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Table S1A. Patients and Tumor Characteristics of the Pdxs

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Table S1A. Patients and tumor characteristics of the PDXs TUMOR ID Passage Histology Gender Age Smoking Pack/Year Grade Stage TNM PDX1 2 LSCC Male 63 CURRENT 45 G4 (POOR) IIB pT3pN0 PDX2 2 LCNEC Male 71 FORMER 50 G4 (POOR) IIIA pT3pN1 PDX3 2 LSCC Male 76 FORMER 45 G2 (MOD.) IB pT2apN0 PDX4 2 LUAD Male 54 FORMER 85 G4 (POOR) IIB pT3pN0 PDX5 2 LSCC Male 74 CURRENT 50 G3 (POOR) IB pT2apN0 PDX6 2 LSCC Male 70 FORMER 40 G2 (MOD.) IIIA pT4pN1 PDX7 2 LUAD (ACINAR+SOLID) Male 52 CURRENT 37 na IIIA pT3N2 PDX8 2 LSCC Male 74 FORMER na na IIB pT2pN1 PDX9 48 LUAD Female 73 FORMER 20 G3 (POOR) IIIA T1aN2M0 PDX10 17 LUAD Male 74 CURRENT 120 G3 (POOR) IIIA T2bN2M0 PDX11 3 LUAD Male 67 FORMER 30 G3 (POOR) IA T1aN0M0 PDX12 3 LUAD Male 68 CURRENT 50 na IIIA T3N2M0 PDX13 1 LUAD (SOLID+MUCIN) Male 61 FORMER 23 G2 (MOD.) na pT2aNxMx PDX14 1 LSCC Male 61 CURRENT 45 G2 (MOD.) IA pT1aN0Mx LCNEC, lung neuroendocrine carcinoma LUAD, lung adenocarcinoma; LSCC, lung squamous cell carcinoma, na, not available Table 1SB. Exome sequencing depth and coverage report Sample Sample Million Yield % uniquely % Mean Coverage Coverage % of mouse Reference genome barcode name read-pairs (Gb) mapping unmapped coverage at Depth at Depth reads AB0115 N1 27,394 5,533 hsapiens.hs37d5 91,98 0,14 60,17 92,10 87,50 0,66 AB0117 PDX1 24,480 4,945 human.37 92,16 0,48 51,57 90,00 84,00 618N N2 23,458 6,01 hsapiens.hs37d5 92,44 0,16 50,00 88,77 75,35 0,87 AB0106 PDX2 20,858 4,213 human.37 92,64 0,29 48,52 90,60 54,60 AB0107 N3 27,219 5,498 hsapiens.hs37d5 92,81 0,16 60,35 94,90 91,30 1,01 AB0108 PDX3 27,119 5,478 human.37 91,88 0,31 57,82 90,50 85,30 AB0109 N4 26,767 5,407 hsapiens.hs37d5 92,70 0,18 59,22 94,10 90,20 0,95 AB0110 PDX4 26,972 5,448 human.37 91,59 0,47 55,56 90,70 85,10 P242 N5 45,868 9,265 human.37 92,76 0,21 113,96 92,72 89,09 2,09 P243 PDX5 39,085 7,895 human.37 93,06 0,23 100,72 89,27 84,53 P248 N6 45,485 9,188 human.37 93,83 0,17 116,54 93,30 89,82 1,52 P245 PDX6 47,442 9,583 human.37 94,59 0,18 124,88 92,46 88,87 P246 N7 39,526 7,984 human.37 94,09 0,16 100,83 92,21 88,34 9,38 P247 PDX7 42,645 8,614 human.37 91,31 0,40 108,43 89,49 84,90 P249 N8 33,175 6,701 human.37 90,53 2,28 74,92 93,59 89,79 3,24 P250 PDX8 28,646 5,787 human.37 90,18 2,45 65,70 89,79 84,33 T024 N9 41,276 8,338 human.37 90,64 2,51 95,15 95,87 93,28 8,77 T025 PDX9 31,221 6,307 human.37 89,52 2,44 73,22 92,86 88,42 T028 N10 42,286 8,542 human.37 89,97 1,89 100,80 95,95 93,43 3,62 T029 PDX10 33,008 6,668 human.37 89,84 1,85 76,51 92,46 88,09 T016 N11 31,586 6,380 human.37 90,95 2,17 71,95 93,38 89,39 2,54 T017 PDX11 34,195 6,907 human.37 90,79 2,59 81,03 93,58 89,79 T022 N12 37,195 7,634 human.37 90,87 2,21 86,42 95,18 92,12 2,47 T023 PDX12 38,892 7,856 human.37 89,99 2,01 92,34 94,98 91,91 AB0111 