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Tuberculosis Diagnosis Complete range of products for Tuberculosis Diagnosis Fast, Economical, Easy, Reliable and Confirmatory In world’s most cost effective manner SL001 Growth within 12-15 days HiMediaLaboratories™ himedialabs.com Tuberculosis Diagnosis Tuberculosis has been a phenomenal foe to mankind and has taken its toll on human life over several centuries. Being the most common cause of death world over due to a single infectious agent in adults, tuberculosis accounts for over a quarter of all avoidable dealths globally. The resurgence of tuberculosis as the “twin-disaster” of co-infection with the Human Immunodeficiency Virus (HIV) and the occurrence of the multi-drug resistant tuberculosis is demanding for focused attention of the clinicians, society and the health administrators. Clinical microbiology laboratories can play an important role in the control of the spread of tuberculosis through the timely detection, isolation, identification and drug susceptiility testing of Mycobacterium tuberculosis. HiMedia offers the varied range of products required for the complete diagnosis of tuberculosis, right from specimen collection containers upto drug sensitivity testing and confirmative Biochemical Test Kits. The prepared, ready to use L. J. Media Slants, support confirmed growth of Mycobacterium tuberculosis bacteria after incubation. Thus helping in early diagnosis and treatment of Tuberculosis. HiMedia’s Media Slants is “THE” test to be done if clinician is suspecting a positive case of TB. Complete comprehensive product range for isolation, cultivation and biochemical identification of M. tuberculosis y Sample Collection y Ready Prepared L. J. Medium slants, plain y Sample Processing, Sputum Digestion & with antibiotics for Drug Susceptibility Testing y Enriching Sample with Growth and Selective y supplements Staining Kits for Diagnosis of Mycobacteria y y Cultivating and Isolating Mycobacteria on Biochemical Identification Kit for Selective Media (In Powder form & Prepared Confirmation of Mycobacteria form) y Lyophlized Culture from Microbilogics, USA Tuberculosis Diagnostics Complete range of products for Tuberculosis Diagnosis involving processing steps Sample Collection Sample Processing (Sputum digestion) Enrichment of Sample (Growth & Selective supplements) Cultivation & Isolation (Selective Media) (In Powder, Granulated & Prepared form) Drug susceptibility testing (L.J.Medium slants with antibiotics) Biochemical Identification Kits for confirmation of Mycobacteria & Staining methods for Presumptive Diagnosis For Sputum Sample processing Reagents for Sputum Digestion and Decontamination *FD118 Mucasol For optimal recovery of mycobacteria from clinical specimens, releasing mycobacteria trapped in mucin and cells due to sputum liquefying action of DTT (dithiothreitol). *FD173 / FD173B Mycoprep A Combination liquefaction and decontamination mixture, containing: NALC (N-acetyl L-cysteine alkali digestant) 2% Sodium hydroxide-citrate and phosphate buffer. Eliminates contaminating bacteria and digests sputum. *FD260 Penta Mix An antibiotic mixture recommended to add in media to reduce contamination of other organisms from suspected tuberculosis positive clinical samples prior to inoculation. If desired can be added to M198 (Middlebrook 7H9 Broth Base). FD260 Penta Mix ; Contains Polymyxin B, Amphotericin B, Nalidixic acid, Trimethoprim & Azolectin 1 For Enrichment, Cultivation and Isolation Using selective media (in powder form and prepared form) along with growth and selective supplements Dehydrated Culture Media w/ supplements wherever required M553 Antibiotic Assay Medium G M179 Dubos Oleic Agar Base For microbiological assay of Bleomycin sulphate For cultivation of Mycobacteria using Mycobacterium smegmatis, as a test organism FD020* Oleic Albumin Supplement ME553 Antibiotic Assay Medium G MV179 Dubos HiVeg™ Broth Base For the microbiological assay of Bleomycin using For usage & supplement refer M179 Mycobacterium smegmatis, as a test organism in accordance with EP M839 Dubos Oleic Broth Base Mycobacteria M553B Antibiotic Assay Medium G For cultivation of For the microbiological assay of Bleomycin using FD020* Oleic Albumin Supplement Mycobacterium smegmatis, as a test organism in MV839 Dubos Oleic HiVeg™ Broth Base accordance with BP For usage, supplement refer M839 M797 Antibiotic Assay Medium No. 34 M247 IUT Medium Base For preparation of suspension of Mycobacterium For cultivation of Mycobacterium tuberculosis smegmatis used as the test organism for the assay of Bleomycin M161 Kirchner Medium Base, Modified For cultivation of Mycobacterium tuberculosis MU797 Antibiotic Assay Medium No. 34 Used as a suspending medium for Mycobacterium M162 Lowenstein Jensen Medium Base (L.J. Medium) smegmatis, which is used