DOCSLIB.ORG

Hillsborough County Florida

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Hillsborough County Florida HILLSBOROUGH COUNTY FLORIDA REQUEST FOR PROPOSALS FOR CARROLLWOOD FIRE STATION NO. 19 REPLACEMENT (CIP NO. C91183) SUNDANCE FIRE STATION NO. 8 EXPANSION & RENOVATION (CIP NO. C91187) & RIVERVIEW FIRE STATION NO. 16 REPLACEMENT (CIP NO. C91185) Volume 4 of 6 (Specifications for Sundance – Part 1 of 2) CONSTRUCTION MANAGER (CM) AT-RISK RFP NO. : RFP-C-0013-0-2015/DV CONTACT PERSON: DENISE VASQUEZ SR. PROCUREMENT ANALYST BUSINESS & SUPPORT SERVICES (PROCUREMENT) 601 E. KENNEDY BLVD., 18TH FLOOR P.O. BOX 1110 TAMPA, FLORIDA 33602 TELEPHONE: (813) 301-7088 FAX: (813) 272-6290 TABLE OF CONTENTS SUNDANCE FIRE STATION No. 8 EXPANSION & RENOVATION DIVISION 1 – GENERAL REQUIREMENTS 01010 SUMMARY OF WORK 01030 ALTERNATES/OPTIONS 01040 PROJECT COORDINATION 01045 CUTTING & PATCHING 01050 FIELD ENGINEERING 01070 ABBREVIATIONS 01095 REFERENCE STANDARDS AND DEFINITIONS 01200 MEETINGS & CONFERENCES 01300 CONSTRUCTION MANAGER SUBMITTALS 01310 BAR CHART SCHEDULE 01340 SHOP DRAWINGS, PRODUCT DATA AND SAMPLES 01400 QUALITY CONTROL 01410 MATERIALS TESTING 01500 TEMPORARY FACILITIES 01505 MOBILIZATION 01530 BARRICADES 01560 TEMPORARY ENVIRONMENTAL CONTROLS 01600 MATERIALS AND EQUIPMENT 01610 FLORIDA PRODUCT APPROVAL REQUIREMENTS 01631 SUBSTITUTIONS 01700 PROJECT CLOSE-OUT 01710 FINAL CLEANING 01720 PROJECT RECORD DOCUMENTS 01730 OPERATING AND MAINTENANCE DATA 01740 WARRANTIES DIVISION 2 – SITEWORK 02070 SITE DEMOLITION 02110 SITE CLEARING 02300 EARTHWORK 02361 TERMITE CONTROL 02480 LANDSCAPE WORK 02513 ASPHALT CONCRETE PAVING 02666 POTABLE WATER SYSTEMS 02668 FIRE WATER SYSTEMS 02670 WATER WELL SYSTEMS 02720 STORM SEWAGE SYSTEMS 02730 SANITARY SEWAGE SYSTEMS 02740 SEPTIC SYSTEMS 02810 UNDERGROUND IRRIGATION SYSTEM 02821 CHAIN-LINK FENCES 02825 VINYL FENCING DIVISION 3 – CONCRETE 03300 CAST-IN-PLACE CONCRETE DIVISION 4 – MASONARY SUNDANCE FIRE STATION NO. 8 EXPANSION & RENOVATION TABLE OF CONTENTS CIP NO. 91187 TOC - 1 04810 UNIT MASONRY ASSEMBLIES DIVISION 5 – METALS NOT USED DIVISION 6 – WOOD AND PLASTICS 06100 ROUGH CARPENTRY 06176 METAL-PLATE-CONNECTED WOOD TRUSSES 06201 EXTERIOR FINISH CARPENTRY 06410 PLASTIC LAMINATE CASEWORK DIVISION 7 – THERMAL AND MOISTURE PROTECTION 07180 POLYURETHANE DECK COATING 07210 BUILDING INSULATION 07411 METAL ROOF PANELS 07620 SHEET METAL FLASHING AND TRIM 07841 FIRESTOP SYSTEMS 07920 JOINT SEALANTS DIVISION 8 – DOORS AND WINDOWS 08111 STANDARD STEEL DOORS AND FRAMES 08211 FLUSH WOOD DOORS 08361 SECTIONAL OVERHEAD DOORS 08410 ALUMINUM ENTRANCES AND STOREFRONTS 08520 ALUMINUM WINDOWS 08710 DOOR HARDWARE 08800 GLAZING DIVISION 9 – FINISHES 09220 PORTLAND CEMENT PLASTER 09260 GYPSUM BOARD ASSEMBLIES 09310 CERAMIC TILE 09511 ACOUSTICAL PANEL CEILINGS 09650 RESILIENT FLOORING 09653 RESILIENT WALL BASE AND ACCESSORIES 09900 PAINTING DIVISION 10 – SPECIALTIES 10200 LOUVERS AND VENTS 10350 FLAGPOLES 10431 SIGNS 10522 FIRE EXTINGUISHERS, CABINETS AND ACCESSORIES 10800 TOILET AND BATH ACCESSORIES DIVISION 11 – EQUIPMENT 11451 RESIDENTIAL APPLIANCES SUNDANCE FIRE STATION NO. 8 EXPANSION & RENOVATION TABLE OF CONTENTS CIP NO. 91187 TOC - 2 DIVISION 12 – FURNISHINGS 12491 HORIZONTAL LOUVER BLINDS DIVISION 13 – SPECIAL CONSTRUCTION NOT USED DIVISION 14 – CONVEYING SYSTEMS NOT USED DIVISION 21 – FIRE PROTECTION 21 00 00 FIRE SUPPRESSION GENERAL REQUIREMENTS 21 01 00 PROJECT CLOSE OUT 21 02 00 OPERATIONS AND MAINTENANCE MANUALS 21 05 00 ELECTRICAL POWER AND AUXILIARIES 21 08 00 PAINTING AND IDENTIFICATION 21 10 00 FIRE SUPPRESSION SYSTEM 21 15 00 FIRE PUMPS 21 20 00 FIRE SUPPRESSION SYSTEM SPECIALTIES DIVISION 22 – PLUMBING 22 00 00 PLUMBING GENERAL REQUIREMENTS 22 01 00 PROJECT CLOSE OUT 22 02 00 OPERATIONS AND