N13 27,311 5,517 hsapiens.hs37d5 92,49 0,24 62,84 94,50 91,10 3,49 AB0112 PDX13 26,784 5,410 human.37 88,57 1,36 53,03 89,80 83,50 AB0113 N14 27,263 5,507 hsapiens.hs37d5 92,63 0,30 61,21 94,30 90,60 2,14 AB0114 PDX14 27,764 5,608 human.37 89,76 0,91 56,50 90,50 85,10 RNA-seq report Million read- Sample Sample pairs (or Yield (Gb) barcode name reads) 617x PDX1 25,156 3,214 618x PDX2 30,357 5,136 619x PDX3 26,823 3,473 620x PDX4 44,079 6,611 U440 PDX5 32,536 4,945 U441 PDX6 39,503 6,004 U442 PDX7 43,750 6,650 U443 PDX8 32,358 4,918 U446 PDX10 33,923 5,156 U447 PDX11 33,819 5,141 Table S1D. Selection of regions and the genes with high levels of gene amplification ID PEAK REGION GENES WITHIN THE REGIONS WITH GA CANDIDATES OVEREXPRESED C2orf61, CALM2, EPCAM, MSH2, KCNK12, AC138655.1, MSH6, CALM2, EPCAM, MSH2, KCNK12, PDX3 chr2: 47202112-48822441 FBXO11,FOXN2, PPP1R21, AC073082.1, STON1_1,STON1-GTF2A1L MSH6, FBXO11, FOXN2, PPP1R21 KCNMB2, ZMAT3, PIK3CA, KCNMB3, ZNF639, MFN1, GNB4, ACTL6A, ZMAT3, PIK3CA, ZNF639, MFN1, PDX3 chr3:178525198-179754387 MRPL47, NDUFB5, USP13, PEX5L ACTL6A, MRPL47, NDUFB5 PDX14 chr6:17898411-19837986 NHLRC1,TPMT, KDM1B, DEK, RNF144B ND VSTM2A, SEC61G, EGFR, LANCL2, VOPP1, SEPT14, MRPS17_4, ZNF713, PDX14 chr7:54610424-56082864 ND GBAS PDX6 chr7: 54610424-55272949 VSTM2A, SEC61G, EGFR SEC61G, EGFR PDX6 chr11:82444771-82443491 ANO1, FADD, PPFIA1, CTTN ANO1, FADD, PPFIA1, CTTN PDX7 chr12: 25261482-25679033 CASC1, LYRM5, KRAS, LYRM5, KRAS AC026310.1, C12orf77, LRMP, CASC1, LYRM5, KRAS, AC087239.1, IFLTD1, PDX13 chr12:24970942-26492330 ND RASSF8, BHLHE41, SSPN CTB-133G6.1, CTD-2207O23.3_3, ARHGEF18, PEX11G, C19orf45, ZNF358, ARHGEF18, PEX11G, C19orf45, PDX5 chr19:34663668-34973546 MCOLN1, PNPLA6 ZNF358, MCOLN1, PNPLA6 ND, no data Selection of regions/genes with intragenic homozygous deletions PDX Id REGION GENES CANDIDATE TSGs PDX10 chr10: 89544227-90122482 ATAD1 (E1-5), KLLN (all) PTEN (all), RNLS (E5-7) PTEN PDX5 chr22:36960398-36983595 CACNG2 (E3-4) CTC-236F12.4 (all), AC022431.2 (all), MAP3K1 (all), SETD9 (all), MIER3 (all), MAP3K1, SETD9, MIER3, GPBP1, PDX5 chr5: 55753716-59189449 GPBP1 (all), ACTBL2 (all), PLK2 (all), GAPT (all), CTD-2117L12.1 (all), RAB3C PLK2, PDE4D CXADR (E4-7), BTG3 (all), C21orf91 (all), CHODL (all), TMPRSS15 (all), PDX4 chr21:18931294-22910278 CXADR, BTG3, C21orf91, CHODL NCAM2 (all) PDX6 chr1: 98039316-98060722 DPYD (E9-11) DPYD PDX10 chr2: 11591777-11932132 E2F6 (E1-4), GREB1 (all), NTSR2 (all), LPIN1 (E1-14) E2F6, LPIN1 PDX9 chr19:4233126-4237085 EBI3 (E3-5) PDX9 chr6:139206643-139206967 ECT2L (E16-17) PDX7 chr5:49695107-50687275 EMB (E1-9), PARP8 (all), ISL1 (E1-5) EMB, PARP8 PDX6 ch3:71096093-71179834 FOXP1 (E1-5) FOXP1 PDX4 chr6: 29910534-29913232 HLA-A (all) HLA-A IFNA1 (all), IFNE (all), MTAP(all), C9orf53 (all), CDKN2A (all), CDKN2B (all), PDX9 chr9: 21440507-22029516 CDKN2A RP11-145E5.5 (all), DMRTA1 (all) IFNW1 (all), IFNA21 (all), IFNA4 (all), IFNA7 (all), IFNA10 (all), IFNA16(all), PDX1 chr9:21440507-22029516 CDKN2A IFNA17 (all), IFNA14 (all), IFNA5 (all), KLHL9 (all), IFNA13 (all), IFNA2 PDX11 chr19:10597328-10610709 KEAP1 (all) KEAP1 PDX4 chr21:15481314-15755440 LIPI (all), RBM11 (all), HSPA13 (all) RBM11, HSPA13 PDX1 chr2: 141762904-142012210 LRP1B (E4-15) PDX9 chr2:141762904-141816623 LRP1B (E9-15) PDX7 chr2:135075100-135076310 MGAT5 (E4-5) MGAT5 PDX10 chr8:15967594-16035497 MSR1 (E5-10) PDX5 chr13:30072555-30088720 MTUS2 (E12-14), SLC7A1 (E13) SLC7A1 PDX10 chr20:8352029-8770908 PLCB1 (E3-31) PLCB1 PDX5 chr10: 89653782-89653866 PTEN (E2) PTEN PDX3 chr9:77112893-77761657 RORB (all), TRPM6 (all), C9orf40 (all), C9orf41 (all), NMRK1 (all), OSTF1 (all) C9orf40, C9orf41, NMRK1, OSTF1 PDX6 chr6: 152541977-152542694 SYNE1 (E118-119) PDX5 chr13:19748003-19755887 TUBA3C (all), RP11-408E5.4 (all) PDX3 chr19:22255611-22256366 ZNF257 (E2-3) Table 1SC. Selection of TSG and TSG candidates GENE PROTEIN NAME PDX CHROMPOSITION REF ALT MUTATION Zygosity status Notes ARID1A AT-rich interactive domain-containing protein 1A 9 chr1 27099479 G T SPLICE_SITE_DONOR HOMOZYGOUS ATAD2B ATPase family AAA domain-containing protein 2B 8 chr2 24042732 C A E718* HOMOZYGOUS ATG4D Cysteine protease ATG4D 14 chr19 10662763 CTGTG CTG FRAME_SHIFT HOMOZYGOUS ATM Serine-protein kinase ATM 7 chr11 108203488 G T SPLICE_SITE_ACCEPTOR HETEROZYGOUS ATM Serine-protein kinase ATM 7 chr11 108099967 C G S83* HETEROZYGOUS B2M Beta-2-microglobulin 5 chr15 45007752 G T E67* HOMOZYGOUS BAZ2B Bromodomain adjacent to zinc finger domain protein 2B 9 chr2 160239091 CTTTTTT CTTTTTTT FRAME_SHIFT HOMOZYGOUS CADM4 Cell adhesion molecule 4 9 chr19 44131912 AC A FRAME_SHIFT HOMOZYGOUS CAMSAP1 Calmodulin-regulated spectrin-associated protein 1 10 chr9 138713929 C A E582*;E860*;E871* HOMOZYGOUS CDKN2A Cyclin-dependent kinase inhibitor 2A 6 chr9 21971051 GC G FRAME_SHIFT HOMOZYGOUS CDKN2A Cyclin-dependent kinase inhibitor 2A 8 chr9 21971108 C A D84Y;R139L;R98L HOMOZYGOUS CDKN2A Cyclin-dependent kinase inhibitor 2A 10 chr9 21971110 T A H83L HOMOZYGOUS CDKN2A Cyclin-dependent kinase inhibitor 2A 14 chr9 GCG GC FRAME_SHIFT HOMOZYGOUS Identified by inspecting IGV CHD5 Chromodomain-helicase-DNA-binding protein 5 6 chr1 6214762 G A Q235* HOMOZYGOUS CIZ1 Cip1-interacting zinc finger protein 12 chr9 130947844 AG A FRAME_SHIFT HOMOZYGOUS CNOT10 CCR4-NOT transcription complex subunit 10 3 chr3 32811377 A G SPLICE_SITE_ACCEPTOR HOMOZYGOUS CNTNAP2 Contactin-associated protein-like 2 4 chr7 147183134 G A SPLICE_SITE_DONOR HOMOZYGOUS CROCC Rootletin 10 chr1 17285153 G T E1195*;E1314* HOMOZYGOUS CTSL2 Cathepsin L2 1 chr9 99795310 G T S309* HOMOZYGOUS DDX41 Probable ATP-dependent RNA helicase DDX41 5 chr5 176943196 T A SPLICE_SITE_ACCEPTOR HOMOZYGOUS DENND2C DENN domain-containing protein 2C 10 chr1 115137093 C A SPLICE_SITE_DONOR HOMOZYGOUS FAM83G Sodium/glucose cotransporter 5 10 chr17 18882980 T A