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J. Bio. & Env. Sci. 2021 Journal of Biodiversity and Environmental Sciences (JBES) ISSN: 2220-6663 (Print) 2222-3045 (Online) Vol. 18, No. 6, p. 18-28, 2021 http://www.innspub.net RESEARCH PAPER OPEN ACCESS Identification and pharmacological properties of coastal plants from Barangay Diguisit, Baler, Aurora Zedrick A. Ventura1, Diana C. Castillo*2,3, Evaristo A. Abella2,3 1Department of Biological Sciences, College of Science, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines 2Faculty, Department of Biological Sciences, College of Science, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines 3Biodiversity Conservation Laboratory, Interactive Laboratory, Department of Biological Sciences, College of Science, Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines Article published on June 30, 2021 Key words: Coastal plants, Pharmacological properties, Antibacterial properties, Antioxidant activities Abstract This study was conducted to screen the pharmacological properties of the different coastal plants located along coastal areas of Diguisit, Baler, Aurora. Six coastal plants were collected and were identified as Dracaena fragrans, Pueraria montana var. lobata, Sphagneticola trilobata, Urticaceae, Nephrolepis cordifolia, and Pandanaceae. Ethanol extracts of plants were evaluated for antibacterial properties against S. aureus and E. coli using disc diffusion method. Positive results were revealed in S. aureus both in 24 and 48 hours as zones of inhibitions were manifested. S. trilobata and P. montana var. lobata has the largest zone of inhibition. On the other hand, only the P. montana var. lobata, S. trilobata, and Urticaceae showed positive result against E.coli at 24 hours. At the 48th hour of incubation, a decrease in zones of inhibition was observed. Antioxidant activity assay was evaluated in 2,2-diphenyl-1-picrylhydrazyl (DPPH), trolox equivalent. All of the coastal plants collected showed antioxidant activity; Pandanaceae and S. trilobata were recorded with the highest antioxidant activity. The results in test for antibacterial properties and antioxidant activities suggested that the coastal plants collected had the potential for pharmacological properties. *Corresponding Author: Diana C. Castillo [email protected] 18 | Ventura et al. J. Bio. & Env. Sci. 2021 Introduction leaf parts of the plants were collected by handpicking Higher plants have been described as chemical before assaying. The leaves were rinsed with water factories that are capable of synthesizing unlimited and placed in a clean, separate, and sealed plastic number of highly complex and unusual chemical bags until extraction process. substances. The World Health Organization (2004) estimated that 80% of the people in developing For the samples needed for authentication, vegetative countries of the world rely on traditional medicine for and reproductive part of the plants were collected and their primary health care needs, and about 85% of instantly washed with ethyl alcohol before placing in traditional medicine involves the use of plant extracts. clean plastic bags. This means that about 3.5 to 4 billion people in the world rely on plants as sources of drugs. Plant Extraction Pharmacology, by definition, is the branch Plant samples were cut into small pieces for air-drying of biology concerned with the study of drug action and were powderized. Ethanolic extraction and which exerts a biochemical or physiological effect on concentration using rotary evaporation was done in the the cell, tissue, organ, or organism (Vallance & Smart, Rice Chemistry and Food Science Division, Philippine 2006). If substances have medicinal properties, they Rice Research Institute, Nueva Ecija. The extracts are considered pharmaceuticals (Finkel et al., 2009). obtained were stored in a refrigerator until use. The Philippines is high in terms of plant diversity that Evaluation of Antibacterial Property an estimated 30-40% of these plants is endemic to the Test Microorganisms country and found nowhere else in the world The pure culture of the gram positive Staphylococcus (Amoroso, 2012). The municipality of Baler in Aurora aureus and the gram negative Escherichia coli is present with coastal areas. Beaches in Baler, Aurora bacteria were provided by the Department of were abundantly rearing with different coastal plants Biological Sciences, College of Arts and Sciences, growing along the shore and rock formations. Central Luzon State University. The Philippine Government RA No. 8423 or the Preparation of Bacterial Suspension “Traditional and Alternative Medicine Act (TAMA) of Ten milliliters of nutrient broth (NB) was prepared 1997” was declared and stated to improve the quality and