MAINTENANCE MANUALS 22 05 00 ELECTRICAL POWER AND AUXILIARIES 22 08 00 PAINTING AND IDENTIFICATION 22 10 00 PLUMBING – GENERAL 22 15 00 ELECTRIC WATER HEATERS 22 20 00 PIPING GENERAL 22 21 00 PIPING 22 51 00 INSULATION DIVISION 23 – MECHANICAL 23 00 00 MECHANICAL (HVAC) GENERAL REQUIREMENTS 23 01 00 PROJECT CLOSE OUT 23 02 00 OPERATIONS AND MAINTENANCE MANUALS 23 05 00 ELECTRICAL POWER AND AUXILIARIES 23 08 00 PAINTING AND IDENTIFICATION 23 20 00 PIPING GENERAL 23 22 00 REFRIGERANT PIPING 23 24 00 CONDENSATE DRAIN PIPING 23 41 00 CORROSION PROTECTION 23 42 00 DIRECT EXPANSION (DX) HVAC EQUIP. 23 43 00 DIR EXP (DX) HVAC EQUIP.-100% OUTDOOR AIR UNITS 23 65 00 INSULATION 23 70 00 FANS AND VENTS 23 73 00 AIR DISTRIBUTION SYSTEMS 23 73 01 SPECIAL DUCT SYSTEMS 23 95 00 TEST AND BALANCE SUNDANCE FIRE STATION NO. 8 EXPANSION & RENOVATION TABLE OF CONTENTS CIP NO. 91187 TOC - 3 DIVISION 26 – ELECTRICAL 26 01 00 BASIC ELECTRICAL REQUIREMENTS 26 05 19 BUILDING WIRE AND CABLE 26 05 26 GROUNDING AND BONDING 26 05 29 HANGERS AND SUPPORTS 26 05 33 RACEWAYS AND BOXES 26 12 00 BUILDING WIRE AND CABLE 26 24 19 LOAD CENTERS 26 27 26 WIRING DEVICES 26 28 16 DISCONNECT SWITCHES 26 43 13 SURGE SUPPRESSION (SPD) 26 51 00 INTERIOR LIGHTING SCHEDULE A – REPORT OF THE SUBSURFACE EXPLORATION AND GEOTECHNICAL EVALUATION SERVICES SCHEDULE B – REPORT OF NESHAP ASBESTOS AND LEAD – BASED PAINT SURVEY END OF TABLE OF CONTENTS SUNDANCE FIRE STATION NO. 8 EXPANSION & RENOVATION TABLE OF CONTENTS CIP NO. 91187 TOC - 4 SECTION 01010 – SUMMARY OF WORK PART 1 - GENERAL 1.1 RELATED DOCUMENTS Drawings and general provisions of the Contract, including General and Special Conditions, other Division-1 Specification Sections, and the CM-AT Risk GMP RFP Package “RFP” apply to this Section. 1.2 SUMMARY OF CONSTRUCTION MANAGER’S (CM’S) SCOPE OF WORK The Summary of CM’S Scope of Work is generally described herein : In general, the name of the Project is the “Sundance Fire Station Expansion & Renovation”. The Work includes but is not limited to the furnishing of all labor, materials, equipment, permitting services, field engineering, construction management, and project administration/supervision activities to construct fully finished and functional fire station. The facility must be of high quality and low maintenance; must meet or exceed the requirements of the GMP Construction Documents Package and must meet the needs of the user and maintenance agencies. The construction of this facility shall meet the requirements of all governing local, state and federal regulations, codes and ordinances, including the Florida Accessibility Code for Building Construction and the Americans with Disabilities Act (ADA). 1.1.2 The Location information for the Project is as follows: Sundance Fire Station No. 8 Expansion & Renovation (CIP No 91187): 602 Lightfoot Road, Wimauma, FL 33547 Generally the construction work includes, but is not limited to: selective demolition work, approximately 2,400 sq. ft. of building expansion and approximately 3,000 sq. ft. of building renovation, together with associated on-site and off-site improvements such as such as access drives, parking lot, sidewalks, new septic system, dumpster enclosure, flagpole, landscaping, irrigation, storm drainage system, fence ,emergency generator and all necessary utilities including a fire suppression system (NFPA 13 System), fire alarm system, 911 “Tear & Go “ system & security surveillance system. A. In general, the Work includes but is not limited to the furnishing of all labor, materials, equipment, permitting services, field engineering, construction management, and project administration/supervision activities to construct a fully finished and functional facility. The facility must be of high quality and low maintenance; must