as a test organism in the For isolation and cultivation of Mycobacterium microbiological assay of Bleomycin in accordance species with USP FD053* Gruft Mycobacterial Supplement M798 Antibiotic Assay Medium No. 35 GM162 Lowenstein Jensen Medium Base, Granulated For microbiological assay of Bleomycin using (L.J. Medium, Granulated) Mycobacterium smegmatis For usage & supplement refer M162 MV798 Antibiotic HiVeg™ Assay Medium No. 35 For usage refer M798 MM162 Lowenstein-Jensen Medium (L.J. Medium) (Twin Pack) MU798 Antibiotic Assay Medium No. 35 In accordance with IP for isolation and cultivation For the microbiological assay of Bleomycin using of Mycobacterium species Mycobacterium smegmatis, as a test organism in FD053* Gruft Mycobacterial Supplement accordance with USP M2032 L.J. Medium Modified MM798 Antibiotic Assay Medium I Used for the isolation of Mycobacterium species Used for the microbiological assay of Bleomycin from mixed flora using Mycobacterium smegmatis, as a test organism FD338* LCN Supplement in accordance with IP M1542 Lowenstein Jensen Medium Base w/o Starch MM1276 Dilute Sautans Medium (Twin Pack) For drug resistance testing of Mycobacteria in For cultivation and enumeration of Mycobacteria, in accordance with WHO. accordance with IP M197 Middlebrook 7H9 Agar Base M067 Dubos Broth Base For isolation, cultivation and sensitivity testing of For preparation of liquid medium for rapid Mycobacterium tuberculosis. cultivation of pure cultures of Mycobacterium tuberculosis and related microorganisms FD018* Middlebrook OADC Growth Supplement FD201* Albumin Glucose Supplement FD348* OADS Supplement MV067 Dubos HiVeg™ Broth Base For usage, supplement refer M067 On receipt all the above products to be stored at 10-30oC *On receipt, store between 2-80C 2 M198 Middlebrook 7H9 Broth Base M867 Peizer TB Medium Base For cultivation and sensitivity testing of for cultivation of Mycobacterium tuberculosis. Mycobacterium tuberculosis. M1276 Sauton’s Fluid Medium Base FD019* Middlebrook ADC Growth Supplement For cultivation and enumeration of Mycobacteria, in M199 Middlebrook 7H10 Agar Base accordance with IP. For isolation, cultivation and sensitivity testing of M100 TB Broth Base Mycobacterium tuberculosis. For cultivation of Mycobacterium tuberculosis FD018* Middlebrook OADC Growth Supplement MV100 TB HiVeg™ Broth Base FD348* OADS Supplement For usage refer M100 M196 Middlebrook 7H10 Agar Base, Special M034 TB Broth Base w/o Tween 80 For isolation, cultivation and sensitivity testing of For cultivation of Mycobacteria when the presence Mycobacterium tuberculosis. of oleic acid is undesirable. FD018* Middlebrook OADC Growth Supplement MV034 TB HiVeg™ Broth Base w/o Tween 80 FD348* OADS Supplement For usage refer M034 M511 Middlebrook 7H11 Agar Base M1059 Wayne Sulphatase Agar Base For isolation, cultivation and sensitivity testing of For bio chemical differentiation of Mycobacteria on Mycobacteria. the basis of their ability to produce aryl sulphatase. FD018* Middlebrook OADC Growth Supplement FD348* OADS Supplement MV511 Middlebrook 7H11 HiVeg™ Agar Base For usage, supplement refer M511 M511A Middlebrook 7H11 Agar Base w/o Malachite Green For isolation, cultivation and determination of antimicrobial susceptibility of Mycobacteria. FD018* Middlebrook OADC Growth Supplement FD348* OADS Supplement Ready Prepared Media* L.J. Medium Slants with and without antibiotics MT001 Modified Middlebrook 7H9 Broth with Indicator SL067L L.J Medium Slant w/ Capreomycin (40 µg/ml) for cultivation of Mycobacterium tuberculosis. (long tube) SL001 L.J. Medium Slant for cultivation of Mycobacterium tuberculosis and for cultivation of Mycobacterium tuberculosis. for resistance testing. SL001T L.J. Medium Slant in thick glass bottles SL017 L.J.Medium Slant w/ D - Cycloserine (30 µg/ml) for cultivation of Mycobacterium tuberculosis. (long tube) for cultivation of Mycobacterium tuberculosis and SL179 L.J. Slopes for BCG Vaccines for resistance testing. for enumeration of BCG vaccines as per IP chapter 2.2.5. SL169L L.J.Medium Slant w/ Ethionamide (20 µg/ml) (long tube) SL022 L.J. Medium Slant w/o Glycerol for cultivation of Mycobacterium tuberculosis and for cultivation of Mycobacterium tuberculosis and for resistance testing. for resistance testing. SL170L L.J Medium Slant w/ Ethionamide (40 µg/ml) SL167 L.J. Medium slants w/ Augmentin (20 µg/ml) (long tube) for cultivation of Mycobacterium tuberculosis and for cultivation of Mycobacterium tuberculosis and for resistance testing. for resistance testing. On recept all the above products to be stored between 10-30°C *On receipt store between 2-8°C. Concentration marked in red are as per WHO recommendation. On request kit of assorted antibiotic slant as per requirement can be provided SL000L* The
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