R233* HOMOZYGOUS FBXW7 F-box/WD repeat-containing protein 7 8 chr4 153247244 C T D344N;D402N;D440N;D520N HOMOZYGOUS FBXW7 F-box/WD repeat-containing protein 7 11 chr4 153249393 G A S286F;S344F;S382F;S462F HOMOZYGOUS GALNT2 Polypeptide N-acetylgalactosaminyltransferase 2 3 chr1 230410196 G A W444*;W482* HOMOZYGOUS GM2A Ganglioside GM2 activator 7 chr5 150646307 G T E102*;E87* HOMOZYGOUS GRWD1 Glutamate-rich WD repeat-containing protein 1 10 chr19 48956209 ACCC AC FRAME_SHIFT HOMOZYGOUS INSL4 Early placenta insulin-like peptide 1 chr9 5233851 G T G132* HOMOZYGOUS KEAP1 Kelch-like ECH-associated protein 1 9 chr19 10602328 G T G417V;G417V HOMOZYGOUS KEAP1 Kelch-like ECH-associated protein 1 10 chr19 10610136 C A E192* HOMOZYGOUS LTBP2 Latent-transforming growth factor beta-binding protein 2 14 chr14 74975424 C A E1179* HETEROZYGOUS LTBP2 Latent-transforming growth factor beta-binding protein 2 14 chr14 97873937 C T W1274*
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    Stem Cells International Stem Cells and Ion Channels Guest Editors: Stefan Liebau, Alexander Kleger, Michael Levin, and Shan Ping Yu Stem Cells and Ion Channels Stem Cells International Stem Cells and Ion Channels Guest Editors: Stefan Liebau, Alexander Kleger, Michael Levin, and Shan Ping Yu Copyright © 2013 Hindawi Publishing Corporation. All rights reserved. This is a special issue published in “Stem Cells International.” All articles are open access articles distributed under the Creative Com- mons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Editorial Board Nadire N. Ali, UK Joseph Itskovitz-Eldor, Israel Pranela Rameshwar, USA Anthony Atala, USA Pavla Jendelova, Czech Republic Hannele T. Ruohola-Baker, USA Nissim Benvenisty, Israel Arne Jensen, Germany D. S. Sakaguchi, USA Kenneth Boheler, USA Sue Kimber, UK Paul R. Sanberg, USA Dominique Bonnet, UK Mark D. Kirk, USA Paul T. Sharpe, UK B. Bunnell, USA Gary E. Lyons, USA Ashok Shetty, USA Kevin D. Bunting, USA Athanasios Mantalaris, UK Igor Slukvin, USA Richard K. Burt, USA Pilar Martin-Duque, Spain Ann Steele, USA Gerald A. Colvin, USA EvaMezey,USA Alexander Storch, Germany Stephen Dalton, USA Karim Nayernia, UK Marc Turner, UK Leonard M. Eisenberg, USA K. Sue O’Shea, USA Su-Chun Zhang, USA Marina Emborg, USA J. Parent, USA Weian Zhao, USA Josef Fulka, Czech Republic Bruno Peault, USA Joel C. Glover, Norway Stefan Przyborski, UK Contents Stem Cells and Ion Channels, Stefan Liebau,
  • Bortezomib Inhibits Lung Fibrosis and Fibroblast Activation Without Proteasome

    Bortezomib Inhibits Lung Fibrosis and Fibroblast Activation Without Proteasome