meet or exceed the requirements of the GMP Construction Documents Package and must meet the needs of the user and maintenance agencies. The construction of the facility shall meet the requirements of all governing local, state and federal regulations, codes and ordinances, including the Florida Accessibility Code for Building Construction and the Americans with Disabilities Act (ADA). B. The Construction Documents also include certain Alternates and Options which may or may not be included as part of the Work at the COUNTY’S option. These alternates and options are listed in Section 01030 Alternates and Options of Division One Specifications. 1.3. CONSTRUCTION MANAGER’S WORK The CM’S construction Work shall include, but shall not be limited to the furnishing of all labor, materials, equipment, and project administration/supervision activities, including scheduling, to construct one fully finished facility which meets or exceeds the program and standards set forth in the RFP as follows: A. Obtaining all permits and approvals from regulatory and utility agencies. SUNDANCE FIRE STATION NO.8 EXPANSION & RENOVATION SUMMARY CIP NO. 91187 01010 - 1 B. All required on-site and off-site work associated with the project including but not limited to access drives, sidewalks, parking, signage, flagpole, refueling facility, landscaping and irrigation, water and wastewater connections and extensions, electrical utility services, fire protection, storm water retention system, etc. C. Construction of one complete, functional fire station facility as described in the GMP documents. D. The work includes all associated equipment and building systems as described in the GMP drawings and specifications including mechanical systems, water and sanitary systems, fire protection, electrical systems, conduit and wiring for data/telecommunications, emergency generator, 911 call system , etc. E. Provision of all close-out documents, including but not limited to as-built surveys and drawings, maintenance manuals, warranties and guarantees as described in Division 1 of the Specifications, Section 01700 “Project
Load more

Recommended publications
  • Northwest Region EMS Protocols
    Northwest Region EMS Protocols Clallam, Mason, Kitsap & Jefferson Counties and Navy Region NW MPD Approved December 2014 Washington State DOH approved October 2014 1 2014 - Northwest Region Emergency Medical Services & Trauma Care Council 2 2014 - Northwest Region Emergency Medical Services & Trauma Care Council Sections are color coded as follows: Introduction Regional Guidelines ...................................................................................................................... 8 NW Region Patient Care Procedures ............................................................................................ 8 CDC National Trauma Triage Procedure ....................................................................................... 9 Clallam .................................................................................................................................... 10-A Jefferson ................................................................................................................................. 10-B Mason ..................................................................................................................................... 10-C Kitsap ...................................................................................................................................... 10-D West Olympic Penninsula ....................................................................................................... 10-E Prehospital Provider Conduct .....................................................................................................
