nitrate (mg/l) Sampling hour Turbidity (NTU) Secchi depth (cm) Site characteristics Water level low / normal / high Natural shelters natural / poorly developed / absent Pools in transect number: Riffles in transect number: Meanders in transect number: Bank natural / partly artificial / overall reinforced Morphological condition Minor / slight / moderate / strong / severe / disturbance Agricultural activities range: 50 m from stream: none / along one bank / along both sides Prairies range: 50 m from stream: none / along one bank / along both sides Trees range: 20 m from stream: none / < 10 / >10 & < 50 / > 50 or forest Urbanisation range: 100 m from stream: none / < 5 / >5 & <10 / >10 (constructions) Industrial activities Yes: describe…………………………..……………..…./ none Water flow Minor / slight / moderate / strong / severe / disturbance Impact of agriculture in > 40% cultivated land, severe impact: class 5; river basin upstream > 40% cultivated land, strong impact: class 4; < 40% cultivated land, moderate impact: class 3; < 40% cultivated land, low impact: class 2; < 10% cultivated land: class 1; Urbanisation in river basin >15% urban land severe impact: class 5 upstream >15% urban land strong impact: class 4 <15% urban land moderate impact: class 3 <15% urban land low impact: class 2 <1% urban land: class 1 Migration barriers Yes: describe…………………………..……………..…./ none (transect) Dam present upstream Yes / No Migration barriers in the Barriers present: no passage for fish possible: class 5 river (total river) passage for 1 single species occasionally: class 4 passage for certain species or certain years: class 3 passage for most species most years: class 2 No barriers (or functional bypass): class 1 Annex 2 - River groups, Ecoregions and Mediterranean types Annex 2-Table 1: Main European River Regions: 47 hydrological units (large basins and IHBS Sea areas), based on similarities between native fish fauna Adriatic Sea (continental coast) Medway Adriatic Sea (peninsular coast) Mersey Baltic Sea (continental coast) Meuse Baltic Sea (peninsular coast) Nemunas Bay of Biscay (French coast) North Atlantic Ocean Bay of Biscay (Spanish coast) North Sea (continental coast) Bristol Channel North Sea (insular coast) Danube Odra Douro Rhine Ebro Rhone Elbe Seine English Channel (continental coast) Severn English Channel (insular coast) Skagerrak Garonne Tagus Great Ouse Tees Guadalquivir Thames Guadiana Trent Gulf of Finland Tyne Gulf of Riga Tyrrhenian Sea Irish Sea Wear Kattegat Weser Loire Wisla Mediterranean Sea (French coast) Yorkshire Ouse Mediterranean Sea (Spanish coast) Annex 2-Figure 1: Assignment of 47 river regions identified in the EFI+ project Annex 2-Table 2: Ecoregion name and number according to Illies and the WFD* Ecoregion according to WFD 1 Iberian Peninsula 14 Central Plains 2 Pyrenees 15 Baltic Province 3 Italy 16 Eastern Plains 4 Alps 17 Ireland 5 Dinarian Western Balkan 18 Great Britain 6 Hellenic Western Balkan 19 Iceland 7 Eastern Balkan 20 Borealic Uplands 8 Western Highlands 21 Tundra 9 Central Highlands 22 Fenno-Scandian Shield 10 The Carpathians 23 Taiga 11 Hungarian Lowlands 24 The Caucasus 12 Pontic Province 25 Caspic Depression 13 Western Plains 21 19 23 22 20 15 16 17 14 18 9 10 13 8 11 4 12 5 2 1 3 7 6 Annex 2-Figure 2: Ecoregions according to Illies and WFD in Europe* * Note: Not all of the ecoregions shown above are covered by EFI+. To get the regions covered by the project see Table 6 in the main text of the manual. Annex 2-Figure 3: Mediterranean types in Europe: Brown is Mediterranean type 1; Yellow is Mediterranean type 2 Annex 3: Fish species in EFI software Annex 3 – Table 1 id Species id Species 1 Abramis ballerus 26 Ameiurus nebulosus 2 Abramis bjoerkna 27 Ameiurus punctatus 3 Abramis brama 28 Anaecypris hispanica 4 Abramis sapa 29 Anguilla anguilla 5 Achondrostoma arcasii 30 Aphanius fasciatus 6 Achondrostoma occidentale 31 Aphanius iberus 7 Achondrostoma oligolepis 32 Aristichthys nobilis 8 