    bioRxiv preprint doi: https://doi.org/10.1101/2021.02.26.433086; this version posted February 26, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Bortezomib inhibits lung fibrosis and fibroblast activation without proteasome 2 inhibition 3 4 Loka Raghu Kumar Penke, Jennifer Speth, Scott Wettlaufer, Christina Draijer and Marc 5 Peters-Golden1* 6 7 Affiliations 8 Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, 9 University of Michigan, Ann Arbor, Michigan, USA. 10 1 Graduate Program in Immunology, University of Michigan, Ann Arbor, Michigan, USA. 11 12 Correspondence: 13 Marc Peters-Golden, M.D. 14 6301 MSRBIII, 1150 W. Medical Center Drive, Ann Arbor, MI 48109-5642 15 Tel: 734-936-5047 16 Fax: 734-764-4556 17 Email: [email protected] 18 Author Contributions: L.R.K.P. planned and performed experiments, analyzed the 19 data, organized data for presentation, and wrote the manuscript. J.M.S, S.H.W and C.D 20 performed experiments. M.P.-G. planned experiments, analyzed data, and wrote the 21 manuscript. 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.26.433086; this version posted February 26, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license.
  • Ion Channels

    Ion Channels

    UC Davis UC Davis Previously Published Works Title THE CONCISE GUIDE TO PHARMACOLOGY 2019/20: Ion channels. Permalink https://escholarship.org/uc/item/1442g5hg Journal British journal of pharmacology, 176 Suppl 1(S1) ISSN 0007-1188 Authors Alexander, Stephen PH Mathie, Alistair Peters, John A et al. Publication Date 2019-12-01 DOI 10.1111/bph.14749 License https://creativecommons.org/licenses/by/4.0/ 4.0 Peer reviewed eScholarship.org Powered by the California Digital Library University of California S.P.H. Alexander et al. The Concise Guide to PHARMACOLOGY 2019/20: Ion channels. British Journal of Pharmacology (2019) 176, S142–S228 THE CONCISE GUIDE TO PHARMACOLOGY 2019/20: Ion channels Stephen PH Alexander1 , Alistair Mathie2 ,JohnAPeters3 , Emma L Veale2 , Jörg Striessnig4 , Eamonn Kelly5, Jane F Armstrong6 , Elena Faccenda6 ,SimonDHarding6 ,AdamJPawson6 , Joanna L Sharman6 , Christopher Southan6 , Jamie A Davies6 and CGTP Collaborators 1School of Life Sciences, University of Nottingham Medical School, Nottingham, NG7 2UH, UK 2Medway School of Pharmacy, The Universities of Greenwich and Kent at Medway, Anson Building, Central Avenue, Chatham Maritime, Chatham, Kent, ME4 4TB, UK 3Neuroscience Division, Medical Education Institute, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY, UK 4Pharmacology and Toxicology, Institute of Pharmacy, University of Innsbruck, A-6020 Innsbruck, Austria 5School of Physiology, Pharmacology and Neuroscience, University of Bristol, Bristol, BS8 1TD, UK 6Centre for Discovery Brain Science, University of Edinburgh, Edinburgh, EH8 9XD, UK Abstract The Concise Guide to PHARMACOLOGY 2019/20 is the fourth in this series of biennial publications. The Concise Guide provides concise overviews of the key properties of nearly 1800 human drug targets with an emphasis on selective pharmacology (where available), plus links to the open access knowledgebase source of drug targets and their ligands (www.guidetopharmacology.org), which provides more detailed views of target and ligand properties.