    [Show full text]
  • Bill's Cave Diving Lexicon
    Bill’s Cave Diving Lexicon 120 Rule: Noticing from the Navy NDL table that, for certain depths, depth + bottom time = 120 so that the NDL can be determined by subtracting the depth from 120. 200 DIN: Thread depth in a DIN valve and associated pressure (200 BAR) that can be handled. This size (7 threads) allows for a DIN to yoke conversion. 300 DIN: Thread depth in a DIN valve that provides the most secure (9 threads) connection and can withstand 300 BAR pressure. 5 nines pure: 99.999% pure, as in a gas. 50-50: Gas mix of 50% oxygen and 50% nitrogen used for decompression gas. 6351-T6 Aluminum Alloy: Alloy that has had problems with tank ruptures. Absolute Pressure: Total pressure being exerted on a diver At sea level Absolute pressure is 1 ATA and it increases by 1 ATA for each 33fsw (34ffw). ADDD (Air, Duration, Depth, Distance): Limits for dive termination acronym minimum Air volume/pressure, maximum Duration of dive, maximum Depth of dive, and maximum Distance of penetration. ADV (Automatic Deflation Valve, and Automatic Diluent Valve ): Device on a buoyancy compensator that allows for rapid air purging, and device on a rebreather that dilutes the breathing mix. AGE (Arterial Gas Embolism): A lung expansion injury. A condition in which gas bubbles enter the arterial system and cause damage by blocking blood flow to vital organs, most commonly the brain. This is generally caused by air passing through the walls of the alveoli into the bloodstream. Air: A gas mixture of Oxygen (21%), Nitrogen (78%), and other gasses (1%, Helium, Argon, etc.).
    [Show full text]
  • The Impact of the USS FORRESTAL's 1967 Fire on United States Navy Shipboard Damage Control
    THE IMPACT OF THE USS FORRESTAL’S 1967 FIRE ON UNITED STATES NAVY SHIPBOARD DAMAGE CONTROL A thesis presented to the Faculty of the U.S. Army Command and General Staff College in partial fulfillment of the requirements for the degree MASTER OF MILITARY ART AND SCIENCE Military History by HENRY P. STEWART, LCDR, USN B.S., Maine Maritime Academy, Castine, ME, 1992 M.S., Naval Postgraduate School, Monterey, CA, 1999 Fort Leavenworth, Kansas 2004 Approved for public release; distribution is unlimited. MASTER OF MILITARY ART AND SCIENCE THESIS APPROVAL PAGE Name of Candidate: LCDR Henry P. Stewart, USN Thesis Title: The Impact of the USS Forrestal’s 1967 Fire on United States Navy Shipboard Damage Control Approved by: , Thesis Committee Chair LTC Marian E. Vlasak, M.A. , Member CDR David Christie, M.M.A.S. , Member Jerold E. Brown, Ph.D. Accepted this 18th day of June 2004 by: , Director, Graduate Degree Programs Robert F. Baumann, Ph.D. The opinions and conclusions expressed herein are those of the student author and do not necessarily represent the views of the U.S. Army Command and General Staff College or any other governmental agency. (References to this study should include the foregoing statement.) ii ABSTRACT THE IMPACT OF THE USS FORRESTAL’S 1967 FIRE ON UNITED STATES NAVY SHIPBOARD DAMAGE CONTROL, by LCDR Henry P. Stewart, United States Navy, 112 pages. This thesis examines the impact of the 1967 flight deck fire on the aircraft carrier USS Forrestal (CVA 59) and the resulting two investigations, on the development of US Navy damage control doctrine and equipment.