Acipenser baeri 33 Aspius aspius 9 Acipenser gueldenstaedtii 34 Atherina boyeri 10 Acipenser naccarii 35 Atherina presbyter 11 Acipenser nudiventris 36 Barbatula barbatula 12 Acipenser oxyrinchus 37 Barbatula bureschi 13 Acipenser ruthenus 38 Barbaus carpaticus 14 Acipenser stellatus 39 Barbus albanicus 15 Acipenser sturio 40 Barbus barbus 16 Alburnoides bipunctatus 41 Barbus bocagei 17 Alburnus albidus 42 Barbus caninus 18 Alburnus alburnus 43 Barbus comizo 19 Alburnus alburnus alborella 44 Barbus cyclolepis 20 Alosa agone 45 Barbus euboicus 21 Alosa alosa 46 Barbus graecus 22 Alosa fallax 47 Barbus graellsii 23 Alosa macedonica 48 Barbus guiraonis 24 Alosa immaculata 49 Barbus haasi 25 Ameiurus melas 50 Barbus meridionalis id Species id Species 51 Barbus microcephalus 76 Cobitis elongatoides 52 Barbus peloponnesius 77 Cobitis hellenica 53 Barbus petenyi 78 Cobitis megaspila 54 Barbus plebejus 79 Cobitis meridionalis 55 Barbus prespensis 80 Cobitis paludica 56 Barbus sclateri 81 Cobitis taenia 57 Barbus tyberinus 82 Cobitis vettonica 58 Benthophiloides brauneri 83 Coregonus albula 59 Benthophilus stellatus 84 Coregonus autumnalis 60 Carassius auratus 85 Coregonus lavaretus 61 Carassius carassius 86 Coregonus maraena 62 Carassius gibelio 87 Coregonus oxyrinchus 63 Chalcalburnus chalcoides 88 Coregonus peled 64 Chelon labrosus 89 Coregonus pidschian 65 Chondrostoma arrigonis 90 Cottus gobio 66 Chondrostoma genei 91 Cottus koshewniko 67 Chondrostoma miegii 92 Cottus petiti 68 Chondrostoma nasus 93 Cottus poecilopus 69 Chondrostoma soetta 94 Ctenopharyngodon idella 70 Chondrostoma toxostoma 95 Cyprinus carpio 71 Chondrostoma turiense 96 Dicentrarchus labrax 72 Clarias gariepinus 97 Economidichthys pygmaeus 73 Clupeonella cultriventris 98 Economidichthys trichonis 74 Cobitis calderoni 99 Esox lucius 75 Cobitis elongata 100 Eudontomyzon danfordi id Species id Species 101 Eudontomyzon mariae 126 Iberocypris palaciosi 102 Eudontomyzon vladykovi 127 Knipowitschia cameliae 103 Eupallasella perenurus 128 Knipowitschia caucasica 104 Fundulus heteroclitus 129 Knipowitschia panizzae 105 Gambusia affinis 130 Knipowitschia punctatissima 106 Gambusia holbrooki 131 Knipowitschia thessala 107 Gasterosteus aculeatus 132 Ladigesocypris ghigii 108 Gasterosteus crenobiontus 133 Lampetra fluviatilis 109 Gasterosteus gymnurus 134 Lampetra planeri 111 Gobio gobio 135 Lepomis gibbosus 112 Gobio kesslerii 136 Lethenteron camtschaticum 113 Gobio lozanoi 137 Lethenteron zanandreai 114 Gobio uranoscopus 138 Leucaspius delineatus 115 Gymnocephalus baloni 139 Leuciscus borysthenicus 116 Gymnocephalus cernuus 140 Leuciscus cephalus 117 Gymnocephalus schraetser 141 Leuciscus idus 118 Hemichromis fasciatus 142 Leuciscus keadicus 119 Australoheros facetus 143 Leuciscus leuciscus 120 Hucho hucho 144 Leuciscus lucumonis 121 Huso huso 145 Leuciscus muticellus 122 Hypophthalmichthys molitrix 146 Leuciscus pleurobipunctatus 123 Iberochondrostoma almacai 147 Leuciscus souffia 124 Iberochondrostoma lemmingii 148 Leuciscus svallize 125 Iberochondrostoma lusitanicum 149 Liza aurata 150 Liza ramada id Species id Species 151 Liza saliens 176 Petromyzon marinus 152 Lota lota 177 Phoxinus phoxinus 153 Micropterus salmoides 178 Pimephales promelas 154 Misgurnus anguillicaudatus 179 Platichthys flesus 155 Misgurnus fossilis 180 Pleuronectes platessa 156 Mugil cephalus 181 Poecilia reticulata 157 Mylopharyngodon piceus 182 Polyodon spathula 158 Neogobius fluviatilis 183 Pomatoschistus microps 159 Neogobius gymnotrachelus 184 Pomatoschistus minutus 160 Neogobius kessleri 185 Proterorhinus marmoratus 161 Neogobius melanostomus 186 Pseudochondrostoma duriense 162 Neogobius syrman 187 Pseudochondrostoma polylepis 163 Oncorhynchus gorbuscha 188 Pseudochondrostoma willkommii 164 Oncorhynchus kisutch 189 Pseudophoxinus beoticus 165 Oncorhynchus mykiss 190 Pseudophoxinus stymphalicus 166 Oncorhynchus tschawytscha 191 Pseudorasbora
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