  • Transcriptional and Post-Transcriptional Regulation of ATP-Binding Cassette Transporter Expression

    Transcriptional and Post-Transcriptional Regulation of ATP-Binding Cassette Transporter Expression

    Transcriptional and Post-transcriptional Regulation of ATP-binding Cassette Transporter Expression by Aparna Chhibber DISSERTATION Submitted in partial satisfaction of the requirements for the degree of DOCTOR OF PHILOSOPHY in Pharmaceutical Sciences and Pbarmacogenomies in the Copyright 2014 by Aparna Chhibber ii Acknowledgements First and foremost, I would like to thank my advisor, Dr. Deanna Kroetz. More than just a research advisor, Deanna has clearly made it a priority to guide her students to become better scientists, and I am grateful for the countless hours she has spent editing papers, developing presentations, discussing research, and so much more. I would not have made it this far without her support and guidance. My thesis committee has provided valuable advice through the years. Dr. Nadav Ahituv in particular has been a source of support from my first year in the graduate program as my academic advisor, qualifying exam committee chair, and finally thesis committee member. Dr. Kathy Giacomini graciously stepped in as a member of my thesis committee in my 3rd year, and Dr. Steven Brenner provided valuable input as thesis committee member in my 2nd year. My labmates over the past five years have been incredible colleagues and friends. Dr. Svetlana Markova first welcomed me into the lab and taught me numerous laboratory techniques, and has always been willing to act as a sounding board. Michael Martin has been my partner-in-crime in the lab from the beginning, and has made my days in lab fly by. Dr. Yingmei Lui has made the lab run smoothly, and has always been willing to jump in to help me at a moment’s notice.
  • Figure S1. HAEC ROS Production and ML090 NOX5-Inhibition

    Figure S1. HAEC ROS Production and ML090 NOX5-Inhibition

    Figure S1. HAEC ROS production and ML090 NOX5-inhibition. (a) Extracellular H2O2 production in HAEC treated with ML090 at different concentrations and 24 h after being infected with GFP and NOX5-β adenoviruses (MOI 100). **p< 0.01, and ****p< 0.0001 vs control NOX5-β-infected cells (ML090, 0 nM). Results expressed as mean ± SEM. Fold increase vs GFP-infected cells with 0 nM of ML090. n= 6. (b) NOX5-β overexpression and DHE oxidation in HAEC. Representative images from three experiments are shown. Intracellular superoxide anion production of HAEC 24 h after infection with GFP and NOX5-β adenoviruses at different MOIs treated or not with ML090 (10 nM). MOI: Multiplicity of infection. Figure S2. Ontology analysis of HAEC infected with NOX5-β. Ontology analysis shows that the response to unfolded protein is the most relevant. Figure S3. UPR mRNA expression in heart of infarcted transgenic mice. n= 12-13. Results expressed as mean ± SEM. Table S1: Altered gene expression due to NOX5-β expression at 12 h (bold, highlighted in yellow). N12hvsG12h N18hvsG18h N24hvsG24h GeneName GeneDescription TranscriptID logFC p-value logFC p-value logFC p-value family with sequence similarity NM_052966 1.45 1.20E-17 2.44 3.27E-19 2.96 6.24E-21 FAM129A 129. member A DnaJ (Hsp40) homolog. NM_001130182 2.19 9.83E-20 2.94 2.90E-19 3.01 1.68E-19 DNAJA4 subfamily A. member 4 phorbol-12-myristate-13-acetate- NM_021127 0.93 1.84E-12 2.41 1.32E-17 2.69 1.43E-18 PMAIP1 induced protein 1 E2F7 E2F transcription factor 7 NM_203394 0.71 8.35E-11 2.20 2.21E-17 2.48 1.84E-18 DnaJ (Hsp40) homolog.