    [Show full text]
  • Biomarkers of Breast Cancer Cell Lines A; Pilot Study on Human Breast Cancer Metabolomics
    Article ID: WMC004092 ISSN 2046-1690 Biomarkers of Breast Cancer Cell Lines A; Pilot Study on Human Breast Cancer Metabolomics Corresponding Author: Dr. Sumaira N Syed, Clinical Fellow, General Surgery, Bedford Hospital, 72midland road, MK40 1QH - United Kingdom Submitting Author: Dr. Sumaira N Syed, Clinical Fellow, General Surgery, Bedford Hospital, 72midland road, MK40 1QH - United Kingdom Article ID: WMC004092 Article Type: Research articles Submitted on:05-Mar-2013, 10:04:10 AM GMT Published on: 05-Mar-2013, 12:32:39 PM GMT Article URL: http://www.webmedcentral.com/article_view/4092 Subject Categories:BREAST Keywords:Breast Cancer, Biomarkers, Metabolomics How to cite the article:Syed SN. Biomarkers of Breast Cancer Cell Lines A; Pilot Study on Human Breast Cancer Metabolomics . WebmedCentral BREAST 2013;4(3):WMC004092 Copyright: This is an open-access article distributed under the terms of the Creative Commons Attribution License(CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Source(s) of Funding: None Competing Interests: None WebmedCentral > Research articles Page 1 of 25 WMC004092 Downloaded from http://www.webmedcentral.com on 06-Mar-2013, 10:36:07 AM Biomarkers of Breast Cancer Cell Lines A; Pilot Study on Human Breast Cancer Metabolomics Author(s): Syed SN Abstract metabolism of tumour cells(3). Although it has been known for quite a long time now that tumour cell metabolism is different from the metabolism of normal cell, the altered metabolites of breast cancer cells Metabolism of a cancer cell is significantly different have not yet been utilized as metabolic biomarkers for from that of a normal cell.
    [Show full text]
  • Structure and Metabolism of the Intracellular Polysaccharide of Corynebacterium. Don D
    Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 1969 Structure and Metabolism of the Intracellular Polysaccharide of Corynebacterium. Don D. Mickey Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Mickey, Don D., "Structure and Metabolism of the Intracellular Polysaccharide of Corynebacterium." (1969). LSU Historical Dissertations and Theses. 1679. https://digitalcommons.lsu.edu/gradschool_disstheses/1679 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. This dissertation has been microfilmed exactly as received 70-9079 MICKEY, Don D., 1940- STRUCTURE AND METABOLISM OF THE INTRACELLULAR POLYSACCHARIDE OF CORYNEBACTERIUM. The Louisiana State University and Agricultural and Mechanical College, Ph.D., 1969 Microbiology University Microfilms, Inc., Ann Arbor, Michigan STRUCTURE AND METABOLISM OF THE INTRACELLULAR POLYSACCHARIDE OF CORYNEBACTERIUM A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The Department of Microbiology by Don D. Mickey B. S., Louisiana State University, 1963 August, 1969 ACKNOWLEDGMENT The author wishes to acknowledge Dr. M. D. Socolofsky for his guidance during the preparation of this dissertation. He also wishes to thank Dr. H. D. Braymer and Dr. A. D. Larson and other members of the Department of Microbiology for helpful advice given during various phases of this research.