dispensed into test tubes and sterilized on an and delivery of health care services to the Filipino autoclave at 121°C, 15 psi for 15 mins. A loopful of the people. One of the objectives is to promote and two bacteria was inoculated on the prepared nutrient advocate the use of traditional, alternative, preventive broth. The suspension was then incubated at sanitize and curative health care modalities that have been room temperature for 6 hours before adjusting the proven safe, effective, cost effective and consistent turbidity using 0.5mcFarland Turbidity Standard. with government standards on medical practice. This study of the different coastal plants located along the Preparation of Culture Media beaches of Barangay Diguisit, Baler, Aurora was The Mueller-Hinton Agar (MHA) was prepared based evaluated for its pharmacological properties through antibacterial properties and antioxidant analysis. on the instruction given. The MH agar powder was boiled in distilled water until totally dissolved in a Materials and methods flask. It was sterilized in an autoclave at 121°C, 15 psi Plant Materials for 15 mins, then cooled in a water bath at 45-50°C. Different coastal plants from Diguisit, Baler, Aurora Twenty milliliters of the prepared medium was were collected and authenticated. For the samples aseptically pour plated into sterilized petri plates in a needed for extraction, the healthy and undamaged clean and sanitize chamber. 19 | Ventura et al. J. Bio. & Env. Sci. 2021 Antibacterial Activity Assay Table 1. Collection site of the collected coastal plants. Antibacterial activity was tested by disc diffusion Collection Sites Treatments Total method in which sterilized 6mm filter paper disc was 1 2 3 4 5 Dracaena ✓ 2 ✓ used. About 100 µL of the bacteria was pipetted into fragrans Pueraria montana ✓ ✓ 3 the media and swabbed with sterile cotton swabs. ✓ var. lobata Furthermore, the filter paper discs were soaked with Sphagneticola 1 ✓ 0.01mL of the streptomycin, ethanol, and the trilobata Urticaceae ✓ ✓ 2 different ethanol plant extracts. Streptomycin and the Nephrolepis ✓ 1 ethanol solvent were used as the positive and negative cordifolia Pandanaceae ✓ 1 control, respectively. Plates were incubated at room temperature (37ºC) for 24 and 48 hours. Antioxidant Activity Assay The plant extract samples were analyzed in Rice Chemistry and Food Science Division, Philippine Rice Research Institute, Science City of Muñoz, Nueva Ecija, Philippines for the determination of their antioxidant properties in terms of DPPH Radical Scavenging Assay in trolox equivalent. Results and discussion Coastal Plant Collection Sites Fig. 1 showed the map of the coordinates of the collection sites of the coastal plants. Five 10 x 10 m Fig. 2. Collection sites of the coastal plants; (A) quadrat were laid in the area in the coast of Barangay Collection site 1, (B) Collection site 2, (C) Collection Diguisit, Baler, Aurora. Table 1 showed the coastal site 3, (D) Collection site 4, and (E) Collection site 5. plants and the collection sites where they can be found growing. Some of the plants can also be found Collection site 2 has a sandy-soil substratum wherein growing in other collection sites (Fig. 2). S. trilobata invades most of the area along with the Urticaceae that grows in the area. The 3rd collection site was an area of sand substratum with vastly growing Pandanaceae along with palms and trees. The 4th collection site was located near a waterfall Fig. 1. Map of the coordinates of the collection sites with large boulders of rocks. P. montana var. lobata of coastal plants; (A) Collection site 1, (B) Collection was found creeping in this area. It was a soil-rocky site 2, (C) Collection site 3, (D) Collection site 4, (E) substratum and located about 3 m from the water at Collection site 5. the coast. Collection site 1 was an area of a rocky-soil and sand Collection site 5 was located in a more elevated area. substratum located about 4 m from the water. D. The plants collected in this area were N. cordifolia, D. fragrans, P. montana var. lobata and Urticaceae fragrans, Pandanaceae, P. montana var. lobata, and were collected in this area. some trees. 20 | Ventura et al. J. Bio. & Env. Sci. 2021 Authentication of Coastal Plants Stems may reach up to 30cm diameter on old plants. A total of 6 coastal plants belonging to 6 families, 4 The leaves are glossy green, lanceolate, 20–150cm long genera, 4 species and 1 variety were collected and and 2–12cm wide; small leaves are erect to spreading, recorded from the coastal areas of Barangay Diguisit, and larger leaves usually drooping under their weight Baler, Aurora. These were Cornstalk Dracaena (Huxley, 1992). The flowers are produced (Dracaena fragrans), East Asian Arrowroot in panicles 15–160cm