    [Show full text]
  • Price List- 4W
    PRICE LIST- 4W Price Revision New Introduction With Effect From 01 Mar 2019 LIGHTING S.no HSN Item Code SAP Code Description MRP Std. Pkg MOQ MSZ 800 CC CAR Head Lamp 1 85122010 031-HLA- - 61001695 H/L ASSY 800 CC T -1 330 16 16 2 85122010 031-HLA-MN 61005810 HL ASSY 800CC CAR MFR P43 W/O 'P' 517 24 12 3 85122010 031-HLA-PN - 61001696 H/L ASSY. N/M MARUTI 800CC CAR (P43T) 356 16 8 4 85122010 030-HLU-T - 61001668 H/L UNIT 800CC P45T WT THERMOCOL 198 24 12 5 85122010 030-HLU-MN - 61001663 REF. UNIT M 800CC CAR MFR P43T W/O PARKI 378 24 12 6 85122010 030-HLU-N - 61001665 R/UNIT MARUTI 800CC CAR(P43T) 201 24 12 7 85122010 030-HLU-PA - 61001666 REF. UNIT M.CAR 800CC WITH PARKING 208 24 12 8 85122010 030-HLU-PNA- 61001667 REFLECTOR UNIT M 800CC CAR P43T WITH PAR 208 24 12 9 85122010 029-HLU-L - 61001637 H/L UNIT CAR 800CC Y5A LH 411 18 18 10 85122010 029-HLU-R - 61001642 H/L UNIT CAR 800CC Y5A RH 411 18 18 11 85122010 029-HLU-LL - 61001638 H/L UNIT CAR 800CC Y5A LASER LH 314 18 9 12 85122010 029-HLU-LR - 61001639 H/L UNIT CAR 800CC Y5A LASER RH 314 18 9 13 85122010 029-HLU-NWM-BK-L 61006171 H/L UNIT 800CC CAR T-3 WT BK HSG LH 914 12 12 14 85122010 029-HLU-NWM-BK-R 61006172 H/L UNIT CAR 800CC T3 WT BK HSG RH 914 12 12 15 85122010 029-HLU-NWM-L 61001640 H/L UNIT 800CC T-3 MFR LH 903 12 12 16 85122010 029-HLU-NWM-R 61001641 H/L UNIT 800CC T-3 MFR RH 903 12 12 17 85122010 030-HLU-MNP- 61001664 H/L UNIT 800CC MFR P43T WT PARKING 390 24 12 18 85122010 031-HLA-MNP 61005811 H/L ASSY 800CC N/M MFR P43T WT PARKING 529 24 12 Tail Lamps 19 85122010 031-RCU-L
    [Show full text]
  • American Academy of Underwater Sciences (AAUS) Standards For
    The American Academy of Underwater Sciences STANDARDS FOR SCIENTIFIC DIVING AAUS • 101 Bienville Blvd Dauphin Island, AL 36528 www.aaus.org • [email protected] • 251.591.3775 FOREWORD Since 1951 the scientific diving community has endeavored to promote safe, effective diving through self- imposed diver training and education programs. Over the years, manuals for diving safety have been circulated between organizations, revised and modified for local implementation, and have resulted in an enviable safety record. This document represents the minimal safety standards for scientific diving at the present day. As diving science progresses so shall this standard, and it is the responsibility of every member of the Academy to see that it always reflects state of the art, safe diving practice. American Academy of Underwater Sciences ACKNOWLEDGEMENTS The Academy thanks the numerous dedicated individual and organizational members for their contributions and editorial comments in the production of these standards. Revision History April, 1987 October, 1990 May, 1994 January, 1996 March 1999 Added Sec 7.6.1 Nitrox Diving Guidelines. Revised Appendix 7 and 11. January 2001 Revised Section 1.23.1 DSO Qualifications. Revised Section 5.31.4 Emergency Care Training. Revised Section 6 Medical Standards. Made Sec 7.6.1 Nitrox Diving Guidelines into Section 7. Added Section 8.0 Scientific Aquarium Diving. Moved Section 7.0 to Section 9.0 Other Diving Technologies. April 2002 Removed Appendix 7 AAUS Checkout Dive and Training Evaluation. Revised Section 5.33.3. Revised Section 4.23.2. August 2003 Section 1.27.3 Delete reference to Appendix 9 (checkout dive). Section 1.4 Remove word "waiver".
    [Show full text]
  • A Deeper Understanding of Intestinal Organoid Metabolism Revealed by Combining Fluorescence Lifetime Imaging Microscopy (FLIM) and Extracellular Flux Analyses
    bioRxiv preprint doi: https://doi.org/10.1101/771188; this version posted September 16, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. A deeper understanding of intestinal organoid metabolism revealed by combining fluorescence lifetime imaging microscopy (FLIM) and extracellular flux analyses Irina A. Okkelman1, Nuno Neto2,3, Dmitri B. Papkovsky1, Michael Monaghan2,3,4, Ruslan I. Dmitriev1,5 1Laboratory of Biophysics and Bioanalysis, ABCRF, University College Cork, Cavanagh Pharmacy Building, College Road, Cork, T12 K8AF, Ireland 2Department of Mechanical and Manufacturing Engineering, Trinity College Dublin, Ireland 3Trinity Centre for Biomedical Engineering, Trinity College Dublin, Ireland 4Advanced Materials and BioEngineering Research (AMBER) Centre at Trinity College Dublin and Royal College of Surgeons in Ireland, Dublin, Ireland. 5Institute for Regenerative Medicine, I.M. Sechenov First Moscow State University, Moscow, Russian Federation Abstract Stem cells and the niche in which they reside feature a complex microenvironment with tightly regulated homeostasis, cell-cell interactions and dynamic regulation of metabolism. A significant number of organoid models has been described over the last decade, yet few methodologies can enable single cell level resolution analysis of the stem cell niche metabolic demands, in real-time and without perturbing integrity. Here, we studied the redox metabolism of Lgr5-GFP intestinal organoids by two emerging microscopy approaches based on luminescence lifetime measurement – fluorescence- based FLIM for NAD(P)H, and phosphorescence-based PLIM for real-time oxygenation.
    [Show full text]
  • Standards and Procedures
    TM Standards and Procedures Version 3.2a January 2013 UTD International, LLC 11211 Sorrento Valley Road, Suite i San Diego, CA 92121 [email protected] www.unifiedteamdiving.com +1 760-585-9676 © 2013 UTD International, LLC UTD International, LLC • Standards and Procedures v3.1 Table of Contents 1.0 General ....................................................................................................1 1.1 Overview .................................................................................................1 Mission ...............................................................................................1 Ethos ..................................................................................................1 UTD Covenants .................................................................................2 Certification Philosophy .....................................................................2 Teaching Philosophies .......................................................................3 1.2 Core Teaching Principles .........................................................................3 Training Steps ....................................................................................4 1.3 Training Process and Definitions .............................................................5 Definitions Relevant to Standards and Procedures ...........................6 Evaluation ..........................................................................................7 UTD Diver Certification ......................................................................7
    [Show full text]
  • Volume 12 Number 3, June 1985
    ........ ( I . __ ._-----_._---_._-- ',',,',,', ,h Vol. 12, No.3 2 UNDERWATER SPELEOLOGY - PROGRAM COORDINATORS - CAVE DIVING SECTION OF THE NATIONAL SPELEOLOGICAL SOCIETY, INC. Annual Sci/Tech Journal: WAYNE MARSHALL _ BOARD OF DIRECTORS - Winter Workshop MARK LONG Co-Chairmen: WES SKILES Chairman: STEVE ORMEROID 629 West 4th St. Safety Coordinator & MARK LEONARD Marysville, OH 43040 Abe Davis Awards: Rt. 14, Box 136 (513) 642-7775 Lake City, FL 32055 (904) 752-1087 Vice-Chairman: HARK LONG Accident Investigation HENRY NICHOLSON P. O. Box 1633 & Recovery Team: 4517 Park St. Leesburg, FL 32749-1633 Jacksonville, FL 32205 (904) 787-5627 (904) 384-2818 Accident Files: WES SKILES Secretary­ JOE PROSSER Treasurer: (for Section business) Scientific Investiga­ BILL FEHRING P. O. Box 950 tion & Conservation: 3508 Hollow Oak Place Branford, FL 32008-0950 Brandon, FL 33511 (personal) (813) 689-7520 7400 N.W. 55th St. Publications: H.V. GREY Miami, FL 33166 (305) 592-3146 WORKSHOP: Dec. 28-29, Branford, FL. Theme: "Science Training Chairman: WES SKILES and Cave Diving." More information in future issues. P. O. Box 73 Branford, FL 32008 (904) 935-2469 CHAIRMAN'S MESSAGE Two years ago, I attended my first NSS Convention, Directors: WAYNE MARSHALL to represent the CDS. I had been forewarned that P. o. Box 1414 the cave divers were looked upon as a strange group Seffner, FL33584 of individuals who called themselves cavers. My ,,:JEFF BOZANIC (813) 681-3629 personal acquaintance with dry cavers, up- to that If" ,.'~P._ O. Box 490462 time, was with the few members of the CDS who were \1 "T-Key Biscayne, FL DALE-PURCHASE active dry cavers and those that I had met the week .
    [Show full text]
  • Diving Safety Manual
    North Carolina State University Diving Safety Manual Written and Complied by The Diving Control Board NC State University February 2007 Acknowledgements This manual includes the standards embodied in the AAUS 1996 Guidelines for Conduct of Scientific Diving Programs and covers the local standard of practice as per the needs of the NC State University. This document was authored and edited by members of the NC State University Diving Control Board: Larry Brown (Diving Safety Officer), David Eggleston, Thomas Wolcott, Gayle Plaia, and Ken Kretchman. Recognition and appreciation are expressed to David Pence (DSO at the University of Hawaii), Steve Sellers (Director of Diving & Water Safety at East Carolina University), and Doug Kesling (Training and Safety Coordinator for the National Undersea Research Center at the University of North Carolina at Wilmington) for their guidance in the preparation of this manual. Appreciation is also expressed to James Wagner, Zane Purvis, and Zeb Kaylor of N.C. State University and Gene Hobbs of Duke University for reviewing this manual. This document is based on, and is a modification of: The American Academy of Underwater Sciences STANDARDS FOR SCIENTIFIC DIVING AAUS • 430 Nahant Road, Nahant MA 01908-1696 NCSU Dive Safety Manual 2 FOREWORD Since 1951 the scientific diving community has endeavored to promote safe, effective diving through self-imposed diver training and education programs. Over the years, manuals for diving safety have been circulated between organizations, revised and modified for local implementation, and have resulted in an enviable safety record. This document represents the minimal safety standards for scientific diving at the present day.
    [Show full text]
  • Annual Report of the Town of Troy, New Hampshire
    Annual Reports of the Town Officers of TROY NEW HAMPSHIRE for the Year Ending December 31, 1961 and School District Officers for the Year Ending June 30, 1961 Digitized by the Internet Archive in 2013 http://archive.org/details/annualreportofto1961troy ANNUAL REPORTS OF THE Town Officers OF TROY FOR THE YEAR ENDING DECEMBER 31, 1961 SENTINEL PRINTING COMPANY, INC. KEENE, N. H. 1962 NH TOWN OFFICERS Moderator JAMES R. BAKER Town Clerk OLIVE M. TURLINGTON Treasurer ROGER C. SMITH Tax Collector OLIVE M. TURLINGTON Selectmen CLARENCE J. ABARE Term expires 1962 L. E. RAY HALE Term expires 1963 A. ARTHUR McKEW, JR. Term expires 1964 Overseer of Public Welfare ARTHUR W. BOWERS Representative ROBERT H. CONGDON Supervisors HAROLD R. LANG CHESTER E. PRIEST EMILE V. ROCHELEAU, SR. Auditors HAROLD E. BOYDEN F. TOM BUTLER Firewards ROBERT H. CONGDON Term expires 1962 GEORGE K. RIPLEY Term expires 1963 CHARLES A. LEPISTO Term expires 1964 Health Officer SAMUEL E. PAUL, M.D. Cemetery Sexton JOHN E. KAUPPINEN Cemetery Committee HERMAN K. SCHIERIOTH Term expires 1962 L. E. RAY HALE Term expires 1968 WILLIAM A. PARKER Term expires 1964 Highway Agent HENRY E. LAMPINEN Chief of Police JOHN W. KOSKI Town Hall Agent JOHN E. KAUPPINEN Water Commissioners EDGAR BROWN Term expires 1962 WILLIAM A. PARKER Term expires 1963 WILLIAM A. MATSON Term expires 1964 Trustees of Trust Funds ARTHUR W. BOWERS Term expires 1962 GEORGE K. RIPLEY Term expires 1963 A. ARTHUR McKEW, JR. Term expires 1964 Trustees of Public Library ALAN WHEELER Term expires 1962 A. ARTHUR McKEW, JR. Term expires 1963 MARGARET W.